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61,917 | [The "free" erythrocytes in newborn infants]. | The percentage of non-A and non-AHP erythrocytes in newborns is usually increased and presents individual differences. It does not depend on the A1/A2 subgroups. These individual differences also concern the relationship between the number of non-A erythrocytes and of non-AH P-RBC. After delivery the A and AHP agglutin... |
61,918 | [The demonstration of HL-A antigens on thrombocytes under various conditions]. | Platelets of healthy test persons were gained according to the method of Aster (avital cells) and according to the procedure of BUBE and GMURZYNSKI (vital cells). Because of their tendency towards aggregation vital cells do bind any HL-A antibodies and whereas avital cells will fix antibodies and complement depending o... |
61,919 | The influence of azathioprine on the behaviour of blood platelets in vitro. | The influence of azathioprine on human platelets was studied in vitro. Azathioprine in a final concentration of 10(-4) M caused significant inhibition of spontaneous sedimentation and aggregation of platelets, but did not effect platelet adhesiveness or resistance to freezing and thawing. The influence of azathioprine ... |
61,920 | [The reactivity of the anticoagulatory system in sympathectomized animals under stress conditions due to acoustic trauma]. | In rats of kind of Kruschinskij-Molodkina the number of cells in the stellate ganglion was diminished to 0.5% of the norm after eliminating the sympathetic tonus by means of guanethidine. These animals died of cardiac thrombosis during stress situations. This thrombosis cannot be explained by adrenalin, corticosteroids... |
61,921 | Failure of tranexamic acid to influence the ellagic acid-induced hypercoagulable state. | Tranexamic acid in a dose of 50 mg/kg b.w. was unable to alter the ellagic acid induced hypercoagulable state. No change in the hypercoagulability pattern was observed regardless of the time of administration of the compound (before or after the ellagic infusion). The silicone clotting times after the infusion of ellag... |
61,922 | Relations between fibrinogen degradation products and heparinocytes. | In the emergency reaction there is a short-time increase of the values of basophilic leukocytes in connection with normal values of fibrinogen degradation products (FDP). After diminuation of basophils FDP are to be detected. In the chronical ill and under different hormonal contraceptives these two processes are overl... |
61,923 | Suppression of photo-induced sporulation in Trichoderma viride by inhibitors. | The mycelium of Trichoderma viride grown in the dark under submerged conditions and transferred to membrane filters sporulated only after photoinduction. The optimum photoinduction of sporulation was reached when applying daylight for 3 min and near ultraviolet radiation (355 nm) for 10 to 30 sec. After the photoinduct... |
61,926 | Determination of electrolytes in the myocardium as a tool for the post-mortal diagnosis of recent infarction. | The K/Na quotient in the myocardium has been compared to the result of the PTAH staining method in an autopsy material of verified myocardial infarctions, suspected but macroscopically unverified infarctions and controls. The control cases regularly had quotion showed ischemic changes in PTAH staining. These and additi... |
61,934 | [The effect of bencyclane hydrogen fumarate (Fludilate) on the adhesion of tumor cells in vivo and in vitro]. | Bencyclane hydrogen fumarate (Fludilat) was tested on the stickiness of tumor cells in vivo and in vitro. It was intended to determine whether Fludilat reduced the cancer cell stickiness in vitro, and if the survival time of cancer cell carrying animals can be increased with Fludilat in vivo, or in combination with a c... |
61,937 | Effect of bursa Fabricius extracts on antibody production in bursectomized or bursal cell autografted chickens. | Restoration and enhancement of immune response against BSA antigen was achieved by 5-day consecutive doses of BF estract from 4-5-week-old chickens, in birds which had been surgically bursectomized or given BF-cell autografts at 17 days of age. A similar 5-day treatment with other tissue extract, i.e. liver, spleen, pa... |
61,930 | Practical approach to the diagnosis of sudden unexpected death of cardiac origin. | Experiences concerning the practical demonstration of recent myocardial lesion (infarction) with various conventional and enzyme-histochemical methods are explained. It has been found in our laboratory that besides careful inspection of the heart, additional useful information can be obtained with ordinary H-E staining... |
61,929 | Experiences with the hematoxylin basic fuchsin picric acid staining method for morphologic diagnosis of myocardial ischemia - an experimental study in forensic pathology. | An investigation was performed on 148 medicolegal autopsy cases with the purpose of obtaining experience with the hematoxylin basic fuchsin acid staining method for morphologic diagnosis of early myocardial ischemia. A comparative study was performed on rats with induced myocardial infarcts. The uptake of the basic fuc... |
61,939 | Rat parietal yolk sac basement membrane. An investigation of the antigenic determinants using a radioimmunoassay. | Previous studies have shown that there is microscopic and biochemical evidence that rat parietal yolk sac synthesizes basement membrane (type IV) collagen; this study shows that a radioimmunoassay may be used for the detection of type IV collagen in such biosynthetic systems. Rat parietal yolk sacs incubated in medium ... |
