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4,930 | https://bio-protocol.org/en/bpdetail?id=4930&type=0 | # Bio-Protocol Content
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A Protocol for Custom Biomineralization of Enzymes in Metal–Organic Frameworks (MOFs)
ZA Zoe Armstrong *
DJ Drew Jordahl *
AM Austin MacRae
QL Qiaobi Li
ML Mary Lenertz
PS Patrick Shen
AB Anastasiia Botserovska
LF Li Feng
AU ... |
4,931 | https://bio-protocol.org/en/bpdetail?id=4931&type=0 | # Bio-Protocol Content
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Streamlined Adeno-Associated Virus Production Using Suspension HEK293T Cells
AK Aditi A. Kulkarni
AS Austin G. Seal
CS Corinne Sonnet
KO Kazuhiro Oka
Published: Vol 14, Iss 3, Feb 5, 2024
DOI: 10.21769/BioProtoc.4931 Views: 1... |
4,932 | https://bio-protocol.org/en/bpdetail?id=4932&type=0 | # Bio-Protocol Content
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Use of the Fluorescent Dye Thioflavin T to Track Amyloid Structures in the Pathogenic Yeast Candida albicans
TM Thierry Mourer §
Cd Christophe d'Enfert
SB Sophie Bachellier-Bassi
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4,933 | https://bio-protocol.org/en/bpdetail?id=4933&type=0 | # Bio-Protocol Content
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SUrface SEnsing of Translation (SUnSET), a Method Based on Western Blot Assessing Protein Synthesis Rates in vitro
MP Marie Piecyk
JF Joëlle Fauvre
CD Cédric Duret
CC Cédric Chaveroux
CF Carole Ferraro-Peyret
Published: Vol 1... |
4,934 | https://bio-protocol.org/en/bpdetail?id=4934&type=0 | # Bio-Protocol Content
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Addressing the Role of Conventional CD8αβ+ T Cells and CD4+ T Cells in Intestinal Immunopathology Using a Bone Marrow–Engrafted Model
AA Amneh Aoudi
OL Ossama Labiad
RI Ramdane Igalouzene
OM Ousséma Mejri
MS Maxime Sanchez
SS... |
4,935 | https://bio-protocol.org/en/bpdetail?id=4935&type=0 | # Bio-Protocol Content
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CoCoNat: A Deep Learning–Based Tool for the Prediction of Coiled-coil Domains in Protein Sequences
MM Matteo Manfredi
CS Castrense Savojardo
PM Pier Luigi Martelli
RC Rita Casadio
Published: Vol 14, Iss 4, Feb 20, 2024
DOI: 1... |
4,936 | https://bio-protocol.org/en/bpdetail?id=4936&type=0 | # Bio-Protocol Content
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Generation of Human Induced Pluripotent Stem Cell (hiPSC)-Derived Astrocytes for Amyotrophic Lateral Sclerosis and Other Neurodegenerative Disease Studies
KD Katarina Stoklund Dittlau
AC Abinaya Chandrasekaran
KF Kristine Fre... |
4,937 | https://bio-protocol.org/en/bpdetail?id=4937&type=0 | # Bio-Protocol Content
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A Simple Immunofluorescence Method to Characterize Neurodegeneration and Tyrosine Hydroxylase Reduction in Whole Brain of a Drosophila Model of Parkinson’s Disease
RC Rahul Chaurasia *
MA Mohamad Ayajuddin *
GR Girish S. Ratn... |
4,938 | https://bio-protocol.org/en/bpdetail?id=4938&type=0 | # Bio-Protocol Content
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A Versatile Pipeline for High-fidelity Imaging and Analysis of Vascular Networks Across the Body
SV Stephen Vidman
ED Elliot Dion
AT Andrea Tedeschi
Published: Vol 14, Iss 4, Feb 20, 2024
DOI: 10.21769/BioProtoc.4938 Views: 1... |
4,939 | https://bio-protocol.org/en/bpdetail?id=4939&type=0 | # Bio-Protocol Content
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Unlocking Bio-Instructive Polymers: A Novel Multi-Well Screening Platform Based on Secretome Sampling
SF Shirin Fateh *
RA Reem A. Alromaihi *
AG Amir M. Ghaemmaghami
MA Morgan R. Alexander
(*contributed equally to this work... |
4,940 | https://bio-protocol.org/en/bpdetail?id=4940&type=0 | # Bio-Protocol Content
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Resolving the In Situ Three-Dimensional Structure of Fly Mechanosensory Organelles Using Serial Section Electron Tomography
LS Landi Sun *
JM Jana Meissner *
JH Jianfeng He
LC Lihong Cui
TF Tobias Fürstenhaupt
XL Xin Liang
