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方法：回顾性分析2009年1月至2018年1月在中南大学湘雅医院、湘雅二医院及湘雅三医院感染病科收治的183例DILF患者的临床资料，根据其预后分为好转组(n=67)及无效组(n=116)。 | Methods: A retrospective analysis of clinical data was performed in 183 hospitalized patients, who were diagnosed with DILF in Xiangya Hospital, the Second Xiangya Hospital and the Third Xiangya Hospital, Central South University from January 2009 to January 2018. The patients were divided into an improved group (n=67)... |
使用单因素分析对年龄，Tbil，SCr，PT及并发症等可能的预后影响因素进行初筛，基于单因素分析结果，进一步使用多因素分析筛选出独立的预后因素并构建LRM模型。 | Univariate analysis was performed to screen for possible prognostic factors such as age, Tbil, SCr, PT and complications. According to the results of univariate analysis, the multivariate analysis was performed to determine the independent prognostic factors and construct a LRM. |
将LRM模型与终末期肝病(model for end-stage liver disease，MELD)模型进行比较，采用受试者工作曲线(receiver operating characteristic curve，ROC)评估LRM模型和MELD模型的预测价值，比较两模型间ROC曲线下面积(area under the ROC curve，AUC)及总正确率等参数的优劣，并使用独立样本进行验证。 | The LRM was compared with the model for end-stage liver disease (MELD), the predictive value of LRM and MELD was evaluated by receiver operating characteristic curve (ROC), the parameters such as area under the ROC (AUC) and total accuracy were compared between the 2 models and verified by another independent sample. |
结果：单因素分析提示：年龄，临床分型，肝性脑病，肝肾综合征，WBC计数，天门冬氨酸转氨酶/谷丙氨酸转氨酶(aspartic acid transaminase/glutarine transaminase，AST/ALT)比值，Tbil，SCr，PT和甲胎蛋白(alpha-fetoprotein，AFP)等指标在两组间的差异均有统计学意义(均P<0.05)。 | Results: According to univariate analysis, there was significant differences in age, clinical type, hepatic encephalopathy, hepatorenal syndrome, WBC count, the ratio of aspartic acid transaminase (AST) to glutamine transaminase (ALT) (AST/ALT), Tbil, SCr, PT and alpha-fetoprotein (AFP) between the 2 groups (all P<0.05... |
多因素分析提示：AFP，PT，AST/ALT比值，肝性脑病和肝肾综合征为DILF患者独立的预后影响因素，可用于构建LRM模型。 | Multivariate analysis revealed that: AFP, PT, AST/ALT, hepatic encephalopathy and hepatorenal syndrome were independent prognostic factors for DILF, which could be applied to constructing a LRM. |
LRM模型和MELD模型的AUC分别为0.917(95% CI：0.876~0.959)和0.709(95% CI：0.633~0.786)，总正确率分别为86.7%和68.3%，差异有统计学意义(P<0.05)。 | The AUC of LRM and MELD was 0.917 (95% CI 0.876 to 0.959) and 0.709 (95% CI 0.633 to 0.786) respectively, the total accuracy rate of prediction for the LRM and the MELD was 86.7% and 68.3% respectively, there was significant difference in AUC and total accuracy rate between the LRM and the MELD (P<0.05). |
结论：AFP，PT，AST/ALT比值，肝性脑病和肝肾综合征为DILF患者独立的预后影响因素；LRM模型能较准确地预测DILF患者的短期预后，其应用价值优于MELD模型。 | Conclusion: AFP, PT, AST/ALT, hepatic encephalopathy and hepatorenal syndrome were independent prognostic factors for DILF; the LRM can well predict the prognosis in the DILF patients, which is superior to the MELD. |
本文論述長照個案口腔健康照護的三大理念：口腔狀況與全身健康的關聯性、口腔照護與吞嚥困難、口腔健康與水化程度，從而提出長照機構口腔健康照護策略，期使引發長照夥伴們對口腔健康的重視，及落實長照個案的口腔健康照護。 | Oral health impacts the physical and psychological comfort and the quality of life of clients in long-term care. |
The programs to prevent or delay disability care advocated under Taiwan's Long-Term Care Plan 2.0 address oral health care, highlighting the importance of oral health care for clients in long-term care. | |
This article discusses three concepts of oral health care for clients who are in long-term care. These include: the relationship between oral conditions and general health, oral care and dysphagia, and oral health and hydration. In addition, oral health care strategies for long-term care facilities are discussed. It is... | |
