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1
However, powering EVs requires cathode materials with a higher energy density (>200 mAhg−1).
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2
The marine algal toxin domoic acid is an important threat to marine mammal health, and exposure can lead to both acute neurologic signs and a chronic epileptic syndrome in California sea lions (Zalophus californianus).
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3
Phenobarbital has been used for several decades to manage seizures, although reports are limited correlating dosing, serum monitoring and clinical efficacy in this species.
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6
Serum levels remained detectable as far as 101 days after administration, and remained stable during periods of prolonged anorexia, although dramatic decreases in serum concentrations were noted immediately after normal eating resumed.
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8
Long-term seizure control may be difficult to achieve with anti-epileptic drugs such as phenobarbital alone, and further research is needed to make novel options useful for clinical management of biotoxin-related neurologic disease in this aquatic species.
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10
As harmful algal blooms are increasing in frequency and intensity under changing ocean conditions, the resulting DA toxicosis has become increasingly common since it was first documented in this species in 1998 [2,3,4,5].
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11
Animals that exhibit these neurologic signs of DA toxicosis may strand on beaches and be rescued for rehabilitation and treatment.
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12
Depending on the clinical course of the disease, outcome options include release back into the wild, humane euthanasia, or animals may be deemed non-releasable by NOAA National Marine Fisheries Service and placed in a long-term care facility [8].
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13
For animals with chronic epilepsy, seizures are controlled with anti-epileptic drugs (AEDs).
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14
Phenobarbital (PB) has the longest history of chronic use of all AEDs in veterinary medicine and has long been the AED of choice for seizures with non-metabolic etiology in dogs and cats due to its low cost and low incidence of adverse effects [9,10,11].
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16
This report details long-term PB administration and serum PB monitoring in a single CSL with chronic epilepsy secondary to DA toxicosis.
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17
This case describes an 8-year-old castrated male CSL.
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19
A generalized convulsive seizure was observed while in rehabilitation.
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21
An initial MRI scan obtained in January 2018 showed no structural abnormalities, but a second MRI study obtained in October 2018 showed evidence of unilateral hippocampal atrophy.
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22
To control seizures, AEDs were administered throughout the study period of June 2018 to February 2022.
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25
Diazepam was administered at a dose of 0.05–0.1 mg/kg PO SID-BID, primarily for its behavior-modulating effects as opposed to its anticonvulsant activity.
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27
Lorazepam (0.1–0.2 mg/kg intramuscular (IM)) was administered when a seizure was observed.
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28
In October 2020, a xenotransplantation procedure was performed to deliver interneuron progenitor cells into the damaged hippocampus [18].
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29
Veterinary medical records were reviewed from June 2018 to February 2022.
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31
Only generalized convulsive epileptic seizures were evaluated because electroencephalographic (EEG) confirmation of non-convulsive seizure activity was unavailable.
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32
Presence of any additional neurologic signs that could be indicative of partial seizures (motor, autonomic, behavioral) were also noted.
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33
When no clinical concerns were noted, PB serum monitoring was scheduled every 4–8 weeks, primarily as a 24-h trough.
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34
Daily husbandry records were reviewed for the same time period, where any behavior changes were recorded periodically across approximately 12 h of each day.
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35
Feeding records were evaluated to assess total daily food consumption.
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36
Periods of prolonged anorexia were defined as complete inappetence with no food intake lasting longer than 48 h. During these periods, no PB was administered PO.
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38
Administration of IM PB was dependent on recent PB serum concentrations and clinical judgment.
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40
When neither PO or IM PB was administered, either during a period of prolonged anorexia or when a previous serum concentration was above a desired level, a blood sample would reflect a time point longer than 24 h after oral administration.
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42
To evaluate total daily dose recommendations and therapeutic and ideal PB serum concentrations, only trough PB serum concentrations from blood samples collected 24 h after the last oral dose were included.
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43
PB serum concentrations were measured at a reference laboratory (Idexx Laboratories, Westbrook, ME, USA).
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44
Routine complete blood count and serum biochemical analysis was performed from the same sample at the time of serum PB monitoring.
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45
To screen for evidence of potential hepatotoxicity associated with PB administration, alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT) were evaluated based on the distribution of tissue enzymes in this species [20].
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49
A complete blood count and serum biochemical analysis was paired with PB serum concentration for 49 of 63 time points.
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50
PB was administered PO for a total of 52 time points.
