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7346fd62a99a8460ad6fb6ff26046271932a07ea0b3e5ff93abde069d518f277
Perl
1,008
53
#!/usr/bin/env perl use strict; use warnings; my $usage = "\n\n\tusage: $0 graphFromIwormFasta.out\n\n"; my $file = $ARGV[0] or die $usage; main: { my %graph; open(my $fh, $file) or die $!; while (<$fh>) { chomp; my @x = split(/\t/); my $node_id = shift @x; my $spa...
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Perl
1,019
54
#!/usr/bin/env perl # # Author: Jue Ruan <ruanjue@gmail.com> # use Getopt::Std; use strict; our ($opt_h, $opt_t, $opt_s, $opt_m, $opt_x, $opt_p); getopts("ht:s:m:x:p:"); &usage if($opt_h); &usage(1) if(@ARGV < 2); my $ref = shift || &usage(1); my $rst = $ref; $rst=~s/\.fa$//; $rst=~s/\.fasta$//; my $ncpu = 4; $ncpu...
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Perl
1,022
51
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 fpkm.matrix pct_of_top_fpkm\n\n"; my $expr_matrix = $ARGV[0] or die $usage; my $pct_of_top = $ARGV[1] or die $usage; main: { open (my $fh, $expr_matrix) or die $!; my $header = <$fh>; chomp $header; $header =~ s/^\s+//; m...
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Perl
1,023
46
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 file.audit_summary\n\n"; my $filename = $ARGV[0] or die $usage; main: { my %yes_no_counter; my $total_reco = 0; my $total_ref = 0; my $total_FL = 0; open (my $fh, $filename) or die $!; while (<$fh>) { if (/ref_fa/...
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Perl
1,024
51
#!/usr/bin/perl -w # # Author: Ruan Jue # use strict; use Getopt::Std; my $prefix = $ENV{'PARAM_RENAME_FA_PREFIX'} || 'S'; my $suffix = $ENV{'PARAM_RENAME_FA_SUFFIX'} || ''; our ($opt_p, $opt_s, $opt_h, $opt_f, $opt_b, $opt_I); getopts("hp:s:f:b:I"); die("Usage: $0 [-p name_prefix] [-s name_suffix] [-f trans_file] [...
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Perl
1,030
44
#!/usr/bin/env perl use Getopt::Long; my $description; use Bio::SeqIO; use Bio::Seq; use File::Basename; my $format = 'fasta'; #my $chunksize = 2500; my $numchunks = 100; my $file = 0; my $pad = 0; GetOptions( "n:s"=>\$numchunks, "f:s"=>\$file, 'pad'=>\$pad ); my @suffixlist = qw(.gff .gff3 .gff2 .gtf .f...
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Perl
1,030
50
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use SAM_reader; use SAM_entry; my $usage = "usage: $0 file.sam\n\n"; my $sam_file = $ARGV[0] or die $usage; main: { my $sam_reader = new SAM_reader($sam_file); while (my $sam_entry = $sam_reader->g...
9e2ec998185c46fe42bbec3ad063a1d525c8c8f87c799557409bfade6bdeec33
Perl
1,043
55
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: all_diff_expr_results.txt min_abs_log_FC max_FDR\n\n"; my $dat_file = $ARGV[0] or die $usage; my $min_logFC = $ARGV[1] or die $usage; my $max_FDR = $ARGV[2] or die $usage; my %best; open (my $fh, $dat_file) or die $!; while (<$fh>) { my $line =...
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Perl
1,044
55
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 DE.graph\n\n"; my $DE_graph = $ARGV[0] or die $usage; =color_panel > colorpanel(10, 'black', 'purple', 'red') [1] "#000000" "#28083C" "#501078" "#7818B4" "#A020F0" "#A020F0" "#B818B4" [8] "#D01078" "#E7083C" "#FF0000" =cut my @colors = ( ...
dcbf95ddb3df3baa348a7fcdc95c94af0be2faaebdcee7f79c36061c99936214
Perl
1,049
61
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use SAM_reader; use SAM_entry; my $usage = "usage: $0 coordSorted.sam max_per_position\n\n"; my $sam_file = $ARGV[0] or die $usage; my $max_per_pos = $ARGV[1] or die $usage; main: { my $sam_reader = new SA...
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Perl
1,068
57
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 matrix.fpkm\n\n"; my $matrix = $ARGV[0] or die $usage; main: { open (my $fh, $matrix) or die "Error, cannot open file $matrix"; my $header = <$fh>; print $header; while (<$fh>) { chomp; my ($acc, @vals) = spl...
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Perl
1,071
53
#!/usr/bin/env perl use strict; use warnings; my $usage = "\n\n\tusage: $0 acc_list_file.txt target_db.fasta\n\n"; my $acc_list_file = $ARGV[0] or die $usage; my $target_db = $ARGV[1] or die $usage; main: { my $samtools = `sh -c "command -v samtools"`; unless ($samtools =~ /\w/) { die "Error, need...
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Perl
1,073
49
#!/usr/bin/env perl use strict; use warnings; my $usage = "\n\n\tusage: $0 scaff_pairs_A.txt scaff_pairs_B.txt ...\n\n"; my @pair_files = @ARGV; unless (scalar(@pair_files) > 1) { die $usage; } main: { my %top_pairs; foreach my $pair_file (@pair_files) { open (my $fh, $pair_file) or die "Error,...
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Perl
1,074
52
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 blastx.outfmt6 trinity.matrix\n\n"; my $blastx_file = $ARGV[0] or die $usage; my $trinity_matrix = $ARGV[1] or die $usage; main: { my %blastx_top_hit; # store for transcript and component { open (my $fh, $blastx_file) or die...