61,938 | Long-term antibody synthesis in vitro- IV. Independent segregation of antibodies directed to different determinants of an antigen molecule in its native configuration. | Independent segregation of antibody populations directed to different portions of E. coli beta-d-galactosidase occurs during the immune response against the enzyme. Anti-enzyme antibodies able to interact and activate a naturally occurring ligand, the mutant-defective enzyme AMEF (Antibody Mediated Enzyme Factor), do n... |
61,940 | Regulatory effect of temperature and antigen upon immunity in ectothermic vertebrates. I. Influence of hapten density on the immunological and serological properties of penicilloyl-carrier conjugates. | Sodium penicillin was conjugated to sheep erythrocytes and optimal quantities, added to a 5% SRBC suspension, were determined for haemagglutination (12-5 mg/ml) and for haemolysis (50 mg/ml) using carp antibodies and carp complement. The epitope density on the BSA molecule was gradually increased, when increasing amoun... |
61,943 | Distribution of ad and ay subtypes of hepatitis B surface antigen among hepatitis patients and symptomless carriers in Hungary. | A total of 176 HBsAg positive sera from acute and chronic hepatitis patients and symptomless carriers (blood donors) in Hungary were subtyped for ad and ay determinants. The distribution of ad and ay determinants in Hungary was found to be about the same. There were no significant differences among the groups tested. |
61,944 | Specificity of serum from rodents immune to Moloney C-type virus-induced tumours. | When cells are infected by C-type viruses such as Moloney sarcoma/leukaemia virus, new antigens appear on the cell membrane. Mice and rats will respond immunologically to the antigen(s). It was uncertain whether the antigens were related to the viral structural proteins or to non-virion, tumour-specific surface antigen... |
61,945 | Studies on Epstein-Barr virus-related antigens. I. Indirect single radial immunodiffusion as a useful method for detection and assay of soluble antigen. | A useful method for the detection and assay of Epstein-Barr virus (EBV)-related soluble antigen has been developed by the application of the indirect single radial immunodiffusion technique which is frequently used for quantitative measurements of immunoglobulins and other soluble proteins. When the extracts of EBV-det... |
61,946 | In vitro stimulation of mouse lymphoid cells by C1300 neuroblastoma cells or tumor membrane extracts. | Spleen lymphoid cells from A/J mice recognize specific antigenic differences on the surface membranes of syngeneic C1300 neuroblastoma cells and incorporate 3H-thymidine into DNA in unidirectional mixed cell cultures in the absence of isologous serum. The response requires an optimal ratio of responder to stimulator ce... |
61,947 | Interaction of bleomycin and radiation in combined treatment on mouse L cells. | Using an established line of L cells, we studied survival properties and recovery after combined action of bleomycin and radiation. A moderate synergistic effect was observed when bleomycin and radiation were given simultaneously. Cell recovery from radiation damage was not affected by bleomycin. Results seem to be in ... |
61,951 | A new pyrazolidine derivative - benetazone spofa - in short- and medium-term treatment of rheumatoid arthritis. (Double-blind comparative study with phenylbutazone). | The effects of Benetazone Spofa (trimethazone) in a dose of 1000 mg/day with those of phenylbutazone in a dose of 600 mg day in patients with rheumatoid arthritis were tested in a short-term double-blind trial (3 weeks) and in a long-term double-blind trial (12 weeks). The short-term trial failed to disclose a signific... |
61,952 | Double-blind controlled trial of the new preparation FZ. 560 in the symptomatic treatment of digestive disorders. | A controlled double-blind clinical trial was carried out to compare the effects of a new combination FZ. 560 in the symptomatic treatment of digestive disorders including a wide variety of symptoms. The combination contained fentonium bromide, 10 mg, dehydrocholic acid, 25 mg, pancreatin 3FU, 50 mg, and lactulose, 200 ... |
61,950 | The use of non-deparaffinized tissue sections for staining leprosy bacilli. | Reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. By the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly staine... |
61,949 | Immunologic identification of M. leprae. Immunofluorescence and complement fixation. | A markedly improved immunofluorescent technic employing FITC conjugated IgG antibody prepared from lepromatous serum is described as a means of specific identification of M. leprae. An additional immunologic identification method for M. leprae is presented as a micro-complement fixation technic employing antigen rather... |
61,962 | [Histologic data on the lingual glands in various anuran amphibians]. | The anuran Amphibians have a well developed tongue which is either protractile or attached to the floor of the buccal cavity according to the species. The lingual glands of the papillary dorsal side consist of simple tubules. The stratified epithelium of the ventral and lateral sides of the tongue is composed of mucous... |
