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R
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38
library(scDblFinder) samples_dir <- "C:/Users/mauri/Desktop/scRNAseqR/data/samples/sakshi NGN2 microglia" sample_name <- "NSM" data.data <- Seurat::Read10X(data.dir = file.path(samples_dir, sample_name, "filtered_feature_bc_matrix"), strip.suffix = TRUE) data <- Seurat::CreateSeuratObject(counts = data.data, project ...
f1ca8a1d0fd7af08bf92f453992d406318e2bde3b042c04749998c977f7af172
R
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43
library(dplyr) dat <- read.csv("F:\\Manuscript\\ATAC\\figure\\ori_Tissue_stage_specific\\ReadCount\\ATAC-Tissue_specific.peaks.csv", header = T) %>% arrange(.,Var1) AnnotationFile <- read.csv("F:\\Manuscript\\ATAC\\data\\AllPeakList.merged.ReadCount.PeakToGene_Feature.csv", header = T) AnnotationFile_f <- filt...
bb74c451b0b19e9cf44761046cd9743205e4f457e9f2fcb312546b77a10b62ee
R
1,632
61
library(colorspace) library(doParallel) library(dplyr) library(foreach) library(grid) library(httr) library(magick) library(readr) library(spacesXYZ) library(stringr) library(tibble) # This file is created by 1_Parse_Articles_from_XML.R. articles_filepath = "all_eLife_articles.tsv" # This is where the images download...
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R
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test_that("data reduced by keywords", { pdf(NULL) # to prevent generating an empty RPlots.pdf path <- tempdir() on.exit(unlink(path)) write_testyamlfile(path, analyser = c("TimeSlots", "Responses"), changes = c("DataSettings$Stimulus$Time = 1", "DataSettin...
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R
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#!/usr/bin/R # -------------------------------------------------- # Functions to process to individual / region level: # -------------------------------------------------- library(cbrbase) aggregatePsbulkIndRegion = function(plt.data){ # Aggregate at the projid x region level (should be single-set already): p...
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R
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#bigsnpr to calculate pca #conda activate /mnt/analysis-psp/users/timchang/programs/miniconda/R3.6.3 .libPaths('/mnt/analysis-psp/users/timchang/programs/R/3.6.3/') library(bigsnpr);library(bigreadr);library(data.table) options(bigstatsr.check.parallel.blas = FALSE) options(default.nproc.blas = NULL) geno_path='/mnt...
1cda19d6bfc4d3143d90308361d3f0ea154b06704a67643f685ebc30658de70f
R
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options(bitmapType='cairo') args <- commandArgs(TRUE) wd<-args[1] replicateFile<-args[2] sampleFile<-args[3] sampleName<-args[4] setwd(wd) rep.data<-read.table(replicateFile,header=T,sep="\t") rep.data.x<-as.numeric(rep.data[,1]) sample.data<-read.table(sampleFile,header=T,sep="\t") sample.data.x<-as....
d378bbb449048c0136c1398bd28fd1e4337a74f8d625ee96422e2c4d5ebfe5f8
R
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library(tidyverse) labeled_data = read_tsv("PMC_Selected_Articles_labeled.tsv") %>% mutate(image_file_path = str_c("PMC_Images/", Image_Number, "/original.jpg")) %>% dplyr::rename(Class = `Conclusion (5 types listed in drop down)`) %>% select(image_file_path, Class) group_by(labeled_data, Class) %>% summarize...
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R
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library(fmri.pipeline) # note that HPC settings like nodes and batch_code are ignored for local scheduler w <- R_batch_job$new( batch_directory="~/batch_test", job_name="step1", n_nodes=1, n_cpus=1, cpu_time="10:00", r_code=c( "Sys.sleep(100)", "print('hi')", "x <- 2+2" ), r_packages=c("lme4...
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R
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library(testthat) test_that("compose_l2_model_data skips identical session-level duplicates already present in run_data", { gpa <- create_mock_gpa() gpa$run_data$tr <- 1 gpa$run_data$session_label <- paste0("ses", gpa$run_data$session) gpa$session_data <- unique(gpa$run_data[, c("id", "session", "tr", "sessio...
641f20762ece13adf4b822055648b633e94682e5b8c601e8db444a35bd106f84
R
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test_that("YAML contrasts are nested and roundtrip into contrast_spec", { skip_if_not_installed("yaml") gpa <- create_mock_gpa(n_subjects = 1, n_runs = 1, n_trials = 5, output_directory = tempdir()) spec <- list( onsets = list("onset"), durations = list("duration"), events = list( stim = list(...
ef52964c4125d4b50533106b28a463a43f2c97fe06f230739c0bb8a23b77aa34
R
1,663
58
require(GenomicSEM) library(data.table) NAME <- "MetS" ldsc.out <- "../2_ldsc/ldscout/MetS_ldscout.RData" sumstat.out <- "../7_sumstat/MetS_sumstat.RData" load(ldsc.out) load(sumstat.out) model <- "F1 =~ 1*BMI_GIANT18 + WC_Watanabe_UKB F2 =~ 1*T2D_meta_FinR7_Mahajan22_MVP + FG_Lagou_MAGIC_noUKB + HYP_meta_F...
e5d13097fc936d01320f2643d6695d753eb4ce48fc1b1b43f6f60220f7707bf2
R
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library(jsonlite) library(mediation) # ---------------------------------------------------------------------- # Configuration and data loading setwd(dirname(rstudioapi::getActiveDocumentContext()$path)) config <- fromJSON("config.json") source("helpers.R") dataframes_dir <- config$dataframes_dir tables_dir <- config...