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Perl
1,090
48
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::Bin/../../PerlLib"); use Process_cmd; my $usage = "\n\n\tusage: $0 Trinity.fasta reads.fa [threads=1]\n\n"; my $trin_fa = $ARGV[0] or die $usage; my $reads_fa = $ARGV[1] or die $usage; my $CPU = $ARGV[2] || 1; main: { my $salmon...
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Perl
1,102
51
#!usr/bin/perl -w use strict; use warnings; unless(@ARGV==3){ die "USAGE: Using the MIS value and the common taxon, this programe calcualtes the stability of the network across timeline.\nperl $0 <MIS.lst> <common_tax.xls> <stabliity.xls>\!\n"; } my (%common_tax, %MIS_path); #Store the common taxo open IN, "$ARGV[1...
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Perl
1,104
54
#!/usr/bin/env perl use strict; use warnings; use FindBin; my $usage = "usage: $0 bfly.A.fasta [bfly.B.fasta ...]\n\n"; my @files = @ARGV or die $usage; main: { my %data; foreach my $file (@files) { open (my $fh, $file) or die "Error, cannot open file $file"; while (<$fh>) { ...
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Perl
1,106
44
#!/usr/bin/perl -w # # Author: Jue Ruan <ruanjue@gmail.com> # use strict; =pod Transform <dbg>.[1/2/3/frg/ctg].dot into GFA format. <dbg>.[1/2/3/frg/ctg].dot is the assembly graph from wtdbg TAG: gl: gap length rc: read count to support the link sq: sequence can be substr from <rd_name>_<FR>_<off>_<len>, F fwd, R r...
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Perl
1,127
64
#!/usr/bin/env perl use strict; use warnings; use Carp; use Data::Dumper; unless ($ARGV[0] && $ARGV[1]) { die "Usage: $0 \$fastaFile \$acc_listing\n"; } my $fastaFile = $ARGV[0]; my $acc_listing = $ARGV[1]; open (ACC, "$acc_listing"); my %accs; while (<ACC>) { chomp; my @x = split (/\s+/); foreach...
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Perl
1,134
78
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 venn.txt\n\n"; my $venn = $ARGV[0] or die $usage; my %class_to_att_count; my %atts; open (my $fh, $venn) or die "Error, cannot open file $venn"; while (<$fh>) { chomp; my ($feature, $class_list) = split(/\t/); my @classes = split(/,/, $class...
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Perl
1,146
53
#!/usr/bin/perl # miRDeep2 find-read-count perl script # Copyright (C) 2008 - 2011 Marc Friedländer # # This program is free software: you can redistribute it and/or modify # it under the terms of the GNU General Public License as published by # the Free Software Foundation, either version 3 of the License, or # (at ...
a6429d1d6e550693e327b7a6522fedeb0b604ab2f3e4a565c5e80aebe2db73f4
Perl
1,160
53
#!/usr/bin/env perl use strict; use warnings; use File::Basename; my $usage = "usage: $0 Trinity.fasta reads.{fa,fq}\n\n"; my $trinity_fasta = $ARGV[0] or die $usage; my $reads_fasta = $ARGV[1] or die $usage; main: { my $cmd = "fasta_file_header_stripper.pl < $trinity_fasta > $trinity_fasta.noheader"; &pr...
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Perl
1,163
56
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 file.intron_analysis ...\n\n"; my @files = @ARGV; unless (@files) { die "$usage\n\n"; } my @types = qw( NOVEL REF NOVELCOMBO REFCOMBO SUBrefCombo); print "#analysis\t...
e27220cacc911350174108dc6c05558398a67e77ce216d12729f5a91a84c6922
Perl
1,166
58
#!/usr/bin/env perl use strict; use warnings; my $usage = <<__EOUSAGE__; ############################################### # # Usage: $0 Trinity.fasta new_feature_id_mapping.txt # # The 'new_feature_id_mapping.txt' file has the format: # # current_identifier <tab> new_identifier # .... # # # Only those entri...
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Perl
1,168
55
#!/usr/bin/env perl use strict; use warnings; my $usage = "venn_pairwise_out.dat\n\n"; my $venn_txt = $ARGV[0] or die $usage; main: { my %combos; my %sample_pair_to_combo_count; open (my $fh, $venn_txt) or die $!; while (<$fh>) { chomp; my ($feature, $sampleA, $sampleB, $comb...
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Perl
1,169
71
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 file.maps\n"; my $file = $ARGV[0] or die $usage; my %genes; my %isoforms; my %gene_merges; my %isoform_merges; open (my $fh, $file) or die $!; while (<$fh>) { chomp; my @x = split(/\t/); my $genes = shift @x; my @entries = split(/,/, ...
b2cc70e1a1e657abc7bf9661b6580f7b8cc9dd66940389821bf1c8d13f0c20e0
Perl
1,175
48
#!/usr/bin/env perl use strict; use warnings; my $usage = "\n\n\tusage: $0 iworm.fasta iworm_cluster_welds_graph.txt\n\n"; my $iworm_fa = $ARGV[0] or die $usage; my $iworm_cluster_welds_file = $ARGV[1] or die $usage; main: { my %counter_to_iworm; { my $counter = 0; open(my $fh, ...
a9dce545dea7e06d046f9acc883a9ec6f4f5dd5d93d19045d9d8dfa80e360ff3
Perl
1,178
57
#!/usr/bin/perl # miRDeep2 remove-white-space-in-ID perl script # Copyright (C) 2009 - 2011 Sebastian Mackowiak # # This program is free software: you can redistribute it and/or modify # it under the terms of the GNU General Public License as published by # the Free Software Foundation, either version 3 of the Licens...
