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Video Comment on Michel Kahaleh et al.	{ "pile_set_name": "PubMed Abstracts" }
Physical and psychological maltreatment: relations among types of maltreatment. Maltreatment has serious consequences for the development of children. The reason for the negative outcomes is not, however, fully understood. This study investigated the hypotheses that psychological maltreatment would be present in almost all cases of physical maltreatment and that it would be more related to detrimental outcomes for children than would severity of injury. A sample of 175 maltreated children, 39 children in mental health treatment, and 176 normative children was assessed for type and severity of maltreatment. Both hypotheses were supported. In addition, evidence is provided that psychological maltreatment can occur alone, that assessments of parental psychologically maltreating behavior and negative child outcomes are highly correlated, and that child age and gender are unrelated to psychological maltreatment in young children whereas family income is related. Implications for investigation and treatment are considered.	{ "pile_set_name": "PubMed Abstracts" }
The influence of laparoscopy on incisional hernia rates: a retrospective analysis of 1057 colorectal cancer resections. The aim of this study was to evaluate the incidence of incisional hernia formation after laparoscopic and open surgery for colorectal cancer. A retrospective analysis was conducted of 1057 colorectal cancer resection cases (289 laparoscopic, 768 open) performed in a single national laparoscopic training centre between January 2006 and December 2011. Clinical notes and serial computed tomography scans were reviewed, with any incisional hernia including those at a surgical incision, port site, stoma and stoma closure site identified and the size of the defect measured. The overall incisional hernia rate was 14.8%. There was no significant difference between the open and laparoscopic groups (14.4% vs 15.9%, P = 0.566). Excluding stoma-related hernia, 10.7% of the open group developed a surgical wound hernia, and 11.1% of the laparoscopic group developed a hernia at a port site, extraction site or surgical midline incision. There was no statistical difference between the two groups (P = 0.853). The defects were smaller in the laparoscopic group (P < 0.005). There were significantly more parastomal hernias in the laparoscopic group (40%) than in the open group (12.7%, P < 0.001). The incidence of incisional hernia formation was similar after laparoscopic or open surgery for colorectal cancer. Parastomal hernia was more frequent after laparoscopic surgery.	{ "pile_set_name": "PubMed Abstracts" }
Lymphocyte populations in tuberculous pleural effusions. Different systemic and local responses to mycobacterial antigens suggest an active compartmentalization of responsive lymphocytes to tubercular antigens. This fact, observed in pleuritic processes, raises doubts about the accuracy of information obtained in the study of cells taken solely from peripheral blood. For this reason we decided to study the concept of compartmentalization in 140 patients suffering from pleural effusions. Patients were classified into six groups according to the aetiology of the effusion: group I, tuberculous, n = 23; group II, paraneoplastic, n = 41; group III, metapneumonic empyematous, n = 5; group IV, transudate, n = 38; group V, miscellaneous exudate, n = 19; group VI, unknown aetiology, n = 14. In each group we studied the lymphocyte population by using flow cytometry with doubly fluorescent monoclonal antibodies: B [expressing human lymphocyte antigen (HLA)-DR on the surface], T (CD3+), CD4+ and CD8+, and the subpopulation of activated T lymphocytes (together expressing CD3 and HLA-DR on the surface) (CD3+DR+). The study of these subpopulations in peripheral blood did not yield valuable results, but the CD3+DR+ population in pleural fluid demonstrated a diagnostic efficiency of 84% [positive predictive value (PPV) 51%, negative predictive value (NPV) 96%] at a cut-off value of 80.4 cells/mm3. The CD3+DR+ pleural fluid/peripheral blood ratio demonstrated an efficiency of 83% (PPV 50%, NPV 96%), and showed a statistically significant difference (P < 0.02) with regard to all the diagnostic groups, with the exception of the paraneoplastic effusions. The lymphocytic subpopulations study confirms the concept of compartmentalization in tuberculous pleuritis, as shown by the greater number of activated T lymphocytes present in pleural fluid in comparison with peripheral blood in tuberculous pleuritis, a 98% efficiency of adenosine deaminase (ADA) determination in pleural fluid versus a 50% value in peripheral blood, predominance of helper cells (CD4+) in pleural fluid and suppressor cells (CD8+) in peripheral blood, a greater CD4+/CD8+ ratio in pleural fluid than in peripheral blood, and a significant correlation of ADA-CD3+DR+ in pleural fluid, which does not occur in peripheral blood.	{ "pile_set_name": "PubMed Abstracts" }
Stimulation of insulin and glucagon secretion by vasoactive intestinal peptide. In vivo, vasoactive intestinal peptide (VIP) produces simultaneous increases in blood glucose and insulin levels. In order to determine whether VIP, like its homologues, also stimulates insulin secretion directly, studies were made in controlled glucose media employing the vascularly perfused cat pancreas. VIP stimulated insulin secretion significantly in the presence of constant physiological concentrations of glucose. The highest insulin response to VIP (100.3+/-8.1 muU/min) approached the highest insulin response to glucose (119.9 +/- 12.0 muU/min). In the absence of glucose, the insulin response to VIP was insignificant. Unexpectedly, VIP was found to be a more effective stimulant of glucagon than of insulin secretion. The highest glucagon response to VIP (327+/-51% of control levels) was attained in the presence of physiological concentrations of glucose and equalled the glucagon response obtained upon withdrawal of glucose from the perfusate. The glucagon response to VIP was blocked by increasing the glucose in the perfusate. These studies indicate the VIP present in pancreatic islets might play a role in the local control of pancreatic endocrine function.	{ "pile_set_name": "PubMed Abstracts" }
An automatic device for forced wakefulness in dogs. An automatic device is described which maintains sustained wakefulness of 12 h or more in dogs with chronically implanted electrodes for sleepwaking recording. The procedure consists of automatic sleep detection using EEG, EMG and body movement (BM), and of avoidance conditioning to arouse the animal with sound as conditioned stimulus, and with electroshock as unconditioned stimulus. The procedure does not disturb feeding, drinking and freely moving, and is not associated with forced exercise, or with a marked stress reaction as measured with cortisol and growth hormone. These advantages enable us to maintain 12 h of forced wakefulness in the same animal for 7 or more consecutive days.	{ "pile_set_name": "PubMed Abstracts" }
Children teach methods they could not discover for themselves. Across three studies (N=100), we explored whether and, if so, under what circumstances children's self-discovered knowledge impacts their transmission of taught information. All participants were taught one of several methods for extracting rewards from a box. Half of the participants were also given an opportunity to discover their own method prior to receiving such instruction. Across studies, we varied the transparency of the taught method relative to the method children could discover on their own. When asked to teach a naive pupil about the box, children who did not explore the box always transmitted what they were taught. Children in the Exploration+Instruction condition were also likely to transmit what they had been taught, but they were especially likely to do so when the taught method was more opaque than the method they had discovered for themselves. Thus, children faithfully transmit what they have been taught, but only when that information is difficult to discover.	{ "pile_set_name": "PubMed Abstracts" }
To split behaviour into bouts, log-transform the intervals. Analysis of behaviour that is displayed in bouts depends crucially on quantitative estimates of bout criteria, that is, the lengths of the shortest intervals between bouts. Current methods estimate bout criteria by modelling the log-transformed (cumulative) frequency distributions of intervals between events. For analysis of feeding behaviour, these models will not result in biologically meaningful quantitative estimates (Tolkamp et al. 1998, Journal of Theoretical Biology194, 235-250). We proposed a method that models the frequency distribution of log-transformed interval lengths instead. Applying this method to a single data set showed that the log-transformed lengths of intervals between feeding events were distributed as two Gaussians. Here we test this model using a data set of 35 171 intervals between feeding that was obtained during an experiment with 38 cows in three dietary treatment groups. No meaningful bout criterion could be obtained for some individuals, which casts doubt on the general validity of the proposed model. Addition of a third log-normal improved the fit of the model and we hypothesized that this third population represents intervals including drinking. In a second experiment, we found the measurements to be consistent with this hypothesis. We obtained meaningful meal criteria for all individuals by fitting either a double, or a triple, log-normal model to the frequency distributions of the lengths of intervals between feeding. These log-normal models appear to be not only more biologically meaningful than log (cumulative) frequency models but also far more flexible. Copyright 1999 The Association for the Study of Animal Behaviour.	{ "pile_set_name": "PubMed Abstracts" }
Novel insights into the immune regulatory effects of ferritins from blunt snout bream, Megalobrama amblycephala. Ferritins play vital roles in maintenance of iron homeostasis as iron storage proteins. Recently, the immune function of ferritins have attracted increasing attention, especially their roles in defense against pathogenic infections. However, the immune regulatory mechanism of fish ferritins are not well known. In the present study, comparative digital gene expression (DGE) profiling was performed to explore the regulatory effects of the Megalobrama amblycephala ferritins (MamFers) using MamFers overexpressed and control L8824 cells (Ctenopharyngodon idella hepatic cell line). Clean reads were aligned to the C. idella genome and differential expression analysis was conducted with representative differentially expressed genes pointed out. On that basis, further studies were performed to verify two pivotal regulated pathways in L8824 and EPC (Epithelioma Papulosum Cyprini cell line) cells, respectively. The results showed that NLRC5 (NOD-like Receptor Family CARD Domain Containing 5) mediated the regulation of MamFers on expression of MHC I (Major Histocompatibility Complex Class I) and its chaperone β2M (Beta-2-Microglobulin) in L8824 cells. Then, β2M further mediated the regulation of MamFers on hepcidin expression, indicating that MamFers regulated the expression of hepcidin via NLRC5/MHC I/β2M axis. In addition, MamFers regulated the adhesion of Aeromonas hydrophila to EPC cells by regulating the expression of two extracellular matrix proteins Intgβ1 (integrin β1) and FN (fibronectin). In a word, the present study provided novel insights into the immune regulatory functions of fish ferritins.	{ "pile_set_name": "PubMed Abstracts" }
Pointed Progress in Second-Line Advanced Non-Small-Cell Lung Cancer: The Rapidly Evolving Field of Checkpoint Inhibition. Non-small-cell lung cancer (NSCLC) is globally prevalent and associated with high rates of mortality. Immune checkpoint pathways are often exploited by tumors to evade immunity-mediated destruction, and checkpoint inhibitors can reactivate tumor-related immune responses. This review considers available clinical evidence for the use of checkpoint inhibitors in the treatment of second-line advanced NSCLC. Our systematic search revealed 20 clinical trials evaluating checkpoint inhibitors in the second-line setting, three of which were randomized trials comparing programmed cell death protein 1 and programmed death ligand 1 (PD-L1) inhibitors to docetaxel, the current standard of care in this setting. A randomized phase II trial comparing the PD-L1 inhibitor atezolizumab to docetaxel did not demonstrate improved survival for atezolizumab in patients overall, although a trend toward improved survival with increased PD-L1 expression was apparent. Twin phase III trials showed significantly improved survival for the programmed cell death protein 1 inhibitor nivolumab compared with docetaxel in patients with both squamous and nonsquamous disease. PD-L1 expression correlated with improved survival in patients with nonsquamous disease, and patients with low levels of PD-L1 expression (< 10%) and those with EGFR mutations are unlikely to benefit. Checkpoint inhibitor therapy is generally well tolerated and associated with low rates of grade 3 or 4 adverse events compared with standard care. Level 1 evidence exists to support the use of nivolumab as second-line treatment of patients with squamous advanced NSCLC, as well as in select patients with nonsquamous disease. Benefits remain unknown in patients with targetable driver mutations, and use of PD-L1 expression to guide therapy remains controversial. Results from ongoing randomized trials evaluating biomarkers and other checkpoint inhibitors will further our understanding of this rapidly evolving area of oncology.	{ "pile_set_name": "PubMed Abstracts" }
A new technique for the mapping of oxygen tension on the brain surface. Most measurements of oxygen tension (PO(2)) in the brain have been performed using oxygen microelectrodes. However, the insertion of microelectrodes into the brain per se causes cortical injury and hence could lead to erroneous PO(2) measurements. The recently developed "quenching lifetime method" requires the injection of fluorescent chemicals into the blood circulation. To address this issue, we tested the feasibility of our O(2)-sensitive fluorescent membrane technique in the rat brain, and visualized the spatial distribution of PO(2) on the brain surface as epifluorescent microscopic patterns. An O(2)-quenching fluorescence dye, tris (1,10-phenanthroline) Ru(2+), was immobilized in a highly gas-permeable, thin silicone-rubber film formed on a microscope coverslip. Unlike the original method, which was intended for transparent rat mesenteric tissue, any change in the redox state in the brain tissue will influence the optical measurement of PO(2). Thus, in the present study, the O(2)-sensing membrane was further coated with a thin opaque silicone-rubber to minimize this type of influence. This new method enabled us to visualize the PO(2) gradient on the rat brain without causing cortical injuries. In an ischemia/reperfusion model using Pulsinelli's four-vessel occlusion rats, the changes in the PO(2) were highly heterogeneous during the ischemic period and this heterogeneity, both temporal and spatial, was higher in the off-arteriolar area than in the peri-arteriolar area.	{ "pile_set_name": "PubMed Abstracts" }
The fidelity of protein synthesis: can mischarging by aspartyl-tRNA(Asp) synthetase lead to the formation of isoaspartyl residues in proteins? We have tested the hypothesis that isoaspartic acid residues in proteins can arise via errors that occur during protein synthesis. One such error involves a mischarging step in which the aspartic acid side-chain beta-carboxyl group is linked to the tRNA(Asp) instead of the main chain alpha-carboxyl group. If this altered Asp-tRNA(Asp) is a substrate for the ribosomal elongation reactions, a polypeptide will be made with an isoaspartic acid, or beta-linkage, in which the peptide chain is branched at the side chain of the aspartic acid residue. Using an ammonium sulfate fraction of aspartyl-tRNA(Asp) synthetase from Escherichia coli and [3H]aspartic acid, we have prepared [3H]aspartyl-tRNA(Asp) complexes and directly analyzed the linkage of the [3H]aspartate to the tRNA by identifying the products of ammonolysis. Normal attachment of the alpha-carboxyl group of aspartate to the tRNA produces [3H]isoasparagine, while the mischarging reaction leads to [3H]asparagine formation after ammonolysis. We have separated [3H]isoasparagine from [3H]asparagine and found an upper limit of 1 asparagine per 10,000 isoasparagines. These results show that the bacterial aminoacyl-tRNA synthetase can very accurately distinguish between the alpha- and beta-carboxyl groups of aspartic acid and suggest that only a very small fraction of the isoaspartic acid residues found to occur in cellular proteins may be the result of mischarging steps.	{ "pile_set_name": "PubMed Abstracts" }
