license stringclasses 1
value | text stringlengths 66 12.2k | doi stringlengths 10 59 | type stringclasses 14
values | detected_lang stringclasses 6
values | publication_year float64 2.01k 2.02k | is_dataset bool 2
classes | is_software bool 2
classes | is_acknowledgement bool 2
classes | is_clinicaltrial bool 2
classes | field_name stringclasses 26
values | field_id stringclasses 26
values |
|---|---|---|---|---|---|---|---|---|---|---|---|
cc-by | Interoperability is a topic which has already been discussed and studied at length over multiple large research projects. The CLARIN Project (19) produced a review of accepted standards which were designed to promote interoperability in multiple fields. The META-NET project (112) produced a language resources sharing... | 10.1093/database/baw145 | review | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Finally, both the annotated corpus and the term inventory encoded in OBO format (see 'Formats for knowledge resources' section) were published in the META-SHARE repository (see 'Language resources repositories' section). Employing open standards, formats and services for publishing annotated content, created vocabulari... | 10.1093/database/baw145 | review | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Funding This work is jointly supported by the EC/H2020 project: an Open Mining INfrastructure for TExt and Data (OpenMinTeD) Grant ID: 654021 and the BBSRC project: Enriching Metabolic PATHwaY models with evidence from the literature (EMPATHY) Grant ID: BB/M006891/1. | 10.1093/database/baw145 | review | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Molecular biology We used pDG1730 (Genbank U46199, (37) ) that we obtained from the Bacillus Genetic Stock Center (http://www. bgsc. org) as our backbone plasmid for B. subtilis integration into the amyE locus. All plasmids used in this study were derived from this vector and fragments assembled using onestep isotherm... | 10.1093/nar/gkw624 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Voltages used were FFS: 440, SSC: 340, BL1: 490, YL2: 620. Data were analysed using the Attune NxT software. Flow-Jo (Tristar) was used for data representation. Statistical values for each channels of the sample were calculated and exported. For each independent experiment, the median fluorescence intensity of the bact... | 10.1093/nar/gkw624 | article | en | 2,016 | true | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | 485/20 nm, em. 528/20 nm) and absorbance (at 600 nm) every 10 min. Absorbance at 900 nm and 977 nm (Abs 900 , Abs 977 ) were read once at the beginning of each experiment in order to correct the OD 600 to an optical path length of 1 cm using the following equation: (Abs 977 -Abs 900 )/0. 18 (40). Fluorescein was prese... | 10.1093/nar/gkw624 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Individual cells in each FOV were contoured automatically with manual correction using the Patrack software (43). For each FOV, fluorescence fluctuations (δF) from the average intensity over 50 scans (<F>) were first calculated at each pixel, providing pixel-based maps of the true (shot noise corrected) molecular brig... | 10.1093/nar/gkw624 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Choice and characterisation of ten B. subtilis constitutive promoters We aimed at designing synthetic libraries of constitutive promoters spanning a wide dynamic range of transcriptional efficiencies in B. subtilis. Such constitutive promoter libraries are essential tools for precise engineering of genetic circuits. Fo... | 10.1093/nar/gkw624 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | ACKNOWLEDGEMENTS We thank Emmanuel Margeat , Luca Ciandrini , Joachim Rambeau , Guillaume Cambray , and members of the Bonnet and Jules lab for fruitful discussions. We acknowledge France-BioImaging infrastructure supported by the French National Research Agency ( ANR-10-INSB-04-01 , Investments for the futur... | 10.1093/nar/gkw624 | article | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Methods All experiments were performed at room temperature (RT). Experimental buffer was Hepes 10 mM, pH = 7. 4, KCl 150 mM, EDTA 2 mM except for QCM-D binding experiments. All images were acquired with a Zeiss LSM 780 microscope (Carl Zeiss, Inc. ) using a 63x NA 1. 4 oil objective and quantified using Image J softwar... | 10.1038/srep39332 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | 07 to 2250 μM in 100 μl of the same buffer that the one used in quenching experiments, according to the protocol in ref. 53. After 15 min of incubation at room temperature, samples were centrifuged at 220,000 g for 1 h at 4 °C using a Beckman Coulter's TLA 100 rotor. The top 80 μl was considered as supeRNAtant (S) and ... | 10.1038/srep39332 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgements We are thankful to Dr. Olivier Coux for his help with protein purification and Dr. Michel Franco for the gift of PH-EFA6 plasmid. We thank the Montpellier RIO Imaging (MRI) microscopy facilities. This work has been funded by ANR ( ANR-13-BSV5-0006-01 ). C. P. acknowledges the