105eed51d4e28eded5931c1e038fb58864a6bde10459813bfbc382d8f3240260
Perl
1,184
62
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 file.frag_coords (LEND|REND)\n\n"; my $file = $ARGV[0] or die $usage; my $end = $ARGV[1] or die $usage; unless ($end eq "LEND" || $end eq "REND") { die $usage; } main: { my @histogram; my $curr_scaffold = ""; open (my $fh, $...
2bcf4d28a4dce9aac18ba2a3fdcac58651514a5c0383fcbc2d32475d9467df30
Perl
1,190
64
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fastq_reader; my $usage = "usage: $0 file.fastq\n\n"; my $fastq_file = $ARGV[0] or die $usage; main: { my $fastq_reader = new Fastq_reader($fastq_file); my $tab_tmp_file = "$fastq_file.tab"; o...
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Perl
1,193
59
#!usr/bin/perl -w use strict; use warnings; unless(@ARGV==3){ die "USAGE: This program helps to find the common taxo across the timeline.\nperl $0 <mean_abundance.lst> <AD or WT> <Common_tax.output>\!\n"; } my (%path, $target, %abun, %taxo); $target = $ARGV[1]; #To get the path for the mean_abundance.file open IN...
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Perl
1,197
64
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 roku.dat expr.dat\n\n"; my $roku_dat = $ARGV[0] or die $usage; my $expr_dat = $ARGV[1] or die $usage; my %acc_to_info; main: { { open (my $fh, $roku_dat) or die $!; while (<$fh>) { chomp; my @x = spli...
9395d50816cf82b0a6dba2ef8eb6fdd9cb653239494bb9623af5cfdac5b87061
Perl
1,203
52
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fastq_reader; my $usage = "\n\nusage: $0 interleaved.fq [left_output_filename.fq right_output_filename.fq]\n\n"; my $interleaved_fq = $ARGV[0] or die $usage; my $left_out_filename = $ARGV[1] || "unweaved.le...
3ab2283bbd583932cc9179954e4ee09979987e68d617134a52be46bbcd39f640
Perl
1,207
52
#!/usr/bin/env perl use strict; use warnings; use FindBin; my $usage = "usage: $0 genome.fasta left.fq right.fq [output_dir]\n\n"; my $genome_file = $ARGV[0] or die $usage; my $left_fq_file = $ARGV[1] or die $usage; my $right_fq_file = $ARGV[2] or die $usage; my $output_dir = $ARGV[3] || "bowtie.$$.dir"; while ...
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Perl
1,225
57
#!/usr/bin/env perl use strict; use warnings; ## Given a subset of the venn, extracts all entries from the original files containing the pairwise DE data. my $usage = "\n\nusage: $0 venn.selected pw1.summary pw2.summary ...\n\n"; my $selected_file = $ARGV[0] or die $usage; shift @ARGV; my @pw_summaries = @ARGV; u...
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Perl
1,230
74
#!/usr/bin/perl -w # #Author: Ruan Jue # use strict; use Getopt::Std; use DB_File; our ($opt_l, $opt_h); getopts("l:h"); my $level = $opt_l || 0; &usage if($opt_h); my $dbf = shift or &usage; if($dbf!~/\.dbm$/){ $dbf .= ".dbm" if(-e "$dbf.dbm"); } my @nodes = @ARGV; if(@nodes == 0){ while(<>){ chomp; push(@...
c8c735574ed30d0f69045582ad74446f6a030d539c5cc4661c249e8301b3bf86
Perl
1,239
52
#!/usr/bin/env perl # kj2 08.10.2020 use strict; use Getopt::Long; my $fa; my $printfa; my $split; my $help; GetOptions ( "fa:s" => \$fa, "printfa" => \$printfa, "h" => \$help, "help" => \$help, "split" => \$split, ); if (($help) || (!$fa)) { print "This script takes a fasta file and produ...
8758471ae68b785fb70073eb31e59eb028c932b07c9c08402dd8494539a592da
Perl
1,244
70
#!/usr/bin/env perl use strict; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use SAM_reader; use SAM_entry; my $usage = "usage: $0 nameSorted.sam\n\n"; my $sam_file = $ARGV[0] or die $usage; main: { my $sam_reader = new SAM_reader($sam_file); my $filtered_count = 0; my $total_coun...
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Perl
1,249
46
#!/usr/bin/perl -w use strict; die "Usage: perl $0 <ref> <kmer_length> <step_size> <output>\n" unless @ARGV == 4; my $storefile = &FastaReader($ARGV[0]) or die "fasta file is required!\n"; print STDERR "ref loading finished!\n"; my $length = $ARGV[1]; my $step = $ARGV[2]; open (OUT, ">$ARGV[3]") or die "permission deni...
70e9c4b02d546ad4a9001f3544f2c6831c2fd7c4be4aea20721979358ab0a03c
Perl
1,270
50
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib("$FindBin::Bin/../../PerlLib"); use Pipeliner; use Cwd; my $usage = "\n\n\tusage: $0 input.fasta reads.left.[fq|fa] reads.right.[fq|fa]\n\n"; my $target_fa = $ARGV[0] or die $usage; my $left_reads = $ARGV[1] or die $usage; my $right_reads = $ARGV[2...
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Perl
1,277
57
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 (count|percent) A.org_rep B.org_rep ...\n\n"; my $type = shift @ARGV; my @org_rep_files = @ARGV or die $usage; unless ($type eq 'count' || $type eq 'percent') { die $usage; } main: { my %matrix; my %species_to_total_percentage; ...