61,961 | Evidence for a role of N-acetylmuramyl-L-alanine amidase in septum separation in Escherichia coli. | Septum formation and septum separation have been studied in a chain-forming mutant of Escherichia coli K-12 bearing the envA mutation and its parental strain. In comparison to the wild type, the mutant showed a sixfold reduction in the specific activity of the enzyme, N-acetylmuramyl-L-alanine amidase (EC 3.5.1.28), pa... |
61,963 | Inhibition of activated factor XII by antithrombin-heparin cofactor. | The activation of Factor XII occurs via fragmentation of this zymogen into a diverse spectrum of enzymatically potent molecular species. To study the interaction of antithrombin-heparin cofactor and heparin with activated Factor XII, we have employed two forms of this enzyme with widely differing physical characteristi... |
61,964 | Binding of thyroid hormones and their analogues to thyroxine-binding globulin in human serum. | The present study was undertaken to study the binding of several thyroid hormones and structurally related compounds to human serum thyroxine-binding alpha-globulin (TBG). The source of TBG was normal human serum diluted 1:100 in 0.035 M barbital buffer, pH 7.4. In the binding assays, 125I-thyroxine, unlabeled thyroxin... |
61,965 | Studies on the structural localization of rabbit H chain allotypic determinants controlled by the a locus. Purification and immunological properties of an immunopeptide bearing a3 allotypic determinants. | An immunopeptide bearing a3 allotypic determinant(s) was isolated from the gamma chain of an a3 homozygous rabbit (G222-2) immunized with type III pneumococcal vaccine. Immunocogical properties of peptides were studied using a radioimmunoassay that involved inhibition by these peptides of a reaction between 125I-labele... |
61,966 | Flow cytofluorometric analysis of cell cycle distributions using propidium iodide. Properties of the method and mathematical analysis of the data. | In order to better characterize the new rapid staining method for flow cytofluorometry proposed by Krishan, we have tested its stability and several other properties, and have carried out a quantitative comparison of the fluorescence histograms obtained using propidium iodide or the acriflavine-Feulgen staining procedu... |
61,967 | Effect of colchicine on rat mast cells. | In the mast cell, a well-developed array of microtubules is centered around the centrioles. Complete loss of microtubules is observed when mast cells are treated with 10(-5) M colchicine for 4 h at 37 degrees C. The loss of ultrastructurally evident microtubules is associated with a marked change in the shape of mast c... |
61,968 | Apparent anomalies in nuclear feulgen-DNA contents. Role of systematic microdensitometric errors. | The Feulgen-DNA contents of human leukocytes, sperm, and oral squames were investigated by scanning and integrating microdensitometry, both with and without correction for residual distribution error and glare. Maximally stained sperm had absorbances which at lambdamax exceeded the measuring range of the Vickers M86 m... |
61,969 | Immunofluorescence evidence for the absence of histone H1 in a mitotically dividing, genetically inactive nucleus. | Antibodies directed against whole histone and purified lysine-rich histone H1 extracted from isolated macronuclei of the ciliate Tetrahymena were obtained and conjugated to fluorescein isothiocyanate. The fluorescein-antibody conjugates were used to directly label Tetrahymena cells. Both macro- and micronuclei were vis... |
61,970 | Preclinical hyperthyroidism in multinodular goiter. | The thyrotropin (TSH) response to thyrotropin-releasing hormone(TRH) (200 mug iv) was determined in 80 surgical patients with nontoxic multinodular goiter. The TSH reserve was normal in multinodular goiter. The TSH reserve was normal in 55 and elavated in 8 patients. No TSH response to TRH (deltaTSH less than or equal ... |
61,971 | A study of possible biohazards in the fluorescent antibody test using adenovirus, coxsackievirus, herpesvirus, and respiratory syncytial virus as antigens. | Infectious adenovirus type 5 and coxsackievirus type B5, both nonlipid-containing viruses, were isolated from cells fixed in acetone at 22 degrees C for 15 min, from acetone used for fixation, from the solution used for washing slides during the fluorescent antibody procedure, and after complete processing of antigen p... |
61,972 | Microscopy of stained urine smears to determine the need for quantitative culture. | Consecutive specimens (2,564) of urine were cultured quantitatively, and Gram-stained smears were prepared from centrifugates as well as from the uncentrifuged specimens. About half of the specimens harbored organisms, and the quantity seen in smears of the centrifugates correlated reasonably well with the numbers of v... |
61,973 | Comparison of machine and manual staining of direct smears for acid-fast bacilli by fluorescence microscopy. | Comparisons were made in Lusaka and in London between manual staining and staining in an automatic machine with auramine-phenol of direct smears of sputum and other types of specimen for acid-fast bacilli. No evidence was obtained of carry-over of acid-fast bacilli from positive to negative smears during machine staini... |
