title stringlengths 0 1.13k | abstract stringlengths 1 15.7k | PMID int64 22 36.5M |
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Experimental study of a potential anti-asthmatic agent: SCH 15280. | A forced oscillations technique for measuring total respiratory system resistance was used to quantitate the bronchomotor activity of Sch 15280: (5[4-(N-methyl)-piperidylidine]5H-[1]-benzopyrano[2,3,b]-pyridine maleate) and to compare its potency to that of standard bronchodilator agents. By the intravenous route, Sch ... | 237,114 |
Etonitazene. An improved synthesis. | 1-(BETA-Kiethylaminoethyl)-2-(p-ethoxybenzyl)-5-nitrobenzimidazole (u, etonitazene) is a potent analgesic that has value in drug addiction studies. We have developed a simple high-yield synthesis of 1 that is adaptable to alrge scale preparations. The synthesis involves the condensation of 2-(beta-diethylaminoethylamin... | 237,125 |
Kinetic study of neutrophil and inflammatory fluid beta glucuronidase. | Beta glucuronidase obtained from rat inflammatory fluid and granulocytes was studied kinetically. Linear increases in activity occurred with time and enzyme concentration. No evidence of enzyme degradation during incubation was obtained. Supernatant and granulocyte enzyme activity was identical in relation to Km, and p... | 237,313 |
Genetic control of the in vitro responses of rat blood lymphocytes. I. Comparison of in vitro and in vivo responses. | Nielsen, H. E. & Koch, C. Genetic Control of the In Vitro Responses of Rat Blood Lymphocytes. I. Comparison of In Vitro and In Vivo Responses, Scand. J. Immunol. 4, 31-36, 1975. In vitro DNA synthetic responses of blood lymphocytes from the inbred rat strains BN, Lewis, and AS were compared with each other after st... | 237,318 |
[Cerebral sclerosis. Diagnostic criteria and differential diagnostic consideration in practice]. | In"cerebral arteriosclerosis" the diffuse sclerotic involvement of the cerebral vessels may produce acute softening of cerebral tissue. However this paper concentrates mainly on the clinical symptomatology which, in the absence of major vascular accidents, is characterized from the psychopathologic viewpoint by acute c... | 237,320 |
Total body potassium, acid-base status and serum electrolytes in acute diarrhoeal disease. | Total body potassium (TBK), acid-base status and serum electrolyte concentrations were measured in 49 children suffering from acute diarrhoeal disease, on admission and after rehydration. The TBK was usually low and the lowest values were found in those with the lowest pH. There was no relationship between TBK and seru... | 237,325 |
Anxiety: its nature and treatment. | Anxiety is an unpleasant, diffuse emotion, directed towards the future and associated with feelings of threat to the individual. Clinical anxiety occurs when a patient suffers from anxiety which is more frequent, more severe or more persistent than he is used to and can tolerate. Almost a third of the adult population ... | 237,328 |
Retransplantation of human bone marrow. Two immunosuppressive drug regimens. | A 15-year-old boy with aplastic anemia was successfully retransplanted with matched sibling bone marrow after failure of a first transplant from the same donor. Cyclophosphamide was used as immunosuppression for the first transplant, and cyclophosphamide plus procarbazine plus antithymocyte globulin were used for the s... | 237,352 |
In line final filters for removing particles from amphotericin B infusions. | The feasibility of using membrane filters to remove particles from intravenous infusions of amphotericin B in dextrose 5% (a colloidal solution) was studied. Six types of commercial membrane filters, ranging in pore size from 0.45-1.0 mum, were used. Because of the effect of pH on the particle size of colloidal solutio... | 237,416 |
A primate model for testing anticonvulsant drugs. | Senegalese baboons (Papio papio), with a natural syndrome of photosensitive epilepsy, consistently show generalized myoclonic jerks if stimulated stroboscopically at hourly intervals, two to eight hours after the intravenous administration of allylglycine, 200 mg/kg. This provides a model for testing the acute antiepil... | 237,498 |
[Gamma-glutamyltransferase activity in the human red blood cell membrane (author's transl)]. | A gamma-glutamyltransferase activity is found in the human red blood cell membrane. Membrane isolation was carried out according to the method of Dodge et al. (Dodge, J. T., Mitchell, C. and Hanahan, J. (1963) Arch. Biochem. Biophys. 100, 119-130) (modified) and proteins were solubilized either with 1% sodium deoxychol... | 237,550 |
Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain. | The major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields ... | 237,552 |
Studies on the kinetics of glycosidases from chemically-induced rat colonic tumours and normal rat colon. | K-m values of beta-N-acetylglucosaminidase (2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase EC 3.2.1.30), beta-N-acetylgalactosaminidase (EC 3.2.1.53), beta-galactosidase (beta-D-galactoside galactohydrolase EC 3.2.1.23) and alpha-L-fucosidase (alpha-L-fucoside fucohydrolase EC 3.2.1.51) of distal col... | 237,555 |
