title stringlengths 0 1.13k | abstract stringlengths 1 15.7k | PMID int64 22 36.5M |
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A study of the interaction between D-amino acid oxidase and quasi-substrates. | To study the interaction between D-amino acid oxidase [EC 1.4.3.3] and quasi-substrates such as benzoate and o-, m-, and p-aminobenzoate, visible circular dichroism spectra (CD spectra) were measured and the binding rate and affinity of o-aminobenzoate to the enzyme were observed by following the absorption changes at ... | 12,151 |
Metabolism of triacylglycerol in Mycobacterium smegmatis. | Mycobacterium smegmatis cells incorporated [1-14C]oleic acid into triacylglycerols (TG) from the medium more rapidly than shorter chain fatty acids, caprilic and butyric acids. This incorporation was inhibited more strongly by 10(-3) M N-ethylmaleimide than by 10(-3) M KCN. [14C]TG in the bacterial cells was utilized w... | 12,154 |
Activation of soluble guanylate cyclase from rat lung by incubation or by hydrogen peroxide. | A 37,000 X g supernatant fraction prepared from fat lung homogenate demonstrated a 2- to 3-fold increase in guanylate cyclase activity after incubation at 30 degrees for 30 min (preincubation). Treatment of the supernatant fraction with Triton X-100 increased activity to approximately the same extent as preincubation, ... | 12,160 |
Synthesis of bound adenosine triphosphate from bound adenosine diphosphate by the purified coupling factor 1 of chloroplasts. Evidence for direct involvement of the coupling factor in this "adenylate kinase-like" reaction. | Electrophoretically homogeneous coupling factor 1 from spinach chloroplasts binds ADP and converts the bound ADP to bound ATP and AMP. That this transphosphorylation of enzyme-bound ADP is catalyzed by the coupling factor itself, and not be a conventional adenylate kinase which might possibly contaminate preparations o... | 12,178 |
Comparison of the size and physical properties of gamma-glutamyltranspeptidase purified from rat kidney following solubilization with papain or with Triton X-100. | gamma-Glutamyltranspeptidase is associated with the brush border membrane of kidney proximal straight tubule cells. It can be solubilized qualitatively by treatment with papain or Triton X-100. Neither procedure affects its catalytic activity but the two resulting forms of the enzyme differ considerably in their physic... | 12,182 |
Plasma protein synthesis by isolated rat hepatocytes. | A system of preparation of rat hepatocytes with extended viability has been developed to study the role of hormones and other plasma components upon secretory protein synthesis. Hepatocytes maintained in minimal essential medium reduced the levels of all amino acids in the medium except the slowly catabolized amino aci... | 12,183 |
Inhibition of mammalian soluble guanylate cyclase activity by adenosine 5'-tetraphosphate, guanosine 5'-tetraphosphate and other nucleotides. | The effects of a variety of purine and pyrimidine nucleotides were tested for their capacity to inhibit mammalian soluble guanylate cyclase activity. Adenosine 5'-tetraphosphate (ATetP), ATP, ADP, AMP, guanosine 5'-tetraphosphate (GTetP) and GDP were found to inhibit soluble guanylate cyclase activity from rat lung and... | 12,193 |
Acetylcholine synthesis in sympathetic human neuroblastoma. | The synthesis of acetylcholine, as well as catecholamines, was studied by assaying the activities of choline acetyltransferase (ChA) and tyrosine hydroxylase (TH) in the tumor tissues and the culture cells of human neuroblastoma. In the majority of 20 neuroblastomas of sympathetic origin, both ChA and TH activities wer... | 12,256 |
The adhesion of film coatings to tablet surfaces--instrumentation and preliminary evaluation. | The strength of the adhesive bond between a film coating and a tablet surface has been studied using a specially designed tensile testing apparatus. The adhesion has been taken as the force required to remove the film from unit area of the tablet surface and has been shown to be dependent on the compression pressure us... | 12,264 |
[Arteritis of the large vessels originating at the aorta (Takayasu arteritis) in childhood (author's transl)]. | A 9-month-old female child with extensive inflammatory lesions in the large arterial trunks originating at the aorta (Takayasu arteritis) was reported. Severe cerebral, intestinal and coronary circulatory disturbances as well as insufficient blood supply in both arms, particularly the left arm, developed as a result of... | 12,403 |
[Cyclic AMP and plasma renin activity in renal vein blood after amitryptiline, theophylline, furosemide and beta adrenergic blocking substances (author's transl)]. | The influence of amitryptiline, theophylline and furosemide on the concentration of cyclic-AMP and plasma renin activity (PRA) was investigated in renal vein plasma. Additionally, the stimulating effect of furosemide on the PRA after application of the beta-adrenergic receptor antagonists propranolol and practolol and ... | 12,404 |
[The effect of surgery, performed under ischemia, on the fine structure of the hand muscles]. | The ultrastructural picture of the intact and injured hand muscles has been examined by the authors during and after operations in tourniquet ischaemia of 12 patients, in connection with tendon and nerve reconstruction. Muscle specimina were taken partly in the beginning of the ischaemization, and partly at the end ot ... | 12,410 |