61,974 | Changes in protease inhibitors after acute myocardial infarction. | Plasma levels of fibrinogen, alpha1-antitrypsin, alpha2-macroglobulin, antithrombin III, and C1 inactivator were measured serially for 10 days in 11 patients after acute myocardial infarction. Both fibrinogen and alpha1-antitrypsin rose markedly to reach peak levels 5-7 days after infarction while C1 inactivator levels... |
61,975 | The efferent connections of the ventromedial nucleus of the hypothalamus of the rat. | The efferent connections of the ventromedial nucleus of the hypothalamus (VMH) of the rat have been examined using the autoradiographic method. Following injections of small amounts (0.4-2.0 muCi) of tritium labeled amino acids, fibers from the VMH can be traced forward through the periventricular region, the medial hy... |
61,976 | Origins of axons in the cat's acoustic striae determined by injection of horseradish peroxidase into severed tracts. | Origins and terminations of fibers of the dorsal and intermediate acoustic striae were studied by surgically severing these tracts and injecting HRP into the incision. This procedure results in filling the severed axons with HRP. Filled axons were traced to cell groups of origin and to some terminations of the acoustic... |
61,977 | The effect of atropine and albuterol aerosols on the human bronchial response to histamine. | This study was designed to determine whether histamine-induced bronchoconstriction in human asthmatics is mediated by the parasympathetic nervous system and involves cholinergic pathways. Inhalation challenges were performed on 14 adult asthmatic patients using the standardized procedure for inhalation challenge recent... |
61,979 | Rat urinary metabolites from O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl) phosphorothioate. | Rats metabolized single oral doses of O,O-diethyl-O(3,5,6-trichloro-2-pyridyl-2,6-14C) phosphorothioate to at least six radiolabeled urinary metabolites. The urine contained about 90 percent of the dose. Three of these metabolites were identified as the glucuronide of 3,5,6-trichloro-2-pyridinol (80% the urinary 14C), ... |
61,980 | 1,2-Benzanthracene in soil. | Samples from three cultivated soils and one from a roadside, all in Ontario, were found to contain less than 1 to 68 ppb of 1,2-Benzanthracene (BA). Two plots subjected to stubble (residue of wheat crop) burning annually for 15 years did not contain significant amounts of BA, although polyaromatic hydrocarbons, includi... |
61,989 | Immunocytochemical identification and localization of immunoglobulin A within Paneth cells of the rat small intestine. | Light microscopic immunocytochemistry was used to identify Paneth cells by their lysozyme content and to detect immunoglobulin antigens within a subpopulation of these cells. Antisera specific for the heavy chains of rat or human immunoglobulin A and for immunoglobulin light chain antigens produced specific staining of... |
61,990 | Virus-enhanced modulation of cell surface antigens: effect on immune lytic susceptibility. | Monkey kidney cells, upon progressive subculture, became refractory to complement (C)-dependent immune cytolysis by anti-cell serum. Arbovirus infection restored these cells to a state of lytic susceptibility. Similar results were also abtained with antibody-dependent cellular cytotoxicity (ADCC), which is C independen... |
61,991 | Coexistence of helper and suppressor activities in carrier-primed spleen cells. | Both helper and suppressor activities for an in vitro IgG anti-hapten (Lac) response to Lac-HRBC were demonstrated in the same population of carrier (HRBC)-primed spleen cells. The relative radiosensitivity of suppressor activity permitted the selective removal of suppression and the demonstration of help. Both activit... |
61,992 | Specific, transient suppression of the immune response by HGG tolerant spleen cells. II. Effector cells and target cells. | In a previous report, it was shown that spleen cells from mice made tolerant to human gamma-globulin (HGG)5 could specifically inhibit the immune response of normal spleen cells after adoptive transfer to lethally irradiated recipients. However, that report also showed that the suppressive activity was only transiently... |
61,993 | Distinct target determinants on two lymphoblastoid lines derived from the same individual1. | Specifically cytotoxic cells were generated in undirectional mixed leukocyte culture against both T and B lymphoblastoid cell lines. The cytotoxicity observed included both specific and less selective components which were differentiated by the interaction analysis based on the two-way analysis of variance. The results... |
61,981 | [Glycoproteins in maternal and fetal blood and amniotic fluid. Variations in normal and pathologic pregnancies]. | In this brief note, which is a preliminary report, the authors investigated systematically the variations, during normal and complicated pregnancies, of the concentrations of alpha-1-antitrypsin, orosomucoid, transferrin and alpha feto-protein, simultaneously in maternal serum, fetal serum and amniotic fluid. There exi... |
61,994 | Reaginic antibody formation in the mouse. VII. Depression of the ongoing IgE antibody formation by suppressor T cells. | The ongoing IgE antibody formation against ovalbumin (OA) in high responder mice was depressed by i.v. injections of either native or urea-denatured ovalbumin (UD-OA). Adoptive transfer experiments to determine the helper function of spleen cells from the treated animals showed that helper function for both IgE and IgG... |