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R
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#' @keywords internal #' @noRd #' .subset <- function(.data, .column) { # Make col names .column <- enquo(.column) # Check if column present if(quo_names(.column) %in% colnames(.data) %>% all %>% `!`) stop("sccomp says: some of the .column specified do not exist in the input data frame.") .data %>% ...
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R
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require(testthat) require(Cardinal) context("SpatialResults") test_that("SpatialResults + ResultsList", { require(datasets) fit1 <- lm(mpg ~ disp, data=mtcars) fit2 <- lm(mpg ~ disp + wt, data=mtcars) fit3 <- lm(mpg ~ disp + hp, data=mtcars) n <- 100 mz <- seq(500, 510, length.out=n) mdf <- MassDataFrame(mz=...
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R
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# on cluster, initiate qlogin session with 16 cores. # qlogin -q trapnell-login.q -l mfree=4G -pe serial 16 # start R (4.0.0) # basepath = "/net/trapnell/vol1/home/bge/novaseq/210111_Riza_sciATAC3/analyze_out/" basepath = "/net/trapnell/vol1/HuBMAP/novaseq/210111_Riza_sciATAC3_split_sample/analyze_out/" # out_path =...
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R
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52
library("harmony") library("Seurat") library("tidyverse") setwd("./Step4.IterativeCleaning/CleanedClusters.R2") SeuratObjs <- list.files(pattern = ".rds") # load the files Seurat.list <- lapply(SeuratObjs, readRDS) # Merge into one single Seurat object Seurat.SNC <- merge(x = Seurat.list[[1]], y = unlist(lapply(2:7,...
932b7df409e351c42e5bb268cba6949f6f36f98583d0e95358944664e682e3dd
R
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# on cluster, initiate qlogin session with 16 cores. # qlogin -q trapnell-login.q -l mfree=4G -pe serial 16 # start R (4.0.0) # basepath = "/net/trapnell/vol1/home/bge/novaseq/210111_Riza_sciATAC3/analyze_out/" basepath = "/net/trapnell/vol1/HuBMAP/novaseq/210111_Riza_sciATAC3_split_sample/analyze_out/" # out_path =...
ce073e5ff65bafae0a4bfa5991a7081a3d8d57cb886931e6cabbf5f07e4dea24
R
1,684
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--- title: "DeepComBat package tester" output: html_document --- # Preprocessing ```{r} library(tidyverse) library(torch) library(neuroCombat) library(CovBat) #General library(tidyverse) library(reshape2) library(matlib) library(matrixStats) library(forcats) #DeepComBat library(torch) library(torchvision) library(ne...
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R
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library(data.table) library(dplyr) for(tis in c("Brain", "Lung", "Muscle", "Heart")){ for(dir in c("m6A2ha", "ha2m6A")){ SMR_res <- data.frame() for(i in 1:22){ SMR_res0 <- fread(paste0("~/SMR/", tis, "_", dir, "_smr/chr", i, ".msmr"), sep = "\t", header = TRUE) SMR_res <- bind_rows(SMR_res, SMR_res0) } ...
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R
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#' @name palette_colorblind #' @aliases palette_colorblind colors,palette_colorblind #' #' @title Color suggestions for `hyperSpec` users #' #' @description #' In \pkg{hyperSpec} the user may use any color name/format known to `R`. #' An important fact to keep in mind is that most people with normal vision #' cannot di...
3da621c553eec4746e06523f060efc90285c657fb62a2585d7e866b6af5102ad
R
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test_that("mat2df converts numeric matrices like matrix melt", { mat <- matrix(as.numeric(seq_len(6)), nrow = 2) row_key <- c(1L, 2L, 1L, 2L, 1L, 2L) col_key <- c(1L, 1L, 2L, 2L, 3L, 3L) signal <- as.numeric(seq_len(6)) expect_identical( fmri.pipeline:::mat2df(mat, value_name = "signal", varnames = c("vn...
fac5a7f52b27d4c482ef788f0f529acc43bc5e20e49a95442697a749a2bd410b
R
1,695
45
library(testthat) test_that("old .sample/.cell_group argument style works with placeholders", { skip_cmdstan() suppressPackageStartupMessages({ library(dplyr) }) test_data <- tibble::tribble( ~orig.ident, ~micro_conf, ~sex, ~age, ~manual_fine, ~count, "NS031", "yes", "F", 31, "Memory B cells", 1...