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Perl
1,286
74
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use WigParser; my $usage = "usage: $0 strand_coverage.wig strand[+-]\n\n"; # need strand value to include in the GFF file. my $wig_file = $ARGV[0] or die $usage; my $strand = $ARGV[1] or die $usage; main: { m...
d31de02ac2ece9e58d1d632020500e1e55130c93bae58591c94716d44d89bfca
Perl
1,301
57
#!/usr/bin/env perl use strict; use warnings; use lib($ENV{EUK_MODULES}); use Fasta_reader; my $usage = "\n\n\tusage: $0 trin_before.fa trin_after.fa\n\n"; my $trin_before_file = $ARGV[0] or die $usage; my $trin_after_file = $ARGV[1] or die $usage; main: { my %trin_seqs_before = &parse_trin_fa($trin_before_f...
22a485b0252429f8039b7152aee52373f21f3a5d1fc18beefe28e339bc452916
Perl
1,311
57
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fastq_reader; my $usage = "\n\nusage: $0 left.fq right.fq\n\n"; my $left_fq = $ARGV[0] or die $usage; my $right_fq = $ARGV[1] or die $usage; main: { my $left_fastq_reader = new Fastq_reader($left_fq); ...
ee33e8a5064a1a37a0b2ef7f3df4d06a3ecdca4f7a5c9a03e4b16cdf7e25dc46
Perl
1,314
55
#!/usr/bin/env perl use strict; use warnings; my $usage = "\n\n\tusage: $0 iworm_bundles_fasta_file sorted_reads_to_components_file\n\n\n"; my $iworm_bundles_fasta_file = $ARGV[0] or die $usage; my $reads_to_components_file = $ARGV[1] or die $usage; main: { my %component_to_LRs; { open (my $fh, $r...
2dced2d943596853eec0ca752a64e3964e253d708605d4be3487b47979b761d4
Perl
1,320
71
#!/usr/bin/env perl use strict; use warnings; use lib ($ENV{EUK_MODULES}); use Fasta_reader; my $usage = "usage: $0 transcripts.cdna.fasta\n\n"; my $transcripts_fasta = $ARGV[0] or die $usage; my $graphs_per_dir = 100; main: { my $fasta_reader = new Fasta_reader($transcripts_fasta) or die $!; ...
793818a97139d4b4988bb64835de345cfa104d3b5136e22a6fa07bfa872514b2
Perl
1,330
68
#!/usr/bin/env perl use strict; use warnings; my $usage = <<__EOUSAGE__; ############################################### # # Usage: $0 matrix.txt new_feature_id_mapping.txt # # The 'new_feature_id_mapping.txt' file has the format: # # current_identifier <tab> new_identifier # .... # # # Only those entries ...
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Perl
1,336
70
#!/usr/bin/env perl use strict; use warnings; my $usage = "\n\tusage: $0 A.FL_selected B.FL_selected [RESTRICT_TO_FAILURES]\n\n"; my $A_stats_file = $ARGV[0] or die $usage; my $B_stats_file = $ARGV[1] or die $usage; my $RESTRICT_TO_FAILURES = $ARGV[2] || 0; my $MIN_PER_LEN = 99; my $MAX_PER_GAP = 1; main: { ...
369f4ec9f393a9cd6b0186d402af2b3af318fda4d5ec4425ac85fc15680a96e4
Perl
1,337
61
#!/usr/bin/perl use strict; use warnings; use File::Basename; use Config::Simple; die "ERROR: Not enough argument.\nUSAGE: ./getChr15.pl <filedir> <ts>\n" if(@ARGV<2); my $dir = dirname(__FILE__); my $cfg = new Config::Simple($dir.'/app.config'); my $chr15dir = $cfg->param('data.chr15'); my $filedir = $ARGV[0]; $fil...
1505a1b111e62b466471af86803c96638709ed895cf63530665bc019d2753fac
Perl
1,346
70
#!/usr/bin/env perl use strict; use warnings; use lib ($ENV{EUK_MODULES}); use WigParser; use Gene_obj; my $usage = "usage: $0 clip.wig\n\n"; my $clip_wig = $ARGV[0] or die $usage; main: { my %mol_to_clips = &get_clip_pts($clip_wig); foreach my $mol (keys %mol_to_clips) { my @clip_...
b97beecfdd994c3f397bb7c09280f3a599c1f9a6cb20bfbc2d35f145b76a1dce
Perl
1,346
64
#!/usr/bin/env perl use strict; use warnings; my $usage = <<__EOUSAGE__; ############################################### # # Usage: $0 matrix.txt samples_with_relabeling.txt # # # where samples_with_relabeling.txt has the format: # # sample_name <tab> current_column_name <tab> new_column_name # .... # # On...
da4c33802900dbb50963994e56ae90278f3117d7d6fdea890b3f42d5d6aea7ae
Perl
1,379
68
#!/usr/bin/env perl use strict; use warnings; my $usage = "\n\tusage: $0 cd-hit.clstr\n\n" . "try running cd-hit first like so:\n" . "\tcd-hit-est -o cdhit -c 0.98 -i Trinity.fasta -p 1 -d 0 -b 3 -T 10\n\n"; my $cdhit_file = $ARGV[0] or die $usage; main: { my $num_bad_clusters = 0; my $cluster...
