| # Download |
|
|
| Download the stimulus data from the [eyewire2-data Hugging Face dataset](https://huggingface.co/datasets/eulerlab/eyewire2-data/tree/main/stimuli). |
|
|
| # Stimulus description |
| In Eyewire2 stimuli were chirp, moving bar (MB) and mouse camera movies (MCs). |
|
|
| The original QDSpy stimulator scripts for [chirp](./QDSpy/Chirp.py), [MB](./QDSpy//DS.py) |
| and [MCs](./QDSpy/MouseCam_Right.py) are included in this folder. |
|
|
| ## Chirp |
| The chirp had a spot with a diameter of 1000 µm and otherwise follows previous publications. |
| [Chirp PDF](./global_chirp/chirp1000_setup3.pdf) |
|
|
| ## Moving bar |
| The moving bar directions in the script were (0, 180, 45, 225, 90, 270, 135, 315) degrees. |
| In the setup chamber this resulted in the following directions of the moving bar (↓, ↑, ↙, ↗, ←, →, ↖, ↘), where e.g. "↓" means back to front. |
| The bar width (the dimension that is orthogonal to the direction of motion) was 300 µm, the speed 1000 µm/s, and the length 1000 µm. |
| [Moving Bar PDF](./moving_bar/DS_setup3.pdf) |
|
|
| ### Retina orientation |
| The retina was from the right eye. Ventral was in the back of the setup chamber. |
| So this is the orientation of the retina in the setup chamber: |
| ``` |
| ----- Ventral ---- |
| Nasal ---- Temporal |
| ----- Dorsal ----- |
| ``` |
| For the MB, 0° therefore means ventral to dorsal motion. |
| 90° means temporal to nasal motion. |
|
|
| In the manuscript the retina is depicted as (i.e. with a 180° rotation compared to the setup chamber): |
| ``` |
| ------ Dorsal ----- |
| Temporal ---- Nasal |
| ------ Ventral ---- |
| ``` |
| In this projection (0, 180, 45, 225, 90, 270, 135, 315) corresponds to (↑, ↓, ↗, ↙, →, ←, ↘, ↖). |
|
|
| ## Mouse camera movies |
| MCs were similar as in [Höfling et al. 2024](https://elifesciences.org/articles/86860) which were derived from recordings by [Qiu et al. 2021](https://www.cell.com/current-biology/fulltext/S0960-9822(21)00676-X). |
| There are different MC stimuli, e.g. MC-16 and MC-20, that are all based on the same 113 five-second movie clips. |
| Each MC stimulus MC-X is described by a sequence [test/train1-X/test/train2-X/test](https://iiif.elifesciences.org/lax:86860%2Felife-86860-fig1-v1.tif/full/1500,/0/default.jpg), |
| where: |
| - the test sequence, consisting of five movie clips, is the same for all MC versions and is shown three times (the beginning, the middle and the end), |
| - and the train sequences train1-X and train2-X consist of 54 movie clips, each, that are differently ordered for each MC stimuli MC-X. |
|
|
| Each MC therefore consists of a sequence of 123 five-second movie clips, with 123 corresponding triggertimes. |
| The order of the train sequences is stored in [RandomSequences.txt](). |
| With [mc_to_numpy.py]() you can generate numpy arrays that correspond to the MC stimuli. |
| With [mc_arrays/load_mc_array.ipynb]() you can display them as they would appear in QDSpy. |
| In the setup chamber the movies were rotated by 90 degrees counter-clockwise. |
|
|
