title stringlengths 0 1.13k | abstract stringlengths 1 15.7k | PMID int64 22 36.5M |
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Central nervous system involvement in patients with rotavirus gastroenteritis. | Two children with rotavirus gastroenteritis are presented. The first case developed a fatal Reye's syndrome and the other one encephalitis with slow recovery. The rotavirus diagnosis was made in both cases by electron microscopy and a significant rise in antibody titres to Nebraska calf diarrhea virus was seen in one o... | 204,984 |
Different brain areas mediate the analgesic and epileptic properties of enkephalin. | Single injections of 120 micrograms of methionine-enkephalin were made into various midbrain and forebrain structures in the rat. Analgesia was observed after injections into or near the ventral, caudal midbrain periaqueductal gray matter. Seizures and other pathological electroencephalogram (EEG) changes were seen wit... | 204,998 |
A morphologic and morphometric study of the mitochondria in several hepatoma cell lines and in isolated hepatocytes. | Some characteristics of the mitochondria of hepatocytes and of three hepatoma cell lines have been compared. By means of stereologic analysis of electron micrographs of cross-sections through cells the volume of mitochondria per unit volume of cell cytoplasm and the surface areas of the mitochondrial envelope and crist... | 205,039 |
Immunoelectrophoretic study of Sendae virus envelope antigens with hyperimmune sera prepared in different laboratory animals. | At similar HAI titers rabbit sera reveal a larger number of fractions (ten) in immunoelectrophoretic analysis of Sendai virus envelopes than do guinea pig sera, which only yield three distinct fractions. These differences are observed in both rocket and crossed immunoelectrophoresis, and are more marked in the latter. ... | 205,040 |
Chromosome abberations in rat embryo cells transformed after infection with ultraviolet-irradiated herpes simplex virus. | Two rat embryo cell lines (TR1 and TR2) transformed after infection with ultaviolet-inactivated herpes simplex virus type 2 showed modal chromosome numbers in the tetraploid range. In the TR1 cell line a near-diploid cell population replaced during subcultivation the hypotetraploid cells thus explaining the loss of imm... | 205,041 |
[Spontaneous tumors in rats from the Rappolovo Breeding Nursery of the Academy of Medical Sciences of the USSR]. | The authors report the data on the incidence of neoplasms in rats of the "Rappolovo" nursery breeding (USSR Acad. Med. Sci.) and their offsprings during 4 sequential generations kept in the vivarium of the N. N. Petrov Research Institute of Oncology of the USSR Ministry of Health. The frequency of spontaneous tumors in... | 205,047 |
The presence of complement fixing antibodies to the avian group-specific ("gs") sarcoma-leukosis antigen in the human population and in cancer patients. | Antibodies to the avian group-specific "gs" sarcoma-leukosis antigen were made evident by complement fixation reaction in human sera collected from apparently healthy subjects and from patients with different forms of cancer and non-neoplastic diseases. The number of sera investigated was of 2,123. The possible mechani... | 205,042 |
No demonstrable accumulation of 14C-vitamin D3 in developing rat teeth and bones. | The distribution of 14C-vitamin D3 administered subcutaneously in arachidic oil to 6-day-old suckling rats has been examined autoradiographically. The animals were sacrificed 1, 2, 4 and 8 days after injection. The autoradiographic exposure time was 4 months. The sectioning and exposure was performed in a freeze-room. ... | 205,084 |
Sonography of the common bile duct: value of the right anterior oblique view. | The common hepatic and proximal common bile ducts (common duct) lie anteriorly and generally to the right of the portal vein in the porta hepatis. This constant anatomic relationship can be used to demonstrate the common duct and to differentiate it from the portal vein by gray scale ultrasonography. The patient is sca... | 205,124 |
Immunoglobulin inclusions in non-Hodgkin's lymphomas. | The occurrence and characteristics of intracellular immunoglobulin inclusions in non-Hodgkin's lymphomas are described. Lymphoma cells from three patients with immunoblastic sarcoma contained classic cytoplasmic, periodic acid-Schiff (PAS)-positive Russell bodies. Immunoperoxidase staining revealed monoclonal IgG (k) i... | 205,130 |
Burro aortic collagen: platelet aggregating activity and ultrastructural changes induced by plasma. | A fibrillar collagen molecule was extracted from the upper thoracic aorta of an old burro (Equus asinus). Presence of the collagen in the extract was determined by amino acid analysis, scanning and transmission electron microscopy, incubation with collagenase, and assays of its platelet-aggregating capacity by "aggrego... | 205,146 |
Comparison between adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs. | Adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs was studied. Both aluminum hydroxide and activated sludge flocs adsorbed greater amounts of poliovirus than rotavirus. Aluminum hydroxide flocs reduced the titer of poliovirus in tap water by 3 log10, but they only reduced the titer... | 205,173 |
