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PMC10000584
Bhuvana Plakkot,Ashley Di Agostino,Madhan Subramanian
Implications of Hypothalamic Neural Stem Cells on Aging and Obesity-Associated Cardiovascular Diseases
28-02-2023
aging,cardiovascular conditions,hypothalamus,neural stem cells,neuroinflammation,obesity
The hypothalamus, one of the major regulatory centers in the brain, controls various homeostatic processes, and hypothalamic neural stem cells (htNSCs) have been observed to interfere with hypothalamic mechanisms regulating aging. NSCs play a pivotal role in the repair and regeneration of brain cells during neurodegene...
Implications of Hypothalamic Neural Stem Cells on Aging and Obesity-Associated Cardiovascular Diseases The hypothalamus, one of the major regulatory centers in the brain, controls various homeostatic processes, and hypothalamic neural stem cells (htNSCs) have been observed to interfere with hypothalamic mechanisms reg...
PMC10000590
Lesly J. Bueno-Urquiza,Marcela G. Martínez-Barajas,Carlos E. Villegas-Mercado,Jonathan R. García-Bernal,Ana L. Pereira-Suárez,Maribel Aguilar-Medina,Mercedes Bermúdez
The Two Faces of Immune-Related lncRNAs in Head and Neck Squamous Cell Carcinoma
24-02-2023
HNSCC,tumor microenvironment,LncRNAs,cancer-associated fibroblasts
Head and neck squamous cell carcinoma (HNSCC) is a group of cancers originating from the mucosal epithelium in the oral cavity, larynx, oropharynx, nasopharynx, and hypopharynx. Molecular factors can be key in the diagnosis, prognosis, and treatment of HNSCC patients. Long non-coding RNAs (lncRNAs) are molecular regula...
The Two Faces of Immune-Related lncRNAs in Head and Neck Squamous Cell Carcinoma Head and neck squamous cell carcinoma (HNSCC) is a group of cancers originating from the mucosal epithelium in the oral cavity, larynx, oropharynx, nasopharynx, and hypopharynx. Molecular factors can be key in the diagnosis, prognosis, an...
PMC10000595
Alessia Garufi,Valerio D’Orazi,Giuseppa Pistritto,Mara Cirone,Gabriella D’Orazi
HIPK2 in Angiogenesis: A Promising Biomarker in Cancer Progression and in Angiogenic Diseases
02-03-2023
VEGF,HIF-1,hypoxia,micro-RNA,circular HIPK2,p53,cancer,diabetes,diabetic retinopathy,wound healing
Simple Summary Dysregulated angiogenesis contributes to cancer progression and to many chronic inflammatory diseases. Many efforts in the field of angiogenesis have been made to discover new potential molecular targets to be used as biomarkers or to improve the anti-angiogenic therapies. HIPK2, an oncosuppressor able t...
HIPK2 in Angiogenesis: A Promising Biomarker in Cancer Progression and in Angiogenic Diseases Dysregulated angiogenesis contributes to cancer progression and to many chronic inflammatory diseases. Many efforts in the field of angiogenesis have been made to discover new potential molecular targets to be used as biomark...
PMC10000606
Prajish Iyer,Lili Wang
Emerging Therapies in CLL in the Era of Precision Medicine
03-03-2023
CLL,emerging therapies,metabolism,splicing,whole-exome,transcriptome
Simple Summary Despite being a slow-proliferating disease, chronic lymphocytic leukemia (CLL) is an incurable and frequently reoccurring adult leukemia. Although large-scale genome-wide next-generation sequencing studies have provided insights into CLL’s transcriptome and mutational landscape, the molecular mechanisms ...
Emerging Therapies in CLL in the Era of Precision Medicine Despite being a slow-proliferating disease, chronic lymphocytic leukemia (CLL) is an incurable and frequently reoccurring adult leukemia. Although large-scale genome-wide next-generation sequencing studies have provided insights into CLL’s transcriptome and mu...
PMC10000620
Ioana-Miruna Stanciu,Andreea Ioana Parosanu,Cristina Orlov-Slavu,Ion Cristian Iaciu,Ana Maria Popa,Cristina Mihaela Olaru,Cristina Florina Pirlog,Radu Constantin Vrabie,Cornelia Nitipir
Mechanisms of Resistance to CDK4/6 Inhibitors and Predictive Biomarkers of Response in HR+/HER2-Metastatic Breast Cancer—A Review of the Literature
05-03-2023
CDK4/6 inhibitors,advanced/metastatic breast cancer,biomarkers of response,progression on CDK4/6 inhibitors,resistance mechanisms,endocrine therapy
The latest and newest discoveries for advanced and metastatic hormone receptor-positive (HR+) and human epidermal growth factor receptor 2-negative (HER2-) breast cancer are the three cyclin-dependent kinases 4 and 6 inhibitors (CDK4/6i) in association with endocrine therapy (ET). However, even if this treatment revolu...
Mechanisms of Resistance to CDK4/6 Inhibitors and Predictive Biomarkers of Response in HR+/HER2-Metastatic Breast Cancer—A Review of the Literature The latest and newest discoveries for advanced and metastatic hormone receptor-positive (HR+) and human epidermal growth factor receptor 2-negative (HER2-) breast cancer a...
PMC10000625
Kathryn J. Brayer,Huining Kang,Adel K. El-Naggar,Simon Andreasen,Preben Homøe,Katalin Kiss,Lauge Mikkelsen,Steffen Heegaard,Daniel Pelaez,Acadia Moeyersoms,David T. Tse,Yan Guo,David Y. Lee,Scott A. Ness
Dominant Gene Expression Profiles Define Adenoid Cystic Carcinoma (ACC) from Different Tissues: Validation of a Gene Signature Classifier for Poor Survival in Salivary Gland ACC
22-02-2023
oral cancer,biomarker,MYB oncogene,transcriptome analysis,bioinformatics,survival analysis
Simple Summary Adenoid cystic carcinoma (ACC) is a pathologically distinctive tumor that most often occurs in major or minor salivary glands, but can also occur in other tissues. We compared the gene expression profiles of ACC tumor samples from salivary gland, lacrimal gland, breast or skin. Despite their different ti...
Dominant Gene Expression Profiles Define Adenoid Cystic Carcinoma (ACC) from Different Tissues: Validation of a Gene Signature Classifier for Poor Survival in Salivary Gland ACC Adenoid cystic carcinoma (ACC) is a pathologically distinctive tumor that most often occurs in major or minor salivary glands, but can also o...
PMC10000627
Irène Tatischeff
Extracellular Vesicle-DNA: The Next Liquid Biopsy Biomarker for Early Cancer Diagnosis?
24-02-2023
extracellular vesicles (EVs),exosomes (EXs),liquid biopsy (LB),EV-associated DNA (EV-DNA),cancer diagnosis
Simple Summary Each human cancer is a specific disease, but all cancers share the necessity of an early diagnosis for providing the optimal outcome for the patient. Liquid biopsy in blood, as a substitute to invasive tissue biopsies, brought the first important breakthrough for cancer diagnosis. The race for efficient ...
Extracellular Vesicle-DNA: The Next Liquid Biopsy Biomarker for Early Cancer Diagnosis? Each human cancer is a specific disease, but all cancers share the necessity of an early diagnosis for providing the optimal outcome for the patient. Liquid biopsy in blood, as a substitute to invasive tissue biopsies, brought the ...
PMC10000641
Verena Damiani,Erika Pizzinato,Ilaria Cicalini,Gianmaria Demattia,Mirco Zucchelli,Luca Natale,Claudia Palmarini,Claudia Di Marzio,Luca Federici,Vincenzo De Laurenzi,Damiana Pieragostino
Development of a Method for Detection of SARS-CoV-2 Nucleocapsid Antibodies on Dried Blood Spot by DELFIA Immunoassay
27-02-2023
SARS-CoV-2,nucleocapsid antibodies,ELISA,DELFIA,dried blood spot
Antibodies against the SARS-CoV-2 nucleocapsid protein are produced by the immune system in response to SARS-CoV-2 infection, but most available vaccines developed to fight the pandemic spread target the SARS-CoV-2 spike protein. The aim of this study was to improve the detection of antibodies against the SARS-CoV-2 nu...
Development of a Method for Detection of SARS-CoV-2 Nucleocapsid Antibodies on Dried Blood Spot by DELFIA Immunoassay Antibodies against the SARS-CoV-2 nucleocapsid protein are produced by the immune system in response to SARS-CoV-2 infection, but most available vaccines developed to fight the pandemic spread target t...
PMC10000648
Manjula P. Mony,Kelly A. Harmon,Ryan Hess,Amir H. Dorafshar,Sasha H. Shafikhani
An Updated Review of Hypertrophic Scarring
21-02-2023
normal (acute) wound healing,hypertrophic scar,keloids,animal models,treatments
Hypertrophic scarring (HTS) is an aberrant form of wound healing that is associated with excessive deposition of extracellular matrix and connective tissue at the site of injury. In this review article, we provide an overview of normal (acute) wound healing phases (hemostasis, inflammation, proliferation, and remodelin...
An Updated Review of Hypertrophic Scarring Hypertrophic scarring (HTS) is an aberrant form of wound healing that is associated with excessive deposition of extracellular matrix and connective tissue at the site of injury. In this review article, we provide an overview of normal (acute) wound healing phases (hemostasis...
PMC10000650
Márcia C. Coelho,Francisco Xavier Malcata,Célia C. G. Silva
Distinct Bacterial Communities in São Jorge Cheese with Protected Designation of Origin (PDO)
26-02-2023
cheese,microbiota,lactic acid bacteria,Leuconostoc,fermented foods,metagenomic analysis,bacterial diversity,high throughput sequencing
São Jorge cheese is an iconic product of the Azores, produced from raw cow’s milk and natural whey starter (NWS). Although it is produced according to Protected Designation of Origin (PDO) specifications, the granting of the PDO label depends crucially on sensory evaluation by trained tasters. The aim of this work was ...
Distinct Bacterial Communities in São Jorge Cheese with Protected Designation of Origin (PDO) São Jorge cheese is an iconic product of the Azores, produced from raw cow’s milk and natural whey starter (NWS). Although it is produced according to Protected Designation of Origin (PDO) specifications, the granting of the ...
PMC10000660
Micael F. M. Gonçalves,Teresa Pina-Vaz,Ângela Rita Fernandes,Isabel M. Miranda,Carlos Martins Silva,Acácio Gonçalves Rodrigues,Carmen Lisboa
Microbiota of Urine, Glans and Prostate Biopsies in Patients with Prostate Cancer Reveals a Dysbiosis in the Genitourinary System
23-02-2023
prostate cancer,microbiota,urinary tract,glans penis,urine
Simple Summary Prostate cancer (PCa) is the most common cancer diagnosed among men aged 50 years and older. There is gaining interest in the genitourinary microbiome in developing ways to control this disease. In this work, an analysis of the bacterial microbiota was performed from urine samples, glans secretions, and ...
Microbiota of Urine, Glans and Prostate Biopsies in Patients with Prostate Cancer Reveals a Dysbiosis in the Genitourinary System Prostate cancer (PCa) is the most common cancer diagnosed among men aged 50 years and older. There is gaining interest in the genitourinary microbiome in developing ways to control this dis...
PMC10000663
Athanasios Armakolas,Maria Kotsari,John Koskinas
Liquid Biopsies, Novel Approaches and Future Directions
03-03-2023
cancer,diagnosis,prognosis,CTCs,ctDNA,miRNA,proteome,exosomes,clinical applications
Simple Summary The gold standard for detecting cancer and profiling tumors is tissue biopsies. Despite this, tissue biopsies have been associated with many limitations leading to the desire for less invasive and more accurate solutions. One very attractive candidate for the diagnosis and prognosis of cancer in patients...
Liquid Biopsies, Novel Approaches and Future Directions The gold standard for detecting cancer and profiling tumors is tissue biopsies. Despite this, tissue biopsies have been associated with many limitations leading to the desire for less invasive and more accurate solutions. One very attractive candidate for the dia...
PMC10000674
Georgios-Ioannis Verras,Levan Tchabashvili,David-Dimitris Chlorogiannis,Francesk Mulita,Maria-Ioanna Argentou
Updated Clinical Evidence on the Role of Adipokines and Breast Cancer: A Review
03-03-2023
breast cancer,adipokines,adiponectine,leptin,resistin,visfatin
Simple Summary Breast cancer is currently one of the most common types of cancer and the number one cause of cancer-related deaths in women worldwide. Despite significant advances involving cancer research in cancer biology, targeted treatments, and novel surgical approaches, breast cancer poses a constant, prominent c...
