| > Research Use Only (RUO). Not for use in diagnostic procedures or patient care. | |
| # qPCR for gene expression (example template) | |
| **Sources**: DOI:xx.xxxx/xxxx; Preprint: <link> | |
| ## Equipment | |
| - qPCR thermocycler | |
| - Calibrated micropipettes (P10, P200) | |
| ## Reagents (example) | |
| - SYBR Green Master Mix — 1x final | |
| - Forward primer (10 µM); Reverse primer (10 µM) | |
| - cDNA template | |
| ## Steps | |
| 1. Thaw reagents on ice (≈10 min). Mix by gentle inversion. | |
| 2. Prepare master mix (per 20 µL reaction): 10 µL SYBR mix; 0.8 µL fwd primer; 0.8 µL rev primer; x µL template; nuclease-free water to 20 µL. | |
| 3. Plate reactions; seal; brief spin. | |
| 4. Thermocycler program: 95 °C 2 min; 40 cycles of [95 °C 15 s, 60 °C 60 s]; melt curve. | |
| ## Safety notes | |
| - Wear PPE; handle reagents per SDS. | |
| ## Bill of Materials (example) | |
| - SYBR mix (generic), Alt1: Vendor A cat#..., Alt2: Vendor B cat#... | |