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1,"Literature-based approach with an integrated human PFC atlas; selected cell-type markers from literature and added markers for neuronal activity and neuropathologies (Alzheimer's, Schizophrenia)."
2,"Reference dataset only; applied the Persist algorithm to select top 50/100/150 genes, algorithmic without manual curation."
3,Submitted a previously designed panel (incorrect tissue).
4,"Combined reference dataset with literature; gene enrichment ranked with Cohen's mean, supplemented with known marker genes for key cell types."
5,"Reference dataset only; iterative greedy algorithm for kNN-graph reconstruction, focused on a graph-based representation of the data."
6,Alternative dataset (not the provided one); selected genes to differentiate predicted cell types by integrating scRNA-seq with Visium data and spot-level deconvolution.
7,Multiple datasets (provided + cell-type-specific studies); cell-type markers from multiple references plus DE genes; included genes targeting intracellular pathways and cross-referenced markers across studies.
8,Reference dataset only; selected highly variable genes with optimal normalization techniques.
9,"Literature only; cell-type markers from broad and targeted studies, including presynaptic markers, transcription factors, and Schizophrenia risk genes in GRNs."
10,"Reference dataset only; selected top DE genes per cell type, focused on differential expression."
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