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The patients were informed about the surgical procedures to be performed and provided written consent. The research adhered to the ethical principles stated in the Declaration of Helsinki. Prior to commencing the study, the necessary approval was sought and obtained from the Clinical Research Ethics Committee of …… Uni...
38648458_p1
38648458
Methods
3.844963
biomedical
Study
[ 0.9763303399085999, 0.023265032097697258, 0.00040458462899550796 ]
[ 0.9712570309638977, 0.024281837046146393, 0.0006755272042937577, 0.003785629291087389 ]
en
0.999996
The specimens were immersed in containers filled with 10% formalin and then embedded in paraffin. Thin sections measuring 4 µm were extracted from the samples and placed on glass slides with barcodes for identification. Following the process of deparaffinization and rehydration, the sections were subjected to histopath...
38648458_p2
38648458
Immunohistochemistry
4.018377
biomedical
Study
[ 0.9992503523826599, 0.0005243247142061591, 0.0002252706472063437 ]
[ 0.9555733799934387, 0.04263880103826523, 0.001180301420390606, 0.0006075592245906591 ]
en
0.999997
We used primary antibodies against CD44 (1:50 dilution) and E-cadherin (1:200 dilution), and a human monoclonal antibody against PCNA (1:100 dilution) for antigen retrieval. Histopathological and immunohistochemical staining findings were reported using a Nikon light microscope and an image analysis system (Nikon Instr...
38648458_p3
38648458
Immunohistochemistry
4.128517
biomedical
Study
[ 0.9994326233863831, 0.0003504198684822768, 0.00021693222515750676 ]
[ 0.9992528557777405, 0.00036659400211647153, 0.0003078640147577971, 0.00007272900984389707 ]
en
0.999998
For continuous variables, the data were presented as either the mean ± standard deviation or the median with the range (minimum–maximum) displayed. We conducted the Kolmogorov-Smirnov goodness-of-fit test to analyze the normality of the continuous variables. We employed one-way ANOVA to assess the variables that were f...
38648458_p4
38648458
Statistical analysis
3.99223
biomedical
Study
[ 0.9994131326675415, 0.00027157639851793647, 0.00031539666815660894 ]
[ 0.9989683628082275, 0.0006347322487272322, 0.0003393446677364409, 0.00005750438867835328 ]
en
0.999997
In the control group (group 1), we collected control conjunctiva from 30 patients (16 females and 14 males) with an age range of 48–70 years (mean: 56.66 ± 7.10 years). We collected primary pterygia from 30 patients (17 females and 13 males) aged 48–72 years (mean: 59.13 ± 7.43 years) (group 2) and collected recurrent ...
38648458_p5
38648458
Results
3.959591
biomedical
Study
[ 0.9985363483428955, 0.0012081707827746868, 0.00025547537370584905 ]
[ 0.9991298317909241, 0.0005490658804774284, 0.0001739892759360373, 0.0001471523428335786 ]
en
0.999997
We did, however, find significant differences in the percentage of CD44, E-cadherin, and PCNA between the groups ( P < 0.001, P < 0.001, and P < 0.001, respectively). We observed that the CD44 and PCNA percentages were statistically higher ( P < 0.001 and P < 0.001, respectively) in experimental groups 2 and 3 than in ...
38648458_p6
38648458
Results
4.076971
biomedical
Study
[ 0.9993122816085815, 0.0003409051860217005, 0.00034674888593144715 ]
[ 0.9995984435081482, 0.00015372135385405272, 0.00020302929624449462, 0.00004481455107452348 ]
en
0.999997
This study examined the levels of PCNA, CD44, and E-cadherin in pterygium tissue samples and control conjunctiva. Our results indicated a significant increase in positive PCNA and CD44 rates in the pterygium samples, while the E-cadherin level was significantly lower than in control conjunctival tissue samples. These f...
38648458_p7
38648458
Discussion
4.109038
biomedical
Study
[ 0.9995229244232178, 0.00029847354744561017, 0.00017865416884887964 ]
[ 0.9994076490402222, 0.00017646842752583325, 0.0003426434413995594, 0.0000732537591829896 ]
en
0.999996
Despite being considered benign, pterygium exhibits properties similar to metastatic tissues, including dense cell proliferation, inflammation, cell migration, local angiogenesis, and epithelial-mesenchymal transition.[ 18 19 20 ] Our research also revealed a lower amount of E-cadherin in pterygium tissues than in cont...
38648458_p8
38648458
Discussion
4.118536
biomedical
Study
[ 0.9995905756950378, 0.000209882011404261, 0.000199573376448825 ]
[ 0.9993045330047607, 0.00016303625307045877, 0.00047313678078353405, 0.00005920152034377679 ]
en
0.999995
PCNA is a protein associated with cell proliferation and is used as a tumor marker for some types of cancer.[ 24 25 26 ] Our study found that PCNA expression was significantly higher in pterygium samples than in control conjunctiva samples. Cell proliferation plays a vital role in the pathogenesis of pterygium. In the ...
38648458_p9
38648458
Discussion
4.092379
biomedical
Study
[ 0.9995598196983337, 0.00022220467508304864, 0.00021791910694446415 ]
[ 0.9994282126426697, 0.0001691827637841925, 0.0003444436297286302, 0.000058105579228140414 ]
en
0.999997
Similarly, CD44 is a protein that regulates cellular processes such as cell division, adhesion, and migration. Aside from its usual cellular roles, CD44 has been identified as a significant indicator of the presence of cancer stem cells.[ 30 31 ] Studies have shown that CD44 contributes to tumor invasion and metastasis...
38648458_p10
38648458
Discussion
4.064418
biomedical
Study
[ 0.9996228218078613, 0.00014016253408044577, 0.00023698678705841303 ]
[ 0.9404755234718323, 0.0030639234464615583, 0.05622682720422745, 0.00023377453908324242 ]
en
0.999998
The present study had some limitations. As it was not considered ethically appropriate to take conjunctival samples from healthy eyes, we took the control group from the superior bulbar conjunctiva of eyes with pterygium. Another limitation is the small number of cases.
38648458_p11
38648458
Discussion
2.158044
biomedical
Study
[ 0.9973725080490112, 0.0010145740816369653, 0.001612975262105465 ]
[ 0.988655686378479, 0.010243975557386875, 0.0005375186447054148, 0.0005627814680337906 ]
en
0.999997
In conclusion, our research provides valuable insights into the pathogenesis of pterygium and the roles of PCNA, CD44, and E-cadherin in this disease. These findings can potentially be used to develop new therapeutic strategies to treat pterygium and prevent relapses.
38648458_p12
38648458
Conclusion
3.924962
biomedical
Study
[ 0.9997463822364807, 0.00012322365364525467, 0.0001303449971601367 ]
[ 0.996160626411438, 0.0014329425757750869, 0.0022682934068143368, 0.00013815844431519508 ]
en
0.999997
The study was supported by the Scientific Investigations Foundation of Balikesir University . The research adhered to the ethical principles stated in the Declaration of Helsinki. Prior to commencing the study, the necessary approval was sought and obtained from the Clinical Research Ethics Committee of Balıkesir Unive...