European Commissi... | 10.1038/srep39332 | article | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | The main difficulty in measuring the relative angular positions of the successive blades is linked to the lack of rigidity of algae (as compared to plants). For this reason we used a small apparatus described in Peaucelle [30] in which the branch is attached at both ends and held vertically. The axis of the branch co... | 10.5586/asbp.3526 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Acknowledgments A. P. is grateful to Elliot Meyerowitz for support and discussions and the SLCU microscopy facility supported by the Gatsby foundation. Y. C. is grateful to Agnes Schermann from Université de Rennes for introducing him to the complex world of algae during a CNRS summer school held in Berder and ... | 10.5586/asbp.3526 | article | en | 2,016 | false | false | true | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | First cultivated in Greece [5] , sweet cherry was later spread all over Europe. Its cultivation seems to be very old, its grafting technique was already described by the Roman writer Varo BC, and Pliny (23-79 AD) gave information of eight distinct cultivars [6, 7]. As a result of centuries of natural and human select... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | SNPs genotyping All accessions were genotyped using the RosBREED cherry 6 K Illumina Infinium II® SNP array v1 [24]. Genotype differences were recorded in the iSCAN platform and SNP genotypes were determined using Genome Studio Genotyping Module (Version 1. 8. 4, Illumina™) as described in [24]. The RosBREED cherry 6... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | SNP positions of the ROSBREED cherry 6 K array v1. 0 on the peach genome v2. 0 were redefined using batch BLAST function available at the GDR's website (GDR; www. rosaceae. org [26] ) (Additional file 1: Table S2 ). Illumina's GenCall software algorithms for clustering, calling and scoring genotypes were first used to... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Linkage disequilibrium Because LD can affect both Principal Coordinate Analysis (PCoA) and STRUCTURE analysis, the marker set was pruned by excluding SNPs in strong LD using PLINK software [30]. SNPs were pruned with a window of 50 SNPs and a step size of 5 makers. The r 2 threshold was 0. 5. Pairwise LD measures for ... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Population structure PCoA (also referred to as Classical Multidimensional Scaling), Bayesian-based (STRUCTURE software [38] ) and Discriminant Analysis of Principal Components (DAPC) analysis were used to investigate the pattern of population structure. PCoA is a distance-based model which uses jointly a dissimilarity... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | [38] and the ΔK method, described by Evanno et al. [37] and implemented in Structure Harvester [41] , were used. In order to assess assignment success, STRUCTURE was run by enforcing K to its true value. For a given K, we used the run that had the highest likelihood estimate to assign cluster proportions to individu... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Pairwise F st [42] among the subpopulations identified by STRUC-TURE were calculated using adegenet 2. 0. The assumptions underlying the population genetics model in STRUCTURE may limit its use in crops. Unlike natural populations, crops are subjected to displacements, breeding, clonal propagation, absence of panmict... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | This multivariate method is suitable for analyzing large numbers of genome-wide SNPs, and it provides individuals' assignment to groups as well as a visual assessment of between-population differentiation. The number of PCs retained can have a substantial impact on the results of the analysis. Indeed, retaining too man... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Population structure The genetic structure of the INRA's Sweet Cherry genetic resources collection was analyzed using STRUCTURE, PCoA and DAPC. All analyses were performed with the LD-pruned 326 SNP set. Thanks to the scalability of STRUCTURE software and MCIA multi-core infrastructure, we reduced the computing time fr... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | The landraces cluster was more scattered than the breeding cultivar one. The admixed accessions were dispersed between these two clusters along the axis 2 (Fig. 3 ). Pairwise F st values among STRUCTURE clusters ranged from 0. 022 (Admixed-Bred cultivars) to 0. 058 (Landraces-Bred cultivars) (Additional file 1: Table ... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | The functions 'find. clusters' and 'k-means' algorithm were used to determine the number of clusters maximizing the variation between clusters [31]. To avoid the loss of Fig. 2 Inferred population structure of the collection using STRUCTURE software. Bar plot of individual ancestry proportions for the genetic cluster... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | The Bayesian Information Criterion (BIC) was used to identify the optimal number of clusters, 9, indicated by an elbow curve of BIC values as a function of k (Additional file 2: Figure S6 ). The number of retained PC for DAPC analyses was calculated using a cross validation method implemented in 'xvalDapc' function fr... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | ), 47 % of the American ones in particular 'Hardy Giant' and 'Garnet', and 61 % of the French ones, with 'Fercer' and all its derived hybrids ('Ferprime', 'Ferdiva', 'Ferdouce', 'Feria'), except 'Folfer' and 'Ferlizac' which are included in DAPC clusters 3 and 5, respectively. Most of the accessions comprised in cluste... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | true | false | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Our results are in agreement with a previous structuration of sweet cherry cultivars into landraces and modern varieties [11]. We completed the analysis using the maximum likelihood parameter as recommended by Pritchard et al. [30] , in that case K was set to nine. This value of K appeared to fit with the origin and t... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Also DAPC analysis provided a more detailed clustering within landraces and bred cultivars compared STRUCTURE analysis either in our study or in previous analysis using SSR [11]. Regarding membership to clusters, DAPC provides membership values that are different from admixture coefficients from STRUCTURE, but they ca... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | true | false | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Acknowledgements We thank French ministry for agriculture and food (MAAP n°C-2011- 05 project) for financing part of this work. We thank INRA, Aquitaine Region and CEP Innovation ( AQUIPRU project 2014-1R20102-2971 ) for financing doctoral and postdoctoral fellowships to RB and JAC, respectively. We thank Dr Ange... | 10.1186/s12870-016-0712-9 | article | en | 2,016 | false | false | true | false | Agricultural and Biological Sciences | https://openalex.org/fields/11 |
cc-by | Whole mount in situ hybridization In situ hybridization was carried out as previously described [36] with several modifications as previously specified [32]. Templates for the PRIle-piwi1, PRIle-vasa, and PRIle-nanos probes were, respectively, ~1900 bp (positions 990-2968 in GenBank KM078049), ~2250 bp (positions 22... | 10.1186/s13227-016-0059-1 | article | en | 2,016 | true | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Gonads regress upon starvation and regrow upon refeeding We investigated whether the gonads of P. leidyi are sensitive to feeding regime by starving and refeeding worms over several weeks and assessing gonad expression of three germline/multipotency genes, homologs of piwi, nanos, and vasa ("Methods, " Experiment 1). W... | 10.1186/s13227-016-0059-1 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgements We thank Ryan Null and members of the Bely Lab for helpful discussions and comments on this project and this paper. | 10.1186/s13227-016-0059-1 | article | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Changes in conductivity can be used to evaluate the cmc of surfactants. In Fig. 2b , the conductivity of 2NapFF solutions is shown as a function of concentration. A detailed analysis of this measurement shows a significant reduction in the rate of increase in conductivity in the concentration range from 0. 008 to 0. 04... | 10.1039/c5sm03072b | article | en | 2,016 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | 33 Our conductivity data suggest that for 2NapFF, there is a first cmc (cmc 1 ) in the region from 0. 008 to 0. 02 wt% (region I shown in Fig. 2 ). From 0. 05 to 0. 1 wt%, there is a significant increase in the conductivity with a peak in the data at 0. 07 wt% (Fig. 3b ), suggesting a second transition within this conc... | 10.1039/c5sm03072b | article | en | 2,016 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | 3 wt% (Fig. S19 and S20, ESI †). However for concentrations below 0. 3 wt%, a distinct peak is visible at 1643 cm À1 in the hydrogel, which is not present in the solution. These IR data imply that there is possibly a molecular packing and structural transformation, at least between 0. 1 wt% and 0. 3 wt% when the solut... | 10.1039/c5sm03072b | article | en | 2,016 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Attempts were made with AFM, TEM, and SEM. As an Fig. 5 FTIR spectra of (a) 2NapFF solutions at high pD 11. 5 AE 0. 5 (0. 05 to 0. 5 wt%) and (b) the corresponding hydrogels (0. 05 to 0. 5 wt%) at the lower limits of detection. For the complete data set for the solution and gel phase, see Fig. S19 and S20 (ESI †). ex... | 10.1039/c5sm03072b | article | en | 2,016 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | S24b, ESI † ). However, a blank of a salt solution prepared under the same conditions (Fig. S24f , ESI †) shows similar spherical structures and hence we assume that the spheres imaged in the 0. 