Thalamic lesions during the development period and their clinical correlation. | 28 autopsy cases were investigated in which the thalamus was impaired in early life, i.e. prenatal, perinatal, and postnatal (under 1 year of age). The thalamus is vulnerable to brain damage in the perinatal and early postnatal period. Lesions in the thalamus are severe in the DM, PL and VPL, and moderate in the VL and... | 205,171 |
The peripheral lymphocyte count as an aid in the clinical staging of lung cancer. | The association between disease staging and peripheral lymphocyte count (PLC) was studied in 178 patients with histologically verified lung cancer. Mean PLC varied inversely with the stage of disease (p less than .01). The mean values were 2821, 2217, and 1594 for Stages I, II and III respectively. Thoretical normal di... | 205,178 |
Hyporesponsiveness of lymphocytes to virus antigens in rheumatoid arthritis. | The immune response of peripheral blood lymphocytes to measles, rubella, parainfluenza types 1, 2, and 3 RNA virus antigens and to varicella-zoster and herpes virus type 1 DNA virus antigens was evaluated in 14 patients with rheumatoid arthritis (RA) and 14 matched controls by assessing 3H-thymidine incorporation. The ... | 205,223 |
Distribution of enzyme of nucleic acid metabolism in cow snout epidermis. | Certain enzymes participating in the degradation of DNA have been measured in cow snout epidermis, liver, kidney and intestinal epithelium. High activities were found in epidermis compared to other tissues. The enzyme profile of the epidermis was investigated in relation to depth. Those enzymes directly concerned with ... | 205,236 |
The orientation of a heme of cytochrome c oxidase in submitochondrial particles. | The electron paramagnetic resonance of the low spin signal from oxidized cytochrome c oxidase has been studied in oreinted multilayers of submitochondrial and electron transport particles. Measurements of the angular variation of the EPR spectra with the multilayer plane orientation allow the determination of the heme ... | 205,240 |
Optimum interaction of sterol side chains with phosphatidylcholine. | The specificity of the interaction between the cholesterol side chain and egg phosphatidylcholine was precisely defined by examining the effect of three new analogues of cholesterol with modified side chains on the ordering of two steroid spin labels in liposomes. The complete side chain of cholesterol was shown to be ... | 205,247 |
Specific release of plasma membrane enzymes by a phosphatidylinositol-specific phospholipase C. | The release of plasma membrane ecto-enzymes by a phosphatidylinositol-specific phospholipase C from Staphylococcus aureus was investigated. There was no effect on L-leucyl-beta-naphthylamidase, alkaline phosphodeisterase I and Ca2+- or MG2+-ATPase, but substantial proportions of the alkaline phosphatase and 5-nucleotid... | 205,248 |
The effect of corticotropin on phospholipid metabolism in isolated adrenocortical cells. | Trypsin-dispersed cat adrenocortical cells were incubated at 37 degrees C in modified Eagle's medium containing [14C]arachidonic acid of sodium [14C]-acetate and then in non-radioactive medium. Radioactive incorporation was obtained in all phospholipids, with the greatest amount of radioactivity in phosphatidylcholine,... | 205,250 |
A comparative spectroscopic study of two non-haem iron proteins lacking labile sulphide from Desulphovibrio gigas. | The ultraviolet visible, and near infrared spectrum of a two-iron protein from Desulphovibrio gigas, a new type of non-haem iron protein lacking labile sulphide, is compared with that of D. gigas rubredoxin. The charge transfer band maxima of rubredoxin at 495 and 565 nm are less separated in the new protein implying a... | 205,263 |
An alternative to group homes. | This paper describes a supported lodgings scheme as an alternative to group homes. It is pointed out that the County Council has a statutory duty to finance supported lodgings and that schizophrenics are ideally suited to such a scheme. Some short-stay, the majority of the 'new' non-demented long-stay, and a large numb... | 205,302 |
Clinically silent gross hypergastrinaemia from a multiple hormone-secreting pancreatic apudoma. | A patient is described who had a malignant pancreatic islet cell apudoma secreting corticotrophin (ACTH) and melanocyte-stimulating hormone (MSH), both of which were clinically active, and very large quantities of immunoreactive gastrins, which were biologically active but clinically silent (normal gastric acid secreti... | 205,304 |
Alteration of sodium transport in mouse mammary epithelium associated with neoplastic transformation. | Using electrophysiological techniques we have examined the apical membrane ionic permeabilities of primary cell cultures of the mouse mammary gland in the midpregnant, preneoplastic, and neoplastic states. Membrane Na+ permeability changed with tumorigenesis, whereas K+ and Cl- permeabilities were unaltered. With trace... | 205,364 |
Characterization of a unique cell line (LAZ 221) from human acute lymphocytic ("null" cell) leukemia. | A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the "null" cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12,+(9q12q). Both the established cell line and the pati... | 205,365 |
