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When we used these same parameters earlier, we saw the network with batch normalization reach 92% validation accuracy. This time we used different starting weights, initialized using the same standard deviation as before, and the network doesn't learn at all. (Remember, an accuracy around 10% is what the network gets i...
train_and_test(True, 2, tf.nn.relu)
batch-norm/Batch_Normalization_Lesson.ipynb
JasonNK/udacity-dlnd
mit
When we trained with these parameters and batch normalization earlier, we reached 90% validation accuracy. However, this time the network almost starts to make some progress in the beginning, but it quickly breaks down and stops learning. Note: Both of the above examples use extremely bad starting weights, along with ...
def fully_connected(self, layer_in, initial_weights, activation_fn=None): """ Creates a standard, fully connected layer. Its number of inputs and outputs will be defined by the shape of `initial_weights`, and its starting weight values will be taken directly from that same parameter. If `self.use_batch_...
batch-norm/Batch_Normalization_Lesson.ipynb
JasonNK/udacity-dlnd
mit
This version of fully_connected is much longer than the original, but once again has extensive comments to help you understand it. Here are some important points: It explicitly creates variables to store gamma, beta, and the population mean and variance. These were all handled for us in the previous version of the fun...
def batch_norm_test(test_training_accuracy): """ :param test_training_accuracy: bool If True, perform inference with batch normalization using batch mean and variance; if False, perform inference with batch normalization using estimated population mean and variance. """ weights = [np.ra...
batch-norm/Batch_Normalization_Lesson.ipynb
JasonNK/udacity-dlnd
mit
In the following cell, we pass True for test_training_accuracy, which performs the same batch normalization that we normally perform during training.
batch_norm_test(True)
batch-norm/Batch_Normalization_Lesson.ipynb
JasonNK/udacity-dlnd
mit
As you can see, the network guessed the same value every time! But why? Because during training, a network with batch normalization adjusts the values at each layer based on the mean and variance of that batch. The "batches" we are using for these predictions have a single input each time, so their values are the means...
batch_norm_test(False)
batch-norm/Batch_Normalization_Lesson.ipynb
JasonNK/udacity-dlnd
mit
Now let's add a mesh dataset at a few different times so that we can see how the potentials affect the surfaces of the stars.
b.add_dataset('mesh', times=np.linspace(0,1,11), dataset='mesh01')
2.3/tutorials/requiv.ipynb
phoebe-project/phoebe2-docs
gpl-3.0
Relevant Parameters The 'requiv' parameter defines the stellar surface to have a constant volume of 4./3 pi requiv^3.
print(b['requiv@component'])
2.3/tutorials/requiv.ipynb
phoebe-project/phoebe2-docs
gpl-3.0
Critical Potentials and System Checks Additionally, for each detached component, there is an requiv_max Parameter which shows the critical value at which the Roche surface will overflow. Setting requiv to a larger value will fail system checks and raise a warning.
print(b['requiv_max@primary@component']) print(b['requiv_max@primary@constraint']) b.set_value('requiv@primary@component', 3)
2.3/tutorials/requiv.ipynb
phoebe-project/phoebe2-docs
gpl-3.0
At this time, if you were to call run_compute, an error would be thrown. An error isn't immediately thrown when setting requiv, however, since the overflow can be recitified by changing any of the other relevant parameters. For instance, let's change sma to be large enough to account for this value of rpole and you'l...
b.set_value('sma@binary@component', 10)
2.3/tutorials/requiv.ipynb
phoebe-project/phoebe2-docs
gpl-3.0
These logger warnings are handy when running phoebe interactively, but in a script its also handy to be able to check whether the system is currently computable /before/ running run_compute. This can be done by calling run_checks which returns a boolean (whether the system passes all checks) and a message (a string des...
print(b.run_checks()) b.set_value('sma@binary@component', 5) print(b.run_checks())
2.3/tutorials/requiv.ipynb
phoebe-project/phoebe2-docs
gpl-3.0
CIFAR-10 Data Loading and Preprocessing
# Load the raw CIFAR-10 data. cifar10_dir = 'cs231n/datasets/cifar-10-batches-py' X_train, y_train, X_test, y_test = load_CIFAR10(cifar10_dir) # As a sanity check, we print out the size of the training and test data. print 'Training data shape: ', X_train.shape print 'Training labels shape: ', y_train.shape print 'Tes...
assignment1/svm.ipynb
srippa/nn_deep
mit
SVM Classifier Your code for this section will all be written inside cs231n/classifiers/linear_svm.py. As you can see, we have prefilled the function compute_loss_naive which uses for loops to evaluate the multiclass SVM loss function.
# Evaluate the naive implementation of the loss we provided for you: from cs231n.classifiers.linear_svm import svm_loss_naive import time # generate a random SVM weight matrix of small numbers W = np.random.randn(10, 3073) * 0.0001 loss, grad = svm_loss_naive(W, X_train, y_train, 0.00001) print 'loss: %f' % (loss, )
assignment1/svm.ipynb
srippa/nn_deep
mit
The grad returned from the function above is right now all zero. Derive and implement the gradient for the SVM cost function and implement it inline inside the function svm_loss_naive. You will find it helpful to interleave your new code inside the existing function. To check that you have correctly implemented the gra...
# Once you've implemented the gradient, recompute it with the code below # and gradient check it with the function we provided for you # Compute the loss and its gradient at W. loss, grad = svm_loss_naive(W, X_train, y_train, 0.0) # Numerically compute the gradient along several randomly chosen dimensions, and # comp...
assignment1/svm.ipynb
srippa/nn_deep
mit
Inline Question 1: It is possible that once in a while a dimension in the gradcheck will not match exactly. What could such a discrepancy be caused by? Is it a reason for concern? What is a simple example in one dimension where a gradient check could fail? Hint: the SVM loss function is not strictly speaking differenti...
