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In addition to the unusual global genomic features described above, UCYN-A also lacks several fundamental metabolic pathways, including the tricarboxylic acid (TCA) cycle, the Calvin cycle for carbon fixation, biosynthetic pathways for several amino acids, and purine biosynthesis (Figure 1b). Given the complete lack of... |
On the basis of their data, Tripp et al. [1] propose an interesting scheme for electron flow and energy conservation involving external electron donors (organic carbon or H2), nitrogenase (a byproduct of which is H2), hydrogenases, oxidative phosphorylation, PSI-catalyzed light-driven photophosphorylation, and a variet... |
As is common in such analyses, what is lacking in the full genome sequence of UCYN-A is as revealing as what is present. It is also worth noting that this study is one of the first to successfully demonstrate full genome assembly from a flow-sorted cell population from a complex community, enabled by MDA and pyrosequen... |
1. Tripp HJ, Bench SR, Turk KA, Foster RA, Desany BA, Niazi F, Affourtit JP, Zehr JP: Metabolic streamlining in an open-ocean nitrogen-fixing cyanobacterium. |
Nature 2010, 464:90-94. PubMed Abstract | Publisher Full Text OpenURL |
2. Capone DG, Burns JA, Montoya JP, Subramaniam A, Mahaffey C, Gunderson T, Michaels AF: Nitrogen fixation by Trichodesmium spp.: An important source of new nitrogen to the tropical and subtropical North Atlantic. |
Global Biogeochem Cycles 2005, 19:GB2024. Publisher Full Text OpenURL |
3. Zehr JP, Waterbury JB, Turner PJ, Montoya JP, Omoregie E, Steward GF, Hansen A, Karl DM: Unicellular cyanobacteria fix N2 in the subtropical North Pacific Ocean. |
Nature 2001, 412:635-638. PubMed Abstract | Publisher Full Text OpenURL |
4. Montoya JP, Holl CM, Zehr JP, Hansen A, Villareal TA, Capone DG: High rates of N2 fixation by unicellular diazotrophs in the oligotrophic Pacific Ocean. |
Nature 2004, 430:1027-1032. PubMed Abstract | Publisher Full Text OpenURL |
5. Rappe MS, Giovannoni SJ: The uncultured microbial majority. |
Annu Rev Microbiol 2003, 57:369-394. PubMed Abstract | Publisher Full Text OpenURL |
6. Giovannoni S, Stingl U: The importance of culturing bacterioplankton in the 'omics' age. |
Nat Rev Microbiol 2007, 5:820-826. PubMed Abstract | Publisher Full Text OpenURL |
7. Ishoey T, Woyke T, Stepanauskas R, Novotny M, Lasken RS: Genomic sequencing of single microbial cells from environmental samples. |
Curr Opin Microbiol 2008, 11:198-204. PubMed Abstract | Publisher Full Text OpenURL |
8. Zehr JP, Bench SR, Carter BJ, Hewson I, Niazi F, Shi T, Tripp HJ, Affourtit JP: Globally distributed uncultivated oceanic N2-fixing cyanobacteria lack oxygenic photosystem II. |
Science 2008, 322:1110-1112. PubMed Abstract | Publisher Full Text OpenURL |
9. Zehr JP, Bench SR, Mondragon EA, McCarren J, DeLong EF: Low genomic diversity in tropical oceanic N2-fixing cyanobacteria. |
Proc Natl Acad Sci USA 2007, 104:17807-17812. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL |
10. Moisander PH, Beinart RA, Hewson I, White AE, Johnson KS, Carlson CA, Montoya JP, Zehr JP: Unicellular cyanobacterial distributions broaden the oceanic N2 fixation domain. |
Science 2010, 327:1512-1514. PubMed Abstract | Publisher Full Text OpenURL |
1. Religion & Spirituality |
Ashtalakshmi - The 8 Forms of Lakshmi |
7 of 9 |
Ashtalakshmi - The 8 Forms of Lakshmi |
Veera-Lakshmi: As the names suggests (Veera = valor or courage), this form of Goddess Lakshmi is the bestower of courage and strength, and power. Veera-Lakshmi is worshiped to gain valor and strength to overpower formidable adversaries in war or simply overcome the difficulties of life, and lead a life of stability. Sh... |
Veera-Lakshmi or Dhairya-Lakshmi Prayer Song: Lyrics of the hymn or stotram dedicated to this form of Lakshmi |
Jayavaravarshini, Vaishnavi, Bhaargavi Mandhrasvaroopini, Manthramaye, Suraganapoojitha, Sreeghraphalapradha Jnaanavikaasini, Shaastramathe, Bhavabhayahaarini, Paapavimoochani Saadhujanaasritha Paadhayuthe, Jaya Jaya He, Madhusoodhana Kaamini Dhairyalakshmi, Jaya, Paalayamaam || |
Listen / Download - Ashta-Lakshmi Stotra (MP3) |
©2014 About.com. All rights reserved. |
Tuesday, March 4, 2014 |
Movies of 2013, Horror, pt3 |
This is the most awful part of the horror genre this year. |
You're Next, or "Is this supposed to be Funny? I am not sure I am getting this." |
Overall: 4/10 |
Did the villains draw straws for who got which mask? Cause one of them is a lamb. That is not very bad ass. |
This movie has a slightly misleading trailer that if I had been in a better mood I would have been pleased with how it subverted my expectations. |
I can actually see this movie being regarded positively by people. It has a good pace that establishes its characters well enough, but I just couldn't get into it. |
