triage_status large_stringclasses 28
values | pmid large_stringlengths 7 13 | abstract large_stringlengths 49 3.69k | citation large_stringlengths 16 84 | token_count int32 17 878 | label int8 0 1 |
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Cell composition or WT feature | PMID:2397448 | The patterns of end growth of individual cells of Schizosaccharomyces pombe, wild-type cells (strain 972 h-), cells exposed to 8 mM hydroxyurea, and cdc mutants (cdc11-123 and cdc2-33), were investigated by time-lapse photomicrography. It was reconfirmed that there are three patterns of end growth: cells growing at the... | Can J Microbiol 1990 Jun;36(6):390-4 | 223 | 0 |
Curatable | PMID:18662319 | The Schizosaccharomyces pombe ORF, SPAC29B12.10c, a predicted member of the oligopeptide transporter (OPT) family, was identified as a gene encoding the S. pombe glutathione transporter (Pgt1) by a genetic strategy that exploited the requirement of the cys1aDelta strain of S. pombe (which is defective in cysteine biosy... | FEMS Yeast Res 2008 Sep;8(6):916-29 | 337 | 1 |
Curatable, low priority | PMID:116193 | The sequence of tRNA tyrosine from the fission yeast Schizosaccharomyces pombe is pCUCCUGAUm1 GGUG psi AGDDGGDDAUCACACor (psi) CCGGUG psi Ai6 AACCGGUUGm7 GUm5C GCUAGT psi CGm1 AUUCUGGUCAGGAGACCAOH. This sequence differs in 30 nucleotides from the tRNA-Tyr seqence of the budding yeast Saccharomyces cerevisiae. It has a ... | Nucleic Acids Res 1979 Oct 25;7(4):1059-65 | 177 | 1 |
Method or reagent | PMID:10856773 | A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 micromol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans... | J Microbiol Methods 2000 Jun;41(1):19-21 | 110 | 0 |
Review or comment | PMID:23087902 | The notion that cellular membranes contain distinct microdomains, acting as scaffolds for signal transduction processes, has gained considerable momentum. In particular, a class of such domains that is rich in sphingolipids and cholesterol, termed as lipid rafts, is thought to compartmentalize the plasma membrane, and ... | Front Oncol 2012;2:140 | 361 | 0 |
Curatable | PMID:3870979 | We have investigated the genetic and physiological control of meiosis in fission yeast. Nutritionally depleted h+/h- diploid cells become irreversibly commited to meiosis immediately prior to the initiation of premeiotic S phase. Premeiotic DNA synthesis requires matP+, matM+, mei2+ and mei3+ but not the mitotic cell c... | Curr Genet 1985;10(4):297-311 | 303 | 1 |
Method or reagent | PMID:24947016 | In living organisms, most proteins work in complexes to form multicomponent protein machines. The function of such multicomponent machines is usually addressed by dividing them into a collection of two state systems at equilibrium. Many molecular machines, like Hsp90, work far from equilibrium by utilizing the energy o... | Nat Commun 2014 Jun 20;5:4192 | 209 | 0 |
Curatable | PMID:18257391 | The La protein interacts with a variety of small RNAs as well as certain growth-associated mRNAs such as Mdm2 mRNA. Human La (hLa) phosphoprotein is so highly conserved that it can replace the tRNA processing function of the fission yeast La protein in vivo. We used this system, which is based on tRNA-mediated suppress... | Gene Expr 2007;14(2):71-81 | 399 | 1 |
Curatable | PMID:9852944 | Both the rpb9 gene and its cDNA encoding the subunit 9 of RNA polymerase II were cloned from the fission yeast Schizosaccharomyces pombe. From the DNA sequences, Rpb9 was predicted to consist of 113 amino acid residues with a molecular mass of 13,175. S. pombe Rpb9 is 47, 40 and 36% identical in amino acid sequence to ... | Gene 1998 Oct 09;221(1):11-6 | 191 | 1 |
Curatable | PMID:32991693 | Amino acid deprivation or supplementation can affect cellular and organismal life span, but we know little about the role of concentration changes in free, intracellular amino acids during aging. Here, we determine free amino acid levels during chronological aging of nondividing fission yeast cells. We compare wild-typ... | J Gerontol A Biol Sci Med Sci 2021 01 18;76(2):205-210 | 231 | 1 |