[Purification and characterization of two peroxidases from hard wheat]. | Two peroxidases A and B were purified from a borate buffer extract (pH = 10,4) of durum wheat semolina (Triticum durum), var. Bidi 17, by chromatography on DEAE-cellulose, salting out by 3M ammonium sulphate and two chromatographies on CM-cellulose; specific activities of peroxidase A or B were increased 114 or 66 fold... | 237,578 |
Dissociation of theophylline uptake and inotropic effect in myocardial tissue: influence of temperature, pH and calcium. | 1. The myocardial uptake and the positive inotropic effect of theophylline (100 mug/ml; 0.56 mM) were studied in isolated electrically driven guinea-pig hearts perfused by the Langendorff technique under various extracellular conditions. [3H]-theophylline was used. 2. Variations in temperature, hydrogen ion and calcium... | 237,594 |
Effect of pentazocine on the evoked potentials recorded in the primary somesthetic cortical areas of guinea pigs. | The influence of pentazocine on the evoked potentials registered in the primary somesthetic cortical area in guinea pigs under pentobarbital anesthesia was studied. The stimulation was applied in the contralateral lip and the evoked cortical responses from different zones on the cortex stereotaxically localized were re... | 237,601 |
Doses of epinephrine causing arrhythmia during enflurane, methoxyflurane and halothane anaesthesia in dogs. | The arrhythmogenic dosage of epinephrine, administered by constant intravenous infusion, was measured in five dogs during enflurane, methoxyflurane and halothane anaesthesia. While premature ventricular contractions were observed in only one of five dogs with enflurane and methoxyflurane, epinephrine-induced arrhythmia... | 237,613 |
Carboxylesterases (EC 3.1.1). A comparison of some kinetic properties of horse, sheep, chicken, pig, and ox liver carboxylesterases. | A comparative study of the kinetic be,avior of horse, sheep, chicken, pig, and ox liver carboxylesterases is reported. The enzymes exhibit similar specificites towards a series of phenyl esters in which the acyl group is varied, and towards a series of butyrate esters in which the alcohol group is varied. Non-Michaelis... | 237,618 |
Kinetics and reaction mechanism of the carbamylphosphate synthetase of a multienzyme aggregate from yeast. | We have studied the kinetics and reaction mechanism of the carbamylphosphate synthetase of an enzyme aggregate functioning in the pyrimidine pathway of yeast. MG--ATP was found to be one of the substrates of the enzyme reaction which was activated by free Mg-2+ and inhibited by free ATP. Feedback inhibition by UTP was ... | 237,624 |
Generation of hypophysiotropic activity in hypothalamic extracts in vitro. | The luteinizing hormone (LH) and follicle stimulating hormone (FSH) releasing activity, as well as the prolactin (PRL) release-inhibiting activity were measured in both neutral aqueous, and acid ethanolic extracts of rat hypothalami. LH and FSH-releasing activities were detectable only in the latter type of extract, wh... | 237,752 |
Glutamine-dependent asparagine synthetase in fetal, adult and neoplastic rat tissues. | Three enzyme reactions related to asparagine synthesis were studied in rat tissues: formation of aspartylhydroxamate, either from aspartate or by transfer from asparagine, and actual synthesis of asparagine from aspartate. Actual asparagine synthesis occurred at one-thousandth the rate of the other two reactions. Opti... | 237,754 |
The conformation of the RNA in cowpea chlorotic mottle virus: dye-binding studies. | The binding of the dye acridine orange to cowpea chlorotic mottle virus (CCMV) and its purified RNA has been studied to obtain the number of dye-binding sites as a function of pH and, through further analysis, to estimate the degree of RNA secondary structure in situ. Acridine organe does not bind to CCMV protein and s... | 237,760 |
Nature of the Kanagawa phenomenon of Vibrio parahaemolyticus. | In a study of the Kanagawa phenomenon of Vibrio parahaemolyticus, both Kanagawa-positive and -negative strains were found to produce hemolytic factors that could not be differentiated on Wagatsuma blood agar. The presence of fermentable carbohydrates in media containing high concentrations of NaCl promoted the growth o... | 237,836 |
A study of the unfolding of the inhibited subtilisin in guanidine hydrochloride. | Inhibited subtilisin (Subtilism Carlsberg; Subtilopeptidase A) is unfolded in the presence of 7 M guanidine hydrochloride. The unfolding reaches a maximum in approximately 6 min at 20C at pH 8.0. This is demonstrated by an increase of the mean residue ellipticity at 222 nm from -8.02 x 10-3 to -1.72 x 10-3 deg. cm-2/de... | 237,844 |
Flavin nucleotides, glutathione reductase and assessment of riboflavin status. | The in vitro effects of flavin nucleotides on human erythrocyte glutathione reductase were studied. Flavin mononucleotide was found to be a potent inhibitor of glutathione reductase in haemolysates, having an effect at a concentration of 1 muM. Flavin adenine dinucleotide inhibited at a concentration at least ten fold ... | 237,845 |
The stability constants of magnesium oxalate complexes. | The stability constants of MgC204 and Mg2C204-2nCOmplexes were estimated at 37--38 C by four different techniques; there was good agreement among the results of the different techniques. The stability constant of MgC204 is (4019 plus or minus 76 M-minus 1). The stability constant of Mg2C204-2nWAS FOund to be less than ... | 237,850 |