978c7d2d93f4baea88310df8aac3570014cee97cca584df3a9b06ffc510a6d5e
Perl
1,382
77
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use WigParser; my $usage = "usage: $0 wigA [ wigB ... ]\n\n"; my @wig_files = @ARGV; unless (@wig_files) { die $usage; } main: { my @data_arefs; my $max_pos = 0; foreach my $wig_file (@w...
8c39c518fd9248805e4eb81af1b001e3dd8813b9b88ca37c2666c2b2371d0d74
Perl
1,388
49
#!/usr/bin/env perl use strict; use warnings; use FindBin; ## we delete all files we don't need in this directory. Be careful in case users try running it somewhere else, outside this dir. chdir $FindBin::RealBin or die "error, cannot cd to $FindBin::RealBin"; `rm -rf ./trinity_out_dir*`; `rm -rf ./bowtie_out` ...
d90e7d2a8918a45d6c4d149e7565e3107f313cbd62af90ae0dd0da7bdaa813a3
Perl
1,410
47
#!/usr/bin/perl # miRDeep2 mirdeep-to-BED perl script # Copyright (C) 2009 - 2012 Sebastian Mackowiak # # This program is free software: you can redistribute it and/or modify # it under the terms of the GNU General Public License as published by # the Free Software Foundation, either version 3 of the License, or # (a...
f35cd3c50a0f8713e29d1c749e53ab78dd3d4d4571a37259216d928a8eac6ad1
Perl
1,410
53
#!/usr/bin/perl # miRDeep2 FASTQ-to-FASTA perl script # Copyright (C) 2008 - 2011 Marc Friedländer # # This program is free software: you can redistribute it and/or modify # it under the terms of the GNU General Public License as published by # the Free Software Foundation, either version 3 of the License, or # (at y...
d0aee9f679890672a9cdfa72afd38d79201189c1586bd7de69117dd27b42086f
Perl
1,413
70
#!/usr/bin/perl -w # #Author: Ruan Jue # use strict; use DB_File; my $dot_file = shift or die("Usage: $0 <dot_file>\n"); die("$dot_file.dbm already exists!!!") if(-e "$dot_file.dbm"); open(IN, "<", $dot_file) or die; my %hash; tie %hash, 'DB_File', "$dot_file.dbm", O_RDWR | O_CREAT, 0644, $DB_HASH or die "Cannot op...
0ce520780b9c4fd1887cdb8dbecbf95705a24ef90ef09f0ba1a70f334dedff4c
Perl
1,418
79
#!/usr/bin/perl ## support for gzip files use strict; my $id; my $seq; my @species = split(",",$ARGV[1]); die "Error: Specify species or comma separated list of species\n\nUsage:\n\tperl $0 miRBase_fasta_file species [mature/star]\n\te.g. $0 mature.fa cel mature\n\tfiles can be gzipped\t=>no reason to decompress th...
6b1a55a67897421942fa4de2e9cbe3b0e32b4e7f0319e08d8d7b8c28da04f15d
Perl
1,430
64
#!/usr/bin/env perl use strict; use warnings; my $usage = "\n\nusage: $0 all_diff_expression_results.txt\n\n"; my $results_summary_file = $ARGV[0] or die $usage; my @signif_results; { open (my $fh, $results_summary_file) or die "Error, cannot open file $results_summary_file"; while (<$fh>) { chomp; ...
b05df65ad3c45297b2934a3259bc222f175d5c911d59db1cef14eb86cbaa13e5
Perl
1,452
81
#!/usr/bin/perl # miRDeep2 Illumina-to-FASTA perl script # Copyright (C) 2008 - 2011 Marc Friedländer # # This program is free software: you can redistribute it and/or modify # it under the terms of the GNU General Public License as published by # the Free Software Foundation, either version 3 of the License, or # (a...
cf4300e5f24556e473c5dfaff6849f94deba5dcc746f007282cbfc546e2f1259
Perl
1,457
60
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fasta_reader; my $usage = "usage: $0 rsem.out blast.outfmt6 [transcripts.fasta]\n\n"; my $rsem_out = $ARGV[0] or die $usage; my $blast_out = $ARGV[1] or die $usage; my $transcripts_fasta = $ARGV[2]; main: { ...
364b9cc14ce5297465d8c11a3265cd7c55c99001a7f2a32169b36b5edd035a8e
Perl
1,460
71
#!/usr/bin/env perl use strict; use warnings; use Cwd; use FindBin; my $usage = "\n\nusage: $0 reads.fa hash_size\n\n"; my $reads_file = $ARGV[0] or die $usage; my $hash_size = $ARGV[1] or die $usage; my $JELLYFISH_DIR = $FindBin::RealBin . "/../../trinity-plugins/jellyfish-1.1.3"; my $CPU = 4; my $min_kmer_cov = ...
6f840bd57ad73f9d23997783c2cd62896adde7623bdf08d49d4c425d9ae9c8c4
Perl
1,467
56
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::Bin/../../PerlLib"); use Fasta_reader; my $usage = "usage: $0 chrysalis_component_listing.txt min_seq_length\n\n"; my $comp_list_file = $ARGV[0] or die $usage; my $min_seq_length = $ARGV[1] or die $usage; main: { my $ret_val = 0; ...
b01358fe94c9c5c9a374ffa2cee76fb0bb1df45df042e8daf2a7d628c5bdb142
Perl
1,468
55
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fastq_reader; my $usage = "usage: $0 left.fq right.fq\n\n"; my $left_fq = $ARGV[0] or die $usage; my $right_fq = $ARGV[1] or die $usage; main: { my $left_fq_reader = new Fastq_reader($left_fq); my $...
a1eda47e0b4dbad04b712e62583326eb65cffa56fea9778f9c32f505b25d4f5a
Perl
1,469
65
#!user/bin/perl -w use strict; use warnings; unless(@ARGV==3){ die "USAGE: This program calcuates the efficiency and EDR for the network without nodes.\nperl $0 <shortest.path.csv> <MIS.xls> <efficiency.xls>!\n"; } my(%nodes, $n); #Get the names of nodes open IN, "$ARGV[1]"||die $!; <IN>; while(<IN>){ chomp(my $li...