Wrong organism | PMID:10209303 | The proper spatial and temporal coordination of mitosis and cytokinesis is essential for maintaining genomic integrity. We describe the identification and characterization of the Saccharomyces cerevisiae IBD1 gene, which encodes a novel protein that regulates the proper nuclear division and bud separation. IBD1 was ide... | Biochim Biophys Acta 1999 Apr 01;1449(3):239-53 | 459 | 0 |
Review or comment | PMID:26803803 | The spliceosome is formed on pre-mRNA substrates from five small nuclear ribonucleoprotein particles (U1, U2, U4/U6 and U5 snRNPs), and numerous non-snRNP factors. Saccharomyces cerevisiae U4/U6.U5 tri-snRNP comprises U5 snRNA, U4/U6 snRNA duplex and approximately 30 proteins and represents a substantial part of the sp... | Curr Opin Struct Biol 2016 Feb;36:48-57 | 298 | 0 |
Wrong organism | PMID:9451016 | The gene for the human dolichol cycle GlcNAc-1-P transferase (ALG7/GPT) was cloned by screening a human lung fibroblast cDNA library. The library was constructed in a Saccharomyces cerevisiae expression vector, and the positive clone was identified by complementation of the conditional lethal S.cerevisiae strain YPH-A7... | Glycobiology 1998 Jan;8(1):77-85 | 410 | 0 |
Curatable | PMID:10525840 | A Saccharomyces cerevisiae mutant affected in the last step of the biotin biosynthesis pathway was isolated by using a transposon mutagenesis method. The gene BIO2, encoding a biotin synthase, is shown to be interrupted in this mutant. Heterologous complementation experiment allowed the cloning and the characterization... | Curr Microbiol 1999 Dec;39(6):348-0350 | 97 | 1 |
Curatable | PMID:16541024 | Segregation of homologous maternal and paternal centromeres to opposite poles during meiosis I depends on post-replicative crossing over between homologous non-sister chromatids, which creates chiasmata and therefore bivalent chromosomes. Destruction of sister chromatid cohesion along chromosome arms due to proteolytic... | Nature 2006 May 04;441(7089):53-61 | 270 | 1 |
Curatable | PMID:10571085 | Proteins containing the baculovirus inhibitor of apoptosis repeats (BIR domains) have been identified in a wide range of species. BIR domain containing proteins are thought to inhibit caspases and thereby cause inhibition of apoptosis. A BIR domain containing protein has been recently identified by the Schizosaccharomy... | FEBS Lett 1999 Oct 22;460(1):187-90 | 247 | 1 |
Other | PMID:8146661 | The movement of chromosomes that precedes meiosis was observed in living cells of fission yeast by fluorescence microscopy. Further analysis by in situ hybridization revealed that the telomeres remain clustered at the leading end of premeiotic chromosome movement, unlike mitotic chromosome movement in which the centrom... | Science 1994 Apr 08;264(5156):270-3 | 160 | 0 |
Wrong organism | PMID:10329618 | In yeast, anaphase entry depends on Pds1 proteolysis, while chromosome re-duplication in the subsequent S-phase involves degradation of mitotic cyclins such as Clb2. Sequential proteolysis of Pds1 and mitotic cyclins is mediated by the anaphase-promoting complex (APC). Lagging chromosomes or spindle damage are detected... | EMBO J 1999 May 17;18(10):2707-21 | 233 | 0 |
Curatable | PMID:15791259 | Meiosis is a special form of nuclear division to generate eggs, sperm and spores in eukaryotes. Meiosis consists of the first (MI) and the second (MII) meiotic divisions, which occur consecutively. MI is reductional, in which homologous chromosomes derived from parents segregate. MI is supported by an elaborate mechani... | Nature 2005 Mar 24;434(7032):529-33 | 325 | 1 |
Curatable | PMID:24155978 | Glucose is one of the most important sources of cellular nutrition and glucose deprivation induces various cellular responses. In Schizosaccharomyces pombe, zinc finger protein Rst2 is activated upon glucose deprivation, and regulates gene expression via the STREP (stress response element of Schizosaccharomyces pombe) ... | PLoS One 2013;8(10):e78012 | 241 | 1 |