Immunohistochemical studies on the localization and distribution of monoamine neuron systems in the rat brain. I. Tyrosine hydroxylase in the mes- and diencephalon. | The localization and distribution of tyrosine hydroxylase (TH), the first enzyme in the catecholamine synthesis, in the mes- and diencephalon has been studied with the indirect immunofluorescence technique of Coons and collaborators. Principally, TH was present in neuron systems with a distribution similar to known dop... | 12,423 |
Adding a Medex to the medical mix: an evaluation. | Three classes of Medex were followed during their preceptorship training and subsequent employment to assess the impact of adding such physician assistants on 13 private practices. The data suggest that the Medex are readily incorporated into the practice, seeing a similar spectrum of patients and generally functioning... | 12,425 |
[Biosynthesis of chitinase by Achromobacter liquefaciens]. | Bacterial cultures under study synthesize exocellular chitinase on a medium containing chitin or demineralized crab shells as a source of carbon and nitrogen. Conditions for biosynthesis of chitinase by the cells of Achromobacter liquefaciens 301a were investigated under periodic and continuous conditions of cultivatio... | 12,452 |
New approaches to the causation and prevention of cancers of epithelial surfaces. | Environmental rather than genetic factors appear to be responsible for the causation of cancers involving epithelial surfaces, especially oropharyngeal cancers, cancers of the gastro-intestinal tract, cancers of the liver and gall-bladder, cancers of the cervix uteri and lung cancers. Epithelial surfaces which have muc... | 12,456 |
Phenylalanine hydroxylase and tyrosine aminotransferase in human fetal and adult liver. | The mean value of phenylalanine hydroxylase activity in adult human liver was almost twice as high as that in the fetal ones; no consistent variations were seen with sex, fetal age (between the 11th and 22nd week of gestation), or with hours between delivery and death. The tyrosine aminotransferase levels did not corre... | 12,495 |
Failure of parathyroid hormone and cyclic AMP to inhibit renal carbonic anhydrase. | It has been suggested that the parathyroid hormone and cyclic AMP produce their bicarbonaturic effects through inhibition of renal carbonic anhydrase. In the present study, the incubation of renal carbonic anhydrase with parathyroid hormone or cyclic AMP in presence of ATP, Mg++ and K+ ions, did not produce any inhibit... | 12,496 |
Implications of electrostatic potentials on ribosomal proteins. | Potentiometric studies of ribosomal particles 30S, 50S, and 70S, were designed to investigate possible implications of the electrostatic potentials developed by the 16S and 23S rRNA fractions. Release of protons and proton titrations of these ribosomal fractions were examined as a function of Mg2+ and K+ concentrations... | 12,498 |
Effects of pyrroxan and chlordiazepoxide on biogenic amine metabolism in the rat brain. | Pyrroxan (20 mg/kg, i.p.), a new potential antianxiety agent, increased brain norepinephrine (NE) turnover in rats, reflecting a possible central alpha-adrenergic receptor blocking activity. In contrast, chlordiazepoxide (20 mg/kg, i.p.), a widely used antianxiety agent, did not alter the NE turnover. Pyrroxan did not ... | 12,528 |
Stages of bone marrow transplantation: a psychiatric perspective. | Patients undergoing bone marrow transplantation were closely followed by a psychiatric service functioning as part of a multidisciplinary team. A somewhat predictable pattern of psychological reactions to the stress of various stages of the procedure appeared to emerge. Approaches to patient evaluation, typical patient... | 12,531 |
Participation of leukemia cells in immune responses. | Cells of a transplantable lymphoid leukemia of mice were tested in vivo and in vitro to see which features of normal lymphoid cells were retained in spite of malignant transformation and lack of growth control. Leukemia cells phagocytosed, adhered to glass, possessed receptors for immunoglobulin, participated in the im... | 12,544 |
Microcalorimetric measurements of heat production in human erythrocytes. III. Influence of pH, temperature, glucose concentration, and storage conditions. | Heat production in human erythrocytes has been measured under different conditions of pH, glucose concentration, and temperature. Storage conditions have also been varied. The erythrocytes, which were from healthy subjects, were suspended either in autologous plasma or in phosphate buffer. The heat effect, P, was shown... | 12,557 |
Acid-base determinations in normal and asphyxiated term infants during the first 24 hours of life. | Vigorous term infants who are born normally initially have a moderate metabolic and respiratory acidosis which is corrected by the time the infant is 6 hours old. Asphyxiated infants consistently show a greater degree of acidosis, both metabolic and respiratory. Caesarean section results in higher pCO2 values, especial... | 12,578 |
Chemical studies of marine invertebrates. XXIII. A novel polyhydroxylated sterol from the soft coral Litophyton viridis (Coelenterata, Octocorallia, Alcyonacea). | The structure of 24-methylenecholest-5-en-3beta,7beta,19-triol (II) isolated from the soft coral Litophyton viridis, has been established by X-ray diffraction analysis. The compound is accompanied by its 7-monoacetate derivative. | 12,598 |