(... |
4,941 | https://bio-protocol.org/en/bpdetail?id=4941&type=0 | # Bio-Protocol Content
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Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways
ZY Zilu Ye *
HW Hans H. Wandall
SD Sally Dabelsteen *
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Published: Vol 14, Iss 4, Feb 20, 2024
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4,942 | https://bio-protocol.org/en/bpdetail?id=4942&type=0 | # Bio-Protocol Content
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Correction Notice: Isolation and Culture of Murine Hepatic Stellate Cells
RM Rucha V. Modak
DZ Dietmar M. Zaiss
Published: Jan 20, 2024
DOI: 10.21769/BioProtoc.4942 Vie... |
4,943 | https://bio-protocol.org/en/bpdetail?id=4943&type=1 | # Bio-Protocol Content
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Analysis of DNA 5-methylcytosine Using Nanopore Sequencing
ZL Zhuowen Li
LZ Lihao Zheng
JZ Jixian Zhai
YL Yanping Long
Published: Feb 5, 2024
DOI: 10.21769/BioProtoc.4943 Views: 234
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4,944 | https://bio-protocol.org/en/bpdetail?id=4944&type=1 | # Bio-Protocol Content
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Utilisation of Methylome Data to Identify Stably Unmethylated Regions in Plant Genomes
JE Judith I. M. Eglitis-Sexton
LM Leroy M. Mangila
HA Haylie L. Andrews
LH Lee T. Hickey
PC Peter A. Crisp
Published: Feb 20, 2024
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4,945 | https://bio-protocol.org/en/bpdetail?id=4945&type=0 | # Bio-Protocol Content
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Mobilization of Plasmids from Bacteria into Diatoms by Conjugation Technique
FB Federico Berdun
MV Matías Valiñas
Gabriela Pagnussat
EZ Eduardo Zabaleta
Published: Vol 14, Iss 5, Mar 5, 2024
DOI: 10.21769/BioProtoc.4945 Views... |
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Analysis of Cleavage Activity of Dengue Virus Protease by Co-transfections
LG Lekha Gandhi
MV Musturi Venkataramana §
(§ Technical contact)
Published: Vol 14, Iss 5, Mar 5, 2024
DOI: 10.21769/BioProtoc.4946 Views: 408
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4,947 | https://bio-protocol.org/en/bpdetail?id=4947&type=0 | # Bio-Protocol Content
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Monitoring Intestinal Organoid–Derived Monolayer Barrier Functions with Electric Cell–Substrate Impedance Sensing (ECIS)
SO Sarah Ouahoud
FG Francesca P. Giugliano
... |
4,948 | https://bio-protocol.org/en/bpdetail?id=4948&type=0 | # Bio-Protocol Content
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Simple Rescue of Opaque Tissue Previously Cleared by iDISCO
HM Haylee Mesa *
JM Jonathan Meade *
PG Paula Gajewski-Kurdziel
RB Randy D. Blakely
QZ Qi Zhang
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Published: Vol 14, Iss 5, Mar 5,... |
4,949 | https://bio-protocol.org/en/bpdetail?id=4949&type=0 | # Bio-Protocol Content
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Real-Time Autophagic Flux Measurements in Live Cells Using a Novel Fluorescent Marker DAPRed
AS Arnold Sipos
KK Kwang-Jin Kim
JA Juan R. Alvarez
EC Edward D. Crandall
Published: Vol 14, Iss 5, Mar 5, 2024
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Immunofluorescent Staining Assay of 3D Cell Culture of Colonoids Isolated from Mice Colon
TM Trisha Mehrotra
XS Xiaodi Shi
DM Didier Merlin
Published: Vol 14, Iss 5, Mar 5, 2024
DOI: 10.21769/BioProtoc.4950 Views: 941
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Bacterial Pathogen-mediated Suppression of Host Trafficking to Lysosomes: Fluorescence Microscopy-based DQ-Red BSA Analysis
MM Mădălina Mocăniță
KM Kailey Martz
VD Vanessa M. D'Costa
Published: Vol 14, Iss 5, Mar 5, 2024
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An Optimized P. berghei Liver Stage–HepG2 Infection Model for Simultaneous Quantitative Bioimaging of Host and Parasite Nascent Proteomes
JM James L. McLellan
AG Andreu Garcia-Vilanova
KH Kirsten K. Hanson
Published: Vol 14, ... |
