Datasets:

chlange
/

streptococcus_thermophilus_fermentation_timegate

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Dataset Card for Time-Gated Raman Spectra from Fermentations of Streptococcus thermophilus

Dataset Summary

This dataset contains offline Raman spectra collected during batch cultivations of Streptococcus thermophilus. Unlike conventional continuous-wave Raman, these measurements were captured using Time-Gated Raman Spectroscopy, which allows for the temporal separation of the Raman signal from background fluorescence.

The dataset includes two distinct fermentation runs conducted in shake flasks over a 24-hour period, paired with reference analytical measurements (HPLC and spectrophotometry).

Dataset Structure

Data Points

Each entry consists of a Raman spectrum (intensity vs. Raman shift) paired with the following quantitative annotations:

Lactose [g/L]: Primary carbon source.
Galactose [g/L]: Metabolic byproduct.
Lactate [g/L]: Primary fermentation product.
OD600: Optical density at 600 nm (biomass concentration).

Preprocessing

Status: Raw.
Note: The spectra are provided in their raw format. No computational fluorescence rejection, baseline correction, or smoothing has been applied. The time-gating hardware provides the initial fluorescence suppression.

Technical Specifications

Cultivation Parameters

Organism: Streptococcus thermophilus
Cultivation Mode: Batch
Vessel: Shake flask
Duration: ~24 hours

Measurement Setup

Spectrometer: Timegate PicoRaman (First Generation)
Technology: Time-Gated Raman Spectroscopy (pulsed laser)
Sampling: Offline samples taken throughout the fermentation.
Reference Analytics: * Metabolites measured via High-Performance Liquid Chromatography (HPLC).
- Biomass measured via UV-Vis Spectrophotometry ($OD_{600}$).

Dataset Sources

Repository: https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_timegate
Paper: [Link to paper if available]

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