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0.474632
b91446ad98e94a13914d6c2cef5c96b7
a Analysis of TLGST on 12% SDS PAGE; (1) molecular weight marker proteins, (2) TLGST. b Analysis of TLGST on isoelectric focusing PAGE: (1) pI marker proteins, (2) TLGST
PMC9995618
43141_2023_486_Fig2_HTML.jpg
0.449689
64553ae7ca8448889e1804d8c8a6219a
a The pattern of TLGST pH profile utilizing 0.1 M potassium phosphate (5.7–8.0) and Tris–HCl (8.0–9.2). b Lineweaver–Burk plot of TLGST reaction speed in response to CDNB concentrations
PMC9995618
43141_2023_486_Fig3_HTML.jpg
0.409674
59cb93c9fd1743188ecde33c89cea0e2
a TLGST Inhibition with pCMB various concentrations. b Hill plot of TLGST inhibition with pCMB. c Lineweaver–Burk plots showing TLGST inhibition type with pCMB. d TLGST inhibition constant (Ki) value for pCMB
PMC9995618
43141_2023_486_Fig4_HTML.jpg
0.512687
03acf5abf14e40b599f720b4ca6f8b82
(A) JAK2V617F allele burden measured in the peripheral blood by digital droplet PCR during the study. (B) Cumulative change in hemoglobin (mmol/l) during the trial. (C) Cumulative change in leucocytes (×109/l) during the trial. (D) Cumulative change in platelets (×109/l) during the trial. (B, C) graphs depict the mean...
PMC9996128
fimmu-14-1117466-g001.jpg
0.41876
51c67ec280114147a21877c8261b70a2
Ex vivo IFNγ ELISPOT responses in patient PBMCs and BMNC during treatment. (A, B) Heat map of ex vivo PBMC responses against the ARG1 (A)- and PD-L1(B)-derived peptide epitopes at baseline and during treatment. (C): Representative well images of ex vivo IFNγ ELISPOT response against ArgLong2 and PD-L1Long1 in PBMCs fr...
PMC9996128
fimmu-14-1117466-g002.jpg
0.458695
064c61857f96406ba99a69aeacc0c9a9
Responses against ARG1- and PD-L1-derived peptides in PBMCs. (A, B): Heat maps depicting PBMC responses against the ARG1-(A) and PD-L1-(B) derived peptide epitopes as measured by in vitro IFNγ ELISPOT. The number of peptide-specific cells was calculated by subtracting the mean number of spots in the control wells from ...
PMC9996128
fimmu-14-1117466-g003.jpg
0.406722
6962e522165d4aa9b12469ca430c9302
Phenotypic marker expression in PBMCs and BMNCs at baseline and during vaccination trial. Percentage of CD3+ (A), CD4+ (B) and CD8+ (C) cells out of live cells in PBMCs of treated patients analyzed by flow cytometry. (D–G) changes in the subpopulations of CD8+ T cells in the PBMCs of treated patients. (D) Fraction of C...
PMC9996128
fimmu-14-1117466-g004.jpg
0.495684
889918128e794bdf90d0a068cab0af58
Surveyed sites in Yunnan Province, southwestern China. (A) Tropical region (Xishuangbanna Banma Mountain: 800, 1,000, 1,200, and 1,400 m); (B) Subtropical region (Puer Ailao Mountain: 2,000, 2,200, 2,400, and 2,600 m); (C) Subalpine region (Lijiang Yulong Snow Mountain: 3200, 3,400, 3,600, and 3,800 m). The red dots in...
PMC9996296
fmicb-14-1079113-g001.jpg
0.522513
49ee62aaa0334fba8e2cbd3dc8e559d9
Tree, fungal, and bacterial species along elevational gradients in three climatic zones. (A): tropical; (B): subtropical; (C): subalpine; A (1–3), B (1–3), C (1–3): tree species; A (4–6), B (4–6), C (4–6): fungal species; A (7–9), B (7–9), C (7–9): bacterial species. SR: species richness; PD: phylogenetic diversity; Sh...
PMC9996296
fmicb-14-1079113-g002.jpg
0.388
fc75070ad52942328a812ab0b2163682
Analysis of environmental factors and diversity indexes in three climatic zones using random forest. A-C: tree species; D-F: fungal species; G-I: bacterial species; OM: soil organic matter content; TC: total carbon; TN: total nitrogen; HN: hydrolysable nitrogen; TP: total phosphorus; TK: total potassium; AK: available ...