Enzyme histochemical observations on the segmentation of the proximal tubules in the kidney of the female rat. | The segmentation of the proximal tubules in the kidney of the female rat was studied by means of enzyme histochemical reactions and the results compared with those observed in male and recently described by Jacobsen and J0rgensen (1973 a). Reactions were performed for the following soluble, coezyme-dependent oxido-redu... | 237,855 |
Threonine deaminase from Escherichia coli. II. Maturation and physical properties of the enzyme from a mutant altered in its regulation of gene expression. | The biosynthetic L-threonine deaminase (L-threonine hydrolase deaminating, EC 4.2.1.16) has been purified from Escherichia coli K12 regulatory mutant CU18. This mutant has properties that follow the predictions of the autogregulatory model previously proposed for the control of synthesis of the isoleucine-valine biosyn... | 237,884 |
Effect of N-bromosuccinimide modification on dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli. Activity, spectrophotometric, fluorescence and circular dichroism studies. | When dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli B, MB 1428, is treated with approximately a 5 mol ratio of N-bromosuccinimide (NBS) to enzyme at pH 7.2 and assayed at the same pH, there is a 40% loss of activity due to the modification of 1 histidine residue and possibly 1 methioni... | 237,891 |
Reaction heat variation with pH in formation of the trypsin-soybean inhibitor complex. | The heat of reaction between beta-trypsin and Kunitz soybean inhibitor (STI) hasbeen measured at 5 degrees and 25 degrees from pH 4 to 8.5. Corresponding measuremenportion of tRNA-Gly2-GGA/G molecules isolated from E. coli cells. The missense suppressor mutation, glyTsuA36(HA), results in a C yields U base substituti... | 237,923 |
Effect of ploidy on spontaneous mutation rate to asparagine non-requirement in cultured cells. | Variations in ploidy do not affect the spontaneous mutation rate to asparagine non-requirement in Jensen rat sarcoma cells cultivated in vitro. | 237,929 |
The precipitation of proteins by carboxymethyl cellulose. | Carboxymethyl cellulose (CMC) formed insoluble complexes with beta-lactoglobulin, bovine serum albumin and Na caseinate. Maximum precipitation of the beta-lactoglobulin-CMC complex occurred at pH 3-2, whereas maximum precipitation of the bovine serum albumin. The percentage of protein precipitated by CMC decreased with... | 237,942 |
The influence of cosolvents and substrate substituents on the sorption of benzoic acid derivatives by polyamides. | The sorption of some substituted benzoic acid derivatives by polyamides (nylons) from aqueous solution has been examined and the influence of their nature together with those of the cosolvent and polymer have been assessed. In all cases the sorption isotherms were linear and could be expressed by a simple distribution ... | 237,995 |
Non-ionic surfactants and membrane transport of thioridazine in goldfish. | Polysorbate 80, which has been widely used in studies of the effects of surfactants on drug absorption, increases the rate of absorption of some drugs at concentrations near its critical micelle concentration (cmc). To determine whether all non-ionic surfactants were capable of inducing this effect, the effects of six ... | 237,996 |
Dextromethorphan inhibits 5-hydroxytryptamine uptake by human blood platelets and decreases 5-hydroxyindoleacetic acid content in rat brain. | The effect of dextromethorphan on the uptake and metabolism of 5-hydroxytryptamine (5-HT) was studied in human blood platelets and in rat brain. In the concentration of 120 nM dextromethorphan inhibited the uptake of 5-HT (1 mu-M) into platelets by 50%. The corresponding concentrations of imipramine and methadone under... | 238,000 |
Effect of beta receptor blockade on the initiation and perpetuation of ouabain-induced ventricular tachyarrhythmia. | The role of the cardiac beta receptor in the genesis and perpetuation of ouabain-induced ventricular tachyarrhythmias (VT) was investigated in the chloralose (20 mg/kg)-urethane (200 mg/kg) anesthetized dog. This was achieved by determining: 1) the effect of oxprenolol (0.3 mg/kg), a potent beta receptor blocking agent... | 238,019 |
Rate-dependent Effects of drugs. II. effects of some major tranquilizers on multiple fixed-ratio, fixed-interval schedule performance. | The effects of promazine, chlorpromazine, triflupromazine, prochlorperazine, trifluoperazine, perphenazine, fluphenazine, haloperidol, benzquinamide, tetrabenazine and chlorprothixene were determined on the rate of conditioned key pecking of pigeons under a multiple fixed-ratio 30, fixed-interval 5-minute schedule of f... | 238,028 |
A comparative evaluation of the treatment of typhoid fevers with co-trimoxazole and chloramphenicol in Egypt. | Seventy-two patients with bacteriologically proven enteric fever were treated with either co-trimoxazole (41 cases) or chloramphenicol (31 cases). C0-trimoxazole was found to be superior to chloramphenicol in relieving the toxaemia. The average number of days required for patients to become afebrile was 5-5 days in the... | 238,046 |
