ids
stringlengths
6
10
seqs
stringlengths
11
1.02k
texts
stringlengths
108
11.1k
B1A0U4
MALSGLGSGPEGNPNNHQGKAIPTLNPSHGQGPSFLFSWVSFLVPFWSWYPFSPLPLKGIPGENSSSPNGLKNFHGIGLNGTERGMGHFHPISGNRLGLRLTLVAGWFLEPFPTFLAVLIRTQGDSTPSRSFVAFFVGTLGLGQEGITGSFNRKIAGPATIDPQDWAMEEGVLTNFLWPPFTTFFRTTKGFPVQWHGEGSFVVPGIFSLPQPLPWWLLLPDTPTGAGKPPESRQHQPSHSMNGAIVDIPGGLTQTPSSPDRSSSTNSIADEEKKLDYKPTSPTSDTEKGESLPLTASQSEIIASPTFSEMIRVIFSGPTLVLGACYFCTFGAELSINSVLGTFYQRQLGLGLQNAGNLAAIFGLLNIVMRPLGGMASDLLYRKTGSVWSKKALLHTYCVMTGVFCIAIGLARSRSQATLVGLVSGGLAFFLEGANGLTYSHVHPYANGVVSGFTGACGNLGGIVFAIVFRYNSLDYSKVFWIIGAIIIGLQVATCWIKPVPKSVI
Function: Major facilitator-type transporter; part of the gene cluster that mediates the biosynthesis of elsinochromes, pigments consisting of at least four interconvertible tautomers (A, B, C and D) that have a core phenolic quinone to which various side chains are attached and which play an important role in fungal pathogenesis . Once elsinochrome is synthesized, it must be exported outside the fungal cells, which is probably accomplished by the ECT1 transporter, to avoid toxicity . Location Topology: Multi-pass membrane protein Sequence Mass (Da): 54058 Sequence Length: 505 Subcellular Location: Cell membrane
Q8SQW6
MASHEIQKVWADGCFDMFHYGHANALRQSKALGDYLIAGVHSSLSINQEKGLPVMEDEERYEVVEGCRYVDEVVRDAPFVTQTSMIKEYGVSIVAHGNDIVLDSSGQDSYCQVRRMGIFREVERTFGISTTEIVGRMMLKNRGSWLDGENGESSKDSGYHDRLLSLFMSSMGREKRGKVVFMDGNFDLFHAGHVASLRIARGMGDYLIVGIHDDETTKEYTRSYPVLSTKERMLTLMACRYVDEIVVSPYLVGSEFIKRHGIDVVAPSFDSKDLSRYDGIKDVVEHSYAENRFNYLSAEHIVNRIISNYQDYANRQKKRTGK
Catalytic Activity: CTP + H(+) + phosphoethanolamine = CDP-ethanolamine + diphosphate Sequence Mass (Da): 36594 Sequence Length: 322 Pathway: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3. EC: 2.7.7.14
Q9UTI6
MASSSNIKHRLWLDGCMDFFHYGHSNAILQAKQLGETLVIGIHSDEEITLNKGPPVMTLEERCLSANTCKWVDEVVPSAPYVFDLEWMRRYGCQYVVHGDDISTDANGDDCYRFAKAADQYLEVKRTEGVSTTELLDRLLSSVPLEIYSTPVSVLSSQIDLLRRFATDSDGLTPFTDVFIYNTEKPETLISGTTLLRLNPEKNIIYIDGDWDLFTEKHISALELCTRMFPGIPIMAGIFADEKCFEKPMLNLLERILNLLQCKYISSILVGPPPASLFASSKYIKLCFDEQISKVYYPIFSTDVSIPALDISLSNTPNNSFYKFDKLGSDLIKQRVMLRRQHYEERQRRKMGKNATEQTTIKTYA
Catalytic Activity: CTP + H(+) + phosphoethanolamine = CDP-ethanolamine + diphosphate Sequence Mass (Da): 41557 Sequence Length: 365 Pathway: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3. EC: 2.7.7.14
P33412
MTVNLDPDKVWIDGCFDFTHHGHAGAILQARRTVSKENGKLFCGVHTDEDIQHNKGTPVMNSSERYEHTRSNRWCSEVVEAAPYVTDPNWMDKYQCQYVVHGDDITIDANGEDCYKLVKEMGRFKVVKRTYGVSTTEIIHRILTKKSLPPTHPDYYPTTQELSFYSVAQDAVSKHCYVFQRDLDNVLVNGGYKFDAEDCVYVDGDFDLFHMGDIDQLRKLKMDLHPDKKLIVGITTSDYSSTIMTMKERVLSVLSCKYVDAVIIDADATSMSQYNCEKYHIGTAVLTAAGKFSEYLTKELIVKRVESQREVYIARNQKKGMSI
Function: Ethanolamine-phosphate cytidylyltransferase which catalyzes the second step of phosphatidylethanolamine biosynthesis. Involved in the maintenance of plasma membrane and required for proper sporulation. Catalytic Activity: CTP + H(+) + phosphoethanolamine = CDP-ethanolamine + diphosphate Sequence Mass (Da): 36863 Sequence Length: 323 Pathway: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3. Subcellular Location: Cytoplasm EC: 2.7.7.14
Q9H8V3
MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALKTIKIMEVPVIKIKESCPGKSDEKLIKSVINMDIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSMLSLNTPNSNRKRRRLKETLAQLSRETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQSARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHGQTRPPASLKHIHLMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSDELPKENWLKMLCRHVANTICKADAENLIYTADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRRALMTSHGSVEGRSPSSNDKHVMSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTTHLI
Function: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. PTM: Phosphorylated by PLK1 in vitro. Hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at Thr-373 occurs during the G2/M phase, relieves its auto-inhibition status and stimulates its GEF activity. Phosphorylation at Thr-444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange activation. Dephosphorylated at the time of cytokinesis. Phosphorylation at Thr-359 is required for its transformation activity in cancer cells. Sequence Mass (Da): 103505 Sequence Length: 914 Domain: The BRCT domains 1 and 2 are required for intramolecular interaction, but not for intermolecular oligomerization . The BRCT domains negatively inhibit its GEF activity in interphase cells . The same BRCT domains may act as a positive regulatory motif for the completion of cytokinesis after the breakdown of nuclear membrane during mitosis . Subcellular Location: Nucleus
Q07139
MADDSVLPSPSEITSLADSSVFDSKVAEMSKENLCLASTSNVDEEMPQVEARVIMVQDAGKQEELLKALKTIKIMEVPVIKIKESCPGKSEEKLIKSIINMEMKVPCVKMDSMEEFESLDSPEFENIFVVTDFQNSVFNDLYKADCRIVGPPVILNCAQRGEPLPFSCRPLYCTSMLNLVLCFTGFRKKEELVKLVTLVHHMGGVIRKECNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQCFCAAVDDFRNEFKVPPFQDCILSFLGFSDEEKHSMEEMTEMQGGSYLPVGDERCTHLIVEENTVKDLPFEPSKKLFVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSLLSLSTPNSNRKRRRLKETLAQLSRETDLSPFPPRKRPSAEHSLSIGSLLDISNTPESSIHYGETPKSCAKSSRSSTPVPPKQSARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHMKIKDDLEDLIANWDESRSIGDIFLKYAKDLVKTYPPFVNFFEMSKEMIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETVSLGEHPCDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHDRTRPPASLKHIHLMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSEELPKESWLKMLCRHVANTICKADAENLMYVADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRMALSSSHSSEGRSPPSSGKLAVSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTTHLI
Function: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. PTM: Phosphorylated by PLK1 in vitro. Hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at Thr-373 occurs during the G2/M phase, relieves its auto-inhibition status and stimulates its GEF activity. Phosphorylation at Thr-444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange activation. Dephosphorylated at the time of cytokinesis. Phosphorylation at Thr-359 is required for its transformation activity in cancer cells (By similarity). Sequence Mass (Da): 103131 Sequence Length: 913 Domain: The BRCT domain 1 and 2 are required for the intramolecular interaction, but not for the intermolecular oligomerization. The BRCT domains negatively inhibit its GEF activity in interphase cells. The same BRCT domains may act as a positive regulatory motif for the completion of cytokinesis after the breakdown of nuclear membrane during mitosis (By similarity). Subcellular Location: Nucleus
Q7W980
MRKDETSNTSPDISVAQPASALRYHLRPPRRNDGAAIHQLVSECPPLDLNSLYAYLLLCEHHAHTCVVAESPGGRIDGFVSAYLLPTRPDVLFVWQVAVHSRARGHRLGRAMLGHILERQECRHVRHLETTVGPDNQASRRTFAGLAGERGAHVSEQPFFDRQAFGGADHDDEMLLRIGPFTHPPH
Function: Catalyzes the acetylation of L-2,4-diaminobutyrate (DABA) to gamma-N-acetyl-alpha,gamma-diaminobutyric acid (ADABA) with acetyl coenzyme A. Catalytic Activity: acetyl-CoA + L-2,4-diaminobutanoate = (2S)-4-acetamido-2-aminobutanoate + CoA + H(+) Sequence Mass (Da): 20729 Sequence Length: 186 Pathway: Amine and polyamine biosynthesis; ectoine biosynthesis; L-ectoine from L-aspartate 4-semialdehyde: step 2/3. EC: 2.3.1.178
O52249
MNATTEPFTPSADLAKPSVADAVVGHEASPLFIRKPSPDDGWGIYELVKSCPPLDVNSAYAYLLLATQFRDSCAVATNEEGEIVGFVSGYVKSNAPDTYFLWQVAVGEKARGTGLARRLVEAVMTRPEMAEVHHLETTITPDNQASWGLFRRLADRWQAPLNSREYFSTDQLGGEHDPENLVRIGPFQTDQI
Function: Catalyzes the acetylation of L-2,4-diaminobutyrate (DABA) to gamma-N-acetyl-alpha,gamma-diaminobutyric acid (ADABA) with acetyl coenzyme A. Does not acetylate amino acids like GABA, L-ornithine, L-lysine and L-aspartate. Catalytic Activity: acetyl-CoA + L-2,4-diaminobutanoate = (2S)-4-acetamido-2-aminobutanoate + CoA + H(+) Sequence Mass (Da): 21170 Sequence Length: 192 Pathway: Amine and polyamine biosynthesis; ectoine biosynthesis; L-ectoine from L-aspartate 4-semialdehyde: step 2/3. EC: 2.3.1.178
P68104
MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK
Function: Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis . Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome . The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation . Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 . PTM: ISGylated. Catalytic Activity: GTP + H2O = GDP + H(+) + phosphate Sequence Mass (Da): 50141 Sequence Length: 462 Subcellular Location: Cytoplasm EC: 3.6.5.-
P86934
MGKEKVHMNLVVVGHVDAGKSTATGHLIYKCGGIDKRTIEKFEKEAADIGKASFKYAWVLDKLKAERERGITIDIALWKFESPKSVFTIIDAPGHRDFIKNMITGTSQADAAILIIASAQGEFEAGISKDGQTREHALLAFTLGVKQMVVCCNKMDDKTVNYGQERYDEIVKEVSAYIKKVGYNVEKVRFVPISGWQGDNMIEKSEKMPWYKGPTLLEALDMLEPPVRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVMKPGDVVTFAPANVTTEVKSIEMHHEQLAEATPGDNVGFNVKNVSVKDIRRGNVCGNTKNDPPKEAADFTAQVIILNHPGQIGNGYAPVLDCHTSHIACKFAEIESKIDRRSGKELEKAPKSIKSGDAAIVRMVPQKPMCVEVFNDYAPLGRFAVRDMRQTVAVGIIKAVTKKDGSGGKVTKAAVKASKK
Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. PTM: Phosphatidylethanolamine (PE) is a direct precursor of the ethanolamine-phosphoglycerol (EPG) moiety. Sequence Mass (Da): 49106 Sequence Length: 449 Subcellular Location: Cytoplasm
Q05639
MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK
Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. PTM: Trimethylated at Lys-165 by EEF1AKMT3 . Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs . Trimethylated at the N-terminus by METTL13 . Mono- and dimethylated at Lys-55 by METTL13; dimethylated form is predominant . Sequence Mass (Da): 50470 Sequence Length: 463 Subcellular Location: Nucleus
P60608
MNSPCDRLQQFIQVLLEESWSFPSFANTLHWPENLLSYIDELVWQGSLQNFHQHEVRFDKPPLRLPLTGFSSLTENWSSRQAVSSRLVATAASPPAGCQAPIAFLGLKFSSLGPARKNPALCFLYDQSNSKCNTSWVKENVGCPWHWCNIHEALIRTEKGSDPMFYVNTSTGGRDGFNGFNLQISDPWDPRWASGVDGGLYEHKTFMYPVAKIRIARTLKTTVTGLSDLASSIQSAEKELTSQLQPAADQAKSSRFSWLTLISEGAQLLQSTGVQNLSHCFLCAALRRPPLVAVPLPTPFNYTINSSTPIPPVPKGQVPLFSDPIRHKFPFCYSTPNASWCNQTRMLTSTPAPPRGYFWCNSTLTKVLNSTGNHTLCLPISLIPGLTLYSQDELSHLLAWTEPRPQNKSKWAIFLPLVLGISLASSLVASGLGKGALTHSIQTSQDLSTHLQLAIEASAESLDSLQRQITTVAQVAAQNRQALDLLMAEKGRTCLFLQEECCYYLNESGVVENSLQTLKKKKSSKRS
Function: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties. PTM: The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane domain (By similarity). Sequence Mass (Da): 58319 Sequence Length: 527 Domain: The CKS-17 immunosuppressive domain is present in many retroviral envelope proteins. As a synthetic peptide, it inhibits immune function in vitro and in vivo (By similarity). Subcellular Location: Virion
A7RR04
MGIITLLANRRGLHAILSNAQRIVGCRFVSSSIIPDASRLRNIGISAHIDSGKTTLTERLLFYTGRISHMHEVKGKDNVGATMDSMELERQRGITIQSAATYVNWKDHNINIIDTPGHVDFTVEVERALRVLDGAVLVLCAVGGVQSQTLTVNRQMKRYNVPCIAFINKLDRMGANHNRVLSQLRAKLNHNAALLQLPVGLEGNNTGVVDIIRWKAYYFDGDNGEIVREDVIPEDMVDECRKRRQELIEVVADVDPELGDLFLEEVKPSESQIIAAIRRATIERTFTPVFVGSALKNKGVQPLLDGVLDYLPNPTEVKNYALDAESLDTKVLMDSRRSGEAPFVGLAFKLEAGRYGQLTYLRVYQGALKRGGFIVNTRTGKRVKVPRIVRMHSDIMEDIQEGYAGDICALFGIECASGDTFTAEGAPLVSMESIFVPEPVISLAVEPKNKNDLDQFSKAINRFTREDPTFRVRFDDESKETIISGMGELHLDVYTERMRLEYNCPVICGKPKVAFRETIGKEASLDFRPKSRVAAAGQYGKVVGKIEPMPPESITKNEFVDATVGMNIPKNFIPAIEKGFYEACERGFITGHKVAGVRFVLEDGAAHAVDSSEMAFRMATIGAFREAFNKAAPMILEPIMSVEVNAPQEFQGTVIAGVNRRHGQVTGTDANEGYFTLFAEVPLNDMFGYATELRSQTQGKGEFTMEYCRYLPALAQVQAELMDRFNVEKLKKAKR
Function: Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Sequence Mass (Da): 81675 Sequence Length: 735 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Mitochondrion
Q07803
MRLLRITAGLGRGPLPRVPAILGWQGKQANWKTYRWCSSGSIPNEKIRNIGISAHIDSGKTTLTERVLYYTGRIATMHEVKGKDGVGAVMDSMELERQRGITIQSAATYTMWRDVNINIIDTPGHVDFTIEVERALRVLDGAVLVLCAVGGVQCQTMTVSRQMKRYNVPFLTFINKLDRMGSNPARALQQMRSKLNHNAAFVQIPIGLEGDFKGIIDLIEERAIYFDGDFGQIVRYDEIPADLRAAAADHRQELIECVANSDEQLGELFLEEKIPSVSDLKLAIRRATLSRSFTPVFLGSALKNKGVQPLLDAVLEFLPNPSEVQNYALLNQNDSKEKNKILMNPKRDDSHPFVGLAFKLEAGRFGQLTYVRNYQGELKKGSTIYNTRTGKKVRVQRLVRMHADMMEDVEEVYAGDICALFGIDCASGDTFTNKDNSDLSMESIHVPDPVISVAMKPSNKNDLEKFSKGIARFTREDPTFKVHFDTESKETIVSGMGELHLEIYAQRMEREYGCPCITGKPKVAFRETVTAPVPFDFTHKKQSGGAGQYGKVIGVLEPLAPEDYTKLEFSDETFGANVPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGEGALKQALASATLCIIEPIMSVEVIAPNEFQGAVFAGINRRHGVITGQDGIEDYFTLYADVPLNNMFGYSTELRSCTEGKGEYTMEYNRYQPCSPSTQEELVNKYLEATGQLPVKKGKAKN
Function: Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Sequence Mass (Da): 83457 Sequence Length: 751 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Mitochondrion
Q9USZ1
MLKLSFRSLTSRLPRLSTLVVRGYASVANTGIEASNTSENNLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGAKMDFMELEREKGITIQSAATHCTWERTVDQIEANEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDRMGADPWKVIQQINTKLKIPAAAVQIPIGQEDKLEGVVDLIQMRAIYNRGSKGEKIEISQQVPENLIELAKEKRSALIEKLADLDEEIADIYVMEEDPTPEQLMGAIRRTTLARKFTPVLMGSALSNVGVQSVLDAVCDYLPNPSEVENIALNAADSEKPVSLVPSSEKPLVALAFKLEEGRFGQLTYLRIYQGTLKRGNYIYNVNSTKKIKVSRLVRMHSNDMEEIEKVEAGGICALFGIECASGDTFTDGSVSYTMTSMFVPEPVISLSLKPKSKDTTSFSKALNRFQREDPTFRVQLDNESKETIISGMGELHLEVYVERMRREYKVDCETGKPRVAFRETLSKKVPFSYLHKKQSGGAGQYAKVEGYIEYMDGVEDESGNVVDCEFINKVTGGTVPTQYIPACEKAFYEALKKGFLIGHPIKNCRFVLEDGAYHPVDSSELAFRLATISAFRTAFLQANPMVLEPIMNVSITAPVEHQGGVIGNLDKRKATIVDSDTDEDEFTLQAEVPLNSMFSYSSDIRALTKGKGEFSMEFLKYLPAPKYVQKELVDAYNKQQQK
Function: Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Sequence Mass (Da): 86325 Sequence Length: 770 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Mitochondrion
A7EVV9
MSMHRVARAVASSEACAGALRGSVSRTRFLCLNAPVQRFGARNALLGAGLGAKRHFFQSPIIRSGVAQAVLEKAAADPSALTQEAIVDNLNAAERDRLRRVRNIGIAAHIDSGKTTATERVLFYTGRINAIHEVRGKDAVGAKMDSMELEREKGITIQSAATFCDWMKVENGKEEKYHINLIDTPGHIDFTIEVERALRVLDGAVMILCAVSGVQSQTITVDRQMRRYNVPRISFINKMDRMGANPFKAVEQINQKLRIPAAALQVPIGSEDSFNGVVDLIRMKAIYNDGPKGEIIRETDEIPEELKQLCKEKRALLIETLADVDDEIAEIFLDEKTPSIEQMKAAIRRATINLKFTPVLMGSALADKSVQPMLDAVCDYLPNPAEVENLALDKRRAEAPVKLVSYNELPFVGLAFKLEESNYGQLTYIRVYQGSLRKGMNVFNARTDKRVKIPRIVRMHSNEMEEVPEIGAGEICAVFGVDCASGDTFTDGGLPYSMSSMFVPDPVISLSIKPKTTKDGSNFSKAMNRFQREDPTFRVHVDAESQETIISGMGELHLDIYVERMRREYKVEVETGKPQVAYRETITEHVTFDHTLKKQTGGAGDYARVVGFLEPIEAGPNGYAPSTFKEEVTGGSISDKFLFACEKGFLASCEKGPLLGHPVLGTHMVVNDGATHMTDSSEMAFKNATQQAFRKAFKEGKPQVLEPLMKTTITAPNEFQGNIVGLLNKRNAIISDTEIGPEDFTLIADCSLNAMFGFSSQLRAATQGKGEFGMEFSHYAPAPGQLQKELISNYEKAQADRHKK
Function: Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Sequence Mass (Da): 88744 Sequence Length: 804 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Mitochondrion
B8MJJ5
MVRPAQVRALSGLARSATFVRLLPSQSQNALRCASLPVSRLGALPLRATTQITSAPLRQWHQIRNSSATATASLAEQAAADPEGLSQAEIISNIDAEEWKRISKVRNIGIAAHIDSGKTTATERVLFYTGRINAIHEVRGRDSVGAKMDSMDLEREKGITIQSAATFCDWVKKENGKEEKYHFNLIDTPGHIDFTIEVERALRVLDGAVMILCAVSGVQSQTITVDRQMKRYNVPRISFVNKMDRMGANPFKAIDQINNKLKLPAAAVQVPIGAEDEFQGVVDLIRMKAIYNEGPRGETIVEKDEIPEHIKPLAEERRRILIETLADVDDEIAEIFLDEREPTIEQIKAAIRRATIALKFTPVFMGSALADKSIQPMLDGVCDYLPNPSEVTNLALDQKRKEAQVKLLPYGSQPFVGLAFKLEESNFGQLTYIRVYQGTLRKGANVFNARNDKKVKVPRIVRMHSNEMEEVQEIGAGEICAVFGVDCASGDTFTDGQLAYTMSSMFVPEPVISLSIKPKNNKDSANFSKAMARFQREDPTFRVSYDTESEQTIISGMGELHLDIYVERMRREYKVDCETGQPQVAYRETIGRRVEFDHLLKKQSGGPGDYARVVGWMEPSESLEENKFEEQIVGGAISEKFLFACEKGFNLACEKGPLIGHKVLGTKMVINDGATHMTDSSEMSFKNATQQAFRKAFMESQPHVLEPLMKTVVTAPIEFQGDVIGLLNKRNATINDSEIGVDEFTVYADCSLNGMFGFSSHLRAATQGKGEYTMEFSHYEKAPGQLQKELVQKYLKAQADRHKK
Function: Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Sequence Mass (Da): 89606 Sequence Length: 804 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Mitochondrion
Q4P257
MVAIPRVAAARSLARQLARQSLRTTSFASAPVRIAIASTPLARSPSSFRSLSSSTRRSAAAAAAAAAAKATPAHDDSHTPMAVLTEADLGRLVRQRNVGISAHIDSGKTTLTERVLFYTGRIKDIHEVRGRDAVGAKMDHMELEREKGITIQSAATYCSWKATPPTEKASVSGDAANVESKELMEKKQDFHINIIDTPGHVDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRYSVPRISFINKMDRAGANPWRVIGQIRNKLKMPAAAVQIPIGAEDDFNGVIDLIRWKAVYNEGHKGIDIRETDEIPAEYLELAKQKRAELIEQLAEVDDEMTEMFIEEREPTIEELAAAIRRTTIRCQFSPVFLGSAIKNKGVQAMLDGVCSYLPNPAEVPATAMDMSSAATKKAAEEAAQAAGEDQEAAAEARKNAAPPVLPLSPASEAPLVGLAFKLEEGKYGQLTYMRVYQGTLKRGNLIFNARTGKKVKVPRLVRMHSNDMEDVDEIGAGEICAMFGVECSSGDTFTDGTTQLSMTSMFVPEPVISLAITPEGKESQNFSRALNRFQKEDPTFRVHVDKESNETIISGMGELHLEIYVERMRREYNVPCTTGKPRVAFRETIEKKATFAYTHKKQTGGAGQFGRVMGYIEPMEVDPETGVDTAFDNRVVGGSIPNGYISACEKGFYDALEKGALSGHAVTGVRFVLEDGAAHSVDSSELAFRLATAGAFREAYQKANPVILEPKMTVEVVAPIEFQGAVIGALNQRKGTISDTEVREDEFTLTAEVSLNDMFGYSSQLRGLTQGKGEFSMEYKCHTPVMMNIQKEMHEAYRKKQTEKK
Function: Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Sequence Mass (Da): 92219 Sequence Length: 842 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Mitochondrion
P38732
MITQTELDNCLQWAQNNGAFIDPKISFRITEDAGVSAFVNEKFSPKPDQALIRVPETLLITSQQALSEFSQAANERSLLNSVTQLYLSKLKFGTDAVHLKSFYKPYLDVLPLHLPQPYFWSTDEVMNLHGTDVYLTMRDTLNKLVKEWRMLFQALSIEHSSQDKQFLSLFQENKDSAVVPLEQFCAHINGCKLEDSEWNSFVAYLWSYCIFNSRAFPRVILGRAGTDRTNLNEGFLYPIVDLLNHKNDVPVRWEMNEQNELCFMSQTTTFSAQDELFNNYGNISNEKCLLNYGFWDSSNKFDFSRLTLKLPSTLVSGLPVDFNKSGNFVTDDGETTILQFSLKISEPLPPVLLALFAYLSKLKSEETPTVRSVLEGIDQLTSVVSQRLLFYKNFKIKTSSTQKLRPHVIKLIKLYYQDNKKILNATTEKLSVLQKKIYSNNKEFSLSFKTIFKNDKIFANSLLLVFGAINYEDLITKDCLNDALLLWIVKLINDKSNNQGGFIKQTFKEVSDSIVIEKEDVMEFLPFYKKYFPNLSERIPEIYSVGDWGIRQFIVADTAIDRLVWIRKSNKEPIFLMKKAYDLQI
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that monomethylates elongation factor 1-alpha (TEF1/TEF2) at 'Lys-30'. Sequence Mass (Da): 67452 Sequence Length: 585 Subcellular Location: Cytoplasm EC: 2.1.1.-
P38347
MFDPLDLYTPDDIQVEALQFNLAEREPKDPCSPQRDEILTAVDEEESDDDDTIIDNLDLPSVKYAPPEVILCILILLKPDRQVNFNQETGKNKSVLEVCKSHGLEPDLLKRLLTWYTEEWPNKRLNSLEKICNKIPMLRFTVSKELLLGYYTSVLKKYNNSCGLNEEIIQELLKELSSRISENCGRTAQPSIVRYFELRNLSTSIPLHEPSLTADNLGWKTWGSSLILSQLVVDHLDYLHTTNVNMLANSDIKQIKVLELGAGTGLVGLSWALKWKELYGTENIEIFVTDLPEIVTNLKKNVSLNNLGDFVQAEILDWTNPHDFIDKFGHENEFDVILIADPIYSPQHPEWVVNMISKFLAASGTCHLEIPLRAKYAKEREVLKLLLKESDLKVVEERHSEGVDDWGAVKYLYRQIVRN
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono- and dimethylates elongation factor 2 (EFT1/EFT2) at 'Lys-613' and methylates elongation factor 3A (YEF3). Sequence Mass (Da): 47977 Sequence Length: 419 Subcellular Location: Cytoplasm EC: 2.1.1.-
P0CU27
MVASRNPQTARFCWQYLQLEQSLDFPDGELLRDEAVQETIYQQLFAPNAPTPLPPARYRLRVLKELTSRIESAIEDWETHGISDNLMDAMAELVAQPLPSEAEAAQERCYVTYYLSLLEGGLEKPHITLLESRSLISASGTTGLRTWEAALHLGQFLSVNSGLVKDKRVLELGTGTGYLAVLCAKYLGTSHVIASDGSEEVVEKLSDNLFVNGLQDSDKVQPMELKWGHALLGTEEEHWNGGRKIDVVLGADITYDVSVIPALIATLEELVDLYPGISIIIAATERNRETYETFLAACGRRGFSVTPESFPVPSRAEQKGPFYKDGTPIHICQLRR
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 2. Sequence Mass (Da): 37265 Sequence Length: 336 Subcellular Location: Cytoplasm EC: 2.1.1.-
O14118
MHGAPEFLTKVKQQYLQQVDLYRFQWVSKPTDWPILFNDYAQVFLSEIVTPSAYTRAFLKSYFRFLDSIDSGHNERNEALLYTYIESLSSTYIPPVQYSLGEYDILIRESRHVLLREGTTGARTWEAGMALAEYIYQHPVQSGMRVLELGAGTGLVSILCAKMGSIVLATDGDTKVCDGVRENARLNNCDINVKKLLWGVDPPEFSDIVFASDVTYDCDLRCLATTLTQIITINPNCKIILSASLRRQETFFNFLKLIQNLYARQLEVWDSPKILYYDSPPIVFYEVSK
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 2. Sequence Mass (Da): 33051 Sequence Length: 289 Subcellular Location: Cytoplasm EC: 2.1.1.-
P47163
MNEDLFYDRLHQRCPGKYLLEELETSKSNDVLHASRFVCEMELVQKTNAYYCKTIVKMLLDHEWIFAKAFTIVNDGEDEIEIYDYLYEKYIKLLSTGKPDPMMKDVVRYRFDEDVKIKIEETPNLISAASTTGFRTWEAALYMGDFLIHKPLQELAPVQGQDDGKKKLNVLEVGAGTGIVSLVILQKYHEFVNKMYVTDGDSNLVETQLKRNFELNNEVRENEPDIKLQRLWWGSDRVPEDIDLVVGADVTYDPTILPDLCECLAECLALDRCKLCLLSATIRSESTVQLFSQECNKLGLKCTIVTSTEYDANNEIRAMKALQFKPLIAPIRIYKITKQ
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono-, di- and trimethylates elongation factor 2 (EFT1/EFT2) at 'Lys-509'. Sequence Mass (Da): 39024 Sequence Length: 339 Subcellular Location: Cytoplasm EC: 2.1.1.-
Q9P7Z3
MSGLPESKLGTKQYWDNVYEREVSNFTEFNDEGEVWFGEEAEERIVQWLEDHISTSFREVSEAAPFRVLDLGTGNGHLLFRLLEEEDTLLPSPCQLVGVDYSEAAIVLAKNIARHRQFSDKVKFQQLDIIKDSKFCSKDWDLILDKGTFDAISLSGELLDGRPLNSVYVDRVRGMLSPNGIFLITSCNWTIQELEERFTKNGFIVHSTVPVPVFEFQGSTGSSTSVIAFQIDPSFNRK
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono- and dimethylates elongation factor 1-alpha at 'Lys-316'. May play a role in intracellular transport. Sequence Mass (Da): 27011 Sequence Length: 238 Subcellular Location: Cytoplasm EC: 2.1.1.-
P40516
MKRSEKKSMSSALKNGIMERTQPEKVVQMQGTADLSTSKLGTKKYWDELYALELENFRRNPQDTGDCWFSDSDAEQKMIDFLVDNIGAYRISENASVVDLGTGNGHMLFELHQTEFQGKLVGIDYSEESVKLASNIAEATGVDNFISFQQADIFSGDWKPGKYDIVLDKGTLDAISLSGMKINGKLDVVDVYAGVVERILKKDGIFLITSCNFTQDELVKIIETDNLKMWKTIKYPVFQFGGVQGATICSVAFVKQN
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono- and dimethylates elongation factor 1-alpha (TEF1 and TEF2) at 'Lys-316'. May play a role in intracellular transport. Sequence Mass (Da): 28690 Sequence Length: 257 Subcellular Location: Cytoplasm EC: 2.1.1.-
Q5WRN3
MSDTDDIPQLSADTLAALSMFQAEQQEKIEQLQSGIIEKIDEDWQLSQFWYDDETSRKLVAEGVAAALEGSEARPARIGCVSSPTLVKFFHETEEYKTGQIQLTLFEFDDRFGLKFPTEFVHYDYKHPTDLPAELLAKFDVIIADPPFLAAECLIKTAHSIRLLGKSDVKVLLCTGAIMEDYASRLMAMHRTSFEPRHANNLANDFSCFANYQTLTFC
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha. Sequence Mass (Da): 24660 Sequence Length: 218 Subcellular Location: Cytoplasm EC: 2.1.1.-
Q86A24
MSDSSDDEITLSKESLSALQDFYKSREVEQQDKFEISEDWQLSQFWYEEETSKFVANVIEQETIGGNVVVCLSTPSIYKVLHKNNNLLLNNNLFEYDKRFDVYGEKFHFYDYNNPEDGISEQLKGNVDYICLDPPFLSEECIEKVAKTIALLRKPTTRLLLLTGRIQWNNIQKYLPEMMICEFEPKHPRLQNDFFCCSNYHSKLLGLENKK
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha. Sequence Mass (Da): 24823 Sequence Length: 211 Subcellular Location: Cytoplasm EC: 2.1.1.-
Q9VMH7
MDDDISLPADTLAILNEFLLERSKREAEEENQIANKTGKDAQFEEDWQLSQFWYSTETKHALRDVVRKLLAERTEDSGDFSIALLSCPSLYKDIREIHDTVHIFEFDKRFEAYGTDFVHYDLNCVGSNPDYLKEHHQQYDLIVADPPFLSQECIAKTCEIITRLQRNQKESKVILCSGEVVEPWLTARLPVLKCSFRPEHERNLGNKFVSYANFNLDEYIENK
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha. Sequence Mass (Da): 26043 Sequence Length: 223 Subcellular Location: Cytoplasm EC: 2.1.1.-
P53200
MSDSDSDSDYELTLSANALAALEEFKREEQQHQEAFQKLYDETDEDFQKKKKEEGMKLFKEDWQLSQFWYSDDTAAILADAILEGADENTVIAIVSAPSVYAAIQKKPTNEIPTEHIYLFEFDKRFELLAGRDHFFFYDYNKPLDFSDEIKGKVDRLLIDPPFLNEDCQTKSSITAKCLLAPNDNSKTKKGVFKHRLISCTGERMSEVISKVYSDTRITTFLPEHSNGLSNEFRCYANFECSSWKFAS
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that trimethylates elongation factor 1-alpha (TEF1 and TEF2) at 'Lys-79' . Required for replication of Brome mosaic virus (BMV) . Sequence Mass (Da): 28582 Sequence Length: 248 Subcellular Location: Cytoplasm EC: 2.1.1.-
P53970
MESIFGGFGDLVVPRPKEHLGQTDLSFGGKLLPALKICEDGGESGCGGKVWIAGELLCEYILEKSVDHLLSKTVNGTKQFKKVLELGSGTGLVGLCVGLLEKNTFHDGTKVYVTDIDKLIPLLKRNIELDEVQYEVLARELWWGEPLSADFSPQEGAMQANNVDLVLAADCVYLEEAFPLLEKTLLDLTHCINPPVILMAYKKRRKADKHFFNKIKRNFDVLEITDFSKFEHYLKERTHLFQLIRK
Function: S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha (TEF1 and TEF2) at 'Lys-390'. Sequence Mass (Da): 27738 Sequence Length: 246 Subcellular Location: Cytoplasm EC: 2.1.1.-
P49778
MISVNDFRTGLTIEVDGGIWRVVDFQHVKPGKGAAFVRSKLRNLRTGAIQEKTFRAGEKVAKAQIETKTMQYLYANGDQHVFMDTSSYEQLELSATQIEEELKYLLENMSVHIMMYQDETLGIELPNTVELKVVETEPGIKGDTASGGTKPAKTETGLVVNVPFFVNEGDTLVVNTSDGSYVSRA
Function: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase (By similarity). Sequence Mass (Da): 20468 Sequence Length: 185 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Cytoplasm
Q8A1F7
MINAQDIKNGTCIRMDGKLYFCIEFLHVKPGKGNTFMRTKLKDVVSGYVLERRFNIGEKLEDVRVERRPYQFLYKEGEDYIFMNQETFDQHPIAHDLINGVDFLLEGAVLDVVSDASTETVLYADMPIKVQMKVTYTEPGMKGDTATNTLKPATVESGATVRVPLFISEGETIEIDTRDGSYVGRVKA
Function: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. Sequence Mass (Da): 21227 Sequence Length: 188 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Cytoplasm
A1UR93
MKINGNEIRPGNVIEHQGSLWVAVKCNAVKPGKGGAFNQVELKNVIDGTKLNERFRAAETVEKVRLEQKDFTFLYQQGEALVFMDTESYEQLELQRDFVGDRAAFLQDGMTVTVELHEEKPLGISLPDQVTVTIAEADPAIKGQTVTSSYKPAILENGIRILVPPFVQAGERIIVDTNELTYIRRVSEKG
Function: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. Sequence Mass (Da): 21175 Sequence Length: 190 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Cytoplasm
B2KCP2
MISTTDFKEGLIFENENGEIVEIVDYQHHRKSQARAVVRVKLRKLGSGSYVETSYRPEDKFKEVSVEKRPFMYLYSEGDMAHFMNNESYDQVAVPLDKLENQRKYLIENMECTGLYINDQLFDIVLPIKVVLTIKSTVPGVKGDTVSNLTKEAELETGVTIKVPLFINEGDKVIMDTRYCTYVERA
Function: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. Sequence Mass (Da): 21388 Sequence Length: 186 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Cytoplasm
B1GZJ0
MISTSDFKNGTNILVDGEPYQIAWFQNHKPGKGGAVMRVKLRHLKKGGIIERTFKSGEKFKALTITRQKKRFLYKESNNFNFMDMDTYEQITVHPELLGKMVNFLKENLEVEAIYLENELIGIDLPVIIEMTIAEAEHGIKGDSVSNTTKTAKLETGADIHVPLFIKEGDRIKVDTRTGEYVERA
Function: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. Sequence Mass (Da): 21033 Sequence Length: 185 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Cytoplasm
Q2NAZ6
MKISGVDIRPGNIIEYEGGIWKVAKIQHTQPGKGGAYMQVEMKNLQDGRKTNVRFRSADTVEKVRLDTQDYQFLYEDGDQLVFMDQDTYEQINLDSDLLGDARPFLQDGMTVQLELWEEKPISVQLPQQVEADIVEADAVVKGQTASSSYKPAVLDNGVRIMVPPHIESGTRIVVDVYEQTYVGKAG
Function: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. Sequence Mass (Da): 20967 Sequence Length: 187 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Cytoplasm
Q885R6
MKTGKELKPGTVIRLENDPWLVQKAEFTKSGRNSAIMKTKLKNLLTGYKTEIVYSADDKLDDVILDRKEATLSFISGDTYTFMDTTDYTMYELNAEDIESVLPFVEEGMTDVCEAVFFDERLVSVELPTTIVRQVDYTEGSARGDTSGKVMKPAKLKNGTELSVADFIEIGDMIEIDTREGGSYKGRAK
Function: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. Sequence Mass (Da): 21138 Sequence Length: 189 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Cytoplasm
Q8D2R7
MIIYYSANQSKSGLKILLKNEPCLILENQFVKPGKGQPFNRIKIKKLISGKIFTKIFKSNEKLIYADVLDVKVKSLYKDKKYWNFIKKENFEQFKISKKNLGEKYKWIIEQLECIVTFWDENPINITLPRFVDIKVCNANFDIKGDTIKSGNKYIILTTGAIIKAPIFIRSEEIVRVDTNLGEYVSRIK
Function: Involved in peptide bond synthesis. Alleviates ribosome stalling that occurs when 3 or more consecutive Pro residues or the sequence PPG is present in a protein, possibly by augmenting the peptidyl transferase activity of the ribosome. Modification of Lys-35 is required for alleviation. PTM: May be beta-lysylated on the epsilon-amino group of Lys-35 by the combined action of EpmA and EpmB, and then hydroxylated on the C5 position of the same residue by EpmC (if this protein is present). Lysylation is critical for the stimulatory effect of EF-P on peptide-bond formation. The lysylation moiety may extend toward the peptidyltransferase center and stabilize the terminal 3-CCA end of the tRNA. Hydroxylation of the C5 position on Lys-35 may allow additional potential stabilizing hydrogen-bond interactions with the P-tRNA. Sequence Mass (Da): 22050 Sequence Length: 189 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Cytoplasm
Q73FP5
MAERANDIRPGQVLEHNGGLFLVVGIMHTQPGKGGAYIQAEMKNIKTGAKHYERFRSDATIRRAILDEEEYVYLFTEGNIVNLMHPSNYEQITINLDLLGEKKIYLQDNMKIKVVAYQDKIISAHVPDYVTLAVKETESVIKGQTATASYKPAILENGMRVNVPQFIKEEDKIVVYTPGDSYYERVKE
Function: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. Sequence Mass (Da): 21353 Sequence Length: 188 Pathway: Protein biosynthesis; polypeptide chain elongation. Subcellular Location: Cytoplasm
Q0C7P6
MQLTQLLALALSLATSEAAYKGFNYGDKKPDGSSKYQADFASEFETAQNLVGAPGFTSARLYTMIQAGTANDPISAIPAAIAQNTSLLLGLWASGNNMNNELTALKAAISQYGEDLSKLVVGISVGSEDLYRNSVLGQKVNAGVGVDPHVLASYIEEVRSTISGTPLSGAPLGHVDTWNDWVNGSNAAVIDAVDWVGFDGYPYFQNTMANSIDDAKALFNEAVAKTKSAAGNKEVWITETGWPVSGKTENLAVASIPNAKRFWDEVGCPLFDNTNTWWYTLQDAFGASVPNPSFGIVGSTLTTQPLFDLSCSKSNTTSSSAIASPTSTAAAAGGVAGGSTGSASGSSTGTGSSSGSGSNSNTGAASGAVGAADRETSGTSGSANTNGTSGSGSGSNSTSGHGSNVTVPTRPTSVSNVSPSKSSSALFTGAATSMGASPSSVGNVGPSKSSGAASPSSTTMFTGAATSVSAPVVHVVLLALMMVIAA
Function: Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase (By similarity). PTM: The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By similarity). Location Topology: Lipid-anchor Catalytic Activity: Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans. Sequence Mass (Da): 48753 Sequence Length: 486 Subcellular Location: Cell membrane EC: 3.2.1.39
Q5AUT0
MFTKTQILALALSIASAEAVSKGFNYGANKPDGTLKVQADFEAEFRTAKNLETTSGFNSARLYTMIQGTGSTPISAIPAAIAEETTLLLGLWASGGNMDNEIAALKAAINQYGDEFAKLVVGISVGSEDLYRNSEIGVQANAGIGIEPEELVSYIQRVREAIAGTALSGAPIGHVDTWNAWTNGSNAAVAEAVDWLGFDGYPFFQNTMQNSIDDAKALFDESVQKTKAVAGNKEVWITETGWPVSGDSQNLAIASVENAKQFWDEVGCPLFDNVNTWWYILQDASGSSVPNPSFGIVGNTLSTTPLFDLSCSASSKKNSSSASASASGSSAQSTGFVSTTKPAASPSGSSGLGHGGSLGSSGSFSGGHYAGVGSSSVIASPSATPSGSAVPGSSSGPGSSSGSASGSSSGFGSGAAADSTSGTSTSGDSTSSTSATPADFTGAGSRLSGSIFGAAMLVAALAVAL
Function: Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase. PTM: The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By similarity). Location Topology: Lipid-anchor Catalytic Activity: Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans. Sequence Mass (Da): 46685 Sequence Length: 465 Subcellular Location: Cell membrane EC: 3.2.1.39
A1DJ47
MQFTQLVALALALATSEAAHQGFNYGNTKSDGSAKSQSDFQAEFSTAKNLVGTSGFTSARLYTMIQGGTANTPISAIPAAVAEETSLLLGLWASGGNFANEIAALKTAIADYGDDLAKLVVGISVGSEDLYRNSVDGVKAKAGLGTNPDEIVSYINQVRSTIAGTKLSGAPIGHVDTWTAWVNGSNSAVIDACDWLGFDGYPYFQNTMANSISDAKALFDESVAKTEAVAKGKEVWITETGWPVSGNTENLAVANLANAKTYWDEVGCPLFGKTNTWWYILQDANPVTPNPSFGIVGSTLSTTPLFDLSCSASSSAAASSTAVPSASSVAGAKASGFATAAASSGAAGSAKPTFTVGKGPGGSYNGTGFWNSTSSARPSSTAISGSSSGSASGSSGSSGSGASGASGQSSSSTGSSSAPSSSNILSNAASGLSGSIFGAVVAVCLALAAL
Function: Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase (By similarity). PTM: The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By similarity). Location Topology: Lipid-anchor Catalytic Activity: Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans. Sequence Mass (Da): 44878 Sequence Length: 450 Subcellular Location: Cell membrane EC: 3.2.1.39
Q5BCX8
MKFSSVLALAASAKLVASHATVFAVWINDEDQGLGNTADGYIRTPPNNSPVTDVTSTDLTCNVNGDQAAAKTLEVAAGDKITFEWHHNSRDSSDDIIADSHKGPVLVYMAPTEAGSAGKNWVKIYEDGYNDGTWAVDTLIANKGKHSVTVPDVPAGNYLFRPEIIALHEGNREGGAQLYMECVQFKVTSDGTTQLPEGVSLPGAYTATDEGILFDIYSSFDSYPIPGPAVWDGASSGSGSSGSGSSSSAAATSSAEKTATSTTAAATTTAVATSTSSATQVQPTSVATFTTSVRPTTSAAPTTSAPTSSAAPTGGTGTGSIQIYQQCGGMNYKGATGCASGLTCKQWNPYYHQCVQA
Function: Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates. Catalytic Activity: Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Sequence Mass (Da): 36732 Sequence Length: 357 Domain: Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM). The genes for catalytic modules and CBMs seem to have evolved separately and have been linked by gene fusion. Subcellular Location: Secreted EC: 3.2.1.4
Q9GZT9
MANDSGGPGGPSPSERDRQYCELCGKMENLLRCSRCRSSFYCCKEHQRQDWKKHKLVCQGSEGALGHGVGPHQHSGPAPPAAVPPPRAGAREPRKAAARRDNASGDAAKGKVKAKPPADPAAAASPCRAAAGGQGSAVAAEAEPGKEEPPARSSLFQEKANLYPPSNTPGDALSPGGGLRPNGQTKPLPALKLALEYIVPCMNKHGICVVDDFLGKETGQQIGDEVRALHDTGKFTDGQLVSQKSDSSKDIRGDKITWIEGKEPGCETIGLLMSSMDDLIRHCNGKLGSYKINGRTKAMVACYPGNGTGYVRHVDNPNGDGRCVTCIYYLNKDWDAKVSGGILRIFPEGKAQFADIEPKFDRLLFFWSDRRNPHEVQPAYATRYAITVWYFDADERARAKVKYLTGEKGVRVELNKPSDSVGKDVF
Cofactor: Binds 1 Fe(2+) ion per subunit. Function: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif. PTM: S-nitrosylation inhibits the enzyme activity up to 60% under aerobic conditions. Chelation of Fe(2+) has no effect on the S-nitrosylation. It is uncertain whether nitrosylation occurs on Cys-323 or Cys-326. Catalytic Activity: 2-oxoglutarate + L-prolyl-[hypoxia-inducible factor alpha subunit] + O2 = CO2 + succinate + trans-4-hydroxy-L-prolyl-[hypoxia-inducible factor alpha subunit] Sequence Mass (Da): 46021 Sequence Length: 426 Domain: The beta(2)beta(3) 'finger-like' loop domain is important for substrate (HIFs' CODD/NODD) selectivity. Subcellular Location: Cytoplasm EC: 1.14.11.29
Q96KS0
MDSPCQPQPLSQALPQLPGSSSEPLEPEPGRARMGVESYLPCPLLPSYHCPGVPSEASAGSGTPRATATSTTASPLRDGFGGQDGGELRPLQSEGAAALVTKGCQRLAAQGARPEAPKRKWAEDGGDAPSPSKRPWARQENQEAEREGGMSCSCSSGSGEASAGLMEEALPSAPERLALDYIVPCMRYYGICVKDSFLGAALGGRVLAEVEALKRGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGSYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAAKDKYQLASGQKGVQVPVSQPPTPT
Cofactor: Binds 1 Fe(2+) ion per subunit. Function: Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as ATF4, IKBKB, CEP192 and HIF1A . Target proteins are preferentially recognized via a LXXLAP motif . Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins . Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A . Also hydroxylates HIF2A . Has a preference for the CODD site for both HIF1A and HIF2A . Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex . Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes . EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle . Also regulates susceptibility to normoxic oxidative neuronal death . Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation . Hydroxylates IKBKB, mediating NF-kappa-B activation in hypoxic conditions . Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4 (By similarity). PTM: Ubiquitinated by SIAH1 and/or SIAH2 in response to the unfolded protein response (UPR), leading to its degradation. Catalytic Activity: 2-oxoglutarate + L-prolyl-[protein] + O2 = CO2 + succinate + trans-4-hydroxy-L-prolyl-[protein] Sequence Mass (Da): 43650 Sequence Length: 407 Domain: The Beta(2)beta(3) 'finger-like' loop domain is important for substrate (HIFs' CODD/NODD) selectivity. Subcellular Location: Nucleus EC: 1.14.11.-
Q91YE2
MDSPCQPQALNQALPQLPGSVSESLESSRARMGVESYLPCPLLPAYHRPGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGMGCDSGASNSSSSSSNTTSSSGEASARLREEVQPSAPERLALDYIVPCMRYYGICVKDNFLGAVLGGRVLAEVEALKWGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGNYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPTTPT
Cofactor: Binds 1 Fe(2+) ion per subunit. Function: Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as ATF4, IKBKB, CEP192 and HIF1A . Target proteins are preferentially recognized via a LXXLAP motif (By similarity). Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins . Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A . Also hydroxylates HIF2A . Has a preference for the CODD site for both HIF1A and HIF2A . Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex . Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes . EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle . Also regulates susceptibility to normoxic oxidative neuronal death . Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation (By similarity). Hydroxylates IKBKB, mediating NF-kappa-B activation in hypoxic conditions (By similarity). Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4 . PTM: Ubiquitinated by SIAH1 and/or SIAH2 in response to the unfolded protein response (UPR), leading to its degradation. Catalytic Activity: 2-oxoglutarate + L-prolyl-[protein] + O2 = CO2 + succinate + trans-4-hydroxy-L-prolyl-[protein] Sequence Mass (Da): 45109 Sequence Length: 419 Domain: The Beta(2)beta(3) 'finger-like' loop domain is important for substrate (HIFs' CODD/NODD) selectivity. Subcellular Location: Nucleus EC: 1.14.11.-
Q7QBW3
MSDTISCTPETATFEAPQLQFNEDGWGPCELPDAFKDIPYQPFSKSDRLGKISDWTGTAQTDKKFSNKYASQFGGGSQYAYFHEEDETTFHLVDSARIQKPPHQRGRFRGNMRNNRSGRGRGARGGVQVGGMTTLSKSANKMRDQRRGTTRKWGMRGPPPKIRDASVTVRPDWVTIEEMDFPRLAKLSLPSVKEGEDIMTCGTLEYYDKTYDRVNVKNERPLQSVDRIFHTVTTTDDPIIRQLSKTHGNVYATDAILATIMCCTRSNYSWDIVIDKIGGKLFMDKRDNTEFDLLTVNETASEPPQEDGNSLNSPRNLAIEATFINHNFSQQVLKSGEKEPKYKFQQPNPFIGDDEDGEVASVAYRYRKWDLNNGITLVARCEHDAVLKMPQGDTQFLTIKALNEWDSKIANAVEWRQKLDTQRGAILANELRNNSCKLAKWTVQALLAGSDQLKFGYVSRAHVRDSSKHVILGTQQFKPQEFANQINLSMDNAWGILRCIIDICMKQKDGKYLIMKDPNKPMVRLYDIPDNTFDSDGEGEEGDDGEAFQPMYGYSAAASTSANVTAAQSNTSATVPDQSEKASA
Function: mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In the eIF-3 complex, eif3d specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs. Sequence Mass (Da): 65600 Sequence Length: 584 Domain: The RNA gate region regulates mRNA cap recognition to prevent promiscuous mRNA-binding before assembly of eif3d into the full eukaryotic translation initiation factor 3 (eIF-3) complex. Subcellular Location: Cytoplasm
P56820
MVTEAFEFVAVPFNSDGWGPPDASDVSSSASPTSVAAANLLPNVPFASFSRSDKLGRVADWTRNLSNPSARPNTGSKSDPSAVFDFSAFAIDEGFGLASSGGNPDEDAAFRLVDGKPPPRPKFGPKWRFNPHHNRNQLPQRRDEEVEAKKRDAEKERARRDRLYNNNRNNIHHQRREAAAFKSSVDIQPEWNMLEQIPFSTFSKLSYTVQEPEDLLLCGGLEYYNRLFDRITPKNERRLERFKNRNFFKVTTSDDPVIRRLAKEDKATVFATDAILAALMCAPRSVYSWDIVIQRVGNKLFFDKRDGSQLDLLSVHETSQEPLPESKDDINSAHSLGVEAAYINQNFSQQVLVRDGKKETFDEANPFANEGEEIASVAYRYRRWKLDDNMHLVARCELQSVADLNNQRSFLTLNALNEFDPKYSGVDWRQKLETQRGAVLATELKNNGNKLAKWTAQALLANADMMKIGFVSRVHPRDHFNHVILSVLGYKPKDFAGQINLNTSNMWGIVKSIVDLCMKLSEGKYVLVKDPSKPQVRIYEVPPDAFENDYVEEPLPEDEQVQPTEENTEGAEASVAATKETEEKKADDAQA
Function: mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In the eIF-3 complex, eif3d specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs. Sequence Mass (Da): 66725 Sequence Length: 591 Domain: The RNA gate region regulates mRNA cap recognition to prevent promiscuous mRNA-binding before assembly of eif3d into the full eukaryotic translation initiation factor 3 (eIF-3) complex. Subcellular Location: Cytoplasm
Q3T122
MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYSSQFGGGSQYAYFHEEDETSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMLQFNLQTLPKSAKQKERERIRLQKKFQKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLMKMRYLEVSEPQDIECCGALEYYDKAFDRITTRSEKPLRSIKRIFHTVTTTDDPVIRKLAKTQGNVFATDAILATLMSCTRSVYSWDIVVQRVGSKLFFDKRDNSDFDLLTVSETANEPPQDEGNSFNSPRNLAMEATYINHNFSQQCLRMGKERYNFPNPNPFVEDDMDKNEIASVAYRYRRWKLGDDIDLIVRCEHDGVMTGANGEVSFINIKTLNEWDSRHCNGVDWRQKLDSQRGAVIATELKNNSYKLARWTCCALLAGSEYLKLGYVSRYHVKDSSRHVILGTQQFKPNEFASQINLSVENAWGILRCVIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSDEDDEEEEEEEEEEEEEEA
Function: mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs. Sequence Mass (Da): 63931 Sequence Length: 548 Domain: The RNA gate region regulates mRNA cap recognition to prevent promiscuous mRNA-binding before assembly of eif3d into the full eukaryotic translation initiation factor 3 (eIF-3) complex. Subcellular Location: Cytoplasm
P30642
MALPKFELLSLADNTVGWGPLASSSSADEPVPFQQFNKADRIGRVADWIGVDRFYRRGNERYNERVYGSAANAGSQFDYIHGMDEHNFQLVDTSKPMARNPQRNFRVRQMHLRKMMQKENEKREMVNQSTNLRMKRSIAKEQQRAFKMWQRRGGNARQGQRGQGGRFGGDRPKERLPSVQVRPEWVVLEEMNLSAFSKLALPNIPGGDDIGDHQYGSLQYYDKTIDRVSVKNSIPLQRCAGVFYNVTTTEDPVIQELAQGGAGNVFGTDIILATLMTAPRSVYSWDIVAYRVGDKLFFDKRNTRDILNPVETLTVSETSAEPPSFDGNGINNAKDLATEAFYINQNFRRQVVKRNDAGFTFKNARAPFEDEETGESGTAYKYRKWNLGNGVDGKPVELVCRTELDGVIHGLGNETQTLTIKAFNEWDSTQSGGVDWRTKLDVQKGAVMATEIKNNSAKVAKWTLQALLAGSDTMKLGYVSRNNARSTQNHSILLTQYVKPTEFASNIALNMDNCWGILRCVIDSCMKQKPGKYLLMKDPQSPVIRLYSLPEGTFESERESSDEENSDDDQ
Function: mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In the eIF-3 complex, eif3d specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs. Sequence Mass (Da): 64289 Sequence Length: 570 Domain: The RNA gate region regulates mRNA cap recognition to prevent promiscuous mRNA-binding before assembly of eif3d into the full eukaryotic translation initiation factor 3 (eIF-3) complex. Subcellular Location: Cytoplasm
Q9C5Z2
MATMARSFLQAISKDEAVAPPLRVVQIEGLAVLKIIKHCKEFSPTLVTGQLLGLDVGSVLEVTNCFPFPVRDDDEEIEADGANYQLEMMRCLREVNVDNNTVGWYQSTVLGSYQTVELIETFMNYQENIKRCVCIIYDPSKADLGVLALKALKLSDSFMELYRGGNFTGEKLREKNFSWMDIFEEIPIKVSNSALVSAFMTELETDTPVSQGDYDRLHSSTTPFLENNMEFLIKCMDDLSMEQQKFQYYYRNLSRQQAQQQAWLQKRRTENMARKSAGEEPLPEEDPSNPIFKAIPEPSRLESFLITNQVSNFCGQINGVAGQNFSRLYLTKALHDN
Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (Potential). Regulates translation initiation of specific 5' mRNAs harboring multiple upstream open reading frames (uORFs) in their 5' leader sequence (e.g. BETA-OHASE 2 and LHY) . PTM: In response to auxin (NAA), phosphorylated at Ser-178 by ATPK1 and binds to polysomes via TOR signaling. This phosphorylation is repressed by Torin-1. Sequence Mass (Da): 38373 Sequence Length: 337 Subcellular Location: Cytoplasm
P26378
MEWNGLKMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPSGATEPITVKFANNPSQKSSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRLMSGPVPPSACPPRFSPITIDGMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKAHKS
Function: RNA-binding protein that is involved in the post-transcriptional regulation of mRNAs . Plays a role in the regulation of mRNA stability, alternative splicing and translation . Binds to AU-rich element (ARE) sequences in the 3' untranslated region (UTR) of target mRNAs, including GAP43, VEGF, FOS, CDKN1A and ACHE mRNA . Many of the target mRNAs are coding for RNA-binding proteins, transcription factors and proteins involved in RNA processing and/or neuronal development and function (By similarity). By binding to the mRNA 3'UTR, decreases mRNA deadenylation and thereby contributes to the stabilization of mRNA molecules and their protection from decay . Also binds to the polyadenylated (poly(A)) tail in the 3'UTR of mRNA, thereby increasing its affinity for mRNA binding . Mainly plays a role in neuron-specific RNA processing by stabilization of mRNAs such as GAP43, ACHE and mRNAs of other neuronal proteins, thereby contributing to the differentiation of neural progenitor cells, nervous system development, learning and memory mechanisms . Involved in the negative regulation of the proliferative activity of neuronal stem cells and in the positive regulation of neuronal differentiation of neural progenitor cells (By similarity). Promotes neuronal differentiation of neural stem/progenitor cells in the adult subventricular zone of the hippocampus by binding to and stabilizing SATB1 mRNA (By similarity). Binds and stabilizes MSI1 mRNA in neural stem cells (By similarity). Exhibits increased binding to ACHE mRNA during neuronal differentiation, thereby stabilizing ACHE mRNA and enhancing its expression . Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). May bind to APP and BACE1 mRNAS and the BACE1AS lncRNA and enhance their stabilization . Plays a role in neurite outgrowth and in the establishment and maturation of dendritic arbors, thereby contributing to neocortical and hippocampal circuitry function (By similarity). Stabilizes GAP43 mRNA and protects it from decay during postembryonic development in the brain . By promoting the stabilization of GAP43 mRNA, plays a role in NGF-mediated neurite outgrowth (By similarity). Binds to BDNF long 3'UTR mRNA, thereby leading to its stabilization and increased dendritic translation after activation of PKC (By similarity). By increasing translation of BDNF after nerve injury, may contribute to nerve regeneration (By similarity). Acts as a stabilizing factor by binding to the 3'UTR of NOVA1 mRNA, thereby increasing its translation and enhancing its functional activity in neuron-specific splicing . Stimulates translation of mRNA in a poly(A)- and cap-dependent manner, possibly by associating with the EIF4F cap-binding complex (By similarity). May also negatively regulate translation by binding to the 5'UTR of Ins2 mRNA, thereby repressing its translation (By similarity). Upon glucose stimulation, Ins2 mRNA is released from ELAVL4 and translational inhibition is abolished (By similarity). Also plays a role in the regulation of alternative splicing . May regulate alternative splicing of CALCA pre-mRNA into Calcitonin and Calcitonin gene-related peptide 1 (CGRP) by competing with splicing regulator TIAR for binding to U-rich intronic sequences of CALCA pre-mRNA . PTM: Methylated by CARM1, which leads to reduced RNA-binding activity and enhanced interaction with SMN . Methylation at Arg-248 by CARM1 weakens protective binding to the 3'UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state (By similarity). Methylation is inhibited by NGF, which facilitates neurite outgrowth (By similarity). Sequence Mass (Da): 42398 Sequence Length: 385 Domain: The RRM 3 domain is required for binding to poly(A) RNA, for the association with polysomes and with the EIF4F cap-binding complex and for the stimulation of translation (By similarity). The RRM 1 and RRM 2 domains may contribute to polysome association and stimulation of translation (By similarity). Subcellular Location: Cytoplasm
O94533
MFQYEALHVGCNRIPTAATWSSNLGLIYGAERLIAVADPFKEINYLMAGHSGRINCVCELATNSEYRSPFILSGASDKTLRLWQLEEEYFTCIKTIELEATVNCLCVNENLVVCGCSNSSCIVYSWNAEQRNLTEISRFTCSEIIPLEFAIVKLDHGIILTVCGSSKKIMVYGSDSAISSFKLKAVLRGHLDWVRTLSFKKTSGSTATLASGSQDRYIRLWNISLWGSEDEKVSEEFFESVLSNKPVRFTLGKIDLKIVFDALLMGHEDWVMSVDWHPTKEMILSSSADSSMIVWEPDTNTGIWVVTGRMGEMASSHGSTTATGSAGGFWGGLWNPNGNCVVCWGRTGGWRLWKQDAGQWLQLPSISGHTKSVKGVAWDPEGKFYLSAGTDQTTRLFARFKKDNAWHEMARPQIHGYDLTSISCMPSRIGFLSCADEKVSRVFKFPKTIVRLLYRLCDTNIGEESLPDAANVPLLGLSNKATTASETGTVNAEEVQTPVADVIGSLNHPPFEEHLQRLLLFPEVEKLFGHGYEVYACAISNNGNIAATSCKSQTPEHAVIRLYETQSWNQQQVLKGHSLTVTTIKFSPDDRYILSAGRDRLVCLHEQAENLLDYNNFASIKAHSRIIWDASWAPKEMGYFFATASRDKFVKFWKINDNKKICDVAALQFSDAVTAVDFAPFFHNDELLLAVGTEAGKIFIWRCPRENLTKWYPTRLPDHMAPMESINQILWKPTFETMGLYSLLIAGEDTSVRLLNVTLG
Function: Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) . The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs . Sequence Mass (Da): 84949 Sequence Length: 760 Domain: Folds into a two seven-bladed beta-propeller structure which is required for elongator complex assembly. Pathway: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. Subcellular Location: Cytoplasm
Q5EBD9
MSAPIVQLAHAAFSVNRCPSCVSWSRTGILACATCHSIALYDPQKNVVISTLNGHTGRVNCVHWVQKNDCSAETELVSGGSDKKVIIWAIEKNKCEQPVAAEGHTEVVNAVHAVYTQRSENELLIVSAASDCTVRLWLRRNVKTECLQTLSFGKGFVLDVCISFLPGSLVPVLACGADDSRIHLYVLQNEQFEKTQVLHGHEDWIRGVEWAVTGQNLFLASCAQDCLIRIWKIFRKTAKENSKTEDENSIKLKENIFKVKEKDTETSYAVTLETVLTGHENSIYAVHWQPSFSRDGSIVQPMSLLSASMDKTMILWEPDEESGVWLEQVRVGEVGGNTLGFLGCQFSPDKSMILAHAFHGALHLWSRAPNKQNEWIPMVIISGHFDSVQDMRWDPDGQFVITVSADQTTRLFGPWKKKGQSHVTWHEIARPQIHGYDMQCLAMIGRFQFVSGADEKVLRVFAAPRNFIENFSNISCISVEKLLLNEDTNLPEGATVPALGLSNKAVFHGEILIQSTQEEGKYNSVSEQYSQPNFQPLNLTEPPTEDHLLQNTLWPEVQKLYGHGYEIFAVACNSAKTVIASACKASKREHAAIILWSTTSWKQLQSLSYHNLTVTQMAFSPDDEFLLVVSRDRNWSLWKKQEGIPEQTEPTFTLYASTDKNSCVHSRIIWACDWTPDSKYFVTGSRDKKVIVWGDCSLPSMNEEKSSALIRPCSSLLDTGDSITAVSVSQVLALDGSYIIAVGLDCGIIQLYKWKHSGTVNDWLKCLAMDQSLLSHTLTVKQLCWRSCLGRAGHDDRDNGDWIQLASCGADHCVKIFDVYRKAL
Function: Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. Sequence Mass (Da): 92123 Sequence Length: 824 Domain: Folds into a two seven-bladed beta-propeller structure which is required for elongator complex assembly. Pathway: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. Subcellular Location: Cytoplasm
P42935
MVECITPEAIFIGANKQTQVSDIHKVKKIVAFGAGKTIALWDPIEPNNKGVYATLKGHEAEVTCVRFVPDSDFMVSASEDHHVKIWKFTDYSHLQCIQTIQHYSKTIVALSALPSLISVGCADGTISIWRQNIQNDEFGLAHEFTIKKGFFYPLCLSLSKVEEKKYLLAIGGTNVNVFIASFILSDSGIEKCRVVAELEGHEDWVKSLAFRHQETPGDYLLCSGSQDRYIRLWRIRINDLIDDSEEDSKKLTLLSNKQYKFQIDDELRVGINFEALIMGHDDWISSLQWHESRLQLLAATADTSLMVWEPDETSGIWVCSLRLGEMSSKGASTATGSSGGFWSCLWFTHERMDFFLTNGKTGSWRMWATKDNIICDQRLGISGATKDVTDIAWSPSGEYLLATSLDQTTRLFAPWIYDASGRKREIATWHEFSRPQIHGYDMICVETVTDTRFVSGGDEKILRSFDLPKGVAGMLQKFVGIQFEEKSEMPDSATVPVLGLSNKAGEDDANEDDEEEEGGNKETPDITDPLSLLECPPMEDQLQRHLLWPEVEKLYGHGFEITCLDISPDQKLIASACRSNNVQNAVIRIFSTENWLEIKPALPFHSLTITRLKFSKDGKFLLSVCRDRKWALWERNMEDNTFELRFKNEKPHTRIIWDADWAPLEFGNVFVTASRDKTVKVWRHQKEPADDYVLEASIKHTKAVTAISIHDSMIREKILISVGLENGEIYLYSYTLGKFELITQLNEDITPADKITRLRWSHLKRNGKLFLGVGSSDLSTRIYSLAYE
Function: Component of the elongator complex, a multiprotein complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) . The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs . It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin) . May also be involved in sensitivity to Pichia inositovora toxin . ELP2 binds to microtubules . Independently, ELP2 may be involved in polarized exocytosis . Sequence Mass (Da): 89411 Sequence Length: 788 Domain: Folds into a two seven-bladed beta-propeller structure which is required for elongator complex assembly and association with microtubules. Pathway: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. Subcellular Location: Cytoplasm
Q23651
MDQKGSQRALLAQTINEIVKLLIEAHNQKKDVNLNRLKCIVAQKNGLSFQPKLVDIIAGVPSDYKDSLLPKLKAKPVRTASGIAVVAVMSKPHRCPHINFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYNPYLQTRGRLNQLMQLGHSVDKVEFIVMGGTFMSLPEDYRDFFIRNLHDALSGHTSASVEEAVAYSERSKMKCIGITIETRPDYCLPRHLNDMLLYGCTRLEIGVQSTYEDVARDTNRGHTVKSVCETFHMAKDTGYKVVIHMMPDLPNVGLERDKEQFLELFESPAFRPDGLKLYPTLVIRGTGLYELWKTGRYQSYPPSVLVDLIATILSLVPPWTRVYRVQRDIPMPLVSSGVEHGNLREHAMAKMKELGLKCRDVRTREVGIQEIHNKVRPEDVELIRRDYTANGGWETFISYEDPKQDILIGLLRLRKISDKAHRPELKGNVSVVRELHVYGSVVSVADRDPKKFQHQGYGSLLMEEAERIAREEHGSDKIAVISGVGTREYYRKLGYELDGPYMSKMLDSAAA
Cofactor: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. Function: Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (By similarity). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). Catalytic Activity: acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + 5-(carboxymethyl)uridine(34) in tRNA + CoA + 2 H(+) + L-methionine Sequence Mass (Da): 61910 Sequence Length: 547 Pathway: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. EC: 2.3.1.-
A0A1C7D1B7
MKKLSRTISGVTPVAVMTKPLPCPGKCIYCPTFAATPQSYTPESPAVLRAKSCEYQAYKQVALRLRIIQDMGHPTDKVELIIMGGTFLSADITYQYGFIKDCYDALNGVVAGSLEEAKTINETAQHRCVGLCIETRPDICGKAEIQRMIDFGTTRVELGVQMLDDDIYKLVERGHRVSDVAEATCLLREYGLKVHYHWMPGLPGSSPEKDLALSRMVFEDPRFCPDGLKLYPTMVVEGTILEQWWKEGRYTPYPNGTMTGLIADIKALVPPYVRISRVLRDIPAVFISAGLKDSLRDGVRQILESRHQKCRCIRCREYGHRQRKGQTSGEPTLRRLDYPASGGKEIFLSFEDASDTLYGLLRLRIPCASLPVLGQKYGAKTGLVRELHVYGTELSLGEQGDQSAQHRGLGRKLLAEAECLARDEFGLDSLAILSGVGAREYYRSLGYELVAGYMCKHLD
Cofactor: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. Function: tRNA uridine(34) acetyltransferase, which mediates formation of carboxymethyluridine in the wobble base at position 34 in tRNAs . The proposed mechanism is the following: (i) recruits S-adenosyl-L-methionine and cleaves it to generate a 5'-deoxyadenosine radical (5'-dA) in the radical S-adenosyl-L-methionine (rSAM) region, (ii) hydrolyzes acetyl-CoA in the N-acetyltransferase domain and (iii) an acetyl radical is formed by the products of the two domains and (iv) is transferred onto the C5 position of uridine(34) in the bound tRNA molecule. Does not show protein lysine acetyltransferase activity (By similarity). Catalytic Activity: acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + 5-(carboxymethyl)uridine(34) in tRNA + CoA + 2 H(+) + L-methionine Sequence Mass (Da): 51188 Sequence Length: 459 Pathway: tRNA modification. EC: 2.3.1.-
Q1ZXC6
MSLMNDPRPVSKKAGAKPVYGKNTPQFTKTVGEIVNALINAYKEGKKVNLLKIKTELAAKNSLSDQPKSVDIISAIPESYKNTLLPLLKAKPVRTASGIAVVAVMCKPHRCPHLAMTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYNPFLQTRHRIDQLKRLGHNVEKVEFIIMGGTFMSLPSDYRDYFIRNLHDALSGHTSNNVAEAVKYSEQSNVKCVGITIETRPDHCLKLHLSNMLTYGCTRLEIGVQSVFEDIARDTNRGHTVRAVLESFQLAKDSGFKVVAHMMPDLPNMGMERDIYGFMEFFENPAFRADGLKIYPTLVIRGTGLYELWKTGTYKNYSPDSLVDLIAKVLALVPPWTRIYRIQRDIPMPLVTSGVEYGNLRELCLARMKDFGTKCRDVRTREVGIQEVHHKIKPDQVELIRRDYVANGGWETFLSFEDPKQDILIGLLRLRKCSETSFRPELKENCSIVRELHVYGSVVGIHNRDPTKFQHQGYGTLLMEEAERIAREEHGSIKLAVIAGVGTRHYYRKLGYELDGVYVSKYLG
