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stats = Stats(self.config) return stats.usage(zone=self.zone, callback=callback, errback=errback, **kwargs)
def usage(self, callback=None, errback=None, **kwargs)
Return the current usage information for this zone :rtype: dict :return: usage information
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if not reload and self.data: raise MonitorException('monitor already loaded') def success(result, *args): self.data = result if callback: return callback(self) else: return self return self._rest.retrieve(...
def load(self, callback=None, errback=None, reload=False)
Load monitor data from the API.
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return self._rest.delete(self.data['id'], callback=callback, errback=errback)
def delete(self, callback=None, errback=None)
Delete the monitor
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if not self.data: raise MonitorException('monitor not loaded') def success(result, *args): self.data = result if callback: return callback(self) else: return self return self._rest.update(self.data['id'], ...
def update(self, callback=None, errback=None, **kwargs)
Update monitor configuration. Pass a list of keywords and their values to update.
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import ns1.rest.zones return ns1.rest.zones.Zones(self.config)
def zones(self)
Return a new raw REST interface to zone resources :rtype: :py:class:`ns1.rest.zones.Zones`
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import ns1.rest.records return ns1.rest.records.Records(self.config)
def records(self)
Return a new raw REST interface to record resources :rtype: :py:class:`ns1.rest.records.Records`
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import ns1.rest.ipam return ns1.rest.ipam.Addresses(self.config)
def addresses(self)
Return a new raw REST interface to address resources :rtype: :py:class:`ns1.rest.ipam.Adresses`
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import ns1.rest.ipam return ns1.rest.ipam.Networks(self.config)
def networks(self)
Return a new raw REST interface to network resources :rtype: :py:class:`ns1.rest.ipam.Networks`
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import ns1.rest.ipam return ns1.rest.ipam.Scopegroups(self.config)
def scope_groups(self)
Return a new raw REST interface to scope_group resources :rtype: :py:class:`ns1.rest.ipam.Scopegroups`
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import ns1.rest.stats return ns1.rest.stats.Stats(self.config)
def stats(self)
Return a new raw REST interface to stats resources :rtype: :py:class:`ns1.rest.stats.Stats`
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import ns1.rest.data return ns1.rest.data.Source(self.config)
def datasource(self)
Return a new raw REST interface to datasource resources :rtype: :py:class:`ns1.rest.data.Source`
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import ns1.rest.data return ns1.rest.data.Feed(self.config)
def datafeed(self)
Return a new raw REST interface to feed resources :rtype: :py:class:`ns1.rest.data.Feed`
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import ns1.rest.monitoring return ns1.rest.monitoring.Monitors(self.config)
def monitors(self)
Return a new raw REST interface to monitors resources :rtype: :py:class:`ns1.rest.monitoring.Monitors`
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import ns1.rest.monitoring return ns1.rest.monitoring.NotifyLists(self.config)
def notifylists(self)
Return a new raw REST interface to notify list resources :rtype: :py:class:`ns1.rest.monitoring.NotifyLists`
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import ns1.rest.account return ns1.rest.account.Plan(self.config)
def plan(self)
Return a new raw REST interface to account plan :rtype: :py:class:`ns1.rest.account.Plan`
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import ns1.zones zone = ns1.zones.Zone(self.config, zone) return zone.load(callback=callback, errback=errback)
def loadZone(self, zone, callback=None, errback=None)
Load an existing zone into a high level Zone object. :param str zone: zone name, like 'example.com' :rtype: :py:class:`ns1.zones.Zone`
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import ns1.zones return zone.search(q, has_geo, callback=callback, errback=errback)
def searchZone(self, zone, q=None, has_geo=False, callback=None, errback=None)
Search a zone for a given search query (e.g., for geological data, etc) :param zone: NOT a string like loadZone - an already loaded ns1.zones.Zone, like one returned from loadZone :return:
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import ns1.zones zone = ns1.zones.Zone(self.config, zone) return zone.create(zoneFile=zoneFile, callback=callback, errback=errback, **kwargs)
def createZone(self, zone, zoneFile=None, callback=None, errback=None, **kwargs)
Create a new zone, and return an associated high level Zone object. Several optional keyword arguments are available to configure the SOA record. If zoneFile is specified, upload the specific zone definition file to populate the zone with. :param str zone: zone name, like 'exam...
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import ns1.zones if zone is None: # extract from record string parts = domain.split('.') if len(parts) <= 2: zone = '.'.join(parts) else: zone = '.'.join(parts[1:]) z = ns1.zones.Zone(self.config, zone) ...
def loadRecord(self, domain, type, zone=None, callback=None, errback=None, **kwargs)
Load an existing record into a high level Record object. :param str domain: domain name of the record in the zone, for example \ 'myrecord'. You may leave off the zone, since it must be \ specified in the zone parameter :param str type: record type, such as 'A', 'MX', 'AAAA', et...