Mechanism for the paradoxical aciduria following alkali administration to prolonged-fasted patients. | Rapidly induced systemic alkalinization due to either sodium-lactate or sodium-bicarbonate infusion in prolonged-fasted subjects with steady-state ketoacidosis was associated with a decrease in urine pH. This decrease in urine pH from 5.50 to 5.20 was the result of a significant decrease in urinary ammonium excretion f... | 238,093 |
[Examination of the acute effect of prednisolone on the acid secretion of the stimulated gastric mucosa in man]. | In comparison with the maximal acid stimulation produced by intravenous infusion of histalog (2 mg/kg bw/h), a single intravenous injection of 25 mg prednisolone during maximal acid stimulation produced by pentagastrin (1.5 mug/kg bw/h) does not cause a significant change of secretion rate or ionic composition of gastr... | 238,129 |
Smallpox vaccination reactions, prophylaxis, and therapy of complications. | Smallpox vaccination in the United States is a routine public health measure which has been under intensive review during the last decade. The most frequently occurring adverse reactions to vaccination are benign and require little or no systemic therapy. These reactions include accidental infection, erythematous and u... | 238,178 |
Necleoside conformations. 19. Temperature and pH effects on the conformation of guanosine phosphates. | Proton magnetic resonance spectra at 250 MHz were measured as a function of temperature and pH of the three guanosine phosphates. From these data and previously published work the conformational parameters of these compounds were determinated. The phosphate group of Guo-5'-P changes its conformation around the C-O bond... | 238,183 |
Enzymatic S-de-ethylation and the mechanism of S-dealkylation. | An NADPH- and oxygen-dependent enzymatic system responsible for the formation of acetaldehyde from ethylthioethers is present in the 9000 g supernatant fraction of rat liver. This system appears to be quite similar to the one responsible for the formation of formaldehyde from methylthioethers. Although both of these sy... | 238,213 |
Separation of mouse spleen haematopoietic cell fractions for transplantation purposes in lethally irradiated mice. | Mouse cells were fractioned by adherence chromatography on glass beads. The fractionation of spleen cells yielded 5 different fractions, whose transplantation effect was tested by the method of Till and McCulloch. The results of these transplantation tests showed that the chosen form of adherence chromatography was not... | 238,253 |
The carbamate equilibrium of bovine hemoglobin at 37 degrees C. | emoglobin-bound CO2 was estimated by a procedure first described by Rossi-Bernardi et al. (1969) in which the carbamate compound is stabilized by rapid mixing with alkali and then separated from other CO2 constituents in solution by gel filtration and ion-exchange chromatography. Carbamate equilibrium of bovine hemoglo... | 238,266 |
Comparison of benorylate and ibuprofen in the treatment of established rheumatoid arthritis. | Benorylate and ibuprofen appear to be useful drugs for pain relief in rheumatoid arthritis but benorylate would appear to have a slightly better effect on the pain score and it improved the grip strength more than the moderate doses of ibuprofen. It could be a most useful drug when gastric problems limit the dose of as... | 238,275 |
Anthopleurine: a sea anemone alarm pheromone. | The sea anemone Anthopleura elegantissima responds with characteristic contraction to a pheromone released by wounded conspecifics. The alarm pheromone was isolated by ion-exchange chromatography and identified by chemical and spectroscopic methods as the quaternary ammonium ion (3-carboxy-2, 3-dihydroxy-N, N, N-trimet... | 238,286 |
The relationship between length of incubation, bacterial growth and tuberculin yield of a strain of Mycobacterium tuberculosis. | The relationship was studied between length of incubation in a liquid synthetic medium, amount of bacterial growth, pH of the culture filtrate and tuberculin yield of a single strain, DT, of Mycobacterium tuberculosis. No sinple relationship was found between the total crop of bacteria and the tuberculoprotein content ... | 238,317 |
[Annual dynamics of acidobasic indices in the sheep blood]. | Altogether 218 sheep were followed for a year and the values of their acidobasic indices were examined, the follow-up comprises all seasons of the year, gravidity period, time of nursing and milk production. The actual pH value in the sheep blood varied from 7.30 to 7.48, with 7.40 average. The average value of partial... | 238,330 |
[Values of acido-basic balance in the blood of the beagle breed dog in comparison with the German shepherd dog]. | The values of acidobasic balance were examined in 101 dogs of the Beagle breed and in 20 German Sheep-Dogs. The Beagle dogs were divided into five age groups. A difference was found between the values obtained in the group of the youngest dogs and those in older age groups. In comparison with Beagle dogs, the values we... | 238,331 |
