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W2172196619
Calcineurin is a eukaryotic Ca 2+ - and calmodulin-dependent serine/threonine protein phosphatase. It is a heterodimeric protein consisting of a catalytic subunit calcineurin A, which contains an active site dinuclear metal center, and a tightly associated, myristoylated, Ca 2+ -binding subunit, calcineurin B. The prim...
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Upon cerebral hypoxia-ischemia (HI), apoptosis-inducing factor (AIF) can move from mitochondria to nuclei, participate in chromatinolysis, and contribute to the execution of cell death. Previous work (Cande, C., N. Vahsen, I. Kouranti, E. Schmitt, E. Daugas, C. Spahr, J. Luban, R.T. Kroemer, F. Giordanetto, C. Garrido,...
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Organic anion transporting polypeptide (OATP) 1B1 mediates the hepatic uptake of many drugs including lipid-lowering statins. Decreased OATP1B1 transport activity is often associated with increased systemic exposure of statins and statin-induced myopathy. Antimalarial drug chloroquine (CQ) is also used for long-term tr...
W2158950656
In contrast to lovastatin, pravastatin did not accumulate over the study period, which is probably one of the reasons rhabdomyolysis has been reported in lovastatin-treated but not pravastatin-treated transplant patients receiving cyclosporine immunosuppression.
W2170052837
During the last decades, transplantation has become an established tool for the treatment of terminal organ failure. Beside immunological factors, hyperlipidemia is the main problem after heart transplantation, causing rapid transplant coronary artery disease (TxCAD) and poor long-term prognosis at the beginning of the...
W2332772801
Heart-transplant recipients treated with the HMG-CoA reductase inhibitor pravastatin generally show higher plasma concentrations of this drug than control subjects. However, our data suggest that the HMG-CoA reductase inhibitor pravastatin can be used effectively in these patients receiving the immunosuppressive cyclos...
W2915342131
Data from 2016 show ongoing positive trends in short- and long-term allograft survival, and a decrease in the number of active listed candi- dates for the first time in more than a decade, with a concomitant in- crease in deceased donor kidney transplants. Transplant rates that had changed dramatically for some groups ...
W2757150230
Structure of human mTORC1 components The mTORC1 (mechanistic target of rapamycin complex 1) complex garners much attention as a signaling hub that coordinates input from growth-factor receptors and nutrient availability with metabolism and cell growth and proliferation. de Araujo et al. report the crystal structure of ...
W2483767994
Abstract Evaluation of organic anion transporting polypeptide (OATP) 1B1–mediated drug‐drug interactions (DDIs) is an integral part of drug development and is recommended by regulatory agencies. In this study we compared various prediction methods and cutoff criteria based on in vitro inhibition data to assess the pote...
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OATP1B1 and OATP1B3 mediate hepatic uptake of many drugs (e.g., statins) and can mediate transporter-mediated drug-drug-interactions (DDIs). Bortezomib is the first-in-class proteasome inhibitor drug approved by the U. S. Food and Drug Administration for the treatment of multiple myeloma. The potential of bortezomib to...
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Present studies determined the effects of pretreatment with rifampicin, an organic anion-transporting polypeptide (OATP) inhibitor, and the tyrosine kinase inhibitor dasatinib on OATP1B1- and OATP1B3-mediated transport, and evaluated the OATP-mediated drug-drug interaction potential of dasatinib using the static R-valu...
W2710342185
OATP1B3 is a 12 transmembrane domain protein expressed at the basolateral membrane of human hepatocytes where it mediates the uptake of numerous drugs and endogenous compounds. Previous western blot results suggest the formation of OATP1B3 multimers. In order to better understand the function of OATP1B3 under normal ph...
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We report novel findings of increased phosphorylation, but not impaired membrane localization, in association with the reduced transport function of the V174A-OATP1B1.
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Objective: To review the available literature that provides evidence for the absence of statin interactions with tacrolimus compared with cyclosporine. Data Sources: A literature search of PubMed was performed (1990 to June 2019) using the following search terms: calcineurin inhibitors, tacrolimus, cyclosporine, statin...
W3025138167
Abstract December 2019 saw the emergence of a new epidemic of pneumonia of varying severity, called coronavirus disease 2019 (COVID-19), caused by a newly identified coronavirus, severe acute respiratory syndrome coronavirus (SARS-CoV-2). No therapeutic option is available to treat this infection that has already kille...
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This protocol describes immunoprecipitation of proteins associated with FLAG-tagged recombinant proteins followed by mass spectrometry-based proteomics to identify the associated interactome components. FLAG epitope was chosen, because existing high-affinity monoclonal antibodies allow for sensitive immunoprecipitation...
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Abstract The aim of the UniProt Knowledgebase is to provide users with a comprehensive, high-quality and freely accessible set of protein sequences annotated with functional information. In this publication we describe enhancements made to our data processing pipeline and to our website to adapt to an ever-increasing i...