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import ns1.monitoring monitors_list = self.monitors().list(callback, errback) return [ns1.monitoring.Monitor(self.config, m) for m in monitors_list]
def loadMonitors(self, callback=None, errback=None, **kwargs)
Load all monitors
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import ns1.monitoring monitor = ns1.monitoring.Monitor(self.config) return monitor.create(callback=callback, errback=errback, **kwargs)
def createMonitor(self, callback=None, errback=None, **kwargs)
Create a monitor
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import ns1.ipam network = ns1.ipam.Network(self.config, id=id) return network.load(callback=callback, errback=errback)
def loadNetworkbyID(self, id, callback=None, errback=None)
Load an existing Network by ID into a high level Network object :param int id: id of an existing Network
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import ns1.ipam network = ns1.ipam.Network(self.config, name=name) return network.load(callback=callback, errback=errback)
def loadNetworkbyName(self, name, callback=None, errback=None)
Load an existing Network by name into a high level Network object :param str name: Name of an existing Network
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import ns1.ipam if scope_group_id is not None: scope_group = ns1.ipam.Scopegroup(self.config, id=scope_group_id).update() kwargs['scope_group'] = scope_group network = ns1.ipam.Network(self.config, name=name) return network.create(callback=callback, errba...
def createNetwork(self, name, scope_group_id=None, callback=None, errback=None, **kwargs)
Create a new Network For the list of keywords available, see :attr:`ns1.rest.ipam.Networks.INT_FIELDS` and :attr:`ns1.rest.ipam.Networks.PASSTHRU_FIELDS` :param str name: Name of the Network to be created :param int scope_group: (Optional) id of an existing scope group to associate with
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import ns1.ipam address = ns1.ipam.Address(self.config, id=id) return address.load(callback=callback, errback=errback)
def loadAddressbyID(self, id, callback=None, errback=None)
Load an existing address by ID into a high level Address object :param int id: id of an existing Address
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import ns1.ipam network = ns1.ipam.Network(self.config, id=network_id).load() address = ns1.ipam.Address(self.config, prefix=prefix, type=type, network=network) return address.load(callback=callback, errback=errback)
def loadAddressbyPrefix(self, prefix, type, network_id, callback=None, errback=None)
Load an existing address by prefix, type and network into a high level Address object :param str prefix: CIDR prefix of an existing Address :param str type: Type of address assignement (planned, assignment or host) :param int network_id: network_id associated with the address
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import ns1.ipam network = ns1.ipam.Network(self.config, id=network_id).load() address = ns1.ipam.Address(self.config, prefix=prefix, type=type, network=network) return address.create(callback=callback, errback=errback, **kwargs)
def createAddress(self, prefix, type, network_id, callback=None, errback=None, **kwargs)
Create a new Address For the list of keywords available, see :attr:`ns1.rest.ipam.Addresses.INT_FIELDS` and :attr:`ns1.rest.ipam.Addresses.PASSTHRU_FIELDS` :param str prefix: CIDR prefix of the address to be created :param str type: Type of address assignement (planned, assignment or host) ...
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import ns1.ipam scope_group = ns1.ipam.Scopegroup(self.config, id=id) return scope_group.load(callback=callback, errback=errback)
def loadScopeGroupbyID(self, id, callback=None, errback=None)
Load an existing Scope Group by ID into a high level Scope Group object :param int id: id of an existing ScopeGroup
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import ns1.ipam scope_group = ns1.ipam.Scopegroup(self.config, name=name, service_group_id=service_group_id) return scope_group.load(callback=callback, errback=errback)
def loadScopeGroupbyName(self, name, service_group_id, callback=None, errback=None)
Load an existing Scope Group by name and service group id into a high level Scope Group object :param str name: Name of an existing Scope Group :param int service_group_id: id of the service group the Scope group is associated with
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import ns1.ipam scope_group = ns1.ipam.Scopegroup(self.config, name=name, service_group_id=service_group_id) return scope_group.create(dhcp4=dhcp4, dhcp6=dhcp6, callback=callback, errback=errback)
def createScopeGroup(self, name, service_group_id, dhcp4, dhcp6, callback=None, errback=None)
Create a new Scope Group For the list of keywords available, see :attr:`ns1.rest.ipam.ScopeGroups.INT_FIELDS` and :attr:`ns1.rest.ipam.ScopeGroups.PASSTHRU_FIELDS` :param str name: Name of the Scope Group to be created :param int service_group_id: id of the service group the Scope group is asso...
