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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') .getYesNo<-function(DType,CType){ DType=DType CType=CType i.yes=c() j.yes=c() i.no=c() j.no=c() ...
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#Extract cells from Ctrl and treatment condition for StemID library(RaceID) library(RColorBrewer) # load preprocessed RData object: sc_aMI_2 sc_aMI_2 = readRDS("sc_AMI.RData") # subset DMSO treated cells DMSO_aMI<- sc_aMI_2@expdata[, grep('^aMIDMSO', colnames(sc_aMI_2@expdata))] DMSO_aMI <- SCseq(DMSO_aMI) gm <- rown...
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library(RCTD) library(Matrix) input_dir = '/home/kd/Desktop/porownanie_cable_magisterka/final/preprocessed' dirs = dir(input_dir) # sciezka do folderu z podfolderami danych # reference_moje <- dgeToSeurat('/home/kd/Desktop/porownanie/Vignette_SC_wlasne') #SC dowolne, byle sie zgadzamly na...
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--- title: "Stats. for Figure 10" output: html_notebook --- ```{r message=FALSE, warning=FALSE, echo=FALSE,include=FALSE} rm(list = ls()) library(lme4) library(lmerTest) library(emmeans) library(dplyr) library(ggpubr) library(ggplot2) library(rstatix) library(effsize) library(magrittr) library(knitr)...
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# # # # # # # # # # # # # # # # # # # # # # # # # # SUPREME- Similarity Network Generation Part # # # # # # # # # # # # # # # # # # # # # # # # # # require(cluster) set.seed(404) topx = 10 # number of edges to keep # If the similarity metrics are same, then it will include all the edges with that value, t...
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library(Seurat) library(ggplot2) library(miloR) library(readxl) library(gridExtra) library(cowplot) setwd("/data/aronow/Kang/single_cell_projects/10X-Pak/integration/patient_derived_engineered/v2_filtered/") organoids = readRDS("./combined_all_organoids_filtered_v2.rds") df_cells = read.table("ctype_v6.txt", sep = "\...
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shell_call <- function(command, ...) { result <- system(command, intern = TRUE, ...) cat(paste0(result, collapse = "\n"))} shell_call("sudo apt install libgsl-dev") shell_call("sudo apt-get install libgmp3-dev") shell_call("pip install igraph") shell_call("pip install leidenalg") install.packages('quadprog') shell_...
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## Summarizes data, handling within-subjects variables by removing inter-subject variability. ## It will still work if there are no within-S variables. ## Gives count, un-normed mean, normed mean (with same between-group mean), ## standard deviation, standard error of the mean, and confidence interval. ## If there ar...
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# Plot best fitting Omega for different learning models in difficult trials library(tidyverse) library(patchwork) library(ggpubr) library(rstatix) load("processed_data/Results/df_LLhood_absolute.Rdata") # Create subdataframe with difficult trials only df_Omega <- dfabs_LLhood |> filter(level == "difficult") |> ...
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#From Liam Revell's web page #http://blog.phytools.org/2012/01/function-to-get-descendant-node-numbers.html library(phytools) getDescendants<-function(tree,node,curr=NULL){ #plot(tree,font=0.25); nodelabels(bg="white") if(is.null(curr)) curr<-vector() daughters<-tree$edge[which(tree$edge[,1]==node),2] curr<-c...
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#/home/toolkit/tools/R4.2.0/bin/Rscript #/home/toolkit/tools/R4.2.0/bin/R source('https://gitee.com/jumphone/VISA/raw/master/VISA.R') mat=read.table('mat.txt', sep='\t', row.names=1, header=TRUE,check.names=FALSE) a=t(mat)[,1] b=t(mat)[,2] setwd('/home/disk/database/data/ICS_scHIC/f00_demo') CEX=1 PCH=15 COL_LAB...
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# Load packages library(DOSE) library(clusterProfiler) library(org.Mm.eg.db) library(enrichplot) # GO analysis using genes downregulated in TTS compared to BP only in DMSO condition # Load data file named Decreased_In_C3_comp_IP_DMSO_electroporated.csv for this analysis symbols_1 <- as.character(read.csv2("Decreased...
