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37320387
Wilson's & North Eastern Railway Shipping Co. Ltd
https://en.wikipedia.org/w/index.php?title=Wilson's%20&%20North%20Eastern%20Railway%20Shipping%20Co.%20Ltd
Wilson's & North Eastern Railway Shipping Co. Ltd Wilsons sought a solution to the Hull services problem and a joint venture was proposed. The negotiations were led by Charles Wilson (Lord Nunburnholme) and his brother Arthur Wilson who was also a Director of the N.E.R. - 1906 – Joint company formed in March to operat...
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Wilson's & North Eastern Railway Shipping Co. Ltd
https://en.wikipedia.org/w/index.php?title=Wilson's%20&%20North%20Eastern%20Railway%20Shipping%20Co.%20Ltd
Wilson's & North Eastern Railway Shipping Co. Ltd brother Arthur Wilson who was also a Director of the N.E.R. - 1906 – Joint company formed in March to operate services from Hull. In the same year Thomas Wilson Sons & Co. took control of local shipbuilders Earle's Shipbuilding. - 1923 – January – The company came und...
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Accommodation bridge
https://en.wikipedia.org/w/index.php?title=Accommodation%20bridge
Accommodation bridge Accommodation bridge An accommodation bridge or occupation bridge in the United Kingdom preserves a pre-existing private road, path or right of access when a major transport route is built across it. Without the bridge, access would be disrupted. Accommodation bridges are usually built at the cost...
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Accommodation bridge
https://en.wikipedia.org/w/index.php?title=Accommodation%20bridge
Accommodation bridge with short lengths of canal built to bypass obstacles, such as weirs and millponds. The river represented a long-established and accepted boundary, but these new sections were resented by landlords. Unlike turnpike roads, drovers could not simply cross the new road but now needed a bridge. These b...
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Accommodation bridge
https://en.wikipedia.org/w/index.php?title=Accommodation%20bridge
Accommodation bridge accommodation bridges, in the UK at least, were constructed during the railway building boom of the mid-19th century. British practice avoided level crossings wherever possible, except in the flat parts of the country where building a raised approach to a bridge would be more costly. Nevertheless t...
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Accommodation bridge
https://en.wikipedia.org/w/index.php?title=Accommodation%20bridge
Accommodation bridge Britain an accommodation bridge is the name for a bridge connecting land that belonged to one owner severed by the railway. An occupation bridge is the name for a bridge that carried an existing private road or took the railway over an existing private road. Similar terms were applied to level cros...
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Accommodation bridge
https://en.wikipedia.org/w/index.php?title=Accommodation%20bridge
Accommodation bridge e for a bridge connecting land that belonged to one owner severed by the railway. An occupation bridge is the name for a bridge that carried an existing private road or took the railway over an existing private road. Similar terms were applied to level crossings. # Motorways. Accommodation bridge...
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Oh Look Out
https://en.wikipedia.org/w/index.php?title=Oh%20Look%20Out
Oh Look Out Oh Look Out Oh Look Out is an American rock band based in Austin, TX created by singer/songwriter JP Pfertner in 2011. To date, Oh Look Out has released two full-length albums, and is described as "Catchy indie rock/pop with waves of fuzzy guitars and Casio keyboards that hit your brain like an Atari blast...
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Oh Look Out
https://en.wikipedia.org/w/index.php?title=Oh%20Look%20Out
Oh Look Out studio. The result was a mix of energetic indie rock/pop songs, drawing on inspiration from The Cars, The Rentals, and DEVO, as well as melancholy keyboard pop songs that draw comparisons to the bedroom recording legend, Daniel Johnston. The day after "Alright Alright Alright Alright Alright" was released,...
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Oh Look Out
https://en.wikipedia.org/w/index.php?title=Oh%20Look%20Out
Oh Look Out ongs that all connect together with waves of crunchy guitars, punchy keys, big drums and lyrics about losing your mind, exploding, reliving better years, and wearing out everything that you love. Songs spin backwards, Mellotrons play alongside moogs, cassette tape samples blast through jambox speakers, and ...