Radiology of needle biopsy of pulmonary lesions. | A technique for percutaneous aspiration needle biopsy is described and discussed. It is concluded that it forms a simple and easy way of obtaining histological material and it can be carried out in most radiological departments. | 205,383 |
Effect of antitonin on blood pressure in the one-kidney hypertensive rat. | 1. The tonin concentration of saliva and submaxillary glands was studied in one-clip hypertensive rats with or without the contralateral kidney. 2. Salivary tonin concentration was elevated in one-kidney hypertensive rats, but not in one-kidney normotensive or two-kidney, one-clip hypertensive rats. In contrast, an ele... | 205,386 |
The fetal hypothalamus and pituitary in the initiation of labour. | Evidence is presented for a number of events in the fetal hypothalamic-pituitary axis which may play a key role in the onset of labour: (1) In the sheep fetus a progressive rise in the fetal circulating concentrations of corticotropin in the days preceding delivery; (2) In the human fetus a switch from the production o... | 205,396 |
[Photosensitivity in children with malign tumors (author's transl)]. | Photosensitivity (photoconvulsive reaction = PCR) has to be regarded as a genetically determined EEG-symptom, which is seen with markedly age dependent in up to 15% of healthy individuals. Correlations with epileptic and nonepileptic diseases (migraine, anorexia nervosa, psychovegetativ instability) are known. 157 chil... | 205,403 |
The influence of exogenous and of membrane-bound phosphatidate concentration on the activity of CTP: phosphatidate cytidylyltransferase and phosphatidate phosphohydrolase. | Rat liver microsomes were treated with phospholipase D to obtain microsomal membranes with varying amounts of membrane-bound phosphatidate. This treatment did not impair the activity of two microsomal-bound enzymes acting with phosphatidate as substrate, i.e. CTP: phosphatidate cytidylyltransferase and phosphatidate ph... | 205,412 |
Exchange of tritium from randomly tritiated taurocholate by microbial bile salt oxidoreductases. | Tritium distribution on randomly labelled taurocholate (TC) was estimated at 28%, 4%, 1% and less than 0.5% on the hydrogens opposite the 3alpha-, 7alpha- and 12alpha-OH groups and taurine moiety respectively. Anomalously, C. perfringens 3alpha-hydroxysteroid dehydrogenase (3alpha-HSDH) catalyzed tritium loss of 36% on... | 205,428 |
Inhibition of prostaglandin-induced cyclic AMP accumulation in the rat anterior pituitary by alrestatin. | Alrestatin at 25-1 X 10(-4) M inhibited the accumulation of cyclic AMP induced by prostaglandin E2, but not theophylline, in the rat anterior pituitary in vitro. Somatostatin, at lower concentrations, inhibited both; maximal inhibition of the prostaglandin effect was greater with alrestatin. As cyclic AMP is considered... | 205,429 |
Neuronal responses to extracellularly applied cyclic AMP:Role of the adenosine receptor. | At low doses, theophylline blocks the neuronal depressant effects of 5'-AMP, but not cyclic AMP. Higher doses (100 mg/kg) block cyclic AMP responses and reduce the effects of noradrenaline and GABA. It is concluded that cyclic AMP does not depress neurones via the adenosine receptor. | 205,430 |
Effect of two phosphodiesterase inhibitors, cyclic adenosine 3':5'-monophosphate, and a beta-blocking agent on human sperm motility. | Mann-fructose fluid (MF), and MF plus caffeine, MF plus pentoxifylline, MF (dibutyryl cAMP), MF plus propranolol, and MF plus propranolol plus dibutyryl cAMP were individually added to aliquots of semen samples obtained from 18 normal men. These drugs were added to a final concentration of 0.6 mM. One aliquot with no a... | 205,445 |
[Operon study of riboflavin biosynthesis in Bacillus subtilis. XII. The determination of the ATP:riboflavin-5'-phosphotransferase and riboflavinsynthetase content in the cells with varying genotypes]. | Activities of riboflavinkinase and riboflavinsynthetase were measured in 15 strains of Bacillus subtilis with different genotype. The increased level of riboflavinkinase was observed in strains, resistant to lumiflavin or lumichrome. Specific activity of riboflavinkinase was found to be about 100 times lower than that ... | 205,483 |
[Genetic control over 7 Lpm-system allotypes of very high-density serum alpha2-lipoprotein the mink]. | Population and hybridological analyses have demonstrated that the alloantigenic markers of very high density alpha2-lipoprotein of mink serum, Lpm7 and Lpm8, along with Lpm1, Lpm2, Lpm3, Lpm4, Lpm5, belong to a common immunogenetic system. 21 Lpm-phenotypes are determined at least by 36 genotypes; each of the 11 phenot... | 205,484 |
Persistent and boosterable IgE antibody production in mice injected with low doses of ovalbumin and silica. | The results of the present experiments show that Aerosil, an amorphous submicroscopic silica, is able to stimulate in Swiss outbred mice the production of IgE antibodies to a single 1 microgram dose of Ovalbumin (OA) in experimental conditions in which detectable IgG1 antibodies were not produced. The production of IgE... | 205,512 |
Involvement of the sympathetic nervous system in the urinary bladder internal sphincter and in penile erection in the anesthetized cat. | Male cats were anesthetized with pentobarbital. In one series of experiments a Foley catheter was placed suprapubically in the urinary bladder and physiologic saline, under a constant head of pressure, was allowed to flow at a constant rate through the bladder. Alpha-adrenergic stimulants such as ephedrine, norepinephr... | 205,522 |
