title stringlengths 0 1.13k | abstract stringlengths 1 15.7k | PMID int64 22 36.5M |
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Monkeypox-specific antibodies in human and simian sera from the Ivory Coast and Nigeria. | A test for monkeypox-specific antibodies is described. Monkeypox immune sera can be made type-specific by immunoabsorption with heterotypic poxvirus extracts. Monkeypox-specific antibodies were demonstrated in sera from 9 cynomolgus monkeys (Macaca fascicularis) that had previously been experimentally infected with mon... | 186,210 |
[Statistical relationship between "slow-wave sleep" and "paradoxical sleep" in rats]. | In the Rat, statistical analysis of slow sleep (SS) and paradoxical sleep (PS) episodes disclosed two significant (p less than 0,01) positive correlations: (1) Over a four-hour period, the mean duration of PS episodes was correlated to the mean of the just preceding "light" SS episodes; (2) On the contrary, from cycle ... | 186,214 |
Regