Update README.md

README.md

@@ -4,5 +4,22 @@ tags:
 - biology
 ---
 
-Source:
-Sanjay R. Srivatsan *et al.*, "Massively multiplex chemical transcriptomics at single-cell resolution", *Science*, **367**, 45–51 (2020). [DOI:10.1126/science.aax6234](https://doi.org/10.1126/science.aax6234)
+## Dataset Description
+
+**A549 Chemical Perturbation Dataset (SciPlex2)**
+
+This dataset captures transcriptional responses of **A549**, a human lung adenocarcinoma cell line, to drug perturbations. Cells were treated with one of four small molecules:
+
+- **Dexamethasone**, corticosteroid receptor agonist  
+- **Nutlin-3a**, p53-MDM2 interaction antagonist  
+- **BMS-345541**, inhibitor of NF-κB–dependent transcription  
+- **Vorinostat (SAHA)**, histone deacetylase (HDAC) inhibitor  
+
+Each compound was applied for **24 hours** across **seven doses**, in **triplicate**, resulting in **84 compound–dose–replicate conditions**, along with additional vehicle controls. Single-cell transcriptional profiles were generated using **sci-RNA-seq**.
+
+## Citation
+
+> Srivatsan, S. R., McFaline-Figueroa, J. L., Ramani, V. *et al.*  
+> **Massively multiplex chemical transcriptomics at single-cell resolution**  
+> *Science*, **367**, 45–51 (2020).  
+> [https://doi.org/10.1126/science.aax6234](https://doi.org/10.1126/science.aax6234)