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51
These 35 time points were used in evaluating the PB therapeutic range for this animal.
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52
Generalized seizure control was achieved when serum PB concentrations were above 18.0 μg/mL. A total of 17 generalized convulsive seizures were observed during the study period.
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53
For this animal, 18–35 μg/mL was the observed therapeutic range, with a mean PB concentration of 23.8 μg/mL (±5.8) resulting from a mean total daily dose of 1.5 mg/kg (±0.3).
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56
Three 24-h trough samples with a mean total daily dose of 2.5 mg/kg (±1.0) resulted in supratherapeutic PB concentrations (mean 52.4 μg/mL (±16.3)).
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61
After this time point, PB was discontinued for 101 days to allow PB concentrations to fall and while alternative AEDs were investigated.
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62
PB serum levels remained above 30 μg/mL for more than 500 h (21 days), and were detectable as far as 2442 h (101 days) after administration (Figure 2).
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65
The mean pre-surgery PB concentration was 24.2 μg/mL (±15.0), compared with a mean post-surgery PB concentration of 24.0 μg/mL (±6.4) Between April and October 2020, there were four periods of time where the animal experienced prolonged anorexia, with a mean duration of 7.8 days (range 2–17 days).
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66
During this time, PB was administered as an IM injection twelve times.
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[ 55, 56, 56, 0, 7, 0, 0, 0, 0, 29, 30, 49, 50, 0 ]
67
During this six-month period, only 19% (3/16) of PB serum concentrations were between 20 and 30 μg/mL, compared with 31% (10/32) of time points throughout the rest of the study period.
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69
Unexpectedly, when normal food intake resumed, PB levels dropped dramatically at the next sample timepoint, with a mean decrease of 27% (range 16% to 47%).
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72
At the second time point, the PB level was subtherapeutic and not believed to be related to the elevated ALT concentration.
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73
No other clinically significant elevations in transaminases were observed at other time points.
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[ 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 55, 56, 56, 0 ]
74
Aiming for the reference laboratory ideal range was more appropriate for this animal, particularly given its challenges with anorexia and consistent medication administration, and how long a level above therapeutic range persisted over time.
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76
The data from this CSL, with a very slow terminal decrease in plasma concentrations, suggest the existence of a deep tissue compartment, and likely a half-life substantially longer than in humans or other veterinary species.
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77
This is supported by the fact that plasma concentrations were very similar between SID versus BID dosing, which is similar to what is reported in humans [25].
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78
Statistical tests were not performed given that this was a single animal, but differences between SID and BID dosing were unlikely to have been clinically significant.
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79
SID dosing may assist with compliance, particularly in animals with a reduced appetite.
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80
SID dosing is likely appropriate for this species, although individual variation in drug absorption may exist, and more frequent administration may be required in cases where seizures are not well-controlled.
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81
In dogs, PB can reportedly cause liver injury [26,27].
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82
No clinically significant hepatic enzyme elevations were observed in this case or have been mentioned in any of the reports of PB use in this species (Table 1), and these findings support a low incidence of hepatic enzyme induction by PB in California sea lions.
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83
This assessment was only based on biochemical changes and not gross or microscopic hepatic changes.
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84
In other species, PB can elicit gross liver enlargement, hepatocyte hypertrophy, and induced expression of drug-metabolizing enzymes [28].
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86
Administration of an AED is considered effective when a patient has a ≥50% reduction in the number of seizures during a given treatment period [23,29,30,31].
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88
Phenobarbital causes physical dependence, and rapid withdrawal of the drug itself can cause seizures [32,33].
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89
Furthermore, a 50% reduction in the number of seizures is less than desired in a marine mammal species.
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91
Near-complete eradication of generalized seizure activity is, by necessity, a more appropriate goal for aquatic species.
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92
Given restrictions in monitoring, without EEG or consistent 24 h monitoring outside of emergent clinical windows, it is unknown whether partial or subclinical seizures were present and contributing to this decline.
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94
Even during this time period, no generalized seizures were observed when PB concentrations were greater than 18 μg/mL. It is reasonable to conclude that there was good generalized seizure control with PB concentrations between 20 and 30 μg/mL when consistent administration allowed these concentrations to be reached.
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95
This animal’s quality of life was frequently assessed throughout the clinical course of treatment by multiple staff using the facility’s questionnaire.
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98
Clinicians should ideally follow standardized behavior guidelines to assess quality of life, and should be aware that certain neurologic signs, such as cluster seizures and status epilepticus, are associated with poor outcomes in epileptic patients [34].
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99