Curatable | PMID:22900017 | Gaf1 is the first GATA family zinc-finger transcription factor identified in Schizosaccharomyces pombe. Here, we report that Gaf1 functions as a negatively acting transcription factor of ste11(+), delaying the entrance of cells exposed to transient nitrogen starvation into the meiotic cycle. gaf1Δ strains exhibited acc... | PLoS One 2012;7(8):e42409 | 393 | 1 |
Curatable | PMID:28031482 | In this study we identified Pdc2, the fission yeast ortholog of human Pat1b protein, which forms a complex with Lsm1-7 and plays a role in coupling deadenylation and decapping. The involvement of Pdc2 in RNA degradation and P-body function was also determined. We found that Pdc2 interacts with Dcp2 and is required for ... | RNA 2017 04;23(4):493-503 | 396 | 1 |
Wrong organism | PMID:15130541 | The expression patterns of 18 FK506-binding proteins (FKBPs) encoded in the human genome have been established whereas the functional significance of the numerous ORFs coding for FKBP-like sequences remains unknown. Nominal masses of the human FKBPs vary from 12 to 135 kDa. Some large FKBPs consist up to four repeats o... | Comput Biol Chem 2004 Apr;28(2):129-40 | 433 | 0 |
Curatable | PMID:18003976 | Cellular protection against oxidative damage is relevant to ageing and numerous diseases. We analyzed the diversity of genome-wide gene expression programs and their regulation in response to various types and doses of oxidants in Schizosaccharomyces pombe. A small core gene set, regulated by the AP-1-like factor Pap1p... | Mol Biol Cell 2008 Jan;19(1):308-17 | 303 | 1 |
Cell composition or WT feature | PMID:17397989 | Local DNA melting is integral to fundamental processes such as replication or transcription. In vivo, these two processes do not occur on molecules free in solution but, instead, involve DNA molecules which are organized into DNA/proteins complexes. Atomic force microscopy imaging offers a possibility to look at indivi... | Biochimie 2007 Apr;89(4):534-41 | 306 | 0 |
Curatable | PMID:28893786 | We have previously reported that an erg11 mutation affecting ergosterol synthesis and a hem13 mutation in the heme synthesis pathway significantly sensitize the fission yeast Schizosaccharomyces pombe to hydroxyurea (HU) (1, 2). Here we show that treatment with inhibitors of Erg11 and heme biosynthesis phenocopie... | Antimicrob Agents Chemother 2017 11;61(11) | 263 | 1 |
Curatable | PMID:9167972 | In fission yeast, an RNA species named meiRNA is specifically required for the promotion of the first meiotic division. To dissect the function of this RNA and its partner RNA-binding protein Mei2, we screened for high-copy-number suppressors of the arrest prior to the first meiotic division caused by loss of meiRNA. A... | Genes Cells 1997 Feb;2(2):155-66 | 296 | 1 |
Curatable | PMID:10978278 | The biogenesis of microtubules in the cell comprises a series of complex steps, including protein-folding reactions catalyzed by chaperonins. In addition a group of evolutionarily conserved proteins, called cofactors (A to E), is required for the production of assembly-competent alpha-/beta-tubulin heterodimers. Using ... | Genetics 2000 Sep;156(1):93-103 | 304 | 1 |
Review or comment | PMID:26976145 | Cell division with accurate chromosome segregation is fundamental to cell survival of all organisms. The precise molecular mechanisms that ensure accurate chromosome segregation are still being discovered using a variety of experimental systems and approaches. Microtubule attachment to the kinetochore is a prerequisite... | Curr Genet 2016 Nov;62(4):691-695 | 182 | 0 |
Curatable | PMID:10467002 | It has been shown previously that amiloride, a widely used diuretic drug, inhibits growth in Schizosaccharomyces pombe. Here we show that the drug also alleviates repression by various nutrients of mating and sporulation in fission yeast. We selected spontaneous mutants that are amiloride-resistant and unable to mate a... | Curr Genet 1999 Jul;35(6):585-92 | 205 | 1 |