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from ns1.ipam import DHCPOptions options = {} for option in DHCPOptions.OPTIONS[address_family]: options[option] = "" return options
def generateDHCPOptionsTemplate(self, address_family)
Generate boilerplate dictionary to hold dhcp options :param str address_family: dhcpv4 or dhcpv6 :return: dict containing valid option set for address family
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import ns1.ipam return ns1.ipam.DHCPOptions(address_family, options)
def loadDHCPOptions(self, address_family, options)
Create a high level DHCPOptions object :param str address_family: Address family of the options. Can be either dhcpv4 or dhcpv6 :param dict options: Dictionary containing the option set to apply for this address family. Note: only those specified will be applied. Allowed options can be found in :attr:`...
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if not comments: return it = iter(comments) first = next(it) extra_path_items = imap(_mark_as_root_path, first.root_path) return chain(extra_path_items, [first], it)
def fill_tree(comments)
Insert extra comments in the comments list, so that the root path of the first comment is always visible. Use this in comments' pagination to fill in the tree information. The inserted comments have an ``added_path`` attribute.
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if not comments: return it = iter(comments) # get the first item, this will fail if no items ! old = next(it) # first item starts a new thread old.open = True last = set() for c in it: # if this comment has a parent, store its last child for future reference ...
def annotate_tree_properties(comments)
iterate through nodes and adds some magic properties to each of them representing opening list of children and closing it
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'''Runs spades on all kmers. Each a separate run because SPAdes dies if any kmer does not work. Chooses the 'best' assembly to be the one with the biggest N50''' n50 = {} kmer_to_dir = {} for k in self.spades_kmers: tmpdir = tempfile.mkdtemp(prefix=self.outdir + '...
def run_spades(self, stop_at_first_success=False)
Runs spades on all kmers. Each a separate run because SPAdes dies if any kmer does not work. Chooses the 'best' assembly to be the one with the biggest N50
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'''Runs canu instead of spades''' cmd = self._make_canu_command(self.outdir,'canu') ok, errs = common.syscall(cmd, verbose=self.verbose, allow_fail=False) if not ok: raise Error('Error running Canu.') original_contigs = os.path.join(self.outdir, 'canu.contigs.fasta')...
def run_canu(self)
Runs canu instead of spades
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'''Returns Fasta or Fastq sequence from pysam aligned read''' if read.qual is None or ignore_quality: if qual is None or ignore_quality: seq = pyfastaq.sequences.Fasta(read.qname, common.decode(read.seq)) else: seq = pyfastaq.sequences.Fastq(read.qname, common.decode(read...
def aligned_read_to_read(read, revcomp=True, qual=None, ignore_quality=False)
Returns Fasta or Fastq sequence from pysam aligned read
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'''Gets the length of each reference sequence from the header of the bam. Returns dict name => length''' sam_reader = pysam.Samfile(self.bam, "rb") return dict(zip(sam_reader.references, sam_reader.lengths))
def _get_ref_lengths(self)
Gets the length of each reference sequence from the header of the bam. Returns dict name => length
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'''If contigs_to_use is a set, returns that set. If it's None, returns an empty set. Otherwise, assumes it's a file name, and gets names from the file''' if type(contigs_to_use) == set: return contigs_to_use elif contigs_to_use is None: return set() else: ...
def _get_contigs_to_use(self, contigs_to_use)
If contigs_to_use is a set, returns that set. If it's None, returns an empty set. Otherwise, assumes it's a file name, and gets names from the file
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'''Checks that the set of contigs to use are all in the reference fasta lengths dict made by self._get_ref_lengths()''' if self.contigs_to_use is None: return True for contig in self.contigs_to_use: if contig not in ref_dict: raise Error('Requeste...
def _check_contigs_to_use(self, ref_dict)
Checks that the set of contigs to use are all in the reference fasta lengths dict made by self._get_ref_lengths()
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'''Gets all reads from contig called "contig" and writes to fout''' sam_reader = pysam.Samfile(self.bam, "rb") for read in sam_reader.fetch(contig): print(mapping.aligned_read_to_read(read, ignore_quality=not self.fastq_out), file=fout)
def _all_reads_from_contig(self, contig, fout)
Gets all reads from contig called "contig" and writes to fout
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'''Writes all unmapped reads to fout''' sam_reader = pysam.Samfile(self.bam, "rb") for read in sam_reader.fetch(until_eof=True): if read.is_unmapped: print(mapping.aligned_read_to_read(read, ignore_quality=not self.fastq_out), file=fout)
def _get_all_unmapped_reads(self, fout)
Writes all unmapped reads to fout
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'''Get all reads from contig, but breaks them all at given position (0-based) in the reference. Writes to fout. Currently pproximate where it breaks (ignores indels in the alignment)''' sam_reader = pysam.Samfile(self.bam, "rb") for read in sam_reader.fetch(contig): seqs = [] ...
def _break_reads(self, contig, position, fout, min_read_length=250)
Get all reads from contig, but breaks them all at given position (0-based) in the reference. Writes to fout. Currently pproximate where it breaks (ignores indels in the alignment)
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'''Writes reads not mapping to the given region of contig, start and end as per python convention''' sam_reader = pysam.Samfile(self.bam, "rb") exclude_interval = pyfastaq.intervals.Interval(start, end - 1) for read in sam_reader.fetch(contig): read_interval = pyfastaq.interv...
def _exclude_region(self, contig, start, end, fout)
Writes reads not mapping to the given region of contig, start and end as per python convention
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'''Writes reads mapping to given region of contig, trimming part of read not in the region''' sam_reader = pysam.Samfile(self.bam, "rb") trimming_end = (start == 0) for read in sam_reader.fetch(contig, start, end): read_interval = pyfastaq.intervals.Interval(read.pos, read.re...
def _get_region(self, contig, start, end, fout, min_length=250)
Writes reads mapping to given region of contig, trimming part of read not in the region
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'''Returns a set of names from file called filename. If filename is None, returns an empty set''' if filename is None: return set() with open(filename) as f: return {line.rstrip() for line in f}
def _get_contigs_to_keep(self, filename)
Returns a set of names from file called filename. If filename is None, returns an empty set
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'''Writes a new file with small contigs removed. Returns lists of all names and names of removed contigs''' removed = set() all_names = set() if keep is None: keep = set() file_reader = pyfastaq.sequences.file_reader(infile) fout = pyfastaq.utils.o...
def _remove_small_contigs(self, infile, outfile, keep=None)
Writes a new file with small contigs removed. Returns lists of all names and names of removed contigs
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'''Returns two dictionaries: 1) name=>contig length. 2) Second is dictionary of nucmer hits (ignoring self matches). contig name => list of hits''' hits = {} lengths = {} file_reader = pymummer.coords_file.reader(infile) for al in file_reader: ...
def _load_nucmer_hits(self, infile)
Returns two dictionaries: 1) name=>contig length. 2) Second is dictionary of nucmer hits (ignoring self matches). contig name => list of hits
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'''Returns True iff (the query contig is contained in the reference contig and the query contig is not flagged to be kept)''' return ( hit.qry_name not in self.contigs_to_keep and hit.qry_name != hit.ref_name and (100 * hit.hit_length_qry / hit.qry_length >...
def _contains(self, hit)
Returns True iff (the query contig is contained in the reference contig and the query contig is not flagged to be kept)
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'''Given a list of hits, all with same query, returns a set of the contigs containing that query''' return {hit.ref_name for hit in hits if self._contains(hit)}
def _containing_contigs(self, hits)
Given a list of hits, all with same query, returns a set of the contigs containing that query
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'''Given dictionary of nucmer hits (made by self._load_nucmer_hits()), returns a dictionary. key=contig name. Value = set of contigs that contain the key.''' containing = {} for qry_name in hits_dict: d = self._containing_contigs(hits_dict[qry_name]) if len(d)...
def _get_containing_contigs(self, hits_dict)
Given dictionary of nucmer hits (made by self._load_nucmer_hits()), returns a dictionary. key=contig name. Value = set of contigs that contain the key.
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'''containing_contigs is a dict: key=contig name. Value = set of contigs that contain the key. Returns alls contigs called "name" that contain that contig''' contains_name = set() # failsafe to prevent infinite recursion if max_depth < 0: return con...
def _get_all_containing(self, containing_contigs, name, exclude=None, max_depth=10)
containing_contigs is a dict: key=contig name. Value = set of contigs that contain the key. Returns alls contigs called "name" that contain that contig
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'''This uses a contined in b, and b contained in c to force a contained in c. Just in case a contained in c wasn't already found by nucmer''' for name in containing_contigs: containing_contigs[name] = self._get_all_containing(containing_contigs, name) return containing_con...
def _expand_containing_using_transitivity(self, containing_contigs)
This uses a contined in b, and b contained in c to force a contained in c. Just in case a contained in c wasn't already found by nucmer
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'''Input is a list of sets. Merges any intersecting sets in the list''' found = True while found: found = False to_intersect = None for i in range(len(sets)): for j in range(len(sets)): if i == j: con...
def _collapse_list_of_sets(self, sets)
Input is a list of sets. Merges any intersecting sets in the list
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'''Input is a dict: key=contig name. Value = set of contigs that contain the key. Returns a list of sets of contigs that are equivalent''' equivalent_contigs = [] for qry_name, containing in hits_dict.items(): equivalent = set() for containing_nam...
def _get_identical_contigs(self, hits_dict)
Input is a dict: key=contig name. Value = set of contigs that contain the key. Returns a list of sets of contigs that are equivalent
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'''Returns the name of the longest contig, from the set of names contig_set. contig_lengths is expected to be a dictionary of contig name => length.''' longest_name = None max_length = -1 for name in contig_set: if contig_lengths[name] > max_length: ...
def _longest_contig(self, contig_set, contig_lengths)
Returns the name of the longest contig, from the set of names contig_set. contig_lengths is expected to be a dictionary of contig name => length.
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'''Input is dictionary of containing contigs made by self._expand_containing_using_transitivity(). Removes redundant identical contigs, leaving one representative (the longest) of each set of identical contigs. Returns new version of dictionary, and a dictionary of contig name =...
def _remove_identical_contigs(self, containing_contigs, contig_lengths)
Input is dictionary of containing contigs made by self._expand_containing_using_transitivity(). Removes redundant identical contigs, leaving one representative (the longest) of each set of identical contigs. Returns new version of dictionary, and a dictionary of contig name => contig it...
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'''Dies if any files in the list of filenames does not exist''' files_not_found = [x for x in filenames if not os.path.exists(x)] if len(files_not_found): for filename in files_not_found: print('File not found: "', filename, '"', sep='', file=sys.stderr) raise Error('File(s) not ...
def check_files_exist(filenames)
Dies if any files in the list of filenames does not exist
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'''Returns a dictionary of contig_name -> pyfastaq.Sequences.Fasta object''' contigs = {} pyfastaq.tasks.file_to_dict(self.contigs_fasta, contigs) return contigs
def get_contigs(self)
Returns a dictionary of contig_name -> pyfastaq.Sequences.Fasta object
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'''Returns a set of the contig names that are circular''' if self.assembler == 'spades': if self.contigs_fastg is not None: return self._circular_contigs_from_spades_before_3_6_1(self.contigs_fastg) elif None not in [self.contigs_paths, self.assembly_graph_fastg]:...
def circular_contigs(self)
Returns a set of the contig names that are circular
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'''Run nucmer of new assembly vs original assembly''' n = pymummer.nucmer.Runner( ref, qry, outfile, min_id=self.nucmer_min_id, min_length=self.nucmer_min_length, diagdiff=self.nucmer_diagdiff, maxmatch=True, ...
def _run_nucmer(self, ref, qry, outfile)
Run nucmer of new assembly vs original assembly
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'''Returns dict ref name => list of nucmer hits from infile''' hits = {} file_reader = pymummer.coords_file.reader(infile) for al in file_reader: if al.ref_name not in hits: hits[al.ref_name] = [] hits[al.ref_name].append(al) return hits
def _load_nucmer_hits(self, infile)
Returns dict ref name => list of nucmer hits from infile
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'''Input: list of nucmer hits. Output: dictionary, keys are query names, values are lists of hits''' d = {} for hit in hits: if hit.qry_name not in d: d[hit.qry_name] = [] d[hit.qry_name].append(hit) return d
def _hits_hashed_by_query(self, hits)
Input: list of nucmer hits. Output: dictionary, keys are query names, values are lists of hits
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'''Input: list of nucmer hits. Returns the longest hit, taking hit length on the reference''' if len(nucmer_hits) == 0: return None max_length = None longest_hit = None for hit in nucmer_hits: if max_length is None or hit.hit_length_ref > max_length: ...
def _get_longest_hit_by_ref_length(self, nucmer_hits)
Input: list of nucmer hits. Returns the longest hit, taking hit length on the reference
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2.01355
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'''Returns True iff the hit is "close enough" to the start of the reference sequence''' hit_coords = nucmer_hit.ref_coords() return hit_coords.start < self.ref_end_tolerance
def _is_at_ref_start(self, nucmer_hit)
Returns True iff the hit is "close enough" to the start of the reference sequence
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4.198872
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'''Returns True iff the hit is "close enough" to the end of the reference sequence''' hit_coords = nucmer_hit.ref_coords() return hit_coords.end >= nucmer_hit.ref_length - self.ref_end_tolerance
def _is_at_ref_end(self, nucmer_hit)
Returns True iff the hit is "close enough" to the end of the reference sequence
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3.419191
1.539773
'''Returns True iff the hit is "close enough" to the start of the query sequence''' hit_coords = nucmer_hit.qry_coords() return hit_coords.start < self.qry_end_tolerance
def _is_at_qry_start(self, nucmer_hit)
Returns True iff the hit is "close enough" to the start of the query sequence
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4.004255
1.693512
'''Returns True iff the hit is "close enough" to the end of the query sequence''' hit_coords = nucmer_hit.qry_coords() return hit_coords.end >= nucmer_hit.qry_length - self.qry_end_tolerance
def _is_at_qry_end(self, nucmer_hit)
Returns True iff the hit is "close enough" to the end of the query sequence
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3.262446
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'''Returns the hit nearest to the start of the ref sequence from the input list of hits''' nearest_to_start = hits[0] for hit in hits[1:]: if hit.ref_coords().start < nearest_to_start.ref_coords().start: nearest_to_start = hit return nearest_to_start
def _get_hit_nearest_ref_start(self, hits)
Returns the hit nearest to the start of the ref sequence from the input list of hits
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2.287696
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'''Returns the hit nearest to the end of the ref sequence from the input list of hits''' nearest_to_end = hits[0] for hit in hits[1:]: if hit.ref_coords().end > nearest_to_end.ref_coords().end: nearest_to_end = hit return nearest_to_end
def _get_hit_nearest_ref_end(self, hits)
Returns the hit nearest to the end of the ref sequence from the input list of hits
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2.332243
1.332426
'''Input: list of nucmer hits to the same reference. Returns the longest hit to the start of the reference, or None if there is no such hit''' if hits_to_exclude is None: hits_to_exclude = set() hits_at_start = [hit for hit in nucmer_hits if self._is_at_ref_start(hit) and hit not in ...