SEX-RATIO MANIPULATION IN RESPONSE TO HOST SIZE BY THE PARASITOID WASP SPALANGIA CAMERONI: A LABORATORY STUDY. The prediction of Charnov et al.'s (1981) host-size model that there should be a negative relationship between host size and wasp sex ratio (proportion sons) was supported for Spalangia cameroni, a solitary parasitoid wasp. The relationship was shown to be a result of offspring sex manipulation by females in response to host size rather than a result of differential mortality of the sexes. A major assumption of the host-size model is that host size has a greater effect on the ultimate reproductive success of emerging female wasps than of males. This assumption was not supported. Host size had a positive effect on the size of both male and female S. cameroni. However, neither host size nor wasp size affected longevity, production of offspring by females, or ability of males to compete for mates. Host size may differentially affect the reproductive success of female and male wasps through effects on other aspects of reproductive success. Tests of the assumptions of offspring sex-ratio manipulation hypotheses are scarce but critical, not only for parasitoid wasps, but also for other organisms.	{ "pile_set_name": "PubMed Abstracts" }
Genetic polymorphisms of human mitochondrial glutamic oxaloacetic transaminase. In a survey of 860 unselected human placental extracts, three variants of mitochondrial glutamic oxaloacetic transaminase were found, all of which were common enough to be considered polymorphisms. Family studies showed that this enzyme is under the control of nuclear rather than mitochondrial DNA.	{ "pile_set_name": "PubMed Abstracts" }
Chimeric creatures in Greek mythology and reflections in science. "The Chimaera" in Homer's Iliad, "was of divine stock, not of men, in the forepart a lion, in the hinder a serpent, and in the midst a goat, ellipsis Bellerophon slew her, trusting in the signs of the gods." In Hesiod's Theogony it is emphasized that "Chimaera ellipsis had three heads, one of a grim-eyed lion, another of a goat, and another of a snakeellipsis". In addition to this interspecies animal chimera, human/animal chimeras are referred to in Greek mythology, preeminent among them the Centaurs and the Minotaur. The Centaurs, as horse/men, first appear in Geometric and early Archaic art, but in the literature not until early in the fifth century B.C. The bullheaded-man Minotaur, who is not certainly attested in the literary evidence until circa 500 B.C., first appears in art about 650 B.C. Attempts, in the fourth century B.C. and thereafter, to rationalize their mythical appearance were in vain; their chimeric nature retained its fascinating and archetypal form over the centuries. Early in the 1980s, experimental sheep/goat chimeras were produced removing the reproductive barrier between these two animal species. Late in the 1990s, legal, political, ethical, and moral fights loomed over a patent bid on human/animal chimeras. Chimeric technology is recently developed; however, the concept of chimerism has existed in literary and artistic form in ancient mythology. This is yet another example where art and literature precede scientific research and development.	{ "pile_set_name": "PubMed Abstracts" }
Effect of mild malnutrition on disaccharidase activity and glucose uptake in intestinal brush border vesicles of growing monkeys. The effect of severe malnutrition has been reported by some workers, but there is a paucity of information on milder forms even though 85% of pre-school children show evidence of mild to moderate malnutrition in India. Mild to moderate protein energy malnutrition was induced in 12 monkeys, and 12 were kept as controls. After a 30-40% reduction in body weight as compared with their controls, the animals were killed, and brush border membrane vesicles were prepared from three small parts of the small intestine. Purified vesicles were used for the assay of lactase, sucrase, maltase and glucose uptake in vitro. The activity of lactase, sucrase, and maltase, and uptake of (U-14C)-D-glucose was decreased in all three parts of small intestine, and the lactose tolerance test result was also abnormal in the protein energy-malnourished group. On nutritional rehabilitation, the disaccharidase activities recovered completely. This study indicates that even mild to moderate malnutrition decreases the digestion and absorption of carbohydrates throughout the small intestine, and it should not be ignored during the developing stage.	{ "pile_set_name": "PubMed Abstracts" }
DNA sequence analysis of the cytosolic acetaldehyde dehydrogenase gene (Ahd-2) in mouse strains with variable ethanol preferences. Differences in Ahd-2 at the DNA sequence level were characterized in mouse strains with variable ethanol preferences. The 5' region and the region surrounding the active site of Ahd-2 were compared to detect differences which could affect ethanol sensitivity. Only minor differences were found among the strains in the two regions. These differences cannot explain their variable ethanol preference and the implications of sequence identities among the divergent strains in these regions has yet to be determined.	{ "pile_set_name": "PubMed Abstracts" }
A quality assurance test tool for high dose-rate remote afterloading brachytherapy units. A QA test tool is designed to quantitatively measure the HDR source positioning error, and to facilitate quick and dependable HDR timer linearity test and daily output constancy check. The test tool consists of two concentric disks. The lower disk has a cutout for inserting an HDR catheter, and the upper disk accepts a diode or miniature ionization chamber and can rotate relative to the lower disk. Ionization readings from the source (transferred to the center of the disks) are obtained at two rotational positions of the upper disk which houses the detector. The ratio of the readings is used to determine the source-positioning error of the HDR unit relative to the nominal source position by a simple triangulation principle. Experimental measurements confirm that the QA test tool is sensitive to approximately 0.2 mm variance in source positioning errors. In addition, the QA test tool is suitable for other common HDR QA tests such as the source travel step size test, the daily HDR unit output constancy check, and the timer linearity test. Its simple and robust design permits routine clinical use and provides a high confidence level in the accurate operation of HDR units.	{ "pile_set_name": "PubMed Abstracts" }
Asymptomatic sustained ventricular fibrillation in a patient with left ventricular assist device. Optimal medical treatment, cardiac resynchronization, and the use of an implantable cardioverter defibrillator are established therapies of severe congestive heart failure. In refractory cases, left ventricular assist devices are more and more used not only as bridging to cardiac transplantation but also as destination therapy. Ventricular arrhythmias may represent a life-threatening condition and often result in clinical deterioration in patients with congestive heart failure. We report a case of asymptomatic sustained ventricular fibrillation with preserved hemodynamics caused by a nonpulsatile left ventricular assist device. Consecutive adequate but unsuccessful discharges of the implantable cardioverter defibrillator were the only sign of the usually fatal arrhythmia, prompting the patient to consult emergency services. Electrolyte supplementation and initiation of therapy with amiodarone followed by external defibrillation resulted in successful restoration of a stable cardiac rhythm after 3.5 hours.	{ "pile_set_name": "PubMed Abstracts" }
The preoperative patient with an asymptomatic cervical bruit. At the time of this writing there appears to be little evidence that cervical bruits are good indicators of stenosis or plaque in the underlying carotid artery in asymptomatic patients, or that the finding of a carotid bruit places the patient at greater risk of postoperative stroke. The mortality and morbidity of angiography and endarterectomy are too high to risk their use in a group of asymptomatic patients.	{ "pile_set_name": "PubMed Abstracts" }
Effectiveness of Preceding Solo Kidney Transplantation for Type 1 Diabetes With End-Stage Renal Failure. Preceding solo kidney transplantation for type 1 diabetes with end-stage renal failure is controversial because of less pancreatic graft survival in pancreas transplantation after kidney transplantation (PAK) than in simultaneous pancreas and kidney transplantation (SPK). To study the effectiveness of preceding solo kidney transplantation for type 1 diabetes with end-stage renal failure, comparative retrospective analysis was performed between SPK (n = 232) and PAK (n = 39) that were performed until December 2016. At 1, 3, and 5 years, pancreatic graft survival in SPK was 87.5%, 86.4%, and 82.8%, respectively, and 87.1%, 65.0%, and 49.1%, respectively, in PAK, which showed lesser long-term graft survival than SPK. Because 10 cases out of 16 (62.5%) failed into pancreatic graft loss with rejection in PAK, which was about 3 times more than in SPK, control of rejection is very important; rejection episodes were decreased by rabbit antithymocyte globulin induction resulting in improved graft survival. Five-year patient survival was 88.0% in SPK and 96.6% in PAK. Considering patient survival, preceding solo kidney transplantation for type 1 diabetes with end-stage renal failure should be performed if a donor is available.	{ "pile_set_name": "PubMed Abstracts" }
Inhibiting Stearoyl-CoA Desaturase Ameliorates α-Synuclein Cytotoxicity. The lack of disease-modifying treatments for neurodegenerative disease stems in part from our rudimentary understanding of disease mechanisms and the paucity of targets for therapeutic intervention. Here we used an integrated discovery paradigm to identify a new therapeutic target for diseases caused by α-synuclein (α-syn), a small lipid-binding protein that misfolds and aggregates in Parkinson's disease and other disorders. Using unbiased phenotypic screening, we identified a series of compounds that were cytoprotective against α-syn-mediated toxicity by inhibiting the highly conserved enzyme stearoyl-CoA desaturase (SCD). Critically, reducing the levels of unsaturated membrane lipids by inhibiting SCD reduced α-syn toxicity in human induced pluripotent stem cell (iPSC) neuronal models. Taken together, these findings suggest that inhibition of fatty acid desaturation has potential as a therapeutic approach for the treatment of Parkinson's disease and other synucleinopathies.	{ "pile_set_name": "PubMed Abstracts" }
Methyldopa-induced immune hemolytic anemia and chronic active hepatitis. Immune hemolytic anemia and chronic active hepatitis developed concurrently in a 76-year-old man after prolonged therapy with methyldopa. Both complications disappeared following cessation of treatment with the offending drug and a short course of a high-dose corticosteroid (prednisone). Inhibition of T-suppressor lymphocyte function by methyldopa may be instrumental in the emergence of autoimmune complications following usage of the drug.	{ "pile_set_name": "PubMed Abstracts" }
An Algorithm for Motif Discovery with Iteration on Lengths of Motifs. Analysis of DNA sequence motifs is becoming increasingly important in the study of gene regulation, and the identification of motif in DNA sequences is a complex problem in computational biology. Motif discovery has attracted the attention of more and more researchers, and varieties of algorithms have been proposed. Most existing motif discovery algorithms fix the motif's length as one of the input parameters. In this paper, a novel method is proposed to identify the optimal length of the motif and the optimal motif with that length, through an iteration process on increasing length numbers. For each fixed length, a modified genetic algorithm (GA) is used for finding the optimal motif with that length. Three operators are used in the modified GA: Mutation that is similar to the one used in usual GA but is modified to avoid local optimum in our case, and Addition and Deletion that are proposed by us for the problem. A criterion is given for singling out the optimal length in the increasing motif's lengths. We call this method AMDILM (an algorithm for motif discovery with iteration on lengths of motifs). The experiments on simulated data and real biological data show that AMDILM can accurately identify the optimal motif length. Meanwhile, the optimal motifs discovered by AMDILM are consistent with the real ones and are similar with the motifs obtained by the three well-known methods: Gibbs Sampler, MEME and Weeder.	{ "pile_set_name": "PubMed Abstracts" }
The present and future management of childhood craniopharyngioma. Childhood craniopharyngiomas are rare tumours that present formidable difficulties in their treatment if cure is to be achieved without producing severe hypothalamic damage. Experience with our own cases suggests that the morbidity from an attempted radical removal can be predicted - allowing a treatment algorithm to be devised that combines both surgery (radical and "conservative") and radiotherapy (both external fractionated and intra-cyst instillations) in order to achieve long-term tumour control that is not at the expense of a severe functional disability.	{ "pile_set_name": "PubMed Abstracts" }
Revised chronic widespread pain criteria: development from and integration with fibromyalgia criteria. Background and aims Persons with chronic widespread pain (CWP) have poor medical outcomes and increased mortality. But there are no universally accepted criteria for CWP or of methods to assess it. The most common criteria come from the 1990 American College of Rheumatology (ACR) fibromyalgia (FM) criteria, but that method (WP1990) can identify CWP with as few as three pain sites, and in subjects with wide differences in illness severity. Recently, to correct WP1990 deficiencies, the 2016 fibromyalgia criteria provided a modified CWP definition (WP2016) by dividing the body into five regions of three pain sites each and requiring a minimum of four regions of pain. Although solving the geographic problem of pain distribution, the problem of just how many pain sites (pain diffuseness) are required remained a problem, as WP2016 required as few as four painful sites. To better characterize CWP, we compared four CWP definitions with respect to symmetry, extent of pain sites and association with clinical severity variables. Methods We characterized pain in 40,960 subjects, including pain at 19 individual sites and five pain regions, and calculated the widespread pain index (WPI) and polysymptomatic distress scales (PDS) from epidemiology, primary care and rheumatology databases. We developed and evaluated a new definition for CWP, (WP2019), defined as pain in four or five regions and a pain site score of at least seven of 15 sites. We also tested a definition based on the number of painful sites (WPI ≥ 7). Results In rheumatology patients, WP1990 and WPI ≥ 7 classified patients with <4 regions as WSP. CWP was noted in 51.3% by WP1990, 41.7% by WP2016, 37.6% of WPI ≥ 7 and 33.9% by WP2019. 2016 FM criteria was satisfied in WP1990 (51.1%), WP2016 (63.3%), WPI ≥ 7 (69.0%) and WP2019 (76.6%). WP2019 positive patients had more severe clinical symptoms compared with WP1990, WP2016 and WPI ≥ 7, and similar to but less than FM 2016 positive patients. In stepwise fashion, scores for functional disability, visual analog scale fatigue and pain, WPI, polysymptomatic distress score and Patient Health Questionnaire 15 (PHQ-15) worsened from WP1990 through WP2016, WPI ≥ 7 and WP2019. Conclusions WP2019 combines the high WPI scores of WPI ≥ 7 and the symmetry of WP2016, and is associated with the most abnormal clinical scores. The WP1990 does not appear to be an effective measure. We suggest that CWP can be better defined by combining 4-region pain and a total pain site score ≥7 (WP2019). This definition provides a simple, unambiguous measure that is suitable for clinical and research use as a standalone diagnosis that is integrated with fibromyalgia definitions. Implications Definitions of CWP in research and clinic care are arbitrary and have varied, and different definitions of CWP identify different sets of patients, making a universal interpretation of CWP uncertain. In addition, CWP is a mandatory component of some fibromyalgia criteria. Our study provides quantitative data on the differences between CWP definitions and their criteria, allowing better understanding of research results and a guide to the use of CWP in clinical care.	{ "pile_set_name": "PubMed Abstracts" }