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Hulk: Gray
https://en.wikipedia.org/w/index.php?title=Hulk:%20Gray
Hulk: Gray Hulk: Gray Hulk: Gray is a comic book limited series written by Jeph Loeb and illustrated by Tim Sale. # Publication history. The series ran for a total of six issues which followed the early years of Bruce Banner and his problems as the Hulk. Jeph Loeb and Tim Sale also collaborated on other limited seri...
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Hulk: Gray
https://en.wikipedia.org/w/index.php?title=Hulk:%20Gray
Hulk: Gray # Publication history. The series ran for a total of six issues which followed the early years of Bruce Banner and his problems as the Hulk. Jeph Loeb and Tim Sale also collaborated on other limited series such as: "", "", and "". Each series, like "Hulk: Gray", followed the early years of the different Mar...
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2012 Lillestrøm SK season
https://en.wikipedia.org/w/index.php?title=2012%20Lillestrøm%20SK%20season
2012 Lillestrøm SK season 2012 Lillestrøm SK season The 2012 season saw Lillestrøm compete in the Tippeligaen as well as the 2012 Norwegian Football Cup. They finished the season in 9th in the Tippeligaen and they were knocked out of in the Fourth Round by Bodø/Glimt. It was the club's first season with Magnus Haglund...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award Börje Langefors Best Doctoral Dissertation Award The Börje Langefors Award (‘Börje Langeforspriset’ in Swedish) is an academic prize awarded each year by the Swedish Information Systems Academy (Svenska informationssystemakademin or SISA) for the best doctoral dissertat...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award and computer scientist, and emeritus professor of business information systems at the Department of Computer and Systems Science, Stockholm University and Royal Institute of Technology, Stockholm. Börje Langefors was a pioneer of IT and one of the initiators of "informat...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award following quality criteria are applied for the evaluation of individual doctoral thesis: - Relevance: Articulate, well-defined and well-motivated research question(s) - Articulate and well-reflected research design - Comprehensiveness: Chosen and used well described t...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award Ability to serve as a "Role model" - International exposure /review # Prize committee. Every year in spring (usually in May), a prize committee assesses the theses submitted by the universities/institutions in Sweden and nominates the best dissertation, which is final...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award Langefors Awards during the period 2011-2015 was sponsored by Nethouse and Sitevision # The recipients. ## 2015. First prize "Johan Sandberg", Postdoctoral Researcher at the Umeå University awarded the first best for his doctoral dissertation entitled "Digital Capabi...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award on Online Interactivity". The motivation for the award as described by the SISA Börje Langefors Prize Committee : "The thesis is based on a socially relevant contemporary topic, well-designed and well-defined subject area with contrasting perspectives based on exceptiona...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award The motivation for selected him as the first is, as stated by SISA is “Henrik Wimelius dissertation is well written and clearly positioned against existing literature. The question that is addressed both theoretically and practically interesting. Methodologically the res...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award Assistant Professor at the School of Business (Informatics) of Örebro University received the second best award for his dissertation – “Creating Opportunity by Connecting the Unconnected: Deploying Mobile Phone based Agriculture Market Information Service for Farmers in ...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award IT use. The research project was executed through a well-designed process that is presented in a comprehensive yet detailed way. In particular, it reveals how and why certain practical and theoretical issues were tackled to push the project forward. Constituting the core...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award motivation for the award as described by the SISA Börje Langefors Prize Committee : "Annika Andersson is awarded the prize for best dissertation for the following reasons: socially relevant subject matter, well formulated and relevant theoretical basis, proper research d...
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Börje Langefors Best Doctoral Dissertation Award
https://en.wikipedia.org/w/index.php?title=Börje%20Langefors%20Best%20Doctoral%20Dissertation%20Award
Börje Langefors Best Doctoral Dissertation Award Senior Lecturer and Researcher at the Department of Informatics and Media, Uppsala University stood runner-up for the Börje Langefors Award for best Information Systems (IS) thesis in Sweden 2009-2010. The title of his thesis is Designing Information Systems - A Pragmati...
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NA-17 (Haripur)
https://en.wikipedia.org/w/index.php?title=NA-17%20(Haripur)
NA-17 (Haripur) NA-17 (Haripur) NA-17 (Haripur) () is a constituency for the National Assembly of Pakistan. It covers tho whole of district Haripur. The constituency was formerly known as NA-19 (Haripur) from 1977 to 2018. The name changed to NA-17 (Haripur) after the delimitation in 2018. # Detailed results. ## 200...
