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5. TruSeq adapter, index 4 |
Source sequence: |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG |
6. TruSeq adapter, index 5 |
Source sequence: |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG |
7. TruSeq adapter, index 6 |
Source sequence: |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTG |
8. TruSeq adapter, index 7 |
Source sequence: |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTG |
9. TruSeq adapter, index 8 |
Source sequence: |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTG |
10. TruSeq adapter, index 9 |
Source sequence: |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG |
11. TruSeq adapter, index 10 |
Source sequence: |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTG |
12. TruSeq adapter, index 11 |
Source sequence: |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTG |
13. TruSeq adapter, index 12 |
Source sequence: |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG |
14. QP1 primer |
Source sequence: |
AATGATACGGCGACCACCGA |
15. QP2 primer |
Source sequence: |
CAAGCAGAAGACGGCATACGA |
Step-by-step library generation |
Step 1. Single-cell lysis |
Input substrate: |
* one isolated cell |
Added oligos/reagents: |
* lysis buffer |
* protease |
Molecular event: |
The single cell is lysed in a small-volume reaction, releasing genomic DNA in the same tube used for downstream library construction. |
Product structure: |
* released genomic DNA from one cell |
Step 2. Lambda spike-in and MspI digestion |
Input substrate: |
* released genomic DNA |
Added oligos/reagents: |
* unmethylated lambda DNA spike-in |
* MspI restriction enzyme |
* Tango buffer |
Subsets and Splits
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