[Prosthetic rehabilitation with dental implant in cleft lip and palate: a ten-year retrospective study]. Two problems need to be faced during cleft lip and palate surgical treatment to gain a functional and esthetic result: restoration of the maxillary bone defect and the missing lateral incisor tooth, which may be replaced by an implant. The aim of our study was to demonstrate that it is possible to successfully place a dental implant after obtaining adequate bone graft volume. 12 consecutively treated patients were studied, 7 with unilateral and 5 with bilateral clefts, mean age 21.5 years, with an average follow-up of 5.5 years (range: 1-10 years). Various types of autogenous or alogenous bone grafts were performed to provide adequate bone volume for immediate or secondary implant placement. We studied the number of bone grafts needed to reach this target, and the number of implants placed, focusing on lateral incisor tooth replacement. Enough cleft bone volume for the implant was obtained in 9 out of 12 cases (75%). All endosseous implants placed in bone graft were successfully restored, 8 out of them for lateral incisor. Some surgical cases are described. Global improving of cleft lip and palate surgical treatment by implantology is now feasible. Maxillary defect bone grafting, often in several stages, is strictly necessary to place an implant but also to obtain a good cosmetic result in cleft repair. Length, direction, and cervical implant position have to be considered too. Reconstruction of mucosal anatomy also contributes to the end result.	{ "pile_set_name": "PubMed Abstracts" }
Liver stiffness-based model for prediction of hepatocellular carcinoma in chronic hepatitis B virus infection: comparison with histological fibrosis. Liver stiffness (LS) value using transient elastography is a reliable, non-invasive tool for assessing liver fibrosis. LS-based prediction model, LSPS (=LS value × spleen diameter/platelet count) is well correlated with the risk of developing portal hypertension-related cirrhotic complications. Here, we assessed the prognostic performance of LSPS in predicting the development of hepatocellular carcinoma (HCC) in patients with chronic hepatitis B (CHB). Between 2006 and 2010, we recruited 227 patients with CHB who underwent liver biopsy and LS measurement. The major end point was HCC development. Median age was 45 years and 156 (68.7%) patients were male. During the follow-up period (median, 61 months), HCC developed in 18 patients. Patient with HCC had a higher LS value, a longer spleen, and lower platelet counts (all P < 0.05) than those without HCC. On multivariate analysis, LSPS was identified as an independent predictor of HCC development [hazard ratio (HR) 1.541, P < 0.001] after adjusting for age, serum albumin level and histological fibrosis stage. When patients were stratified into three groups (LSPS <1.1, 1.1-2.5 and >2.5), the 5-year cumulative risk of HCC increased significantly in association with a higher LSPS value (4.0, 13.8, 36.2%, respectively, P < 0.001). Patients with LSPS 1.1-2.5 (HR 2.0, P = 0.032) and LSPS > 2.5 (HR 8.7, P = 0.002) had a higher risk of developing HCC than those with LSPS < 1.1. LS value-spleen diameter to platelet ratio score is useful for assessing the risk of HCC development and careful surveillance strategies are required in an individual manner.	{ "pile_set_name": "PubMed Abstracts" }
[Nursing care in immunization programs]. The authors answer questions asked by nursing personnel, who work in teaching hospitals and in health centers, about immunization against measles and poliomyelitis, as well as questions about general care in other types of immunization. They recommend specific research on the subject.	{ "pile_set_name": "PubMed Abstracts" }
Anti-oxidized LDL antibodies and wine consumption: a population-based epidemiological study in Dicomano, Italy. In previous studies, higher levels of oxidized low-density lipoprotein (ox-LDL) have been reported to increase the risk of cardiovascular disease. Furthermore, the anti-oxidative properties of red wine have been invoked to explain the paradox of low cardiovascular mortality, in spite of high-fat intake, in the French population. However, the distribution of ox-LDL-antibodies (ox-LDL-Ab) in the unselected population and its relationship with red wine consumption are unknown. This study was carried out to identify factors associated with ox-LDL-Ab, with particular emphasis on the association with wine consumption. We performed a cross-sectional study of 551 unselected, community-dwelling older adults (aged 65 to 94 years) living in Dicomano (Italy). Ox-LDL-Ab titre was measured with an ELISA method. In the whole study sample, ox-LDL-Ab increased with age and was unrelated to blood lipids, HbA(1c), and smoking habit. A significant inverse relationship was found between ox-LDL-Ab titre and daily wine intake, which persisted after adjusting for possible confounders or in subgroup analyses of participants who reported to drink wine or were free from significant morbidity. These results suggest that wine affects ox-LDL-Ab titre in older populations, possibly because of its antioxidant properties.	{ "pile_set_name": "PubMed Abstracts" }
Andrographolide inhibits osteopontin expression and breast tumor growth through down regulation of PI3 kinase/Akt signaling pathway. Breast cancer is one of the most common cancers among women in India and around the world. Despite recent advancement in the treatment of breast cancer, the results of chemotherapy to date remain unsatisfactory, prompting a need to identify natural agents that could target cancer efficiently with least side effects. Andrographolide (Andro) is one such molecule which has been shown to possess inhibitory effect on cancer cell growth. In this study, Andro, a natural diterpenoid lactone isolated from Andrographis paniculata has been shown to inhibit breast cancer cell proliferation, migration and arrest cell cycle at G2/M phase and induces apoptosis through caspase independent pathway. Our experimental evidences suggest that Andro attenuates endothelial cell motility and tumor-endothelial cell interaction. Moreover, Andro suppresses breast tumor growth in orthotopic NOD/SCID mice model. The anti-tumor activity of Andro in both in vitro and in vivo model was correlated with down regulation of PI3 kinase/Akt activation and inhibition of pro-angiogenic molecules such as OPN and VEGF expressions. Collectively, these results demonstrate that Andro may act as an effective anti-tumor and anti-angiogenic agent for the treatment of breast cancer.	{ "pile_set_name": "PubMed Abstracts" }
The face of pathology in Afghanistan in 2006-2007. The current state of the practice of pathology in Afghanistan is described on the basis of visits made by the author to a nongovernmental organization hospital in Kabul, for 6 months between October 2006 and March 2007, and a second visit for 6 weeks at the end of 2007.	{ "pile_set_name": "PubMed Abstracts" }
Studies on short-term application of drugs to Eimeria tenella in tissue culture. The anticoccidial action of 6 drugs was studied by treating cell cultures with drug for 24 h after inoculation of the cultures with sporozoites of Eimeria tenella. A monitoring technique was used to confirm that the drug was being removed. Only monensin was shown to be coccidiocidal. Amprolium, lasalocid, methyl benzoquate, nicarbazin and robenidine were coccidiostatic at their minimum effective doses. Misleading coccidiocidal effects were observed when higher concentrations of methyl benzoquate, nicarbazin or robenidene were used.	{ "pile_set_name": "PubMed Abstracts" }
A feasibility study of induction pemetrexed plus cisplatin followed by extrapleural pneumonectomy and postoperative hemithoracic radiation for malignant pleural mesothelioma. A prospective multi-institutional study has been commenced in Japan to evaluate the feasibility of induction chemotherapy using pemetrexed plus cisplatin, followed by extrapleural pneumonectomy (EPP) and postoperative hemithoracic radiation in patients with resectable malignant pleural mesothelioma. The study was initiated on May 2008 and 40 patients will be recruited over 3 years. Primary endpoints are macroscopic complete resection rate by EPP and treatment-related mortality for trimodality therapy. Secondary endpoints include treatment completion rate, adverse events, response rate by chemotherapy and 2-year overall and relapse-free survival.	{ "pile_set_name": "PubMed Abstracts" }
Effects of 6 Weeks of Balance Training on Chronic Ankle Instability in Athletes: A Randomized Controlled Trial. The objective of the present study was to determine the effectiveness of a 6-week balance training program on patients with Chronic Ankle Instability (CAI) in relation to the results obtained in Dynamic Balance, subjective feeling of instability and pain using a single-blind randomized controlled trial. 70 athletes were randomly assigned to control or intervention group. The control group performed their usual training, and the intervention group was administered the same usual activity in addition to a balance program. The paired t-test was performed to evaluate the change scores in each group. The t-test for independent samples was performed to evaluate between-group differences in change scores. Significance level was assigned for p-values less than 0.05 for all analyses. There were significant differences between groups in change scores in CAIT and all of the SEBTs reach distances (p<0.001) but not in Pain (p=0.586). The effect sizes were larger for the outcomes measures that showed significant differences. In the within-group change, the experimental groups showed larger effect sizes in CAIT, SEBT posteromedial and SEBT posterolateral, and moderate effect sizes in SEBT anterior. Exercise therapy training based on multi-station balance tasks led to significant improvements in dynamic balance and self-reported sensation of instability in patients with CAI.	{ "pile_set_name": "PubMed Abstracts" }
Shear bond strengths of two composite core materials after using all-in-one and single-bottle dentin adhesives. The purpose of this study was to compare the shear bond strengths of 2 composite core materials after using all-in-one and single-bottle dentin bonding materials. The occlusal surfaces of 100 extracted, intact human third molars were ground to expose a flattened area of dentin and polished with 600-grit silicon carbide paper. Specimens were divided into 5 main groups (n= 20). Three all-in-one (AQ Bond, One-Up Bond, Xeno-CF Bond) and 2 single-bottle adhesives (Single Bond, One-Step Plus) were used. Each group was further divided into 2 subgroups. Ti-Core and Built-it F.R. core materials were applied using a translucent plastic ring (diameter: 3 mm, height: 5 mm). After storage in 37 degrees C water for 24 hours, shear bond strengths were measured using a Universal testing machine with a crosshead speed of 0.5 mm/min. Debonded dentin surfaces were examined with SEM. Two-way analysis of variance (ANOVA) and multiple comparison (Tukey) tests were used for statistical analysis of data. Two-way ANOVA revealed that the type of core material did not significantly influence the shear bond strength (p > 0.05), whereas there were significant differences in shear bond strength among the types of bonding agents (p < 0.0001). Shear bond strengths for single-bottle adhesive systems were significantly higher than those for all-in-one adhesive systems (p < 0.05). Furthermore, the interaction of these 2 parameters was not significant (p > 0.05). The fracture modes were predominantly adhesive for all-in-one adhesives and cohesive for single-bottle adhesives. Bonding of composite core materials with the newly developed all-in-one dentin adhesives produced lower shear bond strengths as compared with single-bottle adhesives.	{ "pile_set_name": "PubMed Abstracts" }
Electrophoresis of a pH-regulated zwitterionic nanoparticle in a pH-regulated zwitterionic capillary. We consider the electrophoresis of a rigid sphere along the axis of a narrow cylindrical capillary; both are pH-regulated and zwitterionic. This extends available analyses in the literature to a more general and realistic case. Adopting a titanium oxide (TiO2) particle in a silicon dioxide (SiO2) capillary as an example, we examine the capillary radius, the solution pH, and the electrolyte concentration (or double-layer thickness) for their influences on the electrophoretic behavior of a particle. Because the pH solution is adjusted by HCl and NaOH, the presence of four kinds of ionic species, namely, H(+), OH(-), Na(+), and Cl(-), should be considered if NaCl is the background electrolyte. This also extends conventional electrophoresis analyses to the case of multiple ionic species. The interactions of the electroosmotic flow, the properties of the particle and the solution, and the capillary wall yield complicated electrophoretic behavior that can be regulated by the solution pH and the background electrolyte concentration. The results gathered are necessary for the future design of nanopore-based electrophoresis devices.	{ "pile_set_name": "PubMed Abstracts" }
Comparison of analgesic techniques for antler removal in halothane-anaesthetized red deer (Cervus elaphus): cardiovascular and somatic responses. To compare changes in heart rate and arterial pressures resulting from compression of the antler pedicle or lidocaine 'ring block' and during subsequent antler removal during minimal halothane anaesthesia. Twenty-nine 2-year-old red deer (Cervus elaphus) stags, weighing 106-131 kg and carrying immature growing antler suitable for commercial harvest were studied. Anaesthesia was induced using intravenous propofol (median dose 8.0 mg kg(-1), range 5.2-11.0) and ketamine (median dose 2.2 mg kg(-1), range 1.9-2.4) and maintained using halothane in oxygen. End-tidal halothane concentration (Fe'HAL) end-tidal CO(2) tension (Pe'CO(2)), SpO(2), EEG, ECG, and direct systolic (SAP) mean (MAP) and diastolic (DAP) arterial pressures were recorded continuously. Respiratory rate and somatic responses were recorded. Baseline data were recorded once anaesthesia (Fe'HAL approximately 0.8%) was stable. Stags were randomly allocated to control, lidocaine 'ring block' or compression band treatment groups. One antler was removed 4 minutes after treatment. Cardiovascular responses to the application of analgesia and antler removal were analysed using a general estimates equation for repeated measures or area under the curve (AUC) analysis. Mean AUC was compared between groups using anova, and when significant differences were found, groups were compared post hoc with two-tailed t-tests. Somatic response data were compared with Fisher's exact chi-square test. A value of p < 0.05 was considered significant. Heart rate fell during observations in all groups with no significant differences between groups. Arterial pressures in the control and lidocaine groups during treatment and removal were not different from baseline values or from each other. Compression group pressures were significantly higher than baseline during both treatment and removal. Compression group DAP and MAP were significantly higher after antler removal than during treatment. In control and lidocaine groups, the AUC for SAP, DAP, and MAP over the combined baseline, treatment, and removal period did not differ. The compression group AUC for DAP and MAP were significantly greater over the experimental period than both the lidocaine group and control groups. Somatic responses occurred in one animal at lidocaine injection and three at compression application. Somatic responses occurred in eight control animals and two in the compression group at antler removal. More animals responded to antler removal in the control group than in the compression (p = 0.015) or lidocaine (p < 0.001) groups. Compression of the antler pedicle appears to be noxious. Pedicular compression is a less effective analgesic technique for antler removal compared to 'ring blocks' with lidocaine. This study suggests that lidocaine 'ring blocks' are the current technique of choice for antler removal in deer.	{ "pile_set_name": "PubMed Abstracts" }
Brachyury is a target gene of the Wnt/beta-catenin signaling pathway. To identify target genes of the Wnt/beta-catenin signaling pathway in early mouse embryonic development we have established a co-culture system consisting of NIH3T3 fibroblasts expressing different Wnts as feeder layer cells and embryonic stem (ES) cells expressing a green fluorescent protein (GFP) reporter gene transcriptionally regulated by the TCF/beta-catenin complex. ES cells specifically respond to Wnt signal as monitored by GFP expression. In GFP-positive ES cells we observe expression of Brachyury. Two TCF binding sites located in a 500 bp Brachyury promoter fragment bind the LEF-1/beta-catenin complex and respond specifically to beta-catenin-dependent transactivation. From these results we conclude that Brachyury is a target gene for Wnt/beta-catenin signaling.	{ "pile_set_name": "PubMed Abstracts" }