def _get_longest_hit_at_ref_start(self, nucmer_hits, hits_to_exclude=None)
Input: list of nucmer hits to the same reference. Returns the longest hit to the start of the reference, or None if there is no such hit
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1.4607
'''Input: list of nucmer hits to the same reference. Returns the longest hit to the end of the reference, or None if there is no such hit''' if hits_to_exclude is None: hits_to_exclude = set() hits_at_end = [hit for hit in nucmer_hits if self._is_at_ref_end(hit) and hit not in hits_t...
def _get_longest_hit_at_ref_end(self, nucmer_hits, hits_to_exclude=None)
Input: list of nucmer hits to the same reference. Returns the longest hit to the end of the reference, or None if there is no such hit
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2.015497
1.457237
'''Input: list of nucmer hits to the same query. Returns the longest hit to the start of the query, or None if there is no such hit''' hits_at_start = [hit for hit in nucmer_hits if self._is_at_qry_start(hit)] return self._get_longest_hit_by_ref_length(hits_at_start)
def _get_longest_hit_at_qry_start(self, nucmer_hits)
Input: list of nucmer hits to the same query. Returns the longest hit to the start of the query, or None if there is no such hit
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'''Input: list of nucmer hits to the same query. Returns the longest hit to the end of the query, or None if there is no such hit''' hits_at_end = [hit for hit in nucmer_hits if self._is_at_qry_end(hit)] return self._get_longest_hit_by_ref_length(hits_at_end)
def _get_longest_hit_at_qry_end(self, nucmer_hits)
Input: list of nucmer hits to the same query. Returns the longest hit to the end of the query, or None if there is no such hit
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'''Returns True iff list of nucmer_hits has a hit longer than min_length, not counting the hits in hits_to_exclude''' if hits_to_exclude is None: to_exclude = set() else: to_exclude = hits_to_exclude long_hits = [hit.hit_length_qry for hit in nucmer_hits if hit no...
def _has_qry_hit_longer_than(self, nucmer_hits, min_length, hits_to_exclude=None)
Returns True iff list of nucmer_hits has a hit longer than min_length, not counting the hits in hits_to_exclude
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2.025917
1.372956
'''Returns true iff the two hits can be used to circularise the reference sequence of the hits''' if not(self._is_at_ref_start(start_hit) or self._is_at_ref_end(end_hit)): return False if self._is_at_qry_end(start_hit) \ and self._is_at_qry_start(end_hit) \ and s...
def _can_circularise(self, start_hit, end_hit)
Returns true iff the two hits can be used to circularise the reference sequence of the hits
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1.971283
1.187668
'''Returns a dict ref name => tuple(hit at start, hit at end) for each ref sequence in the hash nucmer_hits (each value is a list of nucmer hits)''' writing_log_file = None not in [log_fh, log_outprefix] maybe_circular = {} all_nucmer_hits = [] for l in nucmer_hits.values(): ...
def _get_possible_circular_ref_contigs(self, nucmer_hits, log_fh=None, log_outprefix=None)
Returns a dict ref name => tuple(hit at start, hit at end) for each ref sequence in the hash nucmer_hits (each value is a list of nucmer hits)
2.215576
1.992395
1.112016
'''Returns a new dictionary, with keys from input dict removed if their value was not unique''' value_counts = collections.Counter(d.values()) new_d = {} writing_log_file = None not in [log_fh, log_outprefix] for key in d: if value_counts[d[key]] == 1: ...
def _remove_keys_from_dict_with_nonunique_values(self, d, log_fh=None, log_outprefix=None)
Returns a new dictionary, with keys from input dict removed if their value was not unique
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2.99803
1.256097
'''Given a nucmer ref_start_hit and ref_end_hit, returns a new contig. Assumes that these hits can be used to circularise the reference contig of the hits using the query contig''' assert ref_start_hit.ref_name == ref_end_hit.ref_name assert ref_start_hit.qry_name == ref_end_hit.qry_name ...