Induction of increased graft-versus-host disease by mouse spleen cells sensitized in vitro to allogeneic tumor. | The aim of our study was to sensitize cells in vitro, follow their proliferative and cytotoxic responses, and determine their ability to cause lethal graft-versus-host disease (GVHD). C57BL/6 (H2b) spleen cells were incubated with irradiated BALB/C (H2d) Moloney lymphoma cells (LSTRA) in mixed leukocyte culture conditi... | 12,600 |
Hyperlactatemia and hemolysis in G6PD deficiency after nitrofurantoin ingestion. | A 69-year-old man with glucose-6-phosphate dehydrogenase deficiency was treated with nitrofurantoin for pyuria. Four days later he presented with metabolic acidosis due to excess lactic acid, a decline in curculating hemoglobin, reticulocytosis, elevated serum transaminase levels, and hyperbilirubinemia. The drug was w... | 12,659 |
[Salvage of a patient with respiratory distress caused by tracheal obstruction, by use of a Fogarty catheter]. | The author observed a state of acute asphyxia due to a mucosal plug in a patient undergoing total laryngectomy. After failure of classical methods of removal and the plug and in view of the critical state of the patient, a heroic procedure was used: extraction of the mucosal plug using a Fogarty catheter, as used in ar... | 12,693 |
[Difficult intubations and proposed solutions. Intubation by fiberscopy]. | After a review of the various techniques used in difficult intubations, the authors emphasize recent progress in the field of fiber endoscopy. The advantages of this technique, e.g. absence of trauma, increased field of exploration and reduction in dose of anaesthetic drugs, exceed the present disadvantages, e.g. high ... | 12,701 |
[Sterilization of intratracheal tubes]. | After a brief review of classical and modern methods of sterilisation of endo-tracheal catheters, where they emphasize the dangers of the use of ethylene oxide, the authors recall the common procedure which they used: washing with water and soap, and soaking for 6 to 7 hours in formaldehyde or in a solution of quaterna... | 12,705 |
[Glycosyltranferases. Biological and pathological importance]. | Catalysing the transfer of oses and ose derivatives on various accepters, glucosyltransferases are of increasing importance in biology and pathology. Their sub-cellular localisation is now known together with the physico-chemical parameters of their activity and the importance of polyprenic intermediates. The first phe... | 12,708 |
Diffusion coefficients for protein molecules in blood serum. | A new technique is described for the measurement of the self-diffusion coefficients of protein macromolecules in solution. The method makes use of the phenomenon of Taylor dispersion of a solute introduced into a solvent flowing in the laminar regime through a tube of circular section. Results are reported for the self... | 12,775 |
Characterization of the acetyl-CoA synthetase of Acetobacter aceti. | The acetate activating system of Acetobacter aceti has been studied. The enzyme responsible, acetyl-CoA synthetase, has been purified about 500-fold from crude cell extracts and was approximately 85% pure as judged by polyacrylamide gel electrophoresis in sodium dodecyl sulphate. The purified enzyme showed optimal acti... | 12,800 |
Thermoplasma acidophilum: intracellular pH and potassium concentration. | Thermoplasma acidophilum is a free-living thermophilic mycoplasma. Although the organism lacks a cell wall, it can grow in medium as dilute as 66 mosM. The intracellular K+ concentration can be as low as 17 mM, but varies according to the osmolality of the culture medium. The internal pH can be measured by taking advan... | 12,804 |
Influence of 8-substitutes on the oxidation of hypoxanthine and 6-thioxopurine by bovine milk xanthine oxidase. | 1. The influence of 8-substituents was studied on the rate of oxidation of hypoxanthine and 6-thioxopurine by bovine milk xanthine oxidase (EC 1.2.3.2). 2. An 8-methyl group does not alter the rate of oxidation of hypoxanthine materially, but an 8-phenyl substituent reduces it markedly. This is ascribed to inhibition o... | 12,828 |
Purification and properties of acid phosphatases isolated from Owenia fusiformis. | Acid phosphatase (EC. 3.1.3.2) has been separated by molecular sieving into two fractions and these fractions were purified by Sephadex ion-exchange chromatography. One of the purified enzymes (fraction II) was purified 830 fold and had a specific activity of 34 international units per mg protein at 37 degrees C and at... | 12,839 |
Looking both ways. Presidential address delivered at British Institute of Radiology Annual Congress-9th April 1976. | The address looks backward over the progress made in the techniques of radiology--both diagnosis and therapy--over the past 30 years and forward to the possibilities which lie ahead. It draws attention to the continuing need for basic research as the source from which practical advances spring, illustrating this from t... | 12,849 |