Updated Clinical Evidence on the Role of Adipokines and Breast Cancer: A Review Breast cancer is currently one of the most common types of cancer and the number one cause of cancer-related deaths in women worldwide. Despite significant advances involving cancer research in cancer biology, targeted treatments, and nove...
PMC10000676
Vladimir N. Binhi
Statistical Amplification of the Effects of Weak Magnetic Fields in Cellular Translation
24-02-2023
biological effect of magnetic field,ribosome,protein translation,incorporation error,the RPM,geomagnetic field
We assume that the enzymatic processes of recognition of amino acids and their addition to the synthesized molecule in cellular translation include the formation of intermediate pairs of radicals with spin-correlated electrons. The mathematical model presented describes the changes in the probability of incorrectly syn...
Statistical Amplification of the Effects of Weak Magnetic Fields in Cellular Translation We assume that the enzymatic processes of recognition of amino acids and their addition to the synthesized molecule in cellular translation include the formation of intermediate pairs of radicals with spin-correlated electrons. Th...
PMC10000678
Konstantinos Arvanitakis,Ioannis Mitroulis,Antonios Chatzigeorgiou,Ioannis Elefsiniotis,Georgios Germanidis
The Liver Cancer Immune Microenvironment: Emerging Concepts for Myeloid Cell Profiling with Diagnostic and Therapeutic Implications
28-02-2023
The Liver Cancer Immune Microenvironment: Emerging Concepts for Myeloid Cell Profiling with Diagnostic and Therapeutic Implications Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. To date, systemic treatment for patients with unresectable or advanced disease was composed...
PMC10000680
Jihui Lee,Hara Kang
Nucleolin Regulates Pulmonary Artery Smooth Muscle Cell Proliferation under Hypoxia by Modulating miRNA Expression
06-03-2023
pulmonary artery smooth muscle cells,nucleolin,hypoxia,microRNA
Hypoxia induces the abnormal proliferation of vascular smooth muscle cells (VSMCs), resulting in the pathogenesis of various vascular diseases. RNA-binding proteins (RBPs) are involved in a wide range of biological processes, including cell proliferation and responses to hypoxia. In this study, we observed that the RBP...
Nucleolin Regulates Pulmonary Artery Smooth Muscle Cell Proliferation under Hypoxia by Modulating miRNA Expression Hypoxia induces the abnormal proliferation of vascular smooth muscle cells (VSMCs), resulting in the pathogenesis of various vascular diseases. RNA-binding proteins (RBPs) are involved in a wide range of ...
PMC10000683
Elio López-García,Antonio Benítez-Cabello,Javier Ramiro-García,Victor Ladero,Francisco Noé Arroyo-López
In Silico Evidence of the Multifunctional Features of Lactiplantibacillus pentosus LPG1, a Natural Fermenting Agent Isolated from Table Olive Biofilms
22-02-2023
probiotic,starter culture,genome overview,fermented vegetables,whole-genome sequencing
In recent years, there has been a growing interest in obtaining probiotic bacteria from plant origins. This is the case of Lactiplantibacillus pentosus LPG1, a lactic acid bacterial strain isolated from table olive biofilms with proven multifunctional features. In this work, we have sequenced and closed the complete ge...
In Silico Evidence of the Multifunctional Features of Lactiplantibacillus pentosus LPG1, a Natural Fermenting Agent Isolated from Table Olive Biofilms In recent years, there has been a growing interest in obtaining probiotic bacteria from plant origins. This is the case of Lactiplantibacillus pentosus LPG1, a lactic a...
PMC10000689
Aviral Kumar,Sosmitha Girisa,Mohammed S. Alqahtani,Mohamed Abbas,Mangala Hegde,Gautam Sethi,Ajaikumar B. Kunnumakkara
Targeting Autophagy Using Long Non-Coding RNAs (LncRNAs): New Landscapes in the Arena of Cancer Therapeutics
06-03-2023
lncRNAs,autophagy,cancer,therapeutics
Cancer has become a global health hazard accounting for 10 million deaths in the year 2020. Although different treatment approaches have increased patient overall survival, treatment for advanced stages still suffers from poor clinical outcomes. The ever-increasing prevalence of cancer has led to a reanalysis of cellul...
Targeting Autophagy Using Long Non-Coding RNAs (LncRNAs): New Landscapes in the Arena of Cancer Therapeutics Cancer has become a global health hazard accounting for 10 million deaths in the year 2020. Although different treatment approaches have increased patient overall survival, treatment for advanced stages still s...
PMC10000693
Nikita Choudhary,Robert C. Osorio,Jun Y. Oh,Manish K. Aghi
Metabolic Barriers to Glioblastoma Immunotherapy
28-02-2023
glioblastoma,immunotherapy,metabolism,tumor microenvironment,glycolysis,glutamine metabolism,lipid metabolism,tryptophan metabolism
Simple Summary Glioblastoma (GBM) is an aggressive brain tumor with limited prognosis despite multimodal treatment approaches. Various immunotherapies have been investigated to address the need for novel therapeutic options in GBM with limited success. Recently, alterations in the metabolism of cancer cells which allow...
Metabolic Barriers to Glioblastoma Immunotherapy Glioblastoma (GBM) is an aggressive brain tumor with limited prognosis despite multimodal treatment approaches. Various immunotherapies have been investigated to address the need for novel therapeutic options in GBM with limited success. Recently, alterations in the met...
PMC10000695
Cindy Barba,H. Atakan Ekiz,William Weihao Tang,Arevik Ghazaryan,Mason Hansen,Soh-Hyun Lee,Warren Peter Voth,Ryan Michael O’Connell
Interferon Gamma-Inducible NAMPT in Melanoma Cells Serves as a Mechanism of Resistance to Enhance Tumor Growth
23-02-2023
melanoma,NAMPT,interferon gamma
Simple Summary The tumor microenvironment is complex, with interacting immune and tumor cells. Immune cells release inflammatory cytokines, including interferons (IFNs), that drive tumor clearance. However, evidence suggests that tumor cells can also utilize IFNs to enhance growth and survival in certain cases. We demo...
Interferon Gamma-Inducible NAMPT in Melanoma Cells Serves as a Mechanism of Resistance to Enhance Tumor Growth The tumor microenvironment is complex, with interacting immune and tumor cells. Immune cells release inflammatory cytokines, including interferons (IFNs), that drive tumor clearance. However, evidence suggest...
PMC10000698
Hongxuan Li,Shiqian Fu,Danliangmin Song,Xue Qin,Wei Zhang,Chaoxin Man,Xinyan Yang,Yujun Jiang
Identification, Typing and Drug Resistance of Cronobacter spp. in Powdered Infant Formula and Processing Environment
03-03-2023
powdered infant formula,Cronobacter spp.,antibiotic resistance,multilocus sequence typing,transcriptomics
Cronobacter spp. is a food-borne pathogenic microorganism that can cause serious diseases such as meningitis, sepsis, and necrotizing colitis in infants and young children. Powdered infant formula (PIF) is one of the main contamination routes, in which the processing environment is an important source of pollution. In ...
Identification, Typing and Drug Resistance of Cronobacter spp. in Powdered Infant Formula and Processing Environment Cronobacter spp. is a food-borne pathogenic microorganism that can cause serious diseases such as meningitis, sepsis, and necrotizing colitis in infants and young children. Powdered infant formula (PIF)...
PMC10000706
Marcel Kemper,Carolin Krekeler,Kerstin Menck,Georg Lenz,Georg Evers,Arik Bernard Schulze,Annalen Bleckmann
Liquid Biopsies in Lung Cancer
23-02-2023
liquid biopsy,lung cancer,ctDNA,CTC,miRNA,EV
Simple Summary Liquid biopsy has recently been introduced as a novel method in cancer diagnostics. It is less invasive for patients than conventional tissue biopsies, as the assay material is drawn from peripheral blood. The detected DNA fragments, as well as the cells and extracellular vesicles, can be used as biomark...
Liquid Biopsies in Lung Cancer Liquid biopsy has recently been introduced as a novel method in cancer diagnostics. It is less invasive for patients than conventional tissue biopsies, as the assay material is drawn from peripheral blood. The detected DNA fragments, as well as the cells and extracellular vesicles, can b...
PMC10000708
Jiabin Yang,Liangtang Zeng,Ruiwan Chen,Leyi Huang,Zhuo Wu,Min Yu,Yu Zhou,Rufu Chen
Leveraging Tumor Microenvironment Infiltration in Pancreatic Cancer to Identify Gene Signatures Related to Prognosis and Immunotherapy Response
24-02-2023
pancreatic cancer,immune microenvironment,prognosis,immunotherapy,molecular subtype,F2RL1,therapeutic target
Simple Summary Pancreatic ductal adenocarcinoma (PDAC) has an insidious onset and rapid progression, and its morbidity and mortality are increasing year by year. Currently, there are limited therapeutic methods and no effective therapeutic guidance. Tumor microenvironments (TME) of PDAC are highly specific and associat...
Leveraging Tumor Microenvironment Infiltration in Pancreatic Cancer to Identify Gene Signatures Related to Prognosis and Immunotherapy Response Pancreatic ductal adenocarcinoma (PDAC) has an insidious onset and rapid progression, and its morbidity and mortality are increasing year by year. Currently, there are limited...
PMC10000713
Rosa Calvello,Chiara Porro,Dario Domenico Lofrumento,Melania Ruggiero,Maria Antonietta Panaro,Antonia Cianciulli
Decoy Receptors Regulation by Resveratrol in Lipopolysaccharide-Activated Microglia
21-02-2023
microglia,resveratrol,decoy receptors,lipopolysaccharide,cytokines
Resveratrol is a polyphenol that acts as antioxidants do, protecting the body against diseases, such as diabetes, cancer, heart disease, and neurodegenerative disorders, such as Alzheimer’s (AD) and Parkinson’s diseases (PD). In the present study, we report that the treatment of activated microglia with resveratrol aft...
Decoy Receptors Regulation by Resveratrol in Lipopolysaccharide-Activated Microglia Resveratrol is a polyphenol that acts as antioxidants do, protecting the body against diseases, such as diabetes, cancer, heart disease, and neurodegenerative disorders, such as Alzheimer’s (AD) and Parkinson’s diseases (PD). In the pr...
PMC10000725
Kristin E. Cox,Shanglei Liu,Thinzar M. Lwin,Robert M. Hoffman,Surinder K. Batra,Michael Bouvet
The Mucin Family of Proteins: Candidates as Potential Biomarkers for Colon Cancer
27-02-2023
mucins,colorectal cancer,adenocarcinoma,mucinous carcinoma,hyperplastic polyps,adenomatous polyps,adenoma,serrated polyps,prognostics
Simple Summary Colorectal cancer is the second leading cause of cancer-related deaths in the United States with an overall 5-year survival of 65%. While there have been many advances in the treatment of this disease over the past few decades, there has been minimal change in the overall five-year survival in the past t...
The Mucin Family of Proteins: Candidates as Potential Biomarkers for Colon Cancer Colorectal cancer is the second leading cause of cancer-related deaths in the United States with an overall 5-year survival of 65%. While there have been many advances in the treatment of this disease over the past few decades, there has...
PMC10000735
Iñigo Les,Mireia Martínez,Inés Pérez-Francisco,María Cabero,Lucía Teijeira,Virginia Arrazubi,Nuria Torrego,Ana Campillo-Calatayud,Iñaki Elejalde,Grazyna Kochan,David Escors
Predictive Biomarkers for Checkpoint Inhibitor Immune-Related Adverse Events
06-03-2023
immune-related adverse events,immune-checkpoint inhibitors,biomarkers,prediction,diagnosis
Simple Summary Immune-checkpoint inhibitors (ICIs) are increasingly used in the treatment of cancer, but they cause immune-related adverse events (irAEs) in around 40% of patients treated. Identifying biomarkers predictive of irAEs has become a priority for the optimal management of patients on ICIs. Herein, we review ...
Predictive Biomarkers for Checkpoint Inhibitor Immune-Related Adverse Events Immune-checkpoint inhibitors (ICIs) are increasingly used in the treatment of cancer, but they cause immune-related adverse events (irAEs) in around 40% of patients treated. Identifying biomarkers predictive of irAEs has become a priority for...
PMC10000737
Zeltzin Alejandra Ceja-Galicia,Ana Karina Aranda-Rivera,Isabel Amador-Martínez,Omar Emiliano Aparicio-Trejo,Edilia Tapia,Joyce Trujillo,Victoria Ramírez,José Pedraza-Chaverri
The Development of Dyslipidemia in Chronic Kidney Disease and Associated Cardiovascular Damage, and the Protective Effects of Curcuminoids
22-02-2023
curcumin,curcuminoids,chronic renal disease,cardiovascular disease (CVD),dyslipidemia,CKD
Chronic kidney disease (CKD) is a health problem that is constantly growing. This disease presents a diverse symptomatology that implies complex therapeutic management. One of its characteristic symptoms is dyslipidemia, which becomes a risk factor for developing cardiovascular diseases and increases the mortality of C...
The Development of Dyslipidemia in Chronic Kidney Disease and Associated Cardiovascular Damage, and the Protective Effects of Curcuminoids Chronic kidney disease (CKD) is a health problem that is constantly growing. This disease presents a diverse symptomatology that implies complex therapeutic management. One of its ...
PMC10000741
Elodie Villalonga,Christine Mosrin,Thierry Normand,Caroline Girardin,Amandine Serrano,Bojan Žunar,Michel Doudeau,Fabienne Godin,Hélène Bénédetti,Béatrice Vallée
LIM Kinases, LIMK1 and LIMK2, Are Crucial Node Actors of the Cell Fate: Molecular to Pathological Features
04-03-2023
LIMK,actin dynamics,cytoskeleton remodelling,signalling pathways
LIM kinase 1 (LIMK1) and LIM kinase 2 (LIMK2) are serine/threonine and tyrosine kinases and the only two members of the LIM kinase family. They play a crucial role in the regulation of cytoskeleton dynamics by controlling actin filaments and microtubule turnover, especially through the phosphorylation of cofilin, an ac...
LIM Kinases, LIMK1 and LIMK2, Are Crucial Node Actors of the Cell Fate: Molecular to Pathological Features LIM kinase 1 (LIMK1) and LIM kinase 2 (LIMK2) are serine/threonine and tyrosine kinases and the only two members of the LIM kinase family. They play a crucial role in the regulation of cytoskeleton dynamics by co...
PMC10000745
Dino Bekric,Daniel Neureiter,Celina Ablinger,Heidemarie Dobias,Marlena Beyreis,Markus Ritter,Martin Jakab,Johannes Bischof,Ulrich Koller,Tobias Kiesslich,Christian Mayr
Evaluation of Tazemetostat as a Therapeutically Relevant Substance in Biliary Tract Cancer
02-03-2023
biliary tract cancer,cholangiocarcinoma,EZH2 inhibitor,epigenetics,tazemetostat
Simple Summary Treating biliary tract cancer (BTC) successfully remains to be a difficult task. Standard therapeutic options encompass surgery, radiation and chemotherapy, but the median survival has not improved beyond one year. The reasons for this might be diagnosis at an already late stage and resistance towards cu...
Evaluation of Tazemetostat as a Therapeutically Relevant Substance in Biliary Tract Cancer Treating biliary tract cancer (BTC) successfully remains to be a difficult task. Standard therapeutic options encompass surgery, radiation and chemotherapy, but the median survival has not improved beyond one year. The reasons f...
PMC10000746
Ryan C. Hall,Amita M. Vaidya,William P. Schiemann,Quintin Pan,Zheng-Rong Lu
RNA-Seq Analysis of Extradomain A and Extradomain B Fibronectin as Extracellular Matrix Markers for Cancer
21-02-2023
extracellular matrix,tumor microenvironment,fibronectin,extradomain A fibronectin,extradomain B fibronectin
Alternatively spliced forms of fibronectin, called oncofetal fibronectin, are aberrantly expressed in cancer, with little to no expression in normal tissue, making them attractive biomarkers to exploit for tumor-targeted therapeutics and diagnostics. While prior studies have explored oncofetal fibronectin expression in...
RNA-Seq Analysis of Extradomain A and Extradomain B Fibronectin as Extracellular Matrix Markers for Cancer Alternatively spliced forms of fibronectin, called oncofetal fibronectin, are aberrantly expressed in cancer, with little to no expression in normal tissue, making them attractive biomarkers to exploit for tumor-...
PMC10000752
Ruth Aquino,Vidian de Concini,Marc Dhenain,Suzanne Lam,David Gosset,Laura Baquedano,Manuel G. Forero,Arnaud Menuet,Patrick Baril,Chantal Pichon
Intrahippocampal Inoculation of Aβ1–42 Peptide in Rat as a Model of Alzheimer’s Disease Identified MicroRNA-146a-5p as Blood Marker with Anti-Inflammatory Function in Astrocyte Cells
22-02-2023
microRNAs,miRNA-146a-5p,Alzheimer’s disease,Aβ1–42 peptide,biomarkers,diagnosis
Circulating microRNAs (miRNAs) have aroused a lot of interest as reliable blood diagnostic biomarkers of Alzheimer’s disease (AD). Here, we investigated the panel of expressed blood miRNAs in response to aggregated Aβ1–42 peptides infused in the hippocampus of adult rats to mimic events of the early onset of non-famili...
Intrahippocampal Inoculation of Aβ1–42 Peptide in Rat as a Model of Alzheimer’s Disease Identified MicroRNA-146a-5p as Blood Marker with Anti-Inflammatory Function in Astrocyte Cells Circulating microRNAs (miRNAs) have aroused a lot of interest as reliable blood diagnostic biomarkers of Alzheimer’s disease (AD). Here,...
PMC10000757
Maximilian Vidovic,Lars Hendrik Müschen,Svenja Brakemeier,Gerrit Machetanz,Marcel Naumann,Sergio Castro-Gomez
Current State and Future Directions in the Diagnosis of Amyotrophic Lateral Sclerosis
24-02-2023
amyotrophic lateral sclerosis,ALS,motor neuron disease,MND,diagnosis,diagnostics
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by loss of upper and lower motor neurons, resulting in progressive weakness of all voluntary muscles and eventual respiratory failure. Non-motor symptoms, such as cognitive and behavioral changes, frequently occur over the course of ...
Current State and Future Directions in the Diagnosis of Amyotrophic Lateral Sclerosis Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by loss of upper and lower motor neurons, resulting in progressive weakness of all voluntary muscles and eventual respiratory failure. Non-motor s...
PMC10000760
Mihaela Roșca,Gabriela Mihalache,Vasile Stoleru
Tomato responses to salinity stress: From morphological traits to genetic changes
10-02-2023
abiotic stress,PRISMA,salt stress,screening of salinity effects,tomato,alleviation of salinity effects
Tomato is an essential annual crop providing human food worldwide. It is estimated that by the year 2050 more than 50% of the arable land will become saline and, in this respect, in recent years, researchers have focused their attention on studying how tomato plants behave under various saline conditions. Plenty of res...
Tomato responses to salinity stress: From morphological traits to genetic changes Tomato is an essential annual crop providing human food worldwide. It is estimated that by the year 2050 more than 50% of the arable land will become saline and, in this respect, in recent years, researchers have focused their attention ...
PMC10000761
Johnny Atallah,Musie Ghebremichael,Kyle D. Timmer,Hailey M. Warren,Ella Mallinger,Ellen Wallace,Fiona R. Strouts,David H. Persing,Michael K. Mansour
Novel Host Response-Based Diagnostics to Differentiate the Etiology of Fever in Patients Presenting to the Emergency Department †
02-03-2023
host-response,diagnostics,gene signatures,fever,febrile syndromes
Fever is a common presentation to urgent-care services and is linked to multiple disease processes. To rapidly determine the etiology of fever, improved diagnostic modalities are necessary. This prospective study of 100 hospitalized febrile patients included both positive (FP) and negative (FN) subjects in terms of inf...
Novel Host Response-Based Diagnostics to Differentiate the Etiology of Fever in Patients Presenting to the Emergency Department † Fever is a common presentation to urgent-care services and is linked to multiple disease processes. To rapidly determine the etiology of fever, improved diagnostic modalities are necessary....
PMC10000766
Mona A. Amin,Halla M. Ragab,Nabila Abd El Maksoud,Wafaa Abd Elaziz
CD24 Gene Expression as a Risk Factor for Non-Alcoholic Fatty Liver Disease
04-03-2023
NAFLD,gene expression,CD24
In light of increasing NAFLD prevalence, early detection and diagnosis are needed for decision-making in clinical practice and could be helpful in the management of patients with NAFLD. The goal of this study was to evaluate the diagnostic accuracy of CD24 gene expression as a non-invasive tool to detect hepatic steato...
CD24 Gene Expression as a Risk Factor for Non-Alcoholic Fatty Liver Disease In light of increasing NAFLD prevalence, early detection and diagnosis are needed for decision-making in clinical practice and could be helpful in the management of patients with NAFLD. The goal of this study was to evaluate the diagnostic acc...
PMC10000776
Hitha Gopalan Nair,Aneta Jurkiewicz,Damian Graczyk
Inhibition of RNA Polymerase III Augments the Anti-Cancer Properties of TNFα
27-02-2023
RNA polymerase III,cancer,TNFα,NF-κB
Simple Summary Tumour necrosis factor alpha (TNFα) is a cytokine that plays an important role in apoptosis, cell survival, as well as in inflammation and immunity. Although named for its antitumor properties, TNFα has tumour-promoting properties. TNFα is often present in large quantities in tumours, and cancer cells fr...
Inhibition of RNA Polymerase III Augments the Anti-Cancer Properties of TNFα Tumour necrosis factor alpha (TNFα) is a cytokine that plays an important role in apoptosis, cell survival, as well as in inflammation and immunity. Although named for its antitumor properties, TNFα has tumour-promoting properties. TNFα is of...
PMC10000778
Pusheng Yang,Jiawei Lu,Panpan Zhang,Shu Zhang
Comprehensive Analysis of Prognosis and Immune Landscapes Based on Lipid-Metabolism- and Ferroptosis-Associated Signature in Uterine Corpus Endometrial Carcinoma
24-02-2023
lipid metabolism,ferroptosis,immunotherapy,prognostic marker,uterine corpus endometrial carcinoma
(1) Background: The effect of tumor immunotherapy is influenced by the immune microenvironment, and it is unclear how lipid metabolism and ferroptosis regulate the immune microenvironment of uterine corpus endometrial carcinoma (UCEC). (2) Methods: Genes associated with lipid metabolism and ferroptosis (LMRGs-FARs) wer...
Comprehensive Analysis of Prognosis and Immune Landscapes Based on Lipid-Metabolism- and Ferroptosis-Associated Signature in Uterine Corpus Endometrial Carcinoma (1) Background: The effect of tumor immunotherapy is influenced by the immune microenvironment, and it is unclear how lipid metabolism and ferroptosis regula...
PMC10000782
Anna Citarella,Giuseppina Catanzaro,Zein Mersini Besharat,Sofia Trocchianesi,Federica Barbagallo,Giorgio Gosti,Marco Leonetti,Annamaria Di Fiore,Lucia Coppola,Tanja Milena Autilio,Zaira Spinello,Alessandra Vacca,Enrico De Smaele,Mary Anna Venneri,Elisabetta Ferretti,Laura Masuelli,Agnese Po
Hedgehog-GLI and Notch Pathways Sustain Chemoresistance and Invasiveness in Colorectal Cancer and Their Inhibition Restores Chemotherapy Efficacy
25-02-2023
colorectal cancer,signaling pathways,chemoresistance,epithelial-to-mesenchymal transition,organoids
Simple Summary Colorectal cancer is a leading cause of cancer-related deaths, mainly caused by resistance to therapy and metastatic spread, in turn sustained by the activation of mechanisms such as the epithelial-to-mesenchymal transition (EMT). We investigate here the role of the Hedgehog-GLI and NOTCH signaling pathw...
Hedgehog-GLI and Notch Pathways Sustain Chemoresistance and Invasiveness in Colorectal Cancer and Their Inhibition Restores Chemotherapy Efficacy Colorectal cancer is a leading cause of cancer-related deaths, mainly caused by resistance to therapy and metastatic spread, in turn sustained by the activation of mechanism...
PMC10000786
Shayan Shafiee,Jaidip Jagtap,Mykhaylo Zayats,Jonathan Epperlein,Anjishnu Banerjee,Aron Geurts,Michael Flister,Sergiy Zhuk,Amit Joshi
Dynamic NIR Fluorescence Imaging and Machine Learning Framework for Stratifying High vs. Low Notch-Dll4 Expressing Host Microenvironment in Triple-Negative Breast Cancer
25-02-2023
cancer,tumor microenvironment modifier,notch-DLL4,consomic xenograft model,machine learning,binary classification,dynamic enhanced NIR imaging,indocyanine green,time series,tumor detection
Simple Summary Breast cancer is a disease that is affected by both the tumor cells and the host environment. It is well known that the tumor blood vessels are aberrant in structure and function due to rapid angiogenesis, and this aberrant vasculature plays a major role in drug delivery and therapy response of breast ca...
Dynamic NIR Fluorescence Imaging and Machine Learning Framework for Stratifying High vs. Low Notch-Dll4 Expressing Host Microenvironment in Triple-Negative Breast Cancer Breast cancer is a disease that is affected by both the tumor cells and the host environment. It is well known that the tumor blood vessels are aberr...
PMC10000814
Hussein Awada,Valeria Visconte
The Heterogeneous Complexity of Myeloid Neoplasm: Multi-Level Approaches to Study the Disease
24-02-2023
The Heterogeneous Complexity of Myeloid Neoplasm: Multi-Level Approaches to Study the Disease Myeloid neoplasms (MNs) include a spectrum of bone marrow malignancies that result from the clonal expansion and arrest of differentiation of myeloid progenitor cells [1]. MNs account for around 25% of all hematological malig...
PMC10000817
David Dubayle,Arnaud Vanden-Bossche,Tom Peixoto,Jean-Luc Morel
Hypergravity Increases Blood–Brain Barrier Permeability to Fluorescent Dextran and Antisense Oligonucleotide in Mice
24-02-2023
blood–brain barrier,permeability,centrifugation,hypergravity,dextran,antisense oligonucleotide
The earliest effect of spaceflight is an alteration in vestibular function due to microgravity. Hypergravity exposure induced by centrifugation is also able to provoke motion sickness. The blood–brain barrier (BBB) is the crucial interface between the vascular system and the brain to ensure efficient neuronal activity....
Hypergravity Increases Blood–Brain Barrier Permeability to Fluorescent Dextran and Antisense Oligonucleotide in Mice The earliest effect of spaceflight is an alteration in vestibular function due to microgravity. Hypergravity exposure induced by centrifugation is also able to provoke motion sickness. The blood–brain b...
PMC10000819
Walter Mancino,Paola Carnevali,Valeria Terzi,Pascual García Pérez,Leilei Zhang,Gianluca Giuberti,Lorenzo Morelli,Vania Patrone,Luigi Lucini
Hierarchical Effects of Lactic Fermentation and Grain Germination on the Microbial and Metabolomic Profile of Rye Doughs
27-02-2023
LAB,yeast,fermentation,rye,metagenomics,dough,metabolomics,germination
A multi-omics approach was adopted to investigate the impact of lactic acid fermentation and seed germination on the composition and physicochemical properties of rye doughs. Doughs were prepared with either native or germinated rye flour and fermented with Saccharomyces cerevisiae, combined or not with a sourdough sta...
Hierarchical Effects of Lactic Fermentation and Grain Germination on the Microbial and Metabolomic Profile of Rye Doughs A multi-omics approach was adopted to investigate the impact of lactic acid fermentation and seed germination on the composition and physicochemical properties of rye doughs. Doughs were prepared wi...
PMC10000829
Giuseppe Schepisi,Caterina Gianni,Michela Palleschi,Sara Bleve,Chiara Casadei,Cristian Lolli,Laura Ridolfi,Giovanni Martinelli,Ugo De Giorgi
The New Frontier of Immunotherapy: Chimeric Antigen Receptor T (CAR-T) Cell and Macrophage (CAR-M) Therapy against Breast Cancer
04-03-2023
chimeric antigen receptor (CAR),macrophages,T cells,immunotherapy,breast cancer
Simple Summary To date, different therapeutic strategies, including immunotherapies, have been shown to prolong survival in breast cancer patients, representing one of the most common malignancies. Our article deals with chimeric antigen receptor-based immunotherapy in breast cancer. Abstract Breast cancer represents o...
The New Frontier of Immunotherapy: Chimeric Antigen Receptor T (CAR-T) Cell and Macrophage (CAR-M) Therapy against Breast Cancer To date, different therapeutic strategies, including immunotherapies, have been shown to prolong survival in breast cancer patients, representing one of the most common malignancies. Our art...
PMC10000854
Yi Xu,Somaira Nowsheen,Min Deng
DNA Repair Deficiency Regulates Immunity Response in Cancers: Molecular Mechanism and Approaches for Combining Immunotherapy
06-03-2023
DNA damage response,cancer therapy,immunotherapy,cell death,biomarker,tumor microenvironment,DNA repair,cell death
Simple Summary DNA repair pathways play a crucial role in maintaining the stability of a cell’s genetic material. When these pathways are defective, it can lead to genomic instability in cancer cells, which can increase their ability to stimulate an immune response. Inhibiting DNA damage response, the process that help...
DNA Repair Deficiency Regulates Immunity Response in Cancers: Molecular Mechanism and Approaches for Combining Immunotherapy DNA repair pathways play a crucial role in maintaining the stability of a cell’s genetic material. When these pathways are defective, it can lead to genomic instability in cancer cells, which ca...
PMC10000856
Zheyuan Hu,Penghui Zhao,Aimei Liao,Long Pan,Jie Zhang,Yuqi Dong,Jihong Huang,Weiwei He,Xingqi Ou
Fermented Wheat Germ Alleviates Depression-like Behavior in Rats with Chronic and Unpredictable Mild Stress
22-02-2023
FWG,depression,behavior,neurotransmitters,intestinal microbes
Depression is a chronic mental illness with devastating effects on a person’s physical and mental health. Studies have reported that food fermentation with probiotics can enrich the nutritional values of food and produce functional microorganisms that can alleviate depression and anxiety. Wheat germ is an inexpensive r...
Fermented Wheat Germ Alleviates Depression-like Behavior in Rats with Chronic and Unpredictable Mild Stress Depression is a chronic mental illness with devastating effects on a person’s physical and mental health. Studies have reported that food fermentation with probiotics can enrich the nutritional values of food an...
PMC10000868
Jurate Valciukiene,Kestutis Strupas,Tomas Poskus
Tissue vs. Fecal-Derived Bacterial Dysbiosis in Precancerous Colorectal Lesions: A Systematic Review
04-03-2023
intestinal microbiota,colorectal adenoma,colorectal neoplasm,polyp,gut,carcinoma in situ,tissue-derived,fecal-derived dysbiosis,mucosa samples,stool,bacteria
Simple Summary Although alterations of intestinal bacterial microbiota have been admitted as playing one of the most important roles in colorectal carcinogenesis, the links between microbiota compositional changes and premalignant colorectal polyps have still not been fully examined. Furthermore, there is a lack of kno...
Tissue vs. Fecal-Derived Bacterial Dysbiosis in Precancerous Colorectal Lesions: A Systematic Review Although alterations of intestinal bacterial microbiota have been admitted as playing one of the most important roles in colorectal carcinogenesis, the links between microbiota compositional changes and premalignant co...
PMC10000871
Phuong-Thu Mai,Daejin Lim,EunA So,Ha Young Kim,Taner Duysak,Thanh-Quang Tran,Miryoung Song,Jae-Ho Jeong,Hyon E. Choy
Constitutive Expression of a Cytotoxic Anticancer Protein in Tumor-Colonizing Bacteria
27-02-2023
Escherichia coli,Salmonella enterica serovar Gallinarum,anticancer protein expression,host response,bacterial cancer therapy
Simple Summary This study examined the biodistribution of Escherichia coli and an attenuated strain of Salmonella enterica serovar Gallinarum with defective ppGpp synthesis after injection into tumor-bearing mice through the tail vein. Bacteria targeting tumor tissues, but not those in the liver and spleen, were metabo...
Constitutive Expression of a Cytotoxic Anticancer Protein in Tumor-Colonizing Bacteria This study examined the biodistribution of Escherichia coli and an attenuated strain of Salmonella enterica serovar Gallinarum with defective ppGpp synthesis after injection into tumor-bearing mice through the tail vein. Bacteria ta...
PMC10000876
Thimios A. Mitsiadis,Pierfrancesco Pagella,Helder Gomes Rodrigues,Alexander Tsouknidas,Liza L. Ramenzoni,Freddy Radtke,Albert Mehl,Laurent Viriot
Notch Signaling Pathway in Tooth Shape Variations throughout Evolution
27-02-2023
notch signaling,Jagged1,RNA analysis,mouse,human,tooth,dental morphology,evolution,differentiation
Evolutionary changes in vertebrates are linked to genetic alterations that often affect tooth crown shape, which is a criterion of speciation events. The Notch pathway is highly conserved between species and controls morphogenetic processes in most developing organs, including teeth. Epithelial loss of the Notch-ligand...
Notch Signaling Pathway in Tooth Shape Variations throughout Evolution Evolutionary changes in vertebrates are linked to genetic alterations that often affect tooth crown shape, which is a criterion of speciation events. The Notch pathway is highly conserved between species and controls morphogenetic processes in most...
PMC10000881
Olivia G. Huffman,Danielle B. Chau,Andreea I. Dinicu,Robert DeBernardo,Ofer Reizes
Mechanistic Insights on Hyperthermic Intraperitoneal Chemotherapy in Ovarian Cancer
22-02-2023
hyperthermia,ovarian cancer,immunity,chemotherapy,HIPEC
Simple Summary Advanced ovarian cancer is the leading cause of gynecological death with a high rate of reoccurrence indicating the critical need for improved therapeutics. Hyperthermic intraperitoneal chemotherapy (HIPEC) treatment for ovarian cancer has shown efficacy in extending patient overall survival yet the mech...
Mechanistic Insights on Hyperthermic Intraperitoneal Chemotherapy in Ovarian Cancer Advanced ovarian cancer is the leading cause of gynecological death with a high rate of reoccurrence indicating the critical need for improved therapeutics. Hyperthermic intraperitoneal chemotherapy (HIPEC) treatment for ovarian cancer...
PMC10000883
Iwona A. Ciechomska,Kamil Wojnicki,Bartosz Wojtas,Paulina Szadkowska,Katarzyna Poleszak,Beata Kaza,Kinga Jaskula,Wiktoria Dawidczyk,Ryszard Czepko,Mariusz Banach,Bartosz Czapski,Pawel Nauman,Katarzyna Kotulska,Wieslawa Grajkowska,Marcin Roszkowski,Tomasz Czernicki,Andrzej Marchel,Bozena Kaminska
Exploring Novel Therapeutic Opportunities for Glioblastoma Using Patient-Derived Cell Cultures
02-03-2023
glioblastoma,cancer stem cells,EMT,MGMT,temozolomide,doxorubicin,STAT3,EGFR inhibitor (AG1478)
Simple Summary Glioblastomas (GBM) are aggressive brain tumors with poor prognosis that need effective treatment. GBMs are characterized by extensive cellular and molecular heterogeneity which are reflected in patient-derived cell cultures, frequently used in testing potential therapeutics. Here, we established GBM-der...
Exploring Novel Therapeutic Opportunities for Glioblastoma Using Patient-Derived Cell Cultures Glioblastomas (GBM) are aggressive brain tumors with poor prognosis that need effective treatment. GBMs are characterized by extensive cellular and molecular heterogeneity which are reflected in patient-derived cell cultures...
PMC10000888
Leonie T. D. Wuerger,Felicia Kudiabor,Jimmy Alarcan,Markus Templin,Oliver Poetz,Holger Sieg,Albert Braeuning
Okadaic Acid Activates JAK/STAT Signaling to Affect Xenobiotic Metabolism in HepaRG Cells
28-02-2023
okadaic acid,HepaRG cells,CYP enzymes,NF-κB,JAK/STAT,inflammation
Okadaic acid (OA) is a marine biotoxin that is produced by algae and accumulates in filter-feeding shellfish, through which it enters the human food chain, leading to diarrheic shellfish poisoning (DSP) after ingestion. Furthermore, additional effects of OA have been observed, such as cytotoxicity. Additionally, a stro...
Okadaic Acid Activates JAK/STAT Signaling to Affect Xenobiotic Metabolism in HepaRG Cells Okadaic acid (OA) is a marine biotoxin that is produced by algae and accumulates in filter-feeding shellfish, through which it enters the human food chain, leading to diarrheic shellfish poisoning (DSP) after ingestion. Furthermo...
PMC10000895
Sabine Weiskirchen,Sarah K. Schröder,Eva Miriam Buhl,Ralf Weiskirchen
A Beginner’s Guide to Cell Culture: Practical Advice for Preventing Needless Problems
21-02-2023
contamination,mycoplasma,retrovirus,STR profiling,misidentification,cell authentication,conditional reprogramming
The cultivation of cells in a favorable artificial environment has become a versatile tool in cellular and molecular biology. Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequen...
A Beginner’s Guide to Cell Culture: Practical Advice for Preventing Needless Problems The cultivation of cells in a favorable artificial environment has become a versatile tool in cellular and molecular biology. Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, ...
PMC10000902
Huriye Ercan,Ulrike Resch,Felicia Hsu,Goran Mitulovic,Andrea Bileck,Christopher Gerner,Jae-Won Yang,Margarethe Geiger,Ingrid Miller,Maria Zellner
A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection
26-02-2023
top-down proteomics,2D-DIGE,bottom-up proteomics,shotgun proteomics,proteoforms,post-translational modification (PTM)
Proteomics is an indispensable analytical technique to study the dynamic functioning of biological systems via different proteins and their proteoforms. In recent years, bottom-up shotgun has become more popular than gel-based top-down proteomics. The current study examined the qualitative and quantitative performance ...
A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection Proteomics is an indispensable analytical technique to study the dynamic functioning of biological systems via different proteins and their proteoforms. In recent years, bottom-u...
PMC10000907
Yang Wang,Muhammad Ali,Qi Zhang,Qiannan Sun,Jun Ren,Wei Wang,Dong Tang,Daorong Wang
ATF4 Transcriptionally Activates SHH to Promote Proliferation, Invasion, and Migration of Gastric Cancer Cells
23-02-2023
transcriptional factor,ATF4,SHH,gastric cancer
Simple Summary Gastric cancer (GC) is the world’s third greatest cause of cancer-related death. Since the underlying pathogenic mechanisms are still unclear and only a limited number of specialized drugs have been developed, treating GC patients in clinical practice remains challenging. We observed that ATF4 was marked...
ATF4 Transcriptionally Activates SHH to Promote Proliferation, Invasion, and Migration of Gastric Cancer Cells Gastric cancer (GC) is the world’s third greatest cause of cancer-related death. Since the underlying pathogenic mechanisms are still unclear and only a limited number of specialized drugs have been developed...
PMC10000908
Nesrine Ben Mhenni,Giulia Alberghini,Valerio Giaccone,Alessandro Truant,Paolo Catellani
Prevalence and Antibiotic Resistance Phenotypes of Pseudomonas spp. in Fresh Fish Fillets
23-02-2023
Pseudomonas spp.,fish fillets,food microbiology,antimicrobial resistance,multi-drug resistance
In fresh fish products, excessive loads of Pseudomonas can lead to their rapid spoilage. It is wise for Food Business Operators (FBOs) to consider its presence both in whole and prepared fish products. With the current study, we aimed to quantify Pseudomonas spp. in fresh fillets of Salmo salar, Gadus morhua and Pleuro...
Prevalence and Antibiotic Resistance Phenotypes of Pseudomonas spp. in Fresh Fish Fillets In fresh fish products, excessive loads of Pseudomonas can lead to their rapid spoilage. It is wise for Food Business Operators (FBOs) to consider its presence both in whole and prepared fish products. With the current study, we ...
PMC10000909
Ehsan Gharib,Parinaz Nasri Nasrabadi,Gilles A. Robichaud
Circular RNA Expression Signatures Provide Promising Diagnostic and Therapeutic Biomarkers for Chronic Lymphocytic Leukemia
01-03-2023
cancer,chronic lymphocytic leukemia,circular RNA,diagnosis,prognosis,drug sensitivity prediction
Simple Summary This study aimed to evaluate the potential of circular RNA (circRNA) expression profiles for the early detection of chronic lymphocytic leukemia (CLL) using bioinformatic algorithms on verified CLL patient datasets. We analyzed and validated the diagnostic performance of circRNAs as potential biomarkers ...
Circular RNA Expression Signatures Provide Promising Diagnostic and Therapeutic Biomarkers for Chronic Lymphocytic Leukemia This study aimed to evaluate the potential of circular RNA (circRNA) expression profiles for the early detection of chronic lymphocytic leukemia (CLL) using bioinformatic algorithms on verified C...
PMC10000922
Eishin Morita,Hiroaki Matsuo,Kunie Kohno,Tomoharu Yokooji,Hiroyuki Yano,Takashi Endo
A Narrative Mini Review on Current Status of Hypoallergenic Wheat Development for IgE-Mediated Wheat Allergy, Wheat-Dependent Exercise-Induced Anaphylaxis
23-02-2023
deamidation,enzymic degradation,hypoallergenic wheat,ω5-gliadin,thioredoxin,wheat-dependent exercise-induced anaphylaxis
Immunoglobulin E (IgE)-mediated food allergies to wheat that develop after school age typically shows a type of wheat-dependent exercise-induced anaphylaxis (WDEIA). At present, avoidance of wheat products or postprandial rest after ingesting wheat is recommended for patients with WDEIA, depending on the severity of th...
A Narrative Mini Review on Current Status of Hypoallergenic Wheat Development for IgE-Mediated Wheat Allergy, Wheat-Dependent Exercise-Induced Anaphylaxis Immunoglobulin E (IgE)-mediated food allergies to wheat that develop after school age typically shows a type of wheat-dependent exercise-induced anaphylaxis (WDEIA)...
PMC10000926
Mengyuan Li,Angela Quintana,Elena Alberts,Miu Shing Hung,Victoire Boulat,Mercè Martí Ripoll,Anita Grigoriadis
B Cells in Breast Cancer Pathology
28-02-2023
B cells,tumour-infiltrating lymphocytes,breast cancer,tertiary lymphoid structures,lymph nodes,germinal centres
Simple Summary B cells in the tumour microenvironment and lymph nodes have affirmed their role in breast cancer pathology. Multiplex imaging, single cell, and spatial transcriptomics of cancer patients’ breast carcinomas and lymph nodes have illustrated the diversity and spatial context of B cells in this disease. Thei...
B Cells in Breast Cancer Pathology B cells in the tumour microenvironment and lymph nodes have affirmed their role in breast cancer pathology. Multiplex imaging, single cell, and spatial transcriptomics of cancer patients’ breast carcinomas and lymph nodes have illustrated the diversity and spatial context of B cells ...
PMC10000927
Benson Chellakkan Selvanesan,Alvaro de Mingo Pulido,Sheelu Varghese,Deepak Rohila,Daniel Hupalo,Yuriy Gusev,Sara Contente,Matthew D. Wilkerson,Clifton L. Dalgard,Geeta Upadhyay
NSC243928 Treatment Induces Anti-Tumor Immune Response in Mouse Mammary Tumor Models
25-02-2023
LY6K,mammary tumor model,NKT cells,B1 cells,myeloid derived suppressor cells,NSC243928,4T1,E0771
Simple Summary This study used two different syngeneic mouse mammary tumor models to determine the effect of a small molecule NSC243928 on intra-tumoral immune cells. We observed that NSC243928 treatment reduced the tumor burden in vivo and altered the wide range of immune cell infiltration in both models. These result...
NSC243928 Treatment Induces Anti-Tumor Immune Response in Mouse Mammary Tumor Models This study used two different syngeneic mouse mammary tumor models to determine the effect of a small molecule NSC243928 on intra-tumoral immune cells. We observed that NSC243928 treatment reduced the tumor burden in vivo and altered ...
PMC10000933
Artem Pilunov,Dmitrii S. Romaniuk,Anton Shmelev,Savely Sheetikov,Anna N. Gabashvili,Alexandra Khmelevskaya,Dmitry Dianov,Ksenia Zornikova,Naina T. Shakirova,Murad Vagida,Apollinariya Bogolyubova,Grigory A. Efimov
Transgenic HA-1-Specific CD8+ T-Lymphocytes Selectively Target Leukemic Cells
03-03-2023
adoptive transfer,acute myeloid leukemia,transgenic TCR,allo-HSCT,minor histocompatibility antigens,HA-1
Simple Summary A relapse of the malignant disease frequently occurs after allogeneic hematopoietic stem cell transplantation. Immune recognition of minor histocompatibility antigens, the polymorphic peptides that differ between donor and recipient, often triggers a beneficial graft-versus-leukemia response. The transge...
Transgenic HA-1-Specific CD8+ T-Lymphocytes Selectively Target Leukemic Cells A relapse of the malignant disease frequently occurs after allogeneic hematopoietic stem cell transplantation. Immune recognition of minor histocompatibility antigens, the polymorphic peptides that differ between donor and recipient, often t...
PMC10000935
Reza Zarinshenas,Arya Amini,Isa Mambetsariev,Tariq Abuali,Jeremy Fricke,Colton Ladbury,Ravi Salgia
Assessment of Barriers and Challenges to Screening, Diagnosis, and Biomarker Testing in Early-Stage Lung Cancer
03-03-2023
lung cancer,screening,biomarkers,LDCT,COVID-19
Simple Summary Lung cancer management continues to evolve with improvements in survival across all stages. The review highlights the current data supporting screening and discusses barriers to screening and potential opportunities to improve screening access. Further, the review discusses the current challenges in diag...
Assessment of Barriers and Challenges to Screening, Diagnosis, and Biomarker Testing in Early-Stage Lung Cancer Lung cancer management continues to evolve with improvements in survival across all stages. The review highlights the current data supporting screening and discusses barriers to screening and potential oppor...
PMC10000936
Henriett Butz,Éva Saskői,Lilla Krokker,Viktória Vereczki,Alán Alpár,István Likó,Erika Tóth,Erika Szőcs,Mihály Cserepes,Katalin Nagy,Imre Kacskovics,Attila Patócs
Context-Dependent Role of Glucocorticoid Receptor Alpha and Beta in Breast Cancer Cell Behaviour
01-03-2023
glucocorticoid receptor,glucocorticoid receptor alpha,glucocorticoid receptor beta,breast cancer,proliferation,migration,breast cancer progression,metastasis
Background. The dual role of GCs has been observed in breast cancer; however, due to many concomitant factors, GR action in cancer biology is still ambiguous. In this study, we aimed to unravel the context-dependent action of GR in breast cancer. Methods. GR expression was characterized in multiple cohorts: (1) 24,256 ...
Context-Dependent Role of Glucocorticoid Receptor Alpha and Beta in Breast Cancer Cell Behaviour Background. The dual role of GCs has been observed in breast cancer; however, due to many concomitant factors, GR action in cancer biology is still ambiguous. In this study, we aimed to unravel the context-dependent action...
PMC10000937
Qiyu Gan,Luning Mao,Rui Shi,Linlin Chang,Guozeng Wang,Jingxin Cheng,Rui Chen
Prognostic Value and Immune Infiltration of HPV-Related Genes in the Immune Microenvironment of Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma
23-02-2023
cervical squamous cell carcinoma and endocervical adenocarcinoma,TCGA datasets,immune microenvironment,HPV
Simple Summary Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) generally presents with HPV infection and is the second most common gynecological malignancy. However, the effect of immune infiltrate and immune microenvironment on the tumorigenesis and development of CESC remains unclear. In this ...
Prognostic Value and Immune Infiltration of HPV-Related Genes in the Immune Microenvironment of Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) generally presents with HPV infection and is the second most common gynecological mali...
PMC10000941
Alicja Rajtak,Arkadiusz Czerwonka,Michael Pitter,Jan Kotarski,Karolina Okła
Clinical Relevance of Mortalin in Ovarian Cancer Patients
23-02-2023
mortalin/mtHsp70/GRP75/PBP74/HSPA9/HSPA9B,ovarian cancer,biomarker,metastasis,recurrence,OXPHOS,EMT,stemness,RNAseq
Background: Ovarian cancer (OC) is the most lethal malignancy of the female reproductive tract. Consequently, a better understanding of the malignant features in OC is pertinent. Mortalin (mtHsp70/GRP75/PBP74/HSPA9/HSPA9B) promotes cancer development, progression, metastasis, and recurrence. Yet, there is no parallel e...