def _make_circularised_contig(self, ref_start_hit, ref_end_hit)
Given a nucmer ref_start_hit and ref_end_hit, returns a new contig. Assumes that these hits can be used to circularise the reference contig of the hits using the query contig
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1.827109
1.191502
'''Returns True iff the orientation of the hits means that the query contig of both hits can bridge the reference contigs of the hits''' assert start_hit.qry_name == end_hit.qry_name if start_hit.ref_name == end_hit.ref_name: return False if ( (self._is_at_ref_en...
def _orientation_ok_to_bridge_contigs(self, start_hit, end_hit)
Returns True iff the orientation of the hits means that the query contig of both hits can bridge the reference contigs of the hits
2.280079
1.757636
1.297242
'''Input is dict qry_name -> list of nucmer hits to that qry. Returns dict qry_name -> tuple(start hit, end hit)''' bridges = {} writing_log_file = None not in [log_fh, log_outprefix] for qry_name, hits_to_qry in nucmer_hits.items(): if len(hits_to_qry) < 2: ...
def _get_possible_query_bridging_contigs(self, nucmer_hits, log_fh=None, log_outprefix=None)
Input is dict qry_name -> list of nucmer hits to that qry. Returns dict qry_name -> tuple(start hit, end hit)
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2.218318
1.101962
'''Input is dict qry_name -> tuple(start hit, end hit) made by _get_possible_query_bridging_contigs. Removes key/values where the value(==ref contig) has a hit at the start, or at the end, to more than one key(==qry contig)''' ref_hits_start = {} ref_hits_end = {} for qry_name, (start_hi...
def _filter_bridging_contigs(self, bridges)
Input is dict qry_name -> tuple(start hit, end hit) made by _get_possible_query_bridging_contigs. Removes key/values where the value(==ref contig) has a hit at the start, or at the end, to more than one key(==qry contig)
2.45185
1.492749
1.642507
'''Input is dict of nucmer_hits. Makes any possible contig merges. Returns True iff any merges were made''' writing_log_file = None not in [log_fh, log_outprefix] if len(nucmer_hits) == 0: if writing_log_file: print(log_outprefix, 'No nucmer hits, so will ...
def _merge_all_bridged_contigs(self, nucmer_hits, ref_contigs, qry_contigs, log_fh=None, log_outprefix=None)
Input is dict of nucmer_hits. Makes any possible contig merges. Returns True iff any merges were made
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2.175909
1.1437
'''Writes crunch file and shell script to start up ACT, showing comparison of ref and qry''' if self.verbose: print('Making ACT files from', ref_fasta, qry_fasta, coords_file) ref_fasta = os.path.relpath(ref_fasta) qry_fasta = os.path.relpath(qry_fasta) coords_file = ...
def _write_act_files(self, ref_fasta, qry_fasta, coords_file, outprefix)
Writes crunch file and shell script to start up ACT, showing comparison of ref and qry
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2.484416
1.26236
'''Writes dictionary of contigs to file''' f = pyfastaq.utils.open_file_write(fname) for contig in sorted(contigs, key=lambda x:len(contigs[x]), reverse=True): print(contigs[contig], file=f) pyfastaq.utils.close(f)
def _contigs_dict_to_file(self, contigs, fname)
Writes dictionary of contigs to file
2.865892
2.907844
0.985573
'''Returns set of names of nodes in SPAdes fastg file that are circular. Names will match those in spades fasta file''' seq_reader = pyfastaq.sequences.file_reader(fastg) names = set([x.id.rstrip(';') for x in seq_reader if ':' in x.id]) found_fwd = set() found_rev = set() ...
def _get_spades_circular_nodes(self, fastg)
Returns set of names of nodes in SPAdes fastg file that are circular. Names will match those in spades fasta file
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2.559076
1.427772
'''Tries to make new circularised contig from contig called original_contig. hits = list of nucmer hits, all with ref=original contg. circular_spades=set of query contig names that spades says are circular''' writing_log_file = None not in [log_fh, log_outprefix] hits_to_circular_contigs = [x fo...
def _make_new_contig_from_nucmer_and_spades(self, original_contig, hits, circular_spades, log_fh=None, log_outprefix=None)
Tries to make new circularised contig from contig called original_contig. hits = list of nucmer hits, all with ref=original contg. circular_spades=set of query contig names that spades says are circular
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2.561841
1.181065
header_info = RE_HUNK_HEADER.match(header) hunk_info = header_info.groups() hunk = Hunk(*hunk_info) source_line_no = hunk.source_start target_line_no = hunk.target_start expected_source_end = source_line_no + hunk.source_length expected_target_end = targ...
def _parse_hunk(self, header, diff, encoding)
Parse hunk details.