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#/home/toolkit/tools/R4.2.0/bin/R library(Seurat) library(Signac) source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') library(data.table) library(stringr) pbmc=readRDS('./eQTL/scAtlas_pbmc.rds') SNP_TSS=read.table('./eQTL/SNP_TSS_500k.bed.loop.txt',sep='\t',row.names=NULL,header=FALSE) SNP_TSS_TAG=SNP_TSS[...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') .getYesNo<-function(DType,CType){ DType=DType CType=CType i.yes=c() j.yes=c() i.no=c() j.no=c() ...
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context("Test .search_tilt_perc") test_that(".search_tilt_perc works", { # load("tests/assets/test_data1.RData") load("../assets/test_data1.RData") averaging_mat <- test_data$averaging_mat score_1 <- test_data$score_1 score_2 <- test_data$score_2 svd_1 <- test_data$svd_1 svd_2 <- test_data$svd_2 target...
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# Utilities for PACNet webApp only #' network influence score #' #' Computes network influence score (NIS). #' @param expDat ranked query expression matrix, output of running logRank() #' @param stQuery query sample metadata table #' @param iGenes character vector of gene names found in both training data and query da...
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#' Check data object #' #' We choose to summarize the clustered gene expression data and metadata in a data object of #' the \code{\link[SingleCellExperiment]{SingleCellExperiment}} class. #' This function helps to check if the data object is compatible with \code{\link[FRmatch]{FRmatch}}. #' #' @param sce.object Data...
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#' Fetch PubMed records by PMID #' #' It downloads metadata for a set of PubMed articles identified by their PMID (PubMed Identifier). #' This is useful for retrieving specific known articles, updating existing datasets, #' or downloading records identified through other sources. #' #' @param pmids is a character or nu...
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--- title: "SiT - Figures" author: '[Kevin Lebrigand](mailto:lebrigand@ipmc.cnrs.fr)§, Joseph Bergenstråhle §, Kim Thrane §, Annelie Mollbrink, Pascal Barbry, Rainer Waldmann and Joakim Lundeberg' date: 'Compiled: `r format(Sys.Date(), "%B %d, %Y")`' abstract: 'The spatial landscape of gene expression isoforms in tissu...
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#' Normalization function for FR-Match analysis. #' #' @param sce.object Data object. #' @param scale Boolean variable indicating if to scale the PER GENE expression to the range [0,1]. Defualt: \code{TRUE}. #' @param norm.by Normalization options PER CLUSTER. Choose from \code{"softmax", "mean", "median", "none"}. #'...
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#This script takes the CNR and Euler number output from #cnr_euler_number_calculation.sh #for Freesurfer processing and calculates binary flags for the euler number and cnr subcategories #adopted from https://github.com/PennBBL/conte/tree/master/freesurfer ## modified by Hyun Joo Yoo, hyunjooy@usc.edu, 08/22/2020 ##...
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--- title: "Stats. for Figure S12" output: html_notebook --- # Libraries ```{r message=FALSE, warning=FALSE, echo=FALSE,include=FALSE} rm(list = ls()) library(lme4) library(lmerTest) library(emmeans) library(dplyr) library(ggpubr) library(ggplot2) library(rstatix) library(effsize) library(magrittr) ...
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library(lme4) library(MuMIn) library(lmerTest) changeDirection <- function(df,form){ colnumber=which(colnames(df)=='DistanceBin') for (sub in unique(df$ID)){ for (hemi in unique(df$Hemi)){ for (cond in c('C','I')){ mask=df$ID==sub & df$Cond==cond & df$Hemi==hemi if (sum(mask)>0){ ...
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# library(cowsay) #https://onlineasciitools.com/convert-text-to-ascii-art # writeLines(capture.output(say("Hello"), type = "message"), con = "ascii_art.txt") # art <- readLines("logo.txt") # dput(art) #' # metnormalizer_logo <- # c("", " _ __ _ _ ", # "...