61,995 | Macrophage-lymphocyte interaction. III. Site of alloantiserum inhibition of T lymphocyte proliferation induced by allogeneic or aldehyde-bearing cells. | Inhibition by anti-Ia sera of guinea pig T lymphocyte proliferation induced by allogeneic macrophages (MLR) and NaIO4 or neuraminidase-galactose oxidase-treated macrophages has been investigated in order to identify the target cell upon which the antisera act. Anti-2 and anti-13 alloantisera were found to inhibit both ... |
61,982 | [Alpha feto protein. Normal pregnancies and maternal diseases. Apropos of 3,010 radioimmunologic determinations]. | Using an immunological technique limits of normal levels of AFP in maternal blood have been worked out during pregnancy from a series of 3010 samples of blood. Comparing mean curves for the levels of AFP in mothers suffering from anaemia and diabetes with normal curves shows that there is a significant rise in these le... |
61,996 | B lymphocyte alloantigen specificities present on cultured lymphoblastoid cell lines. | Thirty-three of 34 cultured human lymphoblastoid B cell lines have been shown to express four out of five polymorphic non-HLA specificities expressed by normal B lymphocytes. The specificities were detected by 32 alloantisera produced by absorption with pooled platelets to remove HLA activity and selected from over 400... |
61,997 | Nucleoside specificity in the carrier IgG-dependent induction of tolerance. | Induction of tolerance to nucleoside haptens in BALB/c mice with isologous IgG conjugates bearing four nucleosides simultaneously (A, G, C, T)-IgG was confirmed. A mixture of separate nucleoside-IgG tolerogens (A-IgG, G-IgG, C-IgG, and T-IgG) was as effective or more effective that the (A, G, C,T)-IgG form in suppressi... |
61,998 | Nonspecific inhibition of tumor growth in vivo by admixed allogeneic tumor-sensitized lymphoid cells and identical inactivated allogeneic tumor cells. | With the in vivo tumor neutralization test (Winn test), growth of a transplanted (KMT-17) from Wistar-King-Aptekman rats was inhibited by allogeneic tumor (AH-66 from Donryu rats)-sensitized syngeneic lymphoid cells admixed with mitomycin C (MMC)-treated AH-66 cells. The observed tumor inhibition may be immunologically... |
61,999 | Release of chemical mediators from partially purified human lung mast cells. | Human lung mast cells dispersed by enzymatic digestion of human lung fragments were concentrated to greater than 50% purity by sedimentation in isopycnic and velocity gradients. The dispersed lung mast cells had a characteristic ultrasturctural appearance including granules with a scroll or reticular structural appeara... |
62,000 | Primary in vitro sensitization of murine lymphocytes against isogeneic and allogeneic cells transformed by simian virus 40. | Primary in vitro sensitization of murine lymphocytes to isogeneic and allogeneic cells transformed by simian virus 40 (SV40) is described. The results of specificity studies utilizing cytotoxic effector lymphocytes obtained by in vitro immunization indicate that SV40 transformation results in the expression of tumor-sp... |
62,001 | Characterization of antibodies to the structural polypeptides of HGSAg: evidence for subtype-specific determinants. | Antisera prepared in guinea pigs to the structural polypeptides of HBAAg/adw and HBSAg/ayw were examined by a modified passive hemagglutination assay for antibodies to the subtype-specific d and y determinants. All of the isolated polypeptide fractions stimulated antibodies to both group specific and subtype-specific a... |
62,002 | The demonstration of cell-associated immunity to viruses. In vitro lymphocyte responsiveness to Varicella-zoster antigen. | The demonstration of in vitro lymphocyte responsiveness to common pediatric viruses has previously been fraught with many technical and conceptual problems. Based upon our prior experience in demonstrating cell-associated immunity to mumps, rubella and measles viruses we illustrate our methodology and conceptual framew... |
62,004 | Measurement of the fractional uptake of macromolecules by the renal vascular bed compared to other vascular beds. | Macromolecules resembling soluble immune complexes can be made from heat-aggregated human gamma globulin (AHGG). In 15 rats, we studied vascular trapping of 125I-labeled AHGG (AHGG)-125I) given by constant I.V. infusion over 1 hour while tissue blood flow was marked by intermittant aortic arch injections of 85Sr-labele... |
62,005 | Wenckebach-type exit block from an ectopic focus as a cause of variable coupling. | Intermittent bigeminal and trigeminal ventricular premature beats were recorded in an otherwise healthy 14 year old male. Coupling intervals progressively lengthened until an ectopic beat was dropped. Odd numbers of sinus beats occurred between bigeminal runs. This rhythm is interpreted as being due to Wenckebach-type ... |
62,006 | Prostatic distribution of sex hormone-binding globulin and cortisol-binding globulin in benign hyperplasia. | Sex hormone-binding globulin-(SHBG) and cortisol-binding globulin-(CBG) like proteins have been demonstrated in prostatic tissue surgically removed from patients with benign prostatic hyperplasia. These proteins are not easily removed by superfusion of tissue slices. Epithelial tissue was separated from stroma and foun... |