38648458_p13
38648458
Financial support and sponsorship
0.983706
biomedical
Other
[ 0.5418579578399658, 0.006068243645131588, 0.45207375288009644 ]
[ 0.011190895922482014, 0.9875049591064453, 0.0006296118372119963, 0.0006745069986209273 ]
en
0.999998
There are no conflicts of interest.
38648458_p14
38648458
Conflicts of interest
0.935876
other
Other
[ 0.02788134478032589, 0.002627168782055378, 0.9694914221763611 ]
[ 0.007451080251485109, 0.9894022941589355, 0.0018082356546074152, 0.0013383158948272467 ]
en
0.999995
A case-control study was conducted on 17 eyes with primary pterygium in patients above 10 years of age attending the cornea services at the tertiary care ophthalmic service in New Delhi. The following grading system was used:
38324633_p0
38324633
Methods
2.896642
biomedical
Study
[ 0.9942044615745544, 0.0052086105570197105, 0.0005868805455975235 ]
[ 0.9932698607444763, 0.004717385396361351, 0.00033605360658839345, 0.001676710438914597 ]
en
0.999997
Grade 0: Pingeculum, posterior to the limbus Grade 1: Tissue involvement to the limbus Grade 2: Tissue just onto the limbus Grade 3: Tissue between the limbus and the pupillary margin Grade 4: Tissue central to the pupillary margin.
38324633_p1
38324633
Methods
2.978579
biomedical
Other
[ 0.9130558967590332, 0.08232302218675613, 0.004621122498065233 ]
[ 0.006501033436506987, 0.984086811542511, 0.0004447824030648917, 0.008967375382781029 ]
en
0.999998
Cases of recurrent pterygium were excluded from the study. The study period was 12 months. Approval from the institutional ethics committee was taken before starting the study. The study was registered with the Trial Registry of India and followed the tenets of the Declaration of Helsinki. Informed consent was obtained...
38324633_p2
38324633
Methods
2.00104
biomedical
Study
[ 0.9802238941192627, 0.017534073442220688, 0.002242042450234294 ]
[ 0.9142971038818359, 0.08123651891946793, 0.0010893322760239244, 0.003377071348950267 ]
en
0.999998
A comprehensive ophthalmological workup of both eyes was done, which included a detailed slit-lamp examination to measure the size of pterygium (length and width), conjunctival, and corneal vascularization. Assessment of visual acuity, measurement of the thickness of pterygium over cornea and conjunctiva by AS-OCT (ant...
38324633_p3
38324633
Methods
3.967088
biomedical
Study
[ 0.9626088738441467, 0.03713863343000412, 0.00025246833683922887 ]
[ 0.976593017578125, 0.013775557279586792, 0.001942471251823008, 0.007689015008509159 ]
en
0.999998
All cases were primary pterygium cases in the nasal quadrant. The majority of the samples were grades 2–3 as per the grading system mentioned before. Pterygium excision with the limbal conjunctival autograft technique was performed under peribulbar anesthesia by the same surgeon.
38324633_p4
38324633
Procedure
3.053241
biomedical
Study
[ 0.9511833786964417, 0.047935184091329575, 0.0008814252214506269 ]
[ 0.5933809876441956, 0.34048566222190857, 0.0023606044705957174, 0.06377271562814713 ]
en
0.999999
Approximately 0.5 mL of saline was injected under the belly of the pterygium by using a 26-G needle mounted on a 2-mL syringe. The head of the pterygium was avulsed from its attachment at the cornea by reverse stripping by using slow and deliberate traction holding its free end parallel to the cornea. The fibrovascular...
38324633_p5
38324633
Procedure
3.981452
biomedical
Other
[ 0.9775704145431519, 0.021836671978235245, 0.0005929727340117097 ]
[ 0.40361490845680237, 0.5695657730102539, 0.0036205253563821316, 0.023198768496513367 ]
en
0.999999
Donor conjunctiva harvested from the superotemporal quadrant of the same eye was 4 mm × 4 mm larger than the size of the bare sclera; 3 mm × 3 mm of this donor conjunctiva was sent for IHC to serve as control. The dissection was continued at the donor site for approximately 1 mm into the peripheral cornea, beyond the v...
38324633_p6
38324633
Procedure
3.977307
biomedical
Other
[ 0.7658726572990417, 0.23270255327224731, 0.0014246912905946374 ]
[ 0.33178484439849854, 0.4146499037742615, 0.0044423178769648075, 0.24912293255329132 ]
en
0.999997
Samples were fixed in 10% formalin, processed, and embedded in paraffin. All samples were sectioned at 3-μm thickness and mounted on positively charged glass slides labeled with barcodes after sectioning and fixed overnight at 37°C for immunohistochemical (IHC) staining. After deparaffinization in alcohol, samples were...
38324633_p7
38324633
Immunohistochemistry
4.041426
biomedical
Study
[ 0.999053418636322, 0.0007263545994646847, 0.00022022910707164556 ]
[ 0.8819519877433777, 0.11480114609003067, 0.0021419539116322994, 0.0011049044551327825 ]
en
0.999995
IHC staining for p53, Bcl-2, VEGF, and Ki-67 was performed on 17 primary pterygium (cases) and 17 healthy conjunctiva tissue samples (controls) from the same eye. IHC using the streptavidin–biotin peroxidase method was performed on paraffin-embedded tissues, and antigen retrieval was achieved by heat retrieval methods ...
38324633_p8
38324633
Immunohistochemistry
4.083071
biomedical
Study
[ 0.9995428323745728, 0.0003016961563844234, 0.00015552605327684432 ]
[ 0.9990480542182922, 0.0006046670605428517, 0.0002582762099336833, 0.00008893889025785029 ]
en
0.999998
Samples were incubated with the following antibodies for 60 min at 25°C. The primary antibodies used were as follows:
38324633_p9
38324633
Immunohistochemistry
2.675905
biomedical
Study
[ 0.9962303042411804, 0.0006009761709719896, 0.0031687330920249224 ]
[ 0.5886800289154053, 0.4070042073726654, 0.0030280782375484705, 0.0012876549735665321 ]
en
0.999997
p53 Mouse Monoclonal Antibody dilution 1:100 (Clone BP-53-12, (PathnSitu, Livermore, CA, USA) Ki-67 (Clone: MIB1) Mouse Monoclonal Antibody dilution 1:100 (PathnSitu, Pleasanton, CA, USA) Bcl-2 alpha AB1; (clone 100/D5, Thermo Scientific, Kalamazoo, USA) VEGF RTU (ready to use),7 mL, (Clone RBT, BIOSB, SantaBarbara, CA...
38324633_p10
38324633
Immunohistochemistry
1.97273
biomedical
Other
[ 0.9923911690711975, 0.0008997751283459365, 0.00670907087624073 ]
[ 0.04433302581310272, 0.9540458917617798, 0.0007996298372745514, 0.0008214410627260804 ]
en
0.85714
A light microscope (OLYMPUS BX53) was used, and the immune-positive cells were examined manually. Staining intensity was graded in the following manner for all the biomarkers: 0 –negative, 1+ – weak, 2+ – intermediate, and 3+ – strong staining.