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# genes genes_mature = c('STMN2', 'GAP43', 'DCX') genes_progenitor = c('VIM', 'HES1', 'SOX2') genes_cycle = c('CCNB2', 'CDK1', 'MKI67') genes_npc = c('PTTG1', 'PCNA', 'HMGB2', 'MKI67') genes_choroid = c('TPBG') genes_mesenchymal = c('DCN') genes_microglia = c('CD74') genes_glia = c('HOPX', 'FAM107A', 'TNC', 'AQP4', 'S...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') COL_LIST=list(DType=c('scATAC'='indianred1','scHIC'='royalblue1'), CType=c('Astro'='red','MG'='gold1','Neuron....
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make.slouch.simmap.table<-function(simmap.output,num.it){ #,model.type,pred.type,num.sp,prev.output=NULL){ #Code to create or update a table from slouch output #Format for calling function: make.slouch.table(Slouch output,type of model,type of p...
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--- title: "Figure S13" output: html_notebook --- # Libraries ```{r message=FALSE, warning=FALSE, echo=FALSE,include=FALSE} rm(list = ls()) library(dplyr) library(ggpubr) library(ggplot2) library(rstatix) library(magrittr) library(knitr) library(openxlsx) ``` # Read Data ```{r} df.all <...
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# Data preprocessing for GLM Results # IMPORTANT: It is absolutely necessary to start a new R Session before # running this script! # .rs.restartR() # Purpose: For plotting! library(fs) library(tidyverse) ### === LOAD DATA === ### file_list <- dir_ls("processed_data/GLM") # Load all data at once lapply(file_list...
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# RPCA (fast integration using reciprocal PCA) after removal of 9 low quality clusters library(Seurat) library(ggplot2) library(patchwork) library(dplyr) library(SeuratWrappers) library(monocle3) library(harmony) options(future.globals.maxSize=(90*1024^3)) # set max memory size as 60G setwd("/data/aronow/Kang/single_c...
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--- title: "AspectRation" author: "Gianfranco" date: "10/23/2021" geometry: margin=1cm output: pdf_document: default html_document: df_print: paged --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) library(lme4) library(MuMIn) library(lmerTest) library(data.table) setFactors <- function(df){ ...
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prepare_matrix_B <- function(ST_data, address_candidate, cell_types, lead_genes, tresh_tau, tresh_rho ){ nTypes <- length(cell_types) list_marker_genes_all_types <- vector("list", length = nTypes) for(i in 1:nTypes){ print(i) candidate_markers <- unique(unlist(read.table(paste0(address_candidate, cell_typ...
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library(Seurat) library(SeuratWrappers) library(monocle3) library(ggplot2) library(ggridges) source('../functions/_global.R') source('../functions/_visualize.R') # load data for integrated organoid samples donor = readRDS('../../donor_reintegrate/donor_reintegrated.rds') donor = donor[, donor$cellclass_draft_v2 %in% ...
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suppressMessages(library(tidyverse)) suppressMessages(library(lightgbm)) suppressMessages(library(optparse)) suppressMessages(library(fs)) #Parse arguments option_list <- list( make_option(c("-p", "--path"), type = "character", default = NULL, help = "Location of BoostMEC model objects"), make_option(c("-d", "--da...
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### Create sample distribution plots & linear age regression ### library("readr") library("plyr") library("dplyr") library("ggplot2") # Meta data for whole chimpanzee sample # chimp.meta <- read.csv("Chimp_meta_new_223.csv") # Meta data and whole brain volumes for # # GM, WM, & CSF for QC passed chimp...
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## Slingshot for pseudotime analysis # Load packages library(BiocStyle) library(readr) library(data.table) library(dplyr) library(tools) library(SingleCellExperiment) library(HDF5Array) library(mvoutlier) library(scran) library(scater) library(SC3) library(slingshot) library(stringr) library(org.Mm.eg.db) library(m...
