Entry
stringlengths
6
10
Entry Name
stringlengths
5
11
Sequence
stringlengths
2
35.2k
EC number
stringlengths
7
118
Cofactor
stringlengths
38
1.77k
Gene Ontology (biological process)
stringlengths
18
11.3k
Gene Ontology (cellular component)
stringlengths
17
1.75k
Gene Ontology (molecular function)
stringlengths
24
2.09k
Pfam
stringlengths
8
232
Gene3D
stringlengths
10
250
Protein families
stringlengths
9
237
Post-translational modification
stringlengths
16
8.52k
Subcellular location [CC]
stringlengths
29
6.18k
Catalytic activity
stringlengths
64
35.7k
Kinetics
stringlengths
69
11.7k
Pathway
stringlengths
27
908
pH dependence
stringlengths
64
955
Temperature dependence
stringlengths
70
1.16k
Function [CC]
stringlengths
17
15.3k
Organism
stringlengths
8
196
P0AE67
CHEY_ECOLI
MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM
null
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:8176739}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000269|PubMed:8176739};
aerotaxis [GO:0009454]; bacterial-type flagellum-dependent swimming motility [GO:0071977]; chemotaxis [GO:0006935]; internal peptidyl-lysine acetylation [GO:0018393]; phosphorelay signal transduction system [GO:0000160]; regulation of bacterial-type flagellum-dependent cell motility [GO:1902021]; regulation of chemotax...
bacterial-type flagellum [GO:0009288]; bacterial-type flagellum basal body, C ring [GO:0009433]; bacterial-type flagellum rotor complex [GO:0120107]; cytoplasm [GO:0005737]; cytosol [GO:0005829]
acetyltransferase activity [GO:0016407]; histidine phosphotransfer kinase activity [GO:0009927]; magnesium ion binding [GO:0000287]; phosphorelay response regulator activity [GO:0000156]; phosphorelay sensor kinase activity [GO:0000155]
PF00072;
3.40.50.2300;
null
PTM: Phosphorylated by CheA or acetylated by acetyl-CoA synthetase, depending on which acetate metabolism pathway is available. The major acetylation site seems to be Lys-92. Dephosphorylated (inactivated) by CheZ. {ECO:0000269|PubMed:11359578, ECO:0000269|PubMed:1390767, ECO:0000269|PubMed:2689446, ECO:0000269|PubMed:...
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
null
null
null
null
null
FUNCTION: Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. O...
Escherichia coli (strain K12)
P0AE70
MAZF_ECOLI
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLIKAKINVLIG
3.1.27.-
null
defense response to virus [GO:0051607]; mRNA catabolic process [GO:0006402]; negative regulation of cell growth [GO:0030308]; positive regulation of programmed cell death [GO:0043068]; quorum sensing [GO:0009372]; regulation of DNA-templated transcription [GO:0006355]; regulation of growth [GO:0040008]; regulation of t...
protein-containing complex [GO:0032991]; toxin-antitoxin complex [GO:0110001]
DNA binding [GO:0003677]; molecular function activator activity [GO:0140677]; protein homodimerization activity [GO:0042803]; protein-containing complex binding [GO:0044877]; RNA binding [GO:0003723]; RNA endonuclease activity [GO:0004521]
PF02452;
2.30.30.110;
PemK/MazF family
PTM: (Microbial infection) ADP-ribosylated by enterobacteria phage T4. {ECO:0000269|PubMed:26395283}.
null
null
null
null
null
null
FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. A sequence-specific endoribonuclease it inhibits protein synthesis by cleaving mRNA and inducing bacterial stasis. It is stable, single-strand specific with mRNA cleavage independent of the ribosome, although translation enhances cleavage for some mRNA...
Escherichia coli (strain K12)
P0AE72
MAZE_ECOLI
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW
null
null
regulation of DNA-templated transcription [GO:0006355]; regulation of growth [GO:0040008]; single-species biofilm formation [GO:0044010]
protein-containing complex [GO:0032991]; toxin-antitoxin complex [GO:0110001]
DNA binding [GO:0003677]; double-stranded DNA binding [GO:0003690]; protein homodimerization activity [GO:0042803]; protein-containing complex binding [GO:0044877]; toxin sequestering activity [GO:0097351]
PF04014;
2.10.260.10;
PemI family
PTM: Degraded by the ClpPA and Lon proteases. {ECO:0000269|PubMed:12972253, ECO:0000269|PubMed:24375411, ECO:0000269|PubMed:8650219}.
null
null
null
null
null
null
FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system. Labile antitoxin that binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity. Is considered to be an 'addiction' molecule as the cell dies in its absence. Toxicity results when the levels of MazE decrease in the cell...
Escherichia coli (strain K12)
P0AE82
CPXA_ECOLI
MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQRQGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEMQIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLLFDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEFLAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELERIETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDN...
2.7.13.3
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:9401031}; Note=Phosphotransfer to CpxR is stimulated by Mg(2+) and/or Mn(2+). {ECO:0000269|PubMed:9401031};
cell adhesion involved in biofilm formation [GO:0043708]; cellular response to cell envelope stress [GO:0036460]; response to radiation [GO:0009314]; signal transduction [GO:0007165]
plasma membrane [GO:0005886]
ATP binding [GO:0005524]; identical protein binding [GO:0042802]; phosphoprotein phosphatase activity [GO:0004721]; phosphorelay sensor kinase activity [GO:0000155]
PF16527;PF00672;PF02518;PF00512;
1.10.287.130;3.30.565.10;3.30.450.210;
null
PTM: Autophosphorylated (PubMed:24492262, PubMed:9401031). Maximal phosphorylation of the dimeric isolated cytoplasmic domain (residues 188-457) is about 70%, suggesting the protein may be hemiphosphorylated in vivo; probably occurs via a trans-autophosphorylation mechanism, i.e. one subunit phosphorylates the other (P...
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:25207645, ECO:0000269|PubMed:3058985}; Multi-pass membrane protein {ECO:0000305|PubMed:15919996, ECO:0000305|PubMed:3058985}.
CATALYTIC ACTIVITY: Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.; EC=2.7.13.3; Evidence={ECO:0000269|PubMed:9401031};
null
null
null
null
FUNCTION: Histidine kinase member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031, PubMed:9473036). Activates CpxR by phosphorylation; has autokinase, phosphotrans...
Escherichia coli (strain K12)
P0AE85
CPXP_ECOLI
MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSSSNSRSQ
null
null
null
outer membrane-bounded periplasmic space [GO:0030288]
identical protein binding [GO:0042802]; unfolded protein binding [GO:0051082]
PF07813;
1.20.120.1490;
CpxP/Spy family
PTM: Degraded by DegP; some CpxP mutant proteins are more susceptible to the protease than others (PubMed:16166523). Degradation probably occurs when CpxP is associated with some misfolded proteins; overexpression of PapE leads to DegP-mediated degradation of CpxP and PapE, which requires the N-terminus of PapE. Overex...
SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:25207645, ECO:0000269|PubMed:9473036}.
null
null
null
null
null
FUNCTION: Acts as an auxiliary protein in the Cpx two-component envelope stress response system, helping modulate the Cpx response systems response to some inducers (PubMed:16303867, PubMed:25207645). Binds the periplasmic domain of sensor histidine kinase CpxA, inhibiting induction of the Cpx envelope stress response ...
Escherichia coli (strain K12)
P0AE88
CPXR_ECOLI
MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDSIDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPFNDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGTEFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWFKTLRGRGYLMVSAS
null
null
cell adhesion [GO:0007155]; negative regulation of DNA-templated transcription [GO:0045892]; positive regulation of DNA-templated transcription [GO:0045893]; regulation of cell-substrate adhesion [GO:0010810]; regulation of DNA-templated transcription [GO:0006355]
cytosol [GO:0005829]; protein-DNA complex [GO:0032993]
DNA-binding transcription factor activity [GO:0003700]; phosphorelay response regulator activity [GO:0000156]; transcription cis-regulatory region binding [GO:0000976]
PF00072;PF00486;
3.40.50.2300;6.10.250.690;1.10.10.10;
null
PTM: Phosphorylated by CpxA. {ECO:0000269|PubMed:17259177, ECO:0000269|PubMed:9401031, ECO:0000305|PubMed:7883164}.
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
null
null
null
null
null
FUNCTION: Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Required for efficient binding of stationary phase cells to hydrophobic surfa...
Escherichia coli (strain K12)
P0AEA8
CYSG_ECOLI
MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALAFIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNVVDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQLRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEVVLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNA...
1.3.1.76; 2.1.1.107; 4.99.1.4
null
cobalamin biosynthetic process [GO:0009236]; methylation [GO:0032259]; response to osmotic stress [GO:0006970]; siroheme biosynthetic process [GO:0019354]
null
NAD binding [GO:0051287]; precorrin-2 dehydrogenase activity [GO:0043115]; protein homodimerization activity [GO:0042803]; sirohydrochlorin ferrochelatase activity [GO:0051266]; uroporphyrin-III C-methyltransferase activity [GO:0004851]
PF10414;PF13241;PF14824;PF00590;
3.40.50.720;1.10.8.210;
Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family; Precorrin methyltransferase family
null
null
CATALYTIC ACTIVITY: Reaction=2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) + precorrin-2 + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:32459, ChEBI:CHEBI:15378, ChEBI:CHEBI:57308, ChEBI:CHEBI:57856, ChEBI:CHEBI:58827, ChEBI:CHEBI:59789; EC=2.1.1.107; Evidence={ECO:0000255|HAMAP-Rule:MF_01646, ECO:0000269|PubM...
null
PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. {ECO:0000255|HAMAP-Rule:MF_01646, ECO:0000305|PubMed:2407234}.; PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. {ECO:0000255|HAMAP-Rule:MF_01646, ...
null
null
FUNCTION: Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlo...
Escherichia coli (strain K12)
P0AEB2
DACA_ECOLI
MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLG...
3.4.16.4; 3.5.2.6
null
cell wall organization [GO:0071555]; peptidoglycan biosynthetic process [GO:0009252]; peptidoglycan metabolic process [GO:0000270]; proteolysis [GO:0006508]; regulation of cell shape [GO:0008360]
outer membrane-bounded periplasmic space [GO:0030288]; plasma membrane [GO:0005886]
beta-lactamase activity [GO:0008800]; carboxypeptidase activity [GO:0004180]; penicillin binding [GO:0008658]; protein homodimerization activity [GO:0042803]; serine-type D-Ala-D-Ala carboxypeptidase activity [GO:0009002]
PF07943;PF00768;
2.60.410.10;3.40.710.10;
Peptidase S11 family
null
SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Note=N-terminal lies in the periplasmic space.
CATALYTIC ACTIVITY: Reaction=Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.; EC=3.4.16.4; CATALYTIC ACTIVITY: Reaction=a beta-lactam + H2O = a substituted beta-amino acid; Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI...
null
PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
null
null
FUNCTION: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.
Escherichia coli (strain K12)
P0AEC3
ARCB_ECOLI
MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTMVLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDLSLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSFLDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEKVFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDALERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKY...
2.7.13.3
null
protein autophosphorylation [GO:0046777]; regulation of DNA-templated transcription [GO:0006355]; response to oxygen levels [GO:0070482]; signal transduction [GO:0007165]
cytosol [GO:0005829]; plasma membrane [GO:0005886]
ATP binding [GO:0005524]; phosphoprotein phosphatase activity [GO:0004721]; phosphorelay sensor kinase activity [GO:0000155]
PF02518;PF00512;PF18415;PF01627;PF00989;PF00072;
1.10.287.130;3.40.50.2300;1.10.287.970;3.30.565.10;1.20.120.160;3.30.450.20;
null
PTM: Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000269|PubMed:15919996}.
CATALYTIC ACTIVITY: Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.; EC=2.7.13.3;
null
null
null
null
FUNCTION: Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.
Escherichia coli (strain K12)
P0AEC5
BARA_ECOLI
MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDAGASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFHLDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYIALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRRGQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQNVELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLK...
2.7.13.3
null
cellular hyperosmotic response [GO:0071474]; response to acetate [GO:0010034]; response to formic acid [GO:1901425]; response to hydrogen peroxide [GO:0042542]
plasma membrane [GO:0005886]
ATP binding [GO:0005524]; histidine phosphotransfer kinase activity [GO:0009927]; phosphoprotein phosphatase activity [GO:0004721]; phosphorelay sensor kinase activity [GO:0000155]; protein histidine kinase activity [GO:0004673]; protein homodimerization activity [GO:0042803]
PF00672;PF02518;PF00512;PF01627;PF00072;PF09984;
1.10.287.130;3.40.50.2300;6.10.340.10;3.30.565.10;1.20.120.160;
null
PTM: Autophosphorylated (PubMed:11022030, PubMed:1574005). Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain (Probable). {ECO:0000269|PubMed:11022030, ECO:0000269|PubMed:1574005, ...
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000255}.
CATALYTIC ACTIVITY: Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.; EC=2.7.13.3; Evidence={ECO:0000305|PubMed:11022030};
null
null
null
null
FUNCTION: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system (PubMed:12193630, PubMed:12533459). Phosphorylates UvrY, probably via a four-step phosphorelay (PubMed:11022030). {ECO:0000269|PubMed:11022030, ECO:0000269|PubMed:1219363...
Escherichia coli (strain K12)
P0AEC8
DCUS_ECOLI
MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQIGVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAIST...
2.7.13.3
null
phosphorelay signal transduction system [GO:0000160]; regulation of DNA-templated transcription [GO:0006355]; signal transduction [GO:0007165]
cytosol [GO:0005829]; outer membrane-bounded periplasmic space [GO:0030288]; plasma membrane [GO:0005886]; protein histidine kinase complex [GO:0009365]
ATP binding [GO:0005524]; identical protein binding [GO:0042802]; phosphorelay sensor kinase activity [GO:0000155]; protein histidine kinase activity [GO:0004673]
PF02518;PF00989;PF17203;PF14689;
1.10.287.130;3.30.565.10;3.30.450.20;
null
PTM: Autophosphorylated (PubMed:12167640). The phosphoryl group is rapidly transferred to DcuR (PubMed:12167640). {ECO:0000269|PubMed:12167640}.
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:12167640, ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000255}.
CATALYTIC ACTIVITY: Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.; EC=2.7.13.3; Evidence={ECO:0000305|PubMed:12167640};
null
null
null
null
FUNCTION: Member of the two-component regulatory system DcuR/DcuS (PubMed:12167640, PubMed:9765574, PubMed:9973351). Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; sdhCDAB; etc.) (PubMed:9765574, PubMed:9973351). Weakly regul...
Escherichia coli (strain K12)
P0AED7
DAPE_ECOLI
MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMDFADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLVGEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLT...
3.5.1.18
COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:3276674}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:3276674}; Note=Binds 2 Zn(2+) or Co(2+) ions per subunit. {ECO:0000269|PubMed:3276674};
diaminopimelate biosynthetic process [GO:0019877]; lysine biosynthetic process via diaminopimelate [GO:0009089]
cell division site [GO:0032153]; cytosol [GO:0005829]
cobalt ion binding [GO:0050897]; identical protein binding [GO:0042802]; succinyl-diaminopimelate desuccinylase activity [GO:0009014]; zinc ion binding [GO:0008270]
PF07687;PF01546;
3.40.630.10;
Peptidase M20A family, DapE subfamily
null
null
CATALYTIC ACTIVITY: Reaction=H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate; Xref=Rhea:RHEA:22608, ChEBI:CHEBI:15377, ChEBI:CHEBI:30031, ChEBI:CHEBI:57609, ChEBI:CHEBI:58087; EC=3.5.1.18; Evidence={ECO:0000269|PubMed:3276674};
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.41 mM for L,L-SDAP (in the presence of Zn(2+) at pH 7.0) {ECO:0000269|PubMed:3276674};
PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.
null
null
FUNCTION: Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls. {ECO:0000269|PubMed:3276674}.
Escherichia coli (strain K12)
P0AEE3
DEGS_ECOLI
MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETPASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDADQIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGNPYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTLSFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGIDQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMD...