38324633_p11
38324633
Immunohistochemistry
3.614457
biomedical
Study
[ 0.9992444515228271, 0.00038454358582384884, 0.00037109950790181756 ]
[ 0.9769739508628845, 0.02215193212032318, 0.000565499474760145, 0.0003085523785557598 ]
en
0.999998
p53 positivity: The basal epithelial cells were counted. At least 100 cells of tissue (including immune-positive cells) in three microscopic fields (magnification 100×) from the sample were studied. The expression of the p53 protein was marked positive if it was present in more than 10% of tissue cells and negative if ...
38324633_p12
38324633
Immunohistochemistry
4.130123
biomedical
Study
[ 0.9994016885757446, 0.0003697590436786413, 0.00022856007853988558 ]
[ 0.999122679233551, 0.0004014275036752224, 0.00041193983634002507, 0.0000639051286270842 ]
en
0.999994
The Statistical Package for Social Sciences (SPSS) PC 21version was used for data analysis. Fisher’s exact test was used to calculate the association and its significance between categorical variables, for example, case/control and positivity. Because the sample size was small, the P value was considered significant at...
38324633_p13
38324633
Statistical analysis
3.447607
biomedical
Study
[ 0.9987449645996094, 0.00025486215599812567, 0.0010000421898439527 ]
[ 0.9973875880241394, 0.0022920549381524324, 0.0002498042013030499, 0.00007053319131955504 ]
en
0.999996
The immunohistochemistry of the samples and controls revealed p53 positivity (The expression of p53 protein was marked positive if it was present in more than 10% of tissue cells and negative if present in less than 10% cells.) in 47.05% (8 out of 17) pterygium samples [ Fig. 1 a] and 29.4% (5 out of 17) controls [ Fig...
38324633_p14
38324633
Result
4.096953
biomedical
Study
[ 0.9994304776191711, 0.00037559628253802657, 0.0001938438945217058 ]
[ 0.9994576573371887, 0.0002499891852494329, 0.00021252250007819384, 0.00007978802750585601 ]
en
0.999997
Nine out of 17 (52.9%) cases [ Fig. 2 a] and control samples [ Fig. 2 b] were positive for Ki-67 expression. The difference in the staining pattern between the two groups was not statistically significant ( P < 1.000) [ Table 2 ].
38324633_p15
38324633
Result
3.890316
biomedical
Study
[ 0.9989418387413025, 0.0007122202077880502, 0.00034595123725011945 ]
[ 0.9994192123413086, 0.00031901145121082664, 0.00016798140131868422, 0.00009377621609019116 ]
en
0.999994
Bcl-2 positivity was seen in 10 out of 17 pterygium samples (58.8%) [ Fig. 3 a] and 12 out of 17 controls (70.5%) [ Fig. 3 b], with no statistical difference between the two groups ( P < 0.455) [ Table 3 ].
38324633_p16
38324633
Result
4.045304
biomedical
Study
[ 0.9992470741271973, 0.000477752648293972, 0.0002751483698375523 ]
[ 0.999542236328125, 0.00021837587701156735, 0.00017144688172265887, 0.00006794976798119023 ]
en
0.999996
VEGF expression was seen in both epithelial [ Table 4 a] and endothelial cells [ Table 4 b] of the samples [ Fig. 4 a] and controls [ Fig. 4 b], with no statistical difference between the two groups, with P = 1.000 for the epithelial staining and P = 0.637 for endothelial staining.
38324633_p17
38324633
Result
4.020721
biomedical
Study
[ 0.9993027448654175, 0.0003742444096133113, 0.00032293712138198316 ]
[ 0.9995125532150269, 0.0002631198149174452, 0.00016797818534541875, 0.00005637258436763659 ]
en
0.999997
Pterygium is regarded as a degenerative condition with multifaceted pathology that displays apparent conflicting characteristics. It is considered benign because of its self-limiting and superficial nature; however, because of its highly proliferative and potentially recurrent nature, it has been said to possess signs ...
38324633_p18
38324633
Discussion
3.58247
biomedical
Other
[ 0.9988918900489807, 0.0004006009839940816, 0.0007075577741488814 ]
[ 0.21911467611789703, 0.6611925363540649, 0.11791133880615234, 0.0017813906306400895 ]
en
0.999997
Various opinions regarding the mechanism of pterygium development have been discussed in the literature. However, the exact pathogenesis is still a dilemma. Chronic inflammation occurring in the limbal conjunctival vessels or conjunctival epithelium is thought to be one of the factors in the pathogenesis.
38324633_p19
38324633
Discussion
2.510327
biomedical
Other
[ 0.9975243210792542, 0.0009538338636048138, 0.0015218771295621991 ]
[ 0.1066930890083313, 0.8423051834106445, 0.04747229069471359, 0.003529394045472145 ]
en
0.999996
Pathophysiology of pterygium due to exposure to UV rays is perhaps the most accepted risk factor for the occurrence of pterygium.[ 2 18 ] There is a void in the existing literature regarding the pathophysiology of pterygium and its overlap with other malignant conditions (ocular surface squamous neoplasia and skin canc...
38324633_p20
38324633
Discussion
4.001457
biomedical
Study
[ 0.9995939135551453, 0.0001636369852349162, 0.00024250468413811177 ]
[ 0.8971537947654724, 0.018899738788604736, 0.0834423154592514, 0.0005041453987360001 ]
en
0.999997
Various inflammatory and apoptotic biomarkers have been studied in the literature to help understand the molecular level of the occurrence and recurrence of pterygium. A study of these biomarkers can help with targeted therapy in pterygium patients and prevent recurrence.[ 20 21 ]
38324633_p21
38324633
Discussion
2.699521
biomedical
Other
[ 0.998552143573761, 0.00043669360456988215, 0.0010111198062077165 ]
[ 0.2602882385253906, 0.6906720399856567, 0.04705839976668358, 0.001981321955099702 ]
en
0.999996
The purpose of the current study was to ascertain p53, Ki-67, Bcl-2, and VEGF expression in pterygium and healthy conjunctiva of the same eye and if this could further help in the development of targeted therapy. The ethical clearance for the study of biomarkers from the conjunctiva of the normal/healthy eye was refuse...
38324633_p22
38324633
Discussion
3.781578
biomedical
Study
[ 0.9992038607597351, 0.00048398005310446024, 0.00031223794212564826 ]
[ 0.9991025924682617, 0.0006659184000454843, 0.0001278143172385171, 0.0001037256806739606 ]
en
0.999997
Spandidos et al . in their study concluded that there is a role for tumor suppressor genes and decreased fidelity in DNA replication and repair in the development of pterygium.