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R
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# on cluster, initiate qlogin session with 16 cores. # qlogin -q trapnell-login.q -l mfree=4G -pe serial 16 # start R (4.0.0) # basepath = "/net/trapnell/vol1/home/bge/novaseq/210111_Riza_sciATAC3/analyze_out/" basepath = "/net/trapnell/vol1/HuBMAP/novaseq/210111_Riza_sciATAC3_split_sample/analyze_out/" # out_path =...
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R
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# Function ------------------------------------------------------------------- #' @name DEPRECATED-options #' @concept deprecated #' #' @title (DEPRECATED) #' Options for package \pkg{hyperSpec} #' #' @description #' #' These functions are **deprecated**. #' Use [hy_get_option()], [hy_get_options()], or [hy_se...
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R
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# This script reformats the output bed files from module 01 with raw call evidence # to match desired input for de novo SV filtering # Define args and load libraries args = commandArgs(trailingOnly=TRUE) require(plyr) library(data.table) library(tidyverse) input_bed <- args[1] input_ped <- args[2] out_probands <- arg...
21d44798bca70733eb95f348e1e74bda32e56639c32d1573fa5f4cafbe3e6232
R
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# plot the number of transcripts unique and common to DN and NeuN p3 <- class.files$targ_sorted %>% group_by(associated_gene, dataset) %>% tally() %>% ggplot(., aes(x = associated_gene, y = n, fill = dataset)) + geom_bar(stat = "identity", position = position_dodge()) + labs(x = "Gene", y = "Number of isoforms") ...
7acb48813ed2556d60048014ec4217ab9ca8b79ecffc1490bb6bec58002ae531
R
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require(GenomicSEM) library(data.table) NAME <- "MetS_noUKB" ldsc.out <- "../2_ldsc/ldscout/MetS_noUKB_ldscout.RData" sumstat.out <- "../7_sumstat/MetS_noUKB_sumstat.RData" load(ldsc.out) load(sumstat.out) model <- "F1 =~ 1*BMI_GIANT15_noUKB + WC_GIANT_noUKB F2 =~ 1*T2D_meta_FinR7_MVP_noUKB + FG_Lagou_MAGIC...
1e527c5010eec66e71c0bd91679c19d06e0205ad926e804ab0dad41d8ff20679
R
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#################################################################################################### ### ### File: 07_hmm_illustration.r ### Purpose: Creating a illustration for the HMM models idea ### Authors: Gabriel Rodrigues Palma, Rafael Moral ### Date: 10/04/23 ### ##########################################...
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R
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library(tidyr) library(dplyr) phenotype_suffix = snakemake@params[['phenotype_suffix']] linear_model_res_path = snakemake@params[['linear_model_res_path']] all_combined_metrics = readRDS(file.path(linear_model_res_path, 'plotting_data', paste0('all_recomputed_metrics_test_', phenotype_suffix,'.Rds'))) baseline_btyp...
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#! /usr/bin/env Rscript ##meant to generate cutoffs for different copy states## ##first input is a PE file## ## second input is output filename for final matrix of cutoffs## args <- commandArgs(TRUE) file=args[1] outputfile=args[2] median_matrix=read.table(file) output_matrix=matrix(,ncol=3,nrow=5) for (i in 0:3) ...
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R
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options(bitmapType='cairo') args <- commandArgs(TRUE) wd<-args[1] inputFile<-args[2] setwd(wd) data<-read.table(inputFile,header=F,sep="\t") data.x<-as.numeric(data[,1]) x.tmp<-sort(data.x) x.tmp.size<-length(x.tmp) x.tmp.topIndex=floor(x.tmp.size*.99) x.tmp.bottomIndex=floor(x.tmp.size*.01)+1 x.tmp....
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R
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#' @name DEPRECATED-guess.wavlength #' @concept deprecated #' #' @title (DEPRECATED) #' Guess wavelengths from character vector #' #' @description #' #' These \pkg{hyperSpec} functions are **deprecated** and not maintained any #' more. You should not use these. #' Currently they are present due to back-compatibi...
67963d7c63a895bcf22fa9154466e4911b78c8a21600cde5da2e374fef1cc21f
R
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#' Helper function to fit betabin model while handling common warnings/errors #' #' Just wraps betabin to return either the fit or warning description. Warnings #' and errors occur when the model doesn't converge. These will be flagged for #' removing downstream. #' #' @description This function fits a beta-binomial mo...
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R
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require(testthat) require(Cardinal) context("read/write imzML and Analyze 7.5") test_that("read/write continuous", { path <- CardinalIO::exampleImzMLFile("continuous") mse <- readImzML(path) expect_true(is(mse, "MSImagingExperiment")) path2 <- paste0(tempfile(), ".imzML") writeImzML(mse, path2) mse2 <- readI...
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R
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options(bitmapType='cairo') args <- commandArgs(TRUE) wd<-args[1] vector1File<-args[2] vector2File<-args[3] sampleName<-args[4] setwd(wd) vector1.name<-basename(vector1File) vector1.data<-read.table(vector1File,header=T,sep="\t") vector1.data.x<-as.numeric(vector1.data[,1]) vector2.name<-basename(vect...
dffd5f5aa6a7f79c390c7ac517fab9f8d332bd467e47cb6d8054b1a1ad2fe9e1
R
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library(GenomicRanges) library(tidyr) library(dplyr) library(regioneR) setwd('~/hail/hail-files/SV') inh <- read.table('annot.inhsubset.filter.bed', header=TRUE, comment.char = '') colnames(inh)[1] <- 'chr' inhGR <- toGRanges(inh) args = commandArgs(trailingOnly=TRUE) bed <- args[1] CN <- args[2] pheno <- args[3] outf...