Since the appeal of this is the subversion of expectations I don't want to spoil anything, if you like slasher movies then you will like this. I am kind of softening to it a bit as it rolls around in my head. I doubt I will ever watch it again, but I can imagine myself looking back on this having judged i... |
The Purge, or "That's it? What was the point of the premise? This plot has been done a hundred times before." |
Overall: 4/10 |
Also, there is no distinct iconic bad guy in this movie, so emphasizing the masks is a little dishonest. |
This isn't "Friday the 13th", which has sort of a recognizable mask centered character. |
This is a basic home invasion movie. A group of creepy assholes are trying to get in and the residents have to take steps to protect themselves. This is the same movie as "You're Next" but with the pretense of having social commentary. |
It's social commentary is never explored, nobody talks about how the titular Purge affects the poor and homeless disproportionately, or how the social good of the annual violence mostly has to do with those who can afford to participate killing those who don't have the financial means to defend themselves. ... |
There is also a lag in logic, once the intruders realize that the people in the house have the means to defend themselves with guns and security systems they logically should just fuck off to find easier prey, why risk getting gunned down for the pleasure of killing a stranger? Also, how did they locate th... |
This is an interesting premise, but it is not used to any effect, and judging by the sequel trailer they have decided not to bother trying to comment and instead just trying to make the standard lost-in-the-wilderness horror chase movie, except the wilderness is the deserted city... Which makes it look like... |
MaMa (walked out), or "Startling: The Movie." |
Overall: 1/10 |
And the ghost in it looks really silly. |
Shitty. This thing could have been good but the laziness of how it executes its scares kill it dead. Piercing music stings coupled with a scary face on screen shock but they do not scare in an interesting way. you are not filled with dread, you are filled with tense anticipation for when you will be star... |
If I were to offer some constructive suggestions, I would say use James Watkins who made the very okay "The Woman in Black" which captured its tone easier, and had a better mystery element (even though it ended up not making a lick of sense. Which would mean taking the writer off the director's seat and th... |
Monday, March 3, 2014 |
Movies of 2013, Horror, pt2 |
The medium-good horror stuff this year. |
John Dies at the End, or "I feel I should take it easy on the shit effects because it is so charming and weird." |
Overall: 6/10 |
This movie was based on a book by a contributor to the online magazine, Cracked. |
Supposedly inspired by a night taking drugs. That last part I believe. |
"Let's get high off the blood of an Old One." Fantastic. That is a thing we should do. |
"Dies" is so weird, that I love it a lot more than I really should. |
This is probably one of the only movies in which I will say without question: it needed more money. The dialogue is funny, the acting is solid, tons of cool props, intriguing premise, such odd little episodes, and characters that you actually like and give a shit about. |
This thing is so in sync with how my sense of humor and action that I kind of love it, but the absolute climax of the movie looks and feels so cheap that it poisons the rest of the film a great deal. It is so obviously green screen, so poorly cut trying to hide the issues... It looks just terrible. If thi... |
If you like horror comedy ("Army of Darkness", "Todd and the Book of Pure Evil", "Hansel and Gretel Witch Hunters") then this is a must see. But if you dislike cheap and hokey stuff give it a pass, because it really requires you to buy into the idea of parallel worlds, topless cults, and Jamaican fortune t... |
V/H/S/2, or "Certainly better than the first one. Still a lot of room for improvement." |
Overall: 6/10 |
Not quite as visually offensive as its predecessor. |
When I watched the first "V/H/S" I frequently found myself having to look away from the screen because the constant camera movement made me ill. Not all of the short films grabbed me, and the framing device of assholes stealing videos and then inexplicably sitting down to watch some was so stupid and over ... |
While the short films featured vary in quality. The first story has the biggest issue in my opinion, a guy gets an optical implant that allows him to see ghosts, and acts as a recorder, then meets a woman with an ocular implant that allows her to hear them, and then ghosts attack... That is cool, but I kep... |
It will still cause you motion sickness from the constant camera movement. But I never had to look away from the screen. Big improvement, definitely worth having another sequel to further refine the franchise. The framing story was also better, though the idea of staying in a hazardous place to watch mov... |