# Next implement the function svm_loss_vectorized; for now only compute the loss; # we will implement the gradient in a moment. tic = time.time() loss_naive, grad_naive = svm_loss_naive(W, X_train, y_train, 0.00001) toc = time.time() print 'Naive loss: %e computed in %fs' % (loss_naive, toc - tic) from cs231n.classifi...
assignment1/svm.ipynb
srippa/nn_deep
mit
Stochastic Gradient Descent We now have vectorized and efficient expressions for the loss, the gradient and our gradient matches the numerical gradient. We are therefore ready to do SGD to minimize the loss.
# Now implement SGD in LinearSVM.train() function and run it with the code below from cs231n.classifiers import LinearSVM learning_rates = [1e-7, 5e-5] regularization_strengths = [5e4, 1e5] svm = LinearSVM() tic = time.time() loss_hist = svm.train(X_train, y_train, learning_rate=1e-5, reg=5e4, n...
assignment1/svm.ipynb
srippa/nn_deep
mit
Migrate evaluation <table class="tfo-notebook-buttons" align="left"> <td> <a target="_blank" href="https://www.tensorflow.org/guide/migrate/evaluator"> <img src="https://www.tensorflow.org/images/tf_logo_32px.png" /> View on TensorFlow.org</a> </td> <td> <a target="_blank" href="https://colab.rese...
import tensorflow.compat.v1 as tf1 import tensorflow as tf import numpy as np import tempfile import time import os mnist = tf.keras.datasets.mnist (x_train, y_train),(x_test, y_test) = mnist.load_data() x_train, x_test = x_train / 255.0, x_test / 255.0
site/en/guide/migrate/evaluator.ipynb
tensorflow/docs
apache-2.0
TensorFlow 1: Evaluating using tf.estimator.train_and_evaluate In TensorFlow 1, you can configure a tf.estimator to evaluate the estimator using tf.estimator.train_and_evaluate. In this example, start by defining the tf.estimator.Estimator and speciyfing training and evaluation specifications:
feature_columns = [tf1.feature_column.numeric_column("x", shape=[28, 28])] classifier = tf1.estimator.DNNClassifier( feature_columns=feature_columns, hidden_units=[256, 32], optimizer=tf1.train.AdamOptimizer(0.001), n_classes=10, dropout=0.2 ) train_input_fn = tf1.estimator.inputs.numpy_input_fn( ...
site/en/guide/migrate/evaluator.ipynb
tensorflow/docs
apache-2.0
Then, train and evaluate the model. The evaluation runs synchronously between training because it's limited as a local run in this notebook and alternates between training and evaluation. However, if the estimator is used distributedly, the evaluator will run as a dedicated evaluator task. For more information, check t...
tf1.estimator.train_and_evaluate(estimator=classifier, train_spec=train_spec, eval_spec=eval_spec)
site/en/guide/migrate/evaluator.ipynb
tensorflow/docs
apache-2.0
TensorFlow 2: Evaluating a Keras model In TensorFlow 2, if you use the Keras Model.fit API for training, you can evaluate the model with tf.keras.utils.SidecarEvaluator. You can also visualize the evaluation metrics in TensorBoard which is not shown in this guide. To help demonstrate this, let's first start by defining...
def create_model(): return tf.keras.models.Sequential([ tf.keras.layers.Flatten(input_shape=(28, 28)), tf.keras.layers.Dense(512, activation='relu'), tf.keras.layers.Dropout(0.2), tf.keras.layers.Dense(10) ]) loss = tf.keras.losses.SparseCategoricalCrossentropy(from_logits=True) model = create_mod...
site/en/guide/migrate/evaluator.ipynb
tensorflow/docs
apache-2.0
Then, evaluate the model using tf.keras.utils.SidecarEvaluator. In real training, it's recommended to use a separate job to conduct the evaluation to free up worker resources for training.
data = tf.data.Dataset.from_tensor_slices((x_test, y_test)) data = data.batch(64) tf.keras.utils.SidecarEvaluator( model=model, data=data, checkpoint_dir=log_dir, max_evaluations=1 ).start()
site/en/guide/migrate/evaluator.ipynb
tensorflow/docs
apache-2.0
$$ \pot_\cur = \sum_\prev \wcur \sigout\prev $$ $$ \sigout\cur = \activfunc(\pot_\cur) $$ $$ \weights = \begin{pmatrix} \weight_{11} & \cdots & \weight_{1m} \ \vdots & \ddots & \vdots \ \weight_{n1} & \cdots & \weight_{nm} \end{pmatrix} $$ Divers Le PMC peut approximer n'importe quelle fonction ...
%matplotlib inline import nnfigs # https://github.com/jeremiedecock/neural-network-figures.git import nnfigs.core as nnfig import matplotlib.pyplot as plt fig, ax = nnfig.init_figure(size_x=8, size_y=4) nnfig.draw_synapse(ax, (0, -6), (10, 0)) nnfig.draw_synapse(ax, (0, -2), (10, 0)) nnfig.draw_synapse(ax, (0, 2), ...
ai_ml_multilayer_perceptron_fr.ipynb
jdhp-docs/python-notebooks
mit
Apprentissage Mise à jours des poids $$ \weights(\learnit + 1) = \weights(\learnit) \underbrace{- \learnrate \nabla \errfunc \left( \weights(\learnit) \right)} $$ $- \learnrate \nabla \errfunc \left( \weights(\learnit) \right)$: descend dans la direction opposée au gradient (plus forte pente) avec $\nabla \errfunc \lef...
fig, ax = plt.subplots(nrows=1, ncols=1, figsize=(4, 4)) x = np.arange(10, 30, 0.1) y = (x - 20)**2 + 2 ax.set_xlabel(r"Poids $" + STR_WEIGHTS + "$", fontsize=14) ax.set_ylabel(r"Fonction objectif $" + STR_ERRFUNC + "$", fontsize=14) # See http://matplotlib.org/api/axes_api.html#matplotlib.axes.Axes.tick_params ax.t...
ai_ml_multilayer_perceptron_fr.ipynb
jdhp-docs/python-notebooks
mit
Apprentissage incrémentiel (ou partiel) (ang. incremental learning): on ajuste les poids $\weights$ après la présentation d'un seul exemple ("ce n'est pas une véritable descente de gradient"). C'est mieux pour éviter les minimums locaux, surtout si les exemples sont mélangés au début de chaque itération Apprentissage d...