Clinical Relevance of Mortalin in Ovarian Cancer Patients Background: Ovarian cancer (OC) is the most lethal malignancy of the female reproductive tract. Consequently, a better understanding of the malignant features in OC is pertinent. Mortalin (mtHsp70/GRP75/PBP74/HSPA9/HSPA9B) promotes cancer development, progressi...
PMC10000962
Naresh Kumar Manda,Upendarrao Golla,Kishore Sesham,Parth Desai,Shrushti Joshi,Satyam Patel,Sharada Nalla,Susmitha Kondam,Lakhwinder Singh,Deepak Dewansh,Hemalatha Manda,Namita Rokana
Tuning between Nuclear Organization and Functionality in Health and Disease
23-02-2023
nuclear shape regulation,nuclear size regulation,nuclear envelope proteins,nucleophagy,nuclear lamins,nucleopathy,cancer,neurodegenerative disorders,signaling pathways,targeted therapy
The organization of eukaryotic genome in the nucleus, a double-membraned organelle separated from the cytoplasm, is highly complex and dynamic. The functional architecture of the nucleus is confined by the layers of internal and cytoplasmic elements, including chromatin organization, nuclear envelope associated proteom...
Tuning between Nuclear Organization and Functionality in Health and Disease The organization of eukaryotic genome in the nucleus, a double-membraned organelle separated from the cytoplasm, is highly complex and dynamic. The functional architecture of the nucleus is confined by the layers of internal and cytoplasmic el...
PMC10000963
Giulia Cencelli,Laura Pacini,Anastasia De Luca,Ilenia Messia,Antonietta Gentile,Yunhee Kang,Veronica Nobile,Elisabetta Tabolacci,Peng Jin,Maria Giulia Farace,Claudia Bagni
Age-Dependent Dysregulation of APP in Neuronal and Skin Cells from Fragile X Individuals
27-02-2023
Fragile X syndrome,APP processing,protein synthesis,peptide therapy,iPSCs,ADAM10,SAP97
Fragile X syndrome (FXS) is the most common form of monogenic intellectual disability and autism, caused by the absence of the functional fragile X messenger ribonucleoprotein 1 (FMRP). FXS features include increased and dysregulated protein synthesis, observed in both murine and human cells. Altered processing of the ...
Age-Dependent Dysregulation of APP in Neuronal and Skin Cells from Fragile X Individuals Fragile X syndrome (FXS) is the most common form of monogenic intellectual disability and autism, caused by the absence of the functional fragile X messenger ribonucleoprotein 1 (FMRP). FXS features include increased and dysregula...
PMC10000966
Lina Che,Caixia Zhu,Lei Huang,Hui Xu,Xinmiao Ma,Xuegang Luo,Hongpeng He,Tongcun Zhang,Nan Wang
Ginsenoside Rg2 Promotes the Proliferation and Stemness Maintenance of Porcine Mesenchymal Stem Cells through Autophagy Induction
02-03-2023
mesenchymal stem cells,cultivated meat,ginsenoside Rg2,proliferation,replicative senescence,autophagy
Mesenchymal stem cells (MSCs) can be used as a cell source for cultivated meat production due to their adipose differentiation potential, but MSCs lose their stemness and undergo replicative senescence during expansion in vitro. Autophagy is an important mechanism for senescent cells to remove toxic substances. However...
Ginsenoside Rg2 Promotes the Proliferation and Stemness Maintenance of Porcine Mesenchymal Stem Cells through Autophagy Induction Mesenchymal stem cells (MSCs) can be used as a cell source for cultivated meat production due to their adipose differentiation potential, but MSCs lose their stemness and undergo replicativ...
PMC10000969
Jazmin Rivera,Laxman Gangwani,Subodh Kumar
Mitochondria Localized microRNAs: An Unexplored miRNA Niche in Alzheimer’s Disease and Aging
25-02-2023
mitochondrial miRNAs,Alzheimer’s disease,mitochondrial dysfunction,synaptic energy,aging
Mitochondria play several vital roles in the brain cells, especially in neurons to provide synaptic energy (ATP), Ca2+ homeostasis, Reactive Oxygen Species (ROS) production, apoptosis, mitophagy, axonal transport and neurotransmission. Mitochondrial dysfunction is a well-established phenomenon in the pathophysiology of...
Mitochondria Localized microRNAs: An Unexplored miRNA Niche in Alzheimer’s Disease and Aging Mitochondria play several vital roles in the brain cells, especially in neurons to provide synaptic energy (ATP), Ca2+ homeostasis, Reactive Oxygen Species (ROS) production, apoptosis, mitophagy, axonal transport and neurotran...
PMC10000974
Evan N. Cohen,Gitanjali Jayachandran,Hui Gao,Phillip Peabody,Heather B. McBride,Franklin D. Alvarez,Megumi Kai,Juhee Song,Yu Shen,Jie S. Willey,Bora Lim,Vicente Valero,Naoto T. Ueno,James M. Reuben
Phenotypic Plasticity in Circulating Tumor Cells Is Associated with Poor Response to Therapy in Metastatic Breast Cancer Patients
06-03-2023
circulating tumor cells (CTCs),neoplastic cells,circulating,neoplasms/diagnosis,circulating/pathology,biopsy,breast neoplasms/pathology,breast cancer,biomarkers,tumor,blood,liquid biopsy,metastatic process,EMT
Simple Summary Circulating tumor cells (CTCs) have served as an independent prognostic factor in the management of metastatic breast cancer (MBC). Through the enrichment of CTCs from peripheral blood, tumor cells can be acquired multiple times during therapy and provide a broad sample of tumor heterogeneity, thereby of...
Phenotypic Plasticity in Circulating Tumor Cells Is Associated with Poor Response to Therapy in Metastatic Breast Cancer Patients Circulating tumor cells (CTCs) have served as an independent prognostic factor in the management of metastatic breast cancer (MBC). Through the enrichment of CTCs from peripheral blood, tum...
PMC10000982
Prapenpuksiri Rungsa,Htoo Tint San,Boonchoo Sritularak,Chotima Böttcher,Eakachai Prompetchara,Chatchai Chaotham,Kittisak Likhitwitayawuid
Inhibitory Effect of Isopanduratin A on Adipogenesis: A Study of Possible Mechanisms
27-02-2023
fingerroot,Boesenbergia rotunda,obesity,adipocyte,isopanduratin A,AKT/GSK3β,AMPK-ACC,MAPKs,MCE
The root of Boesenbergia rotunda, a culinary plant commonly known as fingerroot, has previously been reported to possess anti-obesity activity, with four flavonoids identified as active principles, including pinostrobin, panduratin A, cardamonin, and isopanduratin A. However, the molecular mechanisms underlying the ant...
Inhibitory Effect of Isopanduratin A on Adipogenesis: A Study of Possible Mechanisms The root of Boesenbergia rotunda, a culinary plant commonly known as fingerroot, has previously been reported to possess anti-obesity activity, with four flavonoids identified as active principles, including pinostrobin, panduratin A,...
PMC10000985
Paul T. Winnard,Laura Morsberger,Raluca Yonescu,Liqun Jiang,Ying S. Zou,Venu Raman
Isogenic Cell Lines Derived from Specific Organ Metastases Exhibit Divergent Cytogenomic Aberrations
23-02-2023
karyotypes,isogenic metastatic cell lines,inter- and intra-cytogenomic heterogeneity,comparisons
Simple Summary Normal human cells have 22 pairs of chromosomes as well as 2 sex chromosomes for a total of 46 chromosomes; this normal karyotype is called diploidy (euploidy). On the other hand, aberrant numbers of chromosomes, i.e., gains and/or losses of chromosomes, have been found in most human cancer cells. This c...
Isogenic Cell Lines Derived from Specific Organ Metastases Exhibit Divergent Cytogenomic Aberrations Normal human cells have 22 pairs of chromosomes as well as 2 sex chromosomes for a total of 46 chromosomes; this normal karyotype is called diploidy (euploidy). On the other hand, aberrant numbers of chromosomes, i.e.,...
PMC10000987
Anna Stierschneider,Benjamin Neuditschko,Katrin Colleselli,Harald Hundsberger,Franz Herzog,Christoph Wiesner
Comparative and Temporal Characterization of LPS and Blue-Light-Induced TLR4 Signal Transduction and Gene Expression in Optogenetically Manipulated Endothelial Cells
22-02-2023
endothelial cells,Toll-like receptor 4,lipopolysaccharide,optogenetic control,pro-inflammatory proteins,quantitative mass-spectrometry,chemotaxis,transmigration
In endothelial cells (ECs), stimulation of Toll-like receptor 4 (TLR4) by the endotoxin lipopolysaccharide (LPS) induces the release of diverse pro-inflammatory mediators, beneficial in controlling bacterial infections. However, their systemic secretion is a main driver of sepsis and chronic inflammatory diseases. Sinc...
Comparative and Temporal Characterization of LPS and Blue-Light-Induced TLR4 Signal Transduction and Gene Expression in Optogenetically Manipulated Endothelial Cells In endothelial cells (ECs), stimulation of Toll-like receptor 4 (TLR4) by the endotoxin lipopolysaccharide (LPS) induces the release of diverse pro-infla...
PMC10001005
Jielin Deng,Yunqiu Jiang,Zhen Bouman Chen,June-Wha Rhee,Yingfeng Deng,Zhao V. Wang
Mitochondrial Dysfunction in Cardiac Arrhythmias
21-02-2023
mitochondrial dysfunction,arrhythmia,ATP supply,reactive oxygen species
Electrophysiological and structural disruptions in cardiac arrhythmias are closely related to mitochondrial dysfunction. Mitochondria are an organelle generating ATP, thereby satisfying the energy demand of the incessant electrical activity in the heart. In arrhythmias, the homeostatic supply–demand relationship is imp...
Mitochondrial Dysfunction in Cardiac Arrhythmias Electrophysiological and structural disruptions in cardiac arrhythmias are closely related to mitochondrial dysfunction. Mitochondria are an organelle generating ATP, thereby satisfying the energy demand of the incessant electrical activity in the heart. In arrhythmias,...
PMC10001006
Federica Torricelli,Elisabetta Sauta,Veronica Manicardi,Vincenzo Dario Mandato,Andrea Palicelli,Alessia Ciarrocchi,Gloria Manzotti
An Innovative Drug Repurposing Approach to Restrain Endometrial Cancer Metastatization
03-03-2023
drug repurposing,endometrial cancer,gene expression,metastasis,PI3K pathway
Background: Endometrial cancer (EC) is the most common gynecologic tumor and the world’s fourth most common cancer in women. Most patients respond to first-line treatments and have a low risk of recurrence, but refractory patients, and those with metastatic cancer at diagnosis, remain with no treatment options. Drug re...
An Innovative Drug Repurposing Approach to Restrain Endometrial Cancer Metastatization Background: Endometrial cancer (EC) is the most common gynecologic tumor and the world’s fourth most common cancer in women. Most patients respond to first-line treatments and have a low risk of recurrence, but refractory patients, ...
PMC10001010
Fei Liu,Yidan Gao,Jian Jiao,Yuyu Zhang,Jianda Li,Luogang Ding,Lin Zhang,Zhi Chen,Xiangbin Song,Guiwen Yang,Jiang Yu,Jiaqiang Wu
Upregulation of TLR4-Dependent ATP Production Is Critical for Glaesserella parasuis LPS-Mediated Inflammation
26-02-2023
G. parasuis LPS,inflammation,ATP,TLR4,P2X7R,acute inflammatory response,pharmacological target
Glaesserella parasuis (G. parasuis), an important pathogenic bacterium, cause Glässer’s disease, and has resulted in tremendous economic losses to the global swine industry. G. parasuis infection causes typical acute systemic inflammation. However, the molecular details of how the host modulates the acute inflammatory ...
Upregulation of TLR4-Dependent ATP Production Is Critical for Glaesserella parasuis LPS-Mediated Inflammation Glaesserella parasuis (G. parasuis), an important pathogenic bacterium, cause Glässer’s disease, and has resulted in tremendous economic losses to the global swine industry. G. parasuis infection causes typica...
