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3c31eef | 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 | # Z-Screen Dataset: Global Data Map & Architecture Summary
This report maps the structural dimensions, screening configurations, cell lines, libraries, and device associations for the current dataset.
> [!NOTE]
> This dataset contains transcriptomic counts representing **768,162 nanowells** profiled across **49 devices** (0–48), testing **14 libraries** against **6 cell lines / clone variants**.
>
> **Nanowell Architecture:** Each row/observation in the data represents a single **nanowell** rather than an individual cell. Each physical nanowell is **90 microns in diameter by 120 microns tall** and holds approximately **2–10 cells**.
---
## 📊 High-Level Metrics
| Metric | Count | Details |
| :--- | :--- | :--- |
| **Total Wells (Observations)** | **768,162** | Mapped 1-to-1 across `MasterFile`, `chemistry`, and `counts.h5ad` |
| **Total Features (Genes)** | **46,944** | Gene expression profiles stored in `counts.h5ad` |
| **Screening Devices** | **49** | Numbered 0 to 48 |
| **Unique Cell Lines** | **6** | HEK293, HEK293-clone, A549, H1650, THP1, AEC7 |
| **Screened Libraries** | **14** | 9 Named control compounds/control libraries, 3 OBOC libraries, 2 Control bead libraries |
| **Unique Compounds / Precursors** | **142,227** | 78 named controls, 142,149 OBOC building blocks, and controls |
---
## 🧪 Libraries & Nomenclature Breakdown
The screening libraries and control sets are classified using the following nomenclature:
* **`ZEL` Prefix**: Represents **One-Bead One-Compound (OBOC) combinatorial libraries** (testing building block precursor fragments, e.g., `ZEL024`, `ZEL028-2`, `ZEL031`).
* **`ZIC` Prefix**: Represents **named control compounds** (testing targeted, fully synthesized compounds, e.g., target inhibition screen `ZIC008`).
* **No-Compound Controls**:
* **`ZEL026-2`** represents a **set of no-compound control beads** (not a combinatorial library).
* **`ZIC005`** & **`ZIC005-2`** represent **no-compound controls** (with `ZIC005` profiling water/vehicle controls).
| Library | Nomenclature Category | Unique Compound IDs | Unique SMILES | Unique Names | Nanowell Count |
| :--- | :--- | :---: | :---: | :---: | :---: |
| **ZIC008** | Named Control Compounds | 35 | 35 | 35 | 256,052 |
| **ZEL024** | OBOC Combinatorial Library | 13,934 | 13,934 | 1 | 175,285 |
| **ZEL028-2** | OBOC Combinatorial Library | 117,950 | 117,950 | 1 | 134,821 |
| **ZEL026-2** | No-Compound Control Beads | 0 | 0 | 1 | 95,583 |
| **ZEL031** | OBOC Combinatorial Library | 10,265 | 10,265 | 1 | 43,241 |
| **ZIC009** | Named Control Compounds | 31 | 31 | 31 | 31,107 |
| **ZIC002** | Named Control Compounds | 7 | 0 | 7 | 10,999 |
| **ZIC005-2** | No-Compound Control | 0 | 0 | 1 | 7,891 |
| **ZIC001** | Named Control Compounds | 2 | 2 | 2 | 6,569 |
| **ZIC006** | Named Control Compounds | 1 | 1 | 1 | 2,099 |
| **ZIC004** | Named Control Compounds | 1 | 1 | 1 | 1,913 |
| **ZIC003** | Named Control Compounds | 2 | 0 | 2 | 1,880 |
| **ZIC007** | Named Control Compounds | 4 | 4 | 4 | 565 |
| **ZIC005** | No-Compound Control (Water) | 1 | 1 | 1 | 157 |
| **Total** | | **142,227** | **142,218** | **79** | **768,162** |
---
## 🧫 Cell Line Screen Matrix
The table below shows the nanowell count distribution for each library screened across each cell line:
| Library | A549 | AEC7 | H1650 | HEK293 | HEK293-clone | THP1 | Total Wells |
| :--- | :---: | :---: | :---: | :---: | :---: | :---: | :---: |
| **ZEL024** | 0 | 0 | 21,545 | 153,740 | 0 | 0 | 175,285 |
| **ZEL026-2** | 26,718 | 36,827 | 17,086 | 0 | 14,952 | 0 | 95,583 |
| **ZEL028-2** | 42,435 | 0 | 26,694 | 65,692 | 0 | 0 | 134,821 |
| **ZEL031** | 20,868 | 0 | 431 | 0 | 0 | 21,942 | 43,241 |
| **ZIC001** | 0 | 0 | 1,229 | 3,858 | 0 | 1,482 | 6,569 |
| **ZIC002** | 0 | 0 | 806 | 9,558 | 0 | 635 | 10,999 |
| **ZIC003** | 0 | 0 | 378 | 919 | 0 | 583 | 1,880 |
| **ZIC004** | 122 | 0 | 411 | 0 | 0 | 1,380 | 1,913 |
| **ZIC005** | 0 | 0 | 109 | 0 | 0 | 48 | 157 |
| **ZIC005-2** | 0 | 7,891 | 0 | 0 | 0 | 0 | 7,891 |
| **ZIC006** | 207 | 0 | 631 | 0 | 0 | 1,261 | 2,099 |
| **ZIC007** | 0 | 0 | 452 | 0 | 0 | 113 | 565 |
| **ZIC008** | 84,952 | 91,448 | 26,382 | 19,010 | 34,260 | 0 | 256,052 |
| **ZIC009** | 0 | 31,107 | 0 | 0 | 0 | 0 | 31,107 |
---
## 📱 Devices Analysis & Metadata Mapping
### 1. Is each device an individual cell line?
**Yes.** Every physical screening device (0–48) runs exactly **one cell line** (no cell line mixing on-chip).
### 2. Is each device a unique library?
**No.** Every device contains **multiple libraries** (ranging from **2 to 8** libraries pooled per device).
### 3. How many devices contain no-compound controls?
* **No-Compound Controls**: Present in **24 devices** (Devices 8, 9, 17, 18, 21, 22, 29, 30, and 33 through 48, representing `capture_only` roles and water vehicle control sets).
* **OBOC Combinatorial Libraries**: Present in **24 devices** (Devices 0–7, 10, 12–16, 19, 20, 23–28, 31, 32).
* **Only 1 Device (Device 11)** contains named control compounds exclusively with no-compound or combinatorial controls.
---
## 🗺️ Detailed Device Map
The device-by-device configuration shows cell line mapping and library counts:
| Device ID | Cell Line | Libraries Mapped | Wells Count | Control Roles |
| :---: | :--- | :--- | :---: | :--- |
| **Device 0** | HEK293 | `ZEL024, ZIC002, ZIC001` | 27,361 | OBOC Library (25,310), Named Controls (2,051) |
| **Device 1** | HEK293 | `ZEL024, ZIC002, ZIC001` | 22,011 | OBOC Library (20,398), Named Controls (1,613) |
| **Device 2** | HEK293 | `ZEL024, ZIC002, ZIC001` | 32,906 | OBOC Library (30,307), Named Controls (2,599) |
| **Device 3** | HEK293 | `ZEL024, ZIC002, ZIC001` | 30,518 | OBOC Library (28,064), Named Controls (2,454) |
| **Device 4** | HEK293 | `ZEL024, ZIC003, ZIC002, ZIC001` | 30,013 | OBOC Library (26,973), Named Controls (3,040) |
| **Device 5** | HEK293 | `ZEL024, ZIC003, ZIC002, ZIC001` | 25,266 | OBOC Library (22,688), Named Controls (2,578) |
| **Device 6** | H1650 | `ZEL024, ZIC002, ZIC005, ZIC001, ZIC003` | 20,099 | OBOC Library (19,253), Named Controls (846 - including ZIC005 Water Control) |
| **Device 7** | H1650 | `ZEL024, ZIC003, ZIC005, ZIC002, ZIC001` | 2,555 | OBOC Library (2,292), Named Controls (263 - including ZIC005 Water Control) |
| **Device 8** | H1650 | `ZEL026-2, ZIC007, ZIC004, ZIC006, ZIC003, ZIC002, ZIC001` | 12,422 | No-Compound Control Beads (10,369), Named Controls (2,053) |
| **Device 9** | H1650 | `ZEL026-2, ZIC006, ZIC003, ZIC004, ZIC002, ZIC001, ZIC007` | 7,571 | No-Compound Control Beads (6,717), Named Controls (854) |
| **Device 10** | THP1 | `ZIC006, ZEL031, ZIC007, ZIC004, ZIC005, ZIC002, ZIC003, ZIC001` | 3,748 | OBOC Library (2,837), Named Controls (911 - including ZIC005 Water Control) |
| **Device 11** | A549 | `ZIC006, ZIC004` | 257 | Named Controls (257) |
| **Device 12** | THP1 | `ZEL031, ZIC004, ZIC006, ZIC003, ZIC002, ZIC001` | 23,696 | OBOC Library (19,105), Named Controls (4,591) |
| **Device 13** | A549 | `ZIC008, ZEL031` | 13,602 | Named Controls (7,771), OBOC Library (5,831) |
| **Device 14** | A549 | `ZIC008, ZEL031` | 9,878 | Named Controls (5,583), OBOC Library (4,295) |
