chlange/streptococcus_thermophilus_fermentation_kaiser

Dataset Card for Raman Spectra from Fermentations of Streptococcus thermophilus Kaiser

Dataset Summary

This dataset contains offline Raman spectra collected during batch cultivations of Streptococcus thermophilus. The data is intended for researchers developing chemometric models and machine learning pipelines for bioprocess monitoring.

The dataset includes two distinct fermentation runs conducted in shake flasks over a 24-hour period, paired with reference analytical measurements (HPLC and spectrophotometry).

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Dataset Structure

Data Points

Each entry consists of a Raman spectrum (intensity vs. Raman shift) paired with the following quantitative annotations:

• Lactose [g/L]: Primary carbon source.
• Galactose [g/L]: Metabolic byproduct.
• Lactate [g/L]: Primary fermentation product.
• OD600: Optical density at 600 nm (biomass concentration).

Preprocessing

• Status: Raw.
• Note: The spectra are provided in their raw format without baseline correction, smoothing, or normalization (e.g., no SNV or Savitzky-Golay filtering has been applied).

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Technical Specifications

Cultivation Parameters

• Organism: Streptococcus thermophilus
• Cultivation Mode: Batch
• Vessel: Shake flask
• Duration: ~24 hours

Measurement Setup

• Spectrometer: Kaiser Raman RXN1
• Sampling: Offline samples taken throughout the fermentation.
• Reference Analytics: * Metabolites measured via High-Performance Liquid Chromatography (HPLC).
  • Biomass measured via UV-Vis Spectrophotometry ($OD_{600}$).

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Dataset Sources

• Repository: https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_kaiser
• Paper: [Link to paper if available]

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