03 wt% gel, and most likely at other low concentrations (Fig. S24 , ESI †), are also from drying artifacts from the salt. As ... | 10.1039/c5sm03072b | article | en | 2,016 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | The samples were left to stand for a 24 hours incubation period at 22 1C. Surface tension. The surface tension measurements were performed on a high throughput Kibron Delta-8 Surface Tensiometer which uses a Du Nouy-Padday method (maximum pull on a rod). The results were analysed by the Delta-8 Manager software. The pr... | 10.1039/c5sm03072b | article | en | 2,016 | true | true | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | 001 M Nile blue in the initial solution (pipetting a selected amount from a 0. 01 M stock solution). Typical pictures selected from over the 50 captured were chosen and measured for this study. The images were analysed using the Zeiss AIM software (Zeiss, Jena, Germany) and ImageJ software. 3 ourier transform infrared ... | 10.1039/c5sm03072b | article | en | 2,016 | false | true | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | 001 to 0. 3 Å À1 , by using three sample-detector distances. The high flux available from the reactor source enabled measuring times of 30 minutes or less for each instrument setting. The data were reduced to 1D scattering curves of intensity vs. Q using the facility provided software. This involves the following key s... | 10.1039/c5sm03072b | article | en | 2,016 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | The instrument-independent data were then fitted to customised models in the SasView software package, 56 these combined an absolute power law with either a hollow cylinder 57 or a (Kratky-Porod) flexible cylinder. 58, 59 The Q-dependent power law (Q ÀN ) accounts for the mass fractal contribution to the scattering i... | 10.1039/c5sm03072b | article | en | 2,016 | false | true | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Acknowledgements AZC thanks the University of Liverpool for funding. DA thanks the EPSRC for a Fellowship ( EP/L021978/1 ). The experiment at the Institut Laue Langevin was allocated beam time under experiment number 9-10-1304. This work benefitted from the SasView software, originally developed by the DANSE p... | 10.1039/c5sm03072b | article | en | 2,016 | false | false | true | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Acknowledgements AZC thanks the University of Liverpool for funding. DA thanks the EPSRC for a Fellowship ( EP/L021978/1 ). The experiment at the Institut Laue Langevin was allocated beam time under experiment number 9-10-1304. This work benefitted from the SasView software, originally developed by the DANSE p... | 10.1039/c5sm03072b | article | en | 2,016 | false | false | true | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Rheological Studies The mechanical properties of hydrogels stabilized by bolaamphiphile 7 were characterized by rheological measurements. The storage (G ) and loss (G ) moduli provided information about the visco-elastic properties of gels. The frequency dependence of G and G moduli for the hydrogel stabilized by compo... | 10.3390/gels2040025 | article | en | 2,016 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Rheological Studies The mechanical properties of hydrogels stabilized by bolaamphiphile 7 were characterized by rheological measurements. The storage (G′) and loss (G′′) moduli provided information about the visco-elastic properties of gels. The frequency dependence of G′ and G′′ moduli for the hydrogel stabilized by c... | 10.3390/gels2040025 | article | en | 2,016 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | 22 g, 20 mmol) in anhydrous DMF (100 mL). After stirring for 24 h at 65 • C, the mixture was cooled to room temperature and poured into water (200 mL). The resulting solid was filtered off, washed with water, and dried under vacuum, with a crude yield of 6. 92 g (92%). NMR data were identical with those obtained from a... | 10.3390/gels2040025 | article | en | 2,016 | true | false | false | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Acknowledgments: The authors acknowledge financial supports from the " French National Research Program for Environmental and Occupational Health of ANSES " ( 2015/1/072 ). This work was realized within the framework of the Laboratory of Excellence AMADEus with the reference ANR-10-LABX-0042 AMADEUS. | 10.3390/gels2040025 | article | en | 2,016 | false | false | true | false | Materials Science | https://openalex.org/fields/25 |