Cofactor: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. Function: Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (By similarity). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). Catalytic Activity: acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + 5-(carboxymethyl)uridine(34) in tRNA + CoA + 2 H(+) + L-methionine Sequence Mass (Da): 63210 Sequence Length: 559 Pathway: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. EC: 2.3.1.-
Q9H9T3
MRQKRKGDLSPAELMMLTIGDVIKQLIEAHEQGKDIDLNKVKTKTAAKYGLSAQPRLVDIIAAVPPQYRKVLMPKLKAKPIRTASGIAVVAVMCKPHRCPHISFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRNLHDALSGHTSNNIYEAVKYSERSLTKCIGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAVCESFHLAKDSGFKVVAHMMPDLPNVGLERDIEQFTEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYKSYSPSDLVELVARILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELALARMKDLGIQCRDVRTREVGIQEIHHKVRPYQVELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPTKFQHQGFGMLLMEEAERIAREEHGSGKIAVISGVGTRNYYRKIGYRLQGPYMVKMLK
Cofactor: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. Function: Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) . In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase . Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation . Required for acetylation of GJA1 in the developing cerebral cortex (By similarity). PTM: Tyrosine-phosphorylated; phosphorylation on Tyr-202 does not affect elongator complex integrity or ELP3 protein stability . Also serine/threonine-phosphorylated . Catalytic Activity: acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + 5-(carboxymethyl)uridine(34) in tRNA + CoA + 2 H(+) + L-methionine Sequence Mass (Da): 62259 Sequence Length: 547 Pathway: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. Subcellular Location: Cytoplasm EC: 2.3.1.-
D5VRB9
MKEKLMRCIIERILKEYKEGKTLDKKRIEQIKSECLRIYRIGIGHPSNSEILKYATEEEKKILIPILRKKPVRTISGVAVVAVMTSPAKCPHGKCIFCPGGLDSVFGDVPQSYTGREPATMRGLMFNFDPYLQTRARIEQLEKVGHPTDKIELIIMGGTFPAREIEYQDWFIKRCLDAMNERESKSLEEAQKINETAKHRCVALCIETRPDYCSEKEINQMLKLGATRVELGVQSIYNEILKLCKRGHSVEDTIKATQLLKDSGLKVSYHLMPGMPGSSIEMDKKMFKEIFTNPDFMPDMVKIYPCLVIEGTELYEMWKRGEFKPYREEEAIEVISYAKSIMPKWVRTSRIQRDIPATVIVDGVKKSNLGELVYKYMEKKGLRCRCIRCREVGHVYYKKGILPDPEHIKLVREDYEASGGTEIFLSFEDVKNDILIAFLRLRDPYKPFRKEIDDKTMLVRQLHVFGWEKALTRDIKEVSWQHMGYGRMLMKEAERIAKEEFGKKKILVTSGIGVREYYRKLGYKRVGAYMGKEL
Cofactor: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. Function: tRNA uridine(34) acetyltransferase, which mediates formation of carboxymethyluridine in the wobble base at position 34 in tRNAs . The proposed mechanism is the following: (i) recruits S-adenosyl-L-methionine and cleaves it to generate a 5'-deoxyadenosine radical (5'-dA) in the radical S-adenosyl-L-methionine (rSAM) region, (ii) hydrolyzes acetyl-CoA in the N-acetyltransferase domain and (iii) an acetyl radical is formed by the products of the two domains and (iv) is transferred onto the C5 position of uridine(34) in the bound tRNA molecule . Does not show protein lysine acetyltransferase activity . Catalytic Activity: acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + 5-(carboxymethyl)uridine(34) in tRNA + CoA + 2 H(+) + L-methionine Sequence Mass (Da): 61939 Sequence Length: 534 Pathway: tRNA modification. EC: 2.3.1.-
O14023
MSTSSLAFLKAKACAEIVAELIASENQNKVINLNALKMRISKKHQLSESPRLTDIIAAIPPDAYLKESLMRKLRAKPVRTASGIAVVAVMCKPHRCPHIAMTGNVCVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRVEQLRSLGHTVDKVEYIIMGGTFMSLPESYRHTFIANLHNALSGATTEDLDEAVKFSEQSETKCVGITIETRPDYCLDQHLDEMLRYGCTRLEIGVQSVYEDVARDTNRGHTVKAVCETFQLAKDTGYKVVTHMMPDLPNVGMERDIFQFQEYFENPAFRTDGLKLYPTLVIRGTGLYELWKTGRYKNYTPNALVDLIARILALVPPWTRIYRIQRDIPMPLVSSGVETGNLRELALNRMRDLGTKCRDIRAREVGMQEVHHKIHPEQVELLRRDYTANGGWETFLSYEDPKQDILIGLLRLRQCSDKTYRPEFTSQPTSLVRELHVYGSAVPVHSRDPKKFQHQGFGTLLLEEAERIAKYEHGSKKISVISGVGVRKYYQKLGYTLDGPYMSKWL
Cofactor: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. Function: Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) . In the elongator complex, acts as a tRNA uridine(34) acetyltransferase, which mediates formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). Catalytic Activity: acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + 5-(carboxymethyl)uridine(34) in tRNA + CoA + 2 H(+) + L-methionine Sequence Mass (Da): 61850 Sequence Length: 544 Pathway: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. Subcellular Location: Cytoplasm EC: 2.3.1.-
Q6GLC5
MAGVALKREGPQFISEAAVRGNAAVLDYCRTSVSALSGATAGILGLTALYGFIFYFLASFLLSLLLVLKSGRKWNKYFKSRKPLFTGGLIGGLFTYVLFWTFLYGMVHVY
Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes. Location Topology: Multi-pass membrane protein Sequence Mass (Da): 12090 Sequence Length: 110 Subcellular Location: Endoplasmic reticulum membrane
Q12431
MSSNEEVFTQINATANVVDNKKRLLFVQDSSALVLGLVAGFLQIESVHGFIWFLILYNLINVIYIVWICQLQPGKFYQSPLHDIFFESFFREITGFVMAWTFGYALIG
Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins . Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues . Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices . It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins (By similarity). Location Topology: Multi-pass membrane protein Sequence Mass (Da): 12404 Sequence Length: 108 Subcellular Location: Endoplasmic reticulum membrane
Q9NPA0
MAAALWGFFPVLLLLLLSGDVQSSEVPGAAAEGSGGSGVGIGDRFKIEGRAVVPGVKPQDWISAARVLVDGEEHVGFLKTDGSFVVHDIPSGSYVVEVVSPAYRFDPVRVDITSKGKMRARYVNYIKTSEVVRLPYPLQMKSSGPPSYFIKRESWGWTDFLMNPMVMMMVLPLLIFVLLPKVVNTSDPDMRREMEQSMNMLNSNHELPDVSEFMTRLFSSKSSGKSSSGSSKTGKSGAGKRR
Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins . Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues . Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices . It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes . By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors . By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). Location Topology: Single-pass type I membrane protein Sequence Mass (Da): 26471 Sequence Length: 242 Subcellular Location: Endoplasmic reticulum membrane
Q0IHY5
MPAVALGVSVGWRSLSLWLLALLQLCSADLDVLSPGSGSDKFKVEGRAVVPGVRPQDWVNTARVLVDGEEHVGFLRTDGSFVVHDVPSGSYVVEVISPAHRFEPVRVDITSKGKMRARYVNHIKTSEVVRLPYPLQMKSSGPPSYFIKRETWGWTDFLMNPMVMMMVLPLLIFVLLPKVVNTSDPEMRREMEQSMNMLNTNPELPDVSEFMTRLFTSKSSSKSSGSGKAGKSVGKRR
Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes. Location Topology: Single-pass type I membrane protein Sequence Mass (Da): 26341 Sequence Length: 237 Subcellular Location: Endoplasmic reticulum membrane
Q9W1Y1
MCDYKVSERAYAKLIFHAAKYPHQAVNGLLLAEKTSKGSQVEIVDAIPLFHQCLYVTPMAEVALMLIDAHAEREGLVIAGYYAAPENFYDNQVDKTPAAKIADKIQENFKNACFVVVDNKLMTLQHDRAAIQVFNCPGDSGARWSKAKFTLSQASDTLEGVSLLLKRGAMRDLVDFDNHLDNPDKNWTNDFLNQPLNDLQKLY
Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized multi-pass membrane proteins like rhodopsins. Location Topology: Peripheral membrane protein Sequence Mass (Da): 22781 Sequence Length: 203 Subcellular Location: Endoplasmic reticulum membrane
O43402
MPGVKLTTQAYCKMVLHGAKYPHCAVNGLLVAEKQKPRKEHLPLGGPGAHHTLFVDCIPLFHGTLALAPMLEVALTLIDSWCKDHSYVIAGYYQANERVKDASPNQVAEKVASRIAEGFSDTALIMVDNTKFTMDCVAPTIHVYEHHENRWRCRDPHHDYCEDWPEAQRISASLLDSRSYETLVDFDNHLDDIRNDWTNPEINKAVLHLC
Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins . Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues . Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices . It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes . By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors . By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). Location Topology: Peripheral membrane protein Sequence Mass (Da): 23773 Sequence Length: 210 Subcellular Location: Endoplasmic reticulum membrane
O70378
MPGVKLTTQAYCKMVLHGAKYPHCAVNGLLVAERQRPRKEHPPGAGSHTLFVDCIPLFHGTLALTPMLEVALTLIDSWCKDNSYVIAGYYQANERVKDASPNQVAEKVASRIAEGFGDAALIMVDNAKFTMDCAAPTIHVYEQHENRWRCRDPHHDYCEDWPEAQRISASLLDSRSYETLVDFDNHLDDIRSDWTNPEINKAVLHLC
Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes. Location Topology: Peripheral membrane protein Sequence Mass (Da): 23348 Sequence Length: 207 Subcellular Location: Endoplasmic reticulum membrane
Q9Y3B6
MGEVEISALAYVKMCLHAARYPHAAVNGLFLAPAPRSGECLCLTDCVPLFHSHLALSVMLEVALNQVDVWGAQAGLVVAGYYHANAAVNDQSPGPLALKIAGRIAEFFPDAVLIMLDNQKLVPQPRVPPVIVLENQGLRWVPKDKNLVMWRDWEESRQMVGALLEDRAHQHLVDFDCHLDDIRQDWTNQRLNTQITQWVGPTNGNGNA
Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins . Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues . Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices . It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes . By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors . By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). Location Topology: Peripheral membrane protein Sequence Mass (Da): 23061 Sequence Length: 208 Subcellular Location: Endoplasmic reticulum membrane
Q9WZP2
MEFLKRSFAPLTEKQWQEIDNRAREIFKTQLYGRKFVDVEGPYGWEYAAHPLGEVEVLSDENEVVKWGLRKSLPLIELRATFTLDLWELDNLERGKPNVDLSSLEETVRKVAEFEDEVIFRGCEKSGVKGLLSFEERKIECGSTPKDLLEAIVRALSIFSKDGIEGPYTLVINTDRWINFLKEEAGHYPLEKRVEECLRGGKIITTPRIEDALVVSERGGDFKLILGQDLSIGYEDREKDAVRLFITETFTFQVVNPEALILLKF
Cofactor: A flavin ligand is bound near the 3-fold axis channel; FMN is consistent with the observed density, absorbance data and mass spectrometry. Function: Shell component of a type 1 encapsulin nanocompartment. Assembles into proteinaceous shells 23-24 nm in diameter with 2-2.5 nm thick walls. Cargo protein Flp (ferritin-like protein, probably stores iron) is targeted to the interior via its C-terminal extension; empty intact shells can be isolated in the absence of cargo protein . Fe(2+) may be able to pass though the 5-fold and dimer channels in the protein shell (Probable). Sequence Mass (Da): 30478 Sequence Length: 265 Domain: Has 3 domains; a discontinuous peripheral domain (P, 13-39, 76-133, 221-254), an elongated loop (E, 53-73) and the discontinuous axial domain (A, 137-216 and 259-263). The E-loop forms contacts between two subunits, while the A domain mediates contacts in the 5-fold interface . Pores are formed by residues 184-189, pore size can be modified by mutagenesis . Subcellular Location: Encapsulin nanocompartment