38324633_p23
38324633
Discussion
3.718208
biomedical
Study
[ 0.9995489716529846, 0.00012561542098410428, 0.00032546260626986623 ]
[ 0.9917478561401367, 0.00447853421792388, 0.0035753126721829176, 0.00019832770340144634 ]
en
0.999997
p53 is a tumor suppressor gene that controls the cell cycle and is involved in DNA repair and synthesis, cell differentiation, and apoptosis. The p53 protein can be identified by IHC staining by using a monoclonal antibody against it. Normal cells are negative for the stain as the protein concentration is very low due ...
38324633_p24
38324633
Discussion
4.025587
biomedical
Study
[ 0.9993382096290588, 0.00029429575079120696, 0.0003675316693261266 ]
[ 0.6637881398200989, 0.3159632980823517, 0.019327960908412933, 0.0009206125978380442 ]
en
0.999995
Tan et al. performed IHC staining on eight pterygia specimens and found three of them (37.5%) to be positive for abnormal expression of p53. Dushku and Reid found increased nuclear p53 in the limbal epithelium of pterygia, limbal tumors, and pinguecula and suggested that the existence of p53 mutation in the cells is an...
38324633_p25
38324633
Discussion
4.051946
biomedical
Study
[ 0.9995434880256653, 0.00020779651822522283, 0.000248730240855366 ]
[ 0.9809762239456177, 0.00047951971646398306, 0.018389571458101273, 0.00015478554996661842 ]
en
0.999998
Our study observed a higher p53 expression in primary pterygium at 47.05% as compared to 29.4% positivity in normal conjunctiva [ Table 1 ], with the majority of cells in both groups showing 3+ positivity; however, it was not statistically significant, which is in concurrence with the above review of the literature.
38324633_p26
38324633
Discussion
3.947397
biomedical
Study
[ 0.9995915293693542, 0.00023664017498958856, 0.00017182838928420097 ]
[ 0.9967818260192871, 0.0003028713690582663, 0.0028057105373591185, 0.00010955056495731696 ]
en
0.999999
Ki-67 plays a role in all of the active phases (G1, S, G2, and M) of the cell cycle; it is not related to the resting cells (G0). Thus, the proliferative cellular activity can be evaluated by the detection of Ki-67 nuclear protein.[ 26 27 ]
38324633_p27
38324633
Discussion
3.522061
biomedical
Study
[ 0.9979134202003479, 0.00026518653612583876, 0.0018213726580142975 ]
[ 0.5732685327529907, 0.4224320948123932, 0.003753571305423975, 0.0005457961815409362 ]
en
0.999997
Mahesh et al . studied the correlation between p53 and Ki-67 expression with the severity and duration of the pterygium on 43 patients and found that the expression of cell proliferation marker Ki-67 increases with the duration of pterygium and p53 expression increases with the duration and severity of pterygium. A stu...
38324633_p28
38324633
Discussion
3.955107
biomedical
Study
[ 0.9995853304862976, 0.00015651398280169815, 0.0002581943990662694 ]
[ 0.994795024394989, 0.00032409789855591953, 0.004795151297003031, 0.00008570215140935034 ]
en
0.999997
In our study, both pterygium and control in our study showed weak staining on Ki-67 in equal numbers (52.9%) in each group. A greater number of cases with >5% positivity of Ki-67 index in pterygium was observed compared to controls. The correlation between p53 and Ki-67 was not studied by us.
38324633_p29
38324633
Discussion
3.207239
biomedical
Study
[ 0.9990547299385071, 0.00039203750202432275, 0.0005531686474569142 ]
[ 0.9985860586166382, 0.0011252835392951965, 0.00017141833086498082, 0.00011726673983503133 ]
en
0.999998
Bcl-2 belongs to the family of apoptosis regulatory proteins, which can induce or inhibit apoptosis. Bcl-2 is primarily expressed in the basal epithelial layer of all pterygium epithelial cells and normal conjunctiva as well. However, the ratio of Bcl-2 to Bcl-associated X protein (BAX, an apoptosis regulatory protein)...
38324633_p30
38324633
Discussion
3.902771
biomedical
Study
[ 0.999054491519928, 0.0002334209711989388, 0.0007120689260773361 ]
[ 0.542667806148529, 0.4504050612449646, 0.006253289058804512, 0.0006738352822139859 ]
en
0.999995
There was higher expression of Bcl-2 in normal conjunctiva (70.5%) compared to pterygium (58.8%), which was not statistically significant ( P < 0.4). However, BAX, which is the apoptosis regulatory protein, has not been evaluated in our study, and hence the ratio of Bcl-2 to BAX cannot be commented upon.
38324633_p31
38324633
Discussion
3.808635
biomedical
Study
[ 0.9993740916252136, 0.0002602411259431392, 0.0003658026980701834 ]
[ 0.9993614554405212, 0.00043992497376166284, 0.00014245245256461203, 0.000056089200370479375 ]
en
0.999998
Turan M and Turan showed significantly increased p53, Bcl-2, and Ki-67 expression in primary and recurrent pterygiums when compared to normal conjunctiva of the eye, with no disease process in play ( P < 0.001). Suren E et al . demonstrated an increased expression of p53, Bcl-2, and Ki-67 levels in pterygium samples co...
38324633_p32
38324633
Discussion
4.004879
biomedical
Study
[ 0.9996993541717529, 0.00013886956730857491, 0.00016180577222257853 ]
[ 0.9969062209129333, 0.0004073904419783503, 0.00261230138130486, 0.00007408857345581055 ]
en
0.999997
Our study, however, was not granted ethical clearance for the use of conjunctiva of the healthy eye without any disease process for control samples.
38324633_p33
38324633
Discussion
1.868475
biomedical
Study
[ 0.9939243197441101, 0.001390518737025559, 0.0046851481311023235 ]
[ 0.7978847622871399, 0.1983024924993515, 0.00188981625251472, 0.0019228652818128467 ]
en
0.999998
VEGF plays an important role in the angiogenesis and neovascularization in the pathogenesis of pterygium. It has been demonstrated on IHC in the epithelial and endothelial cells of pterygium samples by Bianchi et al . They reported a statistically significant difference in staining pattern among pterygium and normal co...
38324633_p34
38324633
Discussion
4.101744
biomedical
Study
[ 0.9996637105941772, 0.0001731606462271884, 0.00016309063357766718 ]
[ 0.9992469549179077, 0.0002746678073890507, 0.0004203709540888667, 0.000058035839174408466 ]
en
0.999998
The study had assumed that it was impossible to use control specimens from normal conjunctiva adjacent to pterygium as it may also have potential abnormalities, which was evident in our study where VEGF expression was observed in all of the pterygium (100%) and 82.3% of control samples.
38324633_p35
38324633
Discussion
3.326681
biomedical
Study
[ 0.9991211295127869, 0.0002850664022844285, 0.0005938155809417367 ]
[ 0.9981196522712708, 0.0015935112023726106, 0.00016799865989014506, 0.00011884020932484418 ]
en
0.999997
Aspiotis M et al . concluded that normal conjunctiva shows intense expression of VEGF in epithelial cells. However, this is not capable of inducing angiogenesis because of low corresponding mean vascular density (MVD) values. Our study showed VEGF expression exclusively in the epithelial cells in 23.5% of the control s...