New sugars from antigenic lipopolysaccharides of bacteria: identification and synthesis of 3-O-[(R)-1-carboxyethyl]-L-rhamnose, an acidic component of Shigella dysenteriae type 5 lipopolysaccharide. | A new acidic sugar, 3-O-[(R)-1-carboxyethyl]-L-rhamnose (1), has been identified as a constituent of the O-antigenic lipopolysaccharide of Sh. dysenteriae type 5. The structure of 1 has been established by physico-chemical methods and by synthesis. Alkylation of methyl 2,5-di-O-benzyl-alpha-L-rhamnofuranoside (6) with ... | 12,866 |
Enzymatic assay of total cholesterol in serum or plasma by amperometric measurement of rate of oxygen depletion following saponification. | A method for serum or plasma cholesterol assay involving amperometric measurement of the rate of oxygen depletion in the cholesterol oxidase-catalyzed oxidation of cholesterol is described. The hydrolysis of the serum cholesterol esters is accomplished by saponification of 50 mul of sample with 0.2 ml of ethanolic KOH ... | 12,891 |
Translation of mRNA from rat-liver polysomes into tyrosine aminotransferase and tryptophan oxygenase in a protein-synthesizing system from wheat germ. Effects of cortisol on the translatable levels of mRNA for these two enzymes. | Messenger RNA was isolated from rat liver polysomes by phenol/chloroform extraction and subsequent oligo(dT)-cellulose chromatography. The mRNA was translated in a protein-synthesizing system in vitro derived from wheat germ. The system was optimized in respect to Mg2+ and K+. The presence of spermidine or spermine is ... | 12,944 |
Biosynthesis of peptidoglycan in Gaffkya homari. The incorporation of peptidoglycan into the cell wall and the direction of transpeptidation. | Wall membrane enzyme preparations from Gaffkya homari catalyze the formation of peptidoglycan from the precursor pairs: UDP-N-acetylglucosamine + UDP-N-acetylmuramyl-pentapeptide (UDP-MurNAc-Ala-DGlu-Lys-DAla-DAla) and also from UDP-N-acetylglucosamine + UDP-N-acetylmuramyl-tetrapeptide (UDP-MurNAc-Ala-DGlu-Lys-DAla). ... | 12,946 |
Efficient purification and molecular properties of spinach chloroplast fructose 1,6-bisphosphatase. | A relatively straightforward procedure has been developed for the purification of chloroplast fructose bisphosphatase from spinach leaves to apparent homogeneity and with 80% yield. The molecular weight of the enzyme was about 160 000. Chloroplast fructosebisphosphatase consists of four possibly identical subunits and,... | 12,949 |
Studies on 3-deoxy-d-arabinoheptulosonate-7-phosphate synthetase(phe) from escherichia coli k12. 3. Structural studies. | 1. Investigations with structural analogues of phenylalanine indicated an absolute requirement for the aromatic ring and both the alpha-carboxyl and alpha-amino groups of phenylalanine for inhibition of 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase(phe) activity. Replacement of the alpha-H atom with a methyl gr... | 12,953 |
Electron spin resonance investigations of mitochondrial electron transport in Neurospora crassa. Characterization of paramagnetic intermediates in a standard strain. | 1. Submitochondrial particles from Neurospora strain inl-89601 have been analyzed by electron spin resonance spectroscopy (ESR). Numerous signals due to iron-sulfur proteins are observed at low temperatures. Analysis of these ESR signals at various temperatures allows the assignment of resonances to iron-sulfur centers... | 12,965 |
Cyclic nucleotide hydrolysis in the thyroid gland. General properties and key role in the interrelations between concentrations of adenosine 3':5'-monophosphate and guanosine 3':5'-monophosphate. | Phosphodiesterase activities of horse (and dog) thyroid soluble fraction were compared with either cyclic AMP (adenosine 3':3'-monophosphate) or cyclic GMP (guanosine 3':5'-monophosphate) as substrate. Optimal activity for cyclic AMP hydrolysis was observed at pH 8, and at pH 7.6 for cyclic GMP. Increasing concentratio... | 12,974 |
Comparison of adiphenine and TRH effects on TSH release by rat pituitary in vitro. | The mechanism of action of adiphenine on in vitro rat anterior pituitary TSH release was compared to that of the physiological stimulator TRH. The comparative study showed that adiphenine and TRH were able to increase TSH release in a dose-dependent manner, had similar time courses of action for equipotent stimulating ... | 12,985 |
A comparison of laparoscopy and culdoscopy for internal sterilization. | In recent years, the increased demand for sterilization by women who have achieved their desired family size has emphasized the need to improve both existing methods of tubal occlusion and the means of access to the Fallopian tubes. Utilization of diagnostic instruments such as the laparoscope and culdoscope to perform... | 13,007 |
Ritodrine hydrochloride induced cardiopulmonary and placental hemodynamic changes in normal and hypertensive late pregnancy. | Cardiopulmonary parameters (minute volume, heart rate, stroke volume, right heart, pulmonary and left heart blood volumes), blood pressure and placental blood flow were evaluated in 20 normal patients, nine patients with preeclamptic disease and 12 with essential hypertension in late pregnancy before and 60 minutes aft... | 13,017 |
Terminal deoxynucleotidyl transferase as a biological marker for human leukemia. | High levels of terminal deoxynucleotidyl transferase have been observed in leukocytes of 7 out of 20 patients with chronic myelogenous leukemia in acute blast phase of the disease. These levels are comparable to the levels observed in human and calf thymus gland and cell lines with some T cell characteristics (Molt 4 a... | 13,027 |