979e9931e86b43d11bdd343d5502a5fb898c4d15cba09b17182312de2e235c3b
Perl
1,485
78
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use WigParser; my $usage = "usage: $0 strand_coverage.wig min_coverage strand[+-]\n\n"; # need strand value to include in the GFF file. my $wig_file = $ARGV[0] or die $usage; my $min_coverage = $ARGV[1] or die $...
39f4882b02450eab6b411dadf606d5ed51da6aa0283d75a9c9954984e3eecbf8
Perl
1,495
77
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use SAM_reader; use SAM_entry; my $usage = "usage: $0 file.sam\n\n"; my $sam_file = $ARGV[0] or die $usage; main: { my $sam_reader = new SAM_reader($sam_file); while ($sam_reader->has_next()) { my $sam_en...
5164a9203312f6d090e79a78b070a3aba3c925f9704d71a388ba72763a63e5e8
Perl
1,500
67
#!/usr/bin/env perl use strict; use warnings; ## Just identifies and counts transcripts that are predicted to encode multiple ORFs # of those that encode an ORF of min length, how many encode multiple ORFs of min length? my $usage = "\n\nusage: $0 transdecoder.pep [min_prot_length=300]\n\n"; my $transdecoder_pep_f...
36a7eb152f0455cfe810adbfb928d47f99d6b01b5e5a2286b26ae67d848440c2
Perl
1,511
62
#!user/bin/perl -w use strict; use warnings; unless(@ARGV==4){ die "USAGE: This program calculates the MIS (abundance-weighted Mean Interaction Strength) for all nodes in the specific network.\nperl $0 <mean_abundece.csv> <correlation.matrix.csv> <AD or WT> <MIS.results>\!\n"; } chomp(my $target =$ARGV[2]); my (%ab...
e187d9cf9e306ab74a8316b120bdfe31284e503fae1888b8cb98cf4eedd518ec
Perl
1,528
65
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::Bin/../../PerlLib"); use Fasta_reader; use Process_cmd; my $usage = "\n\n\tusage: $0 Trinity.fasta [out_prefix='GC_content']\n\n"; my $fasta_file = $ARGV[0] or die $usage; my $out_prefix = $ARGV[1] || "GC_content"; main: { my $dat...
8ae9ca8f72325bed98206d65e7a4ce28828aee715b633c4568999e0283e91d1a
Perl
1,557
31
#!/usr/bin/env perl use Cwd qw(abs_path); use File::Basename; $bam = shift; #$bam =abs_path($bam); $ANNO='/home/weiqi/project/00.DATABASE/GRmm38/Annotation/gencode.vM24.annotation.bed'; $GENOME = '/home/weiqi/project/00.DATABASE/GRmm38/GRCm38.p6.fa'; $SIZE = '/home/weiqi/project/00.DATABASE/GRmm38/GRCm38.p6.fa.size';...
05399e7afddcbd182c21052145efeac65ab7929b59fd71e36eee08729ebe79b6
Perl
1,572
68
#!usr/bin/perl -w #use strict; use warnings; unless(@ARGV==3){ die "USAGE: This program evaluates the Robustness for a network through simulations of Random-Attack or Target-Removal.\nperl $0 <MIS.xls> <Hub.info> <Robustness.xls>\!\n\nThe format for the Hub.info file:\nFile Type\tPath to file\tRandom select number\n"...
a9390c2ec86b74c2e8518e16e201aa7417488d2440b6ae112b892a2b51a71d3f
Perl
1,578
77
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 inputFile out.cleanReads out.malformedReads\n\n"; my $inputFile = $ARGV[0] or die $usage; my $cleanReads = $ARGV[1] or die $usage; my $malformedReads = $ARGV[2] or die $usage; open (my $ofh_clean, ">$cleanReads") or die "Error, can't write to $cl...
a1118f8b115088c0bb4a3a8914a9cf90c0809becf98ba0c3f9ec4d14e8794bac
Perl
1,584
91
#!/usr/bin/env perl use strict; use warnings; use Carp; use Getopt::Long qw(:config posix_default no_ignore_case bundling pass_through); use List::Util qw(shuffle); my $usage = <<__EOUSAGE__; ###################################################################################### # # Required: # # --cmds <string> ...
555f97be3121ec6aeb93433f6230f61d29efcb1b2a1d35430377c091e0a37256
Perl
1,603
65
#!/usr/bin/perl -w use strict; use Data::Dumper; die "Usage:perl $0 <gff|gff.gz> <Mammalia.gene_info> <tax id> <annovar result> <out>\n" unless @ARGV == 5; my $gff = $ARGV[0]; open (GFF,($gff =~ /\.gz$/)? "gzip -dc $gff |" : $gff) or die "gzipped gff file required!\n"; open (OUT, ">$ARGV[4]") or die "permission denied!...