PMC9996296
fmicb-14-1079113-g003.jpg
0.411499
5e5bba30d5cb489bb4697cbcba50618e
The explanatory power of soil factors for species composition in three climatic zones. A-C: tree species; D-F: fungal species; G-I: bacterial species. OM: organic matter; TC: total carbon; TN: total nitrogen; HN: hydrolysable nitrogen; TP: total phosphorus; TK: total potassium; AK: available potassium; pH: soil pH; Wat...
PMC9996296
fmicb-14-1079113-g004.jpg
0.448577
d3ca3307a75e461aae2bfdf8f4f548a7
Piecewise structural equation model. We performed principal component analysis of environmental factors (climatic and soil properties), plant diversity, and microbial diversity to extract data on the first axis (PC1, explained variance >70%). Model A: environmental factors directly affect microorganisms and plants. Mod...
PMC9996296
fmicb-14-1079113-g005.jpg
0.450263
4f56ca6174994229bb78caed453a531f
Chemically modified aptamers for improving binding affinity to the target protein via enhancing non-covalent bonding (the red dot represented amino acid residues on the protein, the yellow rectangle represented modified nucleotides on the aptamer, the black arrow represented non-covalent bond).
PMC9996316
fcell-11-1091809-g001.jpg
0.452795
0aa98899a7f64fda9b519258fcd0abf9
The nucleobases modifications on the aptamers to enhance binding affinity. (A) SOMAmers modification; (B) Modified uridine by click chemistry; (C) Base-appended bases modification; (D) Amino acid (leu) modification.
PMC9996316
fcell-11-1091809-g002.jpg
0.598629
bb3e0d208b314135b52a517d0c2eb724
The ribose modifications on the aptamers to enhance binding affinity. (A) 2′-fluoro (2′-F) modification; (B) 2′-Fluoro Arabino nucleic acid modification; (C) 2′, 2′-difluoro-2′-deoxycytidine modification; (D) Locked Nucleic Acid modification; (E) Unlocked Nucleic Acid modification.
PMC9996316
fcell-11-1091809-g003.jpg
0.50614
ab55fc22d08d4115a0f3fb1cf5fd9b17
The ribose modifications on the aptamers to enhance binding affinity. (A) 4′-S modification; (B) TNA modification; (C) HNA modification; (D) phNA modification.
PMC9996316
fcell-11-1091809-g004.jpg
0.561396
0a4bd3f65dc64a458cae7a39b9b5fd8d
The mirror-image nucleotide (L-DNA) modification on aptamers.
PMC9996316
fcell-11-1091809-g005.jpg
0.53089
4498fc7c8cf246ec8f08a7cf6f6a671a
The chemical modifications on the phosphate of aptamers to improve affinity. (A) Phosphorothioate (PS) modification; (B) phosphorodithioate (PS2) modification.
PMC9996316
fcell-11-1091809-g006.jpg
0.532913
2578ce4f6dc646da8141f71d3c2b3aca
The artificial nucleotides incorporating into aptamer to improve binding affinity. (A) The (7- (2-thienyl) imidazo [4, 5-b] pyridine) (Ds) artificial nucleotide; (B) the 6-amino-5-nitro-3- (1′-β-D-2′-deoxyribofuranosyl)-2 (1H)-pyridone (Z) artificial nucleotide; (C) the 2-amino-8- (1′-β-D-2′-deoxy-ribofuranosyl)-imidaz...
PMC9996316
fcell-11-1091809-g007.jpg
0.428593
a32a97ecc19a4b89868b1b53e59df19c
(A) Experimental workflow for the labeling of co-translational O-GlcNAcylation and boosting samples. In the boosting sample, O-GlcNAcylated proteins were labeled with Ac4GalNAz for 48 h in heavy media. For the co-translational and control samples, the culture media were switched from light to heavy, and cells were furt...
PMC9996615
ac2c04779_0002.jpg
0.428092
dcf3bac689fb4bcb8fc12e0965079a3c
(A) Example tandem mass spectrum of an identified co-translational O-GlcNAcylated peptide. (B) Correlations of the quantified O-GlcNAcylated peptides with the Puro treatment in the duplicate experiments. (C) Correlations of the quantified O-GlcNAcylated peptides in the duplicate control experiments. (D) Ratios of the i...