W4224235286
Membrane transport proteins are involved in the absorption, disposition, efficacy, and/or toxicity of many drugs. Numerous mechanisms (e.g., nuclear receptors, epigenetic gene regulation, microRNAs, alternative splicing, post‐translational modifications, and trafficking) regulate transport protein levels, localization,...
W3133661357
Abstract Purpose: OATP1B1 (SLCO1B1) is the most abundant and pharmacologically relevant uptake transporter in the liver and a key mediator of xenobiotic clearance. However, the regulatory mechanisms that determine OATP1B1 activity remain uncertain, and as a result, unexpected drug–drug interactions involving OATP1B1 su...
W4221136501
Abstract Cyclophilins, enzymes with peptidyl‐prolyl cis/trans isomerase activity, are relevant to a large variety of biological processes. The most abundant member of this enzyme family, cyclophilin A, is the cellular receptor of the immunosuppressive drug cyclosporine A (CsA). As a consequence of the pathophysiologica...
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The organic anion transporting polypeptide family member (OATP) 1B3 is a hepatic uptake transporter that has a broad substrate recognition and plays a significant role in regulating elimination of endogenous biomolecules or xenobiotics. OATP1B3 works in tandem with OATP1B1, with which it shares approximately 80% sequen...
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W2018818236
Synthetic hydrogels have been molecularly engineered to mimic the invasive characteristics of native provisional extracellular matrices: a combination of integrin-binding sites and substrates for matrix metalloproteinases (MMP) was required to render the networks degradable and invasive by cells via cell-secreted MMPs....
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ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCombined infrared and electron diffraction study of the polymorphism of native cellulosesJunji Sugiyama, Jan Persson, and Henri ChanzyCite this: Macromolecules 1991, 24, 9, 2461–2466Publication Date (Print):April 29, 1991Publication History Published online1 May 2002Published...
W1488563510
Three-dimensional synthetic gels that mimic the extracellular matrix provide a promising tool for studying cell interactions.
W2013298034
By covalently connecting taxol with a motif that is prone to self-assemble, we successfully generate the precursor (5a), the hydrogelator (5b), and hydrogel of a taxol derivative without compromising the cytotoxic activity of the taxol. This approach promises a general method to create nanofibers of therapeutic molecul...
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An approach based on an enzyme-responsive chemically crosslinked hydrogel that undergoes a macroscopic transition such as hydrogel swelling, when triggered by a target protease, resulting in release of entrapped molecules was analyzed. Polyethylene glycol (PEG)-based crosslinked polymers were modified with peptides pro...
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A β-galactosidase-responsive molecular hydrogelator of a nitric oxide (NO) donor can release NO in a controllable manner to improve wound healing.
W2092076884
A bacterial cellulose—chitosan (BC—Chi) composite has been successfully prepared by immersing wet BC pellicle in chitosan acetic acid solution followed by freeze-drying. By changing chitosan concentration and immersion time, a foam-like structure is made with the chitosan content in the range of 12—45 per cent. Scannin...
W2610784388
Wet, soft, squishy, and tunable Hydrogels are highly cross-linked polymer networks that are heavily swollen with water. Hydrogels have been used as dynamic, tunable, degradable materials for growing cells and tissues. Zhang and Khademhosseini review the advances in making hydrogels with improved mechanical strength and...
W2129431680
Designer Hydrogels Hydrogels, which consist of highly water swollen cross-linked polymer networks, can now be made with a range of chemistries and a combination of physical and chemical cross-links. They can also be designed to degrade gradually when exposed to chemical or biological signals and are thus finding use in...
W2101101478
Being responsive and adaptive to external stimuli is an intrinsic feature characteristic of all living organisms and soft matter. Specifically, responsive polymers can exhibit reversible or irreversible changes in chemical structures and/or physical properties in response to a specific signal input such as pH, temperat...
W2239850325
We survey advances in bioanalytical and biomedical applications of functional nucleic acid-based hydrogels in this review.
W2168199960
An easy and versatile approach to construct DNA hydrogels is reported. The DNA building blocks, a Y-scaffold and a linker, can self-assemble into DNA hydrogels by the hybridization of their "sticky ends". It is shown that the DNA hydrogels undergo a sol-gel transition upon heat or enzymatic treatment, and these respons...
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Self-healing, one of the exciting properties of materials, is frequently used to repair the damage of biological and artificial systems. Here we have used enzymatic catalysis approaches to develop a fast self-healing hydrogel, which has been constructed by dynamic aldimine cross-linking of pillar[5]arene-derivant and d...
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Abstract High temperature brings adverse impacts on the energy efficiency, and even destroys a semiconductor device. Here, a novel and cost‐effective strategy is proposed to boost the energy efficiency of semiconductor devices by using the self‐adaptive evaporative cooling of a lithium‐ and bromine‐enriched polyacrylam...
