README.md

---
pretty_name: "GAMBA Functional Region Multiclass"
license: mit
tags:
- biology
- genomics
- dna
- genome-language-model
- benchmark
- functional-genomics
- multiclass-classification
- hg38
- parquet
configs:
- config_name: full-causal
  data_files:
  - split: all
    path: functional-multiclass-gamba-full-causal.parquet
- config_name: full-bidi
  data_files:
  - split: all
    path: functional-multiclass-gamba-full-bidi.parquet
- config_name: 100bp-causal
  data_files:
  - split: all
    path: functional-multiclass-gamba-100bp-causal.parquet
- config_name: 100bp-bidi
  data_files:
  - split: all
    path: functional-multiclass-gamba-100bp-bidi.parquet
---

# GAMBA Functional Region Multiclass

This dataset packages GAMBA's multiclass functional-region benchmark. Each row
is an annotated feature labeled by its functional category.

The full-region and 100 bp pooling variants are separate Hugging Face configs.
Each pooling variant has causal and bidirectional context configs.
`pool_start` and `pool_end` select either the complete ROI or a deterministic
100 bp subspan. ROIs shorter than 100 bp are absent from the 100 bp configs.

GAMBA's evaluator selects the 100 bp subspan using Python's process-randomized
`hash()`. This release uses BLAKE2 over the same seed, category, and pair ID so
the saved subset is stable across machines.

## Configs

| Config | Train rows | Test rows | Total | Pooling span |
|---|---:|---:|---:|---|
| `full-{causal,bidi}` | 75,364 | 18,917 | 94,281 | complete ROI |
| `100bp-{causal,bidi}` | 54,029 | 13,595 | 67,624 | 100 bp |

Every config includes the `noncoding_regions` extension. Filter
`category != "noncoding_regions"` to recover the exact GAMBA paper datasets:
84,837 full-region rows or 58,643 100 bp rows. The superset avoids storing the
paper rows twice.

## Choosing a context

The GAMBA paper uses context geometry matched to the model. Choose
`*-causal` for Evo2 or another left-to-right model: the ROI is at the end of
the strand-oriented window, so all preceding bases are usable context. Choose
`*-bidi` for GAMBA encoders, the distilled student, GPN-Star, PhyloGPN, or
another masked/bidirectional model: the ROI is centered so the model sees both
flanks. Using a causal file for a bidirectional model removes its right-side
context and is not the paper protocol.

## Loading

```python
from datasets import load_dataset

full = load_dataset(
    "Taykhoom/functional-multiclass-gamba",
    "full-bidi",
    split="all",
)
paper = full.filter(lambda row: row["category"] != "noncoding_regions")
test = paper.filter(lambda row: row["split"] == "test")
```

Here, `split="all"` tells Hugging Face to load the single physical parquet
file. The parquet's own `split` column records the biological chromosome split
(`train` or `test`). Keeping one file avoids duplicating the dataset; filter
the column as shown above.

## Columns

| Column | Description |
|---|---|
| `split`, `label` | Probe/fine-tuning `train` or held-out `test`; `label` is the functional category. |
| `sequence` | Strand-oriented causal or bidirectional context of at most 2,048 bp. |
| `context_policy` | `causal` for `*-causal`; `symmetric` for `*-bidi`. |
| `pair_id` | Stable GAMBA feature/control identifier. |
| `category`, `scope` | Functional category and `full` or `100bp`. |
| `chrom`, `start`, `end`, `strand` | Zero-based, half-open hg38 feature coordinates. |
| `context_start`, `context_end` | Forward-genome coordinates of `sequence`. |
| `roi_start`, `roi_end` | Full feature offsets within `sequence`. |
| `pool_start`, `pool_end` | Full-ROI or fixed 100 bp pooling span. |
| `name` | Source annotation name. |
| `phylop_mean`, `phylop_std` | Mean and population standard deviation over GAMBA's baseline ROI or fixed 100 bp span. |
| `phylop_frac_pos`, `phylop_frac_neg` | Fractions of pooled bases with positive or negative phyloP scores. |
| `phylop_mean_pos`, `phylop_mean_neg` | Means over positive or negative pooled scores, or zero when absent. |
| `phylop_context_mean`, `phylop_context_std` | Mean and population standard deviation over GAMBA's symmetric 2,048 bp phyloP context. |
| `phylop_context_frac_pos`, `phylop_context_frac_neg` | Fractions of context bases with positive or negative scores. |
| `phylop_context_mean_pos`, `phylop_context_mean_neg` | Means over positive or negative context scores, or zero when absent. |

## Data source and processing

The processing code reproduces the ten-category BED output from
[Microsoft GAMBA](https://github.com/microsoft/gamba) commit `e83984e`
byte-for-byte, then freezes all GAMBA autosomes plus `chrX` in parquet.
The GAMBA models were pretrained on the chromosomes marked `train`. The paper's
zero-shot result used the frozen models on `chr2`, `chr3`, `chr16`, and
`chr22`, which are marked `test`; no task-specific model was fitted on these
benchmark rows. Use only `test` to reproduce that paper subset. The `train`
rows are provided for probing/fine-tuning or broader chromosome evaluation.

PhyloP values come from the Zoonomia 241-mammalian track used by GAMBA.
Uncovered positions are zero and scores are rounded to two decimal places
before the float32 summaries are computed.

## Citation

Please cite the GAMBA paper:

Consens, M. E. et al. *Predicting evolutionary rate as a pretraining task
improves genome language model representations*. bioRxiv (2026).
[doi:10.64898/2026.02.02.703275](https://doi.org/10.64898/2026.02.02.703275).
A conference version is available on
[OpenReview](https://openreview.net/forum?id=dkgM2ale4U).

```bibtex
@article{consens2026predicting,
  title = {Predicting evolutionary rate as a pretraining task improves genome language model representations},
  author = {Consens, Micaela Elisa and Yang, Kevin K. and Hall, Jimmy and Conard, Ashley Mae and Wang, Bo and Crawford, Lorin and Moses, Alan and Lu, Alex X.},
  journal = {bioRxiv},
  year = {2026},
  doi = {10.64898/2026.02.02.703275},
  url = {https://doi.org/10.64898/2026.02.02.703275}
}
```

## License

GAMBA and its released benchmark code/data are distributed under the MIT
license. Upstream annotation resources retain their original terms.