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Host-tumor relationship XXXIII. Inhibitor of hyaluronidase in blood serum of cancer patients. Inhibiting activity of blood serum was determined from the decrease of N-acetyl-hexosamine end groups of hyaluronic acid products released by testicular hyaluronidase. Maximum inhibition was observed within the region of pH fr... |
Influence of peritoneal dialysis on factors affecting oxygen transport. To determine the effect of changing concentrations of uremic metabolites on factors affecting oxygen transport, without the effects of extracorporeal blood pumping, we studied five patients before, during and after peritoneal dialysis. Significant ... |
Some effects of ammonium salts on renal histology and function in the dog. NH4Cl was infused into the left renal artery of anesthetized dogs at 50-125 mum/kg/min for up to 110 min. Renal blood flow declined early then increased to supra-control levels during infusion. Kidneys perfused at 125 mum/kg/min for 90 min showe... |
Papillary necrosis associated with calyceal arteritis. The renal papilla has a double blood supply - from both the vasa recta and the calyceal arteries. The importance of the latter supply is not established. A case of polyarteritis associated with papillary necrosis is reported, in which the calyceal vessels, supplyin... |
Fasting uric acid and phosphate in urine and plasma of renal calcium-stone formers. Clearance experiments in calcium-stone patients (n = 60) and controls (n = 60) demonstrated significantly higher urinary uric acid (UA) in younger (less 40 years) stone patients than controls (median: 480 vs. 351 mug/min) but not in old... |
Effects of varying dialysate calcium concentrations on the plasma calcium fractions in patients on dialysis. The plasma-ionized calcium levels decreased during haemodialysis when a dialysate calcium concentration of 5 mg/100 ml was used. When dialysis was performed with a bath calcium concentration of 7.5 mg/100 ml, th... |
[Borderline between antiepileptic and psychopharmacological drugs (author's transl)]. From the chemical and pharmalogical point of view it is impossible to separate strictly between antiepileptic and psychopharmalogical drugs. Inspite of that and several clinical overlappings it is possible and helpful finding differen... |
Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Soluble glycoproteins have been purified from a series of clones of Trypanosoma brucei 427. Each clone yielded a characteristic predominant glycoprotein which induced clone-specific i... |
The determination of gamma-glutamyl transpeptidase by reaction rate assay at 37 degrees C. The determination of gamma-glutamyl transpeptidase by a reaction rate assay using optimal reaction conditions at 37 degrees is described. Specific conditions and instrument settings are described for the LKB Reaction Rate Analyse... |
The influence of cortisol on the enzymes of fatty acid synthesis in developing mammalian lung and brain. Administration of cortisol to fetal rabbits resulted in a 42% inhibition of pulmonary de novo fatty acid synthesis from acetyl coenzyme A (CoA) (P = less than 0.025). This was associated with inhibition of acetyl-Co... |
The relation between myosin adenosinetriphosphatase activity and inactivation of myosin under alkaline conditions of heart muscles in mammals of different size. ATPase activity of myosin in the heart muscle of the mouse, rat, guinea-pig, rabbit and pig was studied at neutral pH and under mild alkaline conditions. At ne... |
Carbon dioxide response curves during hypothermia. The responsiveness of the medullary chemoreceptors, measured by the ventilatory response to hypercapnia given in an hyperoxic gas mixture in intact anesthetized dogs has been evaluated during normothermia and at two levels of hypothermia. The response was studied in: 1... |
[Zollinger-Ellison syndrome treated medically by an inhibitor of H2 histamine receptors]. Metiamide an histamine H2-receptors antagonist has been used to treat a case of Zollinger-Ellison syndrome characterized by a long standing diarrhea, an important gastric hypersecretion and a moderatly elevated plasma gastrin but ... |
[Exercise test in the asthmatic patient. Study of 75 patients]. Frequency of exercise-induced asthma has been studied in 75 unselected asthmatic patients (adults and children) by measuring the forced expiratory volume at one second (FEV1) and the vital capacity (VC) before and after a treadmill exercise, continued unti... |