3.4.21.107
null
cellular response to misfolded protein [GO:0071218]; protein quality control for misfolded or incompletely synthesized proteins [GO:0006515]; proteolysis [GO:0006508]
outer membrane-bounded periplasmic space [GO:0030288]; plasma membrane [GO:0005886]
identical protein binding [GO:0042802]; peptidase activity [GO:0008233]; serine-type endopeptidase activity [GO:0004252]; serine-type peptidase activity [GO:0008236]
PF13180;PF13365;
2.30.42.10;2.40.10.10;
Peptidase S1C family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305|PubMed:11442831}; Single-pass membrane protein {ECO:0000305|PubMed:11442831}. Note=It is unclear how this protein is anchored to the inner membrane, programs predict a signal sequence, but replacing the N-terminal 26 residues with a known signal sequence gives a pr...
CATALYTIC ACTIVITY: Reaction=Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.; EC=3.4.21.107;
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=33 uM for RseA-YQF {ECO:0000269|PubMed:17981123, ECO:0000269|PubMed:19836340}; Vmax=0.6 umol/sec/mg enzyme {ECO:0000269|PubMed:17981123, ECO:0000269|PubMed:19836340};
null
null
null
FUNCTION: A site-1 protease (S1P) that cleaves the peptide bond between 'Val-148' and 'Ser-149' in RseA. Part of a regulated intramembrane proteolysis (RIP) cascade. When heat shock or other environmental stresses disrupt protein folding in the periplasm, DegS senses the accumulation of unassembled outer membrane porin...
Escherichia coli (strain K12)
P0AEE5
MGLB_ECOLI
MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVRKAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNKDGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAWLSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGTVLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVG...
null
null
chemotaxis [GO:0006935]; galactose transmembrane transport [GO:0015757]; methylgalactoside transport [GO:0015765]
ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing [GO:0055052]; membrane [GO:0016020]; outer membrane-bounded periplasmic space [GO:0030288]
calcium ion binding [GO:0005509]; carbohydrate binding [GO:0030246]
PF13407;
3.40.50.2300;
Bacterial solute-binding protein 2 family
null
SUBCELLULAR LOCATION: Periplasm.
null
null
null
null
null
FUNCTION: Part of the ABC transporter complex MglABC involved in galactose/methyl galactoside import (Probable). In addition, binds D-galactose and D-glucose and plays a role in the chemotaxis towards these two sugars by interacting with the Trg chemoreceptor (PubMed:3057628, PubMed:4927373). Chemotaxis requires MglB, ...
Escherichia coli (strain K12)
P0AEE8
DMA_ECOLI
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK
2.1.1.72
null
bacterial-type DNA replication initiation [GO:1902328]; DNA-templated DNA replication [GO:0006261]; macromolecule modification [GO:0043412]; methylation [GO:0032259]; mismatch repair [GO:0006298]; response to UV [GO:0009411]
null
S-adenosyl-L-methionine binding [GO:1904047]; sequence-specific DNA binding [GO:0043565]; site-specific DNA-methyltransferase (adenine-specific) activity [GO:0009007]
PF02086;
1.10.1020.10;3.40.50.150;
N(4)/N(6)-methyltransferase family
null
null
CATALYTIC ACTIVITY: Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90615, ChEBI:CHEBI:90616;...
null
null
null
null
FUNCTION: An alpha subtype methylase that recognizes the double-stranded sequence 5'-GATC-3' and methylates A-2 on both strands (Probable) (PubMed:12654995). Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in methyl-directed mismatch repair...
Escherichia coli (strain K12)
P0AEG4
DSBA_ECOLI
MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEFFSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALGVEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKAAADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK
null
null
cellular response to antibiotic [GO:0071236]
outer membrane-bounded periplasmic space [GO:0030288]
protein disulfide isomerase activity [GO:0003756]; protein-disulfide reductase activity [GO:0015035]
PF01323;
3.40.30.10;
Thioredoxin family, DsbA subfamily
null
SUBCELLULAR LOCATION: Periplasm.
null
null
null
null
null
FUNCTION: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the...
Escherichia coli (strain K12)
P0AEG6
DSBC_ECOLI
MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPVAGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYKAPQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIWCAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQPPKEMKEFLDEHQKMTSGK
null
null
chaperone-mediated protein folding [GO:0061077]; response to copper ion [GO:0046688]
outer membrane-bounded periplasmic space [GO:0030288]; periplasmic space [GO:0042597]
protein disulfide isomerase activity [GO:0003756]; protein homodimerization activity [GO:0042803]; protein-disulfide reductase activity [GO:0015035]
PF10411;PF13098;
3.10.450.70;3.40.30.10;
Thioredoxin family, DsbC subfamily
null
SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:8168498}.
null
null
null
null
null
FUNCTION: Acts as a disulfide isomerase, interacting with incorrectly folded proteins to correct non-native disulfide bonds. DsbG and DsbC are part of a periplasmic reducing system that controls the level of cysteine sulfenylation, and provides reducing equivalents to rescue oxidatively damaged secreted proteins. Acts ...
Escherichia coli (strain K12)
P0AEH1
RSEP_ECOLI
MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETPDWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQ...
3.4.24.-
COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
cellular response to cell envelope stress [GO:0036460]; positive regulation of DNA-templated transcription [GO:0045893]; proteolysis [GO:0006508]
plasma membrane [GO:0005886]
anti-sigma factor antagonist activity [GO:0043856]; metal ion binding [GO:0046872]; metalloendopeptidase activity [GO:0004222]
PF17820;PF02163;
2.30.42.10;
Peptidase M50B family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:11689431, ECO:0000269|PubMed:11750129, ECO:0000269|PubMed:11867724, ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000269|PubMed:11689431, ECO:0000269|PubMed:11750129, ECO:0000269|PubMed:11867724, ECO:0000269|PubMed:15919996}.
null
null
null
null
null
FUNCTION: A site-2 regulated intramembrane protease (S2P) that cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of RseA. Part of a regulated intramembrane proteolysis (RIP) cascade. Acts on DegS-cleaved RseA to release the cytoplasmic domain of RseA, residue 'Val-148' of RseA may be ...
Escherichia coli (strain K12)
P0AEI1
MIAB_ECOLI
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEK...
2.8.4.3
COFACTOR: Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305|PubMed:11882645}; Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000305|PubMed:11882645};
tRNA methylation [GO:0030488]; tRNA methylthiolation [GO:0035600]
cytosol [GO:0005829]
4 iron, 4 sulfur cluster binding [GO:0051539]; metal ion binding [GO:0046872]; N6-isopentenyladenosine methylthiotransferase activity [GO:0035597]
PF04055;PF01938;PF00919;
3.40.50.12160;3.80.30.20;
Methylthiotransferase family, MiaB subfamily
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01864}.
CATALYTIC ACTIVITY: Reaction=[sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:37067, Rhea:RHEA-COMP:1...
null
null
null
null
FUNCTION: Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine. {ECO:0000255|HAMAP-Rule:MF_01864, ECO:0000269|PubMed:10572129}.
Escherichia coli (strain K12)
P0AEJ2
ENTC_ECOLI
MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAVNGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEKQASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDAAIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARRQPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWHLATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELE...
5.4.4.2
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:20079748}; Note=Binds 2 Mg(2+) ions per subunit. {ECO:0000269|PubMed:20079748};
enterobactin biosynthetic process [GO:0009239]; salicylic acid biosynthetic process [GO:0009697]
null
isochorismate synthase activity [GO:0008909]; magnesium ion binding [GO:0000287]
PF00425;
3.60.120.10;
Isochorismate synthase family
null
null
CATALYTIC ACTIVITY: Reaction=chorismate = isochorismate; Xref=Rhea:RHEA:18985, ChEBI:CHEBI:29748, ChEBI:CHEBI:29780; EC=5.4.4.2; Evidence={ECO:0000269|PubMed:17243787, ECO:0000269|PubMed:20079748, ECO:0000269|PubMed:2139795};
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=5 uM for isochorismate (at pH 7.8 and 37 degrees Celsius) {ECO:0000269|PubMed:2139795}; KM=7 uM for chorismate {ECO:0000269|PubMed:20079748}; KM=14 uM for chorismate (at pH 7.8 and 37 degrees Celsius) {ECO:0000269|PubMed:2139795}; KM=41 uM for chorismate {ECO:00002...
PATHWAY: Siderophore biosynthesis; enterobactin biosynthesis. {ECO:0000305}.
null
null
FUNCTION: Involved in the biosynthesis of the siderophore enterobactin (macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine). Catalyzes the reversible conversion of chorismate to isochorismate. {ECO:0000269|PubMed:17243787, ECO:0000269|PubMed:20079748, ECO:0000269|PubMed:2139795, ECO:0000269|PubMed:2536681,...
Escherichia coli (strain K12)
P0AEJ4
ENVZ_ECOLI
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIE...
2.7.13.3
null
phosphorelay signal transduction system [GO:0000160]; phosphorylation [GO:0016310]; protein autophosphorylation [GO:0046777]; response to osmotic stress [GO:0006970]; signal transduction [GO:0007165]
cytosol [GO:0005829]; outer membrane-bounded periplasmic space [GO:0030288]; plasma membrane [GO:0005886]
ATP binding [GO:0005524]; identical protein binding [GO:0042802]; phosphoprotein phosphatase activity [GO:0004721]; phosphorelay sensor kinase activity [GO:0000155]; protein homodimerization activity [GO:0042803]
PF00672;PF02518;PF00512;
1.10.287.130;3.30.565.10;
null
PTM: Autophosphorylated (PubMed:2277041, PubMed:2656684, PubMed:2668281, PubMed:2668953, PubMed:3056929, PubMed:8132603). Incubation of isolated EnvZ C-terminal fragment (residues 180-450) with increasing levels of NaCl or sucrose increases its autophosphorylation (PubMed:17635923). {ECO:0000269|PubMed:17635923, ECO:00...
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:1323560, ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:2824492}; Multi-pass membrane protein {ECO:0000305|PubMed:1323560, ECO:0000305|PubMed:15919996, ECO:0000305|PubMed:2824492}.
CATALYTIC ACTIVITY: Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.; EC=2.7.13.3; Evidence={ECO:0000269|PubMed:2668953, ECO:0000305|PubMed:2656684, ECO:0000305|PubMed:2668281, ECO:0000305|PubMed:2674113};
null
null
null
null
FUNCTION: Member of the two-component regulatory system EnvZ/OmpR involved in osmoregulation (particularly of genes ompF and ompC) as well as other genes (PubMed:2997120, PubMed:3536870). EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals; at low osmolar...
Escherichia coli (strain K12)
P0AEJ6
EUTB_ECOLI
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDP...
4.3.1.7
COFACTOR: Name=adenosylcob(III)alamin; Xref=ChEBI:CHEBI:18408; Evidence={ECO:0000255|HAMAP-Rule:MF_00861, ECO:0000269|PubMed:19762342, ECO:0000269|PubMed:20519496, ECO:0000269|PubMed:21142024, ECO:0000269|PubMed:29797764}; Note=Binds 1 AdoCbl between the large and small subunits, with 6 cofactors per holoenzyme. {ECO:0...
amino acid metabolic process [GO:0006520]; ethanolamine catabolic process [GO:0046336]
cytosol [GO:0005829]; ethanolamine ammonia-lyase complex [GO:0009350]; ethanolamine degradation polyhedral organelle [GO:0031471]
cobalamin binding [GO:0031419]; ethanolamine ammonia-lyase activity [GO:0008851]
PF06751;
3.20.20.70;2.30.170.30;1.10.220.70;
EutB family
null
SUBCELLULAR LOCATION: Bacterial microcompartment {ECO:0000255|HAMAP-Rule:MF_00861}.
CATALYTIC ACTIVITY: Reaction=ethanolamine = acetaldehyde + NH4(+); Xref=Rhea:RHEA:15313, ChEBI:CHEBI:15343, ChEBI:CHEBI:28938, ChEBI:CHEBI:57603; EC=4.3.1.7; Evidence={ECO:0000255|HAMAP-Rule:MF_00861, ECO:0000269|PubMed:15466038, ECO:0000269|PubMed:19762342};
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=8.2 uM for ethanolamine {ECO:0000269|PubMed:19762342}; KM=0.055 uM for adenosylcob(III)alamin {ECO:0000269|PubMed:19762342};
PATHWAY: Amine and polyamine degradation; ethanolamine degradation. {ECO:0000255|HAMAP-Rule:MF_00861}.
null
null
FUNCTION: Catalyzes the deamination of various vicinal amino-alcohols to oxo compounds (PubMed:19762342). Allows this organism to utilize ethanolamine as the sole source of nitrogen and carbon in the presence of external vitamin B12. It is spontaneously inactivated by its substrate and reactivated by EutA (PubMed:15466...
Escherichia coli (strain K12)
P0AEK2
FABG_ECOLI
MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQAISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEEWNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIGFSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANAVAFLASDEAAYITGETLHVNGGMYMV
1.1.1.100
null
biotin biosynthetic process [GO:0009102]; fatty acid biosynthetic process [GO:0006633]; fatty acid elongation [GO:0030497]; lipid biosynthetic process [GO:0008610]
cytosol [GO:0005829]
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity [GO:0004316]; identical protein binding [GO:0042802]; metal ion binding [GO:0046872]; NAD binding [GO:0051287]; NADP binding [GO:0050661]; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor [GO:0016616]
PF13561;
3.40.50.720;
Short-chain dehydrogenases/reductases (SDR) family
null
null
CATALYTIC ACTIVITY: Reaction=a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) + NADPH; Xref=Rhea:RHEA:17397, Rhea:RHEA-COMP:9916, Rhea:RHEA-COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78776, ChEBI:CHEBI:78827; EC=1.1.1.100; Evidence={ECO:0000269|PubMed:10629181, ECO:0000...
null
PATHWAY: Lipid metabolism; fatty acid biosynthesis. {ECO:0000269|PubMed:14996818, ECO:0000269|PubMed:7592873}.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is between 6.0 and 7.0. {ECO:0000269|PubMed:4381013};
null
FUNCTION: Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis. {ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:14996818, ECO:0000269|PubMed:7592873, ECO:0000269|PubMed:8631920, ECO:0000269|...
Escherichia coli (strain K12)
P0AEK4
FABI_ECOLI
MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKLKGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDGDYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMGLAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRRTVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK
1.3.1.9
null
biotin biosynthetic process [GO:0009102]; fatty acid elongation [GO:0030497]; lipid biosynthetic process [GO:0008610]; protein homotetramerization [GO:0051289]; response to antibiotic [GO:0046677]
catalytic complex [GO:1902494]; cytosol [GO:0005829]; membrane [GO:0016020]; protein-containing complex [GO:0032991]
enoyl-[acyl-carrier-protein] reductase (NADH) activity [GO:0004318]; enoyl-[acyl-carrier-protein] reductase activity [GO:0016631]; identical protein binding [GO:0042802]; NADH binding [GO:0070404]
PF13561;
3.40.50.720;
Short-chain dehydrogenases/reductases (SDR) family, FabI subfamily
null
null
CATALYTIC ACTIVITY: Reaction=a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + H(+) + NADH; Xref=Rhea:RHEA:10240, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:78784, ChEBI:CHEBI:78785; EC=1.3.1.9; Evidence={ECO:0000269|PubMed:10629181, ECO:00002...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.3 uM for trans-2-dodecenoyl-ACP (DD-ACP)(at 25 degrees Celsius and pH 8) {ECO:0000269|PubMed:17012233}; KM=22 uM for crotonyl-ACP (at 30 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:8119879}; KM=24 uM for trans-2-dodecenoyl-CoA (DD-CoA)(at 25 degrees Celsius a...
PATHWAY: Lipid metabolism; fatty acid biosynthesis. {ECO:0000269|PubMed:7592873}.; PATHWAY: Cofactor biosynthesis; biotin biosynthesis. {ECO:0000305|PubMed:20693992}.
null
null
FUNCTION: Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis. {ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:20693992,...
Escherichia coli (strain K12)
P0AEK7
FDNI_ECOLI
MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLTQTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHKVADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGIILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI
null
COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000269|PubMed:11884747}; Note=Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme. {ECO:0000269|PubMed:11884747}...
anaerobic electron transport chain [GO:0019645]; anaerobic respiration [GO:0009061]; formate oxidation [GO:0015944]; heme oxidation [GO:0006788]
formate dehydrogenase complex [GO:0009326]; membrane [GO:0016020]; plasma membrane [GO:0005886]
electron transfer activity [GO:0009055]; formate dehydrogenase (NAD+) activity [GO:0008863]; formate dehydrogenase (quinone) activity [GO:0036397]; heme binding [GO:0020037]; metal ion binding [GO:0046872]
PF01292;
1.20.950.20;
Formate dehydrogenase gamma subunit family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:11884747}; Multi-pass membrane protein {ECO:0000269|PubMed:11884747}.
null
null
null
null
null
FUNCTION: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556 component of the formate dehydrogenase-N, and also contains a menaquinone reduction site that receives electrons from the bet...
Escherichia coli (strain K12)
P0AEL0
FDOI_ECOLI
MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVALIVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE
null
COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250}; Note=Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme. {ECO:0000250};
anaerobic electron transport chain [GO:0019645]; anaerobic respiration [GO:0009061]; cellular respiration [GO:0045333]; DNA damage response [GO:0006974]; formate oxidation [GO:0015944]; heme oxidation [GO:0006788]
formate dehydrogenase complex [GO:0009326]; membrane [GO:0016020]; plasma membrane [GO:0005886]
electron transfer activity [GO:0009055]; formate dehydrogenase (NAD+) activity [GO:0008863]; formate dehydrogenase (quinone) activity [GO:0036397]; metal ion binding [GO:0046872]
PF01292;
1.20.950.20;
Formate dehydrogenase gamma subunit family
null
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
null
null
null
null
null
FUNCTION: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase.
Escherichia coli (strain K12)
P0AEM0
FKBX_ECOLI
MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASLSEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFTAMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA
5.2.1.8
null
chaperone-mediated protein folding [GO:0061077]
cytosol [GO:0005829]
peptidyl-prolyl cis-trans isomerase activity [GO:0003755]
PF00254;
2.40.10.330;3.10.50.40;
FKBP-type PPIase family
null
null
CATALYTIC ACTIVITY: Reaction=[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0); Xref=Rhea:RHEA:16237, Rhea:RHEA-COMP:10747, Rhea:RHEA-COMP:10748, ChEBI:CHEBI:83833, ChEBI:CHEBI:83834; EC=5.2.1.8; Evidence={ECO:0000269|PubMed:9188461};
null
null
null
null
FUNCTION: PPIases accelerate the folding of proteins (Probable). Substrate specificity investigated with 'Suc-Ala-Xaa-Pro-Phe-4-nitroanilide' where Xaa is the amino acid tested, was found to be Phe > Leu >> Ile > Lys = Ala > Trp > His >> Gln (PubMed:9188461). {ECO:0000269|PubMed:9188461, ECO:0000305}.
Escherichia coli (strain K12)
P0AEM6
FLIA_ECOLI
MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDLLQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQAIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELVTDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTKLGKL
null
null
bacterial-type flagellum assembly [GO:0044780]; bacterial-type flagellum-dependent cell motility [GO:0071973]; DNA-templated transcription initiation [GO:0006352]; regulation of DNA-templated transcription [GO:0006355]; regulation of DNA-templated transcription initiation [GO:2000142]
cytosol [GO:0005829]; cytosolic DNA-directed RNA polymerase complex [GO:0000345]
DNA binding [GO:0003677]; DNA-directed 5'-3' RNA polymerase activity [GO:0003899]; sigma factor activity [GO:0016987]
PF04542;PF04539;PF04545;
1.10.1740.10;1.20.140.160;
Sigma-70 factor family, FliA subfamily
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00962}.
null
null
null
null
null
FUNCTION: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor controls the expression of flagella-related genes. {ECO:0000255|HAMAP-Rule:MF_00962, ECO:0000269|PubMed:2644646, ECO:0000269|PubMed:3536871, ECO:0000269|PubM...
Escherichia coli (strain K12)
P0AEM9
TCYJ_ECOLI
MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLLVGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRIDVVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNYEEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEAFSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK
null
null
cysteine transmembrane transport [GO:1903712]; L-cystine transport [GO:0015811]; sulfur utilization [GO:0006791]
ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing [GO:0055052]; outer membrane-bounded periplasmic space [GO:0030288]
amino acid binding [GO:0016597]; L-cystine transmembrane transporter activity [GO:0015184]; ligand-gated monoatomic ion channel activity [GO:0015276]
PF00497;
3.40.190.10;
Bacterial solute-binding protein 3 family
null
SUBCELLULAR LOCATION: Periplasm {ECO:0000305|PubMed:20351115}.
null
null
null
null
null
FUNCTION: Part of the ABC transporter complex TcyJLN involved in L-cystine import (PubMed:20351115, PubMed:25139244, PubMed:25837721, PubMed:26350134). This high affinity cystine transporter is involved in resistance to oxidative stress by forming a L-cysteine/L-cystine shuttle system with the EamA transporter, which e...
Escherichia coli (strain K12)
P0AEN1
FRE_ECOLI
MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDERDKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDEERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHPGLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSERNAREDRLFGDAFAFI
1.5.1.41
null
iron ion transport [GO:0006826]; protein repair [GO:0030091]; response to oxidative stress [GO:0006979]
cytosol [GO:0005829]
enzyme activator activity [GO:0008047]; riboflavin reductase (NAD(P)H) activity [GO:0052875]; riboflavin reductase (NADPH) activity [GO:0042602]
PF00970;PF00175;
3.40.50.80;2.40.30.10;
Fre/LuxG FAD/NAD(P) flavoprotein oxidoreductase family
null
null
CATALYTIC ACTIVITY: Reaction=NADP(+) + reduced riboflavin = 2 H(+) + NADPH + riboflavin; Xref=Rhea:RHEA:19377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17607, ChEBI:CHEBI:57783, ChEBI:CHEBI:57986, ChEBI:CHEBI:58349; EC=1.5.1.41; Evidence={ECO:0000250|UniProtKB:Q9L6L9}; CATALYTIC ACTIVITY: Reaction=NAD(+) + reduced riboflavin = 2...
null
null
null
null
FUNCTION: Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. {ECO:0000250|UniProtKB:Q9L6L9}.
Escherichia coli (strain K12)
P0AEN4
FTSL_ECOLI
MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFICIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIATEKLQMQHVDPSQENIVVQK
null
null
cell division [GO:0051301]; division septum assembly [GO:0000917]; FtsZ-dependent cytokinesis [GO:0043093]
cell division site [GO:0032153]; divisome complex [GO:1990586]; FtsBL complex [GO:1990588]; FtsQBL complex [GO:1990587]; plasma membrane [GO:0005886]
null
PF04999;
null
FtsL family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-Rule:MF_00910, ECO:0000269|PubMed:10027987, ECO:0000269|PubMed:11703663, ECO:0000269|PubMed:11948172, ECO:0000269|PubMed:1332942, ECO:0000269|PubMed:15165235}; Single-pass type II membrane protein {ECO:0000255|HAMAP-Rule:MF_00910, ECO:0000269|PubMed:10027987,...
null
null
null
null
null
FUNCTION: Essential cell division protein. May link together the upstream cell division proteins, which are predominantly cytoplasmic, with the downstream cell division proteins, which are predominantly periplasmic. Can also mediate Zn(2+)-sensitivity probably by increasing the permeability of the inner membrane. {ECO:...
Escherichia coli (strain K12)
P0AEN8
FUCM_ECOLI
MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIRADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDIIRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP
5.1.3.29; 5.4.99.62
null
fucose metabolic process [GO:0006004]; fucosylation [GO:0036065]; L-fucose metabolic process [GO:0042354]
cytoplasm [GO:0005737]
D-ribose pyranase activity [GO:0062193]; fucose binding [GO:0042806]; identical protein binding [GO:0042802]; L-fucose mutarotase activity [GO:0036373]; racemase and epimerase activity, acting on carbohydrates and derivatives [GO:0016857]
PF05025;
3.40.1650.10;
RbsD / FucU family, FucU mutarotase subfamily
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CATALYTIC ACTIVITY: Reaction=alpha-L-fucose = beta-L-fucose; Xref=Rhea:RHEA:25580, ChEBI:CHEBI:42548, ChEBI:CHEBI:42589; EC=5.1.3.29; Evidence={ECO:0000269|PubMed:15060078, ECO:0000269|PubMed:19524593}; CATALYTIC ACTIVITY: Reaction=beta-D-ribopyranose = beta-D-ribofuranose; Xref=Rhea:RHEA:25432, ChEBI:CHEBI:27476, ChEB...
null
PATHWAY: Carbohydrate metabolism; L-fucose metabolism. {ECO:0000269|PubMed:15060078, ECO:0000269|PubMed:19524593}.
null
null
FUNCTION: Involved in the anomeric conversion of L-fucose. Catalyzes also the interconversion of beta-pyran and beta-furan forms of D-ribose. {ECO:0000269|PubMed:15060078, ECO:0000269|PubMed:19524593}.
Escherichia coli (strain K12)
P0AEP3
GALU_ECOLI
MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQYVVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHVTIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFDETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRYVLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAFVEYGIRHNTLGTEFKAWLEEEMGIKK
2.7.7.9
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:7961613};
colanic acid biosynthetic process [GO:0009242]; galactose catabolic process via UDP-galactose [GO:0033499]; lipopolysaccharide core region biosynthetic process [GO:0009244]; osmoregulated periplasmic glucan biosynthetic process [GO:1900727]; UDP-glucose metabolic process [GO:0006011]
cytosol [GO:0005829]; protein-containing complex [GO:0032991]
identical protein binding [GO:0042802]; magnesium ion binding [GO:0000287]; UTP:glucose-1-phosphate uridylyltransferase activity [GO:0003983]
PF00483;
null
UDPGP type 2 family
null
null
CATALYTIC ACTIVITY: Reaction=alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose; Xref=Rhea:RHEA:19889, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:58601, ChEBI:CHEBI:58885; EC=2.7.7.9; Evidence={ECO:0000269|PubMed:7961613};
null
null
null
null
FUNCTION: May play a role in stationary phase survival.
Escherichia coli (strain K12)
P0AEP7
GCL_ECOLI
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVS...
4.1.1.47
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250}; COFACTOR: Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000250}; Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000250};
glycolate catabolic process [GO:0046296]; glyoxylate catabolic process [GO:0009436]; isoleucine biosynthetic process [GO:0009097]; valine biosynthetic process [GO:0009099]
acetolactate synthase complex [GO:0005948]
FAD binding [GO:0071949]; flavin adenine dinucleotide binding [GO:0050660]; identical protein binding [GO:0042802]; magnesium ion binding [GO:0000287]; tartronate-semialdehyde synthase activity [GO:0009028]; thiamine pyrophosphate binding [GO:0030976]
PF02775;PF00205;PF02776;
3.40.50.970;3.40.50.1220;
TPP enzyme family
null
null
CATALYTIC ACTIVITY: Reaction=2 glyoxylate + H(+) = 2-hydroxy-3-oxopropanoate + CO2; Xref=Rhea:RHEA:10136, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:36655, ChEBI:CHEBI:57978; EC=4.1.1.47;
null
PATHWAY: Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 2/4.
null
null
FUNCTION: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).
Escherichia coli (strain K12)
P0AEP9
GLCD_ECOLI
MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATD...
1.1.99.14
COFACTOR: Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
DNA damage response [GO:0006974]; glycolate catabolic process [GO:0046296]
glycolate oxidase complex [GO:0009339]; plasma membrane [GO:0005886]
(S)-2-hydroxy-acid oxidase activity [GO:0003973]; D-2-hydroxy-acid dehydrogenase activity [GO:0047809]; FAD binding [GO:0071949]; glycolate dehydrogenase activity [GO:0019154]
PF02913;PF01565;
3.30.465.10;3.30.70.2190;3.30.70.2740;3.30.43.10;
FAD-binding oxidoreductase/transferase type 4 family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:2689218}. Note=Glycolate oxidoreductase activity was shown to be firmly associated with the cytoplasmic membranes. {ECO:0000269|PubMed:2689218}.
CATALYTIC ACTIVITY: Reaction=A + glycolate = AH2 + glyoxylate; Xref=Rhea:RHEA:21264, ChEBI:CHEBI:13193, ChEBI:CHEBI:17499, ChEBI:CHEBI:29805, ChEBI:CHEBI:36655; EC=1.1.99.14; Evidence={ECO:0000269|PubMed:4557653, ECO:0000305|PubMed:8606183}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21265; Evidence={ECO:0000...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=40 uM for glycolate {ECO:0000269|PubMed:4557653}; KM=0.7 mM for D-lactate {ECO:0000269|PubMed:4557653}; Note=Parameters measured from a partially purified enzyme from extracts of glycolate grown cells. {ECO:0000305|PubMed:4557653};
null
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0-8.8. {ECO:0000269|PubMed:4557653};
null
FUNCTION: Component of a complex that catalyzes the oxidation of glycolate to glyoxylate (PubMed:4557653, PubMed:8606183). Is required for E.coli to grow on glycolate as a sole source of carbon (PubMed:8606183). Is also able to oxidize D-lactate ((R)-lactate) with a similar rate (PubMed:4557653). Does not link directly...
Escherichia coli (strain K12)
P0AER0
GLPF_ECOLI
MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAALVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILMGLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAGWGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSEQKASL
null
null
cellular response to mercury ion [GO:0071288]; glycerol transmembrane transport [GO:0015793]
plasma membrane [GO:0005886]
glycerol channel activity [GO:0015254]; glycerol transmembrane transporter activity [GO:0015168]; urea transmembrane transporter activity [GO:0015204]
PF00230;
1.20.1080.10;
MIP/aquaporin (TC 1.A.8) family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:11039922, ECO:0000269|PubMed:11964478, ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000269|PubMed:11039922, ECO:0000269|PubMed:11964478}.
CATALYTIC ACTIVITY: Reaction=glycerol(in) = glycerol(out); Xref=Rhea:RHEA:29675, ChEBI:CHEBI:17754; Evidence={ECO:0000269|PubMed:11039922, ECO:0000269|PubMed:11226336, ECO:0000269|PubMed:6998951, ECO:0000269|PubMed:7512955};
null
null
null
null
FUNCTION: Mediates glycerol diffusion across the cytoplasmic membrane via a pore-type mechanism (PubMed:11039922, PubMed:11226336, PubMed:6998951, PubMed:7512955). Is highly permeable to glycerol, but less well permeated by water (PubMed:11226336). Does not transport ions (PubMed:7512955). It may also have limited perm...
Escherichia coli (strain K12)
P0AES0
GSP_ECOLI
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC...
3.5.1.78; 6.3.1.8
null
glutathione metabolic process [GO:0006749]; spermidine metabolic process [GO:0008216]
cytosol [GO:0005829]
ATP binding [GO:0005524]; glutathionylspermidine amidase activity [GO:0008884]; glutathionylspermidine synthase activity [GO:0008885]; metal ion binding [GO:0046872]
PF05257;PF03738;
3.30.1490.330;3.90.1720.10;
Glutathionylspermidine synthase preATP-grasp family
PTM: Oxidation of Cys-59 to sulfenic acid during oxidative stress selectively inhibits the amidase activity which leads to a rapid increase in the amounts of intracellular Gsp and Gsp S-thiolated proteins (GspSSPs). {ECO:0000269|PubMed:20530482}.
null
CATALYTIC ACTIVITY: Reaction=ATP + glutathione + spermidine = ADP + glutathionylspermidine + H(+) + phosphate; Xref=Rhea:RHEA:21272, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57834, ChEBI:CHEBI:57835, ChEBI:CHEBI:57925, ChEBI:CHEBI:456216; EC=6.3.1.8; Evidence={ECO:0000269|PubMed:20530482, EC...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=100 uM for ATP (at pH 6.8) {ECO:0000269|PubMed:17124497, ECO:0000269|PubMed:7775463}; KM=800 uM for glutathione (at pH 6.8) {ECO:0000269|PubMed:17124497, ECO:0000269|PubMed:7775463}; KM=218 uM for glutathione {ECO:0000269|PubMed:17124497, ECO:0000269|PubMed:7775463...
