input stringlengths 50 466 | output dict | schema listlengths 0 0 |
|---|---|---|
Octamer-binding proteins from B or HeLa cells stimulate transcription of the immunoglobulin heavy-chain promoter in vitro. | {
"entities": {
"cell_line": [
{
"text": "B or HeLa cells",
"start": 30,
"end": 45
}
]
}
} | [] |
Extracts of the B-cell line, BJA-B, contain high levels of NF-A2 and specifically transcribe Ig promoters. | {
"entities": {
"cell_line": [
{
"text": "B-cell line",
"start": 16,
"end": 27
},
{
"text": "BJA-B",
"start": 29,
"end": 34
}
]
}
} | [] |
In contrast, extracts from HeLa cells transcribed the Ig promoter poorly. | {
"entities": {
"cell_line": [
{
"text": "HeLa cells",
"start": 27,
"end": 37
}
]
}
} | [] |
Studies with inhibitors of protein synthesis indicated that the time of synthesis of the activator of the IL-2 gene in Jurkat T cells corresponds to the time of appearance of NFAT-1. | {
"entities": {
"cell_line": [
{
"text": "Jurkat T cells",
"start": 119,
"end": 133
}
]
}
} | [] |
Characterization of thyroid hormone receptors in human IM-9 lymphocytes. | {
"entities": {
"cell_line": [
{
"text": "human IM-9 lymphocytes",
"start": 49,
"end": 71
}
]
}
} | [] |
As a first step towards understanding the mechanism of thyroid hormone action in man we have characterized T3 binding sites in nuclei of the human lymphoblastoid line, IM-9 cells. | {
"entities": {
"cell_line": [
{
"text": "human lymphoblastoid line",
"start": 141,
"end": 166
},
{
"text": "IM-9 cells",
"start": 168,
"end": 178
}
]
}
} | [] |
These results suggest that the T3 binding sites present in human IM-9 lymphocyte nuclei and extracts thereof are thyroid hormone receptors. | {
"entities": {
"cell_line": [
{
"text": "human IM-9 lymphocyte",
"start": 59,
"end": 80
}
]
}
} | [] |
Further studies show that genes governing the expression of class II antigens fall into at least three complementation groups; two of these were previously unidentified in mutant cell lines generated in vitro. | {
"entities": {
"cell_line": [
{
"text": "mutant cell lines",
"start": 172,
"end": 189
}
]
}
} | [] |
Though the mutation in at least one mutant line generated in vitro (RJ2.2.5) affects products functioning via interaction with the X box, clear alterations in either NFX1.1 or NFX1.2 are not found in any of the mutant cell lines. | {
"entities": {
"cell_line": [
{
"text": "mutant line",
"start": 36,
"end": 47
},
{
"text": "RJ2.2.5",
"start": 68,
"end": 75
},
{
"text": "mutant cell lines",
"start": 211,
"end": 228
}
]
}
} | [] |
Properties of glucocorticoid receptors in Epstein-Barr virus-transformed lymphocytes from patients with familial cortisol resistance. | {
"entities": {
"cell_line": [
{
"text": "Epstein-Barr virus-transformed lymphocytes",
"start": 42,
"end": 84
}
]
}
} | [] |
In a previous report of two patients with familial glucocorticoid resistance due to reduced numbers of glucocorticoid receptors (GR), we have shown decreased numbers of GR in peripheral mononuclear cells and cultured fibroblasts but normal affinity of GR in both patients. | {
"entities": {
"cell_line": [
{
"text": "cultured fibroblasts",
"start": 208,
"end": 228
}
]
}
} | [] |
Reduced numbers and normal affinity of GR were found in the Epstein-Barr virus-transformed lymphocytes from both patients while the son and daughter had normal numbers and affinity of GR. | {
"entities": {
"cell_line": [
{
"text": "Epstein-Barr virus-transformed lymphocytes",
"start": 60,
"end": 102
}
]
}
} | [] |
These abnormal properties of GR (reduced numbers of GR) were preserved in the transformed cells from the patients. | {
"entities": {
"cell_line": [
{
"text": "transformed cells",
"start": 78,
"end": 95
}
]
}
} | [] |