38324633_p36
38324633
Discussion
4.01332
biomedical
Study
[ 0.9995629191398621, 0.00018036160327028483, 0.0002566738403402269 ]
[ 0.9993813037872314, 0.0002763733209576458, 0.0002896420191973448, 0.00005267448796075769 ]
en
0.999998
There is a lot of variability in biomarkers expression of pterygium in various studies as controls used were variable in different studies. Most of the reviewed literature either did not have a control group or it was too small when compared to the pterygium group or was taken from the healthy eyes of other or the same...
38324633_p37
38324633
Conclusion
2.357971
biomedical
Review
[ 0.9961452484130859, 0.0009069814695976675, 0.0029477716889232397 ]
[ 0.251140832901001, 0.1415484994649887, 0.6043218374252319, 0.0029888590797781944 ]
en
0.999995
Our study differs in that the control group was taken from the apparently healthy quadrant of the affected eye, with the expression of biomarkers from pterygium and healthy unaffected conjunctiva being comparable. This leads us to conclude that pterygium, against common belief, might not be a localized disease process ...
38324633_p38
38324633
Conclusion
4.051267
biomedical
Study
[ 0.9994250535964966, 0.00026259993319399655, 0.00031233715708367527 ]
[ 0.9995054006576538, 0.00021951747476123273, 0.00022525229724124074, 0.000049826241593109444 ]
en
0.999996
Self funded.
38324633_p39
38324633
Financial support and sponsorship
0.992349
other
Other
[ 0.009668522514402866, 0.0019550155848264694, 0.9883764386177063 ]
[ 0.0038548647426068783, 0.9937599301338196, 0.0014697116566821933, 0.0009154888102784753 ]
en
0.571427
There are no conflicts of interest.
38324633_p40
38324633
Conflicts of interest
0.935876
other
Other
[ 0.02788134478032589, 0.00262717274017632, 0.9694914221763611 ]
[ 0.007451090961694717, 0.9894022941589355, 0.0018082363530993462, 0.0013383196201175451 ]
en
0.999996
Patients with AIDS were HAART-naïve inpatients from the Infectious Disease Center of Beijing You’an Hospital, who routinely underwent ophthalmic consultations to screen for ocular lesions during their stay and provided consent for their relevant medical records to be reviewed to determine their suitability for particip...
38317305_p0
38317305
Subjects
4.022411
biomedical
Study
[ 0.9983441829681396, 0.0014255051501095295, 0.0002302799839526415 ]
[ 0.9990912675857544, 0.00046828450285829604, 0.0003000449505634606, 0.00014046658179722726 ]
en
0.999997
A total of 154 participants were eventually enrolled in this study, with an estimated 80 patients with AIDS and 74 healthy controls, far greater than the sample size requirements for the desired study power, calculated by G*Power (version 3·1·9·7; Erdfelder, Faul, & Buchner, Düsseldorf, Germany), with noninvasive tear ...
38317305_p1
38317305
Subjects
4.132968
biomedical
Study
[ 0.9986749291419983, 0.0010721392463892698, 0.0002528861223254353 ]
[ 0.9983664155006409, 0.0012875546235591173, 0.00022737050312571228, 0.00011867589637404308 ]
en
0.999998
All participants underwent a comprehensive ocular surface assessment performed by the same ophthalmologist (Dr. F. R. and Dr. Q. F.), following the principle of invasiveness from small to large, to minimize the impact on tear film physiology. Among the ocular indicators observed, NIBUT and meibomian gland loss (MGL) we...
38317305_p2
38317305
Measurements
4.128529
biomedical
Study
[ 0.9981842637062073, 0.0016827950021252036, 0.00013289431808516383 ]
[ 0.9980520009994507, 0.0011264340719208121, 0.0005459641688503325, 0.00027554677217267454 ]
en
0.999996
DED symptoms were assessed using the Ocular Surface Disease Index (OSDI) questionnaire on a scale of 0–100, which consisted of 12 questions. Questions 1–5 were on symptoms of eye discomfort, questions 6–9 were on limitations of daily activities, and questions 10–12 were on environmental triggers, with higher scores rep...
38317305_p3
38317305
DED symptomology evaluation
3.879151
biomedical
Study
[ 0.998853325843811, 0.0009266271372325718, 0.0002200011076638475 ]
[ 0.9914205074310303, 0.007467632181942463, 0.0008661547326482832, 0.00024571517133153975 ]
en
0.999998
Tear film LLT was measured quantitatively using LipiView with a detection time of 20 s, during which the patient blinked normally while fixating on an internal fixation target consisting of light-emitting diodes. The outcomes were represented in interferometric color units (ICU), with one ICU representing 1 nm; there w...
38317305_p4
38317305
Tear film and blinking pattern evaluation
4.218011
biomedical
Study
[ 0.999025821685791, 0.0008089813054539263, 0.0001651807251619175 ]
[ 0.9979885816574097, 0.0009831266943365335, 0.0008426934946328402, 0.00018566433573141694 ]
en
0.999997
Vital corneal fluorescein staining (CFS) scores were obtained using sodium fluorescein in the inferior fornix to determine the degree of corneal epitheliopathy. The score was based on the National Eye Institute/Industry grading scale, which divided the cornea into five sections; each section was graded from 0 (absent) ...
38317305_p5
38317305
Ocular surface damage evaluation
4.083632
biomedical
Study
[ 0.9988786578178406, 0.0009287828579545021, 0.00019258479005657136 ]
[ 0.9949947595596313, 0.004205471836030483, 0.0005282389465719461, 0.00027152718394063413 ]
en
1
MGL of the upper and lower eyelids was graded by infrared meibography using K5M in the Meibo-Scan enhanced contrast according to the criteria of Arita et al ., with the eyelids turned over. The grades denoted the following: grade 0, no loss of meibomian glands; grade 1, the loss was less than one-third of the total mei...
38317305_p6
38317305
MGL evaluation
4.074556
biomedical
Study
[ 0.9990686774253845, 0.0007488614646717906, 0.00018245101091451943 ]
[ 0.9968863129615784, 0.0020507799927145243, 0.0008567440672777593, 0.00020620436407625675 ]
en
0.999997
The diagnostic criteria for DED were based on the TFOS DEWS II Diagnostic Methodology report; if the OSDI score was ≥13 and NIBUT was <10 s or CFS showed >5 corneal spots, a dry eye diagnosis could be made.
38317305_p7
38317305
DED diagnosis
3.763973
biomedical
Study
[ 0.99524986743927, 0.004491002298891544, 0.00025912094861268997 ]
[ 0.5780421495437622, 0.4116010069847107, 0.005211051553487778, 0.005145789589732885 ]
en
0.999999
Statistical analyses were performed using IBM Statistical Package for the Social Sciences (SPSS) Statistics software (version 26·0; New York, NY, USA). The measurement data were tested for normality using the Shapiro–Wilk test, and the Mann–Whitney U test was used for non-normally distributed data, presented as median ...