Role of urinary solutes in natural immunity to gonorrhea. | Natural resistance of the male urethra to gonococci has not been explained by classical immune mechanisms but could result from antibacterial properties of urine. Accordingly, we measured survival in midmorning urine of 10(7) F-62 T2 gonococci per ml by serial dilutions and plate counts. Fifteen killer urines from eigh... | 13,038 |
Adenosine triphosphate catabolism in homogenates of rat secretory enamel organs incubated in histochemical lead media. | To investigate how lead, when used as trapping agent, influences the ATP hydrolysis and to study how ATP is catalyzed in histochemical systems, homogenized secretory enamel organs were incubated in histochemical [3H]-ATP media. Aliquots from the media were taken after 3, 10, 20 and 30 min, the ATP and formed metabolite... | 13,054 |
Rhodanese from Thiobacillus A2: catalysis of reactions of thiosulphate with dihydrolipoate and dihydrolipoamide. | Rhodanese (thiosulphate:cyanide sulphurtransferase EC.2.8.1.1) was purified 25- to 30-fold from thiosulphate-grown Thiobacillus A2. It exhibited a pH optimum between pH 10-2 and 10-4 and apparent Km values of 0-36 mM-Na2S2O3 and 17 mM-KCN. Ultraviolet spectrophotometry and thin-layer chromatography showed that the enzy... | 13,142 |
Regulation of the tricarboxylic acid cycle and poly-beta-hydroxybutyrate metabolism in Azotobacter beijerinckii grown under nitrogen or oxygen limitation. | Azotobacter beijerinckii was grown in ammonia-free glucose/mineral salts media in chemostat culture under oxygen or nitrogen limitation. Selected enzymes of the tricarboxylic acid cycle and poly-beta-hydroxybutyrate metabolism were monitored in relation to oxygen supply for both steady and transition states. Two dissol... | 13,143 |
The effect of C.P.A.P. upon pulmonary reserve and cardiac output under increased abdominal pressure. | The cardiopulmonary effects of CPAP under conditions of increased intra-abdominal pressure were studied using the rabbit as an experimental model. Central venous pressure and cardiac output were reduced when the intra-abdominal pressure was increased. Addition of CPAP caused a further reduction in C.V.P. and C.O. Despi... | 13,172 |
[Treatment of ischemic heart disease with "coronary drugs" (author's transl)]. | The standard treatment of ischemic heart disease consists in the administration of nitrates and beta-sympathicolytic agents. Calcium antagonists are likewise promising. They should be used particularly if beta-sympathicolytic agents are contraindicated. In the beginning special caution should be exercised on combining ... | 13,298 |
Preponderance of beta adrenergic receptors in the gastric fundus of the rat. | There are two kinds of adrenergic receptors in rat fundus (stomach), the alpha receptors are stimulated with adrenaline and noradrenaline and can be blocked effectively with azapetine and the beta receptors are stimulated with isoprenaline and adrenaline and can be blocked effectively with propranolol. Adrenaline, nora... | 13,339 |
Recommending coronary artery surgery: refining judgement through application of new knowledge. | An analysis is made to help decide on the best form of treatment for atherosclerotic coronary disease. Available data on surgical treatment are reviewed with emphasis on prognosis and management of the patient with stable angina. | 13,356 |
Rhodopsin in model membranes: charge displacements in interfacial layers. | A model membrane was developed in which interfacial layers of rhodopsin were reoriented onto one side of a thin Teflon film separating two aqueous compartments. Flashes evoked fast photoelectric signals (1 ms) that originated from capacitative charge displacements of oriented rhodopsin upon bleaching. The photoelectric... | 13,363 |
Intramolecular general base-catalyzed ester hydrolyses by the imidazolyl group. | Intramolecular general base catalysis by the imidazolyl group was found in the hydrolyses of endo-5-[4;(5')-imidazolyl]-bicyclo[2.2.1]hept-endo-2-yl trans-cinnamate and endo-5-[4'(5')-imidazolyl]bicyclo[2.2.2]oct-endo-2-yl trans-cinnamate in which the imidazolyl and trans-cinnamoyl groups are bound in close proximity t... | 13,364 |
Inhibitors of genetic recombination in pneumococci. | Treatment of transformable pneumococci with DNA-intercalating agents shortly after the uptake of DNA molecules inhibited the appearance of genetic transformants. The same drug treatments applied 20 min after DNA uptake were ineffective. Ethidium bromide, proflavin, daunomycin, actinomycin D, and platinum red were found... | 13,367 |
Effects of L-prolyl-L-leucyl-glycine amide (MIF-I) on dopaminergic neurons. | In an attempt to determine the mechanism of action of L-proly-L-leucyl-glycine amide (MIF-I) in the treatment of Parkinson's disease, various parameters of dopaminergic neuronal function were studied in rats. It was found that the active uptake of 3H-dopamine (3H-DA) by synaptosome-rich homogenates of the striatum of r... | 13,412 |
Hypothalamic releasing factors: physiological evidence for a regulatory action on central neurons and pathways for their distribution in brain. | Recent observations based on single cell recordings obtained in various areas of the brain indicate that TRH, LH-RH and somatostatin have potent effects on the activity of central neurons. There is also electrophysiological evidence for the existence of a system of hypothalamic tuberoinfundibular neurons with widesprea... | 13,416 |