PATHWAY: Sulfur metabolism; glutathione metabolism.; PATHWAY: Amine and polyamine metabolism; spermidine metabolism.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is around 6.8 for Gsp synthetase activity. {ECO:0000269|PubMed:7775463};
null
FUNCTION: Catalyzes the formation of an amide bond between glutathione (GSH) and spermidine coupled with hydrolysis of ATP; also catalyzes the opposing reaction, i.e. the hydrolysis of glutathionylspermidine (Gsp) back to glutathione and spermidine. The amidase active site can also hydrolyze Gsp-disulfide (Gsp-S-S-Gsp)...
Escherichia coli (strain K12)
P0AES1
GSP_SHIFL
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQWTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLENKGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFC...
3.5.1.78; 6.3.1.8
null
glutathione metabolic process [GO:0006749]; spermidine metabolic process [GO:0008216]
null
ATP binding [GO:0005524]; glutathionylspermidine amidase activity [GO:0008884]; glutathionylspermidine synthase activity [GO:0008885]; metal ion binding [GO:0046872]
PF05257;PF03738;
3.30.1490.330;3.90.1720.10;
Glutathionylspermidine synthase preATP-grasp family
PTM: Oxidation of Cys-59 to sulfenic acid during oxidative stress selectively inhibits the amidase activity. {ECO:0000250|UniProtKB:P0AES0}.
null
CATALYTIC ACTIVITY: Reaction=ATP + glutathione + spermidine = ADP + glutathionylspermidine + H(+) + phosphate; Xref=Rhea:RHEA:21272, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57834, ChEBI:CHEBI:57835, ChEBI:CHEBI:57925, ChEBI:CHEBI:456216; EC=6.3.1.8; Evidence={ECO:0000250|UniProtKB:P0AES0}; ...
null
PATHWAY: Sulfur metabolism; glutathione metabolism.; PATHWAY: Amine and polyamine metabolism; spermidine metabolism.
null
null
FUNCTION: Catalyzes the formation of an amide bond between glutathione (GSH) and spermidine coupled with hydrolysis of ATP; also catalyzes the opposing reaction, i.e. the hydrolysis of glutathionylspermidine (Gsp) back to glutathione and spermidine. May act synergistically with glutaredoxin to regulate the redox enviro...
Shigella flexneri
P0AES2
GUDD_ECOLI
MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIKDNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQTFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKATPLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAESIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMAEFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQ...
4.2.1.40
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:10769114};
D-glucarate catabolic process [GO:0042838]
null
glucarate dehydratase activity [GO:0008872]; magnesium ion binding [GO:0000287]
PF13378;
3.20.20.120;3.30.390.10;
Mandelate racemase/muconate lactonizing enzyme family, GlucD subfamily
null
null
CATALYTIC ACTIVITY: Reaction=D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O; Xref=Rhea:RHEA:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:30612, ChEBI:CHEBI:42819; EC=4.2.1.40; Evidence={ECO:0000269|PubMed:9772162};
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=16 uM for idarate {ECO:0000269|PubMed:9772162}; KM=60 uM for glucarate {ECO:0000269|PubMed:9772162};
PATHWAY: Carbohydrate acid metabolism; D-glucarate degradation; 2,5-dioxopentanoate from D-glucarate: step 1/2.
null
null
FUNCTION: Catalyzes the dehydration of glucarate or L-idarate to 5-keto-4-deoxy-D-glucarate (5-kdGluc) (PubMed:9772162). Also catalyzes the epimerization of D-glucarate and L-idarate (PubMed:11513584). {ECO:0000269|PubMed:11513584, ECO:0000269|PubMed:9772162}.
Escherichia coli (strain K12)
P0AES4
GYRA_ECOLI
MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMPTKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFPTAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEKIAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNL...
5.6.2.2
null
DNA topological change [GO:0006265]; DNA-templated DNA replication [GO:0006261]; DNA-templated transcription [GO:0006351]; negative regulation of DNA-templated DNA replication [GO:2000104]; response to antibiotic [GO:0046677]; response to xenobiotic stimulus [GO:0009410]
chromosome [GO:0005694]; cytoplasm [GO:0005737]; cytosol [GO:0005829]; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex [GO:0009330]; membrane [GO:0016020]
ATP binding [GO:0005524]; ATP-dependent activity, acting on DNA [GO:0008094]; DNA binding [GO:0003677]; DNA negative supercoiling activity [GO:0034335]; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity [GO:0003918]; identical protein binding [GO:0042802]
PF03989;PF00521;
3.30.1360.40;2.120.10.90;3.90.199.10;1.10.268.10;
Type II topoisomerase GyrA/ParC subunit family
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01897}.
CATALYTIC ACTIVITY: Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-Rule:MF_01897, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760, ECO:0000269|PubMed:9278055};
null
null
null
null
FUNCTION: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to maintain chromosomes in an underwound state (PubMed:12051842, PubMed:18642932, PubMed:186775, PubMed:19060136, PubMed:19965760, PubMed:20356737, PubMed:22457353, PubMed:23294697, PubMed:30...
Escherichia coli (strain K12)
P0AES6
GYRB_ECOLI
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLI...
5.6.2.2
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000269|PubMed:12051843, ECO:0000269|PubMed:18642932}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000269|PubMed:12051843, ECO:0000269|PubMed:18642932}; Name=Ca(2+); Xref=ChEBI:CHEBI:29...
DNA topological change [GO:0006265]; DNA-templated DNA replication [GO:0006261]; DNA-templated transcription [GO:0006351]; response to antibiotic [GO:0046677]; response to xenobiotic stimulus [GO:0009410]
chromosome [GO:0005694]; cytoplasm [GO:0005737]; cytosol [GO:0005829]; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex [GO:0009330]
ATP binding [GO:0005524]; ATP-dependent activity, acting on DNA [GO:0008094]; DNA binding [GO:0003677]; DNA negative supercoiling activity [GO:0034335]; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity [GO:0003918]; metal ion binding [GO:0046872]
PF00204;PF00986;PF21249;PF18053;PF02518;PF01751;
3.10.20.690;3.30.230.10;3.40.50.670;3.30.565.10;
Type II topoisomerase family
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01898}.
CATALYTIC ACTIVITY: Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:12051843, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760, ECO:0000269|PubMed:206...
null
null
null
null
FUNCTION: DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner to maintain chromosomes in an underwound state (PubMed:12051842, PubMed:12051843, PubMed:1323022, PubMed:18642932, PubMed:186775, PubMed:19060136, PubMed:19965760, PubMed:20356737, PubMed:20675723, PubMed:22457353,...
Escherichia coli (strain K12)
P0AES9
HDEA_ECOLI
MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLAVDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGEWDKIKKDM
null
null
cellular stress response to acidic pH [GO:1990451]; chaperone-mediated protein folding [GO:0061077]
outer membrane-bounded periplasmic space [GO:0030288]
identical protein binding [GO:0042802]; protein folding chaperone [GO:0044183]; protein homodimerization activity [GO:0042803]; unfolded protein binding [GO:0051082]
PF06411;
1.10.890.10;
HdeA family
null
SUBCELLULAR LOCATION: Periplasm {ECO:0000255|HAMAP-Rule:MF_00946, ECO:0000269|PubMed:17085547}.
null
null
null
null
null
FUNCTION: Required for optimal acid stress protection. Exhibits a chaperone-like activity only at pH below 3 by suppressing non-specifically the aggregation of denaturated periplasmic proteins. Important for survival of enteric bacteria in the acidic environment of the host stomach. Also promotes the solubilization at ...
Escherichia coli (strain K12)
P0AET2
HDEB_ECOLI
MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFIDLNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQASNDLPN
null
null
cellular stress response to acidic pH [GO:1990451]; response to acidic pH [GO:0010447]
outer membrane-bounded periplasmic space [GO:0030288]
unfolded protein binding [GO:0051082]
PF06411;
1.10.890.10;
HdeB family
null
SUBCELLULAR LOCATION: Periplasm {ECO:0000255|HAMAP-Rule:MF_00947, ECO:0000269|PubMed:17085547}.
null
null
null
null
null
FUNCTION: Required for optimal acid stress protection, which is important for survival of enteric bacteria in the acidic environment of the host stomach. Exhibits a chaperone-like activity at acidic pH by preventing the aggregation of many different periplasmic proteins. {ECO:0000255|HAMAP-Rule:MF_00947, ECO:0000269|Pu...
Escherichia coli (strain K12)
P0AET8
HDHA_ECOLI
MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN
1.1.1.159
null
bile acid catabolic process [GO:0030573]; lipid catabolic process [GO:0016042]
cytosol [GO:0005829]; protein-containing complex [GO:0032991]
chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity [GO:0106281]; cholate 7-alpha-dehydrogenase activity [GO:0008709]; identical protein binding [GO:0042802]; NAD binding [GO:0051287]
PF13561;
3.40.50.720;
Short-chain dehydrogenases/reductases (SDR) family
null
null
CATALYTIC ACTIVITY: Reaction=cholate + NAD(+) = 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + H(+) + NADH; Xref=Rhea:RHEA:19409, ChEBI:CHEBI:11893, ChEBI:CHEBI:15378, ChEBI:CHEBI:29747, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.159; Evidence={ECO:0000269|PubMed:2007545, ECO:0000269|PubMed:9722677}; Physiologica...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.19 mM for taurochenodeoxycholate {ECO:0000269|PubMed:2007545}; KM=0.43 mM for chenodeoxycholate {ECO:0000269|PubMed:2007545}; KM=1.2 mM for cholate {ECO:0000269|PubMed:2007545}; KM=1.25 mM for glycocholate {ECO:0000269|PubMed:2007545}; KM=2 mM for taurocholate {E...
null
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.5 (PubMed:2007545). The enzyme is stable between pH 8 and 9 (PubMed:2007545). {ECO:0000269|PubMed:2007545};
null
FUNCTION: 7alpha-hydroxysteroid dehydrogenase involved in the metabolism of bile acids. Catalyzes the NAD(+)-dependent oxidation of the 7alpha-hydroxy group of 7alpha-hydroxysteroids, such as the major human bile acids cholate and chenodeoxycholate, to the corresponding 7-oxosteroids. To a lesser extent, can also act o...
Escherichia coli (strain K12)
P0AEU7
SKP_ECOLI
MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSNTLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFEQDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQVK
null
null
chaperone-mediated protein folding [GO:0061077]; Gram-negative-bacterium-type cell outer membrane assembly [GO:0043165]; protein folding [GO:0006457]; protein insertion into membrane from inner side [GO:0032978]; protein maturation by protein folding [GO:0022417]; protein stabilization [GO:0050821]
cytosol [GO:0005829]; outer membrane-bounded periplasmic space [GO:0030288]
identical protein binding [GO:0042802]; lipopolysaccharide binding [GO:0001530]; unfolded protein binding [GO:0051082]
PF03938;
3.30.910.20;
Skp family
null
SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:1838129, ECO:0000269|PubMed:8730870}.
null
null
null
null
null
FUNCTION: Molecular chaperone that interacts specifically with outer membrane proteins, thus maintaining the solubility of early folding intermediates during passage through the periplasm. Required for the efficient release of OmpA from the inner membrane, the maintenance of its solubility in the periplasm, and, in ass...
Escherichia coli (strain K12)
P0AEV1
RSSB_ECOLI
MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDALELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGVEDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQELQPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALLLRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLILVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQ...
null
null
negative regulation of DNA-templated transcription [GO:0045892]; positive regulation of proteolysis [GO:0045862]; protein destabilization [GO:0031648]; regulation of DNA-templated transcription [GO:0006355]
cytosol [GO:0005829]; protein-DNA complex [GO:0032993]; sigma factor antagonist complex [GO:1903865]
phosphorelay response regulator activity [GO:0000156]; sigma factor antagonist activity [GO:0016989]; transcription cis-regulatory region binding [GO:0000976]
PF00072;
3.40.50.2300;3.60.40.10;
RssB family
PTM: Phosphorylated. Phosphorylation stimulates the interaction with RpoS and, therefore, the proteolysis of RpoS. {ECO:0000255|HAMAP-Rule:MF_00958, ECO:0000269|PubMed:10339606, ECO:0000269|PubMed:11442836, ECO:0000269|PubMed:24352426, ECO:0000269|PubMed:9515704}.
null
null
null
null
null
null
FUNCTION: Regulates the turnover of the sigma S factor (RpoS) by promoting its proteolysis in exponentially growing cells. Acts by binding and delivering RpoS to the ClpXP protease. RssB is not co-degraded with RpoS, but is released from the complex and can initiate a new cycle of RpoS recognition and degradation. In s...
Escherichia coli (strain K12)
P0AEV9
HYCI_ECOLI
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE
3.4.23.51
null
protein processing [GO:0016485]
null
aspartic-type endopeptidase activity [GO:0004190]; endopeptidase activity [GO:0004175]; enzyme activator activity [GO:0008047]; metal ion binding [GO:0046872]
PF01750;
3.40.50.1450;
Peptidase A31 family
null
null
CATALYTIC ACTIVITY: Reaction=This enzyme specifically removes a 32-amino acid peptide from the C-terminus of the precursor of the large subunit of E.coli hydrogenase 3 by cleavage at the C-terminal side of Arg-537.; EC=3.4.23.51; Evidence={ECO:0000269|PubMed:10727938};
null
null
null
null
FUNCTION: Protease involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3. {ECO:0000269|PubMed:10727938}.
Escherichia coli (strain K12)
P0AEW6
INGK_ECOLI
MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLVDIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNYSVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESGERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKKYNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALDWVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYE...
2.7.1.73
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_02246, ECO:0000269|PubMed:10879466, ECO:0000269|PubMed:7665468}; Note=Mg(2+) is probably required for activity in addition to its role in forming the Mg-ATP complex. {ECO:0000269|PubMed:10879466};
GMP salvage [GO:0032263]; IMP salvage [GO:0032264]; phosphorylation [GO:0016310]; purine ribonucleoside salvage [GO:0006166]
null
ATP binding [GO:0005524]; guanosine kinase activity [GO:0106366]; guanosine tetraphosphate binding [GO:0097216]; inosine kinase activity [GO:0008906]
PF00294;
3.40.1190.20;
Carbohydrate kinase PfkB family
null
null
CATALYTIC ACTIVITY: Reaction=ATP + guanosine = ADP + GMP + H(+); Xref=Rhea:RHEA:27710, ChEBI:CHEBI:15378, ChEBI:CHEBI:16750, ChEBI:CHEBI:30616, ChEBI:CHEBI:58115, ChEBI:CHEBI:456216; EC=2.7.1.73; Evidence={ECO:0000255|HAMAP-Rule:MF_02246, ECO:0000269|PubMed:10879466, ECO:0000269|PubMed:7665468}; PhysiologicalDirection=...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=6.1 uM for guanosine {ECO:0000269|PubMed:7665468}; KM=7.8 uM for guanosine {ECO:0000269|PubMed:10879466}; KM=2.1 mM for inosine {ECO:0000269|PubMed:7665468}; KM=1.5 mM for inosine {ECO:0000269|PubMed:10879466}; KM=4.3 mM for deoxyguanosine {ECO:0000269|PubMed:10879...
PATHWAY: Purine metabolism; IMP biosynthesis via salvage pathway; IMP from inosine: step 1/1. {ECO:0000255|HAMAP-Rule:MF_02246, ECO:0000305|PubMed:11440147}.; PATHWAY: Purine metabolism; GMP biosynthesis via salvage pathway. {ECO:0000255|HAMAP-Rule:MF_02246, ECO:0000305|PubMed:11440147}.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.2 for guanosine kinase activity. Optimum pH is 6.9 for inosine kinase activity. {ECO:0000269|PubMed:7665468};
BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 38 degrees Celsius for guanosine kinase activity and between 26 and 39 degrees Celsius for inosine kinase activity. {ECO:0000269|PubMed:7665468};
FUNCTION: Catalyzes the phosphorylation of guanosine and inosine to GMP and IMP, respectively (PubMed:10879466, PubMed:7665468, PubMed:7721718). Can also use deoxyguanosine and xanthosine, but not adenosine, uridine, cytidine or deoxythymidine (PubMed:10879466, PubMed:7665468). Shows a strong preference for guanosine (...