%matplotlib inline import nnfigs # https://github.com/jeremiedecock/neural-network-figures.git import nnfigs.core as nnfig import matplotlib.pyplot as plt fig, ax = nnfig.init_figure(size_x=8, size_y=4) nnfig.draw_synapse(ax, (0, -2), (10, 0)) nnfig.draw_synapse(ax, (0, 2), (10, 0), label=tex(STR_WEIGHT + "_{" + S...
ai_ml_multilayer_perceptron_fr.ipynb
jdhp-docs/python-notebooks
mit
Plus de détail : calcul de $\errsig_\cur$ Dans l'exemple suivant on ne s'intéresse qu'aux poids $\weight_1$, $\weight_2$, $\weight_3$, $\weight_4$ et $\weight_5$ pour simplifier la demonstration.
%matplotlib inline import nnfigs # https://github.com/jeremiedecock/neural-network-figures.git import nnfigs.core as nnfig import matplotlib.pyplot as plt fig, ax = nnfig.init_figure(size_x=8, size_y=4) HSPACE = 6 VSPACE = 4 # Synapse ##################################### # Layer 1-2 nnfig.draw_synapse(ax, (0, V...
ai_ml_multilayer_perceptron_fr.ipynb
jdhp-docs/python-notebooks
mit
Attention: $\weight_1$ influe $\pot_2$ et $\pot_3$ en plus de $\pot_1$ et $\pot_o$. Calcul de $\frac{\partial \errfunc}{\partial \weight_4}$ rappel: $$ \begin{align} \errfunc &= \frac12 \left( \sigout_o - \sigoutdes_o \right)^2 \tag{1} \ \sigout_o &= \activfunc(\pot_o) \tag{2} \ \pot_o &= \sigout_2 \weight_4 + \si...
def sigmoid(x, _lambda=1.): y = 1. / (1. + np.exp(-_lambda * x)) return y %matplotlib inline x = np.linspace(-5, 5, 300) y1 = sigmoid(x, 1.) y2 = sigmoid(x, 5.) y3 = sigmoid(x, 0.5) plt.plot(x, y1, label=r"$\lambda=1$") plt.plot(x, y2, label=r"$\lambda=5$") plt.plot(x, y3, label=r"$\lambda=0.5$") plt.hline...
ai_ml_multilayer_perceptron_fr.ipynb
jdhp-docs/python-notebooks
mit
Fonction dérivée : $$ f'(x) = \frac{\lambda e^{-\lambda x}}{(1+e^{-\lambda x})^{2}} $$ qui peut aussi être défini par $$ \frac{\mathrm{d} y}{\mathrm{d} x} = \lambda y (1-y) $$ où $y$ varie de 0 à 1.
def d_sigmoid(x, _lambda=1.): e = np.exp(-_lambda * x) y = _lambda * e / np.power(1 + e, 2) return y %matplotlib inline x = np.linspace(-5, 5, 300) y1 = d_sigmoid(x, 1.) y2 = d_sigmoid(x, 5.) y3 = d_sigmoid(x, 0.5) plt.plot(x, y1, label=r"$\lambda=1$") plt.plot(x, y2, label=r"$\lambda=5$") plt.plot(x, y...
ai_ml_multilayer_perceptron_fr.ipynb
jdhp-docs/python-notebooks
mit
Tangente hyperbolique
def tanh(x): y = np.tanh(x) return y x = np.linspace(-5, 5, 300) y = tanh(x) plt.plot(x, y) plt.hlines(y=0, xmin=-5, xmax=5, color='gray', linestyles='dotted') plt.vlines(x=0, ymin=-2, ymax=2, color='gray', linestyles='dotted') plt.title("Fonction tangente hyperbolique") plt.axis([-5, 5, -2, 2]);
ai_ml_multilayer_perceptron_fr.ipynb
jdhp-docs/python-notebooks
mit
Dérivée : $$ \tanh '= \frac{1}{\cosh^{2}} = 1-\tanh^{2} $$
def d_tanh(x): y = 1. - np.power(np.tanh(x), 2) return y x = np.linspace(-5, 5, 300) y = d_tanh(x) plt.plot(x, y) plt.hlines(y=0, xmin=-5, xmax=5, color='gray', linestyles='dotted') plt.vlines(x=0, ymin=-2, ymax=2, color='gray', linestyles='dotted') plt.title("Fonction dérivée de la tangente hyperbolique") ...
ai_ml_multilayer_perceptron_fr.ipynb
jdhp-docs/python-notebooks
mit
Fonction logistique Fonctions ayant pour expression $$ f(t) = K \frac{1}{1+ae^{-rt}} $$ où $K$ et $r$ sont des réels positifs et $a$ un réel quelconque. Les fonctions sigmoïdes sont un cas particulier de fonctions logistique avec $a > 0$. Python implementation
# Define the activation function and its derivative activation_function = tanh d_activation_function = d_tanh def init_weights(num_input_cells, num_output_cells, num_cell_per_hidden_layer, num_hidden_layers=1): """ The returned `weights` object is a list of weight matrices, where weight matrix at index $i$...
ai_ml_multilayer_perceptron_fr.ipynb
jdhp-docs/python-notebooks
mit
ProPublica Campaign Finance API https://propublica.github.io/campaign-finance-api-docs/#candidates
# set key key="xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx" # set base url base_url="https://api.propublica.org/campaign-finance/v1/" # set headers headers = {'X-API-Key': key} # set url parameters cycle = "2014/" method = "candidates/" file_format = ".json" # create a list of FEC IDs from http://www.fec.gov/data/DataCata...