[Anaphylactic accidents due to glaphenine. 5 cases]. The authors report 5 cases of shock type anaphylactic reactions after the ingestion of glafenine. Up to the present, acute tubulo-interstitial nephritis had chiefly been reported. The existence of allergy to this medication remains to be proved by the wider applicati... |
Intranasal betamethasone valerate in seasonal rhinitis. A double-blind study comparing betamethasone valerate and placebo aerosols was carried out in 40 patients with a history of seasonal allergic rhinitis and positive skin tests to grass pollens. Analysis of the symptoms recorded on a daily record card for a period o... |
Urinary acidification in renal allografts. Urinary acid excretion was measured in 35 human renal allograft recipients during the first few days after the onset of diuresis and two months after transplantation. In 16 out of 17 rejection episodes an early significant decrease of renal total net H+ excretion was observed.... |
A simple test for early detection of severe renal homograft rejection. Adding urine to a standard buffered fibrinogen solution and then coagulating it with thrombin gives reproducible coagulation times with normal urines. Coagulation of fibrinogen by thrombin is prolonged in acid solutions with a pH below six. Urines o... |
Catalysis by acetylcholinesterase: evidence that the rate-limiting step for acylation with certain substrates precedes general acid-base catalysis. Inferences about the catalytic mechanism of acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) are frequently made on the basis of a presumed analogy with chymotryp... |
Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes. The light-induced absorbance change at 518 nm of isolated chloroplasts consists of a rapid phase, and a slow phase which is complete in about 20 sec. The slow component of the 518 nm absorbance change correlates wi... |
phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli. Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. ... |
Intramolecular arsanilazotyrosine-248-Zn complex of carboxypeptidase A: a monitor of catalytic events. The intensely chromophoric intramolecular coordination complex formed between arsanilazotyrosine-248 and the active site zinc atom of azocarboxypeptidase A (Johansen, J. T. & Vallee, B. L. (1971) Proc. Nat. Acad. Sci.... |
Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Escherichia coli. Addition of ubiquinone-1 to E. coli ML 308-225 membrane vesicles dramatically increases coupling between NADH oxidation and active transport such that initial rates and steady-state levels of lactose and a... |
Protection of lethally irradiated mice with allogeneic fetal liver cells: influence of irradiation dose on immunologic reconstitution. After lethal irradiation long-lived, immunologically vigorous C3Hf mice were produced by treatment with syngeneic fetal liver cells or syngeneic newborn or adult spleen cells. Treatment... |
Mechanism of action of penicillin: triggering of the pneumococcal autolytic enzyme by inhibitors of cell wall synthesis. During penicillin treatment of an autolysin defective mutant pneumococcus we have observed three novel phenomena: (i) Growth of the mutant cultures is inhibited by the same concentrations of penicill... |
Identification of NADPH-thioredoxin reductase system in Euglena gracillis. Euglena gracilis contains a protein system which can utilize the reducing power of NADPH in the ribonucleotide reductase-catalyzed reduction of CTP. The proteins required for this reaction are a flavoprotien with a molecular weight of approximat... |
Purification of folate binding factor in normal umbilical cord serum. Human umbilical cord serum was found to contain both free folate and folate complexed to a high-molecular weight factor. The complexed folate was bound to a very high affinity binder and was present in concentrations equivalent to as much as 60 ng of... |
Intramolecular arsanilazotyrosine-248-Zn complex of carboxypeptidase A: a monitor of multiple conformational states in solution. The red azoTyr-248-Zn complex of arnilazocarboxypeptidase, previously used to demonstrate differences in conformation of the enzyme in crystals and in solution, has now provided means to dete... |
Presence of norepinephrine and related enzymes in isolated brain microvessels. Norepinephrine and the enzymes involved in its synthesis and degradation were found to be associated with isolated brain microvessels. The significance of these results are discussed with respect to adrenergic innervation of the cerebral mic... |