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NA-17 (Haripur)
https://en.wikipedia.org/w/index.php?title=NA-17%20(Haripur)
NA-17 (Haripur) tailed results. ## 2002 General Election. "A total of 6,277 votes were rejected." ## 2008 General Election. "A total of 5,757 votes were rejected." ## 2013 General Election. "A total of 8,467 votes were rejected." ## 2015 By-election. A by-election took place on 16 August 2015. "A total of 3,91...
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François Gernelle
https://en.wikipedia.org/w/index.php?title=François%20Gernelle
François Gernelle François Gernelle François Gernelle (born December 20, 1944) is a French engineer, computer scientist and entrepreneur famous for inventing the first micro-computer using a micro-processor, the Micral N. # Education. In the late 1960s, Gernelle earned an engineering degree at the Conservatoire Nati...
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François Gernelle
https://en.wikipedia.org/w/index.php?title=François%20Gernelle
François Gernelle on the i8008 development capacity, he resigned in 1972 and joined , a company created and led by Paul Magneron. He designed a micro-computer to answer a request of INRA to measure agricultural hygrometry. During this project, he granted two patents. In 1973 he supported the Micral N and, with the com...
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François Gernelle
https://en.wikipedia.org/w/index.php?title=François%20Gernelle
François Gernelle powered by a poor operating system. In his mind, this kind of poor computer design was a dead-end at a time where really good micro-processors existed and offered capacity to design powerful multi-user and multi-tasking systems at medium and even low cost. ## FORUM International. In 1983, he resigne...
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François Gernelle
https://en.wikipedia.org/w/index.php?title=François%20Gernelle
François Gernelle existed and offered capacity to design powerful multi-user and multi-tasking systems at medium and even low cost. ## FORUM International. In 1983, he resigned from Bull and founded a new company named which will create professional computers powered by . # External References. - This article is ba...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) The Concert for Bangladesh (film) The Concert for Bangladesh is a film directed by Saul Swimmer and released in 1972. The film documents the two benefit concerts that were organised by George Harrison and Ravi Shankar to raise funds for refugees of the Bangladesh Liberation War, and w...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) Jim Horn and Jim Keltner. The film was the final part of Harrison's "pioneering" aid project for the people of former East Pakistan, following his "Bangla Desh" charity single, the UNICEF benefit concerts, and a triple live album of the event credited to "George Harrison and Friends"....
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) and contextual input from then UN secretary-general Kofi Annan, US Fund for UNICEF president Charles Lyons and Live Aid founder Bob Geldof. As with the live album, sales of the DVD release of the film continue to benefit the George Harrison Fund for UNICEF. # Production. Saul Swimme...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) down in front of it. The compromised quality would result in some brutal edits in the released movie – Eric Clapton, for instance, appears to change jackets and guitar part-way through a song (Leon Russell's medley in particular). In an interview accompanying the 2005 DVD release of th...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) performance of "My Sweet Lord" was previewed during his appearance on ABC-TV's "The Dick Cavett Show" on 23 November, but the movie would not be ready for release until the following spring. # Synopsis. The opening of the movie features footage from the New York press conference, hel...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) showing musicians and support crew preparing for the first show. In voiceover, Harrison provides a brief explanation of how the project came together. The concert begins with Harrison taking to the stage alone and addressing the audience, his comment "We've got a good show lined up – ...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) The audience, apparently believing they have heard an entire piece, respond with enthusiastic applause, to which Shankar replies: "Thank you, if you appreciate the tuning so much, I hope you will enjoy the playing more." The four musicians then launch into a fifteen-minute dhun. A bri...