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## ----------Script----------------- ## ## Author: Szi Kay Leung (S.K.Leung@exeter.ac.uk) ## Purpose: Generate post-sqanti plots for summary of ONT targeted mouse transcriptome datasets ## ## -------------------------------- ## ---------- packages ----------------------------------------- suppressMessages(library("d...
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R
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#' @name DEPRECATED-read.mat.Witec #' @concept moved to hySpc.read.mat #' #' @title (DEPRECATED) #' Read `.mat` file into `hyperSpec` object #' #' @description #' #' These data input functions are **deprecated** and they will be removed in #' the next release of \pkg{hyperspec} package. #' Now functions in packa...
b817f7431bf5b7d39888c5ea98c972501b82da03b608f36e8cc51947a6bb331c
R
1,753
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options(bitmapType='cairo') minor.ticks.axis <- function(ax,n,t.ratio=0.5,mn,mx){ lims <- par("usr") if(ax %in%c(1,3)) lims <- lims[1:2] else lims[3:4] major.ticks <- seq(floor(mn),ceiling(mx),1) labels <- sapply(major.ticks,function(i) as.expression(bquote(10^ .(i))) ) a...
124d56efda6d3fe35934e433a1af3295ab6d48f419445f4046af88a6166ff780
R
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## <----------------- LOAD LIBRARIES library(data.table) ## <--------------- MAIN ## Store command line argument to variable args <- commandArgs(trailingOnly=TRUE) iter <- as.numeric(args[1]) # current iteration message(iter) ## Get HPO terms with proband N from hpo_sig get_hpo_n <- fread("/path/to/hpo_prs_int_cou...
cd153adcb27ebee30d6804b006b888908dccffcb3bf68eea7d7fb60d9a7e6642
R
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test_that("build_l3_models warns when L2 models are not yet defined", { gpa <- create_mock_gpa( n_subjects = 3, n_runs = 2, include_l1_models = TRUE, include_l2_models = FALSE, include_l3_models = FALSE ) spec <- list( l3_models = list( l3_age = list( name = "l3_age", ...
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#' Helper function to fit quasibinomial model while handling common warnings/errors #' #' Just wraps glm to return either the fit or warning description. Warnings #' and errors occur when the model doesn't converge. These will be flagged for #' removing downstream. #' #' @description This function fits a quasibinomial ...
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R
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splitgenome <- function(CHR, POS, ref.CHR, ref.breaks, details=T, right=TRUE) { #' Function to split a set of SNPs by their position using a reference #' Break points should be given for the reference by chromosome #' @keywords internal CHR <- as.character(CHR) ref.CHR <- as.character(ref.CHR) POS <- as....
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#1. assign methylation level to different methylation group rank #methylation level plot rank #<5% 0 #5-15% 10 #15-25% 20 #25-35% 30 #35-45% 40 #45-55% 50 #55-65% 60 #65-75% 70 #75-85% 80 #85-95% 90 #>95% 100 #sample methylation level visualization with different methylation group rank ##line plot ...
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require(GenomicSEM) library(data.table) NAME <- "MetS_independent" ldsc.out <- "../2_ldsc/ldscout/MetS_ldscout.RData" sumstat.out <- "../7_sumstat/MetS_sumstat.RData" load(ldsc.out) load(sumstat.out) model <- "F1 =~ 1*BMI_GIANT18 + WC_Watanabe_UKB F2 =~ 1*T2D_meta_FinR7_Mahajan22_MVP + FG_Lagou_MAGIC_noUKB ...
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R
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#= # Introduction ---- #' This file was adapted from < https://github.com/yihui/knitr/blob/master/inst/examples/knitr-spin.R>. #' This is a special R script which can be used to generate a report. You can #' write normal text in roxygen comments. #' #= # Quick Start ---- #= ## Options ---- #' First we set up some optio...
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R
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#' Color coding legend for factors #' #' Modifies a list of lattice arguments (as for [lattice::levelplot()], etc.) according to #' the factor levels. The colorkey will shows all levels (including unused), and the drawing colors #' will be set accordingly. #' #' `trellis.factor.key` is used during `levelplot`-based plo...
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R
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##AGE gene enrichement analysis dat <- read.csv("F:\\ATAC\\processFiles\\POE05112023\\MG_AllGenomePeakReadNumber.NoDistal.GroupByGene.csv", header = T, row.names = 1) geneList <- rownames(dat) AGE <- read.csv("F:\\RNA_20221101\\02_ModifiedGenome\\D_AGEgeneSelection\\result\\AGE.list.csv", header = T, row.names = 1...