Catabolism of very low density lipoprotein B apoprotein in man. | The turnover and the catabolic fate of the B apoprotein of very low density lipoprotein (VLDL-B) was studied in 15 normal and hyperlipidemic subjects using reinjected autologous VLDL labeled with radioiodine. The specific radioactivity-time curve of the B apoprotein in total VLDL (S(f)20-400) was multiexponential but c... | 205,554 |
Multiple effects of N, N' dicyclohexyl carbodiimide on the beta-adrenergic receptor--adenylate cyclase system in frog erythrocytes. | Treatment of frog erythrocytes with N,N' dicyclohexylcarbodiimide (DCCD) leads to a loss of catecholamine stimulated adenylate cyclase activity without any decrease in fluoride or PGE1 stimulated cyclase. However, the concentrations of the reagent which inhibit catecholamine sensitive adenylate cyclase activity are 10 ... | 205,559 |
Effect of adrenocorticotropic hormone on plasma glucocorticoids and antidiuretic hormone of cattle exposed to 20 and 33 C. | Four open, dry Holstein cows in the University of Missouri Climatic Laboratory were used to study the effect of adrenocorticotropic hormone administration on glucocorticoids and antidiuretic hormone of plasma in two reversal trials at thermoneutral (20 C, 50% relative humidity) and heat (33 C, 50% relative humidity) co... | 205,564 |
Purification and partial characterization of the apoA-I of rabbit high density lipoprotein. | Purification of apoA-I from rabbit high density lipoproteins (HDL) gave one single band in sodium dodecylsulphate-polyacrylamide disc electrophoresis and reacted only with antiserum to apoA-I. The molecular weight was about 25000. The amino acid composition of rabbit ,poA-I gave a difference index of 7.4 compared to hu... | 205,614 |
Trauma to the skin causes recurrence of herpes simplex in the mouse. | Mild trauma was induced in the skin of mice latently infected with herpes simplex virus type I by stripping the originally infected ear with cellophane tape. Recurrent herpes simplex developed at this site 2 to 5 days later. It was detected clinically by the development of erythema and vesicles and by the appearance of... | 205,629 |
Cyclic excretion of hepatitis A virus in experimentally infected chimpanzees: biophysical characterization of the associated HAV particles. | Experimental infection of two chimpanzees with the Phoenix Antigen strain of HAV resulted in the cyclic excretion of virus particles on days 9-11, 14-15, and 20-21 postinoculation. Isopycnic banding in CsCl of stool suspensions prepared from 9-11; 14-15; and 17, 19, 21 dav stool pools revealed multiple buoyant densitie... | 205,631 |
Antiviral action of a rifamycin derivative: formation Rous sarcoma virus particles deficient in 60 to 70S RNA. | Growth of Rous sarcoma virus-transformed cells in the presence of rifazone-8(2), a rifamycin derivative, results in the formation of noninfectious virus particles lacking 60 to 70S RNA. | 205,684 |
The clinical, radiologic, and pathologic characterization of benign hepatic neoplasms. Alleged association with oral contraceptives. | Poor histopathologic documentation and confusing terminology have caused focal nodular hyperplasia (FNH) and liver cell adenoma (LCA) to be categorized together as "benign hepatomas." FNH and LCA are distinguishable grossly, microscopically, and ultrastructurally. In a majority of instances they may be differentiated b... | 205,759 |
Methyl methanesulphonate mutagenesis in L5178Y mouse lymphoma cells. | The alkylating agent MMS was toxic to mouse lymphoma L5178Y cells and decreased their growth rate. A dose-dependent induction of thioguanine- and thymidine- but not ouabain-resistant variants was observed. The prolonged period for expression of thioguanine-resistant variants observed with other mutagens was also found ... | 205,781 |
Vitamin-D-dependent rickets type II. Resistance of target organs to 1,25-dihydroxyvitamin D. | Studies were done to determine the cause for hypocalcemia, secondary hyperparathyroidism, osteomalacia and osteitis fibrosa cystica in a 22-year-old black woman. The patient had normal serum 25-hydroxyvitamin D (14 ng per milliliter) and markedly elevated serum 1,25-dihydroxyvitamin D (137 pg per milliliter). Vitamin D... | 205,789 |
Polyneuropathy from inhalation of N2O cartridges through a whipped-cream dispenser. | A generalized toxic polyneuropathy was identified in a 23-year-old woman after excessive intentional inhalation of compressed N2O delivery from cartridges through a whipped-cream dispenser. The chronology of the patient's N2O abuse correlated clearly with two episodes of recurrent polyneuropathy. The toxic effects were... | 205,815 |
Nutrient intake and serum cholesterol level in normal children 6 to 16 years of age. | A study of 103 healthy, white schoolchildren who were between 6 and 16 years of age revealed no positive correlation between the level of serum cholesterol and the mean daily amount of total calories, cholesterol, fat, saturated fat, or sugar in the diet. | 205,827 |