Wrong organism | PMID:23275568 | Human DNA polymerase mu (Polμ), a family X member involved in DNA repair, has both template-directed and terminal transferase (template-independent) activities. In addition to their ability to incorporate untemplated nucleotides, another similarity between Polµ and terminal deoxynucleotidyl transferase (TdT) is their p... | Nucleic Acids Res 2013 Feb 01;41(4):2428-36 | 281 | 0 |
Curatable | PMID:15340008 | In Schizosaccharomyces pombe, topoisomerase III is encoded by a single gene, top3(+), which is essential for cell viability and proper chromosome segregation. Deletion of rqh1(+), which encodes the sole RecQ family helicase in S. pombe, suppresses the lethality caused by loss of top3. Here, we provide evidence suggesti... | J Cell Sci 2004 Sep 15;117(Pt 20):4769-78 | 276 | 1 |
Curatable | PMID:10388808 | Transport of mRNA from the nucleus to the cytoplasm is one of the important steps in gene expression in eukaryotic cells. To elucidate a mechanism of mRNA export, we identified a novel ptr [poly(A)+ RNA transport] mutation, ptr6, which causes accumulation of mRNA in the nucleus and inhibition of growth at the nonpermis... | Genetics 1999 Jul;152(3):869-80 | 314 | 1 |
Wrong organism | PMID:15771425 | Elevated plasma aldosterone levels play a detrimental role in certain forms of congestive heart failure and myocardial fibrosis. We proposed aldosterone synthase (CYP11B2) as a novel target for the treatment of these diseases. In this study, the synthesis and biological evaluation of substituted E- and Z-imidazolylmeth... | J Med Chem 2005 Mar 24;48(6):1796-805 | 431 | 0 |
Wrong organism | PMID:1618302 | We have previously shown by cDNA cloning that a higher plant, Arabidopsis thaliana, possesses at least two CDC2 genes (CDC2a and CDC2b) similar to the cell-cycle-controlling cdc2 gene of Schizosaccharomyces pombe. To understand the exon-intron organization of these genes, genomic clones were isolated and their nucleoti... | FEBS Lett 1992 Jun 08;304(1):73-7 | 158 | 0 |
Curatable | PMID:19756689 | In the fission yeast, Schizosaccharomyces pombe, synaptonemal complexes (SCs) are not formed during meiotic prophase. However, structures resembling the axial elements of SCs, the so-called linear elements (LinEs) appear. By in situ immunostaining, we found Pmt3 (S. pombe's SUMO protein) transiently along LinEs, sugges... | Chromosoma 2010 Feb;119(1):59-72 | 329 | 1 |
Curatable | PMID:25533340 | Eukaryotic cells use two principal mechanisms for repairing DNA double-strand breaks (DSBs): homologous recombination (HR) and nonhomologous end-joining (NHEJ). DSB repair pathway choice is strongly regulated during the cell cycle. Cyclin-dependent kinase 1 (Cdk1) activates HR by phosphorylation of key recombination fa... | Cell Rep 2014 Dec 24;9(6):2011-7 | 243 | 1 |
Wrong organism | PMID:12548945 | A widespread interest has been noted in continuous power ethanol fermentation utilizing systems with immobilized yeast cell through self-flocculating. We studied continuous ethanol fermentation by a naturally flocculent strain of Schizosaccharomyces pombe and the performance of two-stage continuous ethanol fermentation... | Wei Sheng Wu Xue Bao 2000 Apr;40(2):198-203 | 159 | 0 |
Curatable | PMID:18256284 | In eukaryotic cells, proper formation of the spindle is necessary for successful cell division. We have studied chromosome recapture in the fission yeast Schizosaccharomyces pombe. We show by live cell analysis that lost kinetochores interact laterally with intranuclear microtubules (INMs) and that both microtubule dep... | Mol Biol Cell 2008 Apr;19(4):1646-62 | 242 | 1 |
Review or comment | PMID:19844171 | How do cells sense their own size and shape? And how does this information regulate progression of the cell cycle? Our group, in parallel to that of Paul Nurse, have recently demonstrated that fission yeast cells use a novel geometry-sensing mechanism to couple cell length perception with entry into mitosis. These rod-... | Cell Cycle 2009 Nov 15;8(22):3643-7 | 177 | 0 |