From several findings, including the absence of OTF-2B (but not OTF-2A) from a lymphocyte line that can not respond to the IgH enhancer, we propose a role of the novel octamer factor in the long range activation by the IgH enhancer. | {
"entities": {
"cell_line": [
{
"text": "lymphocyte line",
"start": 79,
"end": 94
}
]
}
} | [] |
Inhibition of interleukin 2 -induced proliferation of cloned murine T cells by glucocorticoids. | {
"entities": {
"cell_line": [
{
"text": "cloned murine T cells",
"start": 54,
"end": 75
}
]
}
} | [] |
The ability of glucocorticoids to inhibit interleukin 2 (IL 2) -induced T cell proliferation in two cytotoxic T cell (CTL) clones has been studied. | {
"entities": {
"cell_line": [
{
"text": "cytotoxic T cell (CTL) clones",
"start": 100,
"end": 129
}
]
}
} | [] |
A complete inhibition of DNA synthesis by dexamethasone (Dx) could be observed when IL 2-depleted cultures of CTL were either incubated for 6 h with the hormone prior to the addition of IL 2 or treated simultaneously with Dx and a low concentration of IL 2. | {
"entities": {
"cell_line": [
{
"text": "IL 2-depleted cultures",
"start": 84,
"end": 106
},
{
"text": "CTL",
"start": 110,
"end": 113
}
]
}
} | [] |
Furthermore, supernatant from Dx-treated CTL contained a nondialyzable factor which inhibited DNA synthesis and cell growth of CTL clones induced by IL 2. | {
"entities": {
"cell_line": [
{
"text": "CTL",
"start": 41,
"end": 44
},
{
"text": "CTL clones",
"start": 127,
"end": 137
}
]
}
} | [] |
The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa cells (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. | {
"entities": {
"cell_line": [
{
"text": "Namalwa cells",
"start": 162,
"end": 175
},
{
"text": "human Burkitt lymphoma cells",
"start": 177,
"end": 205
}
]
}
} | [] |
Decreased deoxyribonucleic acid binding of glucocorticoid-receptor complex in cultured skin fibroblasts from a patient with the glucocorticoid resistance syndrome. | {
"entities": {
"cell_line": [
{
"text": "skin fibroblasts",
"start": 87,
"end": 103
}
]
}
} | [] |
To explain the end-organ resistance to cortisol, the glucocorticoid receptors (GR) in peripheral mononuclear leukocytes and cultured skin fibroblasts from a forearm skin biopsy were characterized and compared with the results of similar studies in normal subjects. | {
"entities": {
"cell_line": [
{
"text": "cultured skin fibroblasts",
"start": 124,
"end": 149
}
]
}
} | [] |
In the cytosol of cultured skin fibroblasts from the patient, there was also decreased binding capacity. | {
"entities": {
"cell_line": [
{
"text": "cultured skin fibroblasts",
"start": 18,
"end": 43
}
]
}
} | [] |
The thermal stability and the sedimentation coefficient in a sucrose density gradient of the receptors in the cytosol of cultured skin fibroblasts from the patient and normal subjects were similar. | {
"entities": {
"cell_line": [
{
"text": "cultured skin fibroblasts",
"start": 121,
"end": 146
}
]
}
} | [] |
GR complex activation, analyzed by DEAE-cellulose chromatography, was decreased in the patient. | {
"entities": {
"cell_line": [
{
"text": "GR complex activation",
"start": 0,
"end": 21
},
{
"text": "DEAE-cellulose chromatography",
"start": 35,
"end": 64
}
]
}
} | [] |
We show that 1, 25-dihydroxyvitamin D3 (1, 25 [OH] 2D3), the most hormonally active metabolite of vitamin D3, modulates sensitively and specifically both the protein and messenger RNA accumulation of the multilineage growth factor granulocyte-macrophage colony-stimulating factor (GM-CSF). | {
"entities": {
"rna": [
{
"text": "messenger RNA",
"start": 170,
"end": 183
}
]
}
} | [] |