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) two other lead guitarists, Eric Clapton and Jesse Ed Davis, the four members of Badfinger on rhythm guitars and tambourine, a six-piece horn section in matching blue patterned shirts, and a small choir of backing vocalists, a few of whom are also playing percussion. Harrison then turns...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) of the words. Harrison then returns to the spotlight for two of his own numbers, the second being "While My Guitar Gently Weeps", at the end of which Clapton clearly struggles on lead guitar next to Harrison's more measured soloing. Before this, Harrison pauses for a few minutes to int...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) semi-acoustic set of four of his songs. Two more numbers from Harrison close the show. When he apparently forgets part of the second verse in "Something", he looks around, seemingly at Russell or Clapton, and grins his way through it. The film concludes with a spirited version of Harr...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) Alla Rakha (tabla), Kamala Chakravarty (tambura) - "Bangla Dhun" (P.D.) – traditional dhun ## George Harrison and band. Performers: George Harrison (vocals, electric and acoustic guitars), Ringo Starr (vocals, drums), Leon Russell (vocals, piano), Billy Preston (vocals, organ), Eric...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) Claudia Linnear, Joe Greene, Dolores Hall, Jeanie Greene, Marlin Greene, Don Preston - "Wah-Wah" - "My Sweet Lord" - "Awaiting on You All" - "That's the Way God Planned It" (Preston) – performed by Billy Preston - "It Don't Come Easy" (Starkey) – performed by Ringo Starr - "Bewar...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) the Sun" – featuring Pete Ham on second acoustic guitar ## Bob Dylan. Performers: Bob Dylan (vocals, acoustic guitar, harmonica), George Harrison (electric guitar, slide guitar, backing vocals), Leon Russell (bass, backing vocals), Ringo Starr (tambourine) - "A Hard Rain's a-Gonna F...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) only on the "Concert for Bangladesh" album. - "Love Minus Zero/No Limit" – written and performed by Dylan; recorded during the afternoon show and included as an extra on the 2005 DVD. - "Hear Me Lord" – written and performed by George Harrison; played following Dylan's five-song set ...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) My Kitchen" – composed by Robert Johnson and performed by Leon Russell (vocals and piano) with George Harrison (lead guitar and backing vocals), Eric Clapton (second lead guitar), Billy Preston (organ), Carl Radle (bass), and Ringo Starr and Jim Keltner (both on drums); recorded during...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) Lennon was among those attending the preview, along with wife Yoko Ono, although he left the cinema during Dylan's segment. The UK release was delayed until 27 July, however,John Pidgeon, ""Bangla Desh"", "NME", 15 July 1972, p. 24; available at Rock's Back Pages (retrieved 15 July 201...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) succeeds." Reviewing the 2005 reissue for BBC Online, Chris Jones described the concert as a "masterclass" in how to carry out a superstar charity benefit. # 2005 DVD release. A two-disc special edition DVD of "The Concert for Bangladesh" was issued in October 2005, with the concert ...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) talks about organising the concert in voiceovers only. Other interviews are with "Rolling Stone" founder Jann Wenner and Live Aid organiser Bob Geldof, both of whom talk of the historic importance of the event, as well as Apple Corps executive Neil Aspinall. Charles Lyons, then preside...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) that he was booked on every airline flight from London to New York City for a week before the shows. After finally boarding a plane, Clapton performed without the benefit of a rehearsal, and "he was brilliant," Harrison says, somewhat generously. Clapton, for his part, recalls the time...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) of the historic day, Sunday, 1 August 1971. Participants in these features include Swimmer, A&M Studios engineer Norm Kinney, former Capitol Records boss Bhaskar Menon, designer Tom Wilkes, photographer Barry Feinstein, and British film mogul David Puttnam. Menon is noticeably more con...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) Now: The First Complete Beatles Discography 1961–1975", Ballantine Books (New York, NY, 1976; ). - Alan Clayson, "George Harrison", Sanctuary (London, 2003; ). - "The Concert for Bangladesh Revisited with George Harrison and Friends" DVD, Apple Corps, 2005 (directed by Claire Ferguso...