733e09ba18ef94d348c322d00a8242b2f746e2ecba0ec28512a13c0581643faa
Perl
1,609
70
#!/usr/bin/perl use strict; use warnings; #use autodie ':all'; # use Compress::Zlib <- don't use this - too slow use Time::HiRes 'time'; use Getopt::Std ; our ($opt_h, $opt_f,$opt_q, $opt_v, $opt_t); getopts("hvqf:t:"); &usage if $opt_h; sub usage { die "FindMethylatedCoordinatesForAllStrainsInOneTissue.pl -f <...
b9c79906d106dd6a33626c1d61b7b395558b362b13a0fb48bfb1712a659a8d87
Perl
1,644
95
#!/usr/bin/env perl use strict; use warnings; use FindBin; use File::Basename; use Cwd; use Carp; use Getopt::Long qw(:config no_ignore_case bundling pass_through); my $usage = <<__EOUSAGE__; ###################################################################### # # Required: # --genome <string> targe...
6fdcc0a6bfe7a43cc6904241e7cb82edc70d1b796546410ef18aef0c8e04261a
Perl
1,662
61
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 flag_number\n\n"; my $flag = $ARGV[0] or die $usage; my @tokens; my $pair_flag = ($flag & 0x0001) ? "PAIRED" : "unpaired"; push (@tokens, $pair_flag); my $query_unmapped_flag = ($flag & 0x0004) ? "query_unmapped" : "QUERY_MAPPED"; push (@token...
40f1e6141aae27b497f851e5a702d5e5b585f0ebeb5b63fe3f1db98f05099ae4
Perl
1,668
84
#!/usr/bin/env perl use strict; use warnings; use File::Basename; use Getopt::Long qw(:config no_ignore_case bundling pass_through); my $usage = "\n\nusage: $0 --prefix \$prefix fileA.sra [fileB.sra ...]\n\n\n"; my $prefix; &GetOptions ('prefix=s' => \$prefix); my @sra_files = @ARGV; unless (@ARGV) { die $...
91bd18a79e1de466f25b8fca4a5b3184a20290352fd3e73d8670cac488e60091
Perl
1,675
83
#!/usr/bin/env perl use strict; use warnings; use FindBin; use Getopt::Long qw(:config no_ignore_case bundling pass_through); my $usage = <<__EOUSAGE__; #################################################################################### # # usage: $0 --reads_list_file <string> --out_token <string> [Trinity params...
472409c326d325a78c77df290961168955b7497134577bb7d60f492c01d85026
Perl
1,689
62
use marisa; $keyset = new marisa::Keyset; $keyset->push_back("cake"); $keyset->push_back("cookie"); $keyset->push_back("ice"); $keyset->push_back("ice-cream"); $trie = new marisa::Trie; $trie->build($keyset); print("no. keys: ", $trie->num_keys(), "\n"); print("no. tries: ", $trie->num_tries(), "\n"); print("no. node...
d16f034df533e0ad21c78a4c8cf7058b646cb92b1705fd69ee3705ebeba1b0b5
Perl
1,708
78
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib", "$FindBin::RealBin/../../PerlLib/KmerGraphLib"); use Fasta_reader; use StringGraph; use StringNode; use ColorGradient; my $usage = "usage: $0 Butterfly.fasta\n\n"; my $butterfly_fasta_file = $ARGV[0] or die $usag...
0f25bd4f2a1b441444ab3cd231d9e513e271722bad2eb7fd014d46c2f6b37434
Perl
1,717
82
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 annot.gff3 target_file.txt\n\n"; my $annot_gff3 = $ARGV[0] or die $usage; my $target_file = $ARGV[1] or die $usage; main: { my %trans_id_to_annot = &parse_trans_names($annot_gff3); open (my $fh, $target_file) or die "Error, cannot open ...
827c24b7485eb4345a43d6c790ea85e86ba2d6c03749c3ca3cbee867fe15853b
Perl
1,723
84
#!/usr/bin/env perl use strict; use warnings; my $usage = "\n\nusage: $0 RSEM.genes.results\n\n"; my $rsem_file = $ARGV[0] or die $usage; main: { my @entries; open (my $fh, $rsem_file) or die $!; my $header = <$fh>; my $max_fpkm = 0; while (<$fh>) { chomp; my @x = split(/\t/);...
9641392078f883b77aab7fb73623b5d4ef2390adb660b4c7a5120e33af00d3c1
Perl
1,726
74
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fasta_reader; my $usage = "usage: $0 file.fasta [require_proper_pairs_flag] [include_volcano_spread]\n\n"; my $fasta_file = $ARGV[0] or die $usage; my $require_proper_pairs_flag = $ARGV[1] or die $usage; my ...
dc6002a25e9f1bd8c137f94f240c1ca32a8db132216e13997e88f4e05beb2d1e
Perl
1,726
82
#!/usr/bin/env perl use strict; use warnings; ## sums up reads per gene, but uses the single isoform with the highest sum counts as the representative entry in the reported 'gene' matrix. my $usage = "usage: $0 trinity_trans_matrix > trinity_rep_trans_matrix\n\n"; my $trans_matrix_file = $ARGV[0] or die $usage; m...
6533d50ccec63c8638a45cba7f9aa96301b113bc63427174c5913cd13387c248
Perl
1,737
58
#!/usr/bin/perl use strict; use warnings; use Vcf; die "ERROR: not enough arguments\nUSAGE: ./exacext.pl <filedir>\n" if(@ARGV < 1); my $filedir = $ARGV[0]; #local my $file = "/media/sf_Documents/VU/Data/ExAC0.3/ExAC.r0.3.sites.vep.vcf.gz"; #local my $file = "/data/ExAC/ExAC.r0.3.sites.vep.vcf.gz"; my $in = $filedir....