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## given 2 vectors, find the hyperplane 2D representation .representation_2d <- function(vec1, vec2, tol = 1e-6){ stopifnot(length(vec1) == length(vec2), .l2norm(vec1) > tol, .l2norm(vec2) > tol) unit1 <- vec1/.l2norm(vec1) tmp <- .project_vec2vec(vec2, unit1, orthogonal = T) if(.l2norm(tmp) < tol){ retu...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') .getYesNo<-function(DType,CType){ DType=DType CType=CType i.yes=c() j.yes=c() i.no=c() j.no=c() ...
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#Generate 10% missing values at Random NeuroIm1.mis <- prodNA(NeuroIm1, noNA = 0.1) #Check missing values introduced in the data summary(NeuroIm1.mis) # mice_plot <- aggr(NeuroIm1.mis, col=c('navyblue','yellow'), # numbers=TRUE, sortVars=TRUE, # labels=names(NeuroIm1.mis), cex.axis=....
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# Quantify orthogonal predictions between models # df = data from response-based model # df_generative = data from stimulus-based model # Transition Probability CountRatio_TP <- function(df, df_generative) { # Compute response-based correct prediction respcorrect <- tibble(df$response, ...
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suppressMessages(library(tidyverse)) suppressMessages(library(lightgbm)) suppressMessages(library(optparse)) suppressMessages(library(fs)) #Parse arguments option_list <- list( make_option(c("-p", "--path"), type = "character", default = NULL, help = "Location of BoostMEC model objects"), make_option(c("-d", "--da...
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--- title: "AF22_DEU" author: "Marika Oksanen" date: '2022-06-17' output: html_document --- ```{r} library(DEXSeq) library(biomaRt) library(VennDiagram) library(tidyverse) ``` ## Preparations Flattened annotation file was created with python script dexseq_prepare_annotation.py -r was set to no --> shared exons are e...
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#' Plot cell enrichment #' #' @param cell_enrichment_res the result of \code{tiltedCCA::postprocess_cell_enrichment} #' @param col_palette vector of colors (as strings), equal to the number of celltypes in \code{cell_enrichment_res} #' @param cex_axis positive number (graphical parameter) #' @param cex_lab positive nu...
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#' @title peak_plot #' @description peak_plot #' @author Xiaotao Shen #' \email{shenxt@@sioc.ac.cn} #' @param index index #' @param sample sample. #' @param sample.nor sample.nor. #' @param QC QC #' @param QC.nor QC.nor #' @param tags tags #' @param sample.order sample.order #' @param QC.order QC.order #' @param tags t...
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library(Seurat) library(SeuratWrappers) library(monocle3) library(ggplot2) library(ggridges) library(dplyr) cds = readRDS("./cds_monocle3.rds") df_cells = read.table("../ctype_v6.txt", sep = "\t", header = 1, row.names = 1)[colnames(cds),] for (col in colnames(df_cells)){ if (!col %in% colnames(cds@colData)){cds@col...
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## VERY IMPORTANT -- SCRIPT 1 [FOR THE PIPELINE WORKFLOW] #!/usr/bin/env Rscript args = commandArgs(trailingOnly=TRUE) print(args) arg_file = as.array(args[1]) #print(arg_file) #j_global=as.numeric(args[2]) dataset_file = as.array(args[2]) #workspace_directory=as.array(args[4]) workspace_directory=as.array(args[3]) #i_...
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# Correlate gene expression between clusters source("./src/seurat_utils.R") for (cell_type in c( "GABA", "Glut")){ print(paste("Comparing cell type", cell_type)) print("Loading bird data") bird <- LoadH5Seurat("./data/seurat/bird.h5seurat") bird # 218342 features across 29497 samples within 1 assay # Selec...
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context("Test svd") ## .svd_safe is correct test_that(".svd_safe works", { set.seed(10) mat <- MASS::mvrnorm(n = 100, mu = rep(0, 10), Sigma = diag(10)) rownames(mat) <- paste0("c", 1:100) colnames(mat) <- paste0("g", 1:10) res <- .svd_safe(mat = mat, check_stability = T, ...