38317305_p8
38317305
Statistical analysis
3.935707
biomedical
Study
[ 0.9994906187057495, 0.00023474245972465724, 0.0002746849786490202 ]
[ 0.9988681077957153, 0.0006982985069043934, 0.00037309309118427336, 0.000060541544371517375 ]
en
0.999997
This cross-sectional, observational, case–control study adhered to the tenets of the Declaration of Helsinki. It was approved by the Institutional Ethics Committee of Beijing You’an Hospital, Capital Medical University and is registered with the Chinese Clinical Trial Registry . Written informed consent was obtained fr...
38317305_p9
38317305
Ethics approval
2.752542
biomedical
Study
[ 0.9982125759124756, 0.0011148025514557958, 0.0006727073341608047 ]
[ 0.9925625920295715, 0.006368371192365885, 0.0006346383597701788, 0.00043436113628558815 ]
en
0.999998
The subjects in both groups were Chinese men. The demographic and clinical data of all participants, comprising 80 AIDS patients and 74 healthy controls, are summarized in Table 1 . Ocular clinical measurements are presented in Table 2 . Tear film tests revealed a shorter NIBUT (median 3.76 vs. 8.54 s), thinner LLT (me...
38317305_p10
38317305
Results
4.125648
biomedical
Study
[ 0.9990924596786499, 0.0006992794806137681, 0.00020834094902966172 ]
[ 0.9993361830711365, 0.00017768025281839073, 0.00038539653178304434, 0.00010077744809677824 ]
en
0.999998
Correlations between the CD4+ T-cell count and blood viral load in patients with AIDS and ocular findings are shown in Table 3 . Significant negative correlations between the blood viral load and OSDI score ( r = -3.50, P = 0.027) and Schirmer I test ( r = -0.374, P = 0.017) were detected in patients with AIDS. No sign...
38317305_p11
38317305
Results
4.064007
biomedical
Study
[ 0.9993593096733093, 0.0004329615621827543, 0.0002077639801427722 ]
[ 0.9993510842323303, 0.00016509542183484882, 0.0004135890631005168, 0.00007024723890936002 ]
en
0.999997
In this case–control study, we conducted a novel and comprehensive evaluation of the ocular surface manifestations of patients with HAART-naïve AIDS and HIV-negative individuals, particularly those with DED. The tear film status was altered in patients with AIDS, even without significant ocular symptoms. Notably, these...
38317305_p12
38317305
Discussion
4.045279
biomedical
Study
[ 0.999350368976593, 0.0005218956503085792, 0.00012773634807672352 ]
[ 0.999202311038971, 0.0002865683345589787, 0.0003876931150443852, 0.0001234030060004443 ]
en
0.999996
A stable tear film is the cornerstone of ocular surface health, forming the primary refractive surface for light entering the visual system and protecting and nourishing the corneal tissue. Since the 2017 TFOS DEWS II highlighted the loss of tear film homeostasis as the most central pathophysiological alteration in DED...
38317305_p13
38317305
Discussion
4.149076
biomedical
Study
[ 0.9995741248130798, 0.0002756595495156944, 0.00015027995686978102 ]
[ 0.9988510608673096, 0.00034488944220356643, 0.0007196213700808585, 0.00008437351061729714 ]
en
0.999996
Abnormal tear production and Sjögren syndrome (SS)-like illness in patients with HIV/AIDS have been reported in many studies. Indeed, this study found a significant decrease in Schirmer I test values as an evidence of aqueous deficiency in patients with AIDS. It is well known that the bulk of the tear film volume is se...
38317305_p14
38317305
Discussion
4.269575
biomedical
Study
[ 0.9995056390762329, 0.0003232005110476166, 0.0001710452197585255 ]
[ 0.9986490607261658, 0.00023736589355394244, 0.0010072804288938642, 0.00010631961049512029 ]
en
0.999996
The LipiView interferometer measurements also revealed mild thinning of the tear film LLT in AIDS patients. Nevertheless, the upper detection limit of the machine was only 100 nm; thus, we suspected that if the measurement range was wider, the difference in LLT between the two groups would be more pronounced. The optim...
38317305_p15
38317305
Discussion
4.124642
biomedical
Study
[ 0.9995637536048889, 0.00026293762493878603, 0.00017327266687061638 ]
[ 0.999299168586731, 0.00022656902729067951, 0.0004046785179525614, 0.00006959724851185456 ]
en
0.999998
Given that the tear film lipid layer is mainly composed of meibum secreted by the meibomian gland and distributed on the tear film during each blink, we further analyzed the blinking pattern and meibomian gland dropout in patients with AIDS using interferometry and meibography. We observed a greater loss of the upper, ...
38317305_p16
38317305
Discussion
4.150081
biomedical
Study
[ 0.999534010887146, 0.00032174642547033727, 0.00014432378520723432 ]
[ 0.9991305470466614, 0.0002174365654354915, 0.0005640396848320961, 0.00008789773710304871 ]
en
0.999998
We accurately reported 37.5% prevalence of DED in patients with AIDS based on the TFOS DEWS II dry eye diagnostic criteria; however, we were surprised that there was no statistically significant difference in this value compared to sex-, age-, and ethnicity-matched healthy controls, unlike what had been reported in pre...
38317305_p17
38317305
Discussion
4.167541
biomedical
Study
[ 0.9993140697479248, 0.0005448381416499615, 0.00014107539027463645 ]
[ 0.9980066418647766, 0.0003523582126945257, 0.0015038072597235441, 0.00013716820103581995 ]
en
0.999996
Data in this research were collected from March 25, 2022 to July 20, 2022, when severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) broke out nationwide and intermittently surged regionally in China. During the COVID-19 pandemic, the relevant stay-at-home measures, the consequent lifestyle changes, and eye fat...
38317305_p18
38317305
Discussion
4.060592
biomedical
Study
[ 0.9993957281112671, 0.00033777827047742903, 0.0002665080246515572 ]
[ 0.9995089769363403, 0.0002016756043303758, 0.00022699381224811077, 0.00006239413050934672 ]
en
0.999996
Several limitations also need to be mentioned, including this study’s single-center design, small sample size, and only male features. Furthermore, the case–control design excluded ocular signs and symptoms after HAART and those outside the hospital. Studies focusing on tear osmolarity, corneal perception, and meibum q...
38317305_p19
38317305
Discussion
4.120809
biomedical
Study
[ 0.9993497729301453, 0.0005246719811111689, 0.00012561518815346062 ]
[ 0.9983661770820618, 0.0003282519755885005, 0.0011725182412192225, 0.00013301591388881207 ]
en
0.999996
Even in the absence of other ocular symptoms, patients with AIDS might be predisposed to have an altered tear film status, that is, poorer tear film stability, a lower tear secretion volume, a thinner tear film lipid layer, and more severe meibomian gland dropout. As the AIDS epidemic continues to spread worldwide, esp...