A case of short rib polydactyly. | A patient with neonatal dwarfism had many characteristics of the Majewski syndrome, except for a cleft lip and abnormalities of internal organs. Absence of these anomalies and presence of more severe pathological changes in bone suggest either a wider spectrum for this disorder or a separate entity. | 205,829 |
Sleep and memory: reproduction of syntactic structures previously evoked within REM sleep-related reports. | 7 subjects, aged 19 to 25 yr., who evoked syntactic verbal material in the report made after awakening during REM sleep, were asked to reproduce it a few minutes later. No big differences had been obsered between the two recalls; all recalled verbal materials, many sentences and kernel-sentences reappeared. These resul... | 205,832 |
Oncogenicity of the C-type virus HL-23V in marmosets and characterization of virus isolated from an HL-23V-induced marmoset tumor: comparison with simian sarcoma virus type 1. | Dog thymus cells chronically infected with HL-23V, a C-type virus isolated from human acute myelogenous leukemia cells, produced both transforming and nontransforming virus indistinguishable from simian sarcoma virus type 1 (SSV-1/SSAV-1) and induced fibromas in newborn marmosets. All inoculated marmosets developed ant... | 205,850 |
Transfer factor and its protective effect against herpesvirus infections of marmosets. | Using skin test response and lymphocyte blastogenesis as indicators of cell-mediated immunity, we have been able to demonstrate in vivo transfer of cell-mediated immunity to marmosets both with dialyzable transfer factor (TFd) prepared from a human donor and transfer factor (TF) from baboon whole cell lysates. We were ... | 205,856 |
Species-specific cellular DNA-binding proteins expressed in mouse cells transformed by chemical carcinogens. | Mouse cells transformed by DNA and RNA tumor viruses and by chemical carcinogens have been examined for the presence of specific DNA-binding proteins by DNA-cellulose chromatography. Using mouse DNA-cellulose we have obtained single-stranded DNA-binding proteins from two clones transformed by chemical carcinogens. Simi... | 205,864 |
Insulin and epidermal growth factor-urogastrone: affinity crosslinking to specific binding sites in rat liver membranes. | Both insulin and human epidermal growth factor-urogastrone (EGF/URO) can be covalently linked to specific rat liver membrane binding sites by glutaraldehyde coupling followed by sodium borohydride reduction to yield affinity-labeled membrane constituents sufficiently stable for solubilization and further analysis by va... | 205,865 |
Ergot alkaloids and cyclic nucleotides in the CNS. | Ergotamine and dihydroergotamine show a higher blocking effect towards the dopamine-induced stimulation of adenylate cyclase activity than to the apomorphine-induced stimulation. This could reflect a possible specificity of the compound to dopaminergic receptor. Bromocriptine blocks in a more competitive way the activa... | 205,889 |
Origin of intranuclear inclusions in myeloma cells. | The mechanism of inclusion of the cytoplasmic dense bodies into the nucleus of myeloma cells was studied by transmission electron microscopy. The findings in different cells suggest that the inclusion of the electron-dense bodies is a result of an interaction between the nuclear envelope and the endoplasmic reticulum. ... | 205,940 |
Induction of stalk and spore cell differentiation by cyclic AMP in slugs of Dictyostelium discoideum. | Multicellular masses of the cellular slime mold Dictyostelium discoideum, under conditions which ordinarily suppress cell differentiation, develop clusters of stalk cells and spore cells when implanted with Sephadex particles that had been soaked in 5 X 10(-3)M cyclic adenosine monophosphate (AMP). A possible relation ... | 205,945 |
The genome of simian virus 40. | The nucleotide sequence of SV40 DNA was determined, and the sequence was correlated with known genes of the virus and with the structure of viral messenger RNA's. There is a limited overlap of the coding regions for structural proteins and a complex pattern of leader sequences at the 5' end of late messenger RNA. The s... | 205,947 |
Collagenase immunolocalization in cultures of rheumatoid synovial cells. | Cultures of rheumatoid synovial cells that have been enzymatically dissociated and are adherent to a culture vessel are morphologically heterogeneous. When these cells are cultured on a collagenous substrate for 2 to 6 days at 37 degrees C in serum-free medium, they produce collagenase. A monospecific antibody to human... | 205,952 |
[Polypous nonchromaffin paraganglioma of the stomach]. | It is reported on the unusual position of a nonchromaffin paraganglioma in the stomach, which in gastroscopy imposed as polyp and was removed. On the basis of the light-microscopic picture, its behaviour during staining and the electronmicroscopic investigations the tumour is classified in the group of the paragangliom... | 206,030 |
[Repeated characterization, analysis of variance limitation and discrimination analysis classification of EEG-activity patterns in human sleep]. | 1. To describe quantitatively and to deliminate nine EEG sleep patterns, mean values and standard deviations of abundances of the frequencies 0.8 ... 1.8 c/sec, 2...3.5 c/sec, 4...13c/sec, 14 to 17 c/sec, 18 to 22 c/sec, and 23 to 40 c/sec as well as of the average amplitudes in selected frequency ranges were calaculat... | 206,077 |