| **Device 15** | A549 | `ZIC008, ZEL031` | 16,898 | Named Controls (11,376), OBOC Library (5,522) |
| **Device 16** | A549 | `ZIC008, ZEL031` | 12,261 | Named Controls (7,041), OBOC Library (5,220) |
| **Device 17** | A549 | `ZIC008, ZEL026-2, ZIC004` | 13,675 | Named Controls (9,757), No-Compound Control Beads (3,918) |
| **Device 18** | A549 | `ZIC008, ZEL026-2, ZIC004` | 11,332 | Named Controls (8,428), No-Compound Control Beads (2,904) |
| **Device 19** | H1650 | `ZIC008, ZEL031` | 6,390 | Named Controls (6,283), OBOC Library (107) |
| **Device 20** | H1650 | `ZIC008, ZEL031` | 14,227 | Named Controls (13,903), OBOC Library (324) |
| **Device 21** | A549 | `ZEL026-2, ZIC008` | 27,839 | Named Controls (15,125), No-Compound Control Beads (12,714) |
| **Device 22** | A549 | `ZIC008, ZEL026-2` | 18,240 | Named Controls (11,058), No-Compound Control Beads (7,182) |
| **Device 23** | A549 | `ZIC008, ZEL028-2` | 23,608 | OBOC Library (20,152), Named Controls (3,456) |
| **Device 24** | H1650 | `ZIC008, ZEL028-2` | 14,178 | OBOC Library (12,056), Named Controls (2,122) |
| **Device 25** | A549 | `ZIC008, ZEL028-2` | 27,712 | OBOC Library (22,283), Named Controls (5,429) |
| **Device 26** | H1650 | `ZIC008, ZEL028-2` | 18,712 | OBOC Library (14,638), Named Controls (4,074) |
| **Device 27** | HEK293 | `ZIC008, ZEL028-2` | 17,999 | OBOC Library (14,494), Named Controls (3,505) |
| **Device 28** | HEK293 | `ZIC008, ZEL028-2` | 15,808 | OBOC Library (12,865), Named Controls (2,943) |
| **Device 29** | HEK293-clone | `ZIC008, ZEL026-2` | 27,829 | Named Controls (19,951), No-Compound Control Beads (7,878) |
| **Device 30** | HEK293-clone | `ZIC008, ZEL026-2` | 21,383 | Named Controls (14,309), No-Compound Control Beads (7,074) |
| **Device 31** | HEK293 | `ZIC008, ZEL028-2` | 27,521 | OBOC Library (20,770), Named Controls (6,751) |
| **Device 32** | HEK293 | `ZIC008, ZEL028-2` | 23,374 | OBOC Library (17,563), Named Controls (5,811) |
| **Device 33** | AEC7 | `ZIC008, ZEL026-2, ZIC009` | 11,498 | Named Controls (7,910), No-Compound Control Beads (3,588) |
| **Device 34** | AEC7 | `ZEL026-2, ZIC008, ZIC009` | 11,250 | Named Controls (7,340), No-Compound Control Beads (3,910) |
| **Device 35** | AEC7 | `ZIC008, ZEL026-2, ZIC009` | 9,165 | Named Controls (6,075), No-Compound Control Beads (3,090) |
| **Device 36** | AEC7 | `ZIC008, ZEL026-2, ZIC009` | 12,111 | Named Controls (8,004), No-Compound Control Beads (4,107) |
| **Device 37** | AEC7 | `ZIC008, ZEL026-2, ZIC009` | 13,390 | Named Controls (9,358), No-Compound Control Beads (4,032) |
| **Device 38** | AEC7 | `ZEL026-2, ZIC008, ZIC009` | 17,504 | Named Controls (12,360), No-Compound Control Beads (5,144) |
| **Device 39** | AEC7 | `ZIC008, ZEL026-2, ZIC009` | 21,192 | Named Controls (13,801), No-Compound Control Beads (7,391) |
| **Device 40** | AEC7 | `ZIC008, ZEL026-2, ZIC009` | 16,318 | Named Controls (10,753), No-Compound Control Beads (5,565) |
| **Device 41** | AEC7 | `ZIC008, ZIC005-2, ZIC009` | 2,929 | Named Controls (2,587), No-Compound Control (342) |
| **Device 42** | AEC7 | `ZIC008, ZIC009, ZIC005-2` | 4,660 | Named Controls (3,990), No-Compound Control (670) |
| **Device 43** | AEC7 | `ZIC008, ZIC005-2, ZIC009` | 11,537 | Named Controls (9,698), No-Compound Control (1,839) |
| **Device 44** | AEC7 | `ZIC008, ZIC005-2, ZIC009` | 10,472 | Named Controls (8,618), No-Compound Control (1,854) |
| **Device 45** | AEC7 | `ZIC008, ZIC005-2, ZIC009` | 7,223 | Named Controls (6,209), No-Compound Control (1,014) |
| **Device 46** | AEC7 | `ZIC008, ZIC005-2, ZIC009` | 8,258 | Named Controls (7,242), No-Compound Control (1,016) |
| **Device 47** | AEC7 | `ZIC008, ZIC009, ZIC005-2` | 5,983 | Named Controls (5,136), No-Compound Control (847) |
| **Device 48** | AEC7 | `ZIC008, ZIC005-2, ZIC009` | 3,783 | Named Controls (3,474), No-Compound Control (309) |
|