38317305_p20
38317305
Conclusion
3.88847
biomedical
Study
[ 0.9993569254875183, 0.00042594250408001244, 0.00021720249787904322 ]
[ 0.6258431673049927, 0.04530426114797592, 0.32775798439979553, 0.0010946112452074885 ]
en
0.999999
This study was supported by the Key Project of Tianjin Eye Hospital , and the Tianjin Key Medical Discipline (Specialty) Construction Project (TJYXZDXK-016A).
38317305_p21
38317305
Financial support and sponsorship
0.958059
other
Other
[ 0.03468509018421173, 0.001626045093871653, 0.963688850402832 ]
[ 0.001690953504294157, 0.9974919557571411, 0.00046426893095485866, 0.0003528303641360253 ]
en
0.999997
There are no conflicts of interest.
38317305_p22
38317305
Conflicts of interest
0.935876
other
Other
[ 0.02788134478032589, 0.002627171343192458, 0.9694914221763611 ]
[ 0.007451090961694717, 0.9894022941589355, 0.0018082372844219208, 0.0013383196201175451 ]
en
0.999997
Table salt, chemically known by the name sodium chloride (NaCl), is a substance composed of sodium (Na+) and chloride (Cl-) ions , which typically appear as small, white, crystalline granules or as a fine powder, soluble in water, and have a salty taste . Salt is a ubiquitous dietary component consumed worldwide for hu...
PMC467049_p0
PMC467049
Introduction
4.057315
biomedical
Study
[ 0.9922093152999878, 0.0005992113146930933, 0.007191414013504982 ]
[ 0.6204362511634827, 0.0013762667076662183, 0.3779451549053192, 0.00024227968242485076 ]
en
0.999997
Like many other developing countries, iodine deficiency in Tanzania remains a significant public health concern, with adverse effects on cognitive development and growth, particularly among vulnerable populations such as pregnant women and children . The government has implemented various strategies to combat iodine de...
PMC467049_p1
PMC467049
Introduction
4.160915
biomedical
Study
[ 0.9988118410110474, 0.00026432567392475903, 0.0009238136699423194 ]
[ 0.7869142889976501, 0.009771019220352173, 0.20293563604354858, 0.0003790004993788898 ]
en
0.999999
Preferences for salt type are shaped by a complex interplay of cultural, economic, and nutritional factors . While some consumers favor commercially branded salts for their perceived quality, safety, and iodine fortification, others prefer locally sourced salts due to cultural and traditional practices, authenticity, o...
PMC467049_p2
PMC467049
Introduction
3.940753
biomedical
Study
[ 0.9608786702156067, 0.00044621998677030206, 0.03867511823773384 ]
[ 0.8965123891830444, 0.006470379885286093, 0.09686658531427383, 0.00015065216575749218 ]
en
0.999995
The sample preparation method followed the procedure described in Ref. , which involves drying samples, grinding them to a fine powder, weighing them, dissolving them in deionized water, filtering, and dilution to ensure accurate measurement of components, and ensuring the concentration of analytes falls within the cal...
PMC467049_p3
PMC467049
Introduction
4.258196
biomedical
Study
[ 0.9991883635520935, 0.00023941842664498836, 0.0005721948109567165 ]
[ 0.9014507532119751, 0.025559542700648308, 0.07256434857845306, 0.00042539587593637407 ]
en
0.999998
According to Ref. , atomic absorption spectroscopy (AAS) analyzes specific metals in salt samples by measuring the absorbance of light in the gaseous state which is proportional to the amount of the element. The aspirated solution is placed into a flame furnace, converting the metal ions to free atoms and a light beam ...
PMC467049_p4
PMC467049
Introduction
4.149121
biomedical
Study
[ 0.9984447360038757, 0.0002564713067840785, 0.0012987341033294797 ]
[ 0.9993743300437927, 0.00021366165310610086, 0.00037642609095200896, 0.00003553347414708696 ]
en
0.999998
This study was conducted in Nkonkilangi, Mangwanjuki, Unyanga, Chibumagwa, and Kinyambwa villages adjacent to Kitangiri, Singidani, Kindai, Chibumagwa, and Sulunga lakes, respectively located in Singida and Dodoma regions. The lakes are known for their high salinity levels, and salt extraction has been practiced in the...
PMC467049_p5
PMC467049
Study site
1.900902
biomedical
Study
[ 0.8717063665390015, 0.0015461002476513386, 0.12674759328365326 ]
[ 0.9821022748947144, 0.01710137352347374, 0.00040561569039709866, 0.0003906251222360879 ]
en
0.999996
A systematic sampling approach was employed, whereby locally sourced samples of salt were collected from local producers at production sites . Commercially branded samples of salt were collected from retailers at markets and shops in closed packets. To study preferences for salt type, stratified sampling was used, wher...
PMC467049_p6
PMC467049
Study design
2.386184
biomedical
Study
[ 0.9185357689857483, 0.001175308134406805, 0.0802888348698616 ]
[ 0.9971399307250977, 0.002591923577710986, 0.00014383015513885766, 0.0001242889993591234 ]
en
0.999998
The study received ethical approval from the University of Dodoma, under reference number MA.84/261/02. All participants provided written, informed consent. The participants had the option to withdraw from the study at any time if it made them uncomfortable, and the surveys were anonymized.
PMC467049_p7
PMC467049
Ethical clearance
1.362011
biomedical
Other
[ 0.852401077747345, 0.002776731038466096, 0.14482218027114868 ]
[ 0.1549919843673706, 0.8428019285202026, 0.0011198048014193773, 0.0010862632188946009 ]
en
0.999996
A total of 210 samples from seven types of salt (2 commercially branded and 5 local salts) in seven sampling sites-10 samples per site-were picked for one type of salt. In each pack, three samples from the bottom, middle, and top of the package (in total, 30 samples per site) were randomly selected in stores and househ...
PMC467049_p8
PMC467049
Sample collection
2.697188
biomedical
Study
[ 0.8753302693367004, 0.001083936425857246, 0.12358573824167252 ]
[ 0.987168550491333, 0.012420255690813065, 0.0002462895354256034, 0.0001648472825763747 ]
en
0.999997
A local and commercially branded salt sample, distilled water purchased from a local supplier in villages, potassium iodide (KI ) Fisher Scientific USA solution, sodium thiosulphate (Na 2 S 2 O 3 ) Sigma-Aldrich USA solution (titrant), and starch Fisher Scientific USA solution (indicator) of analytical grade purity (>9...
PMC467049_p9
PMC467049
Materials and equipment
2.551131
biomedical
Study
[ 0.9820885062217712, 0.000511719030328095, 0.01739976555109024 ]
[ 0.886954665184021, 0.11185254901647568, 0.0006864828174002469, 0.0005062561831437051 ]
en
0.999996
The iodine concentration in the salt sample was assessed through iodometric titration methods and MBI rapid test kits, India . The PerkinElmer 2380 Atomic Absorption Spectrophotometer USA and, the Lovibond MD 640 Colorimeter Kit 214140 Germany were used to analyze ions in the salt samples . Furthermore, households were...