Ethylmorphine-N-demethylation by liver homogenate of newborn and adult rats; Enzyme kinetics and age course of Vmax and Km1. | Optimum incubation conditions for determination of ethylmorphine-N-demethylation with newborn and adult rat liver homogenate have been determined: 1 ml 1:20 liver homogenate in 1.15% KCl, 1 ml 0.1 M phosphate buffer with ethylmorphine, NADP, and glucose-6-P, final concentrations 10, 0.33 and 5 mM, respectively, no nico... | 206,078 |
Lack of correlation between adrenal cAMP and corticosteroidogenesis in the newborn rat. | In 2 to 16-day-old rats subjected to heat, the adrenal content of cAMP did not increase in animals younger than 14 days of age, but serum corticosterone was significantly higher than control values at all ages except in 10 and 12-day-old animals. This would suggest that cAMP may not mediate the adrenal steroidogenic re... | 206,080 |
Mixed mesenchymal and neuroectodermal tumor of the cerebellum. | An unusual cerebellar tumor composed of mixed mesenchymal and neuroectodermal elements in a 44 year old man is reported. The mesenchymal element is well-differentiated adipose tissue, whereas the neuroectodermal components exhibit a variety of medulloblastomatous, astrocytomatous, oligodendrogliomatous and ependymomato... | 206,094 |
Marked elevation of serum angiotension-converting enzyme and hepatic fibrosis containing long-spacing collagen fibrils in type 2 acute neuronopathic Gaucher's disease. | Serum angiotensin-converting enzyme in a patient with type 2 acute neuronopathic Gaucher's disease (242 nmol/min/ml) was 10.8 times higher than values for eight patients with other hereditary neurologic abnormalities (22.5 +/- 2.0) and 9.4 times higher than those for 12 patients with other diseases (25.7 +/- 2.6) (P le... | 206,129 |
Abortive and productive infections of human mononuclear phagocytes by type I herpes simplex virus. | The ability of Type I herpes simplex (HSV) to replicate in normal human monouclear phagocytes was investigated. Mononuclear leukocytes were obtained from the peripheral blood of patients by Ficoll-Hypaque gradient centrifugation, and the monocytes were isolated by allowing the cells to adhere to tissue culture dishes. ... | 206,146 |
Isolation of transmissible gastroenteritis virus from pharyngeal swabs obtained from sows at slaughter. | A virologic survey was conducted to determine the frequency of transmissible gastroenteritis (TGE) virus infection in farm-raised sows. Pharyngeal swab specimens collected in an abattoir were examined for TGE virus by inoculation onto swine-testes cell culures. The virus was detected in 61 (3%) of a sample of 2,058 Iow... | 206,173 |
Comparison of the lesions of Aleutian disease in mink and hypergammaglobulinemia in ferrets. | Gross and microscopic lesions of Aleutian disease (AD) in mink and hypergammaglobulinemia in ferrets were compared. Both conditions were characterized by widespread proliferation of plasma cells, but proliferation was more prominent in mink infected with AD. Arteritis did not occur in hypergammaglobulinemic ferrets. Mi... | 206,172 |
[Glucocorticoid receptors in mammary tumors in PS and C3H mouse strains (author's transl)]. | Specific binding of glucocorticoids was characterized in mammary tumors and cells derived from C3H and PS mice. The binding protein satisfied all the general criteria of hormone receptors namely high affinity and binding specificity for glucocorticoids (KD congruent to 2.5 nM) for 3H Dexamethasone and limited number of... | 206,179 |
[Effect of estradiol-17 beta on the somatrotroph cells of the adenohypophysis in the newt (author's transl)]. | The somatotroph cells of the female newt's adenohypophysis are characterized by PAS positive, acidophils, electron dense large inclusions (4-6 micrometer). The apparition of these inclusions has been studied in females during the puberty. A correlation between the ovary stages and apparition, number and ultrastructural... | 206,180 |
[A case of virilizing adrenal cortical adenoma whose steroid profile evokes a partial 21-hydroxylase deficiency (author's transl)]. | The C19 and C21 urinary steroids from a virilizing adrenal tumour with high levels of plasma 17 alpha-hydroxyprogesterone and its urinary metabolites have been identified and quantitated gas chromatography and mass spectrometry of sephadex fractions of the total urinary extract. A of the fifty-five identified steroids ... | 206,181 |
Influence of cyclic 3',5'-adenosine monophosphate and adenosine on vascular reactivity. | cAMP, 5'-AMP and adenosine in doses of 1, 2 and 5 mg/kg i.v. in rats diminish vascular resistance in the hind limbs in vivo and in isolated limbs perfused with nutrient fluid containing adrenaline (A) (10(-3) M), slowing action of the heart and lowering blood pressure. After administration of cAMP, 5'-AMP and adenosine... | 206,222 |
Growth comparisons of avian infectious bronchitis virus strains in organ cultures of chicken tissues. | Six strains of avian infectious bronchitis virus (IBV) were used to inoculate explants of a range of 15 chicken tissues and virus growth kinetics observed over a period of 96 hours thereafter. Similar patterns of virus production were given by all 6 strains from explants of any particular tissue such as the nasal turbi... | 206,229 |
