| # Stimulus description
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| In Eyewire2 stimuli were chirp, moving bar (MB) and mouse camera movies (MCs).
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| The original QDSpy stimulator scripts for [chirp](./QDSpy/Chirp.py), [MB](./QDSpy//DS.py)
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| and [MCs](./QDSpy/MouseCam_Right.py) are included in this folder.
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|
| ## Chirp
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| The chirp had a spot with a diameter of 1000 µm and otherwise follows previous publications.
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| [Chirp PDF](./global_chirp/chirp1000_setup3.pdf)
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|
|
| ## Moving bar
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| The moving bar directions in the script were (0, 180, 45, 225, 90, 270, 135, 315) degrees.
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| In the setup chamber this resulted in the following directions of the moving bar (↓, ↑, ↙, ↗, ←, →, ↖, ↘), where e.g. "↓" means back to front.
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| The bar width (the dimension that is orthogonal to the direction of motion) was 300 µm, the speed 1000 µm/s, and the length 1000 µm.
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| [Moving Bar PDF](./moving_bar/DS_setup3.pdf)
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|
|
| ### Retina orientation
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| The retina was from the right eye. Ventral was in the back of the setup chamber.
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| So this is the orientation of the retina in the setup chamber:
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| ```
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| ----- Ventral ----
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| Nasal ---- Temporal
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| ----- Dorsal -----
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| ```
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| For the MB, 0° therefore means ventral to dorsal motion.
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| 90° means temporal to nasal motion.
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| In the manuscript the retina is depicted as (i.e. with a 180° rotation compared to the setup chamber):
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| ```
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| ------ Dorsal -----
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| Temporal ---- Nasal
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| ------ Ventral ----
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| ```
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| In this projection (0, 180, 45, 225, 90, 270, 135, 315) corresponds to (↑, ↓, ↗, ↙, →, ←, ↘, ↖).
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|
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| ## Mouse camera movies
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| MCs were similar as in [Höfling et al. 2024](https://elifesciences.org/articles/86860) which were derived from recordings by [Qiu et al. 2021](https://www.cell.com/current-biology/fulltext/S0960-9822(21)00676-X).
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| There are different MC stimuli, e.g. MC-16 and MC-20, that are all based on the same 113 five-second movie clips.
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| Each MC stimulus MC-X is described by a sequence [test/train1-X/test/train2-X/test](https://iiif.elifesciences.org/lax:86860%2Felife-86860-fig1-v1.tif/full/1500,/0/default.jpg),
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| where:
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| - the test sequence, consisting of five movie clips, is the same for all MC versions and is shown three times (the beginning, the middle and the end),
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| - and the train sequences train1-X and train2-X consist of 54 movie clips, each, that are differently ordered for each MC stimuli MC-X.
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| Each MC therefore consists of a sequence of 123 five-second movie clips, with 123 corresponding triggertimes.
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| The order of the train sequences is stored in [RandomSequences.txt]().
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| With [mc_to_numpy.py]() you can generate numpy arrays that correspond to the MC stimuli.
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| With [mc_arrays/load_mc_array.ipynb]() you can display them as they would appear in QDSpy.
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| In the setup chamber the movies were rotated by 90 degrees counter-clockwise.
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