Wrong organism | PMID:25237103 | Ribonucleotide reductase (RNR) supplies the balanced pools of deoxynucleotide triphosphates (dNTPs) necessary for DNA replication and maintenance of genomic integrity. RNR is subject to allosteric regulatory mechanisms in all eukaryotes, as well as to control by small protein inhibitors Sml1p and Spd1p in budding and f... | Science 2014 Sep 19;345(6203):1512-5 | 214 | 0 |
Curatable | PMID:8960127 | Schizosaccharomyces pombe strains containing direct repeats of adeó heteroalleles separated by a functional uro4+ gene, and a DNA site for induction of a double-strand break (DSB), have been used to analyze pathways of spontaneous and DSB-induced intrachromosomal mitotic recombination. These substrates yield Ade+ Ura+ ... | Mutat Res 1996 Dec 02;364(3):14-60 | 323 | 1 |
Wrong organism | PMID:7593219 | Three major cyclin-dependent kinases, p34cdc2, p33cdk2, and p34cdk4 were examined in normal human T cells stimulated to enter the cell cycle in vitro. None of the three genes was expressed in resting T cells. Transcripts form the cdk4 and cdk2 genes were detectable as early as 3 and 8 hr after stimulation, respectively... | J Cell Physiol 1995 Nov;165(2):406-16 | 517 | 0 |
Curatable | PMID:10409726 | Myb-related cdc5p is required for G(2)/M progression in the yeast Schizosaccharomyces pombe. We report here that all detectable cdc5p is stably associated with a multiprotein 40S complex. Immunoaffinity purification has allowed the identification of 10 cwf (complexed with cdc5p) proteins. Two (cwf6p and cwf10p) are mem... | Mol Cell Biol 1999 Aug;19(8):5352-62 | 367 | 1 |
Wrong organism | PMID:8322511 | We isolated three highly homologous genes, PIR1, PIR2 and PIR3, collectively called the PIR genes. The remarkable feature of their putative amino acid sequence is that they contain a sequence consisting of 18-19 amino acid residues repeated tandemly seven to ten times. Genes homologous to PIR were found in Kluyveromyce... | Yeast 1993 May;9(5):481-94 | 259 | 0 |
Cell composition or WT feature | PMID:15237962 | Volatile compounds have been determined in control dry sherry wines and those supplemented with gluconic acid, which were inoculated with the Schizosaccharomyces pombe 1379 (ATCC 26760) yeast strain. These compounds were grouped, according to volatiles exhibiting the identical odor quality, into nine groups of the same... | J Agric Food Chem 2004 Jul 14;52(14):4529-34 | 207 | 0 |
Review or comment | PMID:9552418 | Most somatic cell division cycles contain a gap period (G2 phase) between the completion of DNA synthesis and the initiation of mitosis. This delay of mitotic entry is controlled, at least in part, by the repression of Cdc2 kinase activity by the phosphorylation of two conserved residues (Thr14 and Tyr15) within the AT... | Prog Cell Cycle Res 1997;3:233-40 | 164 | 0 |
Wrong organism | PMID:30617184 | Mitochondrial single-stranded DNA (ssDNA)-binding proteins (mtSSBs) are required for mitochondrial DNA replication and stability and are generally assumed to form homotetramers, and this species is proposed to be the one active for ssDNA binding. However, we recently reported that the mtSSB from Saccharomyces cerevisi... | J Biol Chem 2019 03 15;294(11):4137-4144 | 388 | 0 |
Curatable | PMID:11483497 | Modular domains mediating specific protein-protein interactions play central roles in the formation of complex regulatory networks to execute various cellular activities. Here we identify a novel domain PB1 in the budding yeast protein Bem1p, which functions in polarity establishment, and mammalian p67(phox), which act... | EMBO J 2001 Aug 01;20(15):3938-46 | 244 | 1 |
Method or reagent | PMID:21073853 | Homocitrate synthase (HCS) catalyzes the first step of l-lysine biosynthesis in fungi by condensing acetyl-coenzyme A and 2-oxoglutarate to form 3R-homocitrate and coenzyme A. Due to its conservation in pathogenic fungi, HCS has been proposed as a candidate for antifungal drug design. Here we report the development and... | Anal Biochem 2011 Mar 01;410(1):133-40 | 244 | 0 |