Correlates of children's compliance with moderate-to-vigorous physical activity recommendations: a multilevel analysis. This study aimed to investigate the association between individual and school characteristics associated with the number of school days children comply with moderate-to-vigorous physical activity (MVPA) recommendations. Sample comprises 612 Portuguese children, aged 9-11 years, from 23 schools. Time spent in MVPA was measured by accelerometry, while individual-level correlates were obtained by anthropometry and questionnaires. School-level variables were collected by questionnaire, and accelerometer wear time and season were also considered. Maximum likelihood estimates of model parameters were obtained via a multilevel analysis with children as level-1, and school as level-2. Children who spent more time in sedentary activities and girls were less likely to comply with MVPA/daily. More mature children and those who use active transportation to school were more likely to attain the PA recommendation. Furthermore, greater accelerometer wear time and spring season increased the chance to achieve the recommended MVPA. In terms of school-level correlates, a greater number of available facilities was negatively associated with children MVPA compliance. Given the set of variables, our results showed that individual characteristics seem to be more relevant for children's compliance rates with PA/day than school context variables, which should be taken into account in the implementation of school policies and practices.	{ "pile_set_name": "PubMed Abstracts" }
Horner Syndrome After Anterior Revision Surgery for Cervical Spondylotic Myelopathy: A Very Rare Complication: A Case Report. We report a case of postoperative left-sided Horner syndrome (blepharoptosis, miosis, and anhidrosis) after revision surgery for cervical spondylotic myelopathy. A C4 corpectomy and an anterior cervical fusion from C3 to C5 were performed through a left longitudinal approach in a patient with a surgical history of an anterior discectomy and a fusion from C5 to C6. One year after the revision surgery, the patient had recovered from the anhidrosis and the miosis, but the blepharoptosis was not fully resolved. Horner syndrome resulting from surgical injury to the ipsilateral cervical sympathetic chain is a very rare complication of anterior spine surgery that has been reported when the lower cervical levels have been approached. Awareness of this important cervical structure may help to avoid this complication.	{ "pile_set_name": "PubMed Abstracts" }
Cancer treatment: what's ahead? Surgery, chemotherapy, and radiation therapy are standard modalities for cancer treatment. Biological therapy (immunotherapy, biotherapy, or biological response modifier therapy) is a comparatively novel addition to this armamentarium. Biological therapies use the body's immune system, either directly or indirectly, to fight cancer or to lessen the side effects that may be caused by some cancer treatments. Biological therapeutic agents include interferons, interleukins, colony-stimulating factors, monoclonal antibodies, vaccines, gene therapy, and nonspecific immunomodulating agents. A promising form of cancer treatment is immunotherapy. Immunotherapy for cancer is essentially the stimulation of the immune system through a variety of reagents such as vaccines, infusion of T-cells, or cytokines. These reagents act through one of several mechanisms including stimulating the anti-tumor response, decreasing suppressor mechanisms, altering tumor cells to increase their immunogenicity and making them more susceptible to immunologic defenses, and improving tolerance to cytotoxic agents or radiotherapy. This review describes some novel approaches in the immunotherapy in cancer.	{ "pile_set_name": "PubMed Abstracts" }
Prospective Validation of Objective Prognostic Score for Advanced Cancer Inpatients in South Korea: A Multicenter Study. Objective Prognostic Score (OPS) was developed as an easy and simple prognosticating tool in South Korea. It has been validated retrospectively in a single center in South Korea. We aimed to validate the OPS prospectively for advanced cancer inpatients in South Korea using a multicenter study. This was a prospective cohort study. We enrolled 243 advanced cancer patients admitted in five palliative care units in South Korea from May 2013 till March 2015. Seven members of the Korean Palliative Medicine Research Network who are experts of palliative care led the study. Clinical variables (dyspnea/anorexia/performance status) and laboratory variables (total leukocyte counts/serum total bilirubin/serum creatinine/lactate dehydrogenase) were collected at the enrollment. Survival time was calculated as days from enrollment to death during admission. A total of 217 patients were included in the final analysis (feasibility: 89.3%). Survival time of the higher OPS group (OPS ≥3) and the lower OPS group (OPS <3) was 10.0 (95% confidence interval (CI) 7.72-12.28) days and 32.0 (95% CI 25.44-38.56) days, respectively. There were significant differences between the 2 groups (p < 0.001). Overall accuracy of OPS ≥3 for predicting survival less than three weeks was 71.0%. OPS was successfully validated using a prospective multicenter study in South Korea. It is a useful method to predict three-week survival of Korean inpatients with advanced cancer.	{ "pile_set_name": "PubMed Abstracts" }
Femoral neck fractures. 121 cases treated by Knowles pinning. The femoral neck fracture remains one of the unsolved fractures. It is a fracture with a high incidence of nonunion and avascular necrosis. One hundred twenty-one mostly young adult patients with femoral neck fractures were treated by Knowles pins internal fixation. Patients were observed for an average of 32 months following surgery. Union occurred in all Garden Stage I and II undisplaced fractures, and the incidence of nonunion was 14% in Garden Stage III and IV displaced fractures. The incidence of avascular necrosis was 5.9% in undisplaced fractures and 34.5% in displaced fractures. There was no significant difference in avascular necrosis rates among age groups. Nonunion and avascular necrosis occurred mainly in displaced fractures. Knowles pinning offers the advantages of few technical failures, early weight bearing, high union rate, and low complication rate.	{ "pile_set_name": "PubMed Abstracts" }
Spirometric reference values in a large Middle Eastern population. Ethnic differences in pulmonary function have been frequently reported. The purposes of this study were to derive equations for the prediction of normative spirometry values for a large population of Persians in Isfahan and compare them to reference values from a White Euro-USA population. Spirometry measurements were obtained from 4,341 randomly selected healthy nonsmoker subjects in Isfahan, Iran, utilising American Thoracic Society guidelines and a vigorous quality assurance program. Measured data from 3,213 subjects were analysed using multiple regression techniques to derive prediction equations for spirometric variables; the remaining 1,128 subjects were used as a control group to test the validity of the derived equations. In addition, predicted values were compared with values derived from recently published equations for the USA. Derived prediction equations showed good performance for most spirometric parameters. Compared with USA Whites, adult Persians have minimally lower forced vital capacities, while the values for children are close to USA Whites. In comparison with reference equations based on European or USA populations, local reference values are more biologically and technically suitable for the interpretation of spirometric data from Iranian populations.	{ "pile_set_name": "PubMed Abstracts" }
Adalimumab for Induction of Histological Remission in Moderately to Severely Active Ulcerative Colitis. Histological remission represents a target distinct from endoscopic healing in ulcerative colitis (UC) and seems a better predictor of clinical outcomes. The aim of this study was to assess the ability of adalimumab to achieve histological remission in UC patients. Single-center, retrospective, open-label study of patients treated with adalimumab. Eligible patients were anti-TNF naïve adults with moderately to severely active UC. The Mayo score including endoscopy was performed at baseline and weeks 8 and 52. Histological activity was scored using the Geboes Index. The primary endpoint was histological remission, defined as a Geboes grade ≤ 3.0, at week 52. We included 34 patients. At week 8, 6 of 34 patients (17.6%) achieved histological remission. At week 52, 9 patients (26.5%, intention to treat; 31%, per protocol) had histological remission. Patients had a significant and progressive reduction in the most severe subgrades of Geboes Index from baseline at weeks 8 and 52. At weeks 8 and 52, 50 and 61.8% of patients achieved mucosal healing (Mayo endoscopic subscore 0-1). All patients who achieved histological remission also had mucosal healing. At week 8, 85.3 and 20.6% of patients achieved clinical response (decrease in Mayo score ≤ 3 points) or remission (Mayo score ≤ 2), respectively. At week 52, the corresponding values were 67.6 and 52.9%, respectively. At week 52, agreement between histological remission and mucosal healing was fair (kappa 0.293). Agreement between histological remission and Mayo endoscopic subscore 0 was good (kappa 0.71). Adalimumab was able to achieve histological remission in anti-TNF naïve patients with moderately to severely active UC.	{ "pile_set_name": "PubMed Abstracts" }
Controlled cellular orientation on PLGA microfibers with defined diameters. In this study, we investigated the effects of the diameter of microfibers on the orientation (angle between cells' major axis and the substrate fiber long axis) of adhered cells. For this purpose, mouse fibroblast L929 cells were cultured on the surface of PLGA fibers of defined diameters ranging from 10 to 242 mum, and their adhesion and alignment was quantitatively analyzed. It was found that the mean orientation of cells and the spatial variation of cell alignment angle directly related to the microfiber diameter. Cells that were cultured on microfibrous scaffolds oriented along the long axis of the microfiber and the orientation increased as the fiber diameter decreased. For the fiber diameter of 10 microm, the mean orientation was 3.0 +/- 0.2 degrees (mean +/- SE), whereas for a diameter of 242 microm, it decreased to 37.7 +/- 2.1 degrees . Using these studies we demonstrate that fibroblasts have a characteristic alignment on microscale fibers and that the microscale fiber diameter plays a critical role in cellular orientation. The ability to control cellular alignment on engineered tissue scaffold can be a potentially powerful approach to recreate the microscale architecture of engineered tissues. This may be important for engineering a variety of human tissues such as tendon, muscle and nerves as well as applications in 3D tissue culture and drug screening.	{ "pile_set_name": "PubMed Abstracts" }
Suppression of NF-kappaB-dependent gene expression by a hexamethylene bisacetamide-inducible protein HEXIM1 in human vascular smooth muscle cells. Neointima formation is a characteristic feature of atherosclerosis and post-angioplasty restenosis, in which various soluble factors and mechanical injury stimulate signalling pathways in vascular smooth muscle cells (VSMC), promoting their migration and proliferation, and the eventual formation of the neointima. The transcription factor NF-kappaB has been shown to play a pivotal role in this process. Hexamethylene bisacetamide, an inhibitor of VSMC proliferation, induces the mRNA expression of HEXIM1 (hexamethylene bisacetamide-inducible protein 1). However, the protein expression and function of HEXIM1 remain unknown. In the present study, we demonstrated that HEXIM1 localizes in the cytoplasm and nucleus, and its nuclear expression is restricted to discrete speckled areas. Treatment of VSMC with hexamethylene bisacetamide up-regulated HEXIM1 expression, not only in mRNA but also protein levels. Moreover, HEXIM1 is shown to suppress the transcriptional activity of NF-kappaB via its C-terminal leucine-rich domain. A glutathione-S-transferase pull down assay indicated that HEXIM1 interacts with the p65 subunit of NF-kappaB. In VSMC, treatment with hexamethylene bisacetamide resulted in a down-modulation of the transcription of NF-kappaB target genes. We may therefore conclude that HEXIM1 plays an inhibitory role in NF-kappaB-dependent gene expression in VSMC and is the candidate of a novel therapeutic target for inhibition of VSMC proliferation.	{ "pile_set_name": "PubMed Abstracts" }
The autophagic machinery is necessary for removal of cell corpses from the developing retinal neuroepithelium. Autophagy is a homoeostatic process necessary for the clearance of damaged or superfluous proteins and organelles. The recycling of intracellular constituents also provides energy during periods of metabolic stress, thereby contributing to cell viability. In addition, disruption of autophagic machinery interferes with embryonic development in several species, although the underlying cellular processes affected remain unclear. Here, we investigate the role of autophagy during the early stages of chick retina development, when the retinal neuroepithelium proliferates and starts to generate the first neurons, the retinal ganglion cells. These two developmental processes are accompanied by programmed cell death. Upon treatment with the autophagic inhibitor 3-methyladenine, retinas accumulated numerous TdT-mediated dUTP nick-end labelling-positive cells that correlated with a lack of the 'eat-me' signal phosphatidylserine (PS). In consequence, neighbouring cells did not engulf apoptotic bodies and they persisted as individual cell corpses, a phenotype that was also observed after blockade of phagocytosis with phospho-L-Serine. Supplying the retinas with methylpyruvate, a cell-permeable substrate for ATP production, restored ATP levels and the presentation of PS at the cell surface. Hence, engulfment and lysosomal degradation of apoptotic bodies were also re-established. Together, these data point to a novel role for the autophagic machinery during the development of the central nervous system.	{ "pile_set_name": "PubMed Abstracts" }
[Model of intraluminal perfusion of the guinea pig ileum in vitro in the study of the antidiarrheal properties of the guava (Psidium guajava)]. An experimental in vitro model was developed for the study of plant extracts reported by traditional medicines in the treatment of diarrhea. The guinea-pig isolated ileum is perfused with the plant extract using an intraluminal approach. The peristaltic reflex is induced by electrical stimulation while the plant extract is perfused. The spasmolytic effects of Psidium guajava leaf methanol, hexane and water extracts were demonstrated suggesting the existence of two different types of active components. The results obtained allow to propose this in vitro method as a useful model to reproduce some of the characteristics of the oral way of administration of plant extracts.	{ "pile_set_name": "PubMed Abstracts" }
Influence of ageing, pH and various additives on crystal formation in artificial urine. In order to investigate the effect of various factors on urinary crystallization processes, a series of five experiments was carried out using an artificial urine (AU) in a rotary evaporator. The influence of ageing, pH and organic, inorganic and potential inhibitory additives formed the basis of the study. Precipitates were characterized by X-ray powder diffraction, scanning electron microscopy and energy dispersive X-ray analysis. In the ageing experiment, AU aliquots, adjusted to various pH values, were allowed to stand for several days and were not evaporated. Calcium oxalate monohydrate (COM) was formed at low pH, while whitlockite, apatite and struvite occurred at pH greater than 7. In the second experiment, AU aliquots at various pH values, were evaporated. Similar results to those of series 1 were recorded but, in addition, calcium oxalate trihydrate (COT) precipitated in the pH range 3 to 6.5 and brushite at pH greater than 5.5. In series 3, uric acid, creatinine and urea were included in AU aliquots (pH 5.5) which were subjected to evaporation. Uric acid promoted the formation of uric acid dihydrate; however, when present with creatinine, dihydrate formation was inhibited. Urea appeared to inhibit precipitation. In the fourth experiment, MgO, methylene blue and chondroitin sulphate A were independently included in the AU (pH 5.5). Precipitates of calcium oxalate mono-, di- and trihydrates were obtained. In the final experiment fluoride aliquots of variable concentrations were included in the AU (pH 5.5 and 6.5). COT crystals of superior quality to those observed in control solutions were obtained.	{ "pile_set_name": "PubMed Abstracts" }