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library(ggplot2) library(Seurat) # load data setwd("/data/aronow/Kang/single_cell_projects/10X-Pak/integration/patient_derived_engineered/v2_filtered/visualize/") organoids = readRDS("../combined_all_organoids_filtered_v2.rds") df_cells = read.table("../ctype_v6.txt", sep = "\t", header = 1, row.names = 1) df_cells = ...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') COL_LIST=list(DType=c('scATAC'='indianred1','scHIC'='royalblue1'), CType=c('B'='blue3','Mono'='gold1','T'=...
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--- title: "Heatmaps" author: "Bismark Appiah" date: "14 December 2021" output: pdf_document: default html_document: default --- ```{r, setup, include=FALSE} # Set root directory for markdown file knitr::opts_knit$set(root.dir = 'your_directory_here') knitr::opts_chunk$set(echo = TRUE) ``` # 1. R...
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#' Horizontal Study Effects with Confidence Intervals #' #' Builds a custom version of \code{\link[ggstance]{geom_pointrangeh}}. #' #' @inheritParams ggplot2::layer #' @inheritParams ggstance::geom_pointrangeh #' @author Ilari Scheinin #' @export #' @examples #' library(ggplot2) #' library(magrittr) #' df <- #' # Use...
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library(Seurat) library(dplyr) library(pheatmap) library(tidyverse) library(ggplot2) library(RColorBrewer) library(knitr) library(xtable) library(ggrepel) library(cowplot) library(metap) library(formattable) library(STutility) library(Nebulosa) library(DoubletFinder) library(slingshot) library(formattable) library(Enha...
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library(Seurat) library(SeuratWrappers) library(monocle3) library(ggplot2) source('../../v2_filtered_new/Figures/functions/_global.R') source('../../v2_filtered_new/Figures/functions/_visualize.R') combined_organoid = readRDS("../combined_all_organoids_filtered.rds") cds = as.cell_data_set(combined_organoid, assay = ...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') .getYesNo<-function(DType,CType){ DType=DType CType=CType i.yes=c() j.yes=c() i.no=c() j.no=c() ...
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library(ggplot2) library(Seurat) setwd("/data/aronow/Kang/single_cell_projects/10X-Pak/integration/patient_derived_engineered/v2_filtered/") # load data df_cells = read.table("ctype_v4.txt", sep = "\t", header = 1, row.names = 1) df_cells$source = plyr::mapvalues(df_cells$sample_group, from = c("Engineered-Cre", "Eng...
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--- title: "DRAFT_rpechor_gex_cell_type_annotation" author: "Drew Voigt, Nate Mullin" date: "11/04/2022" output: html_document editor_options: chunk_output_type: console --- ```{r setup, include=FALSE} library("Seurat") library(stringr) library(tidyverse) ``` This object was run with the following filters: nFeatur...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') #COL_LIST=list(DType=c('scATAC'='indianred1','scRNA'='grey60'), # CType=c('B'='blue3','Mono'='gold1','T'='r...
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--- title: "Figure 10" output: html_notebook --- # Libraries ```{r message=FALSE, warning=FALSE, paged.print=FALSE} rm(list = ls()) library(dplyr) library(ggpubr) library(ggplot2) library(rstatix) library(magrittr) library(knitr) library(kableExtra) library(openxlsx) ``` # Data ```{r} df.all ...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') COL_LIST=list(DType=c('scATAC'='indianred1','scRNA'='grey60'), CType=c('B'='blue3','Mono'='gold1','T'='red...
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library(Seurat) library(SeuratWrappers) library(monocle3) library(ggplot2) library(ggridges) library(dplyr) library(ggridges) library(cowplot) # find trajectory of d101 only organoids organoids = readRDS("../../v2_filtered/combined_all_organoids_filtered_v3.rds") organoids_eng = organoids[, organoids$source == "Engin...
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postprocess_distinct_variable_selection <- function( input_obj, input_mat = NULL, logpval_vec, #larger is more significant cor_threshold = 0.9, input_assay = 2, min_subsample_cell = NULL, num_variables = 10, seurat_celltype_variable = "celltype", seurat_obj = NULL, verbose = 1 ){...
