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PMID:22426533
In addition to the neuronal and intestinal expression previously detected using an acs-1 promoter-driven GFP expression construct Kniazeva et al 2004 we observed prominent GFP fluorescence in the somatic gonad of adults but not in that of larvae Fig 2A B Supplemental Fig S2A–L S T
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PMID:22426533
Results from RNA in situ hybridization confirmed acs-1 expression in the somatic gonad while no expression was detected in the germline Supplemen- tal Fig S2M–R
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PMID:22426533
Microscopic evaluation of acs-1 gk3066 Ex acs-1 full T GFP adults revealed a prominent GFP fluorescence in the intestinal cells of all rescued animals n 500 whereas CAN neuron expression was only apparent in some Fig 2C D
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PMID:22426533
In wild-type early embryos ANI-1 :GFP localizes to the cortex before the first cleavage X Y and it is asymmetrically distributed when the embryo starts to form the cleavage furrow Z aa
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PMID:22426533
At the two-cell stage ANI-1 :GFP localizes to the dividing membrane and partitions to the cortex of the posterior cell ab ac
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PMID:22426533
In wild-type two-cell embryos NMY-2 :GFP is asymmetrically distributed between the daughter cells T
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PMID:22426533
The expression of acs-1 in the somatic gonad M-O but not in the germline P-R was detected
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PMID:22426533
M Dark grey staining in the intestine long arrows and in somatic gonad cells short arrows indicates hybridization of the anti-sense probe to the acs-1 transcript
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PMID:32668210
Note EFF-1 :GFP was not co-localized with plasma membrane marker myr :mKate2 before wounding but almost completely co-localized with the repaired membrane at the wound site
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PMID:32668210
EFF-1 was mainly localized to dynamic cytoplasmic vesicles but not with myr :mKate2 before wounding Figure S2A Video S1 consistent with the previous report that EFF-1 recycling from fused membrane is regulated by RAB-5-mediated endocytosis before during and after cell-cell fusion Smurova and Podbilewicz 2016 2017
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PMID:32668210
By generating a GFP reporter under the control of the syx-2 promoter we found that Psyx-2-GFP was expressed in the epidermis neurons and many other tissues Figure S4F
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PMID:32668210
We found that VPS-32 1 :GFP localized to cytosolic vesicles with a punctate pattern as having been shown previously Michelet et al 2009 while VPS- 4 :mKate2 was diffuse in the cytosol Figure S7E
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PMID:32668210
syx-2 is expressed in both epidermis and neurons
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PMID:34862187
Consistent with this the acox-1 1 gene is exclusively expressed in intestinal cells but not in D-type neurons
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PMID:34862187
At the young adult stage animals carrying Psrg-36 :srg-36 :sl2 :gfp did not show GFP expression in D-type moto neurons
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PMID:34816431
We first identified nmgp-1 expression in different amphid and phasmid neurons
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PMID:34816431
NMGP-1 also appeared to localize on the cell surface arrowheads in Figure 3a
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PMID:34816431
Furthermore a line scan measuring GFP intensity across the cell showed peaks consistent with plasma membrane localization Figure 3c indicating NGMP-1 presence on the plasma membrane
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PMID:34816431
nmgp-1 expresses mostly in sensory neurons and in the EGG-laying apparatus
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PMID:34816431
GFP expression driven by the putative nmgp-1 promoter was detected in several cells primarily neurons Figure 4
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PMID:34816431
nmgp-1 reporter expresses in sensory neurons and in vulva cells
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PMID:34816431
Cells labeled by Pnmgp-1 :GFP could be CAN and HSN neurons arrows
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PMID:34816431
Also in the medial region Pnmgp-1 :GFP labeled the dorsal cord arrowheads
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PMID:34816431
GFP expression in the egg asterisk indicates that nmgp-1 expresses early in the development
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PMID:34816431
Pnmgp-1 :GFP also labelled some cells in the tail
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PMID:34816431