The effect of 2-oxoglutarate and biotin in the release of amino acids by Citrobacter intermedius C3. Excretion of amino acids by Citrobacter intermedius C3 was assayed in a mineral medium with glucose as carbon source. Glutamic acid is the main amino acid excreted in liquid medium and it is also detected at the colonial level in solid medium. Mutants with different behaviour with respect to the excretion of amino acids are studied. The presence of 2-oxoglutarate in the medium induced excretion in all strains. On the other hand when biotin was added to the culture media amino acid excretion was partially reduced.	{ "pile_set_name": "PubMed Abstracts" }
[Concentration and distribution of insulin and insulin-like protein in the organs of normal and diabetic mice]. Presence of insulin or insulin-like protein has been studied in mouse liver, kidneys, lungs, duodenum, jejunum, submandibular and parotid salivary glands, in femoral, diaphragmal and abdominal wall muscles by means of the immunofluorescent method. In order to understand the role of the extrapancreatic insulin for compensation of the insular insufficiency, corresponding organs have been examined in mice with alloxan diabetes. The immunoreactive insulin is proved to be present only in cells of the granular parts of the salivary tubules of the submandibular and striated ducts of the parotid glands. As demonstrates microfluorometry, a relative amount of insulin in the submandibular gland cells is 1.5 times and in the parotid gland cells--2 times as small as in beta-cells of the pancreatic glands. Under alloxan diabetes insulin content in the salivary gland cells decreases by 1.3-1.9 times (in the beta-cells--by 2.7 times). This may designate that the extrapancreatic insulin (or insulin-like protein) participates in compensation of hypoinsulinemia. In mice with alloxan diabetes, immunoreactivity of insulin is also revealed in hepatocytes.	{ "pile_set_name": "PubMed Abstracts" }
The differential effects of external ankle support on postural control. Ankle supports are commonly used in an attempt to decrease the risk of ankle injury during sport. However, their use may also impair postural control, which is an integral component of sports participation. The aim of this study was to investigate the effects of three different ankle supports (tape, brace, and elastic bandage) on postural control in 24 normal subjects with a mean age of 24.8 years (+/- 4.4). Two measures were used to evaluate postural control in one-legged stance with the eyes closed: variability of mediolateral ground reaction force (acquired from a force platform) and frequency of foot touchdowns by the nonsupport leg (assumed to indicate ability of the subject to maintain one-legged stance posture). Both measures revealed a differential effect for ankle support on postural control. The use of an elastic bandage had no significant effect on postural control (p > 0.05), while the use of tape or a brace had a significant detrimental effect (p < 0.05). While wearing the tape or a brace, subjects were less steady and touched down more frequently. Restriction of ankle movement was offered as a possible explanation for the results, since postural control was impaired only by the ankle supports which limited ankle motion. These findings may have implications regarding impaired athletic performance.	{ "pile_set_name": "PubMed Abstracts" }
TLR4 signaling is involved in the protective effect of propofol in BV2 microglia against OGD/reoxygenation. Propofol exhibits neuroprotective effects against hypoxic-ischemic brain injury, but the underlying mechanisms are still not clear. Toll-like receptor 4 (TLR4) plays a considerable role in the induction of innate immune and inflammatory responses. The purposes of this study are to investigate the effect of propofol on the oxygen and glucose deprivation (OGD)/reoxygenation (OGD/R) BV2 microglia and to explore the role of TLR4/myeloid differentiation protein 88 (MyD88)/nuclear factor-kappa B (NF-κB) pathway in the neuroprotective effects of propofol. BV2 microglia were placed into an airtight chamber and in glucose-free medium for OGD/reoxygenation. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay. TLR4 and its downstream signaling molecules, MyD88 and NF-κB expressions were detected by Western blotting. Level of tumor necrosis factor alpha (TNF-α) in culture medium was determined with enzyme-linked immunosorbent assay. BV2 microglia apoptosis was determined by flow cytometry. We found that pretreatment with propofol significantly alleviated the hypoxic injury in BV2 microglia. Propofol inhibited upregulation of TLR4, MyD88, and NF-κB expressions in BV2 microglia exposed to OGD/reoxygenation. Propofol pretreatment also significantly reduced the production of TNF-α and apoptosis in OGD/reoxygenation BV2 microglia. The results indicated that TLR4 and its downstream MyD88-dependent signaling pathway contributed to neuroprotection of propofol to microglia exposed to OGD/reoxygenation.	{ "pile_set_name": "PubMed Abstracts" }
Plate fixation versus conservative treatment of displaced midshaft clavicle fractures: Functional outcome and patients' satisfaction during a mean follow-up of 5 years. The aim of the present prospective clinical trial was to compare patient-oriented and surgeon-based outcomes after non-operative care with operative treatment of displaced midshaft clavicle fractures. Between January 2009 and July 2011, 97 consecutive patients presenting with a midshaft clavicle fracture were prospectively recorded and included in this study. The patients were placed in either of the treatment groups on their own preference. They were then seen in outpatient clinic at two, six and 24 weeks were all endpoints were investigated and motivation of choice of treatment was noted. Study follow-up was continued until Augustus 2014, being the time point that long-term functional outcome was measured through a DASH score by letter. 97 patients were included in the functional outcome analysis. The mean DASH and Constant scores were significant better in the operative (90.9±14.2 and 15.7±17.2) than in the conservative treatment group at six weeks (78.7±17.0 and 24.8±16.7). There was a significant improvement in the Constant (95.9±10.5 versus 94.5±5.9) and DASH scores (8.8±12.0 versus 7.1±10.7) for both groups at 24 weeks but there was no significant difference in functional scores between the groups. Four patients developed a non-union, one patient in the operative and three patients in the conservative group. Overall complications were significantly higher in the operative group (31%) compared to the conservative group (9%) (p<0.001). There was no significant difference in long-term functional outcome between the two treatment groups (5.2±9.8 versus 2.5±4.9 p=0.12). Patient's satisfaction was higher in the operative than in the conservative group (p<0.04). Significant superior outcome scores were seen at six weeks for the operative group. However, at 24 weeks and 5-year follow-up no difference was seen in functional outcome scores for both treatment groups. Therefore, the challenge for the future is to better identify the subgroup of patients who might benefit from primary surgical intervention.	{ "pile_set_name": "PubMed Abstracts" }
Questionable history of immediate-type hypersensitivity to penicillin in Staphylococcal endocarditis: treatment based on skin-test results vers-us empirical alternative treatment--A decision analysis. Approximately 10% of the population claim to be allergic to penicillins, but only approximately 10%-30% of these have IgE-mediated reactions to penicillin skin tests. Alternatives to penicillins may be less effective, more toxic, and more expensive. Therefore, we used decision analysis to calculate maximum expected utility and minimum cost for skin-testing or not skin-testing patients who have endocarditis due to Staphylococcus aureus that is susceptible to cloxacillin and who have a questionable history of immediate-type hypersensitivity to penicillin. We used known probabilities of intermediate outcomes, actual costs, and measured utilities and included one-way sensitivity analysis. Whether utility, cost, or average cost-utility was the outcome of interest, skin-testing was preferred to no skin-testing in most conditions. Patients who have endocarditis due to S. aureus that is susceptible to cloxacillin and who also have a questionable history of immediate-type hypersensitivity to penicillin should be skin-tested before starting antibiotic therapy.	{ "pile_set_name": "PubMed Abstracts" }
D1- and D2-like receptors in the dentate gyrus region of the hippocampus are involved in the reinstatement induced by a subthreshold dose of morphine and forced swim stress in extinguished morphine-CPP in rats. Addiction to opioids is an important global problem. Published research has indicated the powerful rewarding effects of drug use, which in the case of opiates like morphine may lead to drug addiction and maladaptive decision making with negative social consequences. In-depth comprehension of the role of responsible mechanisms in addiction can lead us to better and more effective treatments for drug dependence. Continuing previous work in our laboratory, in this study we aimed to investigate the role of dopamine D1- and D2-like receptors in the dentate gyrus (DG) on the reinstatement of drug-seeking behavior induced by the combination of forced swim stress and a subthreshold dose of morphine on extinguished morphine-conditioned place preference in rats. The rats were bilaterally implanted with 2 separate cannulas into the DG region. After the extinction phase of morphine-conditioned place preference, the animals received different doses (0.5, 2, and 4 μg per 0.5 μL vehicle/side) of SCH-23390 or sulpiride on the reinstatement day and were tested for the combination of forced swim stress and a subthreshold dose of morphine in discrete groups. Our findings indicated that D1- and D2-like receptor antagonists attenuated the reinstatement induced by the combination of FSS and the subthreshold dose of morphine. The reduction was more robust in groups of animals that received sulpiride as compared with SCH-23390. Our results showed a role for DG dopamine receptors in relapse to drugs of abuse, the activity of which may be induced by exposure to a stressor like forced swim stress. (PsycINFO Database Record (c) 2019 APA, all rights reserved).	{ "pile_set_name": "PubMed Abstracts" }
Haloperidol-stomach lesions attenuation by pentadecapeptide BPC 157, omeprazole, bromocriptine, but not atropine, lansoprazole, pantoprazole, ranitidine, cimetidine and misoprostol in mice. The focus was on haloperidol (central dopamine antagonist)-stomach lesion, a longly described suitable counterpart of dopamine blocker cysteamine-duodenal lesion. In this, the contribution of blockade of central/peripheral dopamine receptors and prostaglandins synthesis, along with influence of antiulcer agents was evaluated in mice. Male NMRI Hannnover mice were sacrificed 24 h after haloperidol (25 mg/kg b.w. i.p., given alone or with saline (haloperidol+saline) (i) or in combination (ii,iii)). Supporting central dopamine predominance for haloperidol stomach lesion induction, co-administration of peripheral dopamine receptor antagonist domperidone (5 mg/kg i.p.) (haloperidol+ domperidone) (ii), or prostaglandin synthesis inhibitor indomethacin (10 mg/kg s.c.) (haloperidol+ indomethacin) (iii) did not aggravate this lesion. (i) In haloperidol+saline challenged mice the lesions were inhibited by co-administration (/kg i.p.) of a gastric pentadecapeptide BPC 157, GlyGluProProProGlyLysProAlaAspAspAlaGlyLeuVal, M.W. 1419 (10 microg, 10 ng, 10 pg, but not 1 pg, 100 fg, 10 fg), bromocriptine (10 mg), omeprazole (10 mg, 100 mg, but not 1 mg). Atropine (10, 100, 200 mg), pirenzepine (10, 100, 200 mg), misoprostol (10, 100, 200 microg), pantoprazole (1, 10, 100 mg), lansoprazole (0.1, 1, 10 mg), cimetidine (10, 100, 200 mg) and ranitidine (10, 100, 200 mg) were not effective. (ii) Dopamine peripheral blockade influence: in haloperidol+domperidone mice, previously effective bromocriptine, pentadecapeptide BPC 157 (10 microg) or omeprazole (10 mg) did not attenuate stomach lesions. (iii) Prostaglandins synthesis blockade effect: in haloperidol+indomethacin mice, previously effective agents, bromocriptine or omeprazole were not active, while BPC 157 effect was only lessened.	{ "pile_set_name": "PubMed Abstracts" }
Effects of treatment with growth hormone and somatostatin on efficacy of diammine [1,1-cyclobutane dicarboxylato (2-)-0,0']-(SP-4-2) in athymic rats with osteosarcoma. To determine the effect of exogenous growth hormone or somatostatin on chemotherapeutic efficacy in athymic (nude) rats with osteosarcoma. 66 female athymic rats. Osteosarcoma was induced at an intratibial site. Rats were randomly allotted to 6 treatment groups. Rats were treated with saline (0.9% NaCl) solution alone, platinum, diammine [1,1-cyclobutane dicaboxylato (2-)-0,0']-(SP-4-2) (CBDCA; ie, carboplatin) plus saline solution, somatostatin alone, somatostatin plus CBDCA, growth hormone alone, or growth hormone plus CBDCA. Variables measured included estimated WBC count and percentage of neutrophils, plasma concentration of insulin-like growth factor I (IGF-I), body weight, tumor volume, weight of primary tumor, survival time, and distant metastasis at time of death. Tumors formed at the injection sites in all rats. Treatment with growth hormone increased, and treatment with somatostatin decreased, plasma IGF-I concentration. Treatment with growth hormone or somatostatin altered CBDCA efficacy, as determined by evaluation of mean and median survival times. Metastatic pulmonary disease developed in 63 of 64 rats. The technique used here reliably induced local osteosarcomas and metastatic pulmonary disease. Treatment with growth hormone and CBDCA or somatostatin may improve chemotherapeutic efficacy without increasing toxic effects. IMPLICATIONS FOR HUMAN MEDICINE: Results reported here may be useful in the study of osteosarcoma in humans.	{ "pile_set_name": "PubMed Abstracts" }
Studies on the compartmentation of lipid in adipose cells. I. Subcellular distribution, composition, and transport of newly synthesized lipid: liposomes. The subcellular distribution and composition of endogenously synthesized lipid in isolated white adipose cells were studied to determine the nature and extent of lipid compartmentation. After brief incubation of cells with labeled glucose, acetate, or palmitic acid, over 90% of newly synthesized triglyceride was localized in the bulk-lipid phase, indicating rapid intracellular transport and storage. From 13 to 20% of the newly formed lipid was diglyceride, and over 95% of it was localized in the central lipid-storage vacuole rather than in organelle systems concerned with esterification, thus indicating intracellular segregation of newly synthesized partial glycerides. Most of the newly synthesized phosphatides partitioned with membranous organelles. Synthesis of cholesterol or cholesteryl ester was negligible. After brief incubation of cells with labeled glucose, the relative specific activity of organelle triglyceride was mitochondria >> microsomes > liposomes > soluble supernatant > bulk lipid. In pulse-chase studies the specific activity of organelle triglyceride decreased and that of the bulk fraction increased reflecting intracellular lipid transport. The data suggest that a significant proportion of newly formed lipid is transferred from mitochondrial membranes into the storage vacuole by direct lipid-lipid interaction. Liposomes, which consist of small enclosed lipid droplets resembling chylomicrons, contained triglycerides of specific activity similar to microsomal triglyceride. While the evidence that liposome triglyceride may be microsomal in origin is indirect, the results do indicate that the liposome fraction represents a phase in the transport and(or) storage of new glyceride. At least two forms of compartmentation of newly synthesized lipids occurred. The first, termed "structural," refers to localization of lipids to organelle fractions. The second type of compartmentation, termed "chemical," concerns the intracellular segregation of a specific lipid class. The accumulation and segregation of newly synthesized diglyceride in the bulk storage pool are examples of the latter form of compartmentation.	{ "pile_set_name": "PubMed Abstracts" }
Graphical Product Quality and Muscle Activity in Children With Mild Disabilities Drawing on a Horizontally or Vertically Oriented Tablet. We compared performance level and muscle activity patterns during shape copying and tracing in two positions, while sitting at a desk and while standing in front of a wall, between typically developing (TD) preschool children and children with mild disabilities (MD). Twenty-two TD children (8 boys, 14 girls; mean [M] age = 5.2 yr, standard deviation [SD] = 0.1) and 13 children with MD (9 boys, 4 girls; M age = 4.9 yr, SD = 0.5) participated in this study. The children performed faster and smoother movements when copying shapes on the vertical surface, with no reduction of accuracy, than on the horizontal surface. Children with MD exerted their upper trapezius while performing the short tasks on the vertical surface compared with their muscle activity on the horizontal surface. Incorporating short copying or drawing tasks on a vertical surface may increase the control of proximal muscles and ease graphomotor performance in children with MD.	{ "pile_set_name": "PubMed Abstracts" }