Sunday, March 2, 2014 |
Movies of 2013, Horror, pt1 |
These were the good horror movies. |
The Conjuring, or "Based on actual events my ass." |
Overall: 7/10 |
I look forward to the inevitable franchise this spawns. |
James Wan is a skilled director who can frame a shot, pace a scene, and evoke performances from actors. Patrick Wilson is an underappreciated actor who has been in a lot of really good stuff, he yells with real conviction, and actually looks concerned when a situation calls for concern... You would think t... |
"The Conjuring" is an elaborate story by a married couple of charlatans, the Warrens, who worked as ghost hunters and paranormal investigators for several decades. They are notorious for making up "The Amityville Horror". |
Thankfully this film takes "realism" or any hope of committing to the "real" story and locks those uninteresting things in its creepy basement and commences to beat your sense of, "I could see that happening" with a truncheon. Instead they opt to just tell an engaging story plotted out really well. |
Exposition is woven into the narrative with clues at the start whose intrigue is satisfied as the story progresses... You know, like a mystery story should. Shocking, someone managed to write something that doesn't just tell you what is happening, nor do they fail to find out or fail to act on what they al... |
I should also point out the good acting from Vera Farmiga and Lili Taylor (who convincingly looks terrified). |
There are scenes in this that are absolutely bone chilling especially with the quasi hide and seek game featured prominently in the trailers. Simple things, that makes my skin goosepimply as I write this in the quiet of late hours. One of the better films I saw this year. |
Insidious: Chapter 2, or "There are like 3 or 4 movies that the writer and director must have been watching to assemble this thing." |
Overall: 8/10 |
And I look forward to the continuing adventures of the heroes in this movie. |
See the opening paragraph under "The Conjuring"? Repeat that for the opening here and add in how this is the best sequel to a horror film I have ever seen. |
The first "Insidious" was a fantastically paced movie with numerous scenes that were tense, creepy, and visually very pretty. This movie weaves its narrative into the previous film damn near flawlessly, making its own additions to the mythos and using simple behind the scenes production decisions to build ... |
What is more this thing apes stuff from other really good movies and blends them in. There is stuff from "The Shining" and "Silence of the Lambs" that make the central antagonist just fascinating. They also, through use of established supernatural elements explore back through time creating a very interes... |
There is only one complaint that I can point to in the technical department: they dub over an actress in the opening scenes of the movie with the voice of a much older actress. I have no idea why, I knew which character the actress was supposed to be a younger iteration of without the additional audio clue... |
Also, good job on the part of Rose Byrne. A good portion of the movie is her moving around the house and looking like she is going nuts. |
So those factors kept this from being a truly great movie, but I would definitely put it in the top 10 (maybe the top 5) of the year. Watch it back to back with the original and you probably wouldn't notice that they were produced nearly 3 years apart (the kid actors aged I suppose). I am probably being t... |
Tuesday, February 25, 2014 |
Goodbye Harold Ramis |
I like movies, but there is a particular movie that I have seen probably in the triple digits, and that is "Ghostbusters". One of its stars, Harold Ramis, died in the immediate past. And I feel bad. I still consider him to be one of my influences of saying something that spells doom or plight with a dry ... |
Here is the thing, he lived a full life, filled with interesting people. Ramis was able to express himself creatively far more than most people ever will be able to. He was wealthy, happy, and made others happy. We can talk about it being too soon, but it was a life well lived and he was old. He lived a... |
He did other stuff aside from "Ghostbusters", and it is also good. |
Aside from his family who feels the loss of his life personally and is in need of sympathy, people who like comedy in general should feel glad that he lived, and thankful, and that should be the end of it. His work will not disappear, his place in my childhood will not scab over. |
This is actually one of many celebrity deaths that have happened in my life, and the last one I really remember was Paul Walker, whose creative work was abysmal and should not be what he is remembered for, instead his charitable work is a much greater contribution to society and what should be lauded. |
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