341ef8b7c36735ce4aeb1ca81a715326f7180779b7e4cea1c23676b1f7728dc6
R
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setwd("F:\\Manuscript\\RNA\\Ori\\figure\\AGE_POE_TissuesVenn") library(VennDiagram) ##POE Brain <- read.table("POE_Brain_bias2.txt", header=F)[,1] Liver <- read.table("POE_Liver_bias2.txt", header=F)[,1] Muscle <-read.table("POE_Muscle_bias2.txt", header=F)[,1] Placenta <-read.table("POE_Placenta_bias2.txt", ...
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R
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data <- qs::qread("C:/SynologyDrive/Projects/scRNAseqR/results/SB_V1/SB/SB.qs") selection_markers = c("MAP2", "DCX", "NEUROG2") # selection_markers = c("VIM", "S100B", "SOX9") # selection_markers = c("AIF1", "CSF1R", "SPI1") Seurat::Idents(data) <- data$seurat_clusters data <- Seurat::FindSubCluster( object = data, ...
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1,801
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require(GenomicSEM) library(data.table) NAME <- "MetS" log.dir <- "./logs/" dir.create(log.dir, showWarnings = FALSE) log.dir <- paste0(log.dir, NAME) dir.create(file.path(log.dir), showWarnings = FALSE) setwd(log.dir) sumstat.dir <- "../../gwas/EasyQC/results/" reference.file <- "reference.1000G.maf.0.005.txt" sav...
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R
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require(GenomicSEM) library(data.table) NAME <- "MetS_noUKB_independent" ldsc.out <- "../2_ldsc/ldscout/MetS_noUKB_ldscout.RData" sumstat.out <- "../7_sumstat/MetS_noUKB_sumstat.RData" load(ldsc.out) load(sumstat.out) model <- "F1 =~ 1*BMI_GIANT15_noUKB + WC_GIANT_noUKB F2 =~ 1*T2D_meta_FinR7_MVP_noUKB + FG...
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#bigsnpr to calculate pca #conda activate /mnt/analysis-psp/users/timchang/programs/miniconda/R3.6.3 #conda install r-codetools r-matrix .libPaths('/mnt/analysis-psp/users/timchang/programs/R/3.6.3/') #also install bigsnpra, RhpcBLASctl library(bigsnpr);library(bigreadr);library(data.table) geno_path='/mnt/analysis-ps...
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R
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#!/usr/bin/R # ----------------------------------------------------------- # Make the set of shared DEGs "All_All" for every combination # Updated: 03/27/22 # ----------------------------------------------------------- library(cbrbase) set_proj('DEVTRAJ', 'multiRegion') source(paste0(bindir, 'multiRegion/load_metadata....
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R
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setwd("/media/hansruedi/3b32f153-0651-4d56-b863-9b070ac7dd55/home/hansruedi/Brain_region_final") library(ggplot2) library(Seurat) Exc_part1 <- readRDS(file = "Ex_raw_set1_2_subset2000.rds") Exc_part2 <- readRDS(file = "Ex_raw_set2_2_subset2000.rds") Inh <- readRDS(file = "In_raw_mscores_integrated2_subset2000.rds"...
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R
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--- title: "Responses Guide for NosaAnalysis" author: "Julia Thüringer" date: '`r Sys.Date()`' output: html_document: default pdf_document: null vignette: > %\VignetteIndexEntry{Responses Guide for NosaAnalysis} %\VignetteEngine{knitr::rmarkdown} \usepackage[utf8]{inputenc} --- ### Description ```{r descrip...
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R
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library(osfr) source("validation/calculate_performance_function.R") library(readr) library(dplyr) library(ASySD) # get labelled duplicate sets from OSF project asysd_validation_project <- osf_retrieve_node("https://osf.io/2b8uq/") files <- osf_ls_files(asysd_validation_project) osf_download(files[7,], path="validation...
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R
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#!/usr/bin/env Rscript # This script is used to modify the SR bg info for common (freq > 50) SVs with SR support on both breakpoints # Sets PESR evidence metrics to 0 at common sites where there is SR support on both sides. library("optparse") option_list = list( make_option(c("-p","--pedfile"), type="charact...
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R
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rm(list=ls()) load("CPM_exp.RData") load("sig_res/markers100.RData") library(ssgsea.GBM.classification) source("~/ssgseaMOD.r") marker <- rep(1,length(markers100$mes)) names(marker) <- markers100$mes mod.generate(marker,out="mes.mod") marker <- rep(1,length(markers100$pn)) names(marker) <- markers100$pn mod.generate(m...
9901325daa6373d2310be193b69eccf53acd7ddb9eab1028a4e143b74887b743
R
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#!/usr/bin/R # ------------------------------------------------------ # Load all of the modules and save supplementary tables: # Updated 06/20/2022 # ------------------------------------------------------ library(cbrbase) set_proj('DEVTRAJ', 'multiRegion') if (!exists("cellmeta")){ source(paste0(sbindir, 'load_meta...