The use of reverse diffusion to validate the performance of diffusive samplers. A number of different protocols have been put forth for measuring reverse diffusion from diffusive samplers. The basic concept is that reverse diffusion tests, depending as they do on basic laws of mass transfer, are not independent of one another, but may give general information about the limits to the possible change that can occur if the conditions to measure reverse diffusion are changed. Laboratory measurements of the reverse diffusion of vinyl chloride, using 3M and SKC diffusive samplers, following both the National Institute for Occupational Safety and Health (NIOSH) and the European Union test protocols, support the mathematical analysis developed in this article. An important conclusion is that if in following the NIOSH protocol a diffusive sampler loses 10% of its analyte over a period of 4 hours, then the maximum loss expected from a sampler allowed to back-diffuse for 8 hours is 19%.	{ "pile_set_name": "PubMed Abstracts" }
An ambispective observational study in the safety and efficacy of posterior colporrhaphy with composite Vicryl-Prolene mesh. There is increasing evidence to show that the use of surgical meshes reduces recurrence rates of hernia repair and anterior vaginal wall prolapse. The aim of this study was to determine the safety and efficacy of posterior colporrhaphy with mesh in patients with posterior vaginal prolapse. An ambispective observational study involving 90 patients was conducted with retrospective chart review and prospective subjective and objective assessments at the end of a 1-year study period. Apart from 2 of 90 (2.2%) minor hematoma incidents, there was no other major perioperative morbidity. Prevalence of common prolapse complaints of vaginal lump sensation, constipation, defecation difficulty and dyspareunia all improved significantly postoperatively (p<0.001). Surgical correction was achieved in 27 of 31 (83.9%) at 6 months and beyond. There was no mesh infection but minor vaginal mesh protrusion was found in 7 of 90 (7.8%) patients at 6-12 weeks and 4 of 31 (12.9%) patients at 6 months and beyond. All these were treated easily with trimming without the need of mesh removal. We conclude that posterior colporrhaphy with mesh is effective in treating posterior vaginal prolapse in short term.	{ "pile_set_name": "PubMed Abstracts" }
[Sanitary specification by electromagnetic factor for radiofrequency transmitters]. The article represents structure and contents of sanitary specification by electromagnetic factor to unify radio transmitter. The authors determined requirements to calculate intensity of radio frequency electromagnetic rays and to complete calculations in sanitary specification. Complete information on calculation of radio frequency electromagnetic rays intensity should be stored in electronic carrier (diskette). Notion is that using software complex analyzing electromagnetic situation could resolve many problems and discrepancies in coordination of sanitary specification by State Sanitary and Epidemiologic Supervision.	{ "pile_set_name": "PubMed Abstracts" }
[Preventive effect of gubao on hydrocortisone-induced osteoporosis in rats]. The antagonistic effect of Gubao (consisting of Herba Epimedii, Radix Astragali, etc.) on hydrocortisone-induced osteoporosis in rats was observed in quantitative bone histomorphometry study with stereological method. The results of experiment show that Gubao helps to reduce trbecular bone resorption and increase bone formation in ratsuith hydrocortisone-induced osteoporosis. It is suggested that Gubao is useful in preventing glucocorticoid-induced osteoporosis.	{ "pile_set_name": "PubMed Abstracts" }
Brain-stem monitoring. II. Preterminal BAEP changes observed until brain death in deeply comatose patients. Preterminal BAEP changes were studied until brain death in 8 head-injured patients out of a series of 38 comas monitored by means of a system allowing high-rate sequential recording. Two different modalities of BAEP degradation were disclosed: (1) simultaneous latency increase of all components associated with a decrease of cerebral perfusion pressure (CPP), consistent with ongoing ischaemia of the posterior fossa; (2) deterioration of brain-stem components (waves III-V) with preserved or even enhanced wave I. The latter pattern was not consistently associated with any haemodynamic change and might be related to mechanical factors causing rostro-caudal deterioration of brain-stem function. The time course of BAEP degradation ranged from a few minutes to more than 10 h. In the case of slow preterminal evolution definitely pathological trends were identified even when individual BAEPs were still within normal limits. Such trends would have remained unnoticed in single BAEP records. Hypothermia and anaesthetic drugs were found to induce falsely alarming BAEP changes very similar to those seen during preterminal evolution. Our results suggest that continuous brain-stem monitoring could be helpful for management of comatose head-injured patients.	{ "pile_set_name": "PubMed Abstracts" }
Sidedness of the ATP-Na+-K+ interactions with the Na+ pump in squid axons. Using dialysed squid axons we have been able to control internal and external ionic compositions under conditions in which most of the Na+ efflux goes through the Na+ pump. We found that (i) internal K+ had a strong inhibitory effect on Na+ efflux; this effect was antagonized by ATP, with low affinity, and by internal Na+, (ii) a reduction in ATP levels from 3 mM to 50 microM greatly increased the apparent affinity for external K+, but reduced its effectiveness compared with other monovalent cations, as an activator of Na+ efflux, and (iii) the relative effectiveness of different K+ congeners as external activator of the Na+ efflux, though affected by the ATP concentration, was not affected by the Na+/K+ ratio inside the cells. These results are consistent with the idea that the same conformation of the (Na+ + K+)-ATPase can be reached by interaction with external K+ after phosphorylation and with internal K+ before rephosphorylation. They also stress a nonphosphorylating regulatory role of ATP.	{ "pile_set_name": "PubMed Abstracts" }
Periodontitis in older Swedish individuals fails to predict mortality. This study aims to assess mortality risk and its association to health aspects in dentate individuals 60 years of age and older. Medical and periodontal data from 870 dentate individuals (age range 60–96) participating in the Swedish National Study on Aging and Care in Blekinge (SNACBlekinge)with survival statistics over 6 years were studied. During 6 years of follow-up, 42/474 of the individuals(8.9 %), who at baseline were between age 60 and 75, and 134/396 individuals of the individuals (33.9 %), who at baseline were ≥75 years, died. Surviving dentate individuals had more teeth (mean 19.3, S.D.±7.9) than those who died (mean 15.9,S.D.±7.3; mean diff 3,3; S.E. mean diff 0.7; 95 % CI 2.0, 4.6;p=0.001). A self-reported history of high blood pressure (F=15.0, p<0.001), heart failure (F=24.5, p<0.001, observed power=0.99), older age (F=34.7, p<0.001), male gender(F=6.3, p<0.01), serum HbA1c with 6.5 % as cutoff level(F=9.3, p=0.002) were factors associated with mortality. A medical diagnosis of heart disease, diabetes, any form of cancer,or periodontitis failed to predict mortality. A self-reported history of angina pectoris, chronic heart failure, elevated serum HbA1c, and few remaining teeth were associated with mortality risk. A professional diagnosis of cardiovascular disease, diabetes, cancer, or periodontitis was not predictive of mortality. Self-health reports are important to observe in the assessment of disease and survival in older individual.	{ "pile_set_name": "PubMed Abstracts" }
Correlation of endoscopy and double-contrast radiography in the early stages of ulcerative and granulomatous colitis. Early radiological changes in ulcerative colitis include (a) finely granular mucosa due to hyperemia and edema, (b) mucosal stippling due to adherence of barium to superficial ulcers, and (c) coarsely granular mucosa due to ingrowth of granulation tissue. In granulomatous colitis, early changes include discrete, superficial ulcers, often against a background of normal mucosa, seen best en face. All of these changes can be demonstrated by the double-contrast technique. The radiological and endoscopic findings were in agreement in 95% of the examinations in the authors' series. The importance of the technical aspects of the examination is stressed.	{ "pile_set_name": "PubMed Abstracts" }
[Urinary bilharziasis in Spain (a case in León)]. The authors draw attention to the potential danger of urinary schistosomiasis in Spain, pointing out the nuclei in the country which present snails suitable for being intermediary carriers. They present one case, not autochthonous, observed in León and they point out the possible increase in this parasitosis, due to the increase in immigration and tourism, with the possible infesting of the aboriginal snails and the resulting formation of endemic areas in the said nuclei. In view of the precarious Spanish, urological literature on the subject, a study is made of the illness and the parasitic cycle.	{ "pile_set_name": "PubMed Abstracts" }
Synthesis and biological activities of nucleoside-estradiol conjugates. Nucleosides were coupled to estradiol via a 17alpha-ethynyl spacer group using Pd(II) as a catalyst. The conjugates were evaluated in vitro for estrogen receptor (ER) binding affinity and cytotoxicity against cell lines with and without ER. The highest receptor binding affinities (RBA approximately 3) were observed with conjugates coupled via a relative long spacer group, while none of the conjugates exhibited cytotoxicity against either cell lines.	{ "pile_set_name": "PubMed Abstracts" }
The fatal poisoning pattern of Ankara (Turkey) and nearby cities from 2007 to June 2011: a retrospective study in forensic autopsies. We aimed to obtain an outline of the nature and number of fatal poisonings which still appear to affect widely the population in Turkey. A total of 5921 forensic autopsies were performed between 2007 and 2011 in Ankara and nearby cities and 366 of them were fatal poisonings (219 male, 147 female). Most of the cases were between 41 and 60 years old (n = 84). Most of the fatalities were reported during winter months (48.1%). Carbon monoxide exposure was the most common reason (66.7%) within all the causes followed by medicine and narcotics (13.9%). Postmortem blood alcohol concentrations in ethyl and methyl alcohol poisonings were 385.1 ± 61.9 and 206.8 ± 138.9 mg/dL, respectively. The most common location of deaths was home (71.3%). Fatal poisonings have been a growing global problem because of some shortcomings about the socioeconomic conditions and increasing illicit drug abuse. The level of education, socioeconomic conditions, and legal approaches are very important for the prevention fatal poisonings.	{ "pile_set_name": "PubMed Abstracts" }
Severe congenital stenosis of the left coronary artery ostium and its possible pathogenesis according to current knowledge on coronary artery development. We report a 72-year-old woman with severe congenital stenosis of the left coronary artery orifice and clinically significant atherosclerotic changes in both the right and left coronary arteries. The stenotic ostium was located at the point at which the left and posterior aortic valve leaflets joined to form the left commissure, just at the distal vertex of the left interleaflet triangle, between the left and posterior aortic sinuses. The right coronary artery was more developed in size than usual, whereas the left coronary artery consisted of a short left main coronary trunk that bifurcated into left anterior descending and left circumflex arteries. The left coronary artery system was filled retrogradely through two vessels proceeding from the right coronary artery, namely, the conal artery and a well-developed branch that ran across the interventricular septum. This abnormal arrangement of the coronary arteries showed striking functional similarities with atresia of the left main coronary artery. Current knowledge on the morphogenesis of the coronary arteries suggests that the present anomalous coronary artery pattern resulted from the penetration of the anticipated left coronary artery system into the aorta at a totally erroneus site. This hindered the normal development of the ostium, which subsisted as a punctiform, practically nonfunctional opening.	{ "pile_set_name": "PubMed Abstracts" }
Management of the adnexal mass. The methods for preoperative diagnosis and evaluation of the woman with a suspected ovarian neoplasm have evolved significantly with little or no impact on the surgical treatment, which remains removal of the abnormal ovary. Although most adnexal masses are benign, the primary goal of the diagnostic evaluation is the exclusion of malignancy. A complete history, physical examination, and ultrasonic imaging are vital in evaluating a suspected ovarian mass. In postmenopausal women, serum CA-125 determinations further improve sensitivity and specificity. Once the presence of an ovarian mass is established, the crucial decision is whether to observe the patient or proceed with surgical removal. If surgical removal is indicated, the proper procedure is important for staging and initiation of effective therapy. While many surgeons are now using the less invasive laparoscopic approach, the standard of care continues to be a laparotomy with either an ovarian cystectomy or oophorectomy. Future clinical research in the management of adnexal masses should focus on decreasing the number of patients undergoing a surgical procedure, and in patients who require surgery, further evaluation of laparoscopy as a safe, cost-effective means of treatment. Improved imaging techniques may allow for nonoperative management of probably benign ovarian neoplasms.	{ "pile_set_name": "PubMed Abstracts" }
Decrease in phosphorylation of ERK following decreased expression of NK cell-activating receptors in human NK cell line exposed to asbestos. YT-CB5, which had been continuously cultured with chrysotile B (CB)asbestos, showed impaired cytotoxicity with decreased expression of NKG2D and 2B4 NK cell-activating receptors. In the present study, the phosphorylation of extracellular signal-regulated kinase (ERK), which is known to induce degranulation downstream of many NK cell-activating receptors, was examined in YT-CB5 by flow cytometry and compared with the control line YT-Org. YT-CB5 exhibited impaired phosphorylation of ERK1/2 induced by the recognition of K562 cells, downstream of a process mediated by Src family kinase and phosphoinositide 3-kinase. YT-CB5 also exhibited impaired phosphorylation of ERK1/2 following incubation with K562 cells in the presence of anti-2B4 antibodies, where co-stimulation by 2B4 augmented the phosphorylation of ERK1/2 in YT-CB5 to a similar degree as in YT-Org. The phosphorylation of ERK1/2 induced by an inhibitor against phosphatase (PP) 1 and PP2A was also lower in YT-CB5 compared with YT-Org. Moreover, bead-bound antibodies to NKG2D, which contribute to cytotoxicity against K562 cells, induced negligible phosphorylation of ERK1/2 in YT-CB5, although antibodies to 2B4 induced a comparatively greater level of phosphorylation. Additionally, peripheral blood (PB-) NK cells with low expression of NKG2D showed lower phosphorylation of ERK1/2 mediated by anti-NKG2D antibodies compared with PB-NK cells with high expression of NKG2D. These results indicate that signal transduction events leading to the phosphorylation of ERK is impaired in YT-CB5 due to decreased expression of NKG2D. Further studies are required to clarify whether this suppressive effect of asbestos exposure on NK cells might promote lung cancer and mesothelioma in people who have inhaled asbestos.	{ "pile_set_name": "PubMed Abstracts" }
Peptide profiling of the route from Mahoney to Sabin, and return. In order to define poliovirus (PV) neurovirulence at the molecular level, we comparatively analyze the primary amino acid sequence of Mahoney, a neurovirulent PV strain, versus (i) Sabin, an attenuated PV strain, and (ii) IS1, a PV isolate obtained in temporal association to a paralysis event from a polio vaccinated subject. We identify and describe 12 pentapeptides that, originally present in the Mahoney sequence, are changed in the non-neurovirulent Sabin strain, and, successively, restored in IS1 strain.	{ "pile_set_name": "PubMed Abstracts" }