Q4W945
MDQYSNLFAFEDYLGAQARSIPDLPEVDVLSPRVVRVLGGNPGQMQLQGTNTYILGTGAERLLIDSGQGRARWEQLMASLAAEHKFRISTVLLTHWHLDHTGGVPHLFRIFPELRGANAIYKYHPDPSQQAIVDGQVFSVEGATVRAVFTPGHSTDHMCFLLQEEEAIFTGDTVLGHGTTGVEDLEEYMQSLRKIQSLGCRIGYPGHGAVIENMQQKVQQEIDRKQRRERQVLLALQNIQREKRTVGDANGAATQAELIEAIFGRLPADVADRFFAPYMKDILMKMARDKQVGFRFKGGQKHWFANVSQENPVCR
Cofactor: Binds 2 Zn(2+) ions per subunit. Function: Atrochrysone carboxyl ACP thioesterase; part of the gene cluster that mediates the biosynthesis of endocrocin, a simple anthraquinone interesting for many biotechnological applications . The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) encA . The atrochrysone carboxyl ACP thioesterase encB then breaks the thioester bond and releases the atrochrysone carboxylic acid from encA . The atrochrysone carboxylic acid is then converted to endocrocin anthrone which is further oxidized into endocrocin by the anthrone oxygenase encC . The exact function of encD has not been identified yet, but it negatively regulates endocrocin production, likely through the modification of endocrocin itself . Catalytic Activity: atrochrysone carboxyl-[ACP] + H2O = atrochrysone carboxylate + H(+) + holo-[ACP] Sequence Mass (Da): 35346 Sequence Length: 315 EC: 3.1.2.-
A4DA85
MASVQGLIKIVAITGGVWLSGKITAHSLVSVPALLQTRSADGLSPCTILRVWRRIYEQGHRHSPQIAACTSTAFAYLAWCASDRTPRLLYGTAACSVMGIVPYTLLFMGPTNSRLLERSAAEEEKVPGATRGEDMVNVPSEMTTEELLSHWRFLAGIRGLLPLAGGILGLFAALYSNEGAR
Function: Anthrone oxygenase; part of the gene cluster that mediates the biosynthesis of endocrocin, a simple anthraquinone interesting for many biotechnological applications . The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) encA . The atrochrysone carboxyl ACP thioesterase encB then breaks the thioester bond and releases the atrochrysone carboxylic acid from encA . The atrochrysone carboxylic acid is then converted to endocrocin anthrone which is further oxidized into endocrocin by the anthrone oxygenase encC . The exact function of encD has not been identified yet, but it negatively regulates endocrocin production, likely through the modification of endocrocin itself . Location Topology: Multi-pass membrane protein Sequence Mass (Da): 19473 Sequence Length: 181 Subcellular Location: Membrane EC: 1.-.-.-
Q4W946
MTKPQPPPILDFSVFYGHDSQAKAQLVQRVRECCLNNGFFQITGHKVSPELQRRTFDCAKRFFDLPLIEKKKIERSPDAFNRGYEAFQSHMSQPGSAPDRKEGLFLGPDLAEDHPYCVQKKLNCGPNRWPQGLDDLEEFKLVSMEYYAALFQLAKDVVAVLALTMDYEETFFDPLTEGAIATLRYLHYPPQPVGDAEAGLGTGAHRDYSCITLLLQDGTGGLQVLDEPTGQWLDVKPVPGAYIVNLANVFARMTNGHYKSALHRVVNKSGMERYSIPFFFTGNPDYVCECLSRFRKEGEPVRHPPATVHEVVAEAVRGTVERANRYNAERQGIHAAQ
Cofactor: Binds 1 Fe(2+) ion per subunit. Function: 2-oxoglutarate-Fe(II) type oxidoreductase; part of the gene cluster that mediates the biosynthesis of endocrocin, a simple anthraquinone interesting for many biotechnological applications . The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) encA . The atrochrysone carboxyl ACP thioesterase encB then breaks the thioester bond and releases the atrochrysone carboxylic acid from encA . The atrochrysone carboxylic acid is then converted to endocrocin anthrone which is further oxidized into endocrocin by encC . The exact function of encD has not been identified yet, but it negatively regulates endocrocin production, likely through the modification of endocrocin itself . Sequence Mass (Da): 37912 Sequence Length: 337 Pathway: Secondary metabolite biosynthesis. EC: 1.14.11.-
Q8U1L4
MLSINPTLINRDKPYTKEELMEILRLAIIAELDAINLYEQMARYSEDENVRKILLDVAREEKAHVGEFMALLLNLDPEQVTELKGGFEEVKELTGIEAHINDNKKEESNVEYFEKLRSALLDGVNKGRSLLKHLPVTRIEGQSFRVDIIKFEDGVRVVKQEYKPIPLLKKKFYVGIRELNDGTYDVSIATKAGELLVKDEESLVIREILSTEGIKKMKLSSWDNPEEALNDLMNALQEASNASAGPFGLIINPKRYAKLLKIYEKSGKMLVEVLKEIFRGGIIVTLNIDENKVIIFANTPAVLDVVVGQDVTLQELGPEGDDVAFLVSEAIGIRIKNPEAIVVLE
Function: Fusion of the shell and cargo protein of a type 1 encapsulin nanocompartment . The nanocompartment is probably involved in iron storage (Probable). Expression in E.coli generates spherical particles (PfSPs) about 30 nm in diameter . The purified N-terminus has ferroxidase activity . Catalytic Activity: 4 Fe(2+) + 4 H(+) + O2 = 4 Fe(3+) + 2 H2O Sequence Mass (Da): 38802 Sequence Length: 345 Domain: The ferritin-like domain N-terminal 109 disordered residues are located inside the organelle in the crystal. The rest of the protein has 3 structural domains; a discontinuous peripheral domain (P, 110-138, 167-210, 304-334), an elongated loop (E, 139-166) and the discontinuous axial domain (A, 211-303 and 335-345). The E loop is highly flexible and allows formation of the pentamers and hexamers from the same protein . The N-terminal domain decamer has negatively charged patches on its exterior, and a negatively charged tunnel at the center, the charges are probably metal-binding sites . Subcellular Location: Encapsulin nanocompartment EC: 1.16.3.1
P46841
MTSAQNESQALGDLAAGQLANATKTVPQLSTITPRWLLHLLNWVPVEAGVYRVNRVVNPERVAVKAEAGAGTEAPLPETFVDYETSPREYTLRTISTLLDIHTRVSDLYSSPHDQITQQLRLTIETIKERQECELVNSPEFGLLAQVTPEQTIRTFAGAPTPDDLDALITKVWKMPSFFLTHPQGIAAFGREATYRGVPPVVVSLFGAQFITWRGIPLIPSDKVPVQDGETKFILVRTGEERQGVVGLFQPGLVGEQAPGLSVRFTGINQAAIATYLVTLYTSLAVLTDDALAVLDNVAVDQFHEYK
Function: Shell component of a type 2A encapsulin nanocompartment. Forms encapsulin nanocompartments about 24 nm in diameter from 60 monomers. Probably encapsulates at least cysteine desulfurase (CyD, AC O32975) and allows passage of cysteine into its interior, probably involved in sulfur metabolism (By similarity). Expression in M.smegmatis generates a multimeric protein, whereas expression in E.coli does not . PTM: The N-terminus is blocked. Location Topology: Peripheral membrane protein Sequence Mass (Da): 33651 Sequence Length: 307 Domain: Has 4 domains; an N-terminal arm not found in the type 1 subfamily, a discontinuous peripheral domain (P), an elongated loop (E) and the discontinuous axial domain (A). Subcellular Location: Encapsulin nanocompartment
I3NID5
MTSAQNESQALGDLAARQLANATKTVPQLSTITPRWLLHLLNWVPVEAGIYRVNRVVNPEQVAIKAEAGAGSEEPLPQTYVDYETSPREYTLRSISTLVDIHTRVSDLYSSPHDQIAQQLRLTIETIKERQELELINSPEYGLLAQATPEQTIQTLAGAPTPDDLDALITKVWKTPSFFLTHPLGIAAFGREATYRGVPPPVVSLFGAQFITWRGIPLIPSDKVPVEDGKTKFILVRTGEERQGVVGLFQPGLVGEQAPGLSVRFTGINQSAIATYLVTLYTSLAVLTDDALAVLDDVAVDQFHEYK
Function: Shell component of a type 2A encapsulin nanocompartment. Forms encapsulin nanocompartments about 24 nm in diameter from 60 monomers, probably involved in sulfur metabolism (By similarity). Probably encapsulates cysteine desulfurase (Probable). Sequence Mass (Da): 33671 Sequence Length: 307 Domain: Has 4 domains; an N-terminal arm not found in the type 1 subfamily, a discontinuous peripheral domain (P), an elongated loop (E) and the discontinuous axial domain (A). Subcellular Location: Encapsulin nanocompartment
Q55032
MTDNAPQLALRDVAARQLANATKTVPQLRTITPRWLVRLLHWTPVEAGIYRVNQVKDASQITVACSERDESELPETFVDYIDNPREYLLSAVNTVVDVHTRISDLYSNPHDQIREQLRLTIEIMKERQESELINSREYGLLNNVAPGQLVHTRNGAPTPDDLDELLIRVWKEPAFFLAHPQAIAAFGRECTRRGVPPATVSLFGSSFITWRGVPLIPSDKVPLENGKTKILLLRVGESRQGVVGLYQPNLPGEQGMGLSVRFMGINRKALASYLVSLYCSLAVLTDDALAVLDNVDVTQYHTYRYN
Function: Shell component of a type 2A encapsulin nanocompartment. Expression in E.coli generates nanocompartments with an average diameter of 25 nm. They can be disassembled by treatment with 6M guanidine hydrochloride and reassembled with cargo. The nanocompartment is probably involved in sulfur metabolism . Probably allows passage of cysteine into its interior; during growth in light the physiological pH is 8-8.4, about 30-54% of free cysteine (charge -1) would be able to pass through the shell (Probable). Sequence Mass (Da): 34360 Sequence Length: 306 Domain: Has 4 domains; an N-terminal arm not found in the type 1 subfamily, a discontinuous peripheral domain (P), an elongated loop (E) and the discontinuous axial domain (A). Subcellular Location: Encapsulin nanocompartment
B8GKX5
MVKIGVHVSIAGSIARAVERAMAIDCDTFQIFSRNPRGWTFKPLAEEDAALFQGALGTSGIGPAVVHMPYLPNLASPKEEIWRKSVEALTEELHRCSMLDVPYLVTHLGHHMGEGIGAGEGRVQQAIDAAFSQSDPGSSRVMLLLENTAGEKNSVGSRFEEIGRIRESCSDPDRIGVCMDTCHAFAAGYDLRNEVGLSRTLEAFEDGIGIEHLHVIHLNDAKADIGSHLDRHTHIGLGMIGEEGCSGILTHPTLASLPFICETPEDAVRDNAANIRAVRRLAVPRA
Cofactor: Binds 3 Zn(2+) ions. Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. Catalytic Activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. Sequence Mass (Da): 30890 Sequence Length: 286 EC: 3.1.21.2
Q5UPY4
MQSNINICTLSIFIIESMTTRIGRHINISKGFVSAPEYAKNIGCSVFQIFLGAPQQILSKARQKDELIEFGKQLIKHDLIMVVHGSYTINLCHPPGSKKFETSIKSLVKDLNATNLIGDRCLGVIIHMGKNISENKLTVDQAIDNYVTGIKTALSQTPDNTTIVLETGASQGSEVASHIDGLAQIYWCLNDAERERVYFCIDTCHIWATGYDISSPTGVKKFFKEFDKKIGVEKISCIHFNDSKTGLESKVDRHADLCYGEIGSNGLKAIAKFAKEYKIHLIMETPLDAINPETNQEISYNEEYNKVKSWLK
Cofactor: Binds 3 Zn(2+) ions. Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues (By similarity). Catalytic Activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. Sequence Mass (Da): 34857 Sequence Length: 312 EC: 3.1.21.2
Q2RJF1
MRLGAHLSIAKGLPRTAAMATSIGANTFQYFTRNPRGGAARQIPGKEIQAWREARRRADLYPIAGHLPYTVNLGAAAERQQEFTRMVLHDDTLRVAAIDGEYLISHPGHYEGERQAGLDRIIQLIEEAYLSITPPGPMLLLETMAGQGKEVGTIDDLCYILEGLGWPDRVGVCLDSAHLFAAGWDLRTPAGCQQLVQELAAKIGLDRVKAMHLNDSAAPLGSHRDRHAGIGKGELGREGIAAVVNDPFLGELPLFLETPVANYEEYGEEIALIQKLKSV
Cofactor: Binds 3 Zn(2+) ions. Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. Catalytic Activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. Sequence Mass (Da): 30409 Sequence Length: 279 EC: 3.1.21.2
P63536
MLIGSHVSPTDPLAAAEAEGADVVQIFLGNPQSWKAPKPRDDAAALKAATLPIYVHAPYLINLASANNRVRIPSRKILQETCAAAADIGAAAVIVHGGHVADDNDIDKGFQRWRKALDRLETEVPVYLENTAGGDHAMARRFDTIARLWDVIGDTGIGFCLDTCHTWAAGEALTDAVDRIKAITGRIDLVHCNDSRDEAGSGRDRHANLGSGQIDPDLLVAAVKAAGAPVICETADQGRKDDIAFLRERTGS
Cofactor: Binds 3 Zn(2+) ions. Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. Catalytic Activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. Sequence Mass (Da): 26845 Sequence Length: 252 EC: 3.1.21.2
Q7NBA9