Assembly of a spherical plant virus. | The conditions previously reported as necessary for the reassembly of spherical viruses have been distinctly unphysiological and such reassembly cannot be related directly to the in vivo reaction. Mild conditions for the in vitro reassembly of cowpea chlorotic mottle virus (CCMV) from its isolated components have now b... | 13,422 |
Physiological viewpoints on clinical acid-base diagnostics. | Acid-base physiology is concerned with sources, extent, and control of hydrogen ion donation in the body, at the organ-physiological as well as the molecular level of study. With the introduction of Van Slyke's methods for quantitative carbon dioxide measurements in biological fluids, one important source of hydrogen i... | 13,479 |
A review of recent advances in vascular smooth muscle pharmacology. | An understanding of the physiology of vascular smooth muscle and knowledge of the reaction of such muscle to certain drugs are essential for the development of a means of treating cerebral arterial spasm. The role of cyclic nucleotides in vascular smooth muscle activity is reviewed and possible therapeutic approaches a... | 13,504 |
Arterial blood gas tensions, hydrogen ion, and electroencephalogram during sleep in patients with chronic ventilatory failure. | We have studied arterial PO2, PCO2, and hydrogen ion and electroencephalogram during sleep in 10 patients with stable severe chronic respiratory failure. As a group the patients slept badly. Sleep was associated with a worsening of hypoxia and no significant change in PCO2 and H+. Two patients were restudied, receiving... | 13,511 |
[Histopathological study of the mosquito-bite infiltrate in patients with various underlying diseases and different medications]. | A histologic study was carried out on tissue-reactions after insect bits in 9 patients with various primary diseases. The clinical and cellular reactions to the bite in the epidermis varied, according to the length, intensity of toxity of the insect bit. The dermal infiltrate was similar in almost all cases and corresp... | 13,547 |
[Comparison in vitro between farm-yard manure and liquid manure (author's transl)]. | Spreading of liquid manure in a grassland ecosystem, stable since a long time when treated with farm-yard manure, brings out a great unbalance in the soil. In comparison of farm-yard manure, the liquid manure involves -- a decrease of C and N in the soil, -- a deterioration of the soil structure, -- a dangerous change ... | 13,589 |
Histochemical studies on the mucins of the vertebrate tongues. VII. Histochemical analysis of mucosubstances in the tongues of some fishes. | The tongues of three fishes were investigated histochemically to determine the distribution and nature of mucosubstances by employing recent techniques and the results were considered comparatively with the lingual mucosubstances of other vertebrates. A heterogenous distribution of neutral mucosubstances, sulfomucins a... | 13,596 |
Comparative evaluation of neuromuscular blockade and reversibility of AH 8165 and pancuronium bromide in man. | The neuromuscular properties of AH 8165 were compared to those of pancuronium bromide in 66 patients during analgesic anesthesia procedures. Administration of 0.5 mg/kg AH 8165 and 0.04 mg/kg pancuronium bromide produced very similar degrees of non-depolarizing block. AH 8165 acted quicker and therefore gave better con... | 13,601 |
Reduced inactivation of tyrosine aminotransferase in the prefused rat liver in the presence of ethanol. | The mode of action whereby alpha-methyltyrosine (alpha-MT) potentiates the behavioural effects induced by catecholamine receptor blocking antipsychotic drugs was investigated in rats trained to lever-press for food on a fixed-ratio 40 schedule of reinforcement. It was found that alpha-MT (20 mg/kg intraperitoneally -- ... | 13,604 |
Cholinergic mechanisms in the learning and memory facilitating effect of caffeine. | Experiments are made to train rats in a maze involving memory tests 24 hours and 14 days after training. As it was established in previous studies of the authors, caffeine introduced in low doses (5 mg/kg) 5 min before training facilitates learning and memory. Applied immediately after training, caffeine even in higher... | 13,607 |
Phenothiazine-induced alterations of immune response in experimental tick-borne encephalitis: morphological model analysis of events. | The depressive effect of trifluoperazine (TFP), a phenothiazine derivative, on the morphology of the development of immune response (IR) (humoral and cell-mediated component) was studied in mice given tick-borne encephalitis (TBE) virus, sheep red blood cells (SRBC) or the BCG vaccine. This effect was manifested by a d... | 13,643 |
Effect of methylated PGE2 analogs given orally on pancreatic response to secretin in man. | The effect of two methylated PGE2 analogs given orally on the pancreatic response to intravenous secretin has been studied in 8 healthy subjects. The secretion of bicarbonate was not changed by these PGE2 analogs. The secretion of enzymes during infusion of PGE2 analogs was significantly greater than in the control. | 13,652 |