Methylated purine bases in hepatic and colonic RNA of rats treated with 1,2-dimethylhydrazine. Administration of 1,2-dimethylhydrazine (DMH) to rats produces colon cancer. The mechanism by which this agent induces colon cancer is unclear. This investigation was conducted to assess the effect of DMH on the hepatic RNA and the colonic RNA of rats. DMH (300 mg/kg body wt) was administered to rats by ip injections. After 24 h, the hepatic RNA and the colonic RNA were isolated and their component purine bases were analyzed by HPLC. DMH treatment resulted in the formation of 1-methyladenine, 1-methylguanine, N2-methylguanine, O6-methylguanine, and 7-methylguanine in RNA. These methylated products may play a role in cellular injury produced by DMH.	{ "pile_set_name": "PubMed Abstracts" }
Prescribers' indications for drugs in childhood: a survey of five European countries (Spain, France, Bulgaria, Slovakia and Russia). Indication-based, in comparison to diagnoses-based, drug utilization studies in children are scarce in the literature. To determine the adequacy of the prescriber's indications for specific drug treatments compared to the current literature in five different European countries; and to show the possibilities of performing indication-based drug utilization studies. a descriptive, cross-sectional, international study. Randomly selected sample of 12,264 paediatric outpatients seen in consultation rooms attended by paediatricians or general practitioners. Data on patient demographics, diagnoses, and pharmacological treatment, with therapeutic indications for each drug, were collected in pre-designed forms. Diagnoses and indications were coded using the ICD-9 and drugs according to the ATC classifications. Indications were registered for every drug prescribed in all locations. Antibiotic indications considered incorrect (common cold, upper respiratory tract infections, viral infections, general symptoms or "not specified") accounted from 24.1% of the total antibiotics prescribed in Tenerife to 67.4% in Slovakia. Incorrect indication of first-choice antibiotics prescribed in acute otitis media and tonsillitis ranged from 28.9% of total antibiotics use in Russia to 75.4% in Tenerife. Correct antibiotic indications ranged from 23.4% of total antibiotics used in Slovakia to 65.7% in Tenerife. Aspirin use in febrile viral conditions was detected mainly in Toulouse and Russia. The main areas for improvement detected were high use of mucolytics, prescription of aspirin in potential or established viral infections, overuse of antibiotics and identification of specific patterns of incorrect antibiotic prescription and clinical entities associated with each location.	{ "pile_set_name": "PubMed Abstracts" }
Cyclosporine blocks the induction of class I and class II MHC products in mouse kidney by graft-vs-host disease. The ability of cyclosporine to prevent the increase in Ia and H-2K expression that occurs in mice with graft-vs-host disease (GVHD) was examined by means of absorption, indirect immunofluorescent staining (IIF), and indirect immunoperoxidase staining (IIP). Acute GVHD was induced in irradiated C3H/HeJ mice (H-2k) by injections of bone marrow and spleen cells from C57BL/6J mice (H-2b). Ten days after induction of acute GVHD, the spleens of mice not receiving cyclosporine expressed only donor Ia, reflecting their reconstitution by donor cells. The kidneys of such mice had a 10-fold increase in host Ia and H-2K expression, as previously reported. Treatment with cyclosporine reduced the amount of donor Ia and H-2K in spleens, and prevented the enhanced expression of recipient Ia and H-2K in kidneys in a dose-dependent manner. IIF or IIP staining showed that the principal change was in kidney tubules, where the induction of Ia and H-2K expression was greatly diminished. Cyclosporine administered to normal mice did not alter Ia expression except at high doses, at which it decreased Ia expression in kidneys and in spleens. The results suggest that prevention of enhanced MHC product expression could be part of the immunosuppressive actions of cyclosporine.	{ "pile_set_name": "PubMed Abstracts" }
First Report of Sclerotinia sclerotiorum on Common Ragweed (Ambrosia artemisiifolia) in Europe. Common ragweed (Ambrosia artemisiifolia L.) is reported as a host of Sclerotinia sclerotiorum (Lib.) de Bary in North America (2,4), but not in Europe. A Hungarian survey of fungal diseases of ragweed in 1994 did not find sclerotinia rot of common ragweed (A. artemisiifolia var. elatior (L.) Descourt.) (1). In autumn 1998, mature ragweed plants, 1 to 1.5 m tall, were collected from the borders of four sunflower (Helianthus annuus L.) fields in which sclerotinia rot of sunflower was frequently observed during the season, and also from six other roadside sites in Hungary. Ragweed plants exhibiting symptoms characteristic of sclerotinia rot, i.e., wilting foliage and light brown, dry lesions on the stems, were found only near two sunflower fields. Black, round to irregular or oblong sclerotia were also observed on the infected ragweed plants both externally on the stem lesions and internally, in the pith cavity. Sclerotia measured up to 5 mm in diameter and were 5 to 14 mm long. After isolation on potato dextrose agar, the pathogen produced abundant aerial mycelium and large sclerotia characteristic of S. sclerotiorum. To confirm pathogenicity, potted seedlings and mature plants of ragweed were inoculated in the greenhouse with autoclaved wheat grains colonized with mycelia of S. sclerotiorum placed 0.5 to 1 cm from the collar of the test plants. Seedlings were killed in 2 to 3 days while mature plants wilted after 5 to 6 days. In a field test, six mature plants were inoculated by attaching mycelial disks to their stems with Parafilm. These plants wilted 12 to 14 days after inoculation. The pathogen was reisolated from all diseased plants. This is the first report of S. sclerotiorum on common ragweed in Europe. Nonsclerotial mutants of the fungus (3) are being produced to be tested as potential biocontrol agents of common ragweed, which has become not only the most widespread, but also the most important allergenic plant species in Hungary since the early 1990s. References: (1) Gy. Bohár and L. Vajna. Nōvényvédelem 32:527, 1996. (2) G. J. Boland and R. Hall. Can. J. Plant Pathol. 16:93, 1994. (3) G. J. Boland and E. A. Smith. Phytopathology 81:766, 1991.(4) D. F. Farr et al. 1989. Fungi on Plants and Plant Products in the United States. American Phytopathological Society, St. Paul, MN.	{ "pile_set_name": "PubMed Abstracts" }
Genetic characterization of the rotaviruses associated with a nursery outbreak. G1P[6] rotaviruses were demonstrated previously to be associated with the neonatal nursery outbreak of gastroenteritis in Changhua Christian Hospital that is located in the central region of Taiwan, from September 1994 to May 1995. Meanwhile, rotaviruses were detected in children hospitalized for acute gastroenteritis. Our study characterizes the rotaviruses associated with the nursery outbreak by using genetic approaches. Nucleotide sequence analysis revealed that the VP7 genes of the nursery rotaviruses were distinct from those of the strains circulating in the community. The G1P[6] rotaviruses recovered from the nursery were closely related to another neonatal G1P[6] strain from the northern region of Taiwan in both the VP4 and VP7 genes. The VP4 genes of these nursery strains differed from those of the P[6] human reference strains 1076, M37, RV3, and ST3. Apparently, these nursery rotaviruses were distinct from the strains circulating in the community and seemed to be a variant when compared with P[6] strains reported previously.	{ "pile_set_name": "PubMed Abstracts" }
Legacy and emerging pollutants in the Gulf of Gdańsk (southern Baltic Sea) - loads and distribution revisited. Coastal marine areas of densely populated countries are exposed to a wide array of human activities having an impact on their ecological status. The Baltic Sea is particularly susceptible to pollution by hazardous substances (limited water exchange, shallowness, and large catchment area). Polish media regularly reports ecological catastrophes in the Gulf of Gdańsk area caused by eg. shipwrecks leaking. Thus, there is a need of a broad scientific based report on recent contaminant loads and distribution. In this review paper, we report loads of contaminants from different obvious and non-obvious sources. We also gather data on legacy and new emerging contaminant concentrations measured in the Gulf of Gdańsk within the last decade (2008-2018). The paper also includes available biological effect measurements performed recently as well as a summary of needs and gaps to be filled for the development of reliable risk assessment.	{ "pile_set_name": "PubMed Abstracts" }
Protein requirement of young adult Nigerian females on habitual Nigerian diet at the usual level of energy intake. A short-term N balance study was conducted in twelve healthy female adults aged 21-32 years to determine their protein requirement. Four dietary protein levels (0.3, 0.4, 0.5 and 0.6 g protein/kg per d) were used. Energy intake of the subjects was kept constant at 0.18 MJ/kg per d. All subjects maintained their normal activity throughout the study period. N excretion was determined from the measurements of N in a total collection of urine, faeces, sweat and menstrual fluid for each dietary period. N balance during the four protein levels were -15.15 (SD 5.95), -5.53 (SD 6.71), +6.15 (SD 4.76) and +12.05 (SD 8.63) mg N/kg per d for 0.3, 0.4, 0.5 and 0.6 g protein/kg per d respectively. The calculated average N requirements from regression analysis was 76.0 (SD 3.37) mg N/kg per d (0.48 g protein/kg per d). The estimate of allowance for individual variation to cover the 97.5% population was 95 mg N/kg per d (0.6 g protein/kg per d). The net protein utilization (NPU) of the diet was 0.55. When compared with a similar study with men, there was a significant difference in the protein requirement between sexes. Thus, the unjustifiable sex difference in the protein allowance recommended by the Food and Agriculture Organization/World Health Organization/United Nations University (1985) Expert Consultation group must be reviewed.	{ "pile_set_name": "PubMed Abstracts" }
[Effects of aging and hyperlipidemia on plasma osteopontin level]. In order to elucidate a possible mechanism for accelerated atherogenesis as well as enhanced vascular calcification observed during the normal aging process, we measured plasma osteopontin (OPN) levels and examined their relation to aging and certain disease parameters. In all cases examined, no significant relation was found between the plasma OPN level and age, body mass index, blood pressure, plasma levels of glucose and insulin, serum levels of creatinine, triglyceride, and high density lipoprotein cholesterol. On the other hand, a significant negative correlation was found between the plasma OPN level and serum total cholesterol concentration (n = 78, r = -0.355, p = 0.0014). The serum level of low density lipoprotein (LDL) cholesterol, calculated by the formula of Friedewald, also showed a significant negative correlation to the plasma OPN level (n = 78, r = -0.301, p = 0.0075). In cases without diabetes mellitus and hypertension, a significant positive correlation was found between the plasma OPN level and age (n = 22, r = 0.445, p = 0.0378). It is postulated that OPN plays a negative regulatory role in the development of vascular calcification. Therefore, the observed negative relationship between the plasma OPN level and the serum levels of total cholesterol and LDL cholesterol, suggests a possibility that hypercholesterolemia facilitates vascular calcification by suppressing OPN synthesis. On the other hand, in non-diabetic and normotensive cases, the positive relationship between the plasma OPN level and age may reflect a defense mechanism against age-related increase of vascular calcification.	{ "pile_set_name": "PubMed Abstracts" }
Effect of an injury prevention program on muscle injuries in elite professional soccer. Due to the continual physical, physiological, and psychological demands of elite level soccer increasing the incidence and risk of injuries, preventative training programs have become a common feature of soccer players training schedule. The aim of the current investigation was to examine the effectiveness of a structured injury prevention program on the number of muscle injuries and the total number of injuries within elite professional soccer. The present study was conducted over 2 consecutive seasons, of which the first (2008-2009) being the intervention season and the second the control season (2009-2010). In total, 26 and 23 elite male professional soccer players competing within the Scottish Premier League and European competition participated. The training program was performed twice weekly for the entirety of the season (58 prevention sessions). The results revealed an increase in the total number of injuries within the intervention season (88 vs. 72); however, this was largely due to the greater number of contusion injuries sustained within the intervention season (n = 44) when compared with control season (n = 23). Significantly less muscle injuries were observed during the intervention season (moderate effect), and this occurred concomitant with a bigger squad size (large effect, p < 0.001). The findings from this study identify that a multicomponent injury prevention-training program may be appropriate for reducing the number of muscle injuries during a season but may not be adequate to reduce all other injuries.	{ "pile_set_name": "PubMed Abstracts" }
Spontaneous reduction of intestinal intussusception in five young dogs. Five, 3- to 8-month-old dogs had clinical and ultrasonographic findings consistent with intestinal intussusception, but findings were negative on exploratory celiotomy. Ultrasonography had revealed a target-like mass (median maximal diameter 25 mm) on transverse section and multiple hyperechoic or hypoechoic parallel lines (median length 36.8 mm) on longitudinal section. Blood flow in the intussuscepted bowel was detected in most of the cases. In dogs with similar findings, the authors recommend ultrasonographic reexamination of the abdomen after general anesthesia (but before surgery) to exclude spontaneous reduction of the intussusception.	{ "pile_set_name": "PubMed Abstracts" }
[The asymmetrical brain--asymmetrical consciousness]. To substantiate a term "asymmetry of consciousness", to throw light on it are compared disturbances of consciousness in dextral and sinistral persons with selective lesions in the right or left brain hemispheres. Is supposed a fundamental role of the spatial-temporal factors. By them are mediated relations "brain-consciousness", asymmetry of consciousness. The latter is expressed in dextral persons by an opposite spatial-temporal organization of psycho-sensory (which are realized in individual present and past) and psychomotor (which are displayed in external present and future) processes. This contrast is not observed in sinistrals or only in some of them. Is assumed a probability of evolution of space and time with appearance of their forms having been absent before the human level. They are presumably described by the authors as individual space and time, on the one hand being determined by the functioning brain of each dextral and sinistral person, on the other hand defining the limits and limitations of the mental abilities of each person. Individual space and time may be other in a sinistral person than in a dextral one.	{ "pile_set_name": "PubMed Abstracts" }
Potential job facilitation benefits of "water cooler" conversations: the importance of social interactions in the workplace. This study looked at the extent to which personality and cultural factors predicted participants' perceptions of the importance private interactions played in the workplace. The 134 participants read a vignette (where a new employee socially interacted at low or high levels with co-workers) and completed the Big Five Inventory, Social Axioms Survey, and questions concerning expected workplace experiences. Results indicated employees who engaged in high levels of private interaction with co-workers were expected to be better liked, to receive better performance evaluations, were more likely to receive co-worker assistance, and were thought to be more likely chosen for future projects. However, the personality and social axiom variables studied did not significantly interact with social interaction to influence expectations of workplace outcomes.	{ "pile_set_name": "PubMed Abstracts" }