遗传代谢性肝病临床表型差异显著，尽管每种疾病的发病率不高，由于疾病谱广，总体受累人群并不少见，临床应予重视。 | The clinical phenotype of genetic liver disease is significantly different. Although the incidence of disease is low, because of the wide spectrum of diseases, the overall affected population is not rare. Therefore, clinical concern is a matter of alarm. |
这类疾病临床罕见，相对认识不足，临床误诊问题较为突出，现以临床实践过程中所遇到的问题为出发点，探讨一下遗传代谢性肝病的临床基因诊断。 | Additionally, these diseases rarity in clinical practice has covered the recognition, causing increased problem of clinical misdiagnosis. In accordance with the problems encountered in clinical practice of our hospital, we explore the clinical diagnosis of genetic liver disease. |
目前全球已有超过30万人植入人工耳蜗，随着植入适应证的放宽，植入时、植入后如何良好保留患者的残余听力和内耳原有微结构及功能，如何安全有效地借助药物减轻术后炎性反应和纤维化、保留残余听力、减少毛细胞和螺旋神经节细胞的凋亡等已成为该领域的研究热点。 | Nowadays, more than 300 000 deaf people around the world benefit from cochlea implantation. With the extension of cochlear implantation indications, it has become a research focus for how to apply the anti-inflammatory and anti-fibrotic drugs safely and effectively to better retain residual hearing, as well as to prote... |
本文将内耳局部给药方法分为鼓室内直接给药、鼓室内缓释载体给药、耳蜗内给药和人工耳蜗植入电极的修饰四大类，并分别就其研究结果及前沿进展做一综述。 | In this review, the methods of topical drug delivery to the inner ear are divided into four categories: transtympanic injection of liquid solutions, tympanic implantation of drug delivery systems, intracochlear injection and modified implant electrode. The results in each category and up to date progress are reviewed. |
SIRT7 是哺乳动物Sirtuins 家族中的一员，定位于核仁，是一种高度特异性的H3K18Ac (组蛋白H3 的乙酰化18 位赖氨酸残基) 去乙酰化酶。 | As a member of the Sirtuins family in mammals, SIRT7 locates in nucleus and is a highly specific H3K18Ac (acetylated lysine 18 of histone H3) deacetylase. |
近年来的研究发现SIRT7 可通过多种途径参与调控核糖体RNA 转录、细胞代谢、细胞应激以及DNA 损伤修复等生理过程。 | Recent studies showed that SIRT7 could participate in the ribosomal RNA transcription, cell metabolism, cell stress and DNA damage repair through various signaling pathways. |
此外，SIRT7 还与衰老、心脏疾病及脂肪肝等密切相关。 | In addition, SIRT7 is also closely related with aging, heart disease and fatty liver. |
特别是SIRT7 在多种肿瘤如肝癌、胃癌、乳腺癌、膀胱癌、结直肠癌、胰腺癌和头颈鳞状细胞癌等发生发展中起着重要的调节作用。 | In particular, SIRT7 plays important roles in the regulation of initiation and development of various tumors, such as liver cancer, gastric cancer, breast cancer, bladder cancer, colorectal cancer, and head/neck squamous cell carcinoma. |
文中综述了SIRT7 的细胞及分子生物学作用，并系统总结了其在人类疾病中的研究现状。 | This review describes the cellular and molecular functions of SIRT7, and systematically summarizes recent progress of SIRT7 in human disease. |
猪圆环病毒2 型编码的ORF4 蛋白是近年来发现的新蛋白。 | A novel protein encoded by the open reading frame 4 (ORF4) was recently discovered in porcine circovirus type 2 (PCV2). |
本研究首先构建了带双标签的真核表达载体pCMV-N-Flag-GST，再将ORF4 基因插入该载体中，形成pCMV-N-Flag-GST-ORF4。 | In the present study, the ORF4 was inserted into the multiple cloning site of pCMV-N-Flag-GST, yielding recombinant plasmid pCMV-N-Flag-GST-ORF4. |
将质粒转染293T 细胞表达ORF4 后，通过GST Pull-down 试验捕获细胞内潜在与ORF4 互作的蛋白库。 | The recombinant plasmid was transfected into 293T cells and the intracellular interaction complex of ORF4 were enriched and separated by GST pull-down and SDS-PAGE, sequentially. |