Disorders of micturition in bacterial prostatitis. Measurements of urinary flow rate were performed in 16 patients with established prostatitis before and after a course of antimicrobial therapy. Before treatment the maximum flow rates were poor with abnormal flow curves and significant improvement in voiding character... |
Nonenzymatic reactivation of des-acetyl citrate lyase by acetyl adenylate. First example of enzyme activation by chemotrophic modification. The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 MM acetyl AMP causes a fast rea... |
Relationship between bovine serum albumin structure and its chemical equilibria with hydrogen and 5-hydroxytryptamine ions. From the analysis of titration curves with hydrogen and 5-HT ions, it was found that the electrostatic interaction parameter of protein macroion and the number sites of 5-HT fixing were smaller ov... |
Some psysicochemical properties of mitochondrial and cell sap alanine aminotransferase from the rat CNS. The authors describe different properties of brain mitochondrial and cell sap alanine aminotransferase. They showed that the mitochondrial enzyme was inhibited by maleate, chlorides, acetate and phosphate with a hig... |
[Postvacccinal encephalomyelitis after protective antirabic innoculation, combined with polioencephalomyelitis (lyssa?)]. By means of two cases of postvaccinal encephalomyelitis following antirabies vaccination has been demonstrated the meaning of these complications. In one of these cases has been presumed a simultane... |
The effect of L-dopa on young patients with simple schizophrenia, treated with neuroleptic drugs: a double-blind cross-over trial with Madopar and placebo. Thirteen out of 18 young out-patients with simple schizophrenia under neuroleptic treatment completed a double-blind cross-over trial with Madopar [L-Dopa + bensera... |
The specificity of binding of the narcotic agonist etorphine in synaptic membranes of rat brain in vivo. When 3H-etorphine was administered to rats in a pharmacologically effective dose (0.75 mug/kg intracisternally), the labeled drug was concentrated in synaptic membrane fractions isolated from the brains of rats kill... |
The cholinergic system and nociception in the primate: interactions with morphine. In Experiment 1 the shock titration task was used to evaluate the antinoceptive properties of 5 different classes of cholinergic compounds in the rhesus monkey. Only scopolamine and high doses of physostigmine were effective in elevating... |
Effects of thienodiazepine derivatives on human sleep as compared to those of benzodiazepine derivatives. The effects of new thienodiazepine derivatives, such as clotiazepam and Y-7131, on normal human sleep were investigated on 5 subjects and compared to those of benzodiazepine derivatives, such as diazepam and nitraz... |
Effect of two weeks' treatment with thioridazine, chlorpromazine, sulpiride and bromazepam, alone or in combination with alcohol, on learning and memory in man. Forty paid healthy male students participated in two subacute experiments of 6 weeks each. In the first trial 20 of them received bromazepam, thioridazine, and... |
Effects of chronic exposure to stressors on avoidance-escape behavior and on brain norepinephrine. A single exposure to a severe stressor (either cold swim or inescapable shock) impairs subsequent performance in a shuttle avoidance-escape task (1), a deficit attributed to reduction in brain noradrenergic activity produ... |
Evaluation of combined pharmacological and psychotherapeutic treatment in patients with functional abdominal disorders. 78 patients suffering from various functional abdominal complaints have been trated in a 2 x 2 double-blind design: (a) psychotherapy with Ro 5-3350 (TH/Ro); (b) psychotherapy with placebo (TH/P); (c)... |
Role of cardiovascular and ionic changes in pathogenesis and prevention of isoprenaline-induced cardiac necrosis. Blood pressure, heart rate, oxygen uptake, and blood values of PO2, PCO2, and pH were studied in unanesthetized rats for 8 hours. After a cardiotoxic dose of 20 mg/kg isoprenaline, s.c., blood pressure fell... |
Alterations in norepinephrine pattern in the damaged myocardium in the rat. In the albino rat, the evolvement of myocardial necrosis induced by a single injection of ISO was accompanied by a fall in total NE. Pretreatment with propranolol and pargyline protected against ISO-induced necrosis and myocardial hypertrophy, ... |
Prevention of myocardial Ca overload and necrotization by Mg and K salts or acidosis. The crucial point in the pathogenesis of isoproterenol-induced myocardial necrotization is an abundant intracellular Ca accumulation leading to high energy phosphate exhaustion. Accordingly, in the early stage of the isoproterenol-ind... |