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2.082341
1.008529
if (self.source_file.startswith('a/') and self.target_file.startswith('b/')): filepath = self.source_file[2:] elif (self.source_file.startswith('a/') and self.target_file == '/dev/null'): filepath = self.source_file[2:] elif (self.ta...
def path(self)
Return the file path abstracted from VCS.
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2.032784
1.077899
greedy = '?' if greedy else '' # For greedy search if isinstance(post, (list, tuple)): post = '(?=' + '|'.join(post) + ')' tag_list = re.findall(r'{pre}(.+{greedy}){post}'.format(pre=pre, post=post, greedy=greedy), string) if len(tag_list) > 1: raise Va...
def get_tag_value(string, pre, post, tagtype=float, greedy=True)
Extracts the value of a tag from a string. Parameters ----------------- pre : str regular expression to match before the the tag value post : str | list | tuple regular expression to match after the the tag value if list than the regular expressions will be combined into the ...
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3.137955
1.040986
# get dirname from user if not given if dirname is None: from FlowCytometryTools.gui import dialogs dirname = dialogs.select_directory_dialog('Select a directory') # find all files in dirname that match pattern if recursive: # search subdirs matches = [] for root, ...
def get_files(dirname=None, pattern='*.*', recursive=True)
Get all file names within a given directory those names match a given pattern. Parameters ---------- dirname : str | None Directory containing the datafiles. If None is given, open a dialog box. pattern : str Return only files whose names match the specified pattern. rec...
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2.860841
0.946846
with open(path, 'wb') as f: try: pickle.dump(obj, f, protocol=pickle.HIGHEST_PROTOCOL) except Exception as e: print('Pickling failed for object {0}, path {1}'.format(obj, path)) print('Error message: {0}'.format(e))
def save(obj, path)
Pickle (serialize) object to input file path Parameters ---------- obj : any object path : string File path
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f = open(path, 'rb') try: return pickle.load(f) finally: f.close()
def load(path)
Load pickled object from the specified file path. Parameters ---------- path : string File path Returns ------- unpickled : type of object stored in file
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if isinstance(obj, type(None)): return None elif isinstance(obj, six.string_types): return [obj] else: # Nesting here since symmetry is broken in isinstance checks. # Strings are iterables in python 3, so the relative order of if statements is important. if isins...
def to_iter(obj)
Convert an object to a list if it is not already an iterable. Nones are returned unaltered. This is an awful function that proliferates an explosion of types, please do not use anymore.
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1.105359
obj = to_iter(obj) if isinstance(obj, type(None)): return None else: return list(obj)
def to_list(obj)
Converts an object into a list if it not an iterable, forcing tuples into lists. Nones are returned unchanged.
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from copy import copy, deepcopy if deep: return deepcopy(self) else: return copy(self)
def copy(self, deep=True)
Make a copy of this object Parameters ---------- deep : boolean, default True Make a deep copy, i.e. also copy data Returns ------- copy : type of caller
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if th <= 0: raise ValueError('Threshold value must be positive. %s given.' % th) return where(x <= th, log10(th) * 1. * r / d, log10(x) * 1. * r / d)
def tlog(x, th=1, r=_display_max, d=_l_mmax)
Truncated log10 transform. Parameters ---------- x : num | num iterable values to be transformed. th : num values below th are transormed to 0. Must be positive. r : num (default = 10**4) maximal transformed value. d : num (default = log10(2**18)) log10 o...
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0.893347
if th <= 0: raise ValueError('Threshold value must be positive. %s given.' % th) x = 10 ** (y * 1. * d / r) try: x[x < th] = th except TypeError: if x < th: x = th return x
def tlog_inv(y, th=1, r=_display_max, d=_l_mmax)
Inverse truncated log10 transform. Values Parameters ---------- y : num | num iterable values to be transformed. th : num Inverse values below th are transormed to th. Must be > positive. r : num (default = 10**4) maximal transformed value. d : num (default =...
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4.798099
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aux = 1. * d / r * y s = sign(y) if s.shape: # to catch case where input is a single number s[s == 0] = 1 elif s == 0: s = 1 return s * 10 ** (s * aux) + b * aux - s
def hlog_inv(y, b=500, r=_display_max, d=_l_mmax)
Inverse of base 10 hyperlog transform.
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7.077213
1.043119
x = asarray(x) xmin = min(x) xmax = max(x) if xmin == xmax: return asarray([xmin] * nx) if xmax <= 0: # all values<=0 return -_x_for_spln(-x, nx, log_spacing)[::-1] if not log_spacing: return linspace(xmin, xmax, nx) # All code below is to handle-log-spacing w...
def _x_for_spln(x, nx, log_spacing)
Create vector of values to be used in constructing a spline. Parameters ---------- x : num | num iterable Resulted values will span the range [min(x), max(x)] nx : int Length of returned vector. log_spacing: bool False - Create linearly spaced values. True - Create l...
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3.29495
0.981974