Experimental porphyria induced by 3-(2,4,6-trimethylphenyl)-thioethyl)-4 methylsydnone. | Administration of 3-[2-(2,4,6-trimethylphenyl)-thioethyl]-4-methylsydnone (TTMS) induces hepatic porphyria in rats, mice and dogs. The protoporphyrin pigment in livers of rats and mice is found mainly in bile ducts and leads to bile duct proliferation and portal inflammation. Dog livers contain protoporphyrin predomina... | 238,335 |
Ribonuclease activity of Entamoeba histolytica. | Ribonuclease activity of Entamoeba histolytica was purified 65-fold by calcium phosphate gel, ammonium sulphate and acetone treatments. The enzyme was inhibited by metal ions, like Mg-2+, Mn-2+, Co-2+ and Ca-2+. Metal chelating and sulphydryl agents showed no effect. EDTA and alpha, a'-dipyridyl reversed the inhibition... | 238,352 |
Microsomal ethanol oxidation: activity in vitro and in vivo. | Studies by several investigators have confirmed that the microsomal fraction of mammalian liver oxidizes ethanol to acetaldehyde in a reaction that requires NADPH and oxygen. Efforts to identify the enzymes involved have produced conflicting opinions of the reaction mechanism, however. Initially, the microsomal mixed f... | 238,369 |
Transport of H plus and other electrolytes across isolated gastric mucosa of the rabbit. | The relationship of transmural and cellular phenomena to the rate of spontaneous luminal HH+ secretion by short-circuited gastric mucosa bathed in HCO3- and CO2-free Ringer solution was studied by the pH-stat technique. The bulk of luminal acidification can be accounted for by the appearance of H+ and not by organic ac... | 238,401 |
Anion effects on cation movements during correction of potassium depletion. | The differential effects of KCl vs. KHCO3 for intravenous K+ repletion were evaluated in a series of nephrectomized dogs previously depleted of 20% of total-body K by a 2-wk regimen consisting of K-free diet plus DOCA plus chlorothiazide. Twenty-four percent of K losses (5% of original TBK) were replaced by a 2-h infus... | 238,404 |
In vivo erythrocyte sickling in the Japanese sika deer (Cervus nippon): methodology. | The Japanese sika deep (Cervus nippon) proved to be a suitable animal model for the study of acute phases of in vivo erythrocyte sickling. Ophthalmologic studies can be conducted during or after 1 to 6 hours of effective in vivo sickling. Intravenous administration of 1.75 to 3.5% sodium bicarbonate solution at a rate ... | 238,437 |
Inactivation of enterovirus by glutaraldehyde. | A study on the rate of inactivation by glutaraldehyde of coxsackievirus was conducted using different concentrations, temperatures, and pH values. It was found, that 2 percent glutaraldehyde at pH 7.4 and 25 C, as recommended for a sporicide, reduced the titer of infectious virus by 2 log10U in 1 min or less. The reduc... | 238,467 |
Common mesophilic anaerobes, including Clostridium botulinum and Clostridium tetani, in 21 soil specimens. | A relatively rich medium was markedly superior to a dilute medium for the isolation of anaerobic bacteria from soil. The obligate anaerobes isolated from 21 soil samples were all clostridia and the counts ranged from 2.7 X 10-2 to 3.3 X 10-6 per g. The organisms most frequently isolated were Clostridium subterminate, C... | 238,468 |
Necrotizing vasculitis. Etiologic aspects of immunology and coagulopathy. | Thirty-one patients with cutaneous necrotizing vascultis were studied for immunological and coagulation disturbances. Serum immunoglobulin levels did not correlate with tissue deposition of the corresponding immunoglobulins in the lesions of cutaneous necrotizing vasculitis. In all instances, localization of immunoglob... | 238,473 |
Timolol maleate, a new beta-adrenergic receptor blocking agent. | Some pharmacodynamic properties of an oxypropanolamine substituted novel heterocyclic compound are described. Initial studies involve comparison of the racemic mixture, dl-timolol maleate, with the beta-adrenergic receptor blocking agent propranolol in the rat, dog and cat. dl-Timolol maleate is shown to be a potent in... | 238,479 |
Predictive value of various parameters for the antihypertensive effect of the beta blocker ICI 66,082 (1). | The antihypertensive effect of the new beta-blocker, ICI 66,082 was studied in 37 patients with renal or essential hypertension. A daily dose of 75 mg produced a significant antihypertensive effect and a further blood pressure decrease was obtained by increasing the daily dose up to 300 mg; at this treatment level, the... | 238,480 |
Genetic expression of beta-mannosidase activity in different tissues of mice. | Beta-Mannosidase activity of liver, kidney, and spleen of two inbred strains of mice and their crosses has been assayed with the synthetic aubstrate p-nitrophenyl-beta-d-mannoside. Activity is low in C57BL/Kl mice and high in DBA/2/Kl mice. Hybrid animals have intermediate levels of beta-mannosidase activity. Segregati... | 238,502 |
[Changes in imipramine induced noradrenaline potentiation by varying activity of gastric juice under oral medication]. | From the serial studies on imipramine dependent noradrenaline (NA) rise at different values of gastric acid with 336 registrations of blood pressure, it follows that a lawful relation exists between imipramine absorption and gastric juice acidity. At pH values of 3.5--6.0 a distinct decreased absorption of orally suppl... | 238,541 |