Explanation of the stimulation of microsomal N-demethylation reactions by soluble supernatant fraction. Addition of the cell soluble supernatant fraction to an assay medium containing NADPH generating system, mixed function oxidase substrate and microsomes, resulted in a stimulation of drug metabolism ranging from 12-7... |
An attempt to correlated analgesia to changes in brain neuromediators in rats. The analgesic activity of delta9THC, morphine and sodium salicylate was studied concomitantly with changes in brain stem levels of 5HT, 5HIAA, DA and NA. The results show that a correlation exists between analgesia and changes in the seroton... |
Colorimetric determination of 5-aminosalicylic acid and its N-acetylated metabolite on urine and feces. A simple and convenient colorimetric method is described for the quantitative determination of 5-aminosalicylic acid (5-ASA) and N-acetyl-5-ASA in urine and feces after oral administration of salicylazosulfa-pyridine... |
[Pressures, blood gases, pH, lactate and pyruvate concentrations in the portal venous blood in patients after laparotomy during the first 9 days (author's transl)]. In 24 patients (16 women, 8 men, mean age 60 years), who underwent abdominal operations and who had a uncomplicated postoperative course, a teflon-tube was... |
Biochemical and biophysical changes in guinea pigs after acute head injury. Animal experiments were set up mainly to derive additional diagnostic data from the study of biochemical changes after acute head injury. In standardized experiments guinea pigs were subjected in groups of 20 to three identical head injuries, e... |
Total brain ischaemia in dogs: cerebral physiological and metabolic changes after 15 minutes of circulatory arrest. Cross-clamping of the ascending aorta in dogs for 15 min produced severe neurological deficit, observed for up to 20 h. Immediately after restoration of the circulation, the intracranial pressure in the c... |
Oxygen and carbon dioxide dissociation of duck blood. Oxygen and CO2 dissociation of duck blood was studied in blood samples equilibrated with known gas mixtures at the bird's body temperature (41 degrees C) and analyzed in the Van Slyke manometric apparatus and in pH electrodes. At various pH values between 7.38 and 7... |
First apparent dissociation constant of carbonic acid, pK'1, of plasma and erythrocytes. The first apparent dissociation constant of carbonic acid, pK'1, of plasma and red cells was determined on venous blood of ten healthy, adult, male, human subjects. pH and PCO2 of plasma and red cells were analyzed electrometricall... |
The effects of changes in pH and PCO2 in blood and water on breathing in rainbow trout, Salmo gairdneri. The effect of sustained hypercapnia on the acid-base balance and gill ventilation in rainbow trout, Salmo gairdneri, was studied. The response to an increase in PICO2 from 0.3 to 5.2 mm Hg was a five-fold increase i... |
The effect of pH upon fluoride uptake in intact enamel. The relationship between pH and fluoride uptake in intact enamel of permanent premolars was investigated by using: (1) a sodium fluoride dentifrice, (2) a potassium fluoride + manganese chloride dentifrice, and (3) a sodium fluoride solution of the same fluoride c... |
Determination of inorganic pyrophosphatase in rat odontoblast layer by a radiochemical method. The enzyme inorganic pyrophosphatase (PPiase, EC 3.6.1.1) from the odontoblastic layer of rat incisors has been studied by means of a radiochemical micromethod. The enzyme was incubated with 32P-pyrophosphate in tris-HCl buff... |
Assessment of denture plaque pH in subjects with and without denture stomatitis. To evaluate the "resting" pH and induced pH changes in denture plaque, soft deposits were collected from the fitting surface of the denture, pooled and suspended in water. Plaque pH was determined with microelectrode equipment before and a... |
Bioluminescence assay of enzymes obtained from buccal epithelium by superficial scraping. A method is presented for the simultaneous assay of buccal enzymes by measuring reduced nicotinamide adenine dinucleotide and adenosine triphosphate with the aid of the bioluminescence of luciferase extracts. The activity of gluco... |
Allogeneic marrow transplantation for the treatment of leukaemia. A review. 22 HL-A antigen and mixed leukocyte culture-matched sibling bone marrow transplants were attempted in patients with acute leukaemia (at the National Cancer Institute) to define the toxicities of four different immunosuppressive regimens, the co... |
