Update README.md

README.md

@@ -1,3 +1,65 @@
----
-license: cc-by-4.0
----
+---
+license: cc-by-4.0
+tags:
+- raman-spectroscopy
+- time-gated-raman
+- biotechnology
+- streptococcus-thermophilus
+- fermentation
+- batch-cultivation
+- process-analytical-technology
+pretty_name: Time-Gated Raman Spectra of Streptococcus thermophilus Fermentations
+language:
+- en
+size_categories:
+- n<1K
+---
+
+# Dataset Card for Time-Gated Raman Spectra from Fermentations of Streptococcus thermophilus
+
+## Dataset Summary
+This dataset contains offline Raman spectra collected during batch cultivations of ***Streptococcus thermophilus***. Unlike conventional continuous-wave Raman, these measurements were captured using **Time-Gated Raman Spectroscopy**, which allows for the temporal separation of the Raman signal from background fluorescence.
+
+The dataset includes two distinct fermentation runs conducted in shake flasks over a 24-hour period, paired with reference analytical measurements (HPLC and spectrophotometry).
+
+---
+
+## Dataset Structure
+
+### Data Points
+Each entry consists of a Raman spectrum (intensity vs. Raman shift) paired with the following quantitative annotations:
+
+* **Lactose [g/L]:** Primary carbon source.
+* **Galactose [g/L]:** Metabolic byproduct.
+* **Lactate [g/L]:** Primary fermentation product.
+* **OD600:** Optical density at 600 nm (biomass concentration).
+
+### Preprocessing
+* **Status:** Raw.
+* **Note:** The spectra are provided in their raw format. No computational fluorescence rejection, baseline correction, or smoothing has been applied. The time-gating hardware provides the initial fluorescence suppression.
+
+---
+
+## Technical Specifications
+
+### Cultivation Parameters
+* **Organism:** *Streptococcus thermophilus*
+* **Cultivation Mode:** Batch
+* **Vessel:** Shake flask
+* **Duration:** ~24 hours
+
+### Measurement Setup
+* **Spectrometer:** Timegate PicoRaman (First Generation)
+* **Technology:** Time-Gated Raman Spectroscopy (pulsed laser)
+* **Sampling:** Offline samples taken throughout the fermentation.
+* **Reference Analytics:** * Metabolites measured via High-Performance Liquid Chromatography (HPLC).
+    * Biomass measured via UV-Vis Spectrophotometry ($OD_{600}$).
+
+---
+
+## Dataset Sources
+
+- **Repository:** [https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_timegate](https://huggingface.co/datasets/chlange/streptococcus_thermophilus_fermentation_timegate)
+- **Paper:** [Link to paper if available]
+
+---