4,953 | https://bio-protocol.org/en/bpdetail?id=4953&type=1 | # Bio-Protocol Content
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Computational Analysis of Maize Enhancer Regulatory Elements Using ATAC-STARR-seq
AM Alexandre P. Marand
Published: Mar 5, 2024
DOI: 10.21769/BioProtoc.4953 Views: 156
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4,954 | https://bio-protocol.org/en/bpdetail?id=4954&type=1 | # Bio-Protocol Content
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Identification of Accessible Chromatin Regions with MNase-seq
CK Chuizheng Kong *
HP Hongcui Pei *
ZL Zefu Lu
LG Lifeng Gao
GZ Guangyao Zhao
XL Xu Liu
JJ Jizeng Jia
(*contributed equally to this work)
Published: Mar 20, 2024... |
4,955 | https://bio-protocol.org/en/bpdetail?id=4955&type=0 | # Bio-Protocol Content
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Classification of a Massive Number of Viral Genomes and Estimation of Time of Most Recent Common Ancestor (tMRCA) of SARS-CoV-2 Using Phylodynamic Analsysis
XH Xiaowen Hu *
SG Siqin Guan *
YH Yiliang He
GY Guohui Yi
LY Lei Ya... |
4,956 | https://bio-protocol.org/en/bpdetail?id=4956&type=0 | # Bio-Protocol Content
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Efficient Genetic Transformation and Suicide Plasmid-mediated Genome Editing System for Non-model Microorganism Erwinia persicina
TC Tingfeng Cheng §
TG Tongling Ge
XZ Xinyue Zhao
ZL Zhu Liu
LZ Lei Zhao
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4,957 | https://bio-protocol.org/en/bpdetail?id=4957&type=0 | # Bio-Protocol Content
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Mesenchymal Stromal Cell (MSC) Functional Analysis—Macrophage Activation and Polarization Assays
Hazel Y. Stevens
AJ Angela C. Jimenez
BW Bryan Wang
YL Ye Li
SS Shivaram Selvam
AB Annie C. Bowles-Welch
Published: Vol 14, Iss ... |
4,958 | https://bio-protocol.org/en/bpdetail?id=4958&type=0 | # Bio-Protocol Content
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Preparation and Purification of β-1,3-glucan-Linked Candida glabrata Cell Wall Proteases by Ion-Exchange Chromatography, Gel Filtration, and MDPF-Gelatin-Zymography Assay
PP Pirjo Pärnänen *
TS Timo Sorsa *
TT Taina Tervahart... |
4,959 | https://bio-protocol.org/en/bpdetail?id=4959&type=0 | # Bio-Protocol Content
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Dissecting the Mechanical Control of Mitotic Entry Using a Cell Confinement Setup
MD Margarida Dantas
DV Débora Vareiro
JF Jorge G. Ferreira
Published: Vol 14, Iss 6, Mar 20, 2024
DOI: 10.21769/BioProtoc.4959 Views: 1493
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496 | https://bio-protocol.org/en/bpdetail?id=496&type=0 | # Bio-Protocol Content
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Primary Culture of Cortical Neurons
Rieko Muramatsu
Toshihide Yamashita
Published: Vol 3, Iss 8, Apr 20, 2013
DOI: 10.21769/BioProtoc.496 Views: 25005
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4,960 | https://bio-protocol.org/en/bpdetail?id=4960&type=0 | # Bio-Protocol Content
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Genetic Knock-Ins of Endogenous Fluorescent Tags in RAW 264.7 Murine Macrophages Using CRISPR/Cas9 Genome Editing
BN Beverly Naigles §
JS Jan Soroczynski
NH Nan Hao
(§Technical contact: bnaigles@ucsd.edu)
Published: Vol 14, ... |
4,961 | https://bio-protocol.org/en/bpdetail?id=4961&type=0 | # Bio-Protocol Content
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Proximity Labelling to Quantify Kv7.4 and Dynein Protein Interaction in Freshly Isolated Rat Vascular Smooth Muscle Cells
JH Jennifer van der Horst
TJ Thomas A. Jepps
Published: Vol 14, Iss 6, Mar 20, 2024
DOI: 10.21769/BioPr... |
4,962 | https://bio-protocol.org/en/bpdetail?id=4962&type=0 | # Bio-Protocol Content
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From Llama to Nanobody: A Streamlined Workflow for the Generation of Functionalised VHHs
LE Lauren E.-A. Eyssen
SR Siva Ramadurai
SA Sahar Abdelkarim
IB Imogen Buckle
KC Katy Cornish
HL Hong Lin
AJ A.K. Jones
GS Gary J. Steph... |
4,963 | https://bio-protocol.org/en/bpdetail?id=4963&type=0 | # Bio-Protocol Content