62,007 | Metal-androgen interrelationships in carcinoma and hyperplasia of the human prostate. | Zinc and cadmium concentrations were measured by atomic absorption spectroscopy in normal and pathological human prostates. Our studies confirm the values of zinc in normal tissue [6.84 +/- 1.21 (S.E.M.) mumol/g] and benign prostatic hypertrophy (BPH) (6.9 +/- 1.19 mumol/g) are similar, while in neoplastic tissues zinc... |
62,008 | Androgen levels in the plasma and prostatic tissues of patients with benign hypertrophy and carcinoma of the prostate. | Specific radioimmunoassays for testosterone, dihydrotestosterone (DHT) and androstenedione were carried out to measure the concentrations of the three hormones in the plasma and prostatic tissue of ten patients with benign prostatic hypertrophy (BPH) and ten patients with carcinoma of the prostate. The results indicate... |
62,009 | Immunological studies of aging. II. Loss of IgG and high avidity plaque-forming cells and increased suppressor cell activity in aging mice. | The magnitude and heterogeneity of the immune response to dinitrophenylated bovine gamma globulin was measured in aged and young mice at a cellular level using an inhibition of plaque-forming cell assay. The primary and secondary responses of 24-mo-old mice were markedly depressed in magnitude and restricted in avidity... |
62,010 | Alternative pathway of complement: demonstration and characterization of initiating factor and its properdin-independent function. | A novel component of the properdin system has been discribed which represents a heretofore unrecognized human serum protein. The protein has been tentatively termed the initiating factor (IF) because it functions in the initial reaction of the properdin pathway. IF is a 170,000 dalton beta-pseudoglobulin which is compo... |
62,011 | Ly phenotype of cytotoxic T cells for syngeneic tumor. | Our present and previous findings may be summarized as follows: The phenotype of C57BL/6 (B6) cytotoxic cells for allogeneic target cells is Thy-1+, Ly-1- Ly-2/3+, MSLA+, and Ig-. the phenotype of B6 cytotoxic cells for syngeneic tumor cells is Thy-1+, Ly-1+, Ly-2/3+, MSLA+, and Ig-. The phenotype of B6 cytotoxic cells... |
62,012 | Independent differentiative pathways of Ly1 and Ly23 subclasses of T cells. Experimental production of mice deprived of selected T-cell subclasses. | When B mice are supplied with Ly1 or Ly23 cells they acquire, over the next 6 mo, only the immune functions associated with each of these T-cell subclasses, respectively. The T-cell population of these "B-Ly1" and "B-Ly23" mice mice also remains restricted to the Ly1 and Ly23 subclass phenotypes. Thus the Ly1 and Ly23 ... |
62,013 | Cell-mediated lympholysis to H-2-matched target cells modified with a series of nitrophenyl compounds. | The specificity of C57BL/10 cytotoxic effector cells generated by in vitro sensitization with autologous spleen cells modified with a series of related nitrophenyl compounds was investigated. The failure of trinitrophenyl (TNP)-sensitized effector cells to lyse TNP-beta-alanylglycylglycyl(AGG)-modified target cells is ... |
62,014 | Effects of anti-Ia sera on mitogenic responses. III. Mapping the genes controlling the expression of Ia determinants on concanavalin A-reactive cells to the I-J subregion of the H-2 gene complex. | We have shown that the Ia determinants expressed on nylon wool-purified T lymphocytes reactive to concanavalin A (Con A) in serum-free media are coded in a single I subregion of the H-2 gene complex. This region, I-J, is defined by two pairs of intra-H-2 recombinant haplotypes: H-2t3, H-2t4 and H-2i3, H-2i5, carried by... |
62,015 | Serological detection of variable region (Vh) subgroups of Ig heavy chains. | Serological test systems were established for determining the heavy-chain variable region (Vh) subgroups of immunoglobulin heavy chains. Myeloma proteins with known Vh subgroups based on amino acid sequence were utilized as the primary basis of reference for analysis by hemagglutination and hemagglutination inhibition.... |
62,016 | H-2 restriction of virus-specific cytotoxicity across the H-2 barrier. Separate effector T-cell specificities are associated with self-H-2 and with the tolerated allogeneic H-2 in chimeras. | During infection with lymphocytic choriomeningitis or vaccinia virus, F1 irradiation chimeras reconstituted with bone marrow cells from or both parents generate cytotoxic T cells which can lyse targets across the H-2 barrier. However, activity of chimera T cells is H-2 restricted as shown by cold target competition exp... |
62,017 | Suppression by autogenous complementary idiotypes: the priority of the first response. | Complementary idiotypes or antibodies are considered to have combining site structures which are at least partly directed against each other. Complementary antibodies were induced in A/He mice by immunization with phosphorylcholine (PC)-containing antigens and by immunization with the PC-binding IgA myeloma protein TEP... |
62,018 | Anti-idiotype sera raised against surface immunoglobulin of human neoplastic lymphocytes. | The idiotypic determinants of surface immunoglobulins on B-cell lymphomas and lymphocytic leukemias represent tumor-specific antigens, individually unique for each tumor. As such they have both diagnostic and therapeutic potential, particularly for those neoplasms with no serum monoclonal immunoglobulin arising from sy... |