01_collect-data.ipynb
mathias-gibson/ps239t-final-project
mit
ProPublica Congress API - list of all members https://propublica.github.io/congress-api-docs/?shell#lists-of-members
# set key key="xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx" # set base url base_url="https://api.propublica.org/congress/v1/" # set url parameters congress = "114/" #102-114 for House, 80-114 for Senate chamber = "senate" #house or senate method="/members" file_format = ".json" #set headers headers = {'X-API-Key': key} #...
01_collect-data.ipynb
mathias-gibson/ps239t-final-project
mit
Create a grid with random elevation, set boundary conditions, and initialize components.
mg = RasterModelGrid((40, 40), 100) z = mg.add_zeros('topographic__elevation', at='node') z += np.random.rand(z.size) outlet_id = int(mg.number_of_node_columns * 0.5) mg.set_watershed_boundary_condition_outlet_id(outlet_id, z) mg.at_node['topographic__elevation'][outlet_id] = 0 fr = FlowAccumulator(mg) sp = Fastscape...
notebooks/tutorials/plotting/animate-landlab-output.ipynb
amandersillinois/landlab
mit
Set model time and uplift parameters.
simulation_duration = 1e6 dt = 1000 n_timesteps = int(simulation_duration // dt) + 1 timesteps = np.linspace(0, simulation_duration, n_timesteps) uplift_rate = 0.001 uplift_per_timestep = uplift_rate * dt
notebooks/tutorials/plotting/animate-landlab-output.ipynb
amandersillinois/landlab
mit
Phase 1: Animate elevation change using imshow_grid We first prepare the animation movie file. The model is run and the animation frames are captured together.
# Create a matplotlib figure for the animation. fig, ax = plt.subplots(1, 1) # Initiate an animation writer using the matplotlib module, `animation`. # Set up to animate 6 frames per second (fps) writer = animation.FFMpegWriter(fps=6) # Setup the movie file. writer.setup(fig, 'first_phase.mp4') for t in timesteps: ...
notebooks/tutorials/plotting/animate-landlab-output.ipynb
amandersillinois/landlab
mit
Finish the animation The method, writer.finish completes the processing of the movie and saves then it.
writer.finish()
notebooks/tutorials/plotting/animate-landlab-output.ipynb
amandersillinois/landlab
mit
This code loads the saved mp4 and presents it in a Jupyter Notebook.
HTML("""<div align="middle"> <video width="80%" controls loop> <source src="first_phase.mp4" type="video/mp4"> </video></div>""")
notebooks/tutorials/plotting/animate-landlab-output.ipynb
amandersillinois/landlab
mit
Phase 2: Animate multiple visualizations of elevation change over time In the second model phase, we will create an animation similar to the one above, although with the following differences: * The uplift rate is greater. * The animation file format is gif. * The figure has two subplots. * The data of one of the subpl...
increased_uplift_per_timestep = 10 * uplift_per_timestep
notebooks/tutorials/plotting/animate-landlab-output.ipynb
amandersillinois/landlab
mit
Run the second phase of the model Here we layout the figure with a left and right subplot. * The left subplot will be an animation of the grid similar to phase 1. We will recreate the image of this subplot for each animation frame. * The right subplot will be a line plot of the mean elevation over time. We will layout ...
# Create a matplotlib figure for the animation. fig2, axes = plt.subplots(1, 2, figsize=(9, 3)) fig2.subplots_adjust(top=0.85, bottom=0.25, wspace=0.4) # Layout right subplot. time = 0 line, = axes[1].plot(time, z.mean(), 'k') axes[1].set_title('mean elevation over time') axes[1].set_xlim([0, 1000]) axes[1].set_yli...
notebooks/tutorials/plotting/animate-landlab-output.ipynb
amandersillinois/landlab
mit
This code loads the saved mp4 and presents it in a Jupyter Notebook.
Image(filename='second_phase.gif')
notebooks/tutorials/plotting/animate-landlab-output.ipynb
amandersillinois/landlab
mit
Build up sensor to pvoutput model
from datetime import datetime,timedelta, time import pandas as pd import numpy as np import matplotlib.pyplot as plt from data_helper_functions import * from IPython.display import display pd.options.display.max_columns = 999 %matplotlib inline #iterate over datetimes: mytime = datetime(2014, 4, 1, 13) times = make_ti...
.ipynb_checkpoints/all-datasets-together-checkpoint.ipynb
scottlittle/solar-sensors
apache-2.0
...finally ready to model! Random Forest
from sklearn.cross_validation import train_test_split X_train, X_test, y_train, y_test = train_test_split(X, y, test_size=0.3, random_state=99) from sklearn.ensemble import RandomForestRegressor rfr = RandomForestRegressor(oob_score = True) rfr.fit(X_train,y_train) y_pred = rfr.predict(X_test) rfr.score(X_test,y_te...
.ipynb_checkpoints/all-datasets-together-checkpoint.ipynb
scottlittle/solar-sensors
apache-2.0
Linear model
#now do a linear model and compare: from sklearn.linear_model import LinearRegression lr = LinearRegression() lr.fit(X_train,y_train) lr.score(X_test,y_test) sorted_mask = np.argsort(lr.coef_) for i in zip(df_sensor.columns.values,lr.coef_[sorted_mask])[::-1]: print i df_sensor.ix[:,-15:-1].head() #selects photo...