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R
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# Tmisc() # setwd(attachroot("/WORK/myRpackages/lassosum/inst/data")) # Tim.load(Rplink) # set.seed(1000) # chr22 <- attachroot("~/DATA/1000G/PLINK/chr22") # chr21 <- attachroot("~/DATA/1000G/PLINK/chr21") # subsample(bfile=chr21, out="chr21", maf=0.05, fill.missing.a2=T, # keep=logical.vector(1:200,2504), p=...
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R
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library('RColorBrewer') library('MAST') library('data.table') library('Seurat') library('biomaRt') library('lme4') setwd("./Projects/SNCSynuclein") # load the seurat object Seurat.SNC.final <- readRDS("Step5.FinalClustering/Seurat.SNC.final.rds") # set the levels for factors: Pathological Dx Seurat.SNC.final$Dx <- N...
fb7fbe923cc5a756c84d58ed25bfdb9c4892b7e21640ea1baa1b72320f33661d
R
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sink("06_cML_MA_DP.parallel.log") library(data.table) library(dplyr) library(MRcML) library(foreach) library(doParallel) n_parallel <- 5 dir_in <- "cml_ma_input/" dir_out <- "cml_ma_dp_result/" dir.create(dir_out, showWarnings = FALSE) # Metadata file_in_mr_result <- "tsmr_best_sig_result.csv" df_mr_result <- fread...
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R
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# Gather cognitive tests and confounds into one file. # Output: # pred_all.csv cognitive tests and confounds (i.e., Age, Gender). #Load packages. library(tidyverse) #Set outpath. subgroup <- 'full' outpath <- paste0('../outputs/c_cognition/',subgroup,'/') dir.create(outpath,recursive=T) #Read in subjects. subjects <...
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R
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#' --- #' title: "Generate a complete library range object" #' author: "Tomas Bjorklund" #' output: #' pdf_document: #' highlight: tango #' geometry: margin=0.7in #' --- #' This short script generates a lookup scoring table of the AAV plasmid library so that it follows the same structure as the mRNA samples so t...
471c3b4c3d8a1ebfd72bb9ac221358bf2a1905355390ca65aae809b2aaec2f92
R
1,847
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test_that("FLAME fallback TSV records are promoted to lgr estimation logs", { tmp <- withr::local_tempdir() feat_dir <- file.path(tmp, "cope1.feat") dir.create(feat_dir, recursive = TRUE) fallback_tsv <- file.path(feat_dir, "flame_runner_fallbacks.tsv") writeLines(c( "timestamp_utc\tpid\tflame12_status\t...
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R
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library(Seurat) library(ggplot2) library(ggpubr) options(future.globals.maxSize = 4000 * 1024^20) Sys.setenv('R_MAX_VSIZE'=2500000000000) dir<-read.csv(file="sample.lst") dataList=dir$orig.ident dataList <- lapply(X = dataList, FUN = function(x) { print (x) x <- readRDS(file=paste0( x,".rds")) }) #print ("d...
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R
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#' @name DEPRECATED-spc-rubberband #' @concept deprecated #' #' @title (DEPRECATED) #' Rubberband baseline correction #' #' @description #' These \pkg{hyperSpec} functions are **deprecated** and not maintained any #' more. You should not use these. #' Currently they are present due to back-compatibility reasons ...
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R
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#!/usr/bin/R # --------------------------------------------------- # Plot the RNAscope quantification for the thalamus: # Updated 12/07/2021 # --------------------------------------------------- library(cbrbase) set_proj('DEVTRAJ', 'multiRegion') source(paste0(bindir, 'multiRegion/load_metadata.R')) library(tidyr) l...
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R
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#' @concept utils .validate <- function(object) { ncol <- ncol(object@data$spc) if (is.null(ncol)) { ncol <- 0 } if (length(object@wavelength) != ncol) { return("Length of wavelength vector differs from number of data points per spectrum.") } TRUE } # Function --------------------------------...
d7d11f0ebfb32e0939d3eeb6f8dc0a3218023b932f9065e20221099ffd1e77c0
R
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# These functions contain some default arguments that worked # in the past for creating heat maps. # It also helps with some of the preprocessing. library(ComplexHeatmap) library(dplyr) library(tibble) library(magrittr) library(RColorBrewer) library(circlize) # Input matrix pretty much needs to be exactly the heatma...
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R
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library(tidyverse) library(ComplexHeatmap) library(circlize) library(RColorBrewer) library(colorRamp2) # Read in data protein_data <- read_xlsx("~/VSN_NA_imputed.xlsx") # Transform to format needed to make heatmap, scale and center data protein_mat_norm <- protein_data %>% column_to_rownames("Gene") protein_mat_no...
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R
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require(GenomicSEM) library(data.table) NAME <- "MetS_noUKB" log.dir <- "./logs/" dir.create(log.dir, showWarnings = FALSE) log.dir <- paste0(log.dir, NAME) dir.create(file.path(log.dir), showWarnings = FALSE) setwd(log.dir) # Place where the logs will be saved sumstat.dir <- "../../gwas/EasyQC/results/" reference.f...