Escherichia coli (strain K12)
P0AEW9
K1PF_ECOLI
MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGINVAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGEVTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQCPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIAHVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN
2.7.1.56
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|Ref.6}; Note=Can also use Mn(2+) or Co(2+), with lower efficiency. {ECO:0000269|Ref.6};
fructose catabolic process [GO:0006001]
cytosol [GO:0005829]
1-phosphofructokinase activity [GO:0008662]; ATP binding [GO:0005524]; phosphofructokinase activity [GO:0008443]
PF00294;
3.40.1190.20;
Carbohydrate kinase PfkB family
null
null
CATALYTIC ACTIVITY: Reaction=ATP + beta-D-fructose 1-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+); Xref=Rhea:RHEA:14213, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32966, ChEBI:CHEBI:138881, ChEBI:CHEBI:456216; EC=2.7.1.56; Evidence={ECO:0000269|PubMed:10833389, ECO:0000269|Ref.6};
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.25 mM for fructose 1-phosphate {ECO:0000269|Ref.6}; KM=0.36 mM for fructose 1-phosphate (in the absence of KCl) {ECO:0000269|PubMed:10833389}; KM=0.125 mM for fructose 1-phosphate (in the presence of 50 mM KCl) {ECO:0000269|PubMed:10833389}; KM=0.12 mM for ATP {E...
null
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.8 in 50 mM Tris-HC1 and 8.5 in 50 mM NH(4)HCO(3). {ECO:0000269|Ref.6};
null
FUNCTION: Catalyzes the ATP-dependent phosphorylation of fructose-l-phosphate to fructose-l,6-bisphosphate (PubMed:10833389, Ref.6). Is specific for fructose-l-phosphate (Ref.6). GTP, UTP and CTP can also function as phosphoryl donors showing 60%, 20% and 10% of the activity of ATP (Ref.6). {ECO:0000269|PubMed:10833389...
Escherichia coli (strain K12)
P0AEX7
LIVH_ECOLI
MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVIWIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGILGRPEVEKV
null
null
branched-chain amino acid transport [GO:0015803]; D-alanine transmembrane transport [GO:0042941]; isoleucine transmembrane transport [GO:1903714]; L-alanine transport [GO:0015808]; L-isoleucine import across plasma membrane [GO:1903806]; L-valine transmembrane transport [GO:1903785]; leucine import across plasma membra...
ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing [GO:0055052]; membrane [GO:0016020]; plasma membrane [GO:0005886]
branched-chain amino acid transmembrane transporter activity [GO:0015658]; L-isoleucine transmembrane transporter activity [GO:0015188]; L-leucine transmembrane transporter activity [GO:0015190]; L-phenylalanine transmembrane transporter activity [GO:0015192]; L-valine transmembrane transporter activity [GO:0005304]
PF02653;
null
Binding-protein-dependent transport system permease family, LivHM subfamily
null
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
null
null
null
null
null
FUNCTION: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane.
Escherichia coli (strain K12)
P0AEX9
MALE_ECOLI
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVN...
null
null
carbohydrate transport [GO:0008643]; cell chemotaxis [GO:0060326]; detection of maltose stimulus [GO:0034289]; DNA damage response [GO:0006974]; maltodextrin transmembrane transport [GO:0042956]; maltose transport [GO:0015768]
ATP-binding cassette (ABC) transporter complex [GO:0043190]; ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing [GO:0055052]; maltose transport complex [GO:1990060]; membrane [GO:0016020]; outer membrane-bounded periplasmic space [GO:0030288]; periplasmic space [GO:0042597]
carbohydrate transmembrane transporter activity [GO:0015144]; maltose binding [GO:1901982]
PF01547;
3.40.190.10;
Bacterial solute-binding protein 1 family
null
SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:4215651}.
null
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.9 uM for maltose {ECO:0000269|PubMed:776623}; KM=2 uM for maltotriose {ECO:0000269|PubMed:776623};
null
null
null
FUNCTION: Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose. {ECO:0000269|PubMed:2026607, ECO:0000269|PubMed:2155217, ECO:0000269|PubMed:4215651, ECO:0000269|PubMed:776623}.
Escherichia coli (strain K12)
P0AEY3
MAZG_ECOLI
MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFADSSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWTTLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQKANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL
3.6.1.8
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
cellular response to starvation [GO:0009267]; dATP catabolic process [GO:0046061]; dGTP catabolic process [GO:0006203]; dTTP catabolic process [GO:0046076]; dUTP catabolic process [GO:0046081]; TTP catabolic process [GO:0046047]; UTP catabolic process [GO:0046052]
null
ATP binding [GO:0005524]; ATP diphosphatase activity [GO:0047693]; metal ion binding [GO:0046872]; nucleoside triphosphate diphosphatase activity [GO:0047429]
PF03819;
1.10.287.1080;
Nucleoside triphosphate pyrophosphohydrolase family
null
null
CATALYTIC ACTIVITY: Reaction=ATP + H2O = AMP + diphosphate + H(+); Xref=Rhea:RHEA:14245, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:456215; EC=3.6.1.8; Evidence={ECO:0000269|PubMed:12218018, ECO:0000269|PubMed:18353782};
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.34 mM for dATP (at pH 9.6 and at 37 degrees Celsius in the presence of 5 mM magnesium) {ECO:0000269|PubMed:20529853}; KM=0.71 mM for UTP (at pH 9.6 and at 37 degrees Celsius in the presence of 5 mM magnesium) {ECO:0000269|PubMed:20529853}; KM=0.36 mM for 8-oxo-dG...
null
null
null
FUNCTION: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the type II MazE-MazF toxin-antitoxin (TA) system which mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp produced by RelA under amino acid starvation...
Escherichia coli (strain K12)
P0AEY5
MDAB_ECOLI
MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRADSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRKDPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGMEPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG
1.6.5.10
COFACTOR: Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|PubMed:8611590};
null
cytosol [GO:0005829]
flavin adenine dinucleotide binding [GO:0050660]; NADPH dehydrogenase (quinone) activity [GO:0008753]
PF02525;
3.40.50.360;
Oxidoreductase MdaB family
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:8611590}.
CATALYTIC ACTIVITY: Reaction=a quinone + H(+) + NADPH = a quinol + NADP(+); Xref=Rhea:RHEA:46164, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132124; EC=1.6.5.10; Evidence={ECO:0000269|PubMed:8611590}; CATALYTIC ACTIVITY: Reaction=H(+) + menadione + NADPH = menadiol + NADP(+)...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=10.5 uM for NADPH {ECO:0000269|PubMed:8611590}; KM=6 uM for menadione {ECO:0000269|PubMed:8611590};
null
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:8611590};
null
FUNCTION: NADPH-specific quinone reductase (PubMed:8611590). Is most active with quinone derivatives and ferricyanide as electron acceptors (PubMed:8611590). Can use menadione, 1,4-naphthoquinone and 1,4-benzoquinone (PubMed:8611590). May work in concert with YgiN to form a quinone redox cycle (PubMed:15613473). {ECO:0...
Escherichia coli (strain K12)
P0AEY8
MDFA_ECOLI
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGL...
null
null
regulation of cellular pH [GO:0030641]; response to antibiotic [GO:0046677]; xenobiotic detoxification by transmembrane export across the plasma membrane [GO:1990961]
plasma membrane [GO:0005886]
potassium:proton antiporter activity [GO:0015386]; sodium:proton antiporter activity [GO:0015385]
PF07690;
1.20.1720.10;
Major facilitator superfamily, MdfA family
null
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
null
null
null
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.5. {ECO:0000269|PubMed:9644262};
null
FUNCTION: Efflux pump driven by the proton motive force. Confers resistance to a broad spectrum of chemically unrelated drugs. Confers resistance to a diverse group of cationic or zwitterionic lipophilic compounds such as ethidium bromide, tetraphenylphosphonium, rhodamine, daunomycin, benzalkonium, rifampicin, tetracy...
Escherichia coli (strain K12)
P0AEZ1
METF_ECOLI
MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGVRPGL
1.5.1.54
COFACTOR: Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|PubMed:10201405, ECO:0000269|PubMed:11371182, ECO:0000269|PubMed:14275142, ECO:0000269|PubMed:16114881, ECO:0000269|PubMed:16605249, ECO:0000269|PubMed:19610625, ECO:0000269|PubMed:9922232};
methionine biosynthetic process [GO:0009086]; tetrahydrofolate interconversion [GO:0035999]
cytosol [GO:0005829]; protein-containing complex [GO:0032991]
FAD binding [GO:0071949]; methylenetetrahydrofolate reductase (NAD(P)H) activity [GO:0004489]; methylenetetrahydrofolate reductase NADH activity [GO:0106312]; protein-folding chaperone binding [GO:0051087]
PF02219;
3.20.20.220;
Methylenetetrahydrofolate reductase family
null
null
CATALYTIC ACTIVITY: Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH; Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636, ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54; Evidence={ECO:0000269|PubMed:11371182, ECO:0000269|...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=13 uM for NADH (at pH 7.2 and 15 degrees Celsius) {ECO:0000269|PubMed:9922232}; KM=0.8 uM for (6R)-5,10-methylenetetrahydrofolate (at pH 7.2 and 15 degrees Celsius) {ECO:0000269|PubMed:9922232}; KM=17 uM for NADH (at pH 7.2 and 15 degrees Celsius) {ECO:0000269|PubM...
PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.; PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway. {ECO:0000305|PubMed:14275142}.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.3-6.4. {ECO:0000269|PubMed:14275142};
null
FUNCTION: Catalyzes the NADH-dependent reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate (PubMed:9922232). Is required to provide the methyl group necessary for methionine synthetase to convert homocysteine to methionine; the methyl group is given by 5-methyltetrahydrofolate. Can also use NADPH as...
Escherichia coli (strain K12)
P0AEZ3
MIND_ECOLI
MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLRNLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGVAKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGILASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG
null
null
cell division [GO:0051301]; chromosome segregation [GO:0007059]; division septum assembly [GO:0000917]; negative regulation of cell division [GO:0051782]
cell pole [GO:0060187]; cytoplasmic side of plasma membrane [GO:0009898]; cytosol [GO:0005829]; plasma membrane [GO:0005886]
ATP binding [GO:0005524]; ATP hydrolysis activity [GO:0016887]; identical protein binding [GO:0042802]
PF01656;
3.40.50.300;
ParA family, MinD subfamily
null
SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein.
null
null
null
null
null
FUNCTION: ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they matu...
Escherichia coli (strain K12)
P0AF06
MOTB_ECOLI
MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVMWLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPNIEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPMFRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRANASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHENAESQNEPVSALEKPEVAPQVSVPTMPSAEPR
null
null
bacterial-type flagellum-dependent cell motility [GO:0071973]; chemotaxis [GO:0006935]; proton transmembrane transport [GO:1902600]
bacterial-type flagellum [GO:0009288]; bacterial-type flagellum stator complex [GO:0120101]; plasma membrane [GO:0005886]
proton channel activity [GO:0015252]
PF13677;PF00691;
3.30.1330.60;
MotB family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:2447650}; Single-pass type II membrane protein {ECO:0000269|PubMed:2447650}. Note=The OmpA-like domain probably functions to anchor the complex to the cell wall.
null
null
null
null
null
FUNCTION: MotA and MotB comprise the stator element of the flagellar motor complex. Required for the rotation of the flagellar motor. Probably a linker that fastens the torque-generating machinery to the cell wall. Overexpression of this protein with MotA improves motility in a pdeH disruption, (a c-di-GMP phosphodiest...
Escherichia coli (strain K12)
P0AF12
MTNN_ECOLI
MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVALLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADVTAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAKIRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSSLMVESLVQKLAHG
3.2.2.9
null
L-methionine salvage from methylthioadenosine [GO:0019509]; L-methionine salvage from S-adenosylmethionine [GO:0019284]; purine deoxyribonucleoside catabolic process [GO:0046124]; toxic metabolite repair [GO:0110052]
cytosol [GO:0005829]
adenosylhomocysteine nucleosidase activity [GO:0008782]; identical protein binding [GO:0042802]; methylthioadenosine nucleosidase activity [GO:0008930]; protein homodimerization activity [GO:0042803]
PF01048;
3.40.50.1580;
PNP/UDP phosphorylase family, MtnN subfamily
null
null
CATALYTIC ACTIVITY: Reaction=H2O + S-adenosyl-L-homocysteine = adenine + S-(5-deoxy-D-ribos-5-yl)-L-homocysteine; Xref=Rhea:RHEA:17805, ChEBI:CHEBI:15377, ChEBI:CHEBI:16708, ChEBI:CHEBI:57856, ChEBI:CHEBI:58195; EC=3.2.2.9; Evidence={ECO:0000255|HAMAP-Rule:MF_01684, ECO:0000269|PubMed:16101288, ECO:0000269|PubMed:39119...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.4 uM for 5'-methylthioadenosine (at pH 7 and 37 degrees Celsius) {ECO:0000269|PubMed:3911944}; KM=0.8 uM for 5'-methylthioadenosine (at pH 7 and 22 degrees Celsius) {ECO:0000269|PubMed:16101288}; KM=4.3 uM for S-adenosylhomocysteine (at pH 7 and 37 degrees Celsiu...
PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. {ECO:0000255|HAMAP-Rule:MF_01684}.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Exhibits activity across a broad pH range. Enzyme activity is moderately improved under acidic conditions. {ECO:0000269|PubMed:8941345};
BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 37-45 degrees Celsius. Rapidly inactivated after exposure for 10 minutes at 55 degrees Celsius. {ECO:0000269|PubMed:8941345};
FUNCTION: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively (PubMed:16101288, PubMed:3911944). Also cleaves 5'-deoxyadenosine, a ...
Escherichia coli (strain K12)
P0AF16
MURJ_ECOLI
MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVAFKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAGMLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIPAFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPRINFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPSGVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALP...
null
null
cell wall organization [GO:0071555]; glycolipid translocation [GO:0034203]; lipid translocation [GO:0034204]; lipid-linked peptidoglycan transport [GO:0015836]; peptidoglycan biosynthetic process [GO:0009252]; regulation of cell shape [GO:0008360]
division septum [GO:0000935]; plasma membrane [GO:0005886]
cardiolipin binding [GO:1901612]; lipid-linked peptidoglycan transporter activity [GO:0015648]
PF03023;
null
MurJ/MviN family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-Rule:MF_02078, ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:23935042}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_02078, ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:23935042}.
null
null
PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. {ECO:0000255|HAMAP-Rule:MF_02078, ECO:0000269|PubMed:25013077}.
null
null
FUNCTION: Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane. {ECO:0000255|HAMAP-Rule:MF_02078, ECO:0000269|PubMed:18708495, ECO:0000269|PubMed:18832143, ECO:0000269|PubMed:25013077}.
Escherichia coli (strain K12)
P0AF18
NAGA_ECOLI
MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQRSLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITTSDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLCENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLYNAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATAPAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNL...
3.5.1.25
COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:16630633, ECO:0000269|PubMed:17567047, ECO:0000269|PubMed:17567048}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:16630633, ECO:0000269|PubMed:17567047, ECO:0000269|PubMed:17567048}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evi...
N-acetylglucosamine catabolic process [GO:0006046]; N-acetylneuraminate catabolic process [GO:0019262]; protein homotetramerization [GO:0051289]
protein-containing complex [GO:0032991]
identical protein binding [GO:0042802]; N-acetylgalactosamine-6-phosphate deacetylase activity [GO:0047419]; N-acetylglucosamine-6-phosphate deacetylase activity [GO:0008448]; zinc ion binding [GO:0008270]
PF01979;
3.20.20.140;2.30.40.10;
Metallo-dependent hydrolases superfamily, NagA family
null
null
CATALYTIC ACTIVITY: Reaction=H2O + N-acetyl-D-glucosamine 6-phosphate = acetate + D-glucosamine 6-phosphate; Xref=Rhea:RHEA:22936, ChEBI:CHEBI:15377, ChEBI:CHEBI:30089, ChEBI:CHEBI:57513, ChEBI:CHEBI:58725; EC=3.5.1.25; Evidence={ECO:0000269|PubMed:16630633, ECO:0000269|PubMed:17567047, ECO:0000269|PubMed:17567048, ECO...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.3 mM for N-acetyl-glucosamine 6-phosphate (at 30 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:17567047, ECO:0000269|PubMed:4861885, ECO:0000269|PubMed:9143339}; KM=0.8 mM for N-acetyl-glucosamine 6-phosphate (at 37 degrees Celsius and pH 8.0) {ECO:0000269|PubM...