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context("Test .evaluate_radian") ## .evaluate_radian is correct test_that(".evaluate_radian works", { # load("tests/assets/test_data1.RData") load("../assets/test_data1.RData") score_1 <- test_data$score_1 score_2 <- test_data$score_2 svd_1 <- test_data$svd_1 svd_2 <- test_data$svd_2 basis_list <- test_...
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# Plot GLM (with interaction) absolute values library(tidyverse) library(patchwork) library(ggpubr) library(rstatix) # Load data load("processed_data/Results/df_GLM_absolute.Rdata") load("processed_data/Results/df_GLM_sig_absolute.Rdata") # Change factor labels levels(dfabs_GLM$modality) <- list("Auditory" = "audit...
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#' Plotting function for bi-directional FR-Match results #' #' This function combines and plots two sets of reciprocal \code{\link[FRmatch]{FRmatch}} results (i.e. experiment 1 (E1) query #' to experiment 2 (E2) reference with E2 markers, and E2 query to E1 reference with E1 markers). #' #' @param rst.FRmatch.E1toE2,r...
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#' Alternating Background Colour #' #' Add alternating background color along the y-axis. The geom takes default #' aesthetics \code{odd} and \code{even} that receive color codes. The codes #' would preferably be in the 8-hex ARGB format to allow for transparency if #' the geom is meant to be used as visual background....
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') .getYesNo<-function(DType,CType){ DType=DType CType=CType i.yes=c() j.yes=c() i.no=c() j.no=c() ...
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# CoDa analysis of fractions spent in each of 8 behaviors require(data.table) require(compositions) require(npmv) library(plyr) library(rstatix) # for visualization require(ggplot2) library(ggpubr) # load data set dat <- read.table("../Output/Mouse_behavior_data_set.csv",header = TRUE, sep = ",") dat <- within(dat,...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') .getYesNo<-function(DType,CType){ DType=DType CType=CType i.yes=c() j.yes=c() i.no=c() j.no=c() ...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') .getYesNo<-function(DType,CType){ DType=DType CType=CType i.yes=c() j.yes=c() i.no=c() j.no=c() ...
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panel <- c("heart", "hspc", "intestine_colon", "liver", "lung", "neuron", "skeletal_muscle") other <- c("esc", "endothelial_cell", "fibroblast", "kidney", "b_cell", "t_cell", "monocyte_macrophage") golden_exists <- function(name) file.exists(file.path(repo_root, "tests", "golden", paste0(name, ".rds"))) eq <- example_q...
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--- title: "SiT - Mouse Olfactory Bulb (MOB) - 02.nanopore" author: '[Kevin Lebrigand](mailto:lebrigand@ipmc.cnrs.fr)§, Joseph Bergenstråhle §, Kim Thrane §, Annelie Mollbrink, Pascal Barbry, Rainer Waldmann and Joakim Lundeberg' date: 'Compiled: `r format(Sys.Date(), "%B %d, %Y")`' abstract: 'The spatial landscape of ...
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################################################################################################### ##### Processing of WT Data ##### ################################################################################################### { library(Seurat) library(Signac) library(Ge...
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################################################################################################### ##### Processing of Mutant Data ##### ################################################################################################### { library(Seurat) library(Signac) library(...
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###To find markers for individual clusters DefaultAssay(obj) <- "RNA" obj <- NormalizeData(obj, verbose=FALSE) all_markers <- FindAllMarkers(object = obj, only.pos = TRUE, min.pct = 0.25, logfc.threshold = 0.25) print(all_m...
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# Attach the CBDA library suppressPackageStartupMessages(library(CBDA)) suppressPackageStartupMessages(library(randomForest )) suppressPackageStartupMessages(library(glmnet )) suppressPackageStartupMessages(library(SuperLearner)) suppressPackageStartupMessages(library(bartMachine)) library(CBDA, quietly = TRUE,...
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--- title: "dge_plotting_11042022" author: "Nate Mullin" date: "11/04/2022" output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) library(Signac) library(Seurat) library(patchwork) library(tidyverse) library(ggpubr) library(patchwork) library(EnhancedVolcano) library(RColorBrewer) li...