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The Concert for Bangladesh (film)
https://en.wikipedia.org/w/index.php?title=The%20Concert%20for%20Bangladesh%20(film)
The Concert for Bangladesh (film) 61–1975", Ballantine Books (New York, NY, 1976; ). - Alan Clayson, "George Harrison", Sanctuary (London, 2003; ). - "The Concert for Bangladesh Revisited with George Harrison and Friends" DVD, Apple Corps, 2005 (directed by Claire Ferguson; produced by Olivia Harrison, Jonathan Clyde...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques Genetic engineering techniques Genetic engineering can be accomplished using multiple techniques. There are a number of steps that are followed before a genetically modified organism (GMO) is created. Genetic engineers must first choose what gene they wish to insert, modify or delete. Th...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques involved in inheritance tools were developed that allowed their direct manipulation. Important advances included the discovery of restriction enzymes and DNA ligases and the development of polymerase chain reaction and sequencing. This allowed the gene of interest to be isolated and then...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques fully developed plant. Common techniques include microinjection, virus-mediated, "Agrobacterium"-mediated or biolistics. Further tests are carried out on the resulting organism to ensure stable integration, inheritance and expression. First generation offspring are heterozygous, requiring...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques and zinc finger nucleases. Since 2009 more accurate and easier systems to implement have been developed. Transcription activator-like effector nucleases (TALENs) and the Cas9-guideRNA system (adapted from CRISPR) are the two most commonly used. They may potentially be useful in gene thera...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques rapid changes in an organisms makeup could be introduced. Hybridization most likely first occurred when humans first grew similar, yet slightly different plants in close proximity. Some plants were able to be propagated by vegetative cloning. Genetic inheritance was first discovered by G...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques and properties of DNA, tools had to be developed that allowed it to be manipulated. In 1970 Hamilton Smiths lab discovered restriction enzymes, enabling scientists to isolate genes from an organism's genome. DNA ligases, which join broken DNA together, were discovered earlier in 1967. By ...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques well as manipulating DNA, techniques had to be developed for its insertion into an organism's genome. Griffith's experiment had already shown that some bacteria had the ability to naturally uptake and express foreign DNA. Artificial competence was induced in "Escherichia coli" in 1970 by ...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques the tumor and adding in novel genes, researchers were able to infect plants with "A. tumefaciens" and let the bacteria insert their chosen DNA into the genomes of the plants. # Choosing target genes. The first step is to identify the target gene or genes to insert into the host organism...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques cheap, easy to grow, clonal, multiply quickly, relatively easy to transform and can be stored at -80 °C almost indefinitely. Once a gene is isolated it can be stored inside the bacteria providing an unlimited supply for research. Genetic screens can be carried out to determine potential ...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques try and identify the gene responsible are called forward genetics. The gene then needs to be mapped by comparing the inheritance of the phenotype with known genetic markers. Genes that are close together are likely to be inherited together. Another option is reverse genetics. This approa...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques likely function of a gene by comparing its sequence to that of well-studied genes from model organisms. The development of microarrays, transcriptomes and genome sequencing has made it much easier to find desirable genes. The bacteria "Bacillus thuringiensis" was first discovered in 1901...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques resistance to the glyphosate herbicide was found, after seven years searching, in bacteria that were surviving in the outflow pipe of a Monsanto RoundUp manufacturing facility. In animals the majority of genes used are growth hormone genes. # Gene manipulation. All genetic engineering p...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques opened, exposing the DNA without causing too much damage to it. The methods used vary depending on the type of cell. Once open, the DNA must be separated from the other cellular components. A ruptured cell contains proteins and other cell debris. By mixing with phenol and/or chloroform, f...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques that simplify the process. ## Gene isolation. The gene of interest must be separated from the extracted DNA. If the sequence is not known then a common method is to break the DNA up with a random digestion method. This is usually accomplished using restriction enzymes (enzymes that cut ...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques is detected then it is possible that the bacteria contains the target gene. If the gene does not have a detectable phenotype or a DNA library does not contain the correct gene, other methods must be used to isolate it. If the position of the gene can be determined using molecular markers...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques Some gels can separate sequences that differ by a single base-pair. The DNA can be visualised by staining it with ethidium bromide and photographing under UV light. A marker with fragments of known lengths can be laid alongside the DNA to estimate the size of each band. The DNA band at th...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques artificially synthesise genes. Some synthetic sequences are available commercially, forgoing many of these early steps. ## Modification. The gene to be inserted must be combined with other genetic elements in order for it to work properly. The gene can be modified at this stage for bett...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques organism it is expressed in, is used to determine which cells are transformed with the new gene. The constructs are made using recombinant DNA techniques, such as restriction digests, ligations and molecular cloning. # Inserting DNA into the host genome. Once the gene is constructed it ...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques is incorporated into somatic cells, the transgene can not be inherited. ## Transformation. Transformation is the direct alteration of a cells genetic components by passing the genetic material through the cell membrane. About 1% of bacteria are naturally able to take up foreign DNA, but...