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Fluorometric Measurement of Calmodulin-Dependent Peptide–Protein Interactions Using Dansylated Calmodulin
EN Eider Nuñez
AM Arantza Muguruza-Montero
SA Sara M. Alicante
AV Alvaro Villarroel
Published: Vol 14, Iss 7, Apr 5, 20... |
4,964 | https://bio-protocol.org/en/bpdetail?id=4964&type=0 | # Bio-Protocol Content
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Agrobacterium-Mediated Transient Gene Expression Optimized for the Bioenergy Crop Camelina sativa
PK Pawan Kumar
ZB Zeeshan Z. Banday
JR John L. Riley
JG Jean T. Greenberg
Published: Vol 14, Iss 7, Apr 5, 2024
DOI: 10.21769/B... |
4,965 | https://bio-protocol.org/en/bpdetail?id=4965&type=0 | # Bio-Protocol Content
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Method for Large-scale Production of hIPSC Spheroids
LL Lucas Lemarié
EC Edwin-Joffrey Courtial
JS Jérôme Sohier
Published: Vol 14, Iss 7, Apr 5, 2024
DOI: 10.21769/BioProtoc.4965 Views: 711
Reviewed by: Alessandro DidonnaLio... |
4,966 | https://bio-protocol.org/en/bpdetail?id=4966&type=0 | # Bio-Protocol Content
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Purification of Photorhabdus Virulence Cassette (PVC) Protein Complexes from Escherichia coli for Artificial Translocation of Heterologous Cargo Proteins
YW Yueying Wang *
XZ Xinting Zhang *
XF Xiao Feng
XW Xia Wang
QJ Qi Jin... |
4,967 | https://bio-protocol.org/en/bpdetail?id=4967&type=0 | # Bio-Protocol Content
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In-house Extraction and Purification of Pfu-Sso7d, a High-processivity DNA Polymerase
AM Aisha Mahboob *
NF Nishat Fatma *
AH Afzal Husain
(*contributed equally to this work)
Published: Vol 14, Iss 7, Apr 5, 2024
DOI: 10.217... |
4,968 | https://bio-protocol.org/en/bpdetail?id=4968&type=0 | # Bio-Protocol Content
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Transient Expression Assay and Microscopic Observation in Kumquat Fruit
JG Jinli Gong
XS Xuepeng Sun
Published: Vol 14, Iss 7, Apr 5, 2024
DOI: 10.21769/BioProtoc.4968 Views: 522
Reviewed by: Wenrong HeXiongjie ZhengMin Cao
D... |
4,969 | https://bio-protocol.org/en/bpdetail?id=4969&type=0 | # Bio-Protocol Content
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Nerve Preparation and Recordings for Pharmacological Tests of Sensory and Nociceptive Fiber Conduction Ex Vivo
VK Volodymyr Krotov
Olga Kopach
Published: Vol 14, Iss 7, Apr 5, 2024
DOI: 10.21769/BioProtoc.4969 Views: 422
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4,970 | https://bio-protocol.org/en/bpdetail?id=4970&type=0 | # Bio-Protocol Content
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Purification of Native Dentilisin Complex from Treponema denticola by Preparative Continuous Polyacrylamide Gel Electrophoresis and Functional Analysis by Gelatin Zymography
PK Pachiyappan Kamarajan
JT John C. Timm
MG M. Paul... |
4,971 | https://bio-protocol.org/en/bpdetail?id=4971&type=0 | # Bio-Protocol Content
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Automated Layer Analysis (ALAn): An Image Analysis Tool for the Unbiased Characterization of Mammalian Epithelial Architecture in Culture
CC Christian Cammarota
DB Dan T. Bergstralh
TF Tara M. Finegan
Published: Vol 14, Iss 8... |
4,972 | https://bio-protocol.org/en/bpdetail?id=4972&type=0 | # Bio-Protocol Content
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T-Cell-Based Platform for Functional Screening of T-Cell Receptors Identified in Single-Cell RNA Sequencing Data Sets of Tumor-Infiltrating T-Cells
AE Aaron Rodriguez Ehrenfried
SZ Stefan Zens
LS Laura K. Steffens
HK Hannes K... |
4,973 | https://bio-protocol.org/en/bpdetail?id=4973&type=0 | # Bio-Protocol Content
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Imaging Single-Cell Ca2+ Dynamics of Brainstem Neurons and Glia in Freely Behaving Mice
AB Amol M. Bhandare
ND Nicholas Dale
RH Robert T. R. Huckstepp
Published: Vol 14, Iss 8, Apr 20, 2024
DOI: 10.21769/BioProtoc.4973 Views:... |
4,974 | https://bio-protocol.org/en/bpdetail?id=4974&type=0 | # Bio-Protocol Content