PMC467049_p10
PMC467049
Procedure
2.587147
biomedical
Study
[ 0.992377519607544, 0.0006358169484883547, 0.006986603606492281 ]
[ 0.9809443950653076, 0.018406860530376434, 0.00033933392842300236, 0.00030940509168431163 ]
en
0.999998
According to Ref. , RTK involves preparing materials, dissolving salt in clean water, applying the solution, and observing the color change on the test strip or reaction pad. The iodine concentration value was then compared to the provided color chart on the RTK packaging. The test was performed in a well-lit area, and...
PMC467049_p11
PMC467049
Rapid test kits (RTK) determination of iodine content
4.110962
biomedical
Study
[ 0.9992615580558777, 0.0002219763700850308, 0.0005165418260730803 ]
[ 0.9959219694137573, 0.0029840623028576374, 0.0010138280922546983, 0.00008022661495488137 ]
en
0.999996
The procedure described in Refs. was followed to determine iodine in salt, where 25 g of potassium iodide (KI) from Fisher Scientific USA was weighed and dissolved in 100 ml of distilled water. Three drops of starch solution were added to create a starch-iodine complex. An excess KI solution was added to the sample to ...
PMC467049_p12
PMC467049
Titration (preparation of sample and standard iodine solution)
4.095
biomedical
Study
[ 0.9993539452552795, 0.00021270544675644487, 0.0004334190452937037 ]
[ 0.9943353533744812, 0.005178064573556185, 0.0003641580115072429, 0.0001224367442773655 ]
en
0.999997
The chemical symbols denote the following compounds: KI stands for potassium iodide solution, Na 2 S 2 O 3 denotes sodium thiosulfate solution, I 2 indicates elemental iodine, NaI represents sodium iodide, and Na 2 S 4 O 6 signifies sodium tetrathionate.
PMC467049_p13
PMC467049
Titration (preparation of sample and standard iodine solution)
2.416751
biomedical
Other
[ 0.9711875319480896, 0.000922837876714766, 0.02788965217769146 ]
[ 0.06889693439006805, 0.9292367100715637, 0.0012663977686315775, 0.0005999670829623938 ]
en
0.85714
Quality control (QC) samples were used to ensure the ongoing accuracy and precision of an analytical method. Samples are analyzed alongside unknown samples and compared to known values. Common QC samples include : Blank samples contain no analyte used to check for contamination. Spiked samples with known quantities of ...
PMC467049_p14
PMC467049
Titration (preparation of sample and standard iodine solution)
4.17149
biomedical
Study
[ 0.9994164705276489, 0.00035070203011855483, 0.000232744961977005 ]
[ 0.9983013868331909, 0.00104007578920573, 0.0005822312668897212, 0.0000762679337640293 ]
en
0.999997
describes the procedure for measuring minerals, which involves collecting salt samples, grinding them into a fine powder, calibrating analytical instruments, and analyzing them using flame photometry and an atomic absorption spectrophotometer. Quality control and safety precautions were adhered to in order to maintain ...
PMC467049_p15
PMC467049
Procedure for the measurement of other minerals
4.141449
biomedical
Study
[ 0.9985824823379517, 0.0003503925399854779, 0.001067155390046537 ]
[ 0.9936466217041016, 0.0042546577751636505, 0.0019640757236629725, 0.0001347170618828386 ]
en
0.999996
Barium Chloride (BaCl₂) Sigma-Aldrich USA solution, which forms a white precipitate of Barium Sulphate (BaSO₄) Sigma-Aldrich, USA, and turbidimetric agents, methylthymol blue, were used to estimate sulphate ions in the salt from the working solution prepared. The presence and concentration of phosphate (PO₄³⁻) were det...
PMC467049_p16
PMC467049
Determination of sulphate, phosphate, and nitrate
4.101046
biomedical
Study
[ 0.9993628859519958, 0.00013993657194077969, 0.0004970924346707761 ]
[ 0.9988864064216614, 0.0008457456715404987, 0.0002224125200882554, 0.00004546360651147552 ]
en
0.999997
Iron (Fe 2 ⁺/Fe³⁺) was determined by the colorimetric method, in which iron ions were detected colorimetrically using 1, 10-phenanthroline, which formed colored complexes with iron and were measured spectrophotometrically. The PerkinElmer 2380 Atomic Absorption Spectrophotometer USA was used to determine manganese in t...
PMC467049_p17
PMC467049
Determination of iron, manganese, and ammonium
4.063122
biomedical
Study
[ 0.9993932247161865, 0.00014014811313245445, 0.0004666254098992795 ]
[ 0.9950149655342102, 0.004505162127315998, 0.0003983760252594948, 0.0000814961749711074 ]
en
0.999995
A survey was conducted in the selected households to gather data on the prevalence of different types of salt (commercially branded vs. local), usage patterns, socio-economic factors influencing choices, reasons for their choice, and frequency of use.
PMC467049_p18
PMC467049
Determination of household salt type prevalence
1.785118
biomedical
Study
[ 0.8167616128921509, 0.0024209413677453995, 0.18081749975681305 ]
[ 0.9633241891860962, 0.03553272783756256, 0.0006302475230768323, 0.0005128618213348091 ]
en
0.999997
The study employed several statistical methods for detailed data analysis [ , , , ]. Descriptive statistics were used to summarize the basic features of the data, including measures of central tendency (mean, median) and dispersion (standard deviation, range) . The Shapiro-Wilk test determined data normality , and comp...
PMC467049_p19
PMC467049
Statistical analysis
4.06028
biomedical
Study
[ 0.9990944862365723, 0.0002516295062378049, 0.0006538141751661897 ]
[ 0.9995524287223816, 0.00012813971261493862, 0.0002789089921861887, 0.00004049597919220105 ]
en
0.999998
Table 2 presents the concentrations of iodine present in commercial-branded and local salt samples collected from different sources. Samples SA, SC, SF, and SG met the regulatory standards set by the WHO for iodine, but SB, SD, and SE didn't comply. There were significant differences in iodine content for salt samples ...
PMC467049_p20
PMC467049
Results and discussion
4.185831
biomedical
Study
[ 0.9990049004554749, 0.00035124144051223993, 0.0006439215503633022 ]
[ 0.9994138479232788, 0.000175856301211752, 0.00036625147913582623, 0.00004406863808981143 ]
en
0.999996
Only one sample of SG showed an iodine level within TBS limits; the rest had a lower iodine content than recommended by TBS . reported that of the 19 common salts tested in the Tanzanian market, only two, 79.95 mg/kg Super Salt and, 76.67 mg/kg Kipepeo met the accepted iodine levels . recommends salt-iodine amount shou...
PMC467049_p21
PMC467049
Results and discussion
3.88705
biomedical
Study
[ 0.998765230178833, 0.00018228065164294094, 0.0010524673853069544 ]
[ 0.9958297610282898, 0.0024589700624346733, 0.0016126642003655434, 0.00009851895447354764 ]
en
0.999996