PATHWAY: Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 4/5. {ECO:0000305|PubMed:2190615}.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:17567047, ECO:0000269|PubMed:4861885, ECO:0000269|PubMed:9143339};
null
FUNCTION: Involved in the first step in the biosynthesis of amino-sugar-nucleotides. Catalyzes the hydrolysis of the N-acetyl group of N-acetylglucosamine-6-phosphate (GlcNAc-6-P) to yield glucosamine 6-phosphate and acetate. In vitro, can also hydrolyze substrate analogs such as N-thioacetyl-D-glucosamine-6-phosphate,...
Escherichia coli (strain K12)
P0AF24
NAGD_ECOLI
MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNYPSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKAGFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALCAGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETILVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI
3.1.3.5
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:16430214, ECO:0000269|PubMed:16990279}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:16430214, ECO:0000269|PubMed:16990279}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:16430214, ECO:0000269|PubMed:169...
UMP catabolic process [GO:0046050]
cytosol [GO:0005829]
5'-nucleotidase activity [GO:0008253]; alkaline phosphatase activity [GO:0004035]; magnesium ion binding [GO:0000287]; phosphatase activity [GO:0016791]; phosphoglycolate phosphatase activity [GO:0008967]; XMP 5'-nucleosidase activity [GO:0106411]
PF13344;PF13242;
3.40.50.1000;
HAD-like hydrolase superfamily, NagD family
null
null
CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377, ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5; Evidence={ECO:0000269|PubMed:16430214};
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=160 uM for UMP (at pH 7 and at 25 degrees Celsius) {ECO:0000269|PubMed:16430214}; KM=400 uM for GMP (at pH 7 and at 25 degrees Celsius) {ECO:0000269|PubMed:16430214}; KM=840 uM for AMP (at pH 7 and at 25 degrees Celsius) {ECO:0000269|PubMed:16430214}; KM=840 uM for...
null
null
null
FUNCTION: Catalyzes the dephosphorylation of an unusually broad range of substrate including deoxyribo- and ribonucleoside tri-, di-, and monophosphates, as well as polyphosphate and glucose-1-P (Glu1P). {ECO:0000269|PubMed:16430214, ECO:0000269|PubMed:16990279}.
Escherichia coli (strain K12)
P0AF28
NARL_ECOLI
MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQGIELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRGADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRERDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF
null
null
nitrate assimilation [GO:0042128]; phosphorelay signal transduction system [GO:0000160]; regulation of DNA-templated transcription [GO:0006355]
cytosol [GO:0005829]; protein-DNA complex [GO:0032993]
ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA-binding transcription repressor activity [GO:0001217]; identical protein binding [GO:0042802]; transcription cis-regulatory region binding [GO:0000976]
PF00196;PF00072;
3.40.50.2300;1.10.10.10;
null
null
null
null
null
null
null
null
FUNCTION: This protein activates the expression of the nitrate reductase (narGHJI) and formate dehydrogenase-N (fdnGHI) operons and represses the transcription of the fumarate reductase (frdABCD) operon in response to a nitrate/nitrite induction signal transmitted by either the NarX or NarQ proteins.
Escherichia coli (strain K12)
P0AF32
NARV_ECOLI
MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQMLDKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLTLIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKLVGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRRYQIVRSRR
1.7.5.1
COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250}; Note=Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme. {ECO:0000250};
anaerobic electron transport chain [GO:0019645]; anaerobic respiration [GO:0009061]; nitrate assimilation [GO:0042128]; nitrate metabolic process [GO:0042126]
membrane [GO:0016020]; nitrate reductase complex [GO:0009325]; plasma membrane [GO:0005886]
electron transfer activity [GO:0009055]; heme binding [GO:0020037]; metal ion binding [GO:0046872]; nitrate reductase activity [GO:0008940]
PF02665;
1.20.950.20;
null
null
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CATALYTIC ACTIVITY: Reaction=a quinol + nitrate = a quinone + H2O + nitrite; Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
null
null
null
null
FUNCTION: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.
Escherichia coli (strain K12)
P0AF36
ZAPB_ECOLI
MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQNAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV
null
null
division septum assembly [GO:0000917]; FtsZ-dependent cytokinesis [GO:0043093]
cell division site [GO:0032153]; cytosol [GO:0005829]
identical protein binding [GO:0042802]
PF06005;
1.20.5.340;
ZapB family
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18394147}. Note=Localizes to the septum at mid-cell, in a FtsZ-like pattern.
null
null
null
null
null
FUNCTION: Non-essential, abundant cell division factor that is required for proper Z-ring formation. It is recruited early to the divisome by direct interaction with FtsZ, stimulating Z-ring assembly and thereby promoting cell division earlier in the cell cycle. Its recruitment to the Z-ring requires functional FtsA or...
Escherichia coli (strain K12)
P0AF52
GHXP_ECOLI
MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVIVVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLGQHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANGVGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGLIFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTIRAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVY...
null
null
guanine import across plasma membrane [GO:0098710]; hypoxanthine transport [GO:0035344]
plasma membrane [GO:0005886]
guanine transmembrane transporter activity [GO:0015208]
PF00860;
null
Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family, Azg-like subfamily
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:24214977}; Multi-pass membrane protein {ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:24214977}.
null
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.6 uM for guanine {ECO:0000269|PubMed:24214977}; KM=11.2 uM for hypoxanthine {ECO:0000269|PubMed:24214977}; Vmax=4 nmol/min/mg enzyme with guanine as substrate {ECO:0000269|PubMed:24214977}; Vmax=2.7 nmol/min/mg enzyme with hypoxanthine as substrate {ECO:0000269|P...
null
null
null
FUNCTION: High-affinity transporter for guanine and hypoxanthine. {ECO:0000269|PubMed:24214977}.
Escherichia coli (strain K12)
P0AF61
GHOS_ECOLI
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK
3.1.-.-
null
null
null
RNA endonuclease activity [GO:0004521]
PF11080;
3.30.70.2360;
null
PTM: Unlike other TA proteinaceous antitoxins, this protein is stable with and without cellular stress; its structure has been determined in the absence of GhoT toxin. {ECO:0000269|PubMed:22941047}.
null
null
null
null
null
null
FUNCTION: Antitoxin component of a type V toxin-antitoxin (TA) system. Neutralizes the toxic effects of toxin GhoT by digesting ghoT transcripts in a sequence-specific manner (PubMed:22941047). In concert with GhoT is involved in reducing cell growth during antibacterial stress (PubMed:24373067). Overexpression leads t...
Escherichia coli (strain K12)
P0AF67
TSAE_ECOLI
MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG
null
null
maintenance of translational fidelity [GO:1990145]; tRNA threonylcarbamoyladenosine modification [GO:0002949]
cytoplasm [GO:0005737]; EKC/KEOPS complex [GO:0000408]
ADP binding [GO:0043531]; ATP binding [GO:0005524]; ATP hydrolysis activity [GO:0016887]; identical protein binding [GO:0042802]; metal ion binding [GO:0046872]; protein kinase activity [GO:0004672]
PF02367;
3.40.50.300;
TsaE family
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19376873}.
null
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=169 uM for ATP {ECO:0000269|PubMed:19376873}; Note=kcat is 0.0026 sec(-1) for ATP hydrolysis.;
null
null
null
FUNCTION: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems...
Escherichia coli (strain K12)
P0AF90
RRAB_ECOLI
MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLETLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAEKFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH
null
null
mRNA catabolic process [GO:0006402]; regulation of endoribonuclease activity [GO:0060699]
cytosol [GO:0005829]
endoribonuclease inhibitor activity [GO:0060698]; enzyme binding [GO:0019899]; protein homodimerization activity [GO:0042803]; ribonuclease inhibitor activity [GO:0008428]
PF06877;
3.30.70.970;
RraB family
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01888}.
null
null
null
null
null
FUNCTION: Globally modulates RNA abundance by binding to RNase E (Rne) and regulating its endonucleolytic activity. Can modulate Rne action in a substrate-dependent manner by altering the composition of the degradosome. {ECO:0000255|HAMAP-Rule:MF_01888, ECO:0000269|PubMed:16771842, ECO:0000269|PubMed:18510556}.
Escherichia coli (strain K12)
P0AF93
RIDA_ECOLI
MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPADVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATFPARSCVEVARLPKDVKIEIEAIAVRR
3.5.99.10
null
amino acid biosynthetic process [GO:0008652]; isoleucine biosynthetic process [GO:0009097]; organonitrogen compound catabolic process [GO:1901565]; protein homotrimerization [GO:0070207]; response to toxic substance [GO:0009636]
cytosol [GO:0005829]; membrane [GO:0016020]; protein-containing complex [GO:0032991]
2-iminobutanoate deaminase activity [GO:0120242]; 2-iminopropanoate deaminase activity [GO:0120243]; deaminase activity [GO:0019239]; identical protein binding [GO:0042802]; unfolded protein binding [GO:0051082]
PF01042;
3.30.1330.40;
RutC family
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CATALYTIC ACTIVITY: Reaction=2-iminobutanoate + H2O = 2-oxobutanoate + NH4(+); Xref=Rhea:RHEA:39975, ChEBI:CHEBI:15377, ChEBI:CHEBI:16763, ChEBI:CHEBI:28938, ChEBI:CHEBI:76545; EC=3.5.99.10; Evidence={ECO:0000250|UniProtKB:Q7CP78}; CATALYTIC ACTIVITY: Reaction=2-iminopropanoate + H2O = NH4(+) + pyruvate; Xref=Rhea:RHEA...
null
PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine. {ECO:0000250|UniProtKB:Q7CP78}.
null
null
FUNCTION: Accelerates the release of ammonia from reactive enamine/imine intermediates of the PLP-dependent threonine dehydratase (IlvA) in the low water environment of the cell. It catalyzes the deamination of enamine/imine intermediates to yield 2-ketobutyrate and ammonia. It is required for the detoxification of rea...
Escherichia coli (strain K12)
P0AFA2
NARX_ECOLI
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGLQDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMAVFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQNLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGV...
2.7.13.3
null
cellular response to nitrate [GO:0071249]; cellular response to nitrite [GO:0071250]; DNA damage response [GO:0006974]; nitrate assimilation [GO:0042128]; signal transduction [GO:0007165]
outer membrane-bounded periplasmic space [GO:0030288]; plasma membrane [GO:0005886]
ATP binding [GO:0005524]; phosphoprotein phosphatase activity [GO:0004721]; phosphorelay sensor kinase activity [GO:0000155]; protein homodimerization activity [GO:0042803]
PF00672;PF02518;PF07730;PF13675;
1.20.5.1930;6.10.340.10;1.20.120.960;3.30.565.10;
null
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000269|PubMed:15919996}.
CATALYTIC ACTIVITY: Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.; EC=2.7.13.3;
null
null
null
null
FUNCTION: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the NarL protein and of both nitrate/nitrite to the NarP protein. NarX probably activates NarL and NarP by phosphorylation in the presence of nitrate. NarX also plays a negative role in controlling NarL activity, probably th...
Escherichia coli (strain K12)
P0AFA7
NHAB_ECOLI
MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVGFYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKVREVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEV...
null
null
response to lithium ion [GO:0010226]
plasma membrane [GO:0005886]
sodium:proton antiporter activity [GO:0015385]
PF06450;
null
NhaB Na(+)/H(+) (TC 2.A.34) antiporter family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01599}.
CATALYTIC ACTIVITY: Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out); Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101; Evidence={ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000269|PubMed:7929345}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248; Evidence={ECO:0000255|HAMAP-Rule:MF_01599, E...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=16.66 mM for Na(+) (at pH 7.2) {ECO:0000269|PubMed:7929345}; KM=3.44 mM for Na(+) (at pH 7.6) {ECO:0000269|PubMed:7929345}; KM=1.53 mM for Na(+) (at pH 8.5) {ECO:0000269|PubMed:7929345}; KM=0.73 mM for Na(+) {ECO:0000269|PubMed:8019504}; KM=0.63 mM for Li(+) {ECO:0...
null
null
null
FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons (PubMed:1317851, PubMed:7929345, PubMed:8019504, PubMed:8093613). Catalyzes the exchange of 3 H(+) per 2 Na(+) (PubMed:7929345). Can also transport lithium (PubMed:8019504). Essential for regulation of intracellular pH under alkaline ...
Escherichia coli (strain K12)
P0AFB1
NLPI_ECOLI
MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVILARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYLGIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDDPNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMERLKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALLELSLLGQDQDDLAESDQQ
null
null
cell cycle [GO:0007049]; cell division [GO:0051301]; peptidoglycan metabolic process [GO:0000270]; receptor-mediated virion attachment to host cell [GO:0046813]
cell outer membrane [GO:0009279]; plasma membrane [GO:0005886]
protein homodimerization activity [GO:0042803]; protein-macromolecule adaptor activity [GO:0030674]
PF13432;
1.25.40.10;
null
null
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor.
null
null
null
null
null
FUNCTION: May be involved in cell division. May play a role in bacterial septation or regulation of cell wall degradation during cell division. Negatively controls the production of extracellular DNA (eDNA). {ECO:0000269|PubMed:10400590, ECO:0000269|PubMed:20833130}.
Escherichia coli (strain K12)
P0AFB5
NTRB_ECOLI
MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALPDPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNVRLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIAR...
2.7.13.3; 3.1.3.-
null
nitrogen fixation [GO:0009399]; regulation of DNA-templated transcription [GO:0006355]; regulation of nitrogen compound metabolic process [GO:0051171]; signal transduction involved in regulation of gene expression [GO:0023019]
cytoplasm [GO:0005737]
ATP binding [GO:0005524]; identical protein binding [GO:0042802]; phosphoprotein phosphatase activity [GO:0004721]; phosphorelay sensor kinase activity [GO:0000155]; phosphotransferase activity, carboxyl group as acceptor [GO:0016774]; protein histidine kinase activity [GO:0004673]; protein homodimerization activity [G...
PF02518;PF00512;PF00989;
1.10.287.130;3.30.565.10;3.30.450.20;
null
PTM: Autophosphorylated. {ECO:0000269|PubMed:10074086, ECO:0000269|PubMed:2016302, ECO:0000269|PubMed:2574599}.
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:15157101}.
CATALYTIC ACTIVITY: Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.; EC=2.7.13.3; Evidence={ECO:0000269|PubMed:10074086, ECO:0000269|PubMed:2574599};
null
null
null
null
FUNCTION: Member of the two-component regulatory system NtrB/NtrC, which controls expression of the nitrogen-regulated (ntr) genes in response to nitrogen limitation. Under conditions of nitrogen limitation, NtrB autophosphorylates and transfers the phosphoryl group to NtrC. In the presence of nitrogen, acts as a phosp...
Escherichia coli (strain K12)
P0AFB8
NTRC_ECOLI
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR...
null
null
nitrogen fixation [GO:0009399]; regulation of DNA-templated transcription [GO:0006355]; regulation of nitrogen utilization [GO:0006808]
cytosol [GO:0005829]
ATP binding [GO:0005524]; ATP hydrolysis activity [GO:0016887]; phosphorelay response regulator activity [GO:0000156]; sequence-specific DNA binding [GO:0043565]
PF02954;PF00072;PF00158;
1.10.8.60;3.40.50.2300;1.10.10.60;3.40.50.300;
null
PTM: Phosphorylated and dephosphorylated by NtrB (PubMed:2574599, PubMed:2874557). Phosphorylation induces strong cooperative binding to DNA (PubMed:1350679). {ECO:0000269|PubMed:1350679, ECO:0000269|PubMed:2574599, ECO:0000269|PubMed:2874557}.