62,019 | Lysis of RNA tumor viruses by human serum: direct antibody-independent triggering of the classical complement pathway. | In earlier studies we found that human serum, but not serum from multiple other species, inactivated and lysed oncornaviruses from a number of diverse sources in the apparent absence of antibody. A detailed analysis of the role of the human complement (C) system in mediating this lytic process indicates that human C1q ... |
62,020 | The analysis of the monoclonal immune response to influenza virus. II. The antigenicity of the viral hemagglutinin. | The antigenicity of the hemagglutinins (HA) of five influenza viruses of the A0 and A1 subtypes has been analyzed by means of monoclonal antibodies of murine origin produced in vitro. Secondary monoclonal anti-HA(PR8) antibodies were able to differentiate 14 antigenic determinants (or groups of determinants) on the HA ... |
62,022 | Some characteristics of salt-dependent haemagglutinating measles viruses. | Several strains of measles virus which did not agglutinate monkey erythrocytes in phosphate-buffered saline did so in buffer containing 0-8 M-ammonium sulphate. Haemadsorption to cells infected with these viruses was also salt-dependent. In a series of tests salt-dependent agglutinin was shown to be a stable structural... |
62,023 | Tryptic cleavage of antibody binding sites from hepatitis B surface antigen particles. | The sedimentation of radiolabelled 22 nm hepatitis B surface antigen particles was unaffected by treatment with either trypsin or SDS alone, but combined treatment disrupted the particulate nature of the radiolabelled material. Considerable antibody binding activity by the group-specific determinant (a) was preserved a... |
62,024 | A comparison of human papovavirus T antigens. | A comparison was made of the T antigens induced in transformed cells or infected permissive cells by representatives of three categories of human papovavirus. The transformed hamster cell lines employed contained T antigen induced by either the BK or RF strains of papovavirus associated with human renal allografts; the... |
62,025 | Evaluation of the blood-CSF barrier by protein gradients and the humoral immune response within the central nervous system. | A linear correlation was found between the serum/cerebrospinal fluid (CSF) concentration ratios of albumin, caeruloplasmin and alpha2-macroglobulin and their hydrodynamic radii in a semilogarithmic plot. This protein gradient is used as a parameter to evaluate the blood-CSF barrier under normal and pathological conditi... |
62,026 | Generalized gangliosidosis: acid beta-galactosidase deficiency with early onset, rapid mental deterioration and minimal bone dysplasia. | This report concerns a 3-month-old girl with rapidly progressive psychomotor retardation, hepatomegaly, vacuolated lymphocytes, minimal bone dysplasia and normal excretion of acid mucopolysaccharides. A deficiency of acid beta-galactosidase was demonstrated in isolated leucocytes and in a liver biopsy. The diagnosis of... |
62,027 | [Studies on the circadian periodicity in patients with the awakening type of idiopathic epilepsy (author's transl)]. | In 6 patients with idiopathic epilepsy of the awakening type and 3 control subjects, the heart rate, body temperature and urinary excretion of sodium, potassium and calcium were measured over 72 h. The patients and the control subjects stayed in the hospital under constant environmental conditions including a standard ... |
62,028 | Ultrastructural investigations of peripheral nerves in neuronal ceroid-lipofuscinoses (NCL). | Specimens of brachial plexus, sural nerve and two cranial nerves of one patient with Jansky-Bielschowsky type and 3 patients with the Spielmeyer-Sjögren type of NCL were studied by electron microscopy. Significant light microscopic changes were absent in all specimens. Ultrastructurally, curvilinear and/or fingerprint ... |
62,029 | Myotonic dystrophy and multiple sclerosis. | A 48-year-old man with myotonic dystrophy did well until the age of 37, when he developed the first of many remissions and exacerbations of multiple sclerosis. The only serum value done while the patient was infection free, revealed serum hypogammaglobulinemia. Three determinations of the relative concentration of gama... |
62,030 | Intrathecal cytostatic therapy of meningeal carcinomatosis. Autoradiographic investigations of the CSF cells. | Autoradiographic investigations with 3H-thymidine were performed on cerebrospinal fluid (CSF) cells from a case of meningeal carcinomatosis following carcinoma of the breast. The cells were found to be anaplastic histologically. Within a period of 12 days 3 X 25 mg methotrexate were injected into the subarachnoid space... |
62,032 | Function and innervation of the involuntary m. retroauricularis. | Beside the automatic, obligatory and tonic coinnervation of the involuntary m. retroauricularis in conjugate lateral gaze (oculoauricular phenomenon, nystagmus) several other physiological ways of accidental coinnervation are described. In talking, chewing, swallowing and during involuntary inspiration irregular bursts... |
62,031 | [N. interosseous anterior syndrome. Study in 4 cases of our own and in 49 cases from the literature (author's transl)]. | The anterior interosseous nerve branches off from the median nerve distal of the pronator radii teres. It lies on the interosseous membrane, always innervates the flexor pollicis longus, usually the portion of the deep flexor belonging to the second, sometimes also the portion belonging to the third finger, then runs t... |