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Protocol for the Implantation of Scaffolds in a Humanized Mouse Cutaneous Excisional Wound Healing Model
DG Dina Gadalla
MK Maeve M. Kennedy
DL David G. Lott
Published: Vol 14, Iss 18, Sep 20, 2024
DOI: 10.21769/BioProtoc.497... |
4,975 | https://bio-protocol.org/en/bpdetail?id=4975&type=0 | # Bio-Protocol Content
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Preparing and Evaluating the Stability of Therapeutically Relevant Oligonucleotide Duplexes
SI Shreyas G. Iyer §
AK Andrea L. Kasinski
(§ Technical contact)
Published: Vol 14, Iss 8, Apr 20, 2024
DOI: 10.21769/BioProtoc.4975... |
4,976 | https://bio-protocol.org/en/bpdetail?id=4976&type=0 | # Bio-Protocol Content
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CRISPR/dCas9-Tet1-Mediated DNA Methylation Editing
JQ Junming Qian
SL Shawn X. Liu
Published: Vol 14, Iss 8, Apr 20, 2024
DOI: 10.21769/BioProtoc.4976 Views: 1090
Reviewed by: Clara Morral MartinezPhilipp Wörsdörfer Anonymous... |
4,977 | https://bio-protocol.org/en/bpdetail?id=4977&type=0 | # Bio-Protocol Content
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Conditional Depletion of STN1 in Mouse Embryonic Fibroblasts
SK Sara Knowles
WC Weihang Chai
Published: Vol 14, Iss 8, Apr 20, 2024
DOI: 10.21769/BioProtoc.4977 Views: 2683
Reviewed by: Hélène LégerAYŞE NUR PEKTAŞ Anonymous r... |
4,978 | https://bio-protocol.org/en/bpdetail?id=4978&type=0 | # Bio-Protocol Content
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CRISPR/Cas9 Ribonucleoprotein-Mediated Mutagenesis in Sporisorium reilianum
JW Janina Werner
WZ Weiliang Zuo
Gunther Doehlemann
Published: Vol 14, Iss 8, Apr 20, 2024
DOI: 10.21769/BioProtoc.4978 Views: 681
Reviewed by: Shwet... |
4,979 | https://bio-protocol.org/en/bpdetail?id=4979&type=0 | # Bio-Protocol Content
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CD8α-CI-M6PR Particle Motility Assay to Study the Retrograde Motion of CI-M6PR Receptors in Cultured Living Cells
SR Shalini Rawat
Mahak Sharma
Published: Vol 14, Iss 9, May 5, 2024
DOI: 10.21769/BioProtoc.4979 Views: 372
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4,980 | https://bio-protocol.org/en/bpdetail?id=4980&type=0 | # Bio-Protocol Content
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A Novel Method for Floxed Gene Manipulation Using TAT-Cre Recombinase in Ex Vivo Precision-Cut Lung Slices (PCLS)
SC Sek-Shir Cheong
TL Tiago C. Luis
MH Matthew Hind
CD Charlotte H. Dean
Published: Vol 14, Iss 8, Apr 20, 2024... |
4,981 | https://bio-protocol.org/en/bpdetail?id=4981&type=0 | # Bio-Protocol Content
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Streamlining Protein Fractional Synthesis Rates Using SP3 Beads and Stable Isotope Mass Spectrometry: A Case Study on the Plant Ribosome
DG Dione Gentry-Torfer
EM Ester Murillo
CB Chloe L. Barrington
SN Shuai Nie
ML Michael G... |
4,982 | https://bio-protocol.org/en/bpdetail?id=4982&type=0 | # Bio-Protocol Content
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Apolipoprotein B Secretion Assay from Primary Hepatocytes
YW Yawei Wang
XL Xin Li
RH Runze Huang
XC Xiao-Wei Chen
XW Xiao Wang
Published: Vol 14, Iss 9, May 5, 2024
DOI: 10.21769/BioProtoc.4982 Views: 466
Reviewed by: Ralph T... |
4,983 | https://bio-protocol.org/en/bpdetail?id=4983&type=0 | # Bio-Protocol Content
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Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA Molecules
JC Jorge Cebrián
VM Victor Martínez
PH Pablo Hernández
DK Dora B. Krimer
MM María-Luisa Martínez-Robles
JS Jorge B. Schvartzman
MF M... |
4,984 | https://bio-protocol.org/en/bpdetail?id=4984&type=0 | # Bio-Protocol Content
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Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells
UK Urs Kindler
HZ Holm Zaehres
LM Lampros Mavrommatis
Published: Vol 14, Iss 9, May 5, 2024
DOI: 10.21769/BioProtoc.4984 Views: 1156
Reviewed by: Saman... |
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Updated Pseudo-seq Protocol for Transcriptome-Wide Detection of Pseudouridines