PMC9996615
ac2c04779_0003.jpg
0.450953
6200ece5ac2e4f7290b53ffe7bd9e3b8
(A) Clustering results for all O-GlcNAcylated proteins against the human proteome. (B) Clustering results for co-translational O-GlcNAcylated proteins against total O-GlcNAcylated proteins identified in MCF7 cells. (C) Comparison of the distribution of the proteins with co-translational glycosylation sites and others i...
PMC9996615
ac2c04779_0004.jpg
0.43191
f65e4f89d76d49399ad9c088832af535
(A and B) Illustrations of the chromatin remodeling complex (A) and the spliceosome (B). (C) Identification of co-translational O-GlcNAcylated proteins associated with different protein complexes and biological processes. Each node stands for a protein, and each edge represents a known protein–protein interaction. The ...
PMC9996615
ac2c04779_0005.jpg
0.447743
726628089f2444458fc5eab49408ca03
(A–C) Comparison of the amino acid composition (A), the GRAVY score (B), and isoelectric point (C) of the adjacent residues between the co-translational and total O-GlcNAcylation sites. (D) Occurrence of different amino acids next to the co-translational O-GlcNAcylation sites, using all O-GlcNAcylation sites identified...
PMC9996615
ac2c04779_0006.jpg
0.382567
3e661aa9b0e54ef2a528926e830af8ba
CNVs overlapping between SPACNACS and other databases. Comparative between the CNVs found in SPACNACS and the ones present in the 1000 genomes and Gnomad databases. The X axis incrementally represents the level of overlap between the CNVs compared, which range from 0 (CNVs unique to SPACNACS) up to 100% (CNVS with a pe...
PMC9997023
40246_2023_466_Fig1_HTML.jpg
0.478045
1b200452f1974b3684dea4304775221a
The SPACNACS interface. A Genome browser panel consisting of an embedded Integrative Genomics Viewer preloaded with the Spanish CNV database and other useful tracks. B Search and selection panel, which provides several filters for specifying the genomic region and the data to be shown. C Filtering status panel, which s...
PMC9997023
40246_2023_466_Fig2_HTML.jpg
0.446868
aee31d6ee55d47d1bf8d280232ccef0f
Distribution of CNVs detected in pharmacogenes (PGx genes) according to the allele frequency. The number of genes harboring duplications (green) or deletions (red) of the 1045 PGx genes tested are shown according to the frequency detected in the population
PMC9997023
40246_2023_466_Fig3_HTML.jpg
0.44375
183d342ed0054377b0eda0e87d1f3ae2
Daily intake of mineral (MIN) or energy with mineral (NRG) supplements of heifers grazing native range over the duration of the grazing season. The 57-d monitoring period was initiated from the time of pregnancy diagnosis (July 25th) until removal from pasture (September 19th). Treatments include: MIN (N = 18), free ch...
PMC9997776
txad013_fig1.jpg
0.460935
01ca4f7a64744561bdee7f983a7e0ecf
Effects of VDR deletion on LPS-induced AKI mice. (A) Serum concentrations of BUN and Cr at 24 h after LPS administration. (B) H&E staining of kidney sections. (C) Immunofluorescence analysis and its quantitative analysis of TUNEL (top) and F4/80 (bottom) of kidney sections. (D) Western blot analysis (left) and densitom...
PMC9998528
fphys-14-1083643-g001.jpg
0.435933
ec6f74b1411345febb9fe0dff92b3850
VDR deletion aggravated the abnormal glycolysis of LPS-induced AKI mice. (A) Renal lactate content and hexokinase activity of the four groups. (B) Western blot analysis (left) and densitometric quantitation (right) of PDHA1 and p-PDHA1 and was performed in the four groups of mice. (C) Immunofluorescence analysis and i...
PMC9998528
fphys-14-1083643-g002.jpg
0.374142
d13a9c0179e24bad97771e4cab741f29
Effects of VDR overexpression on LPS-induced AKI mice. (A) Serum concentrations of BUN and Cr at 24 h after LPS administration. (B) H&E staining of kidney sections. (C) Immunofluorescence analysis and its quantitative analysis of TUNEL (top) and F4/80 (bottom) of kidney sections. (D) Western blot analysis (left) and de...