37fee16530ffc98250347b4fb522e3ae3a4448ede5e534a34b264be2669cbc0a
R
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## ---- include = FALSE--------------------------------------------------------- knitr::opts_chunk$set( collapse = TRUE, comment = "#>" ) ## ----eval=FALSE--------------------------------------------------------------- # if (!requireNamespace("BiocManager", quietly = TRUE)) # install.packages("BiocManager") ...
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R
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options(bitmapType='cairo') args <- commandArgs(TRUE) name<-args[1] dataColumn<-as.numeric(args[2]) skip<-as.numeric(args[3]) minX<-as.numeric(args[4]) maxX<-as.numeric(args[5]) fileArray=args[6:length(args)] nFiles=length(fileArray) cat("found ",nFiles," files.\n") for (i in 1:nFiles) { arg<-fileArra...
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R
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require(GenomicSEM) library(data.table) NAME <- "MetS_firstorder" ldsc.out <- "../2_ldsc/ldscout/MetS_noUKB_ldscout.RData" sumstat.out <- "../7_sumstat/MetS_noUKB_sumstat.RData" load(ldsc.out) load(sumstat.out) model <- "F1 =~ 1*BMI_GIANT18 + WC_Watanabe_UKB F2 =~ 1*T2D_meta_FinR7_Mahajan22_MVP + FG_Lagou_M...
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R
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#library(glmnet::glmnet) library(testthat) n <- 1000 p <- 20 # set.seed(1000) X <- matrix(rnorm(n*p), n, p) beta <- rnorm(p) Xb <- X %*% beta y <- Xb + rnorm(n) * p y <- scale(y) * sqrt(n/(n-1)) X <- scale(X) * sqrt(n/(n-1)) r <- as.vector(t(X) %*% y) / n summary(r) summary(colSums(X^2)) summary(colSums(X)) summary(y)...
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R
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library(here) suppressPackageStartupMessages({ library(tidyverse) library(Biobase) library(ggpubr) require(janitor) }) # TIS weights file, with columns for GENE and Weights TIS_FILE <- here("ref/example_tis_weights_file.csv") # expression set of normalized gene counts with HGNC gene identifiers saved # as minimal ro...
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R
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#!/usr/bin/R # ---------------------------------------- # Write the merged + updated subtype UMAPs # Updated 01/07/2022 # ---------------------------------------- library(cbrbase) set_proj('DEVTRAJ', 'multiRegion') if (!exists("cellmeta")){ source(paste0(sbindir, 'load_metadata.R')) } # Create the C1/C2 (subtype ...
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R
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--- title: "Using pmlbr" --- ## Install The [**pmlbr** package](https://github.com/EpistasisLab/pmlb) provides the following user-facing functions and variables in the R environment. The package works for any recent version of R. You can install the released version of **pmlbr** from CRAN with: ```{r, eval=FALSE} i...
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R
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if (identical(.Platform$OS.type, "windows")) { test_that("compute environment executable tests require POSIX scripts", { skip("requires executable POSIX shell scripts") }) } else { make_fake_executable <- function(bin_dir, name, body = "echo fake") { path <- file.path(bin_dir, name) writeLines(c("#!/bin/sh...
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R
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library(pacman) p_load(tidyverse, lavaan, semTools, MplusAutomation, psych, ggplot2, sjlabelled, janitor, tidymodels, parsnip, dials, tune, finetune, xgboost, recipes, data.table, doParallel, vip, DALEX, modelDown, corrgram, hablar, SHAPforxgboost, ADNIMERGE, vetiver, pins, haven, magrittr, mirt, c...
ed931bd97327b6fbee0d1e2082b67de5f3d9ec92dbb3ebe27a787260e2c0e317
R
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library(pacman) p_load(tidyverse, lavaan, semTools, MplusAutomation, psych, ggplot2, sjlabelled, janitor, tidymodels, parsnip, dials, tune, finetune, xgboost, recipes, data.table, doParallel, vip, DALEX, modelDown, corrgram, hablar, SHAPforxgboost, ADNIMERGE, vetiver, pins, haven, magrittr, mirt, c...
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R
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options(bitmapType='cairo') args <- commandArgs(TRUE) name<-args[1] dataColumn<-as.numeric(args[2]) skip<-as.numeric(args[3]) minY<-as.numeric(args[4]) maxY<-as.numeric(args[5]) fileArray=args[6:length(args)] nFiles=length(fileArray) cat("found ",nFiles," files.\n") for (i in 1:nFiles) { arg<-fileArray[i] cat("\t"...
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R
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#!/usr/bin/R # -------------------------------------------------------- # Load data for the modules computed on external datasets: # Updated 12/01/2023 # -------------------------------------------------------- library(cbrbase) set_proj('DEVTRAJ', 'multiRegion') library(tidyr) # Arguments: args=(commandArgs(TRUE)) if...
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R
1,906
44
library("tidyverse") library("gprofiler2") library("data.table") library("BiocParallel") # load the DEG datasets setwd("./Projects/SNCSynuclein") # load the DEG files DEGFileCombind <- readRDS("Step8.SubCluster.DEG/DEG.random/DEGFileCombind.rds") Cluser <- names(DEGFileCombind) for (i in 1:length(Cluser)) tryCatc...
