active_seq_same_well/README.md

ActiveSeq Same-Well Calibration Layer

This folder contains same-well RNA and imaging calibration data. It is the public layer for claims that RNA and morphology were measured from the same physical nanowell.

Files

• joined_same_well_data_with_public_compound_id.parquet
• joined_same_well_data.parquet
• assay_well_dataframe.parquet
• matches_table.parquet
• imaging_lats.parquet
• rnaseq_lats.parquet
• rnaseq_obs.parquet
• activeseq_control_name_public_compound_map.parquet

Contents

The current audit found 18,727 joined rows before collapse and 14,537 collapsed nanowells after aggregating imaging patches to physical wells. The public compound mapping covers 35 named compounds across two ActiveSeq devices.

Major columns include well identifiers, experiment/device identifiers, public compound mapping, RNA QC metrics, RNA latent features, imaging latent features, and well/patch geometry.

How To Use

This layer supports:

• Same-well RNA-image coupling analyses.
• Compound-geometry comparison between RNA and image spaces.
• Tests of whether exact well pairing adds signal beyond compound identity.
• Calibration of future imaging encoders against RNA measurements.