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##' A version of the checkLM function from Morpho that plots specimens by module identity #' #' @param lm.tibble a tibble of landmark data #' @return The sum of \code{x} and \code{y}. #' @examples #' color.palette <- c( "#6a3d9a","dimgrey","#fb9a99", "gold", "#009E73", "#D55E00", "#CC79A7", "cyan2", "#e31a1c", "#007...
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--- title: "Random Forest Variable Importance" author: "Andrew Soncrant" output: html_document --- ```{r setup, include=FALSE} knitr::opts_chunk$set(echo = TRUE) knitr::opts_knit$set(root.dir = "~/GitHub/CBDA/Pipeline/DISTRIBUTED/BINOMIALdataset/TEST") library(gridExtra) library(ggplot2) ``` ## Overview Here we look...
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--- title: "SiT - Mouse Coronal Brain Section (CBS1) - 02.nanopore" author: '[Kevin Lebrigand](mailto:lebrigand@ipmc.cnrs.fr)§, Joseph Bergenstråhle §, Kim Thrane §, Annelie Mollbrink, Pascal Barbry, Rainer Waldmann and Joakim Lundeberg' date: 'Compiled: `r format(Sys.Date(), "%B %d, %Y")`' abstract: 'The spatial lands...
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--- title: "SiT - Mouse Coronal Brain Section (CBS2) - 02.nanopore" author: '[Kevin Lebrigand](mailto:lebrigand@ipmc.cnrs.fr)§, Joseph Bergenstråhle §, Kim Thrane §, Annelie Mollbrink, Pascal Barbry, Rainer Waldmann and Joakim Lundeberg' date: 'Compiled: `r format(Sys.Date(), "%B %d, %Y")`' abstract: 'The spatial lands...
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--- title: "Figure 5 Stats" output: html_notebook --- ```{r message=FALSE, warning=FALSE, echo=FALSE,include=FALSE} rm(list = ls()) library(lme4) library(lmerTest) library(emmeans) library(dplyr) library(ggpubr) library(ggplot2) library(rstatix) library(effsize) library(magrittr) library(knitr) librar...
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library(Seurat) library(glmnet) library(ggplot2) library(cowplot) library(foreach) library(doMC) # getPopulationOffset = function(y){ if(!is.factor(y)) y=factor(y) if(length(levels(y))!=2) stop("y must be a two-level factor") off = sum(y==levels(y)[2])/length(y) off = log(off/(1-off)) return(rep(off,...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') .getYesNo<-function(DType,CType){ DType=DType CType=CType i.yes=c() j.yes=c() i.no=c() j.no=c() ...
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# /home/toolkit/tools/R4.2.0/bin/R library(Signac) library(GenomicRanges) library(Seurat) #args <- commandArgs(TRUE) #opath=args[1] source('https://gitee.com/jumphone/BEER/raw/master/BEER.R') #COL_LIST=list(DType=c('scATAC'='indianred1','scRNA'='grey60'), # CType=c('B'='blue3','Mono'='gold1','T'='r...
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context("forestplot") library(ggplot2) library(vdiffr) library(magrittr) # Define df linear case df_linear <- df_linear_associations %>% dplyr::arrange(name) %>% dplyr::filter(dplyr::row_number() < 30) test_that("forestplot plots linear results", { vdiffr::expect_doppelganger( title = "Associations to BM...
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######################## Description and Setup ################################# # This script performs statistical analysis of normalized cortical thickness # Load Libraries library(sciplot) library(rstatix) library(ggthemes) library(tidyverse) # load preconfigured custom plot theme source('code/analysis/paper_plot_...
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# Data preprocessing Part 2 library(tidyverse) # Load data load("processed_data/allValues/allValuesAuditory_before_DPP_pt2.Rda") load("processed_data/allValues/allValuesVestibular_before_DPP_pt2.Rda") load("processed_data/allValues/allValuesVisual_before_DPP_pt2.Rda") ### === AUDITORY === ### # Convert intensity l...