YP Yi Pan
HA Hironori Adachi
XH Xueyang He
JC Jonathan L. Chen
YY Yi-Tao Yu
PB Paul L. Boutz
Published: Vol 14, Iss 9, May 5, 2024
DOI: 10.21769/B... |
4,986 | https://bio-protocol.org/en/bpdetail?id=4986&type=0 | # Bio-Protocol Content
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Quantitative Measurement of Plasma Membrane Protein Internalisation and Recycling in Heterogenous Cellular Samples by Flow Cytometry
HL Hui Jing Lim
HM Hamish E. G. McWilliam
Published: Vol 14, Iss 9, May 5, 2024
DOI: 10.2176... |
4,987 | https://bio-protocol.org/en/bpdetail?id=4987&type=0 | # Bio-Protocol Content
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Simplified Protocol to Demonstrate Gene Expression in Nicotiana benthamiana Using an Agrobacterium-Mediated Transient Assay
SV Satyam Vergish
RW Ryan Wolf
WS Wen-Yuan Song
Published: Vol 14, Iss 10, May 20, 2024
DOI: 10.21769... |
4,988 | https://bio-protocol.org/en/bpdetail?id=4988&type=0 | # Bio-Protocol Content
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A Standardized Protocol for Extraction and Homogenization of Ocular Tissues
AH Anam Hammid
Published: Vol 14, Iss 10, May 20, 2024
DOI: 10.21769/BioProtoc.4988 Views: 1679
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Calcium Signal Analysis in the Zebrafish Heart via Phase Matching of the Cardiac Cycle
RZ Raymond Jiahong Zhang
JV Julien Vermot
RG Riccardo Gherardi
HF Hajime Fukui
Renee Wei-Yan Chow
Published: Vol 14, Iss 10, May 20, 2024
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Correction Notice: Isolation of Epithelial and Stromal Cells from Colon Tissues in Homeostasis and Under Inflammatory Conditions
CM Clara Morral *§
RG Reem Ghinnagow *
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Optogenetic Interrogation of Electrophysiological Dendritic Properties and Their Effect on Pacemaking Neurons from Acute Rodent Brain Slices
NG Naomi Gilin *
NW Nadine Wattad *
LT Lior Tiroshi
JG Joshua A. Goldberg
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A Detailed Protocol for the Induction of Anemia and RBC Transfusion–associated Necrotizing Enterocolitis in Neonatal Mice
BR Balamurugan Ramatchandirin
MB Marie Amalie Balamurugan
SD Suneetha Desiraju
YC Yerin Chung
KM Krishn... |
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Fluorescent Labeling and Imaging of IL-22 mRNA-Loaded Lipid Nanoparticles
RM Rabeya Jafrin Mow
AS Anand Srinivasan
EB Eunice Bolay
DM Didier Merlin
CY Chunhua Yang
Published: Vol 14, Iss 10, May 20, 2024
DOI: 10.21769/BioProt... |
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Reversible Photoregulation of Cell–Cell Adhesions With Opto-E-cadherin
CR Christopher A. Raab
SW Seraphine V. Wegner
Published: Vol 14, Iss 10, May 20, 2024
DOI: 10.21769/BioProtoc.4995 Views: 1300
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Rearing and Shipping of Uranotaenia lowii, a Frog-Biting Mosquito
RS Richa Singh
NS Neil D. Sanscrainte
AE Alden S. Estep
KG Katherine González
XB Ximena E. Bernal
Published: Vol 14, Iss 11, Jun 5, 2024
DOI: 10.21769/BioProto... |
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Visualising Neutrophil Actin Dynamics in Zebrafish in Response to Laser Wounding Using Two-Photon Microscopy
IW Ivanna Williantarra
AG Antonios Georgantzoglou
MS Milka Sarris
Published: Vol 14, Iss 11, Jun 5, 2024
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Linearly Amplified Single-Stranded RNA-Derived Transcriptome Sequencing (LAST-seq)
JL Jun Lyu
CC Chongyi Chen
Published: Vol 14, Iss 11, Jun 5, 2024
DOI: 10.21769/BioProtoc.4998 Views: 447
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Update Notice: TGIRT-seq Protocol for the Comprehensive Profiling of Coding and Non-coding RNA Biotypes in Cellular, Extracellular Vesicle, and Plasma RNAs
HX Hengyi Xu
RN R... |
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Immunophenotyping and Intracellular Staining of Fixed Whole Blood for Mass Cytometry (CyTOF)
SK Sangeeta Kowli
Holden Maecker
Published: Sep 5, 2020
DOI: 10.21769/BioProtoc.5004 Views: 5137