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
null
null
null
null
null
FUNCTION: Member of the two-component regulatory system NtrB/NtrC, which controls expression of the nitrogen-regulated (ntr) genes in response to nitrogen limitation. Phosphorylated NtrC binds directly to DNA and stimulates the formation of open promoter-sigma54-RNA polymerase complexes (PubMed:1350679, PubMed:2574599,...
Escherichia coli (strain K12)
P0AFC0
NUDB_ECOLI
MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA
3.6.1.67
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:8798731}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000269|PubMed:8798731};
folic acid biosynthetic process [GO:0046656]; tetrahydrofolate biosynthetic process [GO:0046654]
null
dATP diphosphatase activity [GO:0008828]; dihydroneopterin triphosphate pyrophosphohydrolase activity [GO:0019177]; magnesium ion binding [GO:0000287]; metal ion binding [GO:0046872]
PF00293;
3.90.79.10;
Nudix hydrolase family
null
null
CATALYTIC ACTIVITY: Reaction=7,8-dihydroneopterin 3'-triphosphate + H2O = 7,8-dihydroneopterin 3'-phosphate + diphosphate + H(+); Xref=Rhea:RHEA:25302, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58462, ChEBI:CHEBI:58762; EC=3.6.1.67; Evidence={ECO:0000269|PubMed:8798731};
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.27 mM for dihydroneopterin triphosphate {ECO:0000269|PubMed:17698004, ECO:0000269|PubMed:8798731}; KM=0.79 mM for dATP {ECO:0000269|PubMed:17698004, ECO:0000269|PubMed:8798731};
null
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.5-9. {ECO:0000269|PubMed:17698004, ECO:0000269|PubMed:8798731};
null
FUNCTION: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP. {ECO:0000269|PubMed:17698004, ECO:0000269|PubMed:8798731}.
Escherichia coli (strain K12)
P0AFC3
NUOA_ECOLI
MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARARSKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVEAAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR
7.1.1.-
null
respiratory electron transport chain [GO:0022904]
membrane [GO:0016020]; NADH dehydrogenase complex [GO:0030964]; plasma membrane [GO:0005886]; plasma membrane respiratory chain complex I [GO:0045272]; respiratory chain complex I [GO:0045271]
NADH dehydrogenase (ubiquinone) activity [GO:0008137]; quinone binding [GO:0048038]
PF00507;
1.20.58.1610;
Complex I subunit 3 family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-Rule:MF_01394, ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01394, ECO:0000269|PubMed:15919996}.
CATALYTIC ACTIVITY: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; Evidence={ECO:0000255|HAMAP-Rule:MF_01394};
null
null
null
null
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ...
Escherichia coli (strain K12)
P0AFC7
NUOB_ECOLI
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLNDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPDEI
7.1.1.-
COFACTOR: Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000255|HAMAP-Rule:MF_01356}; Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01356};
aerobic respiration [GO:0009060]; electron transport coupled proton transport [GO:0015990]; respiratory electron transport chain [GO:0022904]
membrane [GO:0016020]; NADH dehydrogenase complex [GO:0030964]; plasma membrane [GO:0005886]; plasma membrane respiratory chain complex I [GO:0045272]; respiratory chain complex I [GO:0045271]
4 iron, 4 sulfur cluster binding [GO:0051539]; iron ion binding [GO:0005506]; NADH dehydrogenase (ubiquinone) activity [GO:0008137]; quinone binding [GO:0048038]
PF01058;
3.40.50.12280;
Complex I 20 kDa subunit family
null
SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
CATALYTIC ACTIVITY: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; Evidence={ECO:0000255|HAMAP-Rule:MF_01356};
null
null
null
null
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are transl...
Escherichia coli (strain K12)
P0AFD1
NUOE_ECOLI
MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPELLERYK
7.1.1.-
COFACTOR: Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000305}; Note=Binds 1 [2Fe-2S] cluster. {ECO:0000305};
respiratory electron transport chain [GO:0022904]; transmembrane transport [GO:0055085]
membrane [GO:0016020]; NADH dehydrogenase complex [GO:0030964]; plasma membrane [GO:0005886]; plasma membrane respiratory chain complex I [GO:0045272]; respiratory chain complex I [GO:0045271]
2 iron, 2 sulfur cluster binding [GO:0051537]; metal ion binding [GO:0046872]; NADH dehydrogenase activity [GO:0003954]; quinone binding [GO:0048038]
PF01257;
3.40.30.10;1.10.10.1590;
Complex I 24 kDa subunit family
null
null
CATALYTIC ACTIVITY: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
null
null
null
null
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ...
Escherichia coli (strain K12)
P0AFD4
NUOH_ECOLI
MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQNRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVPVSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFLGLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAEQELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTAFFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVI...
7.1.1.-
null
aerobic respiration [GO:0009060]; respiratory electron transport chain [GO:0022904]; transmembrane transport [GO:0055085]
membrane [GO:0016020]; NADH dehydrogenase complex [GO:0030964]; plasma membrane [GO:0005886]; plasma membrane respiratory chain complex I [GO:0045272]; respiratory chain complex I [GO:0045271]
NADH dehydrogenase activity [GO:0003954]; oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor [GO:0016655]; quinone binding [GO:0048038]
PF00146;
null
Complex I subunit 1 family
null
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CATALYTIC ACTIVITY: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
null
null
null
null
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ...
Escherichia coli (strain K12)
P0AFD6
NUOI_ECOLI
MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGKDKGEAENEAKPIDVKSLLP
7.1.1.-
COFACTOR: Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250}; Note=Binds 2 [4Fe-4S] clusters per subunit. {ECO:0000250};
aerobic respiration [GO:0009060]; respiratory electron transport chain [GO:0022904]
membrane [GO:0016020]; NADH dehydrogenase complex [GO:0030964]; plasma membrane [GO:0005886]; plasma membrane respiratory chain complex I [GO:0045272]; respiratory chain complex I [GO:0045271]
4 iron, 4 sulfur cluster binding [GO:0051539]; iron ion binding [GO:0005506]; NADH dehydrogenase (quinone) activity [GO:0050136]; quinone binding [GO:0048038]
PF12838;
3.30.70.3270;
Complex I 23 kDa subunit family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CATALYTIC ACTIVITY: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
null
null
null
null
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ...
Escherichia coli (strain K12)
P0AFE0
NUOJ_ECOLI
MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFFSLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAIMLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGREERAGEVLSNRKDDSAKRKTEEHA
7.1.1.-
null
respiratory electron transport chain [GO:0022904]
membrane [GO:0016020]; NADH dehydrogenase complex [GO:0030964]; plasma membrane [GO:0005886]; plasma membrane respiratory chain complex I [GO:0045272]; respiratory chain complex I [GO:0045271]
NADH dehydrogenase (ubiquinone) activity [GO:0008137]; quinone binding [GO:0048038]
PF00499;
1.20.120.1200;
Complex I subunit 6 family
null
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CATALYTIC ACTIVITY: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
null
null
null
null
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ...
Escherichia coli (strain K12)
P0AFE4
NUOK_ECOLI
MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASALAFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG
7.1.1.-
null
ATP synthesis coupled electron transport [GO:0042773]; respiratory electron transport chain [GO:0022904]
membrane [GO:0016020]; NADH dehydrogenase complex [GO:0030964]; plasma membrane [GO:0005886]; plasma membrane respiratory chain complex I [GO:0045272]; respiratory chain complex I [GO:0045271]
NADH dehydrogenase (ubiquinone) activity [GO:0008137]; quinone binding [GO:0048038]
PF00420;
1.10.287.3510;
Complex I subunit 4L family
null
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CATALYTIC ACTIVITY: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; Evidence={ECO:0000255|HAMAP-Rule:MF_01456};
null
null
null
null
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ...
Escherichia coli (strain K12)
P0AFE8
NUOM_ECOLI
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSV...
7.1.1.-
null
aerobic respiration [GO:0009060]; ATP synthesis coupled electron transport [GO:0042773]; electron transport coupled proton transport [GO:0015990]; respiratory electron transport chain [GO:0022904]
membrane [GO:0016020]; NADH dehydrogenase complex [GO:0030964]; plasma membrane [GO:0005886]; plasma membrane respiratory chain complex I [GO:0045272]; respiratory chain complex I [GO:0045271]
NADH dehydrogenase (ubiquinone) activity [GO:0008137]; ubiquinone binding [GO:0048039]
PF00361;
null
Complex I subunit 4 family
null
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CATALYTIC ACTIVITY: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
null
null
null
null
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ...
Escherichia coli (strain K12)
P0AFF0
NUON_ECOLI
MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIGLNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNKDEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYTILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKLSLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAIIAFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGE...
7.1.1.-
null
aerobic respiration [GO:0009060]; ATP synthesis coupled electron transport [GO:0042773]; electron transport coupled proton transport [GO:0015990]; respiratory electron transport chain [GO:0022904]
membrane [GO:0016020]; NADH dehydrogenase complex [GO:0030964]; plasma membrane [GO:0005886]; plasma membrane respiratory chain complex I [GO:0045272]; respiratory chain complex I [GO:0045271]
NADH dehydrogenase (ubiquinone) activity [GO:0008137]; quinone binding [GO:0048038]
PF00361;
null
Complex I subunit 2 family
null
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CATALYTIC ACTIVITY: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; Evidence={ECO:0000255|HAMAP-Rule:MF_00445};
null
null
null
null
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ...
Escherichia coli (strain K12)
P0AFF2
NUPC_ECOLI
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSN...
null
null
nucleoside transmembrane transport [GO:1901642]
plasma membrane [GO:0005886]
cytidine transmembrane transporter activity [GO:0015212]; nucleoside:proton symporter activity [GO:0015506]; uridine transmembrane transporter activity [GO:0015213]
PF07670;PF07662;PF01773;
null
Concentrative nucleoside transporter (CNT) (TC 2.A.41) family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:8022285}; Multi-pass membrane protein {ECO:0000255}.
CATALYTIC ACTIVITY: Reaction=adenosine(in) + H(+)(in) = adenosine(out) + H(+)(out); Xref=Rhea:RHEA:29987, ChEBI:CHEBI:15378, ChEBI:CHEBI:16335; Evidence={ECO:0000269|PubMed:14668133}; CATALYTIC ACTIVITY: Reaction=H(+)(in) + uridine(in) = H(+)(out) + uridine(out); Xref=Rhea:RHEA:29951, ChEBI:CHEBI:15378, ChEBI:CHEBI:167...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.6 uM for uridine (at pH 5.5) {ECO:0000269|PubMed:14668133}; KM=10 uM for uridine (at pH 6.5) {ECO:0000269|PubMed:14668133}; KM=15 uM for uridine (at pH 7.5) {ECO:0000269|PubMed:14668133}; KM=44 uM for uridine (at pH 8.5) {ECO:0000269|PubMed:14668133}; KM=1.6 uM f...
null
null
null
FUNCTION: Nucleoside transport protein that can transport adenosine, uridine, thymidine, cytidine and deoxycytidine (PubMed:11466294, PubMed:14668133, PubMed:15678184, PubMed:374403). Shows weak activity with inosine and xanthosine (PubMed:11466294, PubMed:14668133). Transport is driven by a proton motive force (PubMed...
Escherichia coli (strain K12)
P0AFF4
NUPG_ECOLI
MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVYSSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILINSFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHMQLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSMLLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLSRYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGC...
null
null
adenosine transport [GO:0032238]; nucleoside transmembrane transport [GO:1901642]; organic substance transport [GO:0071702]; purine nucleoside transmembrane transport [GO:0015860]; pyrimidine nucleoside transport [GO:0015864]; uridine transport [GO:0015862]
plasma membrane [GO:0005886]
cytidine transmembrane transporter activity [GO:0015212]; nucleoside:proton symporter activity [GO:0015506]; pyrimidine nucleoside transmembrane transporter activity [GO:0015214]; uridine transmembrane transporter activity [GO:0015213]
PF03825;
1.20.1250.20;
Major facilitator superfamily, Nucleoside:H(+) symporter (NHS) (TC 2.A.1.10) family
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-Rule:MF_02049, ECO:0000269|PubMed:15513740, ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:3311747, ECO:0000269|PubMed:33640454}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_02049, ECO:0000269|PubMed:33640454}.
CATALYTIC ACTIVITY: Reaction=adenosine(in) + H(+)(in) = adenosine(out) + H(+)(out); Xref=Rhea:RHEA:29987, ChEBI:CHEBI:15378, ChEBI:CHEBI:16335; Evidence={ECO:0000269|PubMed:15513740, ECO:0000269|PubMed:374403}; CATALYTIC ACTIVITY: Reaction=H(+)(in) + uridine(in) = H(+)(out) + uridine(out); Xref=Rhea:RHEA:29951, ChEBI:C...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=23.6 uM for uridine {ECO:0000269|PubMed:15513740}; KM=20.6 uM for adenosine {ECO:0000269|PubMed:15513740}; Vmax=67.2 nmol/min/mg enzyme with uridine as substrate {ECO:0000269|PubMed:15513740}; Vmax=56.8 nmol/min/mg enzyme with adenosine as substrate {ECO:0000269|Pu...
null
null
null
FUNCTION: Broad-specificity transporter of purine and pyrimidine nucleosides (PubMed:11466294, PubMed:15513740, PubMed:15678184, PubMed:3311747, PubMed:374403). Can transport adenosine, uridine, thymidine, cytidine, deoxycytidine, guanosine and inosine (PubMed:11466294, PubMed:15513740, PubMed:15678184, PubMed:3311747,...
Escherichia coli (strain K12)
P0AFF6
NUSA_ECOLI
MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGR...
null
null
DNA-templated transcription termination [GO:0006353]; protein complex oligomerization [GO:0051259]; regulation of DNA-templated transcription elongation [GO:0032784]; ribosome biogenesis [GO:0042254]; transcription antitermination [GO:0031564]
cytosol [GO:0005829]; transcription elongation factor complex [GO:0008023]
bacterial-type RNA polymerase core enzyme binding [GO:0001000]; DNA-binding transcription factor activity [GO:0003700]; nucleotide binding [GO:0000166]; protein domain specific binding [GO:0019904]; RNA binding [GO:0003723]
PF14520;PF13184;PF08529;PF00575;
3.30.300.20;1.10.150.20;2.40.50.140;3.30.1480.10;
NusA family
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945, ECO:0000269|PubMed:17272300}. Note=Colocalizes with nucleoids.
null
null
null
null
null
FUNCTION: Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions...
Escherichia coli (strain K12)
P0AFG3
ODO1_ECOLI
MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRSTFQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFRGHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEALKQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLGAKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQDLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNP...
1.2.4.2
COFACTOR: Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000305|PubMed:17367808};
tricarboxylic acid cycle [GO:0006099]
cytoplasm [GO:0005737]; cytosol [GO:0005829]; oxoglutarate dehydrogenase complex [GO:0045252]
identical protein binding [GO:0042802]; magnesium ion binding [GO:0000287]; nucleotide binding [GO:0000166]; oxoglutarate dehydrogenase (succinyl-transferring) activity [GO:0004591]; thiamine pyrophosphate binding [GO:0030976]
PF16078;PF00676;PF16870;PF02779;
3.40.50.12470;3.40.50.970;3.40.50.11610;1.10.287.1150;
Alpha-ketoglutarate dehydrogenase family
null
null
CATALYTIC ACTIVITY: Reaction=2-oxoglutarate + H(+) + N(6)-[(R)-lipoyl]-L-lysyl-[dihydrolipoyllysine-residue succinyltransferase] = CO2 + N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[dihydrolipoyllysine-residue succinyltransferase]; Xref=Rhea:RHEA:12188, Rhea:RHEA-COMP:10483, Rhea:RHEA-COMP:10484, ChEBI:CHEBI:15378, C...
null
null
null
null
FUNCTION: E1 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the decarboxylation of 2-oxoglutarate, the first step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2). {ECO:0000269|PubMed:17367808}.
Escherichia coli (strain K12)
P0AFG6
ODO2_ECOLI
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGN...