.ipynb_checkpoints/all-datasets-together-checkpoint.ipynb
scottlittle/solar-sensors
apache-2.0
When only keeping the photometer data, random forest and linear model do pretty similar. When I added all of the sensor instruments to the fit, rfr scored 0.87 and lr scored negative! Also, I threw away the mysterious "Research 2" sensor, that was probably just a solar panel! I asked NREL what it is, so we'll see. I...
import pandas as pd import numpy as np from sklearn.preprocessing import scale from lasagne import layers from lasagne.nonlinearities import softmax, rectify, sigmoid, linear, very_leaky_rectify, tanh from lasagne.updates import nesterov_momentum, adagrad, momentum from nolearn.lasagne import NeuralNet import theano f...
.ipynb_checkpoints/all-datasets-together-checkpoint.ipynb
scottlittle/solar-sensors
apache-2.0
Extra Trees!
from sklearn.ensemble import ExtraTreesRegressor etr = ExtraTreesRegressor(oob_score=True, bootstrap=True, n_jobs=-1, n_estimators=1000) #nj_obs uses all cores! X_train, X_test, y_train, y_test = train_test_split(X, y, test_size=0.2, random_state=99) etr.fit(X_train, y_train) print etr.score...
.ipynb_checkpoints/all-datasets-together-checkpoint.ipynb
scottlittle/solar-sensors
apache-2.0
Save this thing and try it out on the simulated sensors!
from sklearn.externals import joblib joblib.dump(etr, 'data/sensor-to-power-model/sensor-to-power-model.pkl') np.savez_compressed('data/y.npz',y=y) #save y
.ipynb_checkpoints/all-datasets-together-checkpoint.ipynb
scottlittle/solar-sensors
apache-2.0
I have cloned the $\delta$a$\delta$i repository into '/home/claudius/Downloads/dadi' and have compiled the code. Now I need to add that directory to the PYTHONPATH variable:
sys.path.insert(0, '/home/claudius/Downloads/dadi') sys.path
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Now, I should be able to import $\delta$a$\delta$i
import dadi dir(dadi) import pylab %matplotlib inline x = pylab.linspace(0, 4*pylab.pi, 1000) pylab.plot(x, pylab.sin(x), '-r') %%sh # this allows me to execute a shell command ls
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
I have turned the 1D folded SFS's from realSFS into $\delta$d$\delta$i format by hand according to the description in section 3.1 of the manual. I have left out the masking line from the input file.
fs_ery = dadi.Spectrum.from_file('ERY.FOLDED.sfs.dadi_format') fs_ery
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
$\delta$a$\delta$i is detecting that the spectrum is folded (as given in the input file), but it is also automatically masking the 0th and 18th count category. This is a not a good behaviour.
# number of segregating sites fs_ery.data[1:].sum()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Single population statistics $\pi$
fs_ery.pi()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
I have next added a masking line to the input file, setting it to '1' for the first position, i. e. the 0-count category.
fs_ery = dadi.Spectrum.from_file('ERY.FOLDED.sfs.dadi_format', mask_corners=False)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
$\delta$a$\delta$i is issuing the following message when executing the above command: WARNING:Spectrum_mod:Creating Spectrum with data_folded = True, but mask is not True for all entries which are nonsensical for a folded Spectrum.
fs_ery
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
I do not understand this warning from $\delta$a$\delta$i. The 18-count category is sensical for a folded spectrum with even sample size, so should not be masked. Anyway, I do not understand why $\delta$a$\delta$i is so reluctant to keep all positions, including the non-variable one.
fs_ery.pi()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
The function that returns $\pi$ produces the same output with or without the last count category masked ?! I think that is because even if the last count class (966.62...) is masked, it is still included in the calculation of $\pi$. However, there is no obvious unmasking in the pi function. Strange! There are (at least...
# Calcualting pi with the formula from Wakeley2009 n = 36 # 36 sequences sampled from 18 diploid individuals pi_Wakeley = (sum( [i*(n-i)*fs_ery[i] for i in range(1, n/2+1)] ) * 2.0 / (n*(n-1)))/pylab.sum(fs_ery.data) # note fs_ery.data gets the whole fs_ery list, including masked entries pi_Wakeley
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
This is the value of $\pi_{site}$ that I calculated previously and included in the first draft of the thesis.
fs_ery.mask fs_ery.data # gets all data, including the masked one # Calculating pi with the formula from Gillespie: n = 18 p = pylab.arange(0, n+1)/float(n) p # Calculating pi with the formula from Gillespie: n / (n-1.0) * 2 * pylab.sum(fs_ery * p*(1-p))
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
This is the same as the output of dadi's pi function on the same SFS.
# the sample size (n) that dadi stores in this spectrum object and uses as n in the pi function fs_ery.sample_sizes[0] # what is the total number of sites in the spectrum pylab.sum(fs_ery.data)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
So, 1.6 million sites went into the ery spectrum.
# pi per site n / (n-1.0) * 2 * pylab.sum(fs_ery * p*(1-p)) / pylab.sum(fs_ery.data)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Apart from the incorrect small sample size correction by $\delta$a$\delta$i in case of folded spectra ($n$ refers to sampled sequences, not individuals), Gillespie's formula leads to a much higher estimate of $\pi_{site}$ than Wakeley's. Why is that?