The regulation of GM-CSF expression is seen in both normal human mitogen-activated T lymphocytes and T lymphocytes from a line (S-LB1) transformed with human T cell lymphotropic virus 1 (HTLV-1). | {
"entities": {
"cell_line": [
{
"text": "T lymphocytes from a line (S-LB1) transformed with human T cell lymphotropic virus 1",
"start": 101,
"end": 185
}
]
}
} | [] |
In contrast, cells from a HTLV-1 transformed T lymphocyte line (Ab-VDR) established from a patient with vitamin D-resistant rickets type II with undetectable 1, 25 (OH) 2D3 cellular receptors are resistant to the action of 1, 25 (OH) 2D3. | {
"entities": {
"cell_line": [
{
"text": "HTLV-1 transformed T lymphocyte line",
"start": 26,
"end": 62
},
{
"text": "Ab-VDR",
"start": 64,
"end": 70
}
]
}
} | [] |
The effects of cortisol on the natural killer (NK) activity of human peripheral blood mononuclear (PBM) cells were studied in vitro using a direct 4-h 51Cr-release assay and K 562 cell line as a target. | {
"entities": {
"cell_line": [
{
"text": "K 562 cell line",
"start": 174,
"end": 189
}
]
}
} | [] |
Octamer-containing fragments have been reported to bind a factor present in nuclear extracts of human cell lines; however, identical binding activity was detected in both B lymphoid and non-lymphoid cells. | {
"entities": {
"cell_line": [
{
"text": "human cell lines",
"start": 96,
"end": 112
},
{
"text": "B lymphoid and non-lymphoid cells",
"start": 171,
"end": 204
}
]
}
} | [] |
The promoters of the adenovirus 2 major late gene, the mouse beta-globin gene, the mouse immunoglobulin VH gene and the LTR of the human T-lymphotropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line)... | {
"entities": {
"cell_line": [
{
"text": "HeLa",
"start": 259,
"end": 263
},
{
"text": "CESS",
"start": 265,
"end": 269
},
{
"text": "Epstein-Barr virus-transformed human B cell line",
"start": 271,
"end": 319
... | [] |
LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. | {
"entities": {
"cell_line": [
{
"text": "MT-2",
"start": 54,
"end": 58
}
]
}
} | [] |
Interestingly, a factor showing similar binding specificity to IgNF-A is also present in human HeLa cells. | {
"entities": {
"cell_line": [
{
"text": "human HeLa cells",
"start": 89,
"end": 105
}
]
}
} | [] |
The glucocorticoid target tissues that have been examined (circulating mononuclear lymphocytes and cultured skin fibroblasts) have normal concentrations of glucocorticoid receptors with decreased affinity for dexamethasone. | {
"entities": {
"cell_line": [
{
"text": "cultured skin fibroblasts",
"start": 99,
"end": 124
}
]
}
} | [] |
Separate epitopes in VP22 were defined for T-cell clones from each of three patients. | {
"entities": {
"cell_line": [
{
"text": "T-cell clones",
"start": 43,
"end": 56
}
]
}
} | [] |
Some tegument-specific CD4 T-cell clones exhibited cytotoxic activity against HSV-infected cells. | {
"entities": {
"cell_line": [
{
"text": "tegument-specific CD4 T-cell clones",
"start": 5,
"end": 40
}
]
}
} | [] |
U937 cells differentiated with PMA in nonadherent culture were shown to express two fibrinogen-binding integrins, predominately CD11b/CD18, and to a lesser extent, CD11c/CD18. | {
"entities": {
"cell_line": [
{
"text": "U937 cells",
"start": 0,
"end": 10
},
{
"text": "nonadherent culture",
"start": 38,
"end": 57
}
]
}
} | [] |
To determine the effects on transcriptional regulation, U937 cells were transfected with a plasmid containing the HIV-1 enhancer (bearing two NF-kappa B sites) coupled to a chloramphenicol acetyltransferase (CAT) reporter. | {
"entities": {
"cell_line": [
{
"text": "U937 cells",
"start": 56,
"end": 66
}
]
}
} | [] |
Peripheral blood T cells and monocytes and B cell lines derived from patients with lupus express estrogen receptor transcripts similar to those of normal cells. | {