Current status of treatment of pneumonia. Proper treatment of pneumonia is dependent upon a correct diagnosis. Pneumonia may be due to infectious agents, allergic phenomena, or chemical causes. Treatment regimens are outlined for the various types of pneumonia--pneumococcal, staphylococcal, fungal, and pneumonia due to... |
'Picture frame' fibres in a carrier of the trait for malignant hyperpyrexia. A member of a family which was known to be susceptible to malignant hyperpyrexia, who was identified as a carrier by the presence of an elevated serum creatine-phosphokinase, has been investigated further. Muscle was examined biochemically, an... |
The GP dilemma. Recommendations and synopsis of a student conference. The proceedings of a conference organised by students are reported. The present standing of the general practitioner and his need in different societies are equated and the obvious deficiencies are considered. Such themes as maldistribution, service ... |
Gastric emptying of liquids after different vagotomies and pyloroplasty. Gastric emptying of five liquid meals which differ in their physicochemical properties have been measured in control dogs and dogs that have received a Heinecke-Mikulicz pyloroplasty alone, proximal gastric vagotomy without drainage, selective gas... |
Pancreatic enzyme response with an elemental diet. Elemental diets can maintain or slightly improve the nutritional status without a major stimulatory effect on the pancreas. Six dogs were maintained with a regular chow diet, switched to an elemental diet and, subsequently, returned to a chow diet. Cannulation of the p... |
Gastric fibrinolysis. Gastric juice from 15 normals, 20 patients with gastric ulcer and 14 patients with erosive haemorrhagic gastroduodenitis was investigated in respect of its activity on unheated and heated fibrin plates and its content of FDP and plasminogen or plasmin with immunochemical methods. Gastric juice fro... |
Acid-base parameters in the dehydrated camel. The effect of prolonged (10 days) dehydration on acid-base parameters of camel blood was examined. The pH and PCO2 levels rose significantly in the course of dehydration. This state was comparable with compensated non-respiratory alkalosis found in other animals. The plasma... |
[Clinical experiences with untreated homologous vein grafts in reconstruction of arteries (author's transl)]. 50 transplantations of homologous vein grafts in reconstruction of arteries are reported on. Vein grafts were either transplanted immediately or used after deep freezing. This procedure has proved to be effecti... |
The effect of a simulated subarachnoid hemorrhage on cerebral blood flow in the monkey. The hydrogen clearance method was used to measure local and total cerebral blood flow (CBF) in the rhesus monkey before and for five hours after a simulated subarachnoid hemorrhage (SAH). CBF remained stable after SAH unless SAH was... |
Effects of oxygen saturation and pCO2 on brain uptake of glucose analogues in rabbits. The effect of oxygen saturation and PCO2 on brain uptake of glucose analogues was studied in rabbits. Using a modified Oldendorf technique, 14C-labeled glucose analogues with a 3H2O reference standard were introduced into the cerebra... |
Engraftment of allogeneic dog bone marrow. Resistance to allogeneic bone-marrow grafts (AR) was found to occur in many species, including the dog. The i.v. administration of silica particles suppressed AR in vivo in this species. Genetic studies provide suggestive evidence for the existence of a previously unrecognized... |
Lactate and pyruvate concentrations, and acid-base balance of cerebrospinal fluid in experimentally induced intracerebral and subarachnoid hemorrhage in dogs. The effect of blood injected into either subarachnoid space or subcortical brain tissue upon lactate and pyruvate concentrations as well as acid-base balance of ... |
A study of variables affecting the quality of platelets stored at "room temperature". The effect of variables associated with the donor and with methods of collecting, processing, and storing platelets on the quality of platelets kept at ambient temperature was studied. Changes in structural integrity of platelets, dec... |
Dopamine-containing neurons of the substantia nigra and their terminals in the neostriatum. Tne ultrastructural and fluorescence histochemical characteristics of the mature rabbit substantia nigra and neostriatum have been reviewed as a frame of reference for the developmental study. Biochemical investigations were rep... |