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques chloride partially disrupts the cell membrane, which allows the recombinant DNA to enter the host cell. It is suggested that exposing the cells to divalent cations in cold condition may change or weaken the cell surface structure, making it more permeable to DNA. The heat-pulse is thought...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques the holes are rapidly closed by the cell's membrane-repair mechanisms. Up-taken DNA can either integrate with the bacterials genome or, more commonly, exist as extrachromosomal DNA. In plants the DNA is often inserted using "Agrobacterium"-mediated recombination, taking advantage of the ...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques plant cell nucleus and integrated into the host plants genomic DNA.The plasmid T-DNA is integrated semi-randomly into the genome of the host cell. By modifying the plasmid to express the gene of interest, researchers can insert their chosen gene stably into the plants genome. The only es...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques coated with DNA and then shot into young plant cells or plant embryos. Some genetic material enters the cells and transforms them. This method can be used on plants that are not susceptible to "Agrobacterium" infection and also allows transformation of plant plastids. Plants cells can als...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques used to insert foreign DNA into animal cells is usually called transfection. There are many ways to directly introduce DNA into animal cells "in vitro". Often these cells are stem cells that are used for gene therapy. Chemical based methods uses natural or synthetic compounds to form part...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques can be used as vectors to deliver DNA into cultured animal cells. Positively charged liposomes bind with DNA, while polymers can designed that interact with DNA. They form lipoplexes and polyplexes respectively, which are then up-taken by the cells. Other techniques include using electrop...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques the genetic material is injected into one of them. The oocyte is then implanted in the oviduct of a pseudopregnant animal. Another method is Embryonic Stem Cell-Mediated Gene Transfer. The gene is transfected into embryonic stem cells and then they are inserted into mouse blastocysts that...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques virulent genes are removed from the virus and the target genes are inserted instead. The sequences that allow the virus to insert the genes into the host organism must be left intact. Popular virus vectors are developed from retroviruses or adenoviruses. Other viruses used as vectors incl...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques requirements for successful regeneration. If successful, the technique produces an adult plant that contains the transgene in every cell. In animals it is necessary to ensure that the inserted DNA is present in the embryonic stem cells. Offspring can be screened for the gene. All offsprin...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques will not. By growing the cells in the presence of an antibiotic or chemical that selects or marks the cells expressing that gene, it is possible to separate modified from unmodified cells. Another screening method involves a DNA probe that sticks only to the inserted gene. These markers a...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques DNA sequencing is conducted to confirm that an organism contains the new gene. These tests can also confirm the chromosomal location and copy number of the inserted gene. Once confirmed methods that look for and measure the gene products (RNA and protein) are also used to assess gene expr...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques generally insert the new genetic material randomly within the host genome. This can impair or alter other genes within the organism. Methods were developed that inserted the new genetic material into specific sites within an organism genome. Early methods that targeted genes at certain si...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques alter gene expression patterns. If a vital gene is knocked out it can prove lethal to the organism. In order to study the function of these genes, site specific recombinases (SSR) were used. The two most common types are the Cre-LoxP and Flp-FRT systems. Cre recombinase is an enzyme that...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques in certain cells. This has also been used to remove marker genes from transgenic animals. Further modifications of these systems allowed researchers to induce recombination only under certain conditions, allowing genes to be knocked out or expressed at desired times or stages of developme...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques will be integrated at the targeted site with high efficiency by homologous recombination. There are four families of engineered nucleases: meganucleases, ZFNs, transcription activator-like effector nucleases (TALEN), the CRISPR/Cas (clustered regularly interspaced short palindromic repeat...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques double stranded breaks (base editors). ## Meganucleases and Zinc finger nucleases. Meganucleases were first used in 1988 in mammalian cells. Meganucleases are endodeoxyribonucleases that function as restriction enzymes with long recognition sites, making them are more specific to their ...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques for viral vectorization perspectives. Zinc-finger nucleases (ZFNs), used for the first time in 1996, are typically created through the fusion of Zinc-finger domains and the "Fok"I nuclease domain. ZFNs have thus the ability to cleave DNA at target sites. By engineering the zinc finger do...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques to the code governing the DNA recognition by transcription activator-like effectors (TALE) in 2009 opened the way to the development of a new class of efficient TAL-based gene editing tools. TALE, proteins secreted by the Xanthomonas plant pathogen, bind with great specificity to genes wi...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques standard molecular biology procedure and rely on more complicated method of such as Golden gate cloning. In 2011, another major breakthrough technology was developed based on CRISPR/Cas (clustered regularly interspaced short palindromic repeat / CRISPR associated protein) systems that fu...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques CRISPR/Cas9 could be used to induce double-stranded breaks at specific points within DNA sequences. It was later demonstrating that CRISPR/Cas9 could edit human cells in a dish. Although the early generation lacks the specificity of TALEN, the major advantage of this technology is the sim...