Worm s pharynx ph nerve ring nr and head ganglia hg show GFP expression suggesting these cells normally express nmgp-1
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PMID:34816431
Overlapping right shows GFP expression under the nmgp-1 promoter within the egg-laying apparatus
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PMID:34816431
Arrows point to ventral cord cells expressing nmgp-1
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PMID:34816431
Arrows point to tail ganglia and indicate nmgp-1 expression
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PMID:34816431
We found that nmgp-1 was expressed in head and tail sensory neurons which might explain the altered response of nmgp-1 mutants in avoidance assays
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PMID:34816431
After detecting nmgp-1 reporter expression in the egg-laying apparatus we analyzed egg-laying in nmgp-1 mutants
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PMID:34816431
nmgp-1 appeared to be expressed in CEPsh cells
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PMID:35298637
HECD-1 shows ubiquitous expression in the cytoplasm throughout meiosis and early development
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PMID:35298637
A tagged HECD-1 allele showed ubiquitous embryonic expression
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PMID:35298637
HECD-1 is ubiquitously expressed in the embryo
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PMID:35298637
Using a fluorescent anti-FLAG antibody in fixed embryos expression of HECD-1 was ubiquitous in the cytoplasm of the developing embryo but was absent from the nucleus Fig 6a Supplementary Fig 5b
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PMID:35298637
We could detect no obvious colocalization of FLAG :HECD-1 with either meiotic or mitotic microtubules Fig 6b
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PMID:35298637
However embryonic FLAG :HECD-1 had ubiquitous cytosolic expression including during both meiosis and mitosis with no detectible enrichment on microtubules Fig 6
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PMID:35298637
Embryos had ubiquitous FLAG :HECD-1 localization in the cytoplasm but this was absent from the nucleus
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PMID:35297148
We obtained a cest-2 2p :GFP extrachromosomal array by microinjection and consistent with previous evidence Le et al 2020 observed high GFP expression levels primarily in the intestine Fig 3A
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PMID:35139370
A transcriptional reporter for nlp-18 STAR Methods revealed strong expression in the intestine and three pairs of neurons a gustatory sensory neuron ASI a mechanical sensory neuron FLP and an interneuron RIM Figures 2C and S2B
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PMID:35139370
A functional transcriptional reporter for CKR-1 revealed strong expression in many neurons and weak expression in the intestine Figure 4A
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PMID:35139370
CKR-1-expressing neurons include head motor neurons SMD and RME Figure 4B interneurons AIB and RIM peptidergic neurons RIS and body motor neurons A B and D
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PMID:35139370
Top ckr-1 expresses in the C elegans nervous system including the head and ventral cord neurons
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PMID:35139370
The arrows denote the expression of ckr-1 in ventral cord motor neurons
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PMID:35139370
B The Pckr-1 GFP expression in SMD neurons scale bar 20 mm
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PMID:35139370
The GFP driven by endogenous nlp-18 promoter was co-localized with RFP driven by Pgcy-13 in RIM neuron and by Pmec-3 in FLP neuron respectively
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PMID:35201977
Fluorescence from the mNeonGreen :QUI-1 fusion protein was confined to head and tail neurons and we observed expression in ASH ADL PHB and PVQ as previously reported Figure 1E and Hilliard et al 2004
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PMID:35201977
We observed expression in five additional neurons close to the nerve ring including possibly M3 AWB and ASJ and three neurons in the tail Figure 1E
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PMID:35201977
The mNeonGreen :QUI-1 fusion protein appears to be largely cytosolic and excluded from the nucleus consistent with previous reports Figure 1E and Neal et al 2016
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PMID:30953965
Using this transgenic strain we found that lips-6 is strongly expressed only in few cells in the vulva and tail while the rest of the worm tissues are devoid of signal or express lips-6 very faintly Fig 3C consistent with previous results using a non-integrated lips-6 GFP reporter 38
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PMID:25510497