[Separation of the hormones of the adenohypophysis of rats by use of electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate]. A comparative study of rat adenohypophysis extract and its alcohol fractions was performed by two variants of the method of electrophoresis in polyacrylamide gel: at pH ... |
[Properties of NAD-glycohydrolase of the nuclei of the liver cells of rats]. Certain properties of the rat liver cell nuclei NAD-glycohydrolase (EC 3.2.2.5) were investigated. It is established that its highest activity is at 37 degrees with activation energy equal to 9480 cal/M and with factor Q10 equal to 1.5. The en... |
[Some properties of "soluble" Na+ and K+-ATPase obtained from various subcellular membrane structures of the brain by use of non-ionic detergents]. A comparative study was carried out of some properties of "soluble" Na+, K+-ATPase obtained from different subcellular membrane brain structures by means of non-ionic deter... |
[Properties of glutamine synthetase of the brain of rats during ontogenesis]. When investigating activity of glutamine synthetase of the enzymatic preparations isolated from the brain of rats of 0.5, 1, 3, 12 and 24-month age, no considerable differences were found in the indices of the values Km to a-glutamate and Vma... |
[Properties of NAD-pyrophosphorylase of the nuclei of liver cells of chickens]. Certain characteristics of chicken liver cells nuclei NAD-pyrophosphorylase (NMN-adenylytranspherase, EC 2.7.7.1) were investigated. It was established that NAD-pyrophosphorylase activity optimum pH is within interval of 7.0-7.5; temperatur... |
[Biosynthesis of flavins and its regulation in the yeast Pichia guilliermondii]. The nature of riboflavin precursors was studied in the yeast Pichia guilliermondii. By means of mutants with blocked GMP-synthetase the purine precursors of riboflavin were shown to belong to guanylic compounds. Accumulation of 2,4,5-triam... |
[DNases and RNases of Misgurnus fossilis ovocytes]. The pH optima were determined for DNases and RNases of the loach eggs. For DNases they are 5.6 and 7.6 and for RNases - 5.2 and 7.2. It is established that Ca++ activates, and Fe++ has not effect on the activity of acid and alkaline DNases, while Mg++, Mn++ and especi... |
[Determination of optimal conditions for the electron-cytochemical detection of ATPase activity in isolated nuclei]. The optimal conditions are selected for electron-cytochemical detection of the ATPase activity in nuclei of the skeletal muscles of rabbits and nuclei of Vicia faba L. meristem. It is shown that the prev... |
[Catecholamines, cholinergic and serotoninergic complexes as criteria of prognosis in acute cranio-cerebral trauma]. In experiments on 30 animals and in observations over the course of acute closed craniocerebral trauma in 24 patients is was found that the course and prognosis of a posttraumatic period were dependent o... |
[Incarcerated gallbladder in cholecystitis]. Among 206 examined patients with cholecystitis and similar diseases in 112 (65%) cases, as evidenced by the authors' findings, the gallbladder proved to be non-functioning. Its escape in the biliary system was functional (indirect) or organic (absolute). The main causes of a... |
[Determination of the proteolytic activity of beef liver by means of natural substrates labed with 125 I]. There is a description of the determination of the enzymatic activity of acid proteinases: the method is based on the use of 125J-labelled natural protein substrates. Labelled albumin 125J, globulin 125J, and insu... |
Significance of serum pepsinogen and abomasal pH levels in a field infection of O circumcincta in lambs. Serum pepsinogen estimations from serially bled lambs grazing on pasture from spring to autumn showed correlations with the availability of Ostertagia larvae on pasture, with faecal egg counts of O circumcincta, and... |
[Cultivation of Trichomonas gallinarum and Trichomonas tenax on a trimed medium]. Studied was the possibility to isolate and culture Tr. gallinarum and Tr. tenax on a medium Trimed, proposed by the authors for the isolation and cultivation of Tr. vaginalis. The growth and development of the two Trichomonas species were... |
Amylase of the thermophilic actinomycete Thermomonospora vulgaris. alpha-Amylase of the thermophilic actinomycete Thermomonospora vulgaris was partially purified. Maximal enzyme activity was obtained at 60degreeC and pH 6.0. KM value was l.4%. The effect of some metal salts on enzyme activity was studied. Enzyme activi... |
