einarolafsson/spacr-eaf1-source-data

spaCR EAF1 manuscript source data

Source data accompanying Pooled image-based CRISPR screening identifies EAF1 as a regulator of host ESCRT recruitment by Toxoplasma gondii.

Authors: Einar B. Olafsson, Christophe-Sebastien Arnold, Jacob A. Kellermeier, Patrick A. Rimple, Hargobinder Kaur, Yifan Wang, Jonathan Z. Sexton, Staffan Svärd, Vern B. Carruthers, and Matthew J. O'Meara.

The study develops spaCR, a pooled image-based CRISPR screening workflow, and examines EAF1-dependent recruitment of host TSG101 during Toxoplasma gondii infection. These files support inspection and reanalysis of the figure-level measurements, screening outputs, classifier performance and attribution, genetic validations, and proteomic analyses.

Contents

This release contains all 331 source files (5.38 GB) supplied for the manuscript, preserving their original filenames, directory structure, and bytes. The repository also provides this dataset card, a file index, and checksums.

The source_data_index.csv lists each source file, its size in bytes, its SHA-256 checksum, and its top-level figure group. The dataset viewer displays this file index; its train split is an indexing convention and does not designate training data. Download individual files from the Files and versions tab or retrieve the entire repository using the commands below.

Directory	Files	Size (MB)
Figure_1_and_Figure_S1/	1	0.54
Figure_3/	21	123.16
Figure_4/	139	2417.12
Figure_5/	43	179.92
Figure_5B_C_XGBoost_single_cell_scores/	4	264.27
Figure_5B_C_and_Figure_S5_MaxViT_single_cell_scores/	4	19.16
Figure_5C_and_Figure_S7_MaxViT_parasite_matched_rerun/	2	0.37
Figure_5J_K_and_Figure_S10A_E_IPMS/	19	0.58
Figure_5_classifier_cell_populations/	1	0.00
Figure_S1/	7	35.04
Figure_S3/	9	48.42
Figure_S5/	9	54.57
Figure_S6/	14	5.39
Figure_S7/	16	3.27
Figure_S8/	3	32.30
Figure_S9/	2	0.07
Shared_Archival_Inputs/	6	1835.11
Shared_Model_Attribution/	24	5.84
Shared_Validation/	1	3.80
Table_S6_colocalization_versus_parasite_burden/	1	3.99
source_data_uploade/	5	351.87

Figure and panel names identify the associated manuscript panels. CSV/TSV/XLSX files contain measurements, replicate summaries, statistics, and analysis outputs. TIFF/PNG/JPEG/ND2/PDF files provide image or figure source material. ZIP files contain archival measurement databases, model checkpoints, saliency images, or packaged submission files. The source_data_uploade/ directory retains the original submission ZIP packages; these can overlap with files elsewhere in the repository and should not be treated as additional independent observations.

Experimental units and metadata

The pooled library originated from one transfection and was partitioned across four plates. NC and PC control infections used separate biological replicates for each plate; all four screening plates were infected concurrently. Genetic-validation tables report independent biological-replicate summaries: n = 3 per condition for Figure 5D and Figure S6C–G, and n = 4 for Figure 5E–F. EAF1 IP-MS used four independent infections per strain, harvested and immunoprecipitated separately.

In Figure_3/Panel_B/Figure_3B_biological_replicate_means.csv and Figure_3/Panel_B/Figure_3B_well_level_values.csv, the column named experiment_date records analysis dates, as confirmed by the authors. These are not the dates of infection. The original column names are retained in this release.

Download

Download the version cited with this source-data release:

hf download einarolafsson/spacr-eaf1-source-data --repo-type dataset --revision v1.0 --local-dir spacr-eaf1-source-data

Or use Python:

from huggingface_hub import snapshot_download

snapshot_download(
    repo_id="einarolafsson/spacr-eaf1-source-data",
    repo_type="dataset",
    revision="v1.0",
    local_dir="spacr-eaf1-source-data",
)

After downloading, verify the source files from the repository directory:

sha256sum --check SHA256SUMS

ZIP archives can be extracted with standard archive tools. CSV/TSV files can be read with pandas or other tabular-analysis tools; XLSX files can be opened in spreadsheet software. Use compatible microscopy software for ND2 files and image-analysis tools for TIFF/PNG images. Files are provided in their original analysis formats rather than as one combined training dataset.

Related data and software

The manuscript reports the following complementary data deposits:

• Sequencing: NCBI BioProject PRJNA1261935, SRA SRR33531217–SRR33531220.
• High-content images: BioStudies S-BIAD2135.
• Proteomics: ProteomeXchange/PRIDE PXD080696.
• spaCR: GitHub v1.5.1.6 and Zenodo software archive.
• spaCRPower: GitHub, commit d358b6dd2323fafa807be002d556845a6fb3c7f2.

Citation and version

Olafsson, E. B., et al. (2026). Source data for “Pooled image-based CRISPR screening identifies EAF1 as a regulator of host ESCRT recruitment by Toxoplasma gondii”. Version 1.0. Hugging Face. https://huggingface.co/datasets/einarolafsson/spacr-eaf1-source-data/tree/v1.0

The v1.0 tag identifies this release. A paper DOI can be added when available.

License

No reuse license has been specified for this release.