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Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein
AG Ankur Garg
LL Lihong Liu
DH David D. Ho
LJ Leemor Joshua-Tor
Published: Aug 5, 2020
DOI: 10.21769/BioProtoc.5005 Views: 4405
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Extraction of Orthologs from Genome-Sequencing Data for Phylogenetic Analysis
GP Guan Pang
FC Feng M. Cai
Published: Jun 5, 2024
DOI: 10.21769/BioProtoc.5008 Views: 84
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A Multi-Color Immunofluorescence Assay to Distinguish Intracellular From External Leishmania Parasites
AD Arani Datta
UB Umaru Barrie
DW Dawn M. Wetzel
Published: Vol 14, Iss 11, Jun 5, 2024
DOI: 10.21769/BioProtoc.5009 Views... |
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A New Approach for Assessment of Neutrophil Extracellular Traps Through Immunofluorescence Staining in Whole Blood Smears
SB Sakshi Bansal
Vinit Sharma
RG Rajesh Gupta
HS Harjeet Singh
AA Anjali Aggarwal
Published: Vol 14, Is... |
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Isolation and Characterization of Extracellular Vesicles Derived from Ex Vivo Culture of Visceral Adipose Tissue
AA Ankita Arora
Vinit Sharma
RG Rajesh Gupta
AA Anjali Aggarwal
Published: Vol 14, Iss 11, Jun 5, 2024
DOI: 10.2... |
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CRISPR-Cas9 Protocol for Efficient Gene Knockout and Transgene-free Plant Generation
EP Enzo A. Perk
AL Ana M. Laxalt
Ignacio Cerrudo
Published: Vol 14, Iss 11, Jun 5, 2024
DOI: 10.21769/BioProtoc.5012 Views: 1050
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Fast, Easy, and Comprehensive Techniques for Microscopic Observations of Fungal and Oomycete Organisms Inside the Roots of Herbaceous and Woody Plants
TT Tomáš Toma
JK Ján Kováč
Jaroslav Ďurkovič
Published: Vol 14, Iss 11, Ju... |
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Measuring Sleep and Activity Patterns in Adult Zebrafish
FD Fusun Doldur-Balli
AZ Amber J. Zimmerman
CS Christoph Seiler
OV Olivia Veatch
AP Allan I. Pack
Published: Vol 14, Iss 12, Jun 20, 2024
DOI: 10.21769/BioProtoc.5014 V... |
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Live Imaging of the Shoot Apical Meristem of Intact, Soil-Grown, Flowering Arabidopsis Plants
GB Gabriele Bradamante
Published: Vol 14, Iss 12, Jun 20, 2024
DOI: 10.21769/BioProtoc.5015 Views: 787
Reviewed by: Sarah C. Plecha... |
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Transfection of Babesia duncani: A Genetic Toolbox of This Pathogen to Advance Babesia Biology
SW Sen Wang
JW Jianyu Wang
DL Dongfang Li
FC Fangwei Chen
WL Wanxin Luo
JZ Junlong Zhao
LH Lan He
Published: Vol 14, Iss 12, Jun 2... |
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Direct RNA Sequencing of Foot-and-mouth Disease Virus Genome Using a Flongle on MinION
LX Lizhe Xu
AB Amy Berninger
SL Steven M. Lakin
VO Vivian O’Donnell
JP Jim L. Pierce
SP Steven J. Pauszek
RB Roger W. Barrette
BF Bonto Fa... |
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Flow Cytometry Analysis of Microglial Phenotypes in the Murine Brain During Aging and Disease
JC Jillian E. J. Cox
KP Kevin D. Pham
AK Alex W. Keck
ZW Zsabre Wright... |
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A Single-step Generation of AlissAID-based Conditional Knockdown Strains Using Nanobody that Targets GFP or mCherry in Budding Yeast
YO Yoshitaka Ogawa
TU Taisei P. Ueda
KO Keisuke Obara
KN Kohei Nishimura
TK Takumi Kamura
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Isolation of Human Bone Marrow Non-hematopoietic Cells for Single-cell RNA Sequencing
HL Hongzhe Li
SB Sandro Bräunig
SS Stefan Scheding
Published: Vol 14, Iss 12, Jun 20, 2024
DOI: 10.21769/BioProtoc.5020 Views: 646
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A Flow Cytometry–Based Method for Assessing CAR Cell Binding Kinetics Using Stable CAR Jurkat Cells
AS Alex Shepherd
BB Bigitha Bennychen
ZA Zafer Ahmed
RW Risini D. Weeratna
Scott McComb
Published: Vol 14, Iss 12, Jun 20, 20... |