We examined the subcellular localization of CBR-CSR-1 in embryos and found that it localizes to the nucleus mitotic chromosomes and centrosomes from the first mitotic cell division onward Figure 4A and B
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PMID:25510497
Interestingly we also observed localization of CBR-CSR-1 to the cleavage furrow during embryonic cell divisions a pattern that has not been described for C elegans Figure 4A and B
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PMID:25510497
In adult hermaphrodites CBR-CSR-1 was enriched in both germline and somatic nuclei Figure 4C and D
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PMID:25510497
We observed CBR-CSR-1 in association with oocyte nuclei and enriched on chromosomes in oocytes Figure 4C
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PMID:25510497
Again we did not reliably observe CSR-1 localization to perinuclear and cytoplasmic granules which would be indicative of P granules
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PMID:25510497
CBR-CSR-1 was also enriched in somatic gut nuclei further supporting a nuclear role for this Argonaute Figure 4D
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PMID:25510497
CBR-CSR-1 localizes to chromosomes and centrosomes in multiple stages of C briggsae development
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PMID:25510497
A CBR-CSR-1 localizes to mitotic chromatin white arrows prophase and metaphase stages are shown centrosomes yellow arrowheads and the cleavage furrow blue arrowheads in C briggsae embryos
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PMID:25510497
C CBR-CSR-1 localizes to chromatin in C briggsae oocytes
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PMID:25510497
D CBR-CSR-1 localizes to chromatin in C briggsae gut nuclei
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PMID:32305173
Our results indicated that ARGN-1 :mCh localized to mitochondria in the hypodermis and completely rescued the enlarged spherical mitochondria phenotype in argn- 1 yq187 mutants Fig 2B and F
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PMID:21390037
C elegans carrying a fat-7 :gfp reporter express FAT-7–GFP in intestinal cells where it is most strongly visible in the first anterior pair of cells Fig 2e top panel
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PMID:35324425
Using a fosmid-based reporter in which the ceh-33 locus is tagged with gfp we found that the CEH-33 protein shows no expression in the nervous system within or outside the pharynx at any developmental stage
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PMID:35324425
The ceh-34 reporter is turned on in the embryo at around the time of birth of pharyngeal neurons and is maintained in all pharyngeal neurons throughout all larval and adult stages Figure 2B
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PMID:35324425
We furthermore note that a pha-4 reporter allele that we generated through CRISPR Cas9 genome engineering is continuously expressed throughout the entire pharyngeal nervous system at all postembryonic stages
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PMID:35324425
We found that the spp-12 reporter is expressed in I4 and M4 neurons the flr-2 :SL2 :gfp :h2b reporter allele is expressed in five pharyngeal neuron classes I4 I5 M1 M4 and M5 and the htrl-1 :SL2 :gfp :h2b reporter allele is expressed in all pharyngeal neurons and in all other pharyngeal cells but nowhere outside the pharynx at the L1 stage Figure 6B
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PMID:35324425
A notable feature of the extrapharyngeal expression of the flr-2 reporter allele is expression in the AVL and DVB neurons Figure 6B the only extrapharyngeal neurons of the C elegans nervous system that innervate gut tissue not the foregut but the midgut White et al 1986
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PMID:35324425
We found that kin-36 indeed displays pan-pharyngeal neuron expression expression is also observed in all other pharyngeal cell types but no cell types outside the pharynx except some unidentified vulval cells Figure 6C
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PMID:35324425
We show that a C elegans ortholog of the Sine oculis Six1 Six2 homeobox gene ceh-34 is expressed in all pharyngeal neurons from their birth throughout their life time
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PMID:35324425
ceh-34 is expressed in all pharyngeal neurons
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PMID:35324425
ceh-33 fosmid reporter wgIs575 shows expression in a subset of head muscle cells
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PMID:35324425
str-97 otIs716 is expressed in M1 in adult animals but also in M4 in first larval stage animals
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PMID:35324425
rig-3 and rig-6 are expressed in almost all pharyngeal neurons plus many other cells within and outside the pharynx
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PMID:35324425
A eya-1 is expressed in all pharyngeal neurons throughout the life of the worm