Curatable | PMID:15576943 | Eukaryotic cells regulate progression through the cell cycle in response to DNA damage. Cell cycle checkpoints are the signal transduction pathways that couple the detection of DNA damage to the proteins that control transitions in the cell cycle. The protein kinase Chk1, originally discovered in fission yeast, but con... | Methods Mol Biol 2005;296:345-54 | 170 | 1 |
Wrong organism | PMID:8006075 | In the fission yeast Schizosaccharomyces pombe, cdc2 function is required both in G1 to enter the cell cycle and in G2 to initiate mitosis. In higher eukaryotes, these functions appeared to be shared between several cdc2-like genes including CDK2. Temperature-sensitive mutations in S. pombe cdc2 that arrest the cell cy... | J Cell Sci 1994 Mar;107 ( Pt 3):615-23 | 380 | 0 |
Curatable | PMID:26240178 | During cytokinesis, fission yeast and other fungi and bacteria grow a septum that divides the cell in two. In fission yeast closure of the circular septum hole by the β-glucan synthases (Bgs) and other glucan synthases in the plasma membrane is tightly coupled to constriction of an actomyosin contractile ring attached ... | J Cell Sci 2015 Oct 01;128(19):3672-81 | 264 | 1 |
Loaded in error | PMID:32784463 | BACKGROUND: The extract of Adenostemma lavenia (L.) O. Kuntze leaves has anti-inflammatory activities and is used as a folk medicine to treat patients with hepatitis and pneumonia in China and Taiwan. The diterpenoid ent -11α-hydroxy-15-oxo-kaur-16-en-19-oic acid (11αOH-KA) is the major ingredient in the extract and... | Antioxidants (Basel) 2020 Aug 08;9(8) | 443 | 0 |
Curatable | PMID:21531710 | The histone H3 variant CENP-A is the most favored candidate for an epigenetic mark that specifies the centromere. In fission yeast, adjacent heterochromatin can direct CENP-A(Cnp1) chromatin establishment, but the underlying features governing where CENP-A(Cnp1) chromatin assembles are unknown. We show that, in additio... | J Biol Chem 2011 Jul 01;286(26):23600-7 | 232 | 1 |
Wrong organism | PMID:8991513 | The Saccharomyces cerevisiae mnn10 mutant is defective in the synthesis of N-linked oligosaccharides (Ballou et al., 1989). This mutation has no effect on O-linked sugars, but results in the accumulation of glycoproteins that contain severely truncated N-linked outer-chain oligosaccharides. We have cloned the MNN10 gen... | Glycobiology 1996 Jan;6(1):73-81 | 282 | 0 |
Wrong organism | PMID:15620353 | Posttranslational modifications of histone N-terminal tails impact chromatin structure and gene transcription. While the extent of histone acetylation is determined by both acetyltransferases and deacetylases, it has been unclear whether histone methylation is also regulated by enzymes with opposing activities. Here, w... | Cell 2004 Dec 29;119(7):941-53 | 227 | 0 |
Curatable | PMID:8553071 | Exposure of eukaryotic cells to agents that generate DNA damage results in transient arrest of progression through the cell cycle. In fission yeast, the DNA damage checkpoint associated with cell cycle arrest before mitosis requires the protein kinase p56chk1. DNA damage induced by ultraviolet light, gamma radiation, o... | Science 1996 Jan 19;271(5247):353-6 | 257 | 1 |
Method or reagent | PMID:7614549 | A disruption cassette has been constructed containing the LEU2 gene flanked by directly repeated site-specific recombination sites of the yeast plasmid, pSB3, which resembles the 2 microns DNA of Saccharomyces cerevisiae. A disruption constructed by inserting this DNA fragment acquires a Leu+ phenotype, which can be ea... | Curr Genet 1995 Mar;27(4):293-7 | 245 | 0 |
Curatable | PMID:30426027 | The DNA structure checkpoint protein Rad26 ATRIP is also required for an interphase microtubule damage response. This checkpoint delays spindle pole body separation and entry into mitosis following treatment of cells with microtubule poisons. This checkpoint requires cytoplasmic Rad26 ATRIP , which is compromised by t... | Mol Biol Res Commun 2018 Sep;7(3):97-106 | 312 | 1 |
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