"entities": {
"cell_line": [
{
"text": "B cell lines",
"start": 43,
"end": 55
}
]
}
} | [] |
OBJECTIVE: To identify and characterize estrogen receptor (ER) transcripts expressed in immune cells of patients with systemic lupus erythematosus (SLE) and healthy donors. | {
"entities": {
"rna": [
{
"text": "estrogen receptor (ER) transcripts",
"start": 40,
"end": 74
}
]
}
} | [] |
Epstein-Barr virus-transformed B cell lines (n = 7) and B cell hybridomas (n = 2) established from patients with SLE and a healthy individual were used as a B cell source. | {
"entities": {
"cell_line": [
{
"text": "B cell lines",
"start": 31,
"end": 43
},
{
"text": "B cell hybridomas",
"start": 56,
"end": 73
}
]
}
} | [] |
These cells were examined for ER mRNA by reverse transcription nested polymerase chain reaction. | {
"entities": {
"rna": [
{
"text": "ER mRNA",
"start": 30,
"end": 37
}
]
}
} | [] |
RESULTS: In all cells tested, ER mRNA was expressed without prior in vitro stimulation. | {
"entities": {
"rna": [
{
"text": "ER mRNA",
"start": 30,
"end": 37
}
]
}
} | [] |
Partial sequences from exons 1-8 were nearly identical to the published sequence of the human ER mRNA. | {
"entities": {
"rna": [
{
"text": "human ER mRNA",
"start": 88,
"end": 101
}
]
}
} | [] |
There were no notable differences in the ER transcripts between patients and healthy controls. | {
"entities": {
"rna": [
{
"text": "ER transcripts",
"start": 41,
"end": 55
}
]
}
} | [] |
In vitro stimulation did not affect ER mRNA expression. | {
"entities": {
"rna": [
{
"text": "ER mRNA",
"start": 36,
"end": 43
}
]
}
} | [] |
Minimal residual disease in acute myelogenous leukemia with PML/RAR alpha or AML1/ETO mRNA and phenotypic analysis of possible T and natural killer cells in bone marrow. | {
"entities": {
"rna": [
{
"text": "AML1/ETO mRNA",
"start": 77,
"end": 90
}
]
}
} | [] |
Using the reverse transcription-polymerase chain reaction (RT) assay, the limit of detection was 10 (-5) to 10 (-6) for PML/RAR alpha transcript and 10 (-4) to 10 (-5) for the AML1/ETO transcript. | {
"entities": {
"rna": [
{
"text": "PML/RAR alpha transcript",
"start": 120,
"end": 144
},
{
"text": "AML1/ETO transcript",
"start": 176,
"end": 195
}
]
}
} | [] |
Using the 1G5 cell line harbouring the luciferase reporter gene under the control of the HIV-1 LTR, it was first found that culture protein filtrates (CFP) from M. tuberculosis or purified ManLAM could activate HIV-1 LTR-dependent gene expression unlike similarly prepared CFP extracts devoid of ManLAM. | {
"entities": {
"cell_line": [
{
"text": "1G5 cell line",
"start": 10,
"end": 23
}
]
}
} | [] |
Transient expression analyses in cells of neuroglial and lymphocytic origin demonstrated that some of these LTRs had activities which varied significantly from the LAI LTR in U-373 MG cells (an astrocytoma cell line) as well as in Jurkat cells (a CD4-positive lymphocyte cell line). | {
"entities": {
"cell_line": [
{
"text": "U-373 MG cells",
"start": 175,
"end": 189
},
{
"text": "astrocytoma cell line",
"start": 194,
"end": 215
},
{
"text": "Jurkat cells",
"start": 231,
"end": 243
},
... | [] |
While LTRs which demonstrated the highest activities in U-373 MG cells also yielded high activities in Jurkat cells, the LTRs were generally more active in Jurkat cells when compared to the LAI LTR. | {
"entities": {
"cell_line": [
{
"text": "U-373 MG cells",
"start": 56,
"end": 70
},
{
"text": "Jurkat cells",
"start": 103,
"end": 115
},
{
"text": "Jurkat cells",
"start": 156,
"end": 168
}
]
}
} | [] |
Transcriptional regulation of the gene for the myeloid calcium binding protein, MRP14, was investigated in human monocytic leukemia cell lines. | {