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Bilateral Common Carotid Artery Stenosis in Mice: A Model of Chronic Cerebral Hypoperfusion-Induced Vascular Cognitive Impairment
MK Masashi Kakae
AK Ayaka Kawashita
HO Haruya Onogi
TN Takayuki Nakagawa
Hisashi Shirakawa
Publ... |
5,023 | https://bio-protocol.org/en/bpdetail?id=5023&type=0 | # Bio-Protocol Content
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Phylogenomics of Plant NLR Immune Receptors to Identify Functionally Conserved Sequence Motifs
TS Toshiyuki Sakai
AT AmirAli Toghani
HA Hiroaki Adachi
Published: Vol 14, Iss 13, Jul 5, 2024
DOI: 10.21769/BioProtoc.5023 Views:... |
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Quantification of Proliferating and Mitotically Active Retinal Cells in Mice by Flow Cytometry
HV Hope K. Vanzo-Sparks
SW Sarah E. Webster
MW Mark K. Webster
Cindy L. Linn
Published: Vol 14, Iss 13, Jul 5, 2024
DOI: 10.21769/... |
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Quantification of Autophagosomes in Human Fibroblasts Using Cyto-ID® Staining and Cytation Imaging
BH Barbara Hochecker
KM Katja C. Matt
AM Alica L. Meßmer
MS Melanie M. Scherer
JB Jörg Bergemann
Published: Vol 14, Iss 13, Ju... |
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Imaging Assays to Detect DNA Damage in Trypanosome Parasites Using γH2A
RJ Rajiv S. Jumani
BT Bryanna Thomas
SR Srinivasa P. S. Rao
Published: Vol 14, Iss 13, Jul 5, 2024
DOI: 10.21769/BioProtoc.5026 Views: 477
Reviewed by: M... |
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A Protocol for Preparing Mucoadhesive Emulsion Microgels and Assessing Their Mucoadhesion Properties In Vitro
MS Mariia S. Saveleva
ML Mikhail E. Lobanov
OM Oksana A. Mayorova
Published: Vol 14, Iss 13, Jul 5, 2024
DOI: 10.21... |
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Analysis and Quantification of the Mitochondrial–ER Lipidome
AD Alexis Diaz-Vegas
AD Anthony S. Don
JB James G. Burchfield
Published: Vol 14, Iss 13, Jul 5, 2024
DOI: 10.21769/BioProtoc.5028 Views: 858
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Versatile Cloning Strategy for Efficient Multigene Editing in Arabidopsis
ZL Ziqiang P. Li
JH Jennifer Huard
EB Emmanuelle M. Bayer
VW Valérie Wattelet-Boyer
Published: Vol 14, Iss 13, Jul 5, 2024
DOI: 10.21769/BioProtoc.5029... |
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Correction Notice: Live-Cell Imaging and Analysis of Germline Stem Cell Mitosis in Caenorhabditis elegans
RZ Réda M. Zellag
YZ Yifan Zhao
AG Abigail R. Gerhold
Publishe... |
5,031 | https://bio-protocol.org/en/bpdetail?id=5031&type=1 | # Bio-Protocol Content
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Workflow of Genome-Wide Mediation Analysis (GMA) by Incorporating Intermediate Omics Data as Mediators
ZY Zhikai Yang
QZ Qi Zhang
JY Jinliang Yang
Published: Jul 20, 2024
DOI: 10.21769/BioProtoc.5031 Views: 27
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5,032 | https://bio-protocol.org/en/bpdetail?id=5032&type=0 | # Bio-Protocol Content
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Purification and Cryo-Electron Microscopy Analysis of Bacterial Appendages
JS Juan C. Sanchez
JB Joseph K. Baumgardt
EW Elizabeth R. Wright
Published: Vol 14, Iss 14, Jul 20, 2024
DOI: 10.21769/BioProtoc.5032 Views: 1022
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5,033 | https://bio-protocol.org/en/bpdetail?id=5033&type=0 | # Bio-Protocol Content
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Analysis of Guard Cell Readouts Using Arabidopsis thaliana Isolated Epidermal Peels
RP Rosario Pantaleno
PS Paula Schiel
CG Carlos García-Mata
DS Denise Scuffi
Published: Vol 14, Iss 14, Jul 20, 2024
DOI: 10.21769/BioProtoc.5... |
5,034 | https://bio-protocol.org/en/bpdetail?id=5034&type=0 | # Bio-Protocol Content
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Characterizing ER Retention Defects of PDZ Binding Deficient Cx36 Mutants Using Confocal Microscopy
ST Stephan Tetenborg
EM Elizabeth Martinez-Soler
JO John O`Brien
Published: Vol 14, Iss 14, Jul 20, 2024
DOI: 10.21769/BioPro... |
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