Experimental infection of chickens with hemorrhagic enteritis virus. | Chickens were experimentally infected with hemorrhagic enteritis virus, causing lesions similar to those observed in turkeys. Lesions were induced by intraperitoneal and oral infection. Virus particles with the morphology of adenovirus were demonstrated in the spleens of infected chickens. Splenic extracts from infecte... | 206,247 |
Enhancing and inhibiting effects of spleen cells from tumour-bearing mice on growth of virus-induced primary sarcoma. | The effects of adoptive transfer of spleen cells from tumour-bearing mice on the growth of Moloney murine sarcoma virus (M-MuSV)-induced primary tumours in BALB/c mice were studied. The effects were in 2 directions, depending on the time of lymphocyte inoculation; when spleen cells from tumuor-bearing mice 12 days afte... | 206,273 |
[Persistency of serum antibodies against Entamoeba histolytica]. | With the purpose to determine the duration of serum antibodies against E. histolytica by means of the contraimmunoelectrophoresis technique, 31 patients with the diagnosis of intestinal or liver invasive amebiasis were included in this study. In 92.3% of the 13 patients subjected to transversal study, no specific antib... | 206,270 |
[Cytochrome concentration in the liver at different stages of adaptation to cold]. | Concentration of cytochroms in the liver of adult male rats has been studied during the acclimation to cold. One group of rats was kept at about 24 degrees, another at 2--4 degrees. After a month of acclimation to cold the cytochroms concentration in the liver increased, whereas the mitochondrial concentration of cytoc... | 206,288 |
[Study of the responses of tumor and normal tissues to exposure to ionizing radiation by the EPR method. I. Comparison of the low temperature EPR spectra of irradiated tumor and normal tissues]. | ESR spectra of liver samples of normal mice and liver tumour samples of mice (line C3HA) injected hepatomo 22-a irradiated in the dose of 1 Mrad at --196 degrees C were studied. The ESR spectra of normal and tumour tissues irradiated at --196 degrees C are shown to differ. Only the ESR spectra of tumour and liver of th... | 206,290 |
Some comparative properties and localization of porcine jejunal adenylate cyclase. | Cholera toxin is thought to cause intestinal secretion by activating adenylate cyclase and increasing intracellular 3',5'-cyclic AMP concentrations in intestinal mucosa. Cholera toxin causes profuse secretion of fluid into ligated intestinal loops of both pigs and rabbits, but cholera toxin-induced increases in 3',5'-c... | 206,324 |
Alkyl methane sulfonate mutation of diploid human lymphoblasts and Salmonella typhimurium. | Concentration dependence of mutation in equigenerational exposures to methyl, ethyl, propyl, and butyl methanesulfonates has been determined in diploid human lymphoblasts and Salmonella typhimurium. Forward mutation was measured at the hypoxanthine guanine phosphoribosyltransferase locus in human lymphoblasts and at th... | 206,353 |
Radioimmunoassay for protein p28 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera and breast cancer extracts. | The main protein of the core of murine mammary tumor virus, with a molecular weight of 28,000 (p28), was solubilized by deoxycholate treatment of the virus and purified by Ultrogel ACA-54 filtration and hydroxyapatite chromatography. This protein was used as labeled antigen in a highly specific and reproducible radioim... | 206,358 |
[Susceptibility to avian tumor viruses: determination of chick phenotypes]. | The genetic cellular susceptibility to avian sarcoma viruses (RSV) of subgroups A, B, C and E has been determined in one week old Chicks. Fibroblasts from pin feathers were grown in vitro and tested by focus formation after infection. This technique will allow, for the first time, the study of the influence of the host... | 206,373 |
[Receptors for vasoactic intestinal peptide (VIP) in human colonic adenocarcinoma membranes: specific binding and stimulation of adenylate cyclase]. | Human colonic adenocarcinoma plasma membranes exhibit specific receptors for VIP. Adenylate cyclase activity is stimulated by a VIP concentration as low as 10(-10) mol/1. | 206,381 |
Metabolic effects of some antibilharzial drugs: inhibition of NAD-linked substrate oxidation in rat liver by bilharcid. | BILHARCID inhibited malate and pyruvate oxidation. The degree of inhibition reached 50% when the concentration of Bilharcid was 10(-3) M. The mechanisms of the inhibitory action of the drug are discussed with reference to the effects of both adenine and pyridine nucleotides on the activity of the oxidoreductase enzymes... | 206,427 |
Synthesis of an oligonucleotide inhibitor of protein synthesis in rabbit reticulocyte lysates analogous to that formed in extracts from interferon-treated cells. | A heat-stable, low-molecular-weight inhibitor of protein synthesis is formed on incubation of haemin-supplemented rabbit reticulocyte lysates with ATP and double-stranded RNA (dsRNA). It inhibits the translation of both added encephalomyocarditis virus RNA (EMC RNA) and endogeneous messenger RNA in reticulocyte lysates... | 206,430 |
Electron transport in the cni-1 mutant of Neurospora crassa. | 1. Mitochondria from the nuclear mutant cni-1 have no optically detectable cytochrome aa3 in early log phase growth. These mitochondria have a high level of respiration that is not inhibited by cyanide but is inhibited by salicylhydroxamic acid. They also show a substantial amount of cyanide-sensitive respiration. 2. A... | 206,437 |