Ventilation and blood-gas studies during experimentally produced Mendelson's syndrome in the dog. | Hydrochloric acid was instilled into the bronchial tree in anaesthetized dogs. A severe respiratory acidosis resulted. The metabolic component of acid-base balance was little affected. | 238,546 |
Hypertension in the immediate postoperative period. | Sixty patients out of 1,844 recovery room admissions had significant postoperative arterial hypertension. Nearly 60% of them had a history of hypertension. The postoperative hypertension usually began within 30 min from the end of operation and lasted about 2 hours. In 20% of the patients it lasted 3 hours or longer. C... | 238,547 |
The binding of azide to human methemoglobin A0. Error analysis for the interpolative and noninterpolative methods. | The binding azide to human methemoglobin A0 has been studied at 6 degrees, pH 7, and I = 0.2 by three spectroscopic methods: (1) the conventional interpolative method, (2) an interpolative dialysis technique, and (3) a noninterpolative method. The interpolative methods assume that the fractional spectral change equals ... | 238,572 |
Hydrogen exchange kinetics changes upon formation of the soybean trypsin inhibitor-trypsin complex. | The hydrogen exchange kinetics of the complex of trypsin-soybean trypsin inhibitor (Kunitz) have been compared to the calculated sum of the exchange kinetics for the inhibitor and trypsin measured separately. The exchange rates observed for the complex are substantially less than the sum of the exchange rates in the tw... | 238,590 |
Surface-bound aspartate aminotransferase on collagen films. Compared properties with native enzyme. | Aspartate aminotransferase (L-aspartate : 2-oxoglutarate aminotransferase, EC 2.6.1.1) has been covalently bound to chemically activated collagen films. This enzyme had never previously been coupled to any other solid support. The coupling method, including acyl azide formation on the carrier, allowed coupling of many ... | 238,597 |
Resolution, purification and characterization of the orthophosphate releasing activities from fracture callus calcifying cartilage. | Callus calcifying cartilage alkaline phosphatase was resolved by DEAE-cellulose column chromatography into two distinct phsophatase activities. The phosphatase activity which was eluted first from the column, (phosphatase I), was active towards a variety of phosphate esters, sodium pyrophosphatase and several linear po... | 238,599 |
Fluorescence polarization studies on the conformational transition of bovine plasma albumin in acidic solutions. | The acid-induced isomerization (the N-F transition) and expansion of bovine plasma albumin were studied by measuring fluorescence polarization and lifetime of the excited state of tryptophyl fluorophors. Most of the changes (decreases) in the reciprocal of fluorescence polarization and lifetime of the excited state cor... | 238,615 |
Effect of hydrogen ion concentration on energy metabolism in pig platelets. | Respiration and glycolysis of pig platelets suspended in a dialyzed plasma were studied at various hydrogen ion concentrations. Respiration of platelets was high at acidic pH and decreased at physiological pH. This pH profile may not be attributed to properties of mitochondria, since the respiratory rate of mitochondri... | 238,623 |
Some characteristics of the prostaglandin synthesizing system in rabbit kidney microsomes. | The prostaglandin synthesizing system in rabbit kidney microsomes was characterised using a radiometric assay. Three prostaglandins (F2alpha, E2 and D2) were formed form (1-14C)arachidonic acid, a small amount of prostaglandin A2 was also detected but this was formed non-enzymatically. Biosynthesis was stimulated by re... | 238,646 |
Primary immunodeficiency and malignancy. | Evidence indicating an essential relationship between immunologic deviation and cancer has accumulated rapidly over the past 15 years. Following a brief review of the enormous body of literature linking experimental immunodeficiency and cancer, this discussion will center on humans with primary immunodeficiency disorde... | 238,676 |
Increased susceptibility to infection in sickle cell disease: defects of opsonization and of splenic function. | Patients with sickle cell disease (SCD) have an increased susceptibility to bacterial infections and die more frequently from infection than any other cause. Their sera do not promote the phagocytosis of pneumococci normally, apparently because of an inability to utilize the alternate pathway for C3 fixation as a means... | 238,677 |
Combined alpha- and beta-adrenoceptor blocking drug AH 5158: further studies on alpha-adrenoceptor blockade in anaesthetized animals. | 1. AH 5158, 5-(1-hydroxy-2-((1-methyl-3-phenylpropyl)amino)ethyl)salicylamide, competitively antagonised phenylephrine-induced vasopressor responses in anaesthetized dogs, thus confirming that the drug possesses alpha-adrenoceptor blocking activity. 2. In contrast, AH 5158 was a relatively ineffective antagonist of vas... | 238,705 |