"entities": {
"cell_line": [
{
"text": "human monocytic leukemia cell lines",
"start": 107,
"end": 142
}
]
}
} | [] |
The MRP14 gene was not expressed in monoblastic ML-1 cells, promonocytic U-937 cells, or promyelocytic HL-60 cells. | {
"entities": {
"cell_line": [
{
"text": "monoblastic ML-1 cells",
"start": 36,
"end": 58
},
{
"text": "promonocytic U-937 cells",
"start": 60,
"end": 84
},
{
"text": "promyelocytic HL-60 cells",
"start": 89,
"en... | [] |
On the other hand, the gene was expressed in monocytic THP-1 cells and in the HL-60 cells treated with 1, 25-dihydroxyvitamin D3 (VD3). | {
"entities": {
"cell_line": [
{
"text": "monocytic THP-1 cells",
"start": 45,
"end": 66
},
{
"text": "HL-60 cells",
"start": 78,
"end": 89
}
]
}
} | [] |
The level of MRP14 in VD3-treated HL-60 cells was two-fold higher than that in THP-1 cells. | {
"entities": {
"cell_line": [
{
"text": "HL-60 cells",
"start": 34,
"end": 45
},
{
"text": "THP-1 cells",
"start": 79,
"end": 90
}
]
}
} | [] |
Among several known transcription factor binding motifs, nuclear protein (s) of VD3-treated HL-60 cells and THP-1 cells bound to the CCAAT/enhancer binding protein (C/EBP) -binding motif that was located in the upstream region of the MRP14 gene (-81), as evidenced by the competitive gel mobility-shift assay. | {
"entities": {
"cell_line": [
{
"text": "VD3-treated HL-60 cells and THP-1 cells",
"start": 80,
"end": 119
}
]
}
} | [] |
An antibody for C/EBP alpha super-shifted the nucleoprotein complex in THP-1 cells but not in the VD3-treated HL-60 cells, whereas an antibody for C/EBP beta blocked the formation of the complex with the nuclear factor of the HL-60 cells but not with that of THP-1 cells. | {
"entities": {
"cell_line": [
{
"text": "THP-1 cells",
"start": 71,
"end": 82
},
{
"text": "VD3-treated HL-60 cells",
"start": 98,
"end": 121
},
{
"text": "HL-60 cells",
"start": 226,
"end": 237
},
{... | [] |
Thus, it was concluded that C/EBP alpha and -beta were able to bind to the C/EBP motif, and that C/EBP alpha bound to the motif in THP-1 cells and C/EBP beta bound to that in the VD3-treated HL-60 cells. | {
"entities": {
"cell_line": [
{
"text": "THP-1 cells",
"start": 131,
"end": 142
},
{
"text": "VD3-treated HL-60 cells",
"start": 179,
"end": 202
}
]
}
} | [] |
Furthermore, to examine the transcriptional activity of the C/EBP motif, we transfected several constructed luciferase reporter DNAs into HL-60 cells and THP-1 cells. | {
"entities": {
"cell_line": [
{
"text": "HL-60 cells",
"start": 138,
"end": 149
},
{
"text": "THP-1 cells",
"start": 154,
"end": 165
}
]
}
} | [] |
The luciferase activity of the C/EBP motif in HL-60 cells was increased by VD3 treatment. | {
"entities": {
"cell_line": [
{
"text": "HL-60 cells",
"start": 46,
"end": 57
}
]
}
} | [] |
The C/EBP motif in the MRP14 gene was confirmed to function as a regulatory region in VD3-treated HL-60 cells and THP-1 cells by the assay. | {
"entities": {
"cell_line": [
{
"text": "VD3-treated HL-60 cells and THP-1 cells",
"start": 86,
"end": 125
}
]
}
} | [] |
Since C/EBP beta was also detected in VD3-untreated HL-60 cells by immunoblotting, VD3 activated C/EBP beta to bind to the motif, probably through post-translational modification. | {
"entities": {
"cell_line": [
{
"text": "VD3-untreated HL-60 cells",
"start": 38,
"end": 63
}
]
}
} | [] |
Steroid metabolism was investigated in cultured human B-lymphoblastoid cells (B-LCL), and peripheral blood T and B cells. | {
"entities": {
"cell_line": [
{
"text": "cultured human B-lymphoblastoid cells",
"start": 39,
"end": 76
},
{
"text": "B-LCL",
"start": 78,
"end": 83
}
]
}
} | [] |