cc-by | Acknowledgements This work was conducted in the framework of the IFREMER internal R&D project '' PJ0807 -Acoustique Sous-Marine ''. The publication of this work was suggested and encouraged by Pr. Larry Mayer ( UNH/CCOM, Durham, NH ), who brought many improvements to the manuscript. The measured directivity pattern... | 10.1016/j.apacoust.2015.07.012 | article | en | 2,015 | false | false | true | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Real-time quantitative PCR. Real-time quantitative PCR reactions were performed as previously described 39 (Supplementary Methods). Immunoblot analysis. Protein samples were dissolved in SDS sample buffer (Laemmli), heated to 96 °C for 5 min, and loaded onto a 4-15% Criterion TGX Stain-Free Precast gel (Bio-Rad). The... | 10.1038/ncomms10682 | article | en | 2,016 | false | true | false | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | The experimenter was blind to the construct used, which was revealed after analysis. For endogenous GluA2 QD tracking, rat hippocampal neurons were incubated with monoclonal antibody directed against N-terminal extracellular domain of GluA2 subunit for 10 min followed by 5-min incubation with QDs 655 Goat F(ab')2 anti-... | 10.1038/ncomms10682 | article | en | 2,016 | false | true | false | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | The two-dimensional trajectories of single molecules in the plane of focus were constructed by correlation analysis between consecutive images using a Vogel algorithm. QD-based trajectories were considered synaptic if co-localized with Homer 1C dendritic clusters for at least five frames. Fluorescence lifetime imaging ... | 10.1038/ncomms10682 | article | en | 2,016 | false | true | false | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | 4 objective using an appropriate green fluorescent (GFP) filter set. Cells were excited using a sinusoidally modulated 1-W 477-nm LED (light-emitting diode) at 40 MHz under wild-field illumination. Emission was collected using an intensified CCD LI2CAM camera (FAICM, Lambert Instruments). The phase and modulation were ... | 10.1038/ncomms10682 | article | en | 2,016 | false | true | false | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | Moderate stimulation intensity was assessed during an I/O protocol at the beginning of each recording. Synaptic changes were recorded in response to 10 pulse presynaptic stimulation at frequencies between 2 and 50 Hz at a holding potential of -70 mV. Slices were superfused with standard 32-°C aCSF, which was supplement... | 10.1038/ncomms10682 | article | en | 2,016 | true | false | false | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | Statistical significance (Prism 4. 0 (GraphPad)) between more than two normally distributed data sets was tested by one-way analysis of variance test followed by a Newman-Keuls test to compare individual pairs of data. Non-Gaussian data sets were tested by non-parametric Mann-Whitney test. Electrophysiology. Data were ... | 10.1038/ncomms10682 | article | en | 2,016 | true | true | false | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | A Student's t-test was applied when data passed the Kolmogorov-Smirnov test for normality. If not, significance was determined using a Mann-Whitney U-test. Significance of short-term-plasticity data was assessed using a two-way analysis of variance with Bonferroni post hoc testing. | 10.1038/ncomms10682 | article | en | 2,016 | true | false | false | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | Acknowledgements We thank Joost Hoetjes and Robert Zalm for genotyping; Frank Koopmans for proteomics support; the Bordeaux Imaging Center , part of the FranceBioImaging national infrastructure , for support in microscopy; the IINS cell biology core facility for providing neuronal cultures; and the Pole in vivo... | 10.1038/ncomms10682 | article | en | 2,016 | false | false | true | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | S. S. was supported by an NWO VICI grant ( ALW-Vici 016. 150. 673 ). H. D. M. was supported by an NWO VICI grant ( ALW-Vici 865. 13. 002 ) and the ERC grant BrainSignals ( 281443 ). D. C. , A. S. H. , F. C. , J. P. and C. R. were supported by funding from the Ministere de l'Enseignement Superieur et de la Re... | 10.1038/ncomms10682 | article | en | 2,016 | false | false | true | false | Neuroscience | https://openalex.org/fields/28 |
cc-by | Fungal DNA was extracted using a hydraulic press (Carver, Inc. , Wabash, USA) to lyse the cell walls and the Genomic-Tip 100/G kit (Qiagen 1 , CA, USA) following the manufacturer's protocol. DNA purity was assessed by measuring 260/280 and 260/230 absorbance ratios with a NanoDrop 1 spectrophotometer (2000; Thermo Scie... | 10.1371/journal.pone.0165656 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | PCR Multiplex Assembling For the assignment of PCR multiplexes we defined four amplification size ranges: 1) 100 to 115 bp; 2) 125 to 150 bp; 3) 155 to 170 bp; and 4) 180 to 210 bp. These groups were defined to separate the microsatellite markers by size and thus avoid interference between markers within each multiplex... | 10.1371/journal.pone.0165656 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | PCR reactions were performed separately for each multiplex in 12,5 μL volume containing 3 μL of DNA, ¼ of the recommended volume of multiplex PCR Kit (Qiagen 1 , USA), and 1. 25 μL of the primer mix. The primer mix included 2 μM of unlabelled forward and reverse primer and 0. 5 μM or 0. 