MKSNKIKYLGCFVGATKPDFMLGMVKTVVDYGATSFMFYSGPPQSFRRTPTAQFKLDLAKAYLAKHNLGDLGDNYVVHAPYLINLANGDSTKRERSFNFFLDELKRTNELGAKYFVLHPGSALNVKDKTQALDHLATELNRAISMTKDTIICLETMADKGQQICSKFEELRYVIDQISDKSRIGVCFDTCHVHDAGYDLAKTQELIDHFDQVIGLKYLYVIHLNDSKNPMGARKDRHANIGYGKIGFENLLNFIYHKEICNKIIILETPWIDDPIRGEVPLYKEEIEMIRNKKFVEGLVNEES
Cofactor: Binds 3 Zn(2+) ions. Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. Catalytic Activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. Sequence Mass (Da): 34550 Sequence Length: 303 EC: 3.1.21.2
P47477
MPKLLGSFISFKAPNYFVQSAQDAIAIDATALMVFLGPPHSAYRVPFNKMQFSLGYELLKTKNINSNGLVVHAPYIINCASKDPLKQQNAISVLTNEIQLCNLAGAHYLVLHPGSAVAQTTNEALDNLVKVLNQVINKTKTTVICLETMAGKGNEIGRDLTELKYVIDRIVDKDRIGVCLDTCHFHDSGIDFSDLTGVFNTITTKLGFEFLKVIHLNESKNNCGSKKDRHANINAGMIGFENLMKFISHPQIKDLPIILETPSTSLNYPTIYREEISQIRSWFKTYQPDAN
Cofactor: Binds 3 Zn(2+) ions. Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. Catalytic Activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. Sequence Mass (Da): 32391 Sequence Length: 291 EC: 3.1.21.2
B8ZSC5
MLIGSHVSSTDPLAAAEVEGADVVQIFLGNPQSWKAPTLRSDADVLKATALPVYVHAPYLINVASANSRVRIPSRKILQQTCDAAADIGAAAVVVHGGYVADDNDLEDGFQRWRKALDQLQTDVPVYLENTAGGDHAMARRFDTIARLWDVIGETGIGFCLDTCHAWAAGEGLIHVVDRIKAITGRIDLVHCNDSKDEAGSGRDRHANLGSGQIDAELLVAAVKVAGAPVICETAEEGRKDDIAFLREKTSG
Cofactor: Binds 3 Zn(2+) ions. Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. Catalytic Activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. Sequence Mass (Da): 26861 Sequence Length: 252 EC: 3.1.21.2
Q6MUC7
MDKILLGCHVSMNKQNNYLVGSVNEAISYKANTFMIFTGPPQSTLRTNTNHLYINQMHELMNSYKIDAKDLVVHAPYIINIANSVDQNKWKFTVDFLIQEIKRCEEIKIPTLVLHPGSYTTGNYKDSLNQIIKALNIVSNYQVNVKIALETMSGKGTEVCSRLEDFKYILDNVKNKDKVGVCLDTCHLHDAGYDLSKWTEFKEQMKQNFSLDKVLCIHLNDSKNMISSHKDRHANIGYGYVGFDTLVNVVFDKDFSNISKILETPYIDKTPPYKIEIEDLLNKTFTNRL
Cofactor: Binds 3 Zn(2+) ions. Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. Catalytic Activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. Sequence Mass (Da): 33160 Sequence Length: 289 EC: 3.1.21.2
Q98PU8
MIKLGSHVSFKSPNYLYDSIKESIKNGANCAMIFLGAPQNTKRVDFEKYQYEKYLKDFSNLIKPEDIVVHAPYIINPASLEKADFAISFLSSEIKRMDKAKFKYLVLHPGFYGKNNVKDSLDQLARSIQKIFEITKDSNVEIMLETMSGKGSEVGKSYEEILYVIDKVKSPRLGACLDTCHVWDAGYNINDYQVFKDELIKTGILKHIKVIHLNDSKNELGSHKDRHANIDKGLIGLKNLKRIVHDPIFENIPIILETPWTEKGPIYDQEIAMLLEK
Cofactor: Binds 3 Zn(2+) ions. Function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate. Catalytic Activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. Sequence Mass (Da): 31685 Sequence Length: 277 EC: 3.1.21.2
Q9YBF1
MGDRCAPIKASGVLIGDSVLVTDVEQARSLYSCGYYGQPLDVEKPRGADFEGPLRLSLIESLYLAEKGVLEVAKPDGSSVGVEDLRTAVRGNPRFSMLYNIYRDLRERGFVVRSGLKFGSDFAVYRLGPGIDHAPFIVHAYSPEDNIDPVEIVRAGRLSHSVRKKFVFAVTRGGDVSYLMIDWFRP
Function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. Catalytic Activity: pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. Sequence Mass (Da): 20602 Sequence Length: 186 EC: 4.6.1.16
O29362
MIGGDFAVVKAKKSLERRGFGVKRGDKIYLHPLEVVYLQIKGIESFGELEDVLSWAESRMEDFSTYYFVYEDLRDRGNKVKIQGEFLLTKKPYLPISERKTIRMEEIAEKARNFDELRLAVVDEESEITYFRVYEPDMMGEQKEELPEIAGILSDEYVITKQTEIFSRYFYGSEKGDLVTLSLIESLYLLDLGKLNLLNADREELVKRAREVERNFDRRYEVYRNLKERGFVVKTGFKFGSEFRVYRKVESVDDLPHSEYLVDIADSREIRLIDLARAVRLAQNVRKRMVFAYGKNYLCFERVKV
Function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. Catalytic Activity: pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. Sequence Mass (Da): 35959 Sequence Length: 305 EC: 4.6.1.16
O07118
MQGRLEDGVVHLPGDARQRFHDSRGYGRPTGGDDLEVAPVEAAHLLSRDDIDGVDGMGLRELLARTGTTLDFVVYKDLRDRGFYLSPAREGWPGVADAADADFLVYPRGKGPWDGEVEHRVRVVGERESIPVSSLGEVVLAIVDEDGDLTYFDTEGDDPEGTAAEDLPADLDAELLSDRALVWDGVDRLYQRGFFGQRLYGRNADSGPLQLSLLEAAYLARADALAIDEADVVSRGRDVEGDRFDRRLAVYAALREAKTVPKSGFKFGSDFRVYTEFESVDDLSHSEFLVRVVAPDHTFVPRDLSLDVRLAGGVRKRMVFALTDDNGEIDWLSVSRLTP
Cofactor: Divalent cations; Ca(2+) better than Mg(2+). Function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. PTM: The N-terminus is blocked. Catalytic Activity: pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. Sequence Mass (Da): 37418 Sequence Length: 339 EC: 4.6.1.16
Q8TGX1
MKTQLEGDRVLAGKEAVAELYKTGYFGRPREDGLELSLVEAAYLQFRGKIEIELEGRKLDFRALFEQASLRQPNFELKYIVYKDLKERGYYVQPSAADFRVYPRGSHPGKSAAKIFVHVLSERQPLPVKLLQDSVISAENVHKQFILAVVDEESDLTFYEIKTASPQGEMPEPYPEVKTDATFLEDRVIAWDAEASGALYAGGFYGKMLDPERLQLSLVESLYLFSRGIIVVRDRKDRIFSFDEFVEKASEIESSFLRKYGAYKALRDSGHVVKTGFKFGTHFRVYRKVESIEKIPHSEYLVNVIPSDYEFRLPVMSGAVRLANSVRKRMLFAVEKEEGVEYLDISRVKM
Function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity). Catalytic Activity: pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. Sequence Mass (Da): 40133 Sequence Length: 350 EC: 4.6.1.16
Q58819
MVRDKMGKKITGLLDGDRVIVFDKNGISKLSARHYGNVEGNFLSLSLVEALYLINLGWLEVKYKDNKPLSFEELYEYARNVEERLCLKYLVYKDLRTRGYIVKTGLKYGADFRLYERGANIDKEHSVYLVKVFPEDSSFLLSELTGFVRVAHSVRKKLLIAIVDADGDIVYYNMTYVKP
Function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. Catalytic Activity: pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. Sequence Mass (Da): 20664 Sequence Length: 179 EC: 4.6.1.16
Q8TGZ7
MLCAGNGGKELPRAKVFEGGSLVSKDYEDLKRRYFGTEHGNVLFLDPFETVYLTEKGEIDPETPEGEPMSVEELLSFFERRRPGFRAGYVVYRDLTERGYVVKSGFKYGGRFRVYEEDPDREHSKYVVRVVEPDTELSTRDVLRATRLAHSVRKDFVLAVVEDVEEPRIEYVMWRWKRL
Function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. Catalytic Activity: pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. Sequence Mass (Da): 20939 Sequence Length: 179 EC: 4.6.1.16
O07165
MRVEGQLGDEVVTIKATSIARRLHGKSHYGKMYEDRLQLSLIEAAYLMERGKLKLMKDDDEVSPEEFISLLGERGLYSKYLVYRDLRNRGYIVKTGFKYGAEFRLYERGGAPGRTHSAYLVRVISENDTIHALDFSSYVRVAHGVNKKLLMAFLDDEEDITYYLVDWIRP
Function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. Catalytic Activity: pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. Sequence Mass (Da): 19693 Sequence Length: 170 EC: 4.6.1.16
Q7Q9C0
MAAVVALGANVLSAKSIICDIEGTTTSISFVKDTLFPYALKHVEGYLKNNWNEEATKTVVTALREQAEEDKKAEVEGVVPIPTGDSEDIIPEIVKNVEWQMSLDRKTGSLKTLQGLVWAKGYKDGSIKGHVYDDVQKAFEQWTENGRKIYIYSSGSVDAQKLLFEHSEQGDLLKYLSGHYDTKIGAKREKESYTSILKNIESSPEEALFLTDVYAEAKAAKEAGLNVVLLDRPGNSELSEEERKDFPVIATFSDLSFAAETKEENGGATNGKRKIEETNDDVAEEDKAQVYPNKK
Cofactor: Binds 1 Mg(2+) ion per subunit. Function: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Catalytic Activity: 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate Sequence Mass (Da): 32682 Sequence Length: 295 Pathway: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Subcellular Location: Cytoplasm EC: 3.1.3.77
Q753F5
MEEDYGVFILDVEGTVCPIAFVREQLFPYFLDKVEELINNADETERDLLADMQSRHGGAAAASLLRQLVAEDVKDPALKALQGRVWERGYASGEITAPVYADAVRFIQRNAGRVYIYSSGSVQAQRLLFGHVSNPSGDGVLDLTGHLAGFFDIPAAGRKTEAKSYERILAAIGLERQPGAAIFVSDSVAELDAASASGLSVRLAVRPGNERVSGARYTTLTDFEGL
Cofactor: Binds 1 Mg(2+) ion per subunit. Function: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Catalytic Activity: 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate Sequence Mass (Da): 24411 Sequence Length: 226 Pathway: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Subcellular Location: Cytoplasm EC: 3.1.3.77
Q0VD27
MVVLSVPAEVTVILLDIEGTTTPIAFVKDILFPYVKENVEEYLQAHWEEEECQQDVRLLRKQAEEDSHLDGAVPIPAASGNGADDPQWMIQAVVDNVYWQMSLDRKTTALKQLQGHMWRAAFKAGHMKAEFFEDVVPAVRKWREAGMKVYVYSSGSVEAQKLLFGHSTEGDILELVDGHFDTKIGHKVESESYQKIASSIGCSTNNILFLTDVSREASAAEEAGVHVAVVVRPGNAGLTDDEKTHFSLITSFSELYLPSST
Cofactor: Binds 1 Mg(2+) ion per subunit. Function: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Catalytic Activity: 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate Sequence Mass (Da): 28930 Sequence Length: 261 Pathway: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Subcellular Location: Cytoplasm EC: 3.1.3.77
C3XR70
MSDSRLRRRQGTAGTDNKRRADGPHDISGLLDGVSVVLLDIEGTTTPITFVKDELFPYVCSHVRQHLEEHWKEEECQEDIAALRKQAKEDKEMDGVVLIPECTTKDDDEEARKKVLSAVVDNVLLNMDADRKVTALKQLQGHMWRAAYQTGKIKGEYVKLTFADVVPAIRGWLETGRQVYIYSSGSVEAQKLLFGFSTEGDLLELFSGHFDTTTGLKVETESYRRIAKTVGCDPANILFLTDVVREAKPSREAGMKTCLTVRPGNAPLTEEDWANYPVIKSFSELACDVSPTKMRSRGKAAT
Cofactor: Binds 1 Mg(2+) ion per subunit. Function: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Catalytic Activity: 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate Sequence Mass (Da): 33701 Sequence Length: 302 Pathway: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Subcellular Location: Cytoplasm EC: 3.1.3.77
A8Y461
MTNTTTIQFNALLLDIEGTITSISFVKDELFPYAFENVGKYLEEHYDKPATQIIIEDLRRLAEQQLETDADVVKIRERKQECIEDVTKNVRHWIKRDKKLTPMKALQGLIWEEAYQRGYVKGHVYPDVLPILKIIESRQIPIYIYSSGSVHAQKLLFANSVEGDMTKILYGYFDTNIGLKGETSSYTKISEQIGVPEKDILFLTDVEAEAAAASKAGLQTRLVIRPGNATLTQEAKNAYGTIHTLEEIL
Cofactor: Binds 1 Mg(2+) ion per subunit. Function: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Catalytic Activity: 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate Sequence Mass (Da): 28242 Sequence Length: 249 Pathway: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Subcellular Location: Cytoplasm EC: 3.1.3.77
Q21012
MTTTTIQFNALLLDIEGTITSISFVKDELFPYAFENVGNYLEEHYDNPATQIIVEDLRHIADQQAENDVAVVRIREPRKECIEDVTKNVRHWIKRDKKLTPMKALQGLIWEEAYQRGDVKGHVYPDVLPVLKIVENRKIPIYIYSSGSVHAQKLLFANSIEGDMTKILYGYFDTNIGLKGESNSYTKISERIKIPPSEILFLTDVEAEAAAAKKAGLQTKLVVRPGNAGLTQEAINAYGTIESLEEIL
Cofactor: Binds 1 Mg(2+) ion per subunit. Function: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Catalytic Activity: 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate Sequence Mass (Da): 27955 Sequence Length: 248 Pathway: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Subcellular Location: Cytoplasm EC: 3.1.3.77
Q5AM80
MTTTAKTDDIPIDTVILDIEGTVCPITFVKDTLFPYFIEKLPSILDKFQYPLSNTSASSDDQVLNILKQLPDNITKSSESIYKHFKNLVDQDIKDPILKSLQGLIWKQGYEKNELQAPIYQDSIEFIESFPTKSSTNNKIYIYSSGSIKAQILLFGHVKSTTTTITNEVIDLNPKLNGYFDITTAGFKNQSNSYKKILQEINKSSTPKSVLFLSDNINEVNAAIEAGMKSYIVIRPGNPPIDDDDDGNDDKINHKIIYSLDELDL
Cofactor: Binds 1 Mg(2+) ion per subunit. Function: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Catalytic Activity: 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate Sequence Mass (Da): 29786 Sequence Length: 265 Pathway: Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Subcellular Location: Cytoplasm EC: 3.1.3.77