PMC10001020
Jiaqin Chen,Dong Feng,Yuanyuan Lu,Yanjun Zhang,Hanxiang Jiang,Man Yuan,Yifan Xu,Jianjun Zou,Yubing Zhu,Jingjing Zhang,Chun Ge,Ying Wang
A Novel Phenazine Analog, CPUL1, Suppresses Autophagic Flux and Proliferation in Hepatocellular Carcinoma: Insight from Integrated Transcriptomic and Metabolomic Analysis
05-03-2023
CPUL1,hepatocellular carcinoma,transcriptomics,metabolomics,autophagy
Simple Summary CPUL1 exhibits antitumor properties against hepatocellular carcinoma (HCC), although the underlying mechanisms remain unclear. Our study provides a comprehensive overview of the properties and molecular mechanisms of CPUL1 anti-HCC using transcriptomics and metabolomics and highlights the significance of...
A Novel Phenazine Analog, CPUL1, Suppresses Autophagic Flux and Proliferation in Hepatocellular Carcinoma: Insight from Integrated Transcriptomic and Metabolomic Analysis CPUL1 exhibits antitumor properties against hepatocellular carcinoma (HCC), although the underlying mechanisms remain unclear. Our study provides a ...
PMC10001022
Haijie Xiao,Haifeng Zhu,Oliver Bögler,Fabiola Zakia Mónica,Alexander Y. Kots,Ferid Murad,Ka Bian
Soluble Guanylate Cyclase β1 Subunit Represses Human Glioblastoma Growth
02-03-2023
glioblastoma,sGCβ1,nucleus,p53,CDK6,integrin α6,G0 arrest
Simple Summary A marked reduction in soluble guanylyl cyclase β1 (sGCβ1) transcript is characteristic for human glioma specimens. Restoring the expression of sGCβ1 inhibited the aggressive course of glioblastoma in an orthotopic xenograft mouse model. The present study is the first to reveal that sGCβ1 migrated into th...
Soluble Guanylate Cyclase β1 Subunit Represses Human Glioblastoma Growth A marked reduction in soluble guanylyl cyclase β1 (sGCβ1) transcript is characteristic for human glioma specimens. Restoring the expression of sGCβ1 inhibited the aggressive course of glioblastoma in an orthotopic xenograft mouse model. The prese...
PMC10001026
Jaesung Lee,Seohyun Chung,Minkyu Hwang,Yeongkag Kwon,Seung Hyun Han,Sung Joong Lee
Estrogen Mediates the Sexual Dimorphism of GT1b-Induced Central Pain Sensitization
06-03-2023
GT1b,pain central sensitization,estrogen,sexual dimorphism,IL-1β
We have previously reported that the intrathecal (i.t.) administration of GT1b, a ganglioside, induces spinal cord microglia activation and central pain sensitization as an endogenous agonist of Toll-like receptor 2 on microglia. In this study, we investigated the sexual dimorphism of GT1b-induced central pain sensitiz...
Estrogen Mediates the Sexual Dimorphism of GT1b-Induced Central Pain Sensitization We have previously reported that the intrathecal (i.t.) administration of GT1b, a ganglioside, induces spinal cord microglia activation and central pain sensitization as an endogenous agonist of Toll-like receptor 2 on microglia. In thi...
PMC10001027
Florian Weber,Susanne Schueler-Toprak,Christa Buechler,Olaf Ortmann,Oliver Treeck
Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling
02-03-2023
chemerin,chemokine-like receptor 1,estrogen-related receptors,ovarian cancer,overall survival,progression-free survival
Chemerin, a pleiotropic adipokine coded by the RARRES2 gene, has been reported to affect the pathophysiology of various cancer entities. To further approach the role of this adipokine in ovarian cancer (OC), intratumoral protein levels of chemerin and its receptor chemokine-like receptor 1 (CMKLR1) were examined by imm...
Chemerin and Chemokine-like Receptor 1 Expression in Ovarian Cancer Associates with Proteins Involved in Estrogen Signaling Chemerin, a pleiotropic adipokine coded by the RARRES2 gene, has been reported to affect the pathophysiology of various cancer entities. To further approach the role of this adipokine in ovarian ...
PMC10001033
Ana Sofia Coroadinha
Host Cell Restriction Factors Blocking Efficient Vector Transduction: Challenges in Lentiviral and Adeno-Associated Vector Based Gene Therapies
24-02-2023
gene therapy,lentiviral vectors,adeno-associated virus vectors,innate-immune response,host restriction factors,transduction
Gene therapy relies on the delivery of genetic material to the patient’s cells in order to provide a therapeutic treatment. Two of the currently most used and efficient delivery systems are the lentiviral (LV) and adeno-associated virus (AAV) vectors. Gene therapy vectors must successfully attach, enter uncoated, and e...
Host Cell Restriction Factors Blocking Efficient Vector Transduction: Challenges in Lentiviral and Adeno-Associated Vector Based Gene Therapies Gene therapy relies on the delivery of genetic material to the patient’s cells in order to provide a therapeutic treatment. Two of the currently most used and efficient delive...
PMC10001044
Albert Leng,Manuj Shah,Syed Ameen Ahmad,Lavienraj Premraj,Karin Wildi,Gianluigi Li Bassi,Carlos A. Pardo,Alex Choi,Sung-Min Cho
Pathogenesis Underlying Neurological Manifestations of Long COVID Syndrome and Potential Therapeutics
06-03-2023
COVID-19,SARS-CoV-2,long COVID,neurological manifestations,neurological complication,outcome,brain fog
The development of long-term symptoms of coronavirus disease 2019 (COVID-19) more than four weeks after primary infection, termed “long COVID” or post-acute sequela of COVID-19 (PASC), can implicate persistent neurological complications in up to one third of patients and present as fatigue, “brain fog”, headaches, cogn...
Pathogenesis Underlying Neurological Manifestations of Long COVID Syndrome and Potential Therapeutics The development of long-term symptoms of coronavirus disease 2019 (COVID-19) more than four weeks after primary infection, termed “long COVID” or post-acute sequela of COVID-19 (PASC), can implicate persistent neurolo...
PMC10001046
Nastaran Karimi,Seyed Javad Moghaddam
KRAS-Mutant Lung Cancer: Targeting Molecular and Immunologic Pathways, Therapeutic Advantages and Restrictions
26-02-2023
lung cancer,KRAS,molecular pathways,therapy
RAS mutations are among the most common oncogenic mutations in human cancers. Among RAS mutations, KRAS has the highest frequency and is present in almost 30% of non-small-cell lung cancer (NSCLC) patients. Lung cancer is the number one cause of mortality among cancers as a consequence of outrageous aggressiveness and ...
KRAS-Mutant Lung Cancer: Targeting Molecular and Immunologic Pathways, Therapeutic Advantages and Restrictions RAS mutations are among the most common oncogenic mutations in human cancers. Among RAS mutations, KRAS has the highest frequency and is present in almost 30% of non-small-cell lung cancer (NSCLC) patients. L...
PMC10001068
Jen-Hsiang T. Hsiao,Onur Tanglay,Anne A. Li,Aysha Y. G. Strobbe,Woojin Scott Kim,Glenda M. Halliday,YuHong Fu
Role of Oligodendrocyte Lineage Cells in Multiple System Atrophy
25-02-2023
alpha-synuclein,multiple system atrophy,neurodegeneration,oligodendrocyte,oligodendrocyte progenitor cell
Multiple system atrophy (MSA) is a debilitating movement disorder with unknown etiology. Patients present characteristic parkinsonism and/or cerebellar dysfunction in the clinical phase, resulting from progressive deterioration in the nigrostriatal and olivopontocerebellar regions. MSA patients have a prodromal phase s...
Role of Oligodendrocyte Lineage Cells in Multiple System Atrophy Multiple system atrophy (MSA) is a debilitating movement disorder with unknown etiology. Patients present characteristic parkinsonism and/or cerebellar dysfunction in the clinical phase, resulting from progressive deterioration in the nigrostriatal and o...
PMC10001073
Ziwei Liu,Hangyu Li,Qianqian Liu,Yangyang Feng,Daiyan Wu,Xinnan Zhang,Linzi Zhang,Sheng Li,Feng Tang,Qun Liu,Xiaonong Yang,Haibo Feng
Ultrasonic Treatment Enhances the Antioxidant and Immune-Stimulatory Properties of the Polysaccharide from Sinopodophyllum hexandrum Fruit
21-02-2023
ultrasonic,carbohydrate,structural characterization,antioxidant,immunomodulatory effect
We aimed to assess the potential of ultrasonic treatment on the processing of polysaccharides as functional foods or food additives. The polysaccharide from Sinopodophyllum hexandrum fruit (SHP, 52.46 kDa, 1.91 nm) was isolated and purified. SHP was treated with various levels of ultrasound (250 W and 500 W), resulting...
Ultrasonic Treatment Enhances the Antioxidant and Immune-Stimulatory Properties of the Polysaccharide from Sinopodophyllum hexandrum Fruit We aimed to assess the potential of ultrasonic treatment on the processing of polysaccharides as functional foods or food additives. The polysaccharide from Sinopodophyllum hexandr...
PMC10001075
Wan Norizzati Wan Mohamad Zamri,Nazihah Mohd Yunus,Ahmad Aizat Abdul Aziz,Ninie Nadia Zulkipli,Sarina Sulong
Perspectives on the Application of Cytogenomic Approaches in Chronic Lymphocytic Leukaemia
03-03-2023
cytogenomics,chronic lymphocytic leukaemia,microarray
Chronic lymphocytic leukaemia (CLL) is a haematological malignancy characterised by the accumulation of monoclonal mature B lymphocytes (positive for CD5+ and CD23+) in peripheral blood, bone marrow, and lymph nodes. Although CLL is reported to be rare in Asian countries compared to Western countries, the disease cours...
Perspectives on the Application of Cytogenomic Approaches in Chronic Lymphocytic Leukaemia Chronic lymphocytic leukaemia (CLL) is a haematological malignancy characterised by the accumulation of monoclonal mature B lymphocytes (positive for CD5+ and CD23+) in peripheral blood, bone marrow, and lymph nodes. Although CL...
PMC10001080
Vladimir O. Sigin,Alexey I. Kalinkin,Alexandra F. Nikolaeva,Ekaterina O. Ignatova,Ekaterina B. Kuznetsova,Galina G. Chesnokova,Nikolai V. Litviakov,Matvey M. Tsyganov,Marina K. Ibragimova,Ilya I. Vinogradov,Maxim I. Vinogradov,Igor Y. Vinogradov,Dmitry V. Zaletaev,Marina V. Nemtsova,Sergey I. Kutsev,Alexander S. Tanas,...
DNA Methylation and Prospects for Predicting the Therapeutic Effect of Neoadjuvant Chemotherapy for Triple-Negative and Luminal B Breast Cancer
06-03-2023
breast cancer,DNA methylation,RRBS,MSRE-qPCR,predictive markers,neoadjuvant chemotherapy
Simple Summary Breast cancer (BC) is a group of diseases heterogeneous in morphology, progression, survival, and response to therapy. Although BC is among the most exhaustively studied cancers, there is still a lack of molecular markers to predict its response to neoadjuvant chemotherapy (NACT). Tumor development is de...
DNA Methylation and Prospects for Predicting the Therapeutic Effect of Neoadjuvant Chemotherapy for Triple-Negative and Luminal B Breast Cancer Breast cancer (BC) is a group of diseases heterogeneous in morphology, progression, survival, and response to therapy. Although BC is among the most exhaustively studied cance...
PMC10001084
Andressa Roehrig Volpe-Fix,Elias de França,Jean Carlos Silvestre,Ronaldo Vagner Thomatieli-Santos
The Use of Some Polyphenols in the Modulation of Muscle Damage and Inflammation Induced by Physical Exercise: A Review
21-02-2023
flavonoids,physical exercise,inflammation,muscle damage
Food bioactive compounds (FBC) comprise a vast class of substances, including polyphenols, with different chemical structures, and they exert physiological effects on individuals who consume them, such as antioxidant and anti-inflammatory action. The primary food sources of the compounds are fruits, vegetables, wines, ...
The Use of Some Polyphenols in the Modulation of Muscle Damage and Inflammation Induced by Physical Exercise: A Review Food bioactive compounds (FBC) comprise a vast class of substances, including polyphenols, with different chemical structures, and they exert physiological effects on individuals who consume them, suc...
PMC10001085
Albano Toska,Nikita Modi,Lin-Feng Chen
RUNX3 Meets the Ubiquitin-Proteasome System in Cancer
24-02-2023
E3 ligase,proteasomal degradation,RUNX3,tumor suppressor,ubiquitination
RUNX3 is a transcription factor with regulatory roles in cell proliferation and development. While largely characterized as a tumor suppressor, RUNX3 can also be oncogenic in certain cancers. Many factors account for the tumor suppressor function of RUNX3, which is reflected by its ability to suppress cancer cell proli...