Natriuresis following fourth ventricle perfusion with high-sodium artificial CSF. | Perfusion of the fourth cerebral ventricle with high-sodium artificial cerebrospinal fluid was found to result in an increase in urinary sodium excretion in anesthetized cats. The natriuresis was accompanied by an increase in blood pressure and glomerular filtration rate. However, in animals with the changes in blood p... | 238,725 |
Observations on trace proteins in plasma of febrile patients by cationic disc electrophoresis in acrylamide gel at pH 3.8. | Cationic disc electrophoresis at pH 3.5 in 6 M urea-containing acrylamide gels permits analysis of plasma and other body fluids for the presence of trace proteins with pI greater than or equal 5 and M.W. less than 60,000. These charge and size characteristics would include rabbit and human granulocytic pyrogen, human m... | 238,735 |
Control of myocardial oxygen tension by sympathetic coronary vasoconstriction in the dog. | The effect of sympathetic alpha-receptor coronary vasoconstriction on myocardial oxygen tension was studied in open- and closed-chest, chloralose-anesthetized dogs. Blood oxygen tension in the coronary sinus and blood flow in the circumflex coronary artery were continuously measured in a three-part experiment. With sti... | 238,753 |
Effects of neurotransmitters injected into the posterior and the anterior septal artery on the automaticity of the atrioventricular junctional area of the dog heart. | The effects of neurotransmitters injected into the posterior and the anterior septal artery on the automaticity of the atrioventricular (AV) junctional area were examined in the excised, blood-perfused canine AV node preparation. An AV rhythm of 57.4 +/- 2.8 beats/min (N = 28) developed after the destruction of the sin... | 238,754 |
Calcification of the heart and great vessels. | Calcification of the heart and great vessels is secondary to those diseases which cause a significant alteration of tissue matrix. Metabolic derangements can cause microscopic calcification of heart muscle, but this has not been demonstrated conclusively roentgenographically. The deposition of calcium may follow a sing... | 238,789 |
Double-blind trials of clemastine ('Tavegil') in allergic rhinitis. | Two double-blind randomised trials are reported comparing the effectiveness of the antihistamines, clemastine and chlorpheniramine in comparable doses, in relieving the symptoms of allergic rhinitis. In the first trial, treating 58 adults seen in a general practice, both drugs were prescribed in tablet form; l mg. clem... | 238,790 |
The active transport of carbohydrates by Escherichia coli. | The active transport of carbohydrates by Escherichia coli is discussed with particular reference to (1) identification of an uptake process as 'active transport', (2) nature and control of transport proteins, and (3) mechanisms of energy transduction. (1) The use of substrate analogues, of mutants blocked in metabolism... | 238,808 |
The mitochondrial ATPase. Evidence for a single essential tyrosine residue. | 1. Evidence is presented which indicates that inactivation of the mitochondrial ATPase from bovine heart by the reagent 4-chloro-7-nitrobenzofurazan results from modification of one tyrosine residue per enzyme molecule. Activity can be restored by a variety of sulphydryl reagents. 2. In sodium dodecyl sulphate, the nit... | 238,839 |
Biotin carboxyl carrier protein in barley chloroplast membranes. | Biotin localized in barley chloroplast lamellae is covalently bound to a single protein with an approximate molecular weight of 21 000. It contains one mole of biotin per mole of protein and functions as a carboxyl carrier in the acetyl-CoA carboxylase reaction. The protein was obtained by solubilization of the lamella... | 238,845 |
Rates of onset and offset of action of narcotic analgesics in isolated preparations. | In isolated preparations of the myenteric plexus--longitudinal muscle of the guinea-pig ileum and the mouse vas deferens, the rates of onset and offset of action of narcotic analgesics are inversely related to lipid solubility; this effect is probably due to drug binding at secondary lipid-rich binding sites. These fin... | 238,862 |
Reduction of adverse acute cardiovascular actions of 6-hydroxydopamine in dogs by adrenergic receptor blockade. | Injection of dogs with 50 mg/kg of 6-hydroxydopamine (6-OHDA) caused large increases in blood pressure and heart rate, followed after 1 or 2 hr by severe hypotension. Prior administration of the adrenergic receptor blocking agents phentolamine and propranolol attenuated both the hyper- and hypotensive phases of 6-OHDA.... | 238,861 |
Effect of graft-versus-host reaction on the immune response to alloantigens and growth of a syngeneic tumor. | The graft-versus-host reaction (GVHR) was evoked in (C57BL/6J times C3H/6J times DBA/2J) F1 hybrids by grafting parental lymphoid cells. Groups of bothe F1 hybrids were immunized with thymocytes carrying Thy-1.1 (theta AKR) antigen and still other groups of (C57BL/6J times C3H/HeJ) F1 hybrids were immunized with thymoc... | 238,866 |
Studies on the binding of amylopectin sulfate with gastric mucin. | Amylopectin sulfate, a sulfated polysaccharide that has an antipeptic property, was examined for its ability to bind gastric mucins. After chemically cross-linking the amylopectin sulfate into an insoluble gel, its binding with mucins isolated from antral and fundic mucosa of canine stomachs was studied with chromatogr... | 238,895 |