This was a retrospective study using data collected opportunistically from a complex case.
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102
This may be partially explained by a near resolution of anorexia, and thus an improvement in dosing consistency; it may also reflect gains in knowledge over time of the relationship between PB dose and concentration for this animal.
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104
The dose given for the majority of the study (0.05 mg/kg PO) is well below typical veterinary doses for seizure control (0.25–0.5 mg/kg PO) [35], and in addition, chronic PB therapy reduces peak benzodiazapine concentrations [36], making it less likely that this dose of diazepam had an impactful effect on seizure contr...
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106
Prolonged anorexia was associated with relatively small changes in PB levels during the actual period without food consumption, although the resumption of normal food intake resulted in a rapid drop in PB levels.
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107
The mechanism behind these observed changes is unknown.
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108
Phenobarbital is a lipophilic molecule.
[ "Phenobarbital", "is", "a", "lipophilic", "molecule", "." ]
[ 7, 0, 0, 7, 8, 0 ]
110
In addition, fasting differentially affects cytochrome P450-mediated drug metabolism [38], which may change how rapidly PB is cleared.
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111
Changes in pharmacokinetic parameters have been reported in dogs with differing body composition and diet [39].
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112
In marine mammals, seasonal changes such as rut associated with breeding season in intact animals or events such as clinical disease that cause dramatic changes in body weight should be closely monitored.
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113
Drug dosage should be re-evaluated if there are substantial changes in diet, body weight or body composition.
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115
Because these adverse effects overlapped with the signs associated with this animal’s neurological disease, it is difficult to conclude when side effects were an adverse effect of PB administration and when they were due to the neurological disease.
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116
The procedure led to a greater than 90% reduction in seizures and a stabilization of weight and food consumption [18].
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118
This initial research seeks to guide clinicians managing epilepsy in this species, but future pharmacokinetic analyses should be properly powered and prospectively designed.
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121
All authors had significant contributions to this study.
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122
This research received no external funding.
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125
Testing blood is essential, is done without fail as part of veterinary care, and is not an add-on for research purposes.
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128
Restrictions apply to the availability of these data.
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129
The data presented in this study are available on request from the corresponding author with the permission of Six Flags Discovery Kingdom.
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130
The authors wish to thank Kelly Goulet, Dawn Robles and Joy Middleton for their role in sample collection and processing, and to Six Flags Discovery Kingdom’s dedicated veterinary staff and training and husbandry teams for providing the highest standard of care to this sea lion, and to all of the animals in their care...
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131
In addition, the authors wish to thank the two anonymous reviewers for their feedback that strengthened the final manuscript.
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132
The authors declare no conflict of interest.
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134
SID = once daily administration.
[ "SID", "=", "once", "daily", "administration", "." ]
[ 29, 0, 29, 30, 30, 0 ]
136
PB = phenobarbital.
[ "PB", "=", "phenobarbital", "." ]
[ 7, 0, 7, 0 ]
137
d = day.
[ "d", "=", "day", "." ]
[ 55, 0, 55, 0 ]
138
a Note differences in units reported for PB concentrations.
[ "a", "Note", "differences", "in", "units", "reported", "for", "PB", "concentrations", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 7, 49, 0 ]
139
PO = per os.
[ "PO", "=", "per", "os", "." ]
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141
BID = twice daily administration.
[ "BID", "=", "twice", "daily", "administration", "." ]
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142
PB = phenobarbital.
[ "PB", "=", "phenobarbital", "." ]
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143
d = day.
[ "d", "=", "day", "." ]
[ 55, 0, 55, 0 ]
145
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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147
Based on the proposed system structure, the self-initialization scheme for state-of-charge (SOC) estimation and the fault-diagnosis scheme were tested and implemented in an actual 12-cell series battery-pack prototype.
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148
There are also broad applications in some high-power applications such as electric vehicles using large numbers of serial or parallel battery cells [3,4,5,6,7,8].
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151
It ensured the safety of the users and, in particular, prolonged the life of the battery cells in an electric vehicle with human presence.
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152
Tropical montane cloud forest may be especially sensitive to climate change.
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154
Across a 1,700–3,100 m gradient in which temperature did not covary with relative humidity (RH), in situ data showed interactions between climate and land-use.
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156
Climatologies were twice as precise for temperature as rainfall.
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158
In an era of big data, the last decade has seen a rapid increase in the number of global climatologies combining station and satellite-based data to interpolate and downscale temperature and rainfall as easily accessible gridded high-resolution datasets (Funk et al., 2015; Fick and Hijmans, 2017; Karger et al., 2017; A...
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CliReNER Silver: Named Entity Recognition for Climate Research