Appropriate sized transcripts were detected in both cultured and fresh peripheral lymphocytes for CYP11A, CYP17, HSD11L (11beta-hydroxysteroid dehydrogenase I), HSD17B1 (17beta-hydroxysteroid dehydrogenase type I) and SRD5A1 (5alpha-reductase I). | {
"entities": {
"rna": [
{
"text": "transcripts",
"start": 18,
"end": 29
}
]
}
} | [] |
There was minimal expression of HSD3B1 and HSD3B2 (3beta-hydroxysteroid dehydrogenase I and II) in B-LCL and T cells. | {
"entities": {
"cell_line": [
{
"text": "B-LCL",
"start": 99,
"end": 104
}
]
}
} | [] |
Human white blood cells and hair follicles are good sources of mRNA for the pterin carbinolamine dehydratase/dimerization cofactor of HNF1 for mutation detection. | {
"entities": {
"rna": [
{
"text": "mRNA",
"start": 63,
"end": 67
}
]
}
} | [] |
Here we report for the first time that the PCD/DCoH mRNA is present in human white blood cells and hair follicles. | {
"entities": {
"rna": [
{
"text": "PCD/DCoH mRNA",
"start": 43,
"end": 56
}
]
}
} | [] |
In this report the biochemical properties of the heterotrimeric NF-Y complex have been characterized during stage-specific B-cell development, and in several class II- mutant B-cell lines, which represent distinct bare lymphocyte syndrome class II genetic complementation groups. | {
"entities": {
"cell_line": [
{
"text": "class II- mutant B-cell lines",
"start": 158,
"end": 187
}
]
}
} | [] |
The NF-Y complex derived from class II+ mature B-cells bound with high affinity to anion exchangers, and eluted as an intact trimeric complex, whereas, NF-Y derived from class II- plasma B-cells, and from bare lymphocyte syndrome group II cell lines, RJ2.2.5 and RM3, dissociated into discrete NF-YA and NF-YB: C subunit... | {
"entities": {
"cell_line": [
{
"text": "bare lymphocyte syndrome group II cell lines",
"start": 205,
"end": 249
},
{
"text": "RJ2.2.5",
"start": 251,
"end": 258
},
{
"text": "RM3",
"start": 263,
"end": 266
... | [] |
We found that the conversion of 70Z/3 pre-B lymphocytes to cells with a macrophage-like phenotype is associated with the loss of E2A and EBF. | {
"entities": {
"cell_line": [
{
"text": "70Z/3 pre-B lymphocytes",
"start": 32,
"end": 55
}
]
}
} | [] |
Moreover, we show that ectopic expression of the E2A protein E12 in this macrophage line results in the induction of many B lineage genes, including EBF, IL7Ralpha, lambda5, and Rag-1, and the ability to induce kappa light chain in response to mitogen. | {
"entities": {
"cell_line": [
{
"text": "macrophage line",
"start": 73,
"end": 88
}
]
}
} | [] |
Our data demonstrate that, in the context of this macrophage line, E12 induces expression of EBF and together these transcription factors coordinately regulate numerous B lineage-associated genes. | {
"entities": {
"cell_line": [
{
"text": "macrophage line",
"start": 50,
"end": 65
}
]
}
} | [] |
A 5.4-kilobase mRNA, the expression of which is down-regulated after treatment of human peripheral blood mononuclear cells (PBMCs) with various T cell-activating agents, was isolated using an mRNA differential display method. | {
"entities": {
"rna": [
{
"text": "5.4-kilobase mRNA",
"start": 2,
"end": 19
}
]
}
} | [] |
Nucleotide sequence analysis identified the 5' end of this RNA as human retinoid receptor RXRalpha mRNA. | {
"entities": {
"rna": [
{
"text": "5' end",
"start": 44,
"end": 50
},
{
"text": "RNA",
"start": 59,
"end": 62
},
{
"text": "human retinoid receptor RXRalpha mRNA",
"start": 66,
"end": 103
}
]
}
} | [] |
Here, we report the nucleotide sequence of 3.6 kilobases of this RNA, which represents the 3' end of RXRalpha mRNA, the sequence of which has not been previously described. | {
"entities": {
"rna": [
{
"text": "3' end",
"start": 91,
"end": 97
},
{
"text": "RXRalpha mRNA",
"start": 101,
"end": 114
}
]