PMC9998528
fphys-14-1083643-g003.jpg
0.44532
ac0e5d67d4bb4ac9b2038a138043968d
VDR overexpression lightened the abnormal glycolysis of LPS-induced AKI mice. (A) Renal lactate content and hexokinase activity of the four groups. (B) Western blot analysis (left) and densitometric quantitation (right) of PDHA1 and p-PDHA1 and was performed in the four groups of mice. (C) Immunofluorescence analysis ...
PMC9998528
fphys-14-1083643-g004.jpg
0.423126
d22bcf12b33f4e939b4e7263aadfb13c
Paricalcitol alleviated renal injury on LPS-induced AKI mice. (A) Serum concentrations of BUN and Cr at 24 h after LPS administration. (B) H&E staining of kidney sections. (C) Immunofluorescence analysis and its quantitative analysis of TUNEL (top) and F4/80 (bottom) of kidney sections. (D) Western blot analysis (left)...
PMC9998528
fphys-14-1083643-g005.jpg
0.41249
4ed5b8feaf294f24afb819e2ff360816
Paricalcitol alleviated glucose metabolism reprogramming of LPS-induced AKI mice. (A) Renal lactate content and hexokinase activity of the four groups. (B) Western blot analysis (left) and densitometric quantitation (right) of PDHA1 and p-PDHA1 and was performed in the four groups of mice. (C) Immunofluorescence analys...
PMC9998528
fphys-14-1083643-g006.jpg
0.494865
b06f3e94c58d4743b2fcaeebf5655699
VDR deletion aggravated abnormal glycolysis and injury in LPS-induced HK-2 cell. (A) Oxygen consumption rate (OCR) (top) measured by Seahorse metabolic analyzer and quantitative analysis (bottom) of mitochondrial function parameters (basal respiration, maximal respiration). (B) lactate content in HK-2 cells and VDR-KO...
PMC9998528
fphys-14-1083643-g007.jpg
0.487055
d0ddbdd518464739982dc87e51188b40
VDR overexpression lightened LPS-induced abnormal glycolysis and injury in HK-2 cell. (A) Oxygen consumption rate (OCR) (top) measured by Seahorse metabolic analyzer and quantitative analysis (bottom) of mitochondrial function parameters (basal respiration, maximal respiration). (B) lactate content in HK-2 cells and V...
PMC9998528
fphys-14-1083643-g008.jpg
0.436667
c0bcb48ca96444c0891fb8df339fe31d
Paricalcitol alleviated LPS-induced injury through phosphorylation of PDHA1. (A) Oxygen consumption rate (OCR) (top) measured by Seahorse metabolic analyzer and quantitative analysis (bottom) of mitochondrial function parameters (basal respiration, maximal respiration). (B) lactate content in HK-2 cells treated with LP...
PMC9998528
fphys-14-1083643-g009.jpg
0.459719
f30f107b06dd4cf7b18d46af9843b14f
VD-VDR active AMPK in LPS-induced AKI mice. (A) Western blot analysis (left) and densitometric quantitation (right) of AMPK and p-AMPK was performed in group of WT, KO, LPS and KO + LPS. (B) Western blot analysis (left) and densitometric quantitation (right) of AMPK and p-AMPK was performed in group of WT, OE, LPS and ...
PMC9998528
fphys-14-1083643-g010.jpg
0.423855
8e65b8dbeafb48a58416982839d473d5
VD alleviate glucose metabolism reprogramming via the activation of AMPK pathway. (A) Western blot analysis (left) and densitometric quantitation (right) of AMPK and p-AMPK in VDR-KO cells treated with LPS for 24 h. (B) Western blot analysis (left) and densitometric quantitation (right) of AMPK and p-AMPK in VDR-OE cel...
PMC9998528
fphys-14-1083643-g011.jpg
0.435925
3194a16cc14c49759acddbf22e437a9c
Imaging findings. (A) Axial non-contrast T1-weighted breast MRI image shows no evidence of malignancy. There is a mediastinal shift with the heart shifted to the right and a lack of lung markings in the upper left lung, suggesting the possibility of bulla in the upper left lung. (B) Chest X-ray shows a left-sided pneum...
PMC9999020
fonc-13-1096160-g001.jpg
0.456443
6e2a56c0709547aa8ede97bf82f2caf3
Pathological features. The tumor is represented by cystic and solid components (A); HE original magnification x5. The solid component is composed of spindle cells forming fascicles (B); there is variable background collagen deposition; HE original magnification x20. The tumor cells are immunoreactive with bcl2 (C) and ...
PMC9999020
fonc-13-1096160-g002.jpg