经SDS-PAGE 分离及银染后，对所得的特异性条带进行质谱鉴定，筛选出5 个与ORF4 潜在互作的蛋白，包括丝氨酸/苏氨酸蛋白磷酸酶6 催化亚基、α 心肌蛋白、β 肌动蛋白、SEC-14 样蛋白5 和肌球蛋白myosin 9。 | The potential interacting proteins of PCV2 ORF4 were stained with silver and identified by mass spectrometry (MS). Finally, five candidate ORF4-interacting proteins, including Serine/threonine-protein phosphatase 6 catalytic subunit, alpha cardiac muscle 1, actin, SEC14-like protein 5 and myosin 9 were identified. |
上述研究结果为深入揭示ORF4 在病毒感染细胞过程中发挥的作用提供新的思路与方向。 | These results would benefit a better understanding of the biological function of ORF4 in PCV2 infected cells. |
聚3- 羟基丁酸乳酸酯[Poly(3-hydroxybutyrate-co-lactate) ， P(3HB-co-LA)] ， 属于聚羟基脂肪酸酯(Polyhydroxyalkanoates，PHA) 家族的一员，是一种具有良好生物相容性和可降解性的天然高分子生物材料。 | Poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] belongs to the polyhydroxyalkanoates (PHA) family and possesses promising properties including biocompatibility and biodegradability. |
文中通过在大肠杆菌中引入来源于富养罗尔斯通氏菌Ralstonia eutropha 的β-酮硫解酶、乙酰乙酰CoA 还原酶、来源于丙酸梭菌Clostridium propionicum 的丙酰CoA 转移酶突变体以及荧光假单胞菌Pseudomonas fluorescens strain 2P24 来源的PHA 合成酶突变体等异源酶，成功实现了一步法利用葡萄糖合成P(3HB-co-LA)，其中乳酸组分的摩尔百分比达到1.6%，聚合物含量为83.9 wt%。 | In this study, we directly synthesized P(3HB-co-LA) with glucose by introducing the β-ketothiolase and acetoacetyl-CoA reductase from Ralstonia eutropha, the engineered propionate CoA transferase from Clostridium propionicum and the engineered polyhydroxyalkanoate synthase from Pseudomonas fluorescens strain 2P24 into ... |
在此基础上，通过敲除辅酶Q8 合成所需的黄素异戊烯基转移酶基因 (ubiX) 来弱化呼吸链水平，从而增强乳酸积累，并进一步缺失乳酸脱氢酶基因 (dld) 以减少乳酸在发酵后期转化成丙酮酸，最终将P(3HB-co-LA) 中乳酸组分的摩尔百分比提高至14.1%，而聚合物含量为81.7 wt%。 | On this basis, in order to accumulate lactate, we reduced the activity of respiratory chain by deleting the ubiX gene, which is involved in the synthesis of coenzyme Q8. Moreover, we removed the dld gene to avoid the conversion of lactate to pyruvate during the fermentation. With these manipulations, the molar percenta... |
上述实验结果表明，采用弱化呼吸链水平策略可有效提高聚合物中乳酸组分的摩尔百分比，从而提供了一种改变生物合成聚合物中单体组分含量的新思路。 | The test results indicated that the strategy of reducing the activity of respiratory chain effectively increased the lactate units in the polymer, and it contributed a new approach to change the content of monomer components in the polymer. |
转运核糖核酸 (tRNA) 是蛋白质合成过程中重要参与成分之一，为了探索稀有密码子对应的tRNA (稀少tRNA) 丰度改变对外源基因表达量的影响，文中构建了毕赤酵母稀少tRNA 基因与外源基因共表达体系。 | Translocation ribonucleic acid (tRNA) is one of the important components in protein synthesis. In order to explore the effect of the changes of tRNAs corresponding to rare codons (rarity tRNAs) on the expression of exogenous genes, the co-expression system of rare tRNA gene and exogenous gene in Pichia pastoris was con... |
首先在GFP 基因中添加由4 个连续脯氨酸稀有密码子CCG 组成的阻遏区，结果显示该GFP 基因的表达量明显降低。 | The expression of GFP in P. pastoris can be greatly reduced when a repressor region composed of four continuous proline rare codon CCG was added into the GFP gene. |
然后将带有阻遏区的GFP 基因和tRNAProCCG基因顺次连接于pPIC9K 载体上，在毕赤酵母GS115 中共表达，结果使GFP表达量提高了4.9%；另将带有阻遏区的GFP 基因和tRNAProCCG基因分别连接于pPIC9K 和pFLDα 载体，在毕赤酵母GS115 中共表达，GFP 表达量最高提高了12.5%；应用同样方式将tRNAProCCG基因与NFATc3T-GFP 融合基因共表达，其表达量提高了21.3%。 | The expression amount of the repressed GFP could be increased about 4.9% when tRNAProCCG gene was cointegrated to the 3' of the repressed GFP gene through pPIC9K to the genome of P. pastoris GS115. Meanwhile, the expression amount of the repressed GFP increased about 12.5% by integrating the repressed GFP gene and tRNA... |