}
} | [] |
Activated PBMCs also expressed lower levels of RXRalpha protein, and a DNA binding assay showed that the activation-induced loss of RXRalpha mRNA and protein expression correlated with the loss of DNA binding activity of this protein. | {
"entities": {
"rna": [
{
"text": "RXRalpha mRNA",
"start": 132,
"end": 145
}
]
}
} | [] |
The decrease in the levels of RXRalpha mRNA was found to be regulated at the post-transcriptional level and involved new protein synthesis. | {
"entities": {
"rna": [
{
"text": "RXRalpha mRNA",
"start": 30,
"end": 43
}
]
}
} | [] |
Use of transfected liver cells to evaluate potential mechanisms of alcohol-induced liver injury [see comments] | {
"entities": {
"cell_line": [
{
"text": "transfected liver cells",
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"end": 30
}
]
}
} | [] |
The objectives of these studies were to investigate mechanisms for induction of an injurious factor (IL-8) and a protective factor (MnSOD) in the HepG2 human hepatoma cell line. | {
"entities": {
"cell_line": [
{
"text": "HepG2 human hepatoma cell line",
"start": 146,
"end": 176
}
]
}
} | [] |
In the first set of experiments, IL-8 gene reporter constructs were used to transiently transfect a derivative (MVh2E1-9) of the HepG2 cell line which expresses P-4502E1 and metabolizes ethanol. | {
"entities": {
"cell_line": [
{
"text": "MVh2E1-9",
"start": 112,
"end": 120
},
{
"text": "HepG2 cell line",
"start": 129,
"end": 144
}
]
}
} | [] |
In the second set of experiments, HepG2 cells were cultured in 25 to 100 mmol concentrations of ethanol. | {
"entities": {
"cell_line": [
{
"text": "HepG2 cells",
"start": 34,
"end": 45
}
]
}
} | [] |
Both TNF and ethanol increased HepG2 cell MnSOD activity in short-term (72 hr) cultures with ethanol. | {
"entities": {
"cell_line": [
{
"text": "HepG2 cell",
"start": 31,
"end": 41
}
]
}
} | [] |
Further studies are needed to assess the effect of this diminished induction of MnSOD with chronic ethanol culture on HepG2 cell susceptibility to TNF cytotoxicity. | {
"entities": {
"cell_line": [
{
"text": "HepG2 cell",
"start": 118,
"end": 128
},
{
"text": "TNF cytotoxicity",
"start": 147,
"end": 163
}
]
}
} | [] |
We conclude that transfected liver cell lines can be used to evaluate mechanisms for increased injurious factors and decreased protective factors in alcoholic liver injury. | {
"entities": {
"cell_line": [
{
"text": "liver cell lines",
"start": 29,
"end": 45
}
]
}
} | [] |
MPs and HeLa cells subjected to hypoxia (pO2 approximately 13 torr) had increased levels of tissue factor transcripts (approximately 18-fold) and an increased rate of transcription (approximately 15-fold), based on nuclear run-on analysis. | {
"entities": {
"cell_line": [
{
"text": "HeLa cells",
"start": 8,
"end": 18
}
],
"rna": [
{
"text": "tissue factor transcripts",
"start": 92,
"end": 117
}
]
}
} | [] |
Gel-shift analysis of nuclear extracts from hypoxic MPs and HeLa cells demonstrated increased DNA-binding activity at the serum response region (SRR; -111/+14 bp) of the tissue factor promoter at Egr-1 motifs. | {
"entities": {
"cell_line": [
{
"text": "HeLa cells",
"start": 60,
"end": 70
}
]
}
} | [] |
Using 32P-labeled Egr consensus oligonucleotide, we observed induction of DNA-binding activity in nuclear extracts from hypoxic lung and HeLa cells because of activation of Egr-1, by means of supershift analysis. | {
"entities": {
"cell_line": [
{
"text": "HeLa cells",
"start": 137,
"end": 147
}
]
}
} | [] |
Transient transfection of HeLa cells with chimeric plasmids containing wild-type or mutant SRR from the tissue factor promoter showed that intact Sp1 sites are necessary for basal promoter activity, whereas the integrity of Egr-1 sites was required for hypoxia-enhanced expression. | {