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Abstract Mechanical signals such as pressure and strain reflect important psychological and physiological states of the human body. Body‐integrated sensors, including skin‐mounted and surgically implanted ones, allow personalized health monitoring for the general population as well as patients. However, the development...
W3003156620
The rational use of intrinsic and chemically induced defects makes zeolite dissolution go beyond the limit set by the zeolite structure and synthesis conditions and allows the tuning of zeolite porosity and acidity at the meso- and nano-scale.
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Abstract To understand the underlying mechanisms of progressive neurophysiological phenomena, neural interfaces should interact bi-directionally with brain circuits over extended periods of time. However, such interfaces remain limited by the foreign body response that stems from the chemo-mechanical mismatch between t...
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Abstract 3D culture of cells in designer biomaterial matrices provides a biomimetic cellular microenvironment and can yield critical insights into cellular behaviours not available from conventional 2D cultures. Hydrogels with dynamic properties, achieved by incorporating either degradable structural components or reve...
W3217747950
Abstract Due to the moist environment and inevitable movement, efficient wound closure and healing of vulnerable joint skin remains a great challenge. Herein, a poly(γ‐glutamic acid)‐crosslinked amino‐functionalized PEGylated poly(glycerol sebacate) (γ‐PGA/PEGS‐NH 2 ) adhesive hydrogel is reported. PEGS‐NH 2 and γ‐PGA ...
W3109512135
Abstract Although various biomimetic soft materials that display structural hierarchies and stimuli responsiveness have been developed from organic materials, the creation of their counterparts consisting entirely of inorganic materials presents an attractive challenge, as the properties of such materials generally dif...
W3173480697
A multilayer hydrogel nanofilm on the cell surface via enzymatic reactions provides a new platform for β cell–based therapy.
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Hydrogels have been widespreadly used in various fields. But weak toughness has limited their further applications. In this study, Dendrobium officinale enzyme (DOE) was explored to improve chitosan/γ-poly(glutamic acid) (CS/γ-PGA) hydrogel in the structure and properties. The results indicated that DOE with various si...
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Abstract Effective reprogramming of chronic wound healing remains challenging due to the limited drug delivery efficacy hindered by physiological barriers, as well as the inappropriate dosing timing in distinct healing stages. Herein, a core-shell structured microneedle array patch with programmed functions (PF-MNs) is...
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Abstract According to databases such as OMIM, Humsavar, Clinvar and Monarch, 1494 human enzymes are presently associated to 2539 genetic diseases, 75% of which are rare (with an Orphanet code). The Mondo ontology initiative allows a standardization of the disease name into specific codes, making it possible a computati...
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Abstract In the current discourse on the transition from school to work, career decision‐making has a pivotal but paradoxical position. Sociological literature emphasises the dominance of socially‐structured pathways, whilst policy‐making operates on assumptions of individual freedom to choose. In this paper we draw on...
W2080561460
In this study, the authors surveyed undergraduate students at a large northeastern business school concerning influences on their choices of major. The authors examined factors such as why students initially select particular majors and which positive and negative factors relate to any later changes in those choices. R...
W1991072383
The aim of the study was to examine the influences and motivations, on which students base their choice of career. 348 young people aged 14-18 years, completed a questionnaire and took part in an interview concerning their choice of career. It was found the greatest influence on their choice of career was their parents...
W2091497841
This study investigates the relative influence of career-choice factors on accounting students from different cultural backgrounds. As a measure of cultural variability, the individualism- collectivism dimension tends to polarize Australian students relative to Hong Kong and Taiwanese students. For this reason, this st...
W1569795728
In the face of declining state support, many universities have introduced differential pricing by undergraduate program as an alternative to across-the-board tuition increases. This practice aligns price more closely with instructional costs and students’ ability to pay postgraduation. Exploiting the staggered adoption...
W2159315953
The Chaos Theory of Careers (CTC; Pryor & Bright, 2011) construes both individuals and the contexts in which they develop their careers in terms of complex dynamical systems. Such systems perpetually operate under influences of stability and change both internally and in relation to each other. The CTC introduces n...
W2304955216
Jeffrey T. Denning and Patrick Turley Jeffrey T. Denning is an assistant professor of economics at Brigham Young University. Patrick Turley is a postdoctoral fellow at the Analytic and Translational Genetic Unit at Massachusetts General Hospital.
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We use the American Community Survey (ACS) to investigate the extent to which college major decisions were affected during and after the Great Recession with special attention to business and science, technology, engineering, and mathematics (STEM) fields, as well as the heterogeneity across demographic groups. Several...
W2764553491
Abstract For more than 20 years, both careers education and guidance have drawn much of their rationale from the DOTS analysis which analyses practice for coverage of decision learning (D), opportunity awareness (O), transition learning (T), and self awareness (S). Its assumptions are rooted in theory, unifying the aim...
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