Effect of prolactin on tetracycline binding to human spermatozoa. | The effect of different concentrations of prolactin (0 to 60 ng) on the release of tetracycline from human spermatozoa labeled with 7-3H-tetracycline hydrochloride was studied. Prolactin significantly enhanced the release of bound tetracycline, indicating that prolactin may influence the processes associated with sperm... | 206,470 |
A chromosomal translocation causing overproduction of iso-2-cytochrome c in yeast. | The CYC7-1 mutation in the yeast Saccharomyces cerevisiae causes the production of approximately 30 times the normal amount of iso-2-cytochrome c. Genetic analysis established that the CYC7-1 mutation is a reciprocal translocation involving the left arm of chromosome V and the right arm of chromosome XVI. The chromosom... | 206,486 |
Effect of somatostatin in the Syrian hamster bearing a transplantable islet-cell tumor. | The influence of somatostatin (SRIF) on blood glucose, plasma insulin and plasma glucagon was studied in hamsters bearing a transplantable islet-cell tumor secreting insulin and glucagon as well as in normal controls. Fed anesthetized animals were infused intraperitoneally either at a dose of 10 microgram in 15 min or ... | 206,496 |
Inhibition of Ca2+-activated cyclic nucleotide phosphodiesterase reaction by a heat-stable inhibitor protein from bovine brain. | An inhibitor protein of cyclic nucleotide phosphodiesterase is demonstrated in bovine brain extract and separated from modulator binding protein, a recently discovered inhibitory factor of phosphodiesterase. The new inhibitor protein is similar to the cyclic AMP phosphodiesterase inhibitor from bovine retina (Dumler, I... | 206,547 |
Fine structural localization of potassium-stimulated rho nitrophenylphosphatase activity in denrites of the cerebral cortex. | A histochemical technique for the demonstration of K+-rho-nitrophenylphosphatase (K+-rhoNPPase) activity, a component of the Na+,K+-ATPase, has been applied at the fine structural level in the somatosensory cortex of the rat. Reaction product was consistenly found in dendrites and in association with the cytoplasmic as... | 206,562 |
Infections with viruses and Mycoplasma pneumoniae during exacerbations of chronic bronchitis. | The association of viral and Mycoplasma pneumoniae infections with acute exacerbations of chronic bronchitis was studied by serologic or isolation techniques in 46 adult men during the five years from 1964 through 1968. Serologic evidence of viral or M. pneumoniae infection was detected in 25% of 166 episodes of exacer... | 206,630 |
Human foamy virus: further characterization, seroepidemiology, and relationship to chimpanzee foamy viruses. | A foamy virus present in human nasopharyngeal carcinoma tissue was studied for a number of biological properties, including range of cellular susceptibility, growth curve, evolution of cytopathic effect in relation to cellular fusion and intracellular viral distribution, reverse transcriptase activity, and buoyant dens... | 206,631 |
The effect of helper virus on Abelson virus-induced transformation of lymphoid cells. | Abelson murine leukemia virus (A-MuLV)-transformed fibroblast nonproducer cells were used to prepare A-MuLV stocks containing a number of different helper viruses. The oncogenicity of the A-MuLV stocks was tested by animal inoculation and their ability to transform normal mouse bone marrow cells was measured in vitro. ... | 206,646 |
Regulation of interferon production by dibutyryl cyclic GMP in serum-free human diploid cell cultures. | The mechanism for regulating interferon production was investigated in relation to accentuation of production in serum-free human diploid cells (strain WI-38) treated with N2,O2-dibutyryl guanosine 3',5'-cyclic monophosphate (db-cyclic GMP). Interferon production in serum-free WI-38 cell cultures in response to Newcast... | 206,656 |
A host range difference between herpes simplex virus types 1 and 2. | The infectivities of several strains of herpes simplex virus type 1 and type 2 were measured by plating on established human and Chinese hamster cell lines. Whereas all the type 1 strains reproducibly showed a 10(4)-fold lower titre on the Chinese hamster cells, the type 2 strains showed only a 10- to 100-fold lower ti... | 206,659 |
Bovine and ovine leukemia viruses. I. Characterization of viral antigens. | A comparative study on several virus-specific antigens of bovine and ovine leukemia viruses is presented. In addition to the major immunologically reactive, nonglycosylated antigen p21, which has a molecular weight of 21,000, both viruses share two glycoproteins of apparent molecular weights of 60,000 (gp60) and 32,000... | 206,723 |
In vitro RNA transcription by the New Jersey serotype of vesicular stomatitis virus. I. Characterization of the mRNA species. | The in vitro RNA synthesis by the virion-associated RNA polymerase of vesicular stomatitis virus (VSV), New Jersey serotype, was compared with that of the serologically distinct Indiana serotype of VSV. The New Jersey serotype of VSV synthesized five distinct mRNA species in vitro, three of which were smaller than the ... | 206,724 |
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