# with correct small sample size correction 2 * n / (2* n-1.0) * 2 * pylab.sum(fs_ery * p*(1-p)) / pylab.sum(fs_ery.data) # Calculating pi with the formula from Gillespie: n = 18 p = pylab.arange(0, n+1)/float(n) p = p/2 # with a folded spectrum, we are summing over minor allele freqs only pi_Gillespie = 2*n / (2*n-...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
As can be seen from the insignificant difference (must be due to numerical inaccuracies) between the $\pi_{Wakeley}$ and the $\pi_{Gillespie}$ estimates, they are equivalent with the calculation for folded spectra given above as well as the correct small sample size correction. Beware: $\delta$a$\delta$i does not handl...
fs_ery.folded
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
I think for now it would be best to import unfolded spectra from realSFS and fold them if necessary in dadi.
fs_par = dadi.Spectrum.from_file('PAR.FOLDED.sfs.dadi_format') pylab.plot(fs_ery, 'r', label='ery') pylab.plot(fs_par, 'g', label='par') pylab.legend()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
ML estimate of $\theta$ from 1D folded spectrum I am trying to fit eq. 4.21 of Wakeley2009 to the oberseved 1D folded spectra. $$ E[\eta_i] = \theta \frac{\frac{1}{i} + \frac{1}{n-i}}{1+\delta_{i,n-i}} \qquad 1 \le i \le \big[n/2\big] $$ Each frequency class, $\eta_i$, provides an estimate of $\theta$. However, I would...
from scipy.optimize import least_squares def model(theta, eta, n): """ theta: scaled population mutation rate parameter [scalar] eta: the folded 1D spectrum, including 0-count cat. [list] n: number of sampled gene copies, i. e. 2*num_ind [scalar] returns a numpy array """ i = pylab.ar...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
The counts in each frequency class should be Poisson distributed with rate equal to $E[\eta_i]$ as given above. The lowest frequency class has the highest rate and therefore also the highest variance
#?plt.ylabel #print plt.rcParams fs_ery[1:].max() #?pylab os.getcwd() %%sh ls
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
The following function will take the file name of a file containing the flat 1D folded frequency spectrum of one population and plots it together with the best fitting neutral expectation.
def plot_folded_sfs(filename, n, pop = ''): # read in spectrum from file data = open(filename, 'r') sfs = pylab.array( data.readline().split(), dtype=float ) data.close() # should close connection to file #return sfs # get starting value for theta from Watterson's theta S = sfs[1:].sum(...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Univariate function minimizers or 1D scalar minimisation Since I only have one value to optimize, I can use a slightly simpler approach than used above:
from scipy.optimize import minimize_scalar ?minimize_scalar # define cost function def f(theta, eta, n): """ return sum of squared deviations between model and data """ return sum( (model(theta, eta, n) - eta[1:])**2 ) # see above for definition of the 'model' function
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
It would be interesting to know whether the cost function is convex or not.
theta = pylab.arange(0, fs_ery.data[1:].sum()) # specify range of theta cost = [f(t, fs_ery.data, 36) for t in theta] plt.plot(theta, cost, 'b-', label='ery') plt.xlabel(r'$\theta$') plt.ylabel('cost') plt.title("cost function for ery") plt.legend(loc='best') ?plt.legend
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Within the specified bounds (the observed $\theta$, i. e. derived from the data, cannot lie outside these bounds), the cost function is convex. This is therefore an easy optimisation problem. See here for more details.
res = minimize_scalar(f, bounds = (0, fs_ery.data[1:].sum()), method = 'bounded', args = (fs_ery.data, 36)) res # number of segregating sites fs_par.data[1:].sum() res = minimize_scalar(f, bounds = (0, fs_par.data[1:].sum()), method = 'bounded', args = (fs_par.data, 36)) res
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
The fitted values of $\theta$ are similar to the ones obtained above with the least_squares function. The estimates for ery deviate more than for par.
from sympy import * x0 , x1 = symbols('x0 x1') init_printing(use_unicode=True) diff(0.5*(1-x0)**2 + (x1-x0**2)**2, x0) diff(0.5*(1-x0)**2 + (x1-x0**2)**2, x1)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Wow! Sympy is a replacement for Mathematica. There is also Sage, which may include even more functionality.
from scipy.optimize import curve_fit
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Curve_fit is another function that can be used for optimization.
?curve_fit def model(i, theta): """ i: indpendent variable, here minor SNP frequency classes theta: scaled population mutation rate parameter [scalar] returns a numpy array """ n = len(i) delta = pylab.where(i == n-i, 1, 0) return theta * 1/i + 1/(n-i) / (1 + delta) i = pylab.aran...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
I am not sure whether these standard errors (perr) are correct. It may be that it is assumed that errors are normally distributed, which they are not exactly in this case. They should be close to Poisson distributed (see Fu1995), which should be fairly similar to normal with such high expected values as here. If the st...
%pwd % ll ! cat ERY.FOLDED.sfs.dadi_format fs_ery = dadi.Spectrum.from_file('ERY.FOLDED.sfs.dadi_format', mask_corners=False) fs_ery fs_ery.pop_ids = ['ery'] # get a Poisson sample from the observed spectrum fs_ery_param_boot = fs_ery.sample() fs_ery_param_boot fs_ery_param_boot.data %psource fs_ery.sample
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
There must be a way to get more than one bootstrap sample per call.
fs_ery_param_boot = pylab.array([fs_ery.sample() for i in range(100)]) # get the first 3 boostrap samples from the doubleton class fs_ery_param_boot[:3, 2]
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
It would be good to get the 5% and 95% quantiles from the bootstrap samples of each frequency class and add those intervals to the plot of the observed frequency spectrum and the fitted neutral spectrum. This would require to find a quantile function and to find out how to add lines to a plot with matplotlib. It would ...