5a0afd004e6a7c9c3419bad81bf316bd368408ecbc25daf7f5f9a3ca5e106cd1
R
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library(lmerTest) # library(sjPlot) library(glmmTMB) library(ggplot2) library(dplyr) library(ggeffects) library(effects) library(readr) library(ggChernoff) library(merTools) library(lattice) library(stringr) # library(tidyverse) # ['moca','lns','hvlt','bjlot','sdmt','sft','pigd','tremor','seadl','updrs3','updrs2','updr...
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R
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# Commands to set up R 4.1.2 to use # module unload R/4.0.0 # module unload pcre2/10.35 # module load pcre2/10.39 # module load R/4.1.2 # Working off of a vignette at http://rstudio-pubs-static.s3.amazonaws.com/371411_b572e753d5e94c959cee7f05bc969839.html # for fitting a beta binomial mixed model using glm...
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R
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#Once you build your network with metacore see "Build Network using Metacore pdf" (building algorithm: direct interaction) you can run this script #get the xls file from Metacore for mapepd and interaction convert them to csv file and proceed with the script library(tidyverse) cells=c("Astrocyte","Oligodendrocyte...
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R
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library(tidyverse) library(ComplexHeatmap) library(colorRamp2) library(circlize) library(RColorBrewer) # Read in data rna_data <- read_csv("~/nepc_normalized_counts_vst.csv") # Transform to format needed to make heatmap, scale and center data rna_mat <- rna_data %>% column_to_rownames("gene") %>% t() rownames(rn...
a58dd7c61abe1d696a3cb3a336c9ae51305c76665b68575b2a4aca632b2691d8
R
1,911
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test_that("extract additional values from ggplot_build", { df <- data.frame( gp = rep(1:15, 2), y = rep(c(3,4), each=15), row.names = NULL) plot = ggpubr::ggline(df, x="gp", y="y", add="mean_se") result = data.frame( x = 1:15, y = 3.5, ymin= 3, row.names = NULL) expect_equal(extract_plot_data(plot, additiona...
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R
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### Ref links # http://tuxette.nathalievilla.org/?p=1696 # https://swcarpentry.github.io/r-novice-inflammation/05-cmdline/ ## ----------------------------------------------------------------------------- # get the input passed from the shell script. args <- commandArgs(TRUE) # test if there is at least one ar...
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R
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#!/usr/bin/env Rscript library(vioplot) args <- commandArgs(trailingOnly=TRUE) if (length(args) != 2) { stop("Usage: plot_variant_counts.R <variant_count_file> <caller>") } variant_count_file <- args[1] caller <- args[2] if (!file.exists(variant_count_file)) { stop(paste("File does not exist:", variant_count_fi...
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R
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library(spacexr) library(spASE) library(tibble) library(data.table) library(Matrix) library(dplyr) library(tidyr) cerebellum_ref <- readRDS('../inst/extdata/reference_scrna/cerebellum_sn_ref_spacexr.rds') PATH <- '../inst/extdata/visium/cerebellum_spatial' counts <- fread(file.path(PATH, 'tagged_bwt2_counts_nm3.csv')...
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library(spacexr) library(spASE) library(tibble) library(data.table) library(Matrix) library(dplyr) library(tidyr) cerebellum_ref <- readRDS('../inst/extdata/reference_scrna/cerebellum_sn_ref_spacexr.rds') PATH <- '../inst/extdata/visium/mixture' counts <- fread(file.path(PATH, 'tagged_bwt2_counts_nm3.csv')) positions...
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R
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--- title: "PeakCount Guide for NosaAnalysis" author: "Julia Thüringer" date: '`r Sys.Date()`' output: html_document: default pdf_document: null vignette: > %\VignetteIndexEntry{PeakCount Guide for NosaAnalysis} %\VignetteEngine{knitr::rmarkdown} \usepackage[utf8]{inputenc} --- ### Description ```{r descrip...
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R
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library("Seurat") library("tidyverse") setwd("./Projects/SNCSynuclein") # Intergrated datasets at the batch level Seurat.SNC.Ind <- readRDS("Step3.Clustering/Seurat.SNC.Ind.rds") ## Recluster with a higher resolution Seurat.SNC.Ind <- Seurat.SNC.Ind %>% FindClusters(resolution = 0.9) %>% identity() # visualiz...
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#### Nonnegative matrix factorization #### ## --------------------------------------- setMethod("NMF", "ANY", function(x, ncomp = 3, method = c("als", "mult"), verbose = getCardinalVerbose(), ...) { method <- match.arg(method) msg <- "estimating nonnegative matrix factorization " if ( method == "als" ) { .Log...
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R
1,931
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library(Seurat) library(DAseq) library(ggpubr) library(SingleCellExperiment) options(future.globals.maxSize = 4000 * 1024^20) Sys.setenv('R_MAX_VSIZE'=2500000000000) Oli<-readRDS("Oli.rds") Idents(Oli) <- "DA" Oli_DEG.DA<-FindMarkers(Oli, slot = "data", assay = "RNA", test.use = "MAST", ident.1 = "DA", ident.2 = "no...