75 μM of labelled forward primer... | 10.1371/journal.pone.0165656 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgments Concerning the M. saponariae samples, we would like to thank all collectors that helped us building such a large collection. We thank the GeT-Place INRA platform for sequencing and the GENTYANE INRA platform for assistance with fungal genotyping. We thank Antoine Branca for help in statistical ana... | 10.1371/journal.pone.0165656 | article | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Funded by MULTIVIR ANR-13-BSV7-0008 , Agence Nationale de Recherche, (JAS) and GenomeFun 309403, European Reserach Council, (TG). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | 10.1371/journal.pone.0165656 | article | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | All relevant data are within the paper and its Supporting Information files, except the DNA sequence used in this study is available in GenBank (accession number PRJEB11435). | 10.1371/journal.pone.0165656 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Bacterial community analysis Microbial diversity was investigated on a Sterivex TM filter previously stored at À20°C. The filter was crushed in liquid nitrogen and the DNA extracted with the Powersoil DNA Isolation kit (MoBio Laboratories, Inc. , Carlsbad, CA, USA) following the manufacturers' specifications. Genomic D... | 10.1111/1751-7915.12426 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Sequences displaying ambiguous bases were also eliminated. Operational taxonomic units (OTUs) were defined by clustering sequences displaying 97% similarity. Thus, singleton sequences and chimeras were eliminated. The final OTUs were subsequently classified taxonomically using BLASTn against a curated database derived ... | 10.1111/1751-7915.12426 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Capture probe design and synthesis A set of five 31-to 38-mers degenerate probes covering the bssA gene was designed from nine bssA nucleic sequences from strains belonging to Deltaproteobacteria, Firmicutes (EF123665, EF123667, EU780921, FO203503, EF123663, EF123662) and environmental sequences obtained with 7772F/854... | 10.1111/1751-7915.12426 | article | en | 2,016 | false | true | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Illumina MiSeq sequencing and data analysis Captured DNA products were sequenced using a single MiSeq 2 9 300 bp run (Illumina) according to the manufacturer's specifications (Genoscreen). All raw reads were scanned for library adaptors and quality filtered using PRINSEQ-lite PERL script (Schmieder and Edwards, 2011) ... | 10.1111/1751-7915.12426 | article | en | 2,016 | true | true | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | Nucleotide sequence accession numbers Sixty-five sequences (16S rRNA and bssA genes) have been submitted to GenBank under accession numbers KX576572 to KX576636. | 10.1111/1751-7915.12426 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | The SHS probes (Table S1 ) were designed from bssA sequences of sulphate-reducing bacteria belonging to the Deltaproteobacteria and the Firmicutes, as well as the three sequences previously amplified with the primers of Winderl et al. (2007) in enrichments with mono-aromatic hydrocarbons (KX576575, KX576576, KX57657... | 10.1111/1751-7915.12426 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | AssA NmsA BssA Fig. 3. Phylogenetic tree based on partial bssA-like amino acid sequences from deep aquifers used for geological natural gas storage (in bold; this study) compared with sequences from pure strains, enrichment cultures or environments retrieved in the databases. Sequences in bold were obtained by PCR from... | 10.1111/1751-7915.12426 | article | en | 2,016 | true | false | false | false | Environmental Science | https://openalex.org/fields/23 |
cc-by | 1999) even though linkage between sites may affect the distribution of mutations (Williamson and Orive 2002). Mutations of mild deleterious effect are abundant in some bacteria (Hughes 2005; Balbi et al. 2009). If bacterial evolution is dominated by these mutations then selection might not strongly affect demographi... | 10.1093/molbev/msw048 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | This bias may also result from sampling different environments (or patients) while analyzing a single isolate per site (thus disregarding differences in population sizes in each site). Finally, a very common type of mixed sampling bias is found in studies extensively sampling a sub-population and a small number of very... | 10.1093/molbev/msw048 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | ClonalFrame Analysis and Subsequent Skyline Plot ClonalFrame was used with default parameters on the results of ten simulations with recombination, no selection and no sampling bias. All ClonalFrame outputs were imported in the ClonalFrame GUI (Didelot and Falush 2007). The convergence of MCMC traces was visually asse... | 10.1093/molbev/msw048 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgments We thank Sylvain Brisse , Adam Eyre-Walker , and Guillaume Laval for comments on an earlier version of this manuscript. This project was financed by the Centre National de la Recherche Scientifique (CNRS) and the Institut Pasteur. G. A. and M. L. acknowledge support from the grant ANR-12-BSV7-00... | 10.1093/molbev/msw048 | article | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | The importance of review articles in making the voice of rare diseases heard: OJRD's 10th anniversary Ségolène Aymé "Join us in making the voice of rare diseases heard" is the slogan of Rare Disease Day 2016, a special day established by EURORDIS (http://www. rarediseaseday. org/). It takes place on the last day of Feb... | 10.1186/s13023-016-0456-5 | editorial | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Orphanet Journal of Rare Diseases (OJRD) is committed during this commemorative year and beyond, to publishing review articles on specific rare diseases with the goal of expanding knowledge among health professionals and caregivers. Such articles are highly valued by patients and caregivers, especially for very rare di... | 10.1186/s13023-016-0456-5 | editorial | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Most clinical guidelines can only be defined due to the gathering of expert opinions which is considered the lowest possible level of evidence, but is still much better that nothing. The Orphanet database provides access to 100 clinical guidelines covering 470 rare diseases, a very low number indeed. They were produced... | 10.1186/s13023-016-0456-5 | editorial | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | OJRD is inviting funders to support the development of clinical guidelines and will support their publication. For some rare diseases it is also indispensable to provide emergency guidelines for pre-hospital emergency care as well as for hospital emergency care, as some interventions can be decisive, negatively or posi... | 10.1186/s13023-016-0456-5 | editorial | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Patients suffering from a rare disease are more likely to need inpatient care, surgery and anaesthesia. Perioperative complications may occur in this setting, especially if the medical team lacks specific knowledge of the disease. Reliable information on orphan diseases is essential to avoid preventable diseaserelated ... | 10.1186/s13023-016-0456-5 | editorial | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgement Data from the Orphanet database were retrieved by Mrs Annie Olry who is thanked for her contribution. | 10.1186/s13023-016-0456-5 | editorial | en | 2,016 | true | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgments This work was funded by the NASA MMS project. The authors recognize the tremendous effort in developing and operating the MMS spacecraft and instruments and sincerely thank all involved. MMS data are open to the public. The IRAP contribution to MMS was supported by CNES. | 10.1002/2016gl068992 | article | en | 2,016 | false | false | true | false | Physics and Astronomy | https://openalex.org/fields/31 |
cc-by | Animals and phenotypes Data were collected from the French progeny-testing programs for artificial insemination (AI) using bulls from the Blonde d' Aquitaine and Limousin breeds. Three traits that are linked to maternal care were recorded on 2284 purebred females that were born between 2002 and 2011 and were the daught... | 10.1186/s12711-016-0223-z | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Genotypes In total, 2269 females (1259 Limousin and 1010 Blonde d' Aquitaine cows) were genotyped. Most of the females were genotyped using the customized low-density chip EuroG10K BeadChip ®. Only 249 Blonde d' Aquitaine cows were genotyped with the Bovine SNP50 BeadChip ® [medium-density (MD) chip]. Most of the sires... | 10.1186/s12711-016-0223-z | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | We used GS3 software [18] to perform these analyses. A total of 100,000 iterations were performed, with a burn-in of 20,000 iterations. The retained π value was equal to 0. 025 %, corresponding to 177 SNPs selected at each iteration among all the SNPs on the HD chip, in order to have a smaller number of SNP effects t... | 10.1186/s12711-016-0223-z | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | However, our results on the within-breed detection of QTL strongly questioned this initial assumption. First, we found no common QTL for MB across the two breeds. Second, our results provided strong evidence (logBF > 8) for only one QTL for MY, which was shared by the two breeds. This QTL was located on chromosome 3 (T... | 10.1186/s12711-016-0223-z | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Genotyping data for the Limousin breed did not allow us to clarify the very high genetic correlation between MY and US that was estimated based on performance and pedigree information. As a consequence, we assume that numerous pleiotropic regions with minor effects must explain the polygenic correlation, without any st... | 10.1186/s12711-016-0223-z | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgements The authors would like to thank all the MIDATEST and Idele technicians for performance records collected at the test stations in Casteljaloux and Moussours. Financial support for this work was provided by the MATERGEN project funded by the Conseil Régional Midi-Pyrénées Aquitaine. | 10.1186/s12711-016-0223-z | article | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | github. io/picard/) and GATK (v3. 