# read in the flattened 2D SFS EryPar_unfolded_2dsfs = dadi.Spectrum.from_file('EryPar.unfolded.2dsfs.dadi_format', mask_corners=True) # check dimension len(EryPar_unfolded_2dsfs[0,]) EryPar_unfolded_2dsfs.sample_sizes # add population labels EryPar_unfolded_2dsfs.pop_ids = ["ery", "par"] EryPar_unfolded_2dsfs.pop_...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Marginalizing $\delta$a$\delta$i offers a function to get the marginal spectra from multidimensional spectra. Note, that this marginalisation is nothing fancy. In R it would be taking either the rowSums or the colSums of the matrix.
# marginalise over par to get 1D SFS for ery fs_ery = EryPar_unfolded_2dsfs.marginalize([1]) # note the argument is an array with dimensions, one can marginalise over more than one dimension at the same time, # but that is only interesting for 3-dimensional spectra, which I don't have here fs_ery # marginalise over...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Note, that these marginalised 1D SFS's are not identical to the 1D SFS estimated directly with realSFS. This is because, for the estimation of the 2D SFS, realSFS has only taken sites that had data from at least 9 individuals in each population (see assembly.sh, lines 1423 onwards). The SFS's of par and ery had conspic...
# plot 1D spectra for each population pylab.plot(fs_par, 'g', label="par") pylab.plot(fs_ery, 'r', label="ery") pylab.legend()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
These marginal unfolded spectra look similar in shape to the 1D folded spectra of each subspecies (see above).
fs_ery.pi() / pylab.sum(fs_ery.data) fs_ery.data n = 36 # 36 sequences sampled from 18 diploid individuals pi_Wakeley = (sum( [i*(n-i)*fs_ery[i] for i in range(1, n)] ) * 2.0 / (n*(n-1))) pi_Wakeley = pi_Wakeley / pylab.sum(fs_ery.data) pi_Wakeley
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
$\delta$a$\delta$i's pi function seems to calculate the correct value of $\pi$ for this unfolded spectrum. However, it is worrying that $\pi$ from this marginal spectrum is about 20 times larger than the one calculated from the directly estimated 1D folded spectrum (see above the $\pi$ calculated from the folded 1D spe...
fs_par.pi() / pylab.sum(fs_par.data) pylab.sum(fs_par.data) pylab.sum(EryPar_unfolded_2dsfs.data)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
<font color="red">The sum over the marginalised 1D spectra should be the same as the sum over the 2D spectrum !</font>
# from dadi's marginalise function: fs_ery.data sfs2d = EryPar_unfolded_2dsfs.copy() # this should get the marginal spectrum for ery ery_mar = [pylab.sum(sfs2d.data[i]) for i in range(0, len(sfs2d))] ery_mar # this should get the marginal spectrum for ery and then take the sum over it sum([pylab.sum(sfs2d.data[i]) f...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
So, during the marginalisation the masking of data in the fixed categories (0, 36) is the problem, producing incorrectly marginalised counts in those masked categories. This is shown in the following:
sfs2d[0] pylab.sum(sfs2d[0]) # from dadi's marginalise function: fs_ery.data # dividing by the correct number of sites to get pi per site: fs_ery.pi() / pylab.sum(sfs2d.data)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
This is very close to the estimate of $\pi$ derived from the folded 1D spectrum of ery! (see above)
fs_par.pi() / pylab.sum(sfs2d.data)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
This is also nicely close to the estimate of $\pi_{site}$ of par from its folded 1D spectrum. Tajima's D
fs_ery.Watterson_theta() / pylab.sum(sfs2d.data) fs_ery.Tajima_D() fs_par.Tajima_D()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Now, I am calculating Tajima's D from the ery marginal spectrum by hand in order to check whether $\delta$a$\delta$i is doing the right thing.
n = 36 pi_Wakeley = (sum( [i*(n-i)*fs_ery.data[i] for i in range(1, n+1)] ) * 2.0 / (n*(n-1))) #/ pylab.sum(sfs2d.data) pi_Wakeley # number of segregating sites # this sums over all unmasked positions in the array pylab.sum(fs_ery) fs_ery.S() S = pylab.sum(fs_ery) theta_Watterson = S /...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
$\delta$a$\delta$i seems to do the right thing. Note, that the estimate of Tajima's D from this marginal spectrum of ery is slightly different from the estimate derived from the folded 1D spectrum of ery (see /data3/claudius/Big_Data/ANGSD/SFS/SFS.Rmd). The folded 1D spectrum resulted in a Tajima's D estimate of $\sim$...
fs_par.Tajima_D()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
My estimate from the folded 1D spectrum of par was -0.6142268 (see /data3/claudius/Big_Data/ANGSD/SFS/SFS.Rmd). Multi-population statistics
EryPar_unfolded_2dsfs.S()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
The 2D spectrum contains counts from 60k sites that are variable in par or ery or both.
EryPar_unfolded_2dsfs.Fst()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
This estimate of $F_{ST}$ according to Weir and Cockerham (1984) is well below the estimate of $\sim$0.3 from ANGSD according to Bhatia/Hudson (2013). Note, however, that this estimate showed a positive bias of around 0.025 in 100 permutations of population labels of individuals. Taking the positive bias into account, ...
%psource EryPar_unfolded_2dsfs.scramble_pop_ids # plot the scrambled 2D SFS dadi.Plotting.plot_single_2d_sfs(EryPar_unfolded_2dsfs.scramble_pop_ids(), vmin=1)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
So, this is how the 2D SFS would look like if ery and par were not genetically differentiated.
# get Fst for scrambled SFS EryPar_unfolded_2dsfs.scramble_pop_ids().Fst()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
The $F_{ST}$ from the scrambled SFS is much lower than the $F_{ST}$ of the observed SFS. That should mean that there is significant population structure. However, the $F_{ST}$ from the scrambled SFS is not 0. I don't know why that is.