9d94fefc813d2cb8eaa04d31dfde92397ac59ba8376b1749f4f7d63b1f846fdf
Perl
1,738
85
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use SAM_reader; use SAM_entry; use Nuc_translator; my $usage = "usage: $0 file.sam out_prefix\n\n"; my $sam_file = $ARGV[0] or die $usage; my $out_prefix = $ARGV[1] or die $usage; main: { my $sam_reader...
286ba234a2e1f9c81d11b5f7f584b4ca0cd6e90230f00d3bda7004e985fb84b2
Perl
1,746
62
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fasta_reader; print STDERR "\n\n\tNOTE - longest transcript isn't always the best transcript!... consider filtering based on relative expression support ... \n\n"; my $usage = "usage: $0 Trinity.fasta\n\n"; m...
c05e22fd445abe873c718641175f80f63d81528a2972d1f4caa617cd19ade2c1
Perl
1,770
85
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../../PerlLib"); use Nuc_translator; my $usage = "usage: $0 blat.psl.nameSorted.sam reads.tab.nameSorted\n\n"; my $sam = $ARGV[0] or die $usage; my $seqs = $ARGV[1] or die $usage; main: { open (my $sam_fh, "$sam") or die...
787b487821d87d2c9a015d6c34d814f4d9c226ccc06ee648c85f0e2b5167d5c4
Perl
1,779
66
#!/usr/bin/perl # miRDeep2 convert-bowtie-output perl script # Copyright (C) 2009 - 2011 Sebastian Mackowiak # # This program is free software: you can redistribute it and/or modify # it under the terms of the GNU General Public License as published by # the Free Software Foundation, either version 3 of the License, ...
fc3d77c9740b531a16dc0918678fced6b7ef0e759fbd66368cf3695bff5462a7
Perl
1,783
69
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 summary_file num_top_ranks\n\n"; my $summary_file = $ARGV[0] or die $usage; my $num_top_ranks = $ARGV[1] or die $usage; main: { my %method_pairs; open (my $fh, $summary_file) or die $!; while (<$fh>) { chomp; my ...
7c7d1e44dc6411ed97df8b895950bfebd3307fb8b337bd9add8dac83e7eaa9ce
Perl
1,794
98
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fasta_reader; my $usage = "usage: $0 file.fasta [kmer_length=25]\n\n"; my $fasta_file = $ARGV[0] or die $usage; my $kmer_size = $ARGV[1] || 25; main: { my $fasta_reader = new Fasta_reader($fasta_file);...
7b3724a1d3eec3409e9727918277574dc9562fefba4c178a903c9bb5602aaf01
Perl
1,798
80
#!/usr/bin/env perl use strict; use warnings; no strict 'subs'; #use BSD::Resource; main: { print "\n\nBefore unlimit stack space:"; system("bash -c ulimit -a"); print "Attempting to unlimit stack space..."; try_unlimit(); print "After unlimit stack space: "; system("bash -c u...
b33d422f09283eda6ce4b6f92e348fcfd0bc8279e326a7765e8cde0d87b189bb
Perl
1,836
69
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::Bin/../../PerlLib"); use Fasta_reader; use List::Util qw(min max); my $usage = "\n\n\tusage: $0 blast.outfmt6 query_fasta target_fasta\n"; my $blast_file = $ARGV[0] or die $usage; my $query_fasta = $ARGV[1] or die $usage; my $target_fasta...
0aad3029fd7247c57149dca4cb7238689df28197b4c7587e1bd27f9f6fa46dc9
Perl
1,842
84
#!/usr/bin/perl # miRDeep2 GEO-to-FASTA perl script # Copyright (C) 2008 - 2011 Marc Friedländer # # This program is free software: you can redistribute it and/or modify # it under the terms of the GNU General Public License as published by # the Free Software Foundation, either version 3 of the License, or # (at you...
6c49a790c439d0382f7e8e7501722a5d36ba3f47e79e64c5e4a26e50d0f6468f
Perl
1,855
95
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Gene_obj; my $usage = "usage: $0 transcripts.gtf\n\n"; my $gtf_file = $ARGV[0] or die $usage; main: { my %genome_trans_to_coords; open (my $fh, $gtf_file) or die "Error, cannot open file $gtf_file"; ...
1e6cdc0264392fc48bcb58766b948c02be234992a4a1f63253213b17be239017
Perl
1,858
103
#!/usr/bin/env perl use strict; use warnings; use FindBin; use File::Basename; use Cwd; use Carp; use Getopt::Long qw(:config no_ignore_case bundling); my $usage = <<_EOUSAGE_; ################################################################## # # Required: # # --target <string> : transcript sequences (eg. ...
dc928711866be25e2e62474c38b9569f90c76074f7bd312fa38524b7a7cd5238
Perl
1,861
69
#!/usr/bin/env perl use strict; use warnings; my $usage = "usage: $0 MAX_FDR MIN_FC\n\n" . " \n\tRun this in the edgeR results directory.\n\n"; my $MAX_FDR = $ARGV[0] or die $usage; my $MIN_FC = $ARGV[1] or die $usage; my $MIN_LOGFC = log($MIN_FC)/log(2); main: { my @DE_files = <*.DE_results>; my...
88ce0a3ec6afdd6572d93e42824e5b5fcc6c65487c285b0636c6dd66e4147736
Perl
1,870
76
#!/usr/bin/env perl use strict; use warnings; use FindBin; use lib ("$FindBin::RealBin/../../PerlLib"); use Fasta_reader; my $usage = "usage: $0 gmap.gff3 transcripts.fasta\n\n"; my $gmap_gff3 = $ARGV[0] or die $usage; my $trans_fa = $ARGV[1] or die $usage; main: { my %trans_coords_matched; open (my $fh...