[The effect of therapeutic gentamycin doses on the enzyme secretion in urine]. 8 patients with chronic pyelonephritis were given gentamycin intramuscularly injected in individual dosage during 8-10 days. Here the behaviour of the excretion of protein, alanine aminopeptidase alkaline phosphatase, alpha-glucosidase, gamm... |
[Alkaline phosphatases in human feces, intestinal mucosa and bile, and the occurrence of 5'-nucleotidase in feces (author's transl)]. Alkaline phosphatase (EC 3.1.3.1) in extracts of human feces resembles alkaline phosphatase in extracts of duodenal mucosa, except for its electrophoretic mobility in starch gel. It is v... |
Simultaneous determination of 5'-nucleotidase and alkaline phosphatase activities in serum. A simple method is described for the simultaneous determination of alkaline phosphatase (EC 3.1.3.1) and 5'-nucleotidase (EC 3.1.3.5) in serum. The method is based on the determination of inorganic phosphorus released by the act... |
Absorption of short and medium chain fatty acids in the jejunum of the rat. The uptake of the shortest six fatty acids (acetic to octanoic) was studied in vitro, using everted segments of rat jejunum. The marked influence of medium-pH and fatty acid chain-length suggests that non-ionic diffusion through the lipoid memb... |
Carcinogenic N-nitro-dimethylamine from the reaction of the analgesic amidopyrine and nitrite extracted from foodstuffs. The reaction of the analgesic amidopyrine (100 mg) with nitrite extracted from cured meats and from spinach in varying degrees of spoilage was studied. Unde physiological conditions the carcinogenic ... |
[A modern quick method for the acidity determination of the gastric juice (pentagastrin test)]. The author describes a very rapid and accurate method of determining the acidity of gastric juice which is based on recent findings. Starting from the electrometrically determined pH value and the volume of the gastric juice... |
Histochemical observations on the occurrence of glycolytic and pentose phosphate cycle enzymes in the hepatopancreas and their possible relation to eyestalk factor(s) in the crab Scylla serrata (Forskal). Histochemical studies were carried out on some of the glycolytic enzymes viz. phosphorylase, aldose, alpha-glycerop... |
[Acid-base equilibrium during physiological pregnancy]. Authors applied blood-gas analysis in 30 healthy gravid women on 3,3 occassions making out 90 cases altogether by blood samples taken from the pulp of the finger capillaries. As a control 27 healthy non-gravid women were examined for comparative analysis. In 10 ca... |
[The cathode bound group antigen of dysentery-provoking escherichieae (author's transl)]. Antigens from disrupted cells of dysentery-provoking and of non-enteropathogenic Escherichieae were submitted to immunoelectrophoresis on cellulose acetate stripes at pH 8.0. Among 6 immune sera produced for this purpose by immuni... |
[Isolation of the individual structural elements of bacteria of the genus Bordetella and a study of their properties. I. The formation of mureinoplasts and true protoplasts from B. pertussis]. B. pertussis suspension was tested by De Voe et al. method (1970) and its modification with the solutions of a definite ionic c... |
[Sympathico-adrenal system activity in a primary immune response]. Experiments were carried out on linear mice immunized with sheep erythrocytes; it was found that the primary immune respose developed against the background of significant changes in the state of the sympathico-adrenal system, whose activity was determi... |
[Covalent binding of peroxidase to CH- and AH-Sepharose 4 B]. The properties of peroxidase insolubilized by covalent binding to CH- and AH-Sepharose 4 B in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) are described. CH-Sepharose 4 B bound peroxidase yields an enzyme preparation with a residual s... |
Aerobacter aerogenes PRL-R3 urease. Purification and properties. A method for the preparation of a 150-fold purified and homogenous A. aerogenes urease is reported. The enzyme exhibited two pH optima at pH 7.0 and 7.5 in triethanolamine and phosphate buffer, respectively. The affinity of the enzyme toward its substrate... |
Further characterization of the association of glyceraldehyde-3-phosphate dehydrogenase with reticulocyte membranes. 1. The behaviour and properties of membrane-bound GAPDH of rabbit reticulocytes were investigated. 2. The bound GAPDH is more resistant to inactivation by KCl than the soluble enzyme (allotopy). 3. The b... |
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