"entities": {
"cell_line": [
{
"text": "HeLa cells",
"start": 26,
"end": 36
}
]
}
} | [] |
Kinetics of cytokine and NFAT gene expression in human interleukin-2-dependent T lymphoblasts stimulated via T-cell receptor. | {
"entities": {
"cell_line": [
{
"text": "human interleukin-2-dependent T lymphoblasts",
"start": 49,
"end": 93
}
]
}
} | [] |
Anti-CD3-restimulated T cells (mainly CD8+) produced IL-2, interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) and low levels of IL-4 and IL-10 transcripts and proteins. | {
"entities": {
"rna": [
{
"text": "IL-4 and IL-10 transcripts",
"start": 151,
"end": 177
}
]
}
} | [] |
The kinetics of TNF-alpha mRNA expression was faster, being at its peak level 1 hr after stimulation. | {
"entities": {
"rna": [
{
"text": "TNF-alpha mRNA",
"start": 16,
"end": 30
}
]
}
} | [] |
The transcriptional regulatory region of the gp34 gene was activated by HTLV-I Tax in the human T cell line Jurkat, in which endogenous gp34 is induced by Tax. | {
"entities": {
"cell_line": [
{
"text": "human T cell line Jurkat",
"start": 90,
"end": 114
}
]
}
} | [] |
Unlike typical NF-kappaB elements, the NF-kappaB-like elements in gp34 were not activated by treatment of Jurkat cells with phorbol ester despite induction of the NF-kappaB -like binding activity. | {
"entities": {
"cell_line": [
{
"text": "Jurkat cells",
"start": 106,
"end": 118
}
]
}
} | [] |
beta-Amyloid fibrils activate parallel mitogen-activated protein kinase pathways in microglia and THP1 monocytes. | {
"entities": {
"cell_line": [
{
"text": "THP1 monocytes",
"start": 98,
"end": 112
}
]
}
} | [] |
We report that exposure of primary rat microglia and human THP1 monocytes to fibrillar Abeta results in the tyrosine kinase -dependent activation of two parallel signal transduction cascades involving members of the mitogen-activated protein kinase (MAPK) superfamily. | {
"entities": {
"cell_line": [
{
"text": "human THP1 monocytes",
"start": 53,
"end": 73
}
]
}
} | [] |
Abeta stimulated the rapid, transient activation of extracellular signal-regulated kinase 1 (ERK1) and ERK2 in microglia and ERK2 in THP1 monocytes. | {
"entities": {
"cell_line": [
{
"text": "THP1 monocytes",
"start": 133,
"end": 147
}
]
}
} | [] |
Exposure of microglia and THP1 monocytes to Abeta resulted in the activation of RSK1 and RSK2 and phosphorylation of cAMP response element-binding protein at Ser133, providing a mechanism for Abeta -induced changes in gene expression | {
"entities": {
"cell_line": [
{
"text": "THP1 monocytes",
"start": 26,
"end": 40
}
]
}
} | [] |
The CRABP II mRNA was gradually upregulated during differentiation from MO to MAC in the presence of 2% serum. | {
"entities": {
"rna": [
{
"text": "CRABP II mRNA",
"start": 4,
"end": 17
}
]
}
} | [] |
In this study, we analyzed normal and malignant lymphoid tissues and cell lines, including 102 cases of B-cell NHL, 23 cases of T- and null-cell NHL, and 18 cases of Hodgkin's disease. | {
"entities": {
"cell_line": [
{
"text": "cell lines",
"start": 69,
"end": 79
}
]
}
} | [] |
Western blot analysis showed a 52-kD BSAP band in B-cell lines, but not in non-B-cell or plasma cell lines. | {
"entities": {
"cell_line": [
{
"text": "B-cell lines",
"start": 50,
"end": 62
},
{
"text": "non-B-cell or plasma cell lines",
"start": 75,
"end": 106
}
]
}
} | [] |
Isolation and analysis of a T cell clone variant exhibiting constitutively phosphorylated Ser133 cAMP response element-binding protein. | {
"entities": {
"cell_line": [
{
"text": "T cell clone variant",
"start": 28,
"end": 48
}
]
}
} | [] |
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