Sternum screw: analysis of a novel approach to the closure of the chest after surgery. To show the benefits of using a novel approach to closure of the median sternotomy through a mechanical model and mechanical testing. Simple cannulated screws are placed on either side of the sternotomy. Conventional stainless steel wire is passed through the cannula of each screw and the sternotomy is closed in the usual manner. Hertzian contact analysis was used to estimate the stress between the wire and the sternum. Mechanical testing was used to compare using wire on its own with a sternum screw plus wire. Ten samples of balsa wood (sternum substitute) had wire placed through a hole in them, while a further ten samples were fitted with a cannulated screw and had wire passed through the screw cannula. The wire was connected to a materials testing machine, which applied tension to the wire until the wire or screw cut through the wood. The analysis showed that the mean stress between the wire and the sternum decreases with increasing wire diameter. At low diameters of wire the stress in the sternum can be comparable to the failure stress of bone. Using a cannulated screw reduces the stresses in the sternum. The mechanical testing showed that the wire cut through the wood at a mean load of 104 N, whereas the sternum screw cut through the wood at a mean load of 209 N (p = 0.007, Mann-Whitney Test). Closing a median sternotomy with cannulated screws plus wire should reduce the occurrence of sternal dehiscence.	{ "pile_set_name": "PubMed Abstracts" }
[The apoB genotype and the efficacy of hypolipidemic therapy]. Several men were examined for association between restriction fragment length polymorphism (RELP) Xba I (exon 26) number of tandem repeats in 3'-hypervariable region of the apolipoprotein-B gene and serum levels of cholesterol and triglyceride. These two types of polymorphism were studied. An association of the Xba I site and alleles containing more repeats in the 3'-hypervariable region with higher cholesterol and triglyceride was observed. 32 patients with CHD aged 24-56 years were examined. All the patients are males with clinical picture of CHD (stable angina pectoris of II-III functional classes) and dyslipoproteinemia of II a, II b and IV types by D. S. Fredrickson. Xba-I polymorphism of apo-B gene was detected by DNA polymerase reaction method. The following Xba-I genotypes were distinguished: X1X1 (absence of Xba I site); X1X2 (heterozygosity on Xba I site) and X2X2 (homozygosity on Xba-I site). Lipantil (fenofibratte) was prescribed in a dose of 300 mg daily after meals (100 mg three times a day). Data obtained show that DNA polymorphism of apo-B gene not only influences plasma lipids concentration but also determines effectiveness of hypolipidemic therapy. Hypolipidemic effect of lipantil depends on Xba-I site presents in apo-B gene and is significantly expressed in homozygous patients with X1X1 genotype.	{ "pile_set_name": "PubMed Abstracts" }
Disruption of redox homeostasis in the transforming growth factor-alpha/c-myc transgenic mouse model of accelerated hepatocarcinogenesis. In previous studies we have demonstrated that transforming growth factor (TGF)-alpha/c-myc double transgenic mice exhibit an enhanced rate of cell proliferation, accumulate extensive DNA damage, and develop multiple liver tumors between 4 and 8 months of age. To clarify the biochemical events that may be responsible for the genotoxic and carcinogenic effects observed in this transgenic model, several parameters of redox homeostasis in the liver were examined prior to development of hepatic tumors. By 2 months of age, production of reactive oxygen species, determined by the peroxidation-sensitive fluorescent dye, 2',7'-dichlorofluorescin diacetate, was significantly elevated in TGF-alpha/c-myc transgenic hepatocytes versus either wild type or c-myc single transgenic cells, and occurred in parallel with an increase in lipid peroxidation. Concomitantly with a rise in oxidant levels, antioxidant defenses were decreased, including total glutathione content and the activity of glutathione peroxidase, whereas thioredoxin reductase activity was not changed. However, hepatic tumors which developed in TGF-alpha/c-myc mice exhibited an increase in thioredoxin reductase activity and a very low activity of glutathione peroxidase. Furthermore, specific deletions were detected in mtDNA as early as 5 weeks of age in the transgenic mice. These data provide experimental evidence that co-expression of TGF-alpha and c-myc transgenes in mouse liver promotes overproduction of reactive oxygen species and thus creates an oxidative stress environment. This phenomenon may account for the massive DNA damage and acceleration of hepatocarcinogenesis observed in the TGF-alpha/c-myc mouse model.	{ "pile_set_name": "PubMed Abstracts" }
Evaluation of choroidal and retinal thickness measurements in adult hemodialysis patients using spectral-domain optical coherence tomography. To assess the effect of hemodialysis on retinal and choroidal thicknesses using spectral-domain optical coherence tomography (SD-OCT). In this prospective interventional study, 25 hemodialysis patients (17 male, 8 female) were enrolled. All participants underwent high-speed, high-resolution SD-OCT (λ=840 mm; 26.000 A-scans/s; 5 µm resolution) before and after hemodialysis. Choroidal thickness was measured perpendicularly from the outer edge of the retinal pigment epithelium to the choroid-sclera boundary at the fovea and at five additional points: 500 µm and 1000 µm nasal to the fovea and 500 µm, 1000 µm, and 1500 µm temporal to the fovea. Two masked physicians performed the measurements. Choroidal and retinal thicknesses before and after hemodialysis were compared. The median choroidal thicknesses before and after hemodialysis were 182 µm (range, 103-374 µm) and 161 µm (range, 90-353 µm), respectively (P<0.001). The median retinal thicknesses were 246 µm (range, 179-296 µm) before and 248 µm (range, 141-299 µm) after hemodialysis (P>0.05). Systolic arterial pressure, diastolic arterial pressure, mean arterial pressure, heart rate, and ocular perfusion pressure significantly decreased after hemodialysis (P<0.001). Intraocular pressure did not vary significantly (P=0.540). Hemodialysis seems to cause a significant decrease in choroidal thickness, whereas it has no effect on retinal thickness. This significant decrease in choroidal thickness might be due to the extensive fluid absorption in hemodialysis, which could result in decreased ocular blood flow.	{ "pile_set_name": "PubMed Abstracts" }
Determinants and impact of sleep duration in children and adolescents: data of the Kiel Obesity Prevention Study. This study investigates determinants of sleep duration and its impact on nutritional status, resting energy expenditure (REE), cardiometabolic risk factors and hormones in children/adolescents. In 207 girls and 207 boys (13.0+/-3.4 (6.1-19.9) years) body mass index standard deviation score (BMI SDS), waist circumference (WC) z-score, body composition (air-displacement plethysmography), REE (ventilated hood system; n=312) and cardiometabolic risk factors/hormones (n=250) were assessed. Greater than 90th percentile of BMI/WC references was defined as overweight/overwaist. Sleep duration, media consumption (TV watching/computer use), physical activity, dietary habits, parental BMI, socio-economic status and early infancy were assessed by questionnaire. Short sleep was defined as <10 h per day for children <10 years and otherwise <9 h per day. Total 15.9% participants were overweight, mean sleep duration was 8.9+/-1.3 h per day. Age explained most variance in sleep (girls: 57.0%; boys: 41.2%) besides a high nutrition quality score (girls: 0.9%) and a low media consumption (boys: 1.3%). Sleep was inversely associated with BMI SDS/WC z-score (girls: r=-0.17/-0.19, P<0.05; boys: r=-0.21/-0.20, P<0.01), which was strengthened after adjusting for confounders. Short vs long sleep was associated with 5.5-/2.3-fold higher risks for obesity/overwaist (girls). After adjusting for age, REE (adjusted for fat-free mass) was positively associated with sleep in boys (r=0.16, P<0.05). Independently of age and WC z-score, short sleep was associated with lower adiponectin levels in boys (11.7 vs 14.4 microg/ml, P<0.05); leptin levels were inversely related to sleep in girls (r=-0.23, P<0.05). Homoeostasis model assessment-insulin resistance (r=-0.20, P<0.05) and insulin levels (r=-0.20, P<0.05) were associated with sleep (girls), which depended on WC z-score. Age mostly determined sleep. Short sleep was related to a higher BMI SDS/WC z-score (girls/boys), a lower REE (boys), higher leptin (girls) and lower adiponectin levels (boys).	{ "pile_set_name": "PubMed Abstracts" }
The effect of early epidural versus early intravenous analgesia use on labor progression: a natural experiment. The purpose of this study was to compare the effect of early epidural with the use of early intravenous analgesia on labor progression. We systematically selected singleton, nulliparous term pregnancies with a spontaneous labor and analgesia placement <or=4 cm who were delivered at a hospital where the rate of epidural analgesia in labor increased from 1% (before) to 84% (after) in 1 year (a natural experiment). In the before period (n=223 pregnancies), 98% of women used intravenous analgesia, and 2% of women used epidural analgesia. In the after period (n=278 pregnancies), 92% of women used epidural analgesia, and 8% of women used intravenous analgesia. The median duration of labor by each centimeter of cervical dilation was computed and used as a measurement of labor progression. After adjustment had been made for confounders, women in the after period had a slower labor progression only from 4 to 5 cm, compared with those women in the before period. Interestingly, the process of labor admission and epidural analgesia placement, rather than analgesia use per se, appeared to explain most of the slowdown. No significant difference in the rest of the active phase was observed between the 2 groups. Our data support recent American College of Obstetricians and Gynecologists guidelines that the restraining use of epidural analgesia at <4 cm of cervical dilation is unnecessary.	{ "pile_set_name": "PubMed Abstracts" }
Laparoscopic proximal gastrectomy for gastric neoplasms. For cancers of the distal gastroesophageal junction or the proximal stomach, proximal gastrectomy can be performed. It is associated with several perioperative benefits compared with total gastrectomy. The use of laparoscopic proximal gastrectomy (LPG) has become an increasingly popular approach for select tumors. We describe our method of LPG, including the preoperative work-up, illustrated depictions of the key steps of the surgery, and our postoperative pathway. A total of 6 patients underwent LPG between July, 2013 to June, 2017. Five patients had early-stage adenocarcinoma, and 1 patient had a gastrointestinal stromal tumor. The median age of the cohort was 70, and each patient had significant comorbidities. Conversion to open was required for 1 patient. All patients had negative final margins and an adequate lymph node dissection (median number of nodes examined was 15, range 12-22). The median postoperative length of stay was 7 days (range 4-7). Two patients developed anastomotic strictures requiring intervention, and 1 patient experienced significant reflux. At a median follow-up of 11 months, there was 1 recurrence. Three patients were alive without evidence of disease, and 2 patients died from other causes. For carefully selected patients, LPG is a safe and reasonable alternative to total gastrectomy, which is associated with similar oncologic outcomes and low morbidity.	{ "pile_set_name": "PubMed Abstracts" }
Experimental study of skin-sensitizing antibodies after aerosol and subcutaneous immunization. The reaction of passive skin anaphylaxis was used to investigate the accumulation and the dynamics of skin-sensitizing antibodies (SSA) in the sera of guinea pigs immunized with botulin anatoxin by means of different methods: the aerosol and the subcutaneous method. In the aerosol-immunized animals (unlike the subcutaneously immunized ones) SSA appeared in a negligible amount only after the fourth immunization and disappeared rapidly.	{ "pile_set_name": "PubMed Abstracts" }
Complications and patient-reported outcome after hip fracture. A consecutive annual cohort study of 664 patients. The aim of every patient with hip fracture is to regain previous function but we know little about the outcome, especially patient-reported outcome. We wanted to investigate what factors influence the result one year after hip fracture, including fast-track for hip fracture patients, as well as investigating the patients' satisfaction with their rehabilitation and to what degree they regained their pre-fracture function. All patients (>20 years, non-pathological fracture, residents in the catchment area, n=664) having surgery for hip fracture at our hospital during 2011 were included in a retrospective cohort study. From medical records, information was gathered about pre-fracture condition as well as fracture type, surgical details, length of stay and whether the patient entered the hospital through the fast-track system. Medical records were scrutinised for general complications up to six months and for local complications up to one year after surgery. A postal questionnaire was sent one year after surgery inquiring about health status, pain and satisfaction along with multiple-choice questions regarding mobility and rehabilitation. Variables were analysed with linear regression or the proportional odds model. The most common general complications were new falls, pneumonia and new fractures. Deep infection was the most frequent local complication. The only significant effect of the fast-track system was shorter time to surgery (78 vs. 62% had surgery within 24h, p<0.001). A total of 29% reported to have regained their previous mobility and 30% considered the rehabilitation to be adequate. Mean value for pain VAS was 24 (SD 22) and for satisfaction 28 (SD 25). Absence of general and local complications correlated to satisfaction and hip pain. General complications correlated to loss of function. Higher age correlated to inadequate rehabilitation. General complications seem to be the major risk factor, being the only factor affecting functional outcome and together with local complications affecting pain and satisfaction. To avoid general complications, co-operation between orthopaedic surgeons and internists may be crucial in the aftercare of hip fracture patients. A majority did not receive adequate rehabilitation and efforts need to be made to improve the rehabilitation process.	{ "pile_set_name": "PubMed Abstracts" }
Reflections on ethical issues in psychopharmacology: an American perspective. Psychopharmacology has revolutionized psychiatric practice but raises a number of ethical issues. This review from an American perspective first describes ethics analyses and attempts to portray the ethical practitioner. Pressures that interfere with appropriate prescribing come from outside the prescriber and from within, including from insurers, other treatment staff and the prescriber's own will to act for the patient. Clinicians also face binds in which alternate choices seem to have merit and leave the prescriber feeling pulled in contradictory directions, frequently related to risk-benefit dilemmas. The ethics of psychopharmacology poses many questions that cannot yet be answered at the current state of the field. Pharmacology also seems to promote extremes of attitudes, such as "All such drugs are poisons" and the like. This review then provides some risk management principles, and concludes that such a review, though not comprehensive, may serve to open questions that are not always considered by clinicians.	{ "pile_set_name": "PubMed Abstracts" }
A monoclonal antibody that specifically recognizes a glucuronic acid 2-sulfate-containing determinant in intact chondroitin sulfate chain. Monoclonal antibodies produced against chick embryo limb bud proteoglycan (PG-M) were selected for their ability to recognize determinants on intact chondroitin sulfate chains. One of these monoclonal antibodies (IgM; designated MO-225) reacts with PG-M, chick embryo cartilage proteoglycans (PG-H, PG-Lb, and PG-Lt), and bovine nasal cartilage proteoglycan, but not with Swarm rat chondrosarcoma proteoglycan. The reactivity of PG-H to MO-225 is not affected by keratanase digestion but is completely abolished after chondroitinase digestion. Competitive binding analyses with various glycosaminoglycan samples indicate that the determinant recognized by MO-225 resides in a D-glucuronic acid 2-sulfate(beta 1----3)N-acetylgalactosamine 6-sulfate disaccharide unit (D-unit) common to antigenic chondroitin sulfates. A tetrasaccharide trisulfate containing D-unit at the reducing end is the smallest chondroitin sulfate fragment that can inhibit the binding of the antibody to PG-H. Decreasing the size of a D-unit-rich chondroitin sulfate by hyaluronidase digestion results in progressive reduction in its inhibitory activity. The results suggest that the epitope has a requirement for a long stretch of a disaccharide-repeating structure for a better fit to the antibody.	{ "pile_set_name": "PubMed Abstracts" }

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