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PMID:35324425
Images of L1 and adult worms showing co-localization of eya-1 expression nIs352 with the pan-neuronal gene rab-3 otIs355 in pharyngeal neurons
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PMID:34428327
Ce_MOB-4 is ubiquitously expressed in adult worms and the distribution is overlapped by that of Ce_YAP-1
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PMID:34843089
GFP :HDA-2 was strongly expressed in adult animals in the nerve ring pharynx distal tip cells sheath cells and uterus Fig 3c–h
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PMID:34843089
GFP :HDA-2 signals were detected during embryonic development at the bean stage and GFP :HDA-2 continuously expressed during hatching Fig 4
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PMID:34843089
We found that HDA-2 is strongly expressed in the sheath cells uterus distal tip cells nerve ring and pharynx
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PMID:34843089
In addition cells in the head and ventral regions strongly expressed HDA-2 at the 1 7-fold stage of the embryos
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PMID:22482727
We found that a Pmir-71 :gfp reporter was broadly expressed stronger signal was detected in the intestine body wall muscles and neurons during larval development and adulthood and weaker expression was observed in the hypoderm of adult animals Figure 3A Martinez et al 2008
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PMID:22629462
RSBP-1 is expressed in DA-receptive neurons that control locomotion
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PMID:22629462
Similar to a previous analysis of rsbp-1 expression 33 we found that the reporter transgene was expressed in head and tail neurons and motor neurons of the ventral cord that innervate body-wall muscle cells Figure 3A
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PMID:22629462
The rsbp-1p :GFP transgene was expressed in many ventral cord motor neurons Figure 3A
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PMID:22629462
We consistently found rsbp-1p :GFP expression in 45 ventral cord neurons between these ganglia indicating that RSBP-1 was expressed in both cholinergic and GABAergic motor neurons Figure 3A
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PMID:22629462
We found that rsbp-1p :GFP was coexpressed in the ventral cord neurons with this GABAergic-specific marker data not shown
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PMID:22629462
dop-1p :GFP is expressed in the cholinergic but not the GABAergic neurons of the ventral cord 25 and we found that the rsbp-1p :mCherry reporter transgene was coexpressed with dop- 1p :GFP in the cholinergic ventral cord neurons Figure 3B–E
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PMID:22629462
The results presented here indicate that RSBP-1 is expressed in GABAergic motor neurons with the DOP-3 receptor and is also expressed in cholinergic motor neurons with both DOP-1 and DOP-3 receptors
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PMID:22665789
daf-37 and daf-38 Are Expressed in Chemosensory Neurons
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PMID:22665789
Using transgenic worms expressing dsRED driven by the daf-37 promoter we determined that daf-37 is expressed in the ASI and ASK sensory neurons in the IL-2 interneurons and in the male- specific CEM cephalic sensilla male neurons which are involved in male mating behavior Fig 4A and Fig S5A 5 29
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PMID:22665789
Using anti- cMyc antibody we showed that DAF-37 was localized to the cilia Fig S5B
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PMID:22665789
A developmental immunoblot showed that DAF-37 is strongly expressed in L1 and L2 stages but is less abundant in the L3 L4 and adult stages
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PMID:22665789
We also detected DAF-37 in dauer larvae although the level of expression was lower than in any other stages Fig S5C
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PMID:22665789
Using a gfp-reporter construct we showed that DAF-38 was expressed in the same head neurons as DAF-37 Fig 4B but not in any other amphid cells
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PMID:22491324
MOD-1 and TDC-1 are coexpressed in RIM and RIC neurons
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PMID:22491324
We detected expression of mod-1 in RIM and RIC interneurons Fig 2e
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PMID:22491324
SER-2 and TPH-1 are coexpressed in nsm neurons
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PMID:32668210
Representative confocal image of EFF-1 :GFP single plane in the epidermal hyp7 before and after wounding
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PMID:35575797
FLN-2 is expressed in the hypodermis including the main body syncytium hyp7 and the smaller head and tail hypodermal cells Figs 1 M and M9 and S1 L–M9
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