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- o Store at -20°C in 1-ml LoBind tubes. 50% glycerol solution should be equilibrated to room temperature before use. |
## Pipette Calibration |
- l Follow manufacturer's calibration and maintenance schedules. |
- l Pipette accuracy is particularly important when using SPRIselect reagents. |
## Chromium X/iX or X Series Chip Holder |
- l Chromium X/iX or X Series Chip Holders encase GEM-X 3' Chips. |
- l The holder lid flips over to become a stand, holding the chip at 45 degrees for optimal recovery well content removal. |
- l Squeeze the slider on the back (right side) of the holder toward the middle to unlock the lid and return the holder to a flat position. |
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## GEM-X Chip Handling |
- l Minimize exposure of reagents, chips, and gaskets to sources of particles and fibers, laboratory wipes, frequently opened flip-cap tubes, clothing that sheds fibers, and dusty surfaces. |
- l After removing the chip from the sealed bag, use in ≤24 h . |
- l Execute steps without pause or delay, unless indicated. When using multiple chips, load, run, and collect the content from one chip before loading the next. |
- l Fill all unused input wells in rows labeled 1, 2, and 3 on a chip with an appropriate volume of 50% glycerol solution before loading the used wells. DO NOT add glycerol to the wells in the top NO FILL row. |
- l Avoid contacting the bottom surface of the chip with gloved hands and other surfaces. Frictional charging can lead to inadequate priming of the channels, potentially leading to wetting failures. |
- l Minimize the distance that a loaded chip is moved to reach the Chromium X series instrument. |
- l Keep the chip horizontal to prevent wetting the gasket with oil, which depletes the input volume and may adversely affect the quality of the resulting emulsion. |
## GEM-X Chip & Holder Assembly with Gasket |
- l Close the holder lid. Attach the gasket by holding the tongue (curved end, to the right) and hook the gasket on the left-hand tabs of the holder. Gently pull the gasket toward the right and hook it on the two right-hand tabs. |
- l DO NOT touch the smooth side of the gasket. |
- l Open the chip holder. |
- l Align notch on the chip (upper left corner) and the open holder with the gasket attached. |
- l Slide the chip to the left until the chip is inserted under the guide on the holder. |
- l Depress the right hand side of the chip until the spring-loaded clip engages. |
- l Keep the assembled unit with the attached gasket until ready for dispensing reagents. |
One of the rows of the gasket will not align with the GEM-X chip wells. This is expected and will not impact the assay. |
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Tab 6 Text A generic image representative of the GEM-X 3' Chip is shown below. The Chromium X/iX Chip Holder is also referred to as Chromium X Series Chip holder. |
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## GEM -X Chip Loading |
- l Place the assembled chip and holder flat (gasket attached) on the bench with the lid open. |
- l Dispense at the bottom of the wells without introducing bubbles. |
- l When dispensing Gel Beads into the chip, wait for the remainder to drain into the bottom of the pipette tips and dispense again to ensure complete transfer. |
- l Refer to Load GEM-X Chip on page 36 for specific instructions. |
## Chromium X Series Instrument Firmware |
- l Firmware version 2.0.0 or higher is required in the Chromium X/iX instrument to run the GEM-X chips. |
- l Firmware Version 2.2.0 or higher is required in the Chromium Xo instrument to run the GEM-X chips. |
- l The current firmware version of the instrument will be displayed on the instrument homescreen. |
- l Consult the Chromium X Series Instrument User Guide with Readiness Test (CG000396) for detailed instructions on updating the firmware. |
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## Gel Bead Handling |
- l Use one tube of Gel Beads per sample. DO NOT puncture the foil seals of tubes not used at the time. |
- l After removing the Gel Bead strip from the packaging, equilibrate the Gel Bead strip to room temperature for at least 30 min before use. |
- l Store unused Gel Beads at -80°C and avoid more than 12 freeze-thaw cycles. |
DO NOT store Gel Beads at -20°C . |
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