# folding EryPar_folded_2dsfs = EryPar_unfolded_2dsfs.fold() EryPar_folded_2dsfs EryPar_folded_2dsfs.mask
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Plotting
dadi.Plotting.plot_single_2d_sfs(EryPar_unfolded_2dsfs, vmin=1) dadi.Plotting.plot_single_2d_sfs(EryPar_folded_2dsfs, vmin=1)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
The folded 2D spectrum is not a minor allele frequency spectrum as are the 1D folded spectra of ery and par. This is because an allele that is minor in one population can be the major allele in the other. What is not counted are the alleles that are major in both populations, i. e. the upper right corner. For the 2D sp...
# unfolded spectrum from marginalisation of 2D unfolded spectrum fs_ery len(fs_ery) fs_ery.fold()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Let's use the formula (1.2) from Wakeley2009 to fold the 1D spectrum manually: $$ \eta_{i} = \frac{\zeta_{i} + \zeta_{n-i}}{1 + \delta_{i, n-i}} \qquad 1 \le i \le [n/2] $$ $n$ is the number of gene copies sampled, i. e. haploid sample size. $[n/2]$ is the largest integer less than or equal to n/2 (to handle uneven sam...
fs_ery_folded = fs_ery.copy() # make a copy of the UNfolded spectrum n = len(fs_ery)-1 for i in range(len(fs_ery)): fs_ery_folded[i] += fs_ery[n-i] if i == n/2.0: fs_ery_folded[i] /= 2 fs_ery_folded[0:19] isinstance(fs_ery_folded, pylab.ndarray) mask = [True] mask.extend([False] * 18) mask.extend([Tr...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Here is how to flatten an array of arrays with list comprehension:
mask = [[True], [False] * 18, [True] * 18] print mask print [elem for a in mask for elem in a]
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Set new mask for the folded spectrum:
fs_ery_folded.mask = mask fs_ery_folded.folded = True fs_ery_folded - fs_ery.fold()
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
The fold() function works correctly for 1D spectra, at least. How about 2D spectra? $$ \eta_{i,j} = \frac{\zeta_{i,j} + \zeta_{n-i, m-j}}{1 + \delta_{i, n-i; j, m-j}} \qquad 1 \le i+j \le \Big[\frac{n+m}{2}\Big] $$
EryPar_unfolded_2dsfs.sample_sizes EryPar_unfolded_2dsfs._total_per_entry() # copy the unfolded 2D spectrum for folding import copy sfs2d_folded = copy.deepcopy(EryPar_unfolded_2dsfs) n = len(sfs2d_folded)-1 m = len(sfs2d_folded[0])-1 for i in range(n+1): for j in range(m+1): sfs2d_folded[i,j] += sfs2d_f...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
I am going to go through every step in the fold function of dadi:
# copy the unfolded 2D spectrum for folding import copy sfs2d_unfolded = copy.deepcopy(EryPar_unfolded_2dsfs) total_samples = pylab.sum(sfs2d_unfolded.sample_sizes) total_samples total_per_entry = dadi.Spectrum(sfs2d_unfolded._total_per_entry(), pop_ids=['ery', 'par']) #total_per_entry.pop_ids = ['ery', 'par'] dadi.P...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
With the variable length list of slice objects, one can generalise the reverse of arrays with any dimensions.
final_mask = pylab.logical_or(original_mask, dadi.Numerics.reverse_array(original_mask)) final_mask
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
Here, folding doesn't mask new cells.
?pylab.where pylab.where(matrix < 6, matrix, 0) # this takes the part of the spectrum that is non-sensical if the derived allele is not known # and sets the rest to 0 print pylab.where(where_folded_out, sfs2d_unfolded, 0) # let's plot the bit of the spectrum that we are going to fold onto the rest: dadi.Plotting.plo...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
The transformation we have done with the upper-right diagonal 2D array above should be identical to projecting it across a vertical center line (creating an upper left triangular matrix) and then projecting it across a horizontal center line (creating the final lower left triangular matrix). Note, that this is not like...
# This shall now be added to the original unfolded 2D spectrum. sfs2d_folded = pylab.ma.masked_array(sfs2d_unfolded.data + _reversed) dadi.Plotting.plot_single_2d_sfs(dadi.Spectrum(sfs2d_folded), vmin=1) sfs2d_folded.data sfs2d_folded.data[where_folded_out] = 0 sfs2d_folded.data dadi.Plotting.plot_single_2d_sfs(da...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
SNP's with joint frequencies in the True cells are counted twice at the moment due to the folding and the fact that the sample sizes are even.
# this extracts the diagonal values from the UNfolded spectrum and sets the rest to 0 ambiguous = pylab.where(where_ambiguous, sfs2d_unfolded, 0) dadi.Plotting.plot_single_2d_sfs(dadi.Spectrum(ambiguous), vmin=1)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
These are the values in the diagonal before folding.
reversed_ambiguous = dadi.Numerics.reverse_array(ambiguous) dadi.Plotting.plot_single_2d_sfs(dadi.Spectrum(reversed_ambiguous), vmin=1)
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit
These are the values that got added to the diagonal during folding. Comparing with the previous plot, one can see for instance that the value in the (0, 36) class got added to the value in the (36, 0) class and vice versa. The two frequency classes are equivalent, since it is arbitrary which allele we call minor in the...
a = -1.0*ambiguous + 0.5*ambiguous + 0.5*reversed_ambiguous b = -0.5*ambiguous + 0.5*reversed_ambiguous a == b sfs2d_folded += -0.5*ambiguous + 0.5*reversed_ambiguous final_mask = pylab.logical_or(final_mask, where_folded_out) final_mask sfs2d_folded = dadi.Spectrum(sfs2d_folded, mask=final_mask, data_folded=True, p...
Data_analysis/SNP-indel-calling/dadi/dadiExercises/First_Steps_with_dadi.ipynb
claudiuskerth/PhDthesis
mit