Physiologic effects of normal-or low-oxygen-affinity red cells in hypoxic baboons. | Baboons were bled one-third their red cell mass and were given homologous transfusions of red blood cells to restore the red cell volume. One group of baboons received red blood cells with a normal 2,3-diphosphoglycerate 2,3-DPG) level and normal affinity for oxygen, and in this group the 2,3-DPG level after transfusio... | 13,664 |
The effect of adrenergic receptor blocking agents on arrhythmia caused by trauma. | Experiments on animals have shown that blunt trauma to the anterior chest wall causes arrhythmia and conduction disturbances. In most cases nodal rhythm and ST segment elevation were observed. Microscopic examination disclosed myocardial damage in the vicinity of the atrioventricular node. Administration of alpha or be... | 13,678 |
Control of breathing using an extracorporeal membrane lung. | Various amounts of carbon dioxide were removed through an extracorporeal membrane lung in spontaneously breathing lambs. The decrease in alveolar ventilation was proportional to the fraction of total carbon dioxide removed by the membrane lung. When extracorporeal CO2 removal approximated CO2 production (VCO2), alveola... | 13,683 |
[Endocrine effects of neuroleptics]. | The neuroleptic drugs used in anesthetics belong to the group of phenothiazines or butyrophenones. The endocrine response to their intravenous administration is still ill-known and usually only concerns the association of anesthetics and neuroleptics. However, as far as the catecholamines are concerned, it is known tha... | 13,691 |
Some aspects of the persistence and fate of acrolein herbicide in water. | Experimental data for the decay of acrolein approximated first order kinetics. The reaction continued to completion in local waters but in buffered solution (pH 5.1-8.6) an equilibrium was reached after reaction of about 92% of the acrolein. It is proposed that data presented on the effects of pH on decay of acrolein m... | 13,740 |
Energetic aspects of anaerobic growth of Aerobacter aerogenes in complex medium. | Molar growth yields for anaerobic growth of Aerobacter aerogenes in complex medium were much higher than for growth in minimal medium. In batch cultures the molar growth yield for glucose varied from 44 to 50 and YATP from 17.1 to 18.8. For glucose-limited chemostat cultures a value of 17.5 g/mole was found for Y max A... | 13,757 |
Ammonium uptake and metabolism by mitrogen fixing bacteria. II. Klebsiella pneumoniae. | The primary steps of N2, ammonia and nitrate metabolism in Klebsiella pneumoniae grown in a continuous culture are regulated by the kind and supply of the nitrogenous compound. Cultures growing on N2 as the only nitrogen source have high activities of nitrogenase, unadenylated glutamine synthetase and glutamate synthas... | 13,759 |
Monoamine metabolism in human brain. | Norepinephrine (NE), dopamine (DA), tyrosine hydroxylase (TH), catechol-O-methyltransferase (COMT) and monoamine oxidase (MAO) levels were measured in human brain tissue obtained at autopsy from a series of 39 patients dying of various medical and accidental causes. The nine following brain areas were studied: globus p... | 13,761 |
Validation of a simple radiochemical assay measuring hydrolysis of choline-labelled microsomal phosphatidylcholine by phospholipase C. pH-dependence. | Selective hydrolysis of phosphatidylcholine species, which are selectively radioactively labelled in vivo, does not appear to interfere with a radiochemical assay for hydrolysis of microsomal phosphatidylcholine by C-type phospholipases from Bacillus cereus or Clostridium perfringens. Both phospholipases substantially ... | 13,785 |
Purification and properties of debranching enzyme from dogfish muscle. | Glycogen debranching enzyme (4-alpha-glucanotransferase amylo-1,6-glucosidase, EC 2.4.1.25 + 3.2.1.33) was purified 140-fold from dogfish muscle in a rapid, high-yield procedure that takes advantage of a strong binding of the enzyme to glycogen, and its quantitative adsorption to concanavalin A-Sepharose only when the ... | 13,809 |
Cation transport in cytochrome oxidase reconstituted vesicles. | Cation translocation across the membrane of cytochrome oxidase reconstituted vesicles may be followed with a simple spectrophotometric method. Cytochrome oxidase reconstituted vesicles, supplemented with ascorbate and cytochrome c. induce large spectral changes of the positive dye safranine, reversed by uncouplers and ... | 13,827 |
Bis-(4-methylumbelliferyl) phosphate as a substrate for the surface membrane-associated phosphodiesterase activity of pig platelets. | It was found that the newly-available compound, bis-(4-methylumbelliferyl) phosphate, could be used as a substrate for the pig platelet surface membrane-associated phosphodiesterase activity, usually assayed with bis-(p-nitrophenyl) phosphate. This enzyme activity is distinct from the phosphodiesterase activity towards... | 13,836 |
A specific polyadenylase from Escherichia coli K12. | A polyadenylase, degrading specifically poly(A) sequences was isolated from Escherichia coli K12. The enzyme was purified about 850 times to practically electrophoretic homogeneity. It was free of poly(A) polymerase activity, as well as of the well known E. coli RNAases I and II. It is stimulated by bivalent cations li... | 13,837 |