RUNX3 Meets the Ubiquitin-Proteasome System in Cancer RUNX3 is a transcription factor with regulatory roles in cell proliferation and development. While largely characterized as a tumor suppressor, RUNX3 can also be oncogenic in certain cancers. Many factors account for the tumor suppressor function of RUNX3, which is...
PMC10001097
Jiahao Li,Lansi Chen,Jingjing Pang,Chunxiu Yang,Wen Xie,Guoyan Shen,Hongshan Chen,Xiaoyi Li,Shu-Yuan Xiao,Yueying Li
Autophagy-Related Gene WD Repeat Domain 45B Promotes Tumor Proliferation and Migration of Hepatocellular Carcinoma through the Akt/mTOR Signaling Pathway
27-02-2023
hepatocellular carcinoma,autophagy,prognosis,WDR45B,Akt/mTOR signaling pathway
Hepatocellular carcinoma (HCC) is a highly aggressive malignant tumor. It has been found that autophagy plays a role both as a tumor promoter and inhibitor in HCC carcinogenesis. However, the mechanism behind is still unveiled. This study aims to explore the functions and mechanism of the key autophagy-related proteins...
Autophagy-Related Gene WD Repeat Domain 45B Promotes Tumor Proliferation and Migration of Hepatocellular Carcinoma through the Akt/mTOR Signaling Pathway Hepatocellular carcinoma (HCC) is a highly aggressive malignant tumor. It has been found that autophagy plays a role both as a tumor promoter and inhibitor in HCC ca...
PMC10001101
Jessica Bamba-Funck,Emmanuelle E. Fabre,Marianne Kambouchner,Olivier Schischmanoff
Performance Characteristics of Oncomine Focus Assay for Theranostic Analysis of Solid Tumors, A (21-Months) Real-Life Study
01-03-2023
next generation sequencing (NGS),solid tumors,single nucleotide variation (SNV),copy number variation (CNV),fusion transcript,long-term follow-up
Next generation sequencing analysis is crucial for therapeutic decision in various solid tumor contexts. The sequencing method must remain accurate and robust throughout the instrument lifespan allowing the biological validation of patients’ results. This study aims to evaluate the long-term sequencing performances of ...
Performance Characteristics of Oncomine Focus Assay for Theranostic Analysis of Solid Tumors, A (21-Months) Real-Life Study Next generation sequencing analysis is crucial for therapeutic decision in various solid tumor contexts. The sequencing method must remain accurate and robust throughout the instrument lifespan a...
PMC10001105
Yohann Dabi,Amélia Favier,Léo Razakamanantsoa,Léa Delbos,Mathieu Poilblanc,Philippe Descamps,Francois Golfier,Cyril Touboul,Sofiane Bendifallah,Emile Daraï
Insight on Non-Coding RNAs from Biofluids in Ovarian Tumors
28-02-2023
ovarian tumor,non-coding RNA,borderline ovarian tumor,ovarian cancer
Simple Summary Ovarian cancer is the most lethal gynecologic cancer since it is often diagnosed at advanced stages. Current tools for diagnosis are currently insufficient and include physical examination, ultrasound and pelvic magnetic resonance imaging, as well as algorithms combining thoraco-abdomino-pelvic scans and...
Insight on Non-Coding RNAs from Biofluids in Ovarian Tumors Ovarian cancer is the most lethal gynecologic cancer since it is often diagnosed at advanced stages. Current tools for diagnosis are currently insufficient and include physical examination, ultrasound and pelvic magnetic resonance imaging, as well as algorith...
PMC10001109
Kholoud Almaabdi,Zareen Ahmad,Sindhu R. Johnson
Advanced Autoantibody Testing in Systemic Sclerosis
23-02-2023
systemic sclerosis,scleroderma,antibodies
Systemic sclerosis is a systemic autoimmune rheumatic disease characterized by immune abnormalities, leading to vasculopathy and fibrosis. Autoantibody testing has become an increasingly important part of diagnosis and prognostication. Clinicians have been limited to antinuclear antibody (ANA), antitopoisomerase I (als...
Advanced Autoantibody Testing in Systemic Sclerosis Systemic sclerosis is a systemic autoimmune rheumatic disease characterized by immune abnormalities, leading to vasculopathy and fibrosis. Autoantibody testing has become an increasingly important part of diagnosis and prognostication. Clinicians have been limited to...
PMC10001116
Giovanna Barbero,Roberta Zuntini,Pamela Magini,Laura Desiderio,Michela Bonaguro,Anna Myriam Perrone,Daniela Rubino,Mina Grippa,Antonio De Leo,Claudio Ceccarelli,Lea Godino,Sara Miccoli,Simona Ferrari,Donatella Santini,Pierandrea De Iaco,Claudio Zamagni,Giovanni Innella,Daniela Turchetti
Characterization of BRCA Deficiency in Ovarian Cancer
28-02-2023
BRCA1,BRCA2,ovarian cancer
Simple Summary Ovarian cancer (OC) is a highly lethal malignancy. Major improvements in treatment are expected from the identification of molecular features that may predict outcome or be used as therapeutic targets. Among genetic defects relevant for OC are those of BRCA1 and BRCA2 genes. Indeed, at least 20% of OC pa...
Characterization of BRCA Deficiency in Ovarian Cancer Ovarian cancer (OC) is a highly lethal malignancy. Major improvements in treatment are expected from the identification of molecular features that may predict outcome or be used as therapeutic targets. Among genetic defects relevant for OC are those of BRCA1 and BR...
PMC10001118
Yinuo Wang,Gergana Dobreva
Epigenetics in LMNA-Related Cardiomyopathy
01-03-2023
nuclear lamina,lamin A/C,LMNA,cardiomyopathy,epigenetics,chromatin architecture,stem cells
Mutations in the gene for lamin A/C (LMNA) cause a diverse range of diseases known as laminopathies. LMNA-related cardiomyopathy is a common inherited heart disease and is highly penetrant with a poor prognosis. In the past years, numerous investigations using mouse models, stem cell technologies, and patient samples h...
Epigenetics in LMNA-Related Cardiomyopathy Mutations in the gene for lamin A/C (LMNA) cause a diverse range of diseases known as laminopathies. LMNA-related cardiomyopathy is a common inherited heart disease and is highly penetrant with a poor prognosis. In the past years, numerous investigations using mouse models, s...
PMC10001120
Diane Frankel,Isabelle Nanni,L’Houcine Ouafik,Laurent Greillier,Hervé Dutau,Philippe Astoul,Laurent Daniel,Elise Kaspi,Patrice Roll
Cytological Samples: An Asset for the Diagnosis and Therapeutic Management of Patients with Lung Cancer
27-02-2023
cytological sample,cytopathology,lung cancer,adenocarcinoma,PD-L1,molecular testing,NGS,immunocytochemistry
Background: Lung cancer has become the leading cause of cancer death for men and women. Most patients are diagnosed at an advanced stage when surgery is no longer a therapeutic option. At this stage, cytological samples are often the less invasive source for diagnosis and the determination of predictive markers. We ass...
Cytological Samples: An Asset for the Diagnosis and Therapeutic Management of Patients with Lung Cancer Background: Lung cancer has become the leading cause of cancer death for men and women. Most patients are diagnosed at an advanced stage when surgery is no longer a therapeutic option. At this stage, cytological sam...
PMC10001121
Mariana de Moura e Dias,Vinícius da Silva Duarte,Lúcio Flávio Macedo Mota,Gabriela de Cássia Ávila Alpino,Sandra Aparecida dos Reis Louzano,Lisiane Lopes da Conceição,Hilário Cuquetto Mantovanie,Solange Silveira Pereira,Leandro Licursi Oliveira,Tiago Antônio de Oliveira Mendes,Davide Porcellato,Maria do Carmo Gouveia P...
Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy
03-03-2023
Lactobacillus gasseri LG-G12,intestinal health,ceftriaxone,gut microbiota,obesity
Gut microbiota imbalance is associated with the occurrence of metabolic diseases such as obesity. Thus, its modulation is a promising strategy to restore gut microbiota and improve intestinal health in the obese. This paper examines the role of probiotics, antimicrobials, and diet in modulating gut microbiota and impro...
Lactobacillus gasseri LG-G12 Restores Gut Microbiota and Intestinal Health in Obesity Mice on Ceftriaxone Therapy Gut microbiota imbalance is associated with the occurrence of metabolic diseases such as obesity. Thus, its modulation is a promising strategy to restore gut microbiota and improve intestinal health in the...
PMC10001133
Łukasz Witucki,Hieronim Jakubowski
Depletion of Paraoxonase 1 (Pon1) Dysregulates mTOR, Autophagy, and Accelerates Amyloid Beta Accumulation in Mice
26-02-2023
APP,amyloid beta,Pon1−/−5xFAD mouse model,N2a-APPswe cells,Pon1,homocysteine thiolactone,Phf8,H4K20me1,mTOR,autophagy
Paraoxonase 1 (PON1), a homocysteine (Hcy)-thiolactone detoxifying enzyme, has been associated with Alzheimer’s disease (AD), suggesting that PON1 plays an important protective role in the brain. To study the involvement of PON1 in the development of AD and to elucidate the mechanism involved, we generated a new mouse ...
Depletion of Paraoxonase 1 (Pon1) Dysregulates mTOR, Autophagy, and Accelerates Amyloid Beta Accumulation in Mice Paraoxonase 1 (PON1), a homocysteine (Hcy)-thiolactone detoxifying enzyme, has been associated with Alzheimer’s disease (AD), suggesting that PON1 plays an important protective role in the brain. To study ...
PMC10001145
Maik Luu,Burkhard Schütz,Matthias Lauth,Alexander Visekruna
The Impact of Gut Microbiota-Derived Metabolites on the Tumor Immune Microenvironment
03-03-2023
tumor microenvironment (TME),commensal bacteria,intratumoral microbiota,oncobiome,microbiota-derived metabolites,cancer immunotherapy
Simple Summary The tumor microenvironment (TME) comprises various non-malignant cells and soluble factors that surround cancer cells and which have mostly a pro-tumorigenic role. Growing evidence indicates that commensal bacteria are involved in the pathogenesis and progression but also in the suppression of various hu...
The Impact of Gut Microbiota-Derived Metabolites on the Tumor Immune Microenvironment The tumor microenvironment (TME) comprises various non-malignant cells and soluble factors that surround cancer cells and which have mostly a pro-tumorigenic role. Growing evidence indicates that commensal bacteria are involved in th...
PMC10001158
Ayse Sedef Köseer,Simona Di Gaetano,Claudia Arndt,Michael Bachmann,Anna Dubrovska
Immunotargeting of Cancer Stem Cells
05-03-2023
cancer stem cells,CSC,bsAB,CAR-T cells,cancer vaccines,immunotherapy
Simple Summary Tumor cells from the same specimen are functionally heterogeneous. Cancer stem cells (CSCs) are populations of tumor cells with self-renewal and differentiation properties. CSCs are found in nearly all solid and hematological tumors and are characterized by various surface or intracellular markers. These...
Immunotargeting of Cancer Stem Cells Tumor cells from the same specimen are functionally heterogeneous. Cancer stem cells (CSCs) are populations of tumor cells with self-renewal and differentiation properties. CSCs are found in nearly all solid and hematological tumors and are characterized by various surface or intra...
PMC10001161
Tristram A. J. Ryan,Luke A. J. O’Neill
An Emerging Role for Type I Interferons as Critical Regulators of Blood Coagulation
28-02-2023
type I interferons,blood coagulation,IFN-α,IFN-β,thrombin,PARs,haemostasis,thrombosis,tissue factor,SLE,APS,COVID-19,cGAS-STING,neutrophil extracellular traps,FXII
Type I interferons (IFNs) are central mediators of anti-viral and anti-bacterial host defence. Detection of microbes by innate immune cells via pattern recognition receptors (PRRs), including Toll-like receptors (TLRs) and cGAS-STING, induces the expression of type I IFN-stimulated genes. Primarily comprising the cytok...
An Emerging Role for Type I Interferons as Critical Regulators of Blood Coagulation Type I interferons (IFNs) are central mediators of anti-viral and anti-bacterial host defence. Detection of microbes by innate immune cells via pattern recognition receptors (PRRs), including Toll-like receptors (TLRs) and cGAS-STING, ...