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Genetic engineering techniques
https://en.wikipedia.org/w/index.php?title=Genetic%20engineering%20techniques
Genetic engineering techniques be used to induce double-stranded breaks at specific points within DNA sequences. It was later demonstrating that CRISPR/Cas9 could edit human cells in a dish. Although the early generation lacks the specificity of TALEN, the major advantage of this technology is the simplicity of the des...
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Simon Walker
https://en.wikipedia.org/w/index.php?title=Simon%20Walker
Simon Walker Simon Walker Simon Walker may refer to: - Simon Walker (business) (born 1953), Director General of the Institute of Directors - Simon Walker (composer) (born 1961), Australian composer - Simon Walker (sailor), English yachtsman, adventurer and author - Simon Walker (Hollyoaks), a character on the Brit...
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Cardiff West Yard Locomotive Works
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Cardiff West Yard Locomotive Works Cardiff West Yard Locomotive Works West Yard Works was the Taff Vale Railway's locomotive repair and construction factory. It was located in Cardiff between Bute Street and the Glamorganshire Canal. A small engine shed with room for one locomotive and a repair shop was built there w...
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Cardiff West Yard Locomotive Works
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Cardiff West Yard Locomotive Works ubiquitous across South Wales, the works traverser could not accommodate the longer wheelbase so locomotives had to have their trailing radial wheels removed while within the works. The works could only be accessed by level crossings accessed by turntables on the main line near the r...
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Cardiff West Yard Locomotive Works
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Cardiff West Yard Locomotive Works motors were all built by outside contractors. After the Great War there were plans to build a new works at Radyr but as the company was to amalgamate with the Great Western Railway and other South Wales companies in 1922 that plan was abandoned and the Great Western subsequently conc...
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Cardiff West Yard Locomotive Works
https://en.wikipedia.org/w/index.php?title=Cardiff%20West%20Yard%20Locomotive%20Works
Cardiff West Yard Locomotive Works plans to build a new works at Radyr but as the company was to amalgamate with the Great Western Railway and other South Wales companies in 1922 that plan was abandoned and the Great Western subsequently concentrated all major locomotive repair work in South Wales at the former Rhymney...
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Tales for the Midnight Hour
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Tales for the Midnight Hour Tales for the Midnight Hour Tales for the Midnight Hour is a series of scary children's books written by Judith Bauer Stamper. This anthology horror series served as the precursor to various other similar works, including "Scary Stories to Tell in the Dark" and "Scary Stories for Sleep-over...
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Tales for the Midnight Hour
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Tales for the Midnight Hour Published by Scholastic's Point Horror banner, this popular series spawned 3 sequels and lasted from 1977-1991. # Overview. The series was written for a younger audience, but was told much darker than many books of the time. With the exception of the first volume, each book contained 13 st...
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Arthur Barnwell House
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Arthur Barnwell House Arthur Barnwell House The Arthur Barnwell House, which is also known as the Barnwell-DeCamps House, is a Queen Anne house in Greer, South Carolina that was built in the period 1880-1900. It was named to the National Register of Historic Places on 1992. As of 2013, the house was in the process of ...
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