Purification and properties of aldehyde dehydrogenase from Proteus vulgaris. | NADP-linked aldehyde dehydrogenase (aldehyde : NADP+ oxidoreductase, EC 1.2.1.4) was purified from Proteus vulgaris to the stage of homogeneity as judged by ultracentrifugation and polyacrylamide gel electrophoresis. The molecular weight of the purified enzyme was estimated to be 130000 by gel filtration. The enzyme wh... | 13,839 |
Purification and properties of one component of acid phosphatase produced by Aspergillus niger. | One component, the i form, of acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) produced by Aspergillus niger was purified from the mycelial extract. The purified enzyme was homogenous on Sephadex G-200 gel filtration, disc electrophoresis and heat inactivation. The purified enzym... | 13,843 |
The transsulfuration pathway in Tetrahymena pyriformis. | Four enzymes necessary for the metabolism of methionine by the trans-sulfuration pathway, methionine adenosyltransferase (EC 2.5.1.6), adenosylhomocysteinase (EC 3.3.1.1), cystathionine beta-synthase (EC 4.2.1.22) and cystathionine gamma-lyase (EC 4.4.1.1) were identified in Tetrahymean pyriformis. The ability of these... | 13,863 |
Inactivation of mitochondrial 2-oxoglutarate dehydrogenase complex as a result of phospholipid degradation induced by freeze-thawing. | The inactivation of 2-oxoglutarate dehydrogenase complex by freeze-thawing was examined along with alterations of membrane phospholipids, in order to elucidate the mechanism of freezing injury in mitochondria. The dehydrogenase complex activity in slowly frozen and thawed mitochondria decreased to 70% as compared to in... | 13,867 |
The molecular dynamics of hyaluronates in solution. | The dynamic properties of hyaluronate solutions are discussed with relevance to some problems in sensory physiology (mechanoelectrical transduction), renal physiology, interstitial fluid regulation, and especially to the causes of open-angle glaucoma. With respect to the last problem: as recent biochemical evidence ind... | 13,876 |
[Effect of hypoxia on the concentration of nicotinamide coenzymes in the tissues of newborn rats]. | The content of nicotinamide coenzymes (NAD, NAD-H, NADP, NADP-H) was studied in the brain, heart and liver tissue of the newborn rats kept in hypoxic gaseous medium with a 4% oxygen content for 2 1/2 hours. There was a marked reduction of NAD content, an accumulation of NAD-H and a more than two-fold fall of the NAD/NA... | 13,878 |
Influence of oxygen and carbon dioxide on the plasma-erythrocyte pH relationship in normal human whole blood. | The influence of oxygenation level (oxyhaemoglobin saturation 0 or 100%) on the relationship between plasma pH and erythrocyte pH was studied, in vitro, in normal human blood submitted to changes in carbon dioxide tension. Firstly, the pH of both true and separated erythrolysates were compared: for the former, tonometr... | 13,893 |
The normal occurrence of octopamine in the central nervous system of the rat. | An enzymatic assay for octopamine capable of detecting 50 pg of amine was developed and used to study the distribution of octopamine in regions of the rat central nervous system. The presence of octopamine in the rat pineal organ was confirmed by mass spectrometry; Administration of a monoamine oxidase inhibitor and of... | 13,909 |
Ganglioside GM2 N-acetyl-beta-D-galactosaminidase and asialo GM2 (GA2) N-acetyl-beta-D-galactosaminidase; studies in human skin fibroblasts. | Ganglioside GM2 and its asialo-derivative, GA2 were radiolabeled in their N-acetyl-D-galactosaminyl moieties by oxidation with galactose oxidase and reduction with tritiated sodium borohydride. Specific activities of 6 X 10(4) dpm/nmol (GM2) and 1.8 X 10(6) dpm/nmol (GA2) were achieved. About 98% of the label was in N-... | 13,950 |
Altered hepatic blood flow and drug disposition. | For some drugs, delivery to the liver by the hepatic circulation is an important determinant of removal by this organ. Classical pharmacokinetic analyses cannot predict the changes produced by altering any of the biological determinants of drug elimination by the liver; hepatic blood flow, metabolic enzyme activity, dr... | 13,954 |
Monoamine metabolites in cerebrospinal fluid and serotonin uptake inhibition during treatment with chlorimipramine. | The effects of chlorimipramine on the concentrations of the main metabolites of serotonin (5-HT) norepinephrine (NE), and dopamine, i.e. 5-hydroxyindoleacetic acid (5-HIAA), 4-hydroxy-3-methoxyphenyl glycol (HMPG) and homovanillic acid (HVA), respectively, were studied in cerebrospinal fluid from 14 depressed patients,... | 13,959 |
Effect of ultrasonic nebulization on arterial oxygen saturation in chronic obstructive pulmonary disease. | Twenty patients with mild to severe chronic obstructive pulmonary disease received ultrasonic nebulization to assess the danger of short-term changes in blood gas levels during this therapy. The status of arterial oxygenation was monitored during 20 minutes of therapy and for 20 minutes following therapy. In nine patie... | 13,968 |
Controlled comparison of bromazepam, amitriptyline, and placebo in anxiety-depressive neurosis. | Seventy-two private practice patients with moderate or severe anxiety-depressive neurosis received mean daily oral doses of 23.8 mg bromazepam, 94 mg amitriptyline, or 4.6 capsules of placebo in a double-blind four-week study. The patient's condition was assessed initially and at weekly intervals by using the Brief Ps... | 13,969 |
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