Dataset Summary

CliReNER silver is a fine-grained Named Entity Recognition (NER) dataset designed specifically for the climate change research domain. Recognizing the lack of broad-coverage, expert-annotated resources in Climate NLP, this dataset provides sentence-level annotations across 28 distinct entity types (27 domain-specific types + Other).

The dataset is derived from 50 full-text peer-reviewed scientific publications covering diverse topics in climate science. Sentences were pre-annotated using GLiNER and rigorously curated by the authors using a flat NER schema (nested entities are excluded).

This silver dataset consists of 1,027 sentences split into train (803), validation (106), and test (118). It is intended primarily for training and fine-tuning models. For high-fidelity, multi-expert evaluation, please refer to our companion dataset, CliReNERgold (annotated by 12 domain experts) or pre-expert-annotation version CliReNERgold author annotations.

Dataset Structure

Data Instances

A typical instance in the dataset represents a single sentence from a scientific paper, tokenized and tagged using the standard BIO (Begin, Inside, Outside) format.

{
  "id": "doc_12-sent_45",
  "text": "The increase in surface water temperature affects the local ecosystem.",
  "tokens": ["The", "increase", "in", "surface", "water", "temperature", "affects", "the", "local", "ecosystem", "."],
  "ner_tags": [0, 0, 0, 1, 2, 2, 0, 0, 0, 11, 0]
}

Data Fields

  • id: A unique string identifier for the sentence.
  • text: The original raw string of the sentence.
  • tokens: A list of strings representing the tokenized sentence.
  • ner_tags: A list of integers representing the BIO tags for each token. There are 57 tags in total (0 for O, and 1-56 representing the B- and I- tags for the 28 entity types).

Entity Typology (28 Classes)

The dataset covers a highly granular taxonomy designed for climate change literature:

  • Geographical Feature, Body of Water, Meteorological Phenomenon, Natural Phenomenon, Natural Disaster, Physical Phenomenon.
  • Organism, Ecosystem, Body Part, Disease.
  • Chemical, Energy Source, Quantity, Mathematical Expression.
  • Organization, Person, Location, Method, Measuring Device, Satellite, Physical Artefact, Intellectual Artefact, Policy, System, Asset, Field of Study, Time Period.
  • Other.

(For complete definitions of each entity type, please refer to Appendix B of the associated paper).

Dataset Creation

  • Source Data: Sentences were sampled from a corpus of 50 publications drawn from 25 peer-reviewed journals (curated by Poleksić and Martinčić-Ipšić, 2024).
  • Annotation Process: We formulated a flat NER schema to reduce cognitive load and model complexity, meaning overlapping or nested entities were resolved to the most relevant span (e.g., in "gridded rain gauge data", the full span is marked as Intellectual Artefact, subsuming the inner Measuring Device). Pre-annotation was performed via GLiNER, followed by manual author correction in Label Studio.

Limitations and Biases

  • Class Imbalance: Due to the natural distribution of scientific text, some classes (e.g., Chemical, Organism, Time Period) are highly frequent, while others (e.g., Asset, Body Part, Natural Disaster) appear sparsely.
  • Flat Schema: The dataset constrains entity overlap. Discontinuous and nested entities are not captured, which inherently results in some loss of granular information.
  • Silver Standard: This specific split (silver) was author-annotated. While highly accurate, it does not possess the multi-annotator consensus methodology applied to the gold test set.

Usage

You can easily load the dataset using the Hugging Face datasets library:

from datasets import load_dataset

# Load the dataset
dataset = load_dataset("P0L3/CliReNER_v_1_1_28_SILVER")

# View the first training example
print(dataset["train"][0])

# Access the label names (string representations of the integers)
label_names = dataset["train"].features["ner_tags"].feature.names
print(label_names)

Citation Information

@misc{poleksic2026named,
  author       = {Poleksić, Andrija and Martinčić-Ipšić, Sanda},
  title        = {Named Entity Recognition for Climate Change Research},
  year         = {2026},
  howpublished = {Research Square},
  note         = {Preprint}
}
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