2.3.1.61
COFACTOR: Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088; Note=Binds 1 lipoyl cofactor covalently.;
L-lysine catabolic process to acetyl-CoA via saccharopine [GO:0033512]; tricarboxylic acid cycle [GO:0006099]
cytoplasm [GO:0005737]; cytosol [GO:0005829]; oxoglutarate dehydrogenase complex [GO:0045252]
dihydrolipoyllysine-residue succinyltransferase activity [GO:0004149]; lipoic acid binding [GO:0031405]
PF00198;PF00364;PF02817;
2.40.50.100;3.30.559.10;4.10.320.10;
2-oxoacid dehydrogenase family
null
null
CATALYTIC ACTIVITY: Reaction=N(6)-[(R)-dihydrolipoyl]-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = CoA + N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2]; Xref=Rhea:RHEA:15213, Rhea:RHEA-COMP:10581, Rhea:RHEA-COMP:10582, ChEBI:CHEBI:57287,...
null
PATHWAY: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
null
null
FUNCTION: E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2). {ECO:0000305|PubMed:17367808}.
Escherichia coli (strain K12)
P0AFG8
ODP1_ECOLI
MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEARKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLKDTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVD...
1.2.4.1
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; COFACTOR: Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
pyruvate catabolic process [GO:0042867]
cytosol [GO:0005829]; cytosolic pyruvate dehydrogenase complex [GO:0045250]; membrane [GO:0016020]; pyruvate dehydrogenase complex [GO:0045254]
identical protein binding [GO:0042802]; magnesium ion binding [GO:0000287]; molecular adaptor activity [GO:0060090]; protein homodimerization activity [GO:0042803]; pyruvate dehydrogenase (acetyl-transferring) activity [GO:0004739]; pyruvate dehydrogenase activity [GO:0004738]; small molecule binding [GO:0036094]; thia...
PF17831;PF00456;
3.40.50.920;3.40.50.970;
null
null
null
CATALYTIC ACTIVITY: Reaction=H(+) + N(6)-[(R)-lipoyl]-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + pyruvate = CO2 + N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase]; Xref=Rhea:RHEA:19189, Rhea:RHEA-COMP:10480, Rhea:RHEA-COMP:10481, ChEBI:CHEBI:15361, ChEBI:CHEBI:1...
null
null
null
null
FUNCTION: Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).
Escherichia coli (strain K12)
P0AFI0
OXC_ECOLI
MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIILGKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVG...
4.1.1.8
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:20553497}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000269|PubMed:20553497}; COFACTOR: Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000269|PubMed:20553497}; Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000269|Pu...
cellular response to acidic pH [GO:0071468]; fatty acid alpha-oxidation [GO:0001561]; oxalate catabolic process [GO:0033611]
peroxisome [GO:0005777]
ADP binding [GO:0043531]; identical protein binding [GO:0042802]; magnesium ion binding [GO:0000287]; oxalyl-CoA decarboxylase activity [GO:0008949]; thiamine pyrophosphate binding [GO:0030976]
PF02775;PF00205;PF02776;
3.40.50.970;3.40.50.1220;
TPP enzyme family
null
null
CATALYTIC ACTIVITY: Reaction=H(+) + oxalyl-CoA = CO2 + formyl-CoA; Xref=Rhea:RHEA:19333, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57376, ChEBI:CHEBI:57388; EC=4.1.1.8; Evidence={ECO:0000269|PubMed:20553497};
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.17 uM for oxalyl-CoA (with 300 uM ADP at pH 6.5 and 30 degrees Celsius) {ECO:0000269|PubMed:20553497}; KM=4.8 uM for oxalyl-CoA (at pH 6.5 and 30 degrees Celsius) {ECO:0000269|PubMed:20553497}; Note=kcat is 69.7 sec(-1) for decarboxylase activity with oxalyl-CoA ...
PATHWAY: Metabolic intermediate degradation; oxalate degradation; CO(2) and formate from oxalate: step 2/2.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is between 5.5 and 7. {ECO:0000269|PubMed:20553497};
null
FUNCTION: Involved in the catabolism of oxalate and in the adapatation to low pH via the induction of the oxalate-dependent acid tolerance response (ATR). Catalyzes the decarboxylation of oxalyl-CoA to yield carbon dioxide and formyl-CoA. {ECO:0000269|PubMed:20553497}.
Escherichia coli (strain K12)
P0AFI2
PARC_ECOLI
MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRRIVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDGQGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPARLPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTEAEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRNKKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTD...
5.6.2.2
null
chromosome segregation [GO:0007059]; DNA topological change [GO:0006265]; plasmid partitioning [GO:0030541]; sister chromatid cohesion [GO:0007062]
chromosome [GO:0005694]; cytoplasm [GO:0005737]; cytosol [GO:0005829]; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex [GO:0009330]; extrinsic component of plasma membrane [GO:0019897]
ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity [GO:0003918]
PF03989;PF00521;
3.30.1360.40;2.120.10.90;3.90.199.10;1.10.268.10;
Type II topoisomerase GyrA/ParC subunit family, ParC type 1 subfamily
null
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CATALYTIC ACTIVITY: Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-Rule:MF_00936, ECO:0000269|PubMed:12269820, ECO:0000269|PubMed:16023670, ECO:0000269|PubMed:21300644};
null
null
null
null
FUNCTION: Topoisomerase IV is essential for chromosome segregation; it is the principal protein responsible for decatenating newly replicated chromosomes (PubMed:9334322). It relaxes supercoiled DNA (PubMed:12269820, PubMed:16023670, PubMed:21300644). MukB stimulates the relaxation activity of topoisomerase IV and also...
Escherichia coli (strain K12)
P0AFI5
PBP7_ECOLI
MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND
3.4.21.-
null
cell wall organization [GO:0071555]; FtsZ-dependent cytokinesis [GO:0043093]; peptidoglycan biosynthetic process [GO:0009252]; peptidoglycan metabolic process [GO:0000270]; proteolysis [GO:0006508]; regulation of cell shape [GO:0008360]; response to xenobiotic stimulus [GO:0009410]
periplasmic space [GO:0042597]; TRAPP complex [GO:0030008]
endopeptidase activity [GO:0004175]; serine-type D-Ala-D-Ala carboxypeptidase activity [GO:0009002]
PF00768;
3.40.710.10;
Peptidase S11 family
PTM: Pbp8 is a proteolytic product of Pbp7.
SUBCELLULAR LOCATION: Periplasm.
null
null
null
null
null
FUNCTION: Cell wall formation. May play a specialized role in remodeling the cell wall. Specifically hydrolyzes the DD-diaminopimelate-alanine bonds in high-molecular-mass murein sacculi.
Escherichia coli (strain K12)
P0AFI7
PDXH_ECOLI
MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACEAKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLLFPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKFLELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
1.4.3.5
COFACTOR: Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:10903950, ECO:0000269|PubMed:11453690, ECO:0000269|PubMed:11786019, ECO:0000269|PubMed:15858270, ECO:0000269|PubMed:7860596, ECO:0000269|PubMed:9693059}; Note=Binds 1 FMN per subunit. {ECO:0000269|PubMed:10903950, ECO:0000269|PubMed:11453690, ECO:...
'de novo' pyridoxal 5'-phosphate biosynthetic process [GO:0036001]; pyridoxal phosphate biosynthetic process [GO:0042823]; pyridoxine biosynthetic process [GO:0008615]
cytosol [GO:0005829]; protein-containing complex [GO:0032991]
FMN binding [GO:0010181]; oxidoreductase activity [GO:0016491]; phosphate ion binding [GO:0042301]; protein homodimerization activity [GO:0042803]; pyridoxal phosphate binding [GO:0030170]; pyridoxamine phosphate oxidase activity [GO:0004733]; riboflavin binding [GO:1902444]
PF10590;PF01243;
null
Pyridoxamine 5'-phosphate oxidase family
null
null
CATALYTIC ACTIVITY: Reaction=H2O + O2 + pyridoxamine 5'-phosphate = H2O2 + NH4(+) + pyridoxal 5'-phosphate; Xref=Rhea:RHEA:15817, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:28938, ChEBI:CHEBI:58451, ChEBI:CHEBI:597326; EC=1.4.3.5; Evidence={ECO:0000269|PubMed:11786019, ECO:0000269|PubMed:78605...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.3 uM for pyridoxamine 5'-phosphate {ECO:0000269|PubMed:11786019}; KM=2 uM for pyridoxine 5'-phosphate (at pH 7.6 and 37 degrees Celsius) {ECO:0000269|PubMed:7860596, ECO:0000269|PubMed:9693059}; KM=105 uM for pyridoxamine 5'-phosphate (at pH 7.6 and 37 degrees Ce...
PATHWAY: Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.; PATHWAY: Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
null
null
FUNCTION: Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). {ECO:0000269|PubMed:11786019, ECO:0000269|PubMed:7860596, ECO:0000269|PubMed:9693059}.
Escherichia coli (strain K12)
P0AFJ7
PITA_ECOLI
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLIVSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQHPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKG...
null
null
magnesium ion homeostasis [GO:0010960]; phosphate ion transmembrane transport [GO:0035435]; tellurite transport [GO:0015710]
plasma membrane [GO:0005886]
inorganic phosphate transmembrane transporter activity [GO:0005315]; solute:proton symporter activity [GO:0015295]; tellurite transmembrane transporter activity [GO:0015654]; zinc ion transmembrane transporter activity [GO:0005385]
PF01384;
null
Inorganic phosphate transporter (PiT) (TC 2.A.20) family, Pit subfamily
null
SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:11489853, ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000255}.
CATALYTIC ACTIVITY: Reaction=H(+)(in) + phosphate(in) = H(+)(out) + phosphate(out); Xref=Rhea:RHEA:29939, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474; Evidence={ECO:0000269|PubMed:328484, ECO:0000269|PubMed:8110778, ECO:0000305|PubMed:6998957}; CATALYTIC ACTIVITY: Reaction=arsenate(in) + H(+)(in) = arsenate(out) + H(+)(out); ...
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.9 uM for phosphate {ECO:0000269|PubMed:11489853}; Vmax=58 nmol/min/mg enzyme (at pH 6.6 and 1.8 mM Mg(2+)) {ECO:0000269|PubMed:11489853}; Vmax=39 nmol/min/mg enzyme (at pH 7.0 and 10.0 mM Mg(2+)) {ECO:0000269|PubMed:11489853};
null
null
null
FUNCTION: Low-affinity inorganic phosphate transporter (PubMed:11489853, PubMed:328484, PubMed:6998957, PubMed:8110778). Mediates proton-driven uptake of soluble neutral metal phosphate (MeHP04) complexes (PubMed:8110778). It can use Mg(2+), Ca(2+), Co(2+) and Mn(2+) (PubMed:8110778). Activity impacts bacterial growth ...
Escherichia coli (strain K12)
P0AFL3
PPIA_ECOLI
MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVADKISQVPTHDVGPYQNVPSKPVVILSAKVLP
5.2.1.8
null
protein folding [GO:0006457]
outer membrane-bounded periplasmic space [GO:0030288]; periplasmic space [GO:0042597]
peptidyl-prolyl cis-trans isomerase activity [GO:0003755]
PF00160;
2.40.100.10;
Cyclophilin-type PPIase family
null
SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:2190212}.
CATALYTIC ACTIVITY: Reaction=[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0); Xref=Rhea:RHEA:16237, Rhea:RHEA-COMP:10747, Rhea:RHEA-COMP:10748, ChEBI:CHEBI:83833, ChEBI:CHEBI:83834; EC=5.2.1.8; Evidence={ECO:0000269|PubMed:2190212}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16238;...
null
null
null
null
FUNCTION: PPIases accelerate the folding of proteins (Probable). It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed:2190212). {ECO:0000269|PubMed:2190212, ECO:0000305}.
Escherichia coli (strain K12)
P0AFL6
PPX_ECOLI
MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFLKRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPILVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASGTIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLAILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTAS...
3.6.1.11
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:8380170};
polyphosphate catabolic process [GO:0006798]
plasma membrane [GO:0005886]
exopolyphosphatase activity [GO:0004309]; guanosine tetraphosphate binding [GO:0097216]; identical protein binding [GO:0042802]; protein homodimerization activity [GO:0042803]
PF02541;PF21447;
3.30.420.40;3.30.70.2260;3.30.420.150;1.10.3210.10;
GppA/Ppx family
null
SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:8380170}; Peripheral membrane protein {ECO:0000305|PubMed:8380170}.
CATALYTIC ACTIVITY: Reaction=[phosphate](n) + H2O = [phosphate](n-1) + H(+) + phosphate; Xref=Rhea:RHEA:21528, Rhea:RHEA-COMP:9859, Rhea:RHEA-COMP:14279, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16838, ChEBI:CHEBI:43474; EC=3.6.1.11; Evidence={ECO:0000269|PubMed:16905100, ECO:0000269|PubMed:8380170, ECO:000026...
null
null
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8. {ECO:0000269|PubMed:8380170};
null
FUNCTION: Degradation of inorganic polyphosphates (polyP). Releases orthophosphate processively from the ends of the polyP chain. Has a strong preference for long-chain polyphosphates and has only weak affinity for smaller size polyP of about 15 residues. {ECO:0000269|PubMed:16905100, ECO:0000269|PubMed:8380170, ECO:00...
Escherichia coli (strain K12)
P0AFM2
PROX_ECOLI
MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQTLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKKFYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPGWGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNELKPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLFAIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDG...
null
null
amino acid import across plasma membrane [GO:0089718]; cellular response to osmotic stress [GO:0071470]; glycine betaine transport [GO:0031460]; glycine import across plasma membrane [GO:1903804]; hyperosmotic response [GO:0006972]
membrane [GO:0016020]; outer membrane-bounded periplasmic space [GO:0030288]; ProVWX complex [GO:1990222]
quaternary ammonium group binding [GO:0050997]; transmembrane transporter activity [GO:0022857]
PF04069;
3.40.190.10;
null
null
SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:3305496, ECO:0000269|PubMed:7898450}.
null
null
null
null
null
FUNCTION: Part of the ProU ABC transporter complex involved in glycine betaine and proline betaine uptake. Binds glycine betaine and proline betaine with high affinity. {ECO:0000269|PubMed:14612446, ECO:0000269|PubMed:23249124, ECO:0000269|PubMed:3305496, ECO:0000269|PubMed:7898450}.
Escherichia coli (strain K12)
P0AFM6
PSPA_ECOLI
MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIKSLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGKLDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMKQDNQ
null
null
phage shock [GO:0009271]; regulation of cellular response to stress [GO:0080135]; regulation of DNA-templated transcription [GO:0006355]; response to heat [GO:0009408]
cell pole [GO:0060187]; cytosol [GO:0005829]; plasma membrane [GO:0005886]; transcription regulator complex [GO:0005667]
identical protein binding [GO:0042802]; phospholipid binding [GO:0005543]
PF04012;
null
PspA/Vipp/IM30 family
null
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19555453}. Cell inner membrane {ECO:0000269|PubMed:19555453}; Peripheral membrane protein {ECO:0000269|PubMed:19555453}; Cytoplasmic side {ECO:0000269|PubMed:19555453}. Note=Localizes at both cell poles and along the length of the cell.
null
null
null
null
null
FUNCTION: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation of the psp-specific transcriptional activator PspF. Is...
Escherichia coli (strain K12)
P0AFP6
GCH1L_ECOLI
MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTASQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDAHPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDTGPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHATERGGIRALSEWLNENTDLDVTFIDIPNPA
null
null
DNA repair [GO:0006281]; protein hexamerization [GO:0034214]; response to ionizing radiation [GO:0010212]
cell pole [GO:0060187]; cytoplasm [GO:0005737]; cytosol [GO:0005829]
identical protein binding [GO:0042802]; metal ion binding [GO:0046872]
PF01784;
3.40.1390.30;
GTP cyclohydrolase I type 2/NIF3 family
null
null
null
null
null
null
null
FUNCTION: Provides significant protection from radiation damage and may be involved in the degradation of radiation-damaged nucleotides. {ECO:0000269|PubMed:25049088}.
Escherichia coli (strain K12)