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#' @title metNor #' @description Normalize data using different normalization methods. #' @author Xiaotao Shen #' \email{shenxt@@sioc.ac.cn} #' @param ms1.data.name MS1 peak table name. Default is "data.csv". #' @param sample.info.name sample.info name. Default is "sample.info.csv" #' @param minfrac.qc Default is 0. #'...
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#' Gather bibliographic content from PubMed database using NCBI entrez APIs #' #' It gathers metadata about publications from the NCBI PubMed database. #' The use of NCBI PubMed APIs is entirely free, and doesn't necessarily require an API key. #' The function \code{pmApiRequest} queries NCBI PubMed using an entrez que...
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--- output: github_document --- <!-- README.md is generated from README.Rmd. Please edit that file --> ```{r, echo = FALSE} knitr::opts_chunk$set( collapse = TRUE, comment = "#>", fig.path = "man/figures/README-", message = FALSE ) library(magrittr) library(ggforestplot) ``` <br> <div style="text-align:lef...
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## VERY IMPORTANT -- SCRIPT 1 [FOR THE PIPELINE WORKFLOW] #!/usr/bin/env Rscript #args = commandArgs(trailingOnly=TRUE) #print(args) #arg_file = as.array(args[1]) #print(arg_file) #j_global=as.numeric(args[2]) #dataset_file = as.array(args[2]) #workspace_directory=as.array(args[4]) #workspace_directory=as.array(args[...
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# Analyze Repetition Preference # For comparison with analysis_GLM.R and analysis_GLM_LearningModels.R, include # the interaction library(tidyverse) library(DescTools) source("functions/CountRepetitionPreference.R") # Auditory | Vestibular | Visual df_auditory <- read.csv(file = "raw_data/auditoryData.csv") df_vest...
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#!/usr/bin/env Rscript # library suppressPackageStartupMessages(library("Nonpareil")) # args args = commandArgs(trailingOnly = TRUE) if(length(args) < 3){ cat('Usage: nonpareil_summary.R <seq-depth> <out-base> <npo_file1> [.npo_file...]\n') cat(' seq-depth = target sequencing depth (eg., 1e9)\n') cat(...
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--- title: "Analyse Network Simulation Data" author: "Andreas" date: "10/5/2020" params: Test: 5 output: html_document: toc: true toc_float: true theme: cerulean --- <!--******************************************************************--> <!--**********************************************************...
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## ----setup, include = FALSE--------------------------------------------------- knitr::opts_chunk$set( catch = TRUE, collapse = TRUE, comment = "#>" ) ## ----install, eval=FALSE------------------------------------------------------ # install.packages("devtools") # devtools::install_github("JCVenterInstitute/F...
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#!/usr/bin/Rscript library(caret) library(ROCR) library(xgboost) library(doMC) registerDoMC(48) args <- commandArgs(trailingOnly=TRUE) # datasets positive <- rownames(read.csv("./data/Dang.tsv", sep="\t", head=TRUE, row.names=1)) negative <- as.character(read.csv("./data/1kg_LoFT.tsv", head=FALSE)$...
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# read 'KPR all sighting' data # score # of KPR males seen in first 5min # determine 2' and 3' association surveys # summarise activity context library(tidyverse) library(here) library(janitor) library(skim) library(readxl) fn_path <- "/Volumes/WorkDrive/DAP/GoogleDriveSync/DAP_sync/Aerial/DataAnalysis/" # ids kpr_i...
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#' Make data object #' #' This function creates the data object used in \code{\link[FRmatch]{FRmatch}}. #' See an example of the data object in \code{\link[FRmatch]{sce.example}}. #' #' @param dat Cell-by-gene expression data in a data frame or equivalent format. The first column is cell names, followed by columns of ...
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library(Seurat) library(ggplot2) library(cowplot) ### visualize markers (stacked violin) ### visualize_markers_violin = function(seu_obj, cell_type_key, genes) { DefaultAssay(seu_obj) = 'RNA' Idents(seu_obj) = seu_obj@meta.data[[cell_type_key]] VlnPlot(seu_obj, features = genes, pt.size = 0, stack = T, flip = T)...