可见，tRNAProCCG在毕赤酵母GS115 中确为稀少tRNA，通过共表达tRNAProCCG基因可显著提高带有连续该密码子的外源基因表达量，并且，文中构建的共表达体系将同样适用于其他稀少tRNA 基因的筛选和验证。 | In conclusion, tRNAProCCG gene has been confirmed to be a kind of rare tRNAs in P. pastoris GS115. Through co-expression of tRNAProCCG gene and heterologous genes which containing the continuous rare codon CCG, the expression of the repressed heterologous genes could be increased significantly. Furthermore, this co-exp... |
防御素是一类富含精氨酸和半胱氨酸的内源性阳离子抗菌肽，是软体动物抵御各种病原微生物侵染的重要免疫因子。 | Defensins are endogenous cationic antimicrobial peptides rich in arginine and cysteine residues. They are important immune factors resisting pathogenic bacteria infection for mollusks. |
太平洋牡蛎防御素 (Crassostrea gigas defensin，CgD) 近羧基端的43 个氨基酸残基构成了其成熟肽区域，决定了CgD 的生物学活性。 | The 43 amino acid residues near the carboxyl terminal for Crassostrea gigas defensin (CgD) form its mature peptide region, responsible for the biological activity of CgD. |
抑菌试验结果显示分别含重组蛋白CgDH⁺和重组蛋白CgDH⁻的培养液上清对金黄色葡萄球菌Staphylococcus aureus 和铜绿假单孢菌Pseudomonas aeruginosa 都具有抑菌活性，表明重组蛋白中6×His 标签的存在与否并不影响其生物学活性。 | Antimicrobial assay showed that the culture medium supernatant containing recombinant CgDH⁺ and recombinant CgDH-, respectively, had activities against Staphylococcus aureus and Pseudomonas aeruginosa, indicating that the existence of 6×His tag in the recombinant proteins do not affect their biological activities. |
甲状腺肿瘤是目前最常见的疾病，而甲状腺手术日益增多。 | Thyroid tumors are one of the most common diseases, and the thyroidectomy surgery increases. |
现今日间手术已成为外科手术新的趋势，那么日间手术是否适宜甲状腺手术？本文通过对医院、医生、手术、患者等方面探讨，分析其安全性。 | The outpatient surgery is the new trend in clinic. Is it suited for the thyroidectomy? In this article, we will analyse the setting of hospital, surgeon, surgery, patients, and so on, to prove the safety for the outpatient thyroidectomy. |
在前期工作中发现，截短的轮状病毒VP4*蛋白 (aa 26–476) 在大肠杆菌中能够以可溶形式表达，且在小鼠模型中具有较高的免疫原性和免疫保护性。 | In previous studies, we found that truncated rotavirus VP4* (aa 26-476) could be expressed in soluble form in Escherichia coli and confer high protection against rotavirus in the mouse mode. |
本研究通过颗粒化进一步提高VP4*蛋白的免疫保护性。 | In this study, we further improved the immunogenicity of VP4* by polymerization. |
综上所述，VP4*高聚体的研究为轮状病毒基因工程亚单位疫苗的研制提供了更广阔的思路。 | In summary, the study in VP4* polymers provides a new strategy for the development of recombinant rotavirus vaccines. |
为探讨27nt-miRNA 对间充质干细胞向血管平滑肌细胞分化影响，构建27nt-miRNA 过表达、反义序列Anti-27nt-miRNA 以及阴性对照的表达质粒，慢病毒包装后分别转染人脐带间充质干细胞 (hUCMSC)，加入Ⅳ型胶原诱导hUCMSC 定向分化为血管平滑肌细胞。 | To investigate the effect of 27nt-miRNA on the differentiation of mesenchymal stem cells into vascular smooth muscle cells. The highly expression plasmids of 27nt-miRNA and anti-27nt-miRNA, and negative control plasmids were constructed, packaged with lentivirus and transfected into human umbilical cord mesenchymal ste... |
经检测，27nt-miRNA 过表达分化组与阴性对照组相比，细胞活力下降20.48% (P<0.05)，SMA mRNA、SM22α mRNA及其蛋白质表达量明显升高 (P<0.05)；而Anti-27nt-miRNA 分化组细胞活力上升了18.07% (P<0.05)，SMA mRNA、SM22α mRNA 及其蛋白质表达量下降 (P<0.05)。 | Compared with the negative control group, the viability of the 27nt-miRNA overexpression group was decreased by 20.48% (P<0.05), and the expression of SMA mRNA and SM22α mRNA and protein was significantly increased (P<0.05); the viability of Anti-27nt-miRNA group was increased 18.07% (P<0.05), and the expression of SMA... |
综上所述，27nt-miRNA 能够促进间充质干细胞向血管平滑肌细胞分化，并且抑制分化后的细胞活力。 | In summary, 27nt-miRNA promotes mesenchymal stem cells differentiation into vascular smooth muscle cells and inhibits cells viability. |
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