[The clinical importance of abnormal heart rate tracings during labour (author's transl)]. | A continuous fetal heart rate tracing of the first and second stage of labour was available for 221 labours and deliveries with varying histories of the present pregnancy. The importance of the abnormalities of the fetal heart rate in these tracings was interpreted in comparison to the development of fetal acidosis. Th... | 238,901 |
Effect of gastric secretory inhibitors on the gastric mucosal barrier. | The effects of the synthetic prostaglandin 16, 16 dimethyl-E2 methyl ester (16-diMe-PGE2) and the histamine H2-receptor antagonist metiamide on the gastric mucosal barrier have been studied using the Heidenhain pouch dog model. The 16-diMe-PGE2 caused significant change in the ionic permeability of the mucosa when inst... | 238,904 |
Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. V. Isocitrate: NADP+ oxidoreductase (decarboxylating) and malate: NADP+ oxidoreductase (decarboxylating). | Improved histochemical techniques for the demonstration of NADP+-specific isocitrate dehydrogenase and malate dehydrogenase in tissue sections are described. With these techniques a semipermeable membrane is interposed between the incubating solutions and the tissue sections preventing diffusion of enzymes into the med... | 238,922 |
Inherited susceptibility of cattle to high-altitude pulmonary hypertension. | This study examines the hypothesis that susceptibility of cattle to high-altitude pulmonary hypertension and heart failure (high mountain disease) is genetically transmitted. Eight offspring of cattle recovered from high mountain disease were considered "susceptible." Eleven offspring of healthy cattle residing at high... | 238,929 |
Transport of L-4-azaleucine in Escherichia coli. | The uptake of L-4-azaleucine was examined in Escherichia coli K-12 strains to determine the systems that serve for its accumulation. L-4=Azaleucine in radio-labeled form was synthesized and resolved by the action of hog kidney N-acylamino-acid amidohydrolase (EC 3.5.1.B) on the racemic alpha-N-acetyl derivative of DL-[... | 238,951 |
Purification and some properties of cathepsin A of small molecular size from pig kidney. | Cathepsin A [EC 3.4.2.-] of small molecular size (cathepsin A, S) has been purified about 800-fold from pig kidney by procedures including chromatographies on DEAE-Sephadex, SP-Sephadex, and Sephadex G-150. 1. The homogeneity of the purified enzyme was proved by ultracentrifugation and polyacrylamide gel electrophoresi... | 238,965 |
The formation of hydrogen peroxide during the oxidation of reduced nicotinamide adenine dinucleotide by cytochrome o from Vitreoscilla. | The formation of hydrogen peroxide during the oxidation of NADH by purified preparations of cytochrome o has been demonstrated by employing three independent methods: polarographic, colorimetric, and fluorometric. The first two methods were used to assay for the accumulation of hydrogen peroxide and showed that hydroge... | 238,973 |
Structural, kinetic, and renaturation properties of an induced hydroxypyruvate reductase from Pseudomonas acidovorans. | A hydroxypyruvate reductase has been induced in Pseudomonas acidovorans by growth on glyoxylate. The enzyme has been purified to homogeneity as assessed by the criteria of analytical ultracentrifugation and analytical disc gel electrophoresis. It has a molecular weight of approximately 85,000 and is composed of two ide... | 238,981 |
Permeability of muscle capillaries to small heme-peptides. Evidence for the existence of patent transendothelial channels. | Two heme-peptides (HP) of about 20-A diameter (heme-undecapeptide [H11P], mol wt approximately 1900 and heme-octapeptide [H8P], mol wt approximately 1550), obtained by enzymic hydrolysis of cytochrome c, were sued as probe molecules in muscle capillaries (rat diaphragm). They were localized in situ by a perixidase reac... | 239,003 |
Ligand-exchange chromatography of amino acids on nickel-Chelex 100. | A ligand-exchange chromatographic proceudre for the selective separation of amino acids from inorganic ions is presented. It was found that the binding of amino acids to the nickel-Chelex 100 resin is pH dependent. At pH 8.5-9.1, only the basic amino acids lysine, histidine and arginine are quantitatively attached to t... | 239,008 |
Interaction of amino acids, N-acetyl amino acid esters, thymine and adenine with sephadex LH-20 gel. | N-Acetyl aromatic amino acid esters, tryptophan, adenine and thymine show strong retention in Sephadex LH-20 gel in an aqueous phase. The decreased retention in 6 M urea, the absence of retention in absolute methanol and the increased retention at higher temperatures in the aqueous phase indicate that hydrophobic inter... | 239,013 |
The concentration of cerebrospinal fluid potassium during systemic disturbances of acid-base metabolism. | A possible relationship between blood acid-base state and the concentration of cerebrospinal fluid potassium has been examined in patients with systemic disturbances of acid-base metabolism. Over the range of values studied it was not possible to demonstrate any significant correlation between these parameters. | 239,024 |
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