62,034 | The anterior interosseous nerve syndrome. | A case of isolated anterior interosseous nerve palsy with its characteristic clinical picture of lack of function is described. The typical symptoms are the inability to flex the terminal phalanges of the thumb and index finger, possibly of other fingers too, and the inability to pronate the forearm when the elbow is f... |
62,033 | Genetic investigations on chronic forms of infantile and juvenile spinal muscular atrophy. | A material of 247 cases selected from 260 cases of spinal muscular atrophy in the Warsaw Department of Neurology in 1960-1974 was analyzed. The size of sibships was established and calculations were made of the mean distribution of the age at onset, also according to sex, for the different clinical forms, genetical pro... |
62,035 | Plasma membranes of muscle in experimental myotonia in rats. | Determinations of protein and phospholipid composition, as well as enzymatic activity, were carried out in plasma membranes isolated from the muscle of rats, after different periods of 20,25-diazacholesterol administration. A decrease in the level of phospholipids, and in the total amount of plasma membrane proteins, c... |
62,036 | Sarcoplasmic reticulum in experimental myotonia in rats. | Determinations of enzymatic activity, protein structure and phospholipid composition of sarcoplasmic reticulum isolated from the soleus muscle (S), extensor digitorum longus muscle (EDL) and gastrocnemius muscle (G) in rats were carried out after various periods of 20,25-diazacholesterol administration. The sarcoplasmi... |
62,037 | Measles virus infection and multiple sclerosis: serological studies. | In 159 patients out of 161 with multiple sclerosis (MS), a significant rise in the level of measles hemagglutination inhibition (HI) antibody was found in the serum and in 92 MS patients the occurrence of measles HI antibody in the CSF was significantly more frequent. MS patients showed CSF humoral response against mea... |
62,039 | Anatomical analysis of ventrolateral thalamic input to primate motor cortex. | 1. The origin and topographical organization of input to the arm area of the primate motor cortex from the ventrolateral thalamus were examined using the method of retrograde transport of horseradish peroxidase (HRP). 2. A thin, continuous slab of labeled neurons was found in the ventrolateral thalamus followingmultipl... |
62,040 | A method of integrating family nursing in an undergraduate curriculum. | The authors describe integrating theoretical content of families into an undergraduate curriculum based on the biopsychosocial spheres. Using audiovisual materials, and printed media, the student is introduced to independent learning and applies content to clinical settings in the community. |
62,042 | A plasma glycoprotein depressed in vitamin A deficiency in the rat: alpha 1-macroglobulin. | Plasma glycoprotein synthesis in normal and vitamin A-deficient rats was investigated by injecting the rats with labeled carbohydrate precursors and then fractionating their plasmas on DEAE-Sephadex. Plasma from deficient rats showed a consistent depression of 30% in the uptake of label into a peak eluting with 0.23 M ... |
62,043 | Topographical distribution of sulphated glycosaminoglycans in human temporomandibular joint disks. A histochemical study of an autopsy material. | The distribution of sulphated glycosaminoglycans (GAG's) in the human temporomandibular joint disk and its relationship to sex, age and osteoarthrosis was studied by histochemical methods in autopsy material from 18 individuals. The disks were embedded in paraffin and frontal sections, 5-7 mu thick, were cut at differe... |
62,044 | Chondromyxoid fibroma of the mandible. | A new case of chondromyxoid fibroma of the jaw arising in a 16-year-old white girl is presented. It is possible that unrepresentative biopsy specimens of this condition could be misdiagnosed as myxofibroma, chondrosarcoma, or mesenchymal chondrosarcoma. |
62,046 | An experimental kidney disease in dogs produced by injection of heterologous antisera to dog tubular fraction 3 antigen. | Glomerular disease characterised by morphological alterations of the glomerular basement membrane and the mesangium was produced in dogs by injections of heterologous anti-dog renal tubular fraction 3 antibody. The renal lesion was characterised by irregular thickening of the glomerular basement membrane, with electron... |
62,047 | Exeriences with isoprenaline induced myocardial necrosis in the rat. | An attempt has been made to quantify myocardial lesions produced in the rat by isoprenaline for use as a model to assess possible incremental effects of environmental and dietary factors. This was initially made difficult by variation in the cardiotoxicity of different samples of isoprenaline. Investigation of these sa... |
62,049 | Comparison of graphical and computerized methods for calculating binding parameters for two strongly bound drugs to human serum albumin. | The determination of drug-protein binding parameters (n's and K's) can lead to important information on the required therapeutic dosage regimen and possible clinical complications associated with competitive displacement of one drug by a concurrently administered agent. Graphical and computer estimates of the data are ... |
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