5) (McKenna et al. , 2010) for indexing, sorting, removing PCR duplicates, and local-realignment. The per-based mapping depth was calculated by bedtools (v2. 25. 0) (Quinlan and Hall, 2010) and the sliding window plots (window size = 100 bp, step size = 50 bp) were created by in-hou... | 10.7554/elife.17903 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgements We thank Jun-ichi Nakayama for providing chromo domain expression plasmids, Matthieu Schapira for G9a plasmid, National BioResource Project (Japan) for providing the genomic DNA library, Charlie Hoffman for the cDNA library, and members of Jia lab for helpful discussions and critical reading ... | 10.7554/elife.17903 | article | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Gas chromatography (GC) Essential oil sample analysis was performed on Perkin-Elmer Clarus 500 gas chromatograph (FID) equipped two fused silica capillary columns (50 m x 0. 22 mm, 0. 25 μm film thickness) BP-1 (polydimethylsiloxane) and BP-20 (polyethylene glycol). The oven temperature was programmed as follow: 60-220... | 10.1080/14786419.2016.1219858 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | C Nuclear magnetic resonance (NMR) 13 C NMR analyses (essential oil and CC fractions) were performed on a Bruker AVANCE 400 Fourier Transform spectrometer operating at 100. 623 MHz for 13 C, equipped with a 5 mm probe, in deuterated chloroform (CDCl 3 ), with all shifts referred to internal tetramethylsilane (TMS). 13 ... | 10.1080/14786419.2016.1219858 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | For Multicolor Competition Assay, control cells and fluorescent shRNA cells were counted, mixed with a 1:1 ratio, and were left untreated or treated either with CDT wt or CDT H153A. After 6 days of culture, cells were collected and the fluorescent rate was analyzed by flow cytometry with a Miltenyi MACSQuant Analyser 1... | 10.1038/srep36022 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | H2AX antibody was purchased from Epitomics (3522-1), γ H2AX antibody from Merck/Millipore (05-636), ATR (SAB4200348), Lamin A/C (SAB4200236), β -actin (sc-47778) antibodies from Santa Cruz, XRCC1 (X0629), XRCC4 (HPA006801), XPA (SAB1406599-50UG) antibodies from SIGMA, FANCD2 (NB100-182) antibody from Novus Biologicals,... | 10.1038/srep36022 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Acknowledgements We are grateful to Dr Patrick Calsou for AA8, EM9 and MEF cells, Dr. Jean-Yves Masson for PD20 and PD20 D2 cells. We thank Pfizer for the gift of calicheamicin-γ 1, Pr Eric Oswald , Dr Jean-Philippe Nougayrède and Dr Frédéric Taieb for their help with the E. coli CDT-I purification. We a... | 10.1038/srep36022 | article | en | 2,016 | false | false | true | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | For experiments, slices were perfused (2-3 ml/min) at 31°C with oxygenated ACSF. Neurons were patched at 40x magnification using an upright microscope (Zeiss Axioscop FS2+). Patch-clamp recording pipettes (4-5 MΩ) were filled with intracellular solution (in mM): 140 K-gluconate, 10 HEPES, 4 ATP, 2 MgCl2, 0. 4 GTP and 0... | 10.1371/journal.pone.0151233 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Determination of the E-I balance Data were analysed off-line with Acquis1TM and ElphyTM (Biologic UNIC-CNRS, Gif-sur-Yvette, France). The E-I balance determination is based on the continuous measurement of conductance dynamics during the full-time course of the stimulus-evoked synaptic response. Briefly, we performed p... | 10.1371/journal.pone.0151233 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Statistical analysis Data reported are mean ± standard error of the mean (SEM) of n cells. Each individual cell was recorded in a single acute slice (3-4 slices/ animal per day) in order to avoid repeated exposure to the TBS protocol and/or drug application. When a LTP protocol was applied (TBS), post-TBS conductance i... | 10.1371/journal.pone.0151233 | article | en | 2,016 | true | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Immunohistochemistry Rats were deeply anesthetized with pentobarbital (6%) and perfused transcardially with phosphate buffer (PB) 0. 1 M (pH 7. 3) and paraformaldehyde (4%) supplemented with 0. 25% glutaraldehyde. The brain was post-fixed overnight in the same solution and finally cryoprotected with 30% sucrose in 0. 1... | 10.1371/journal.pone.0151233 | article | en | 2,016 | false | true | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
cc-by | Pharmacological inhibition of SR not only prevented the TBS-induced eLTP and iLTP as observed with RgDAAO (Fig 3B & 3E ) but also induced depression of the synaptic responses, as observed with 7Cl-KYN (Fig 3B & 3E ; IntgT: 75. 9 ± 7. 4% of baseline; IntgE: 77. 3 ± 7. 6% of baseline; IntgI: 75. 3 ± 8. 6% of baseline... | 10.1371/journal.pone.0151233 | article | en | 2,016 | true | false | false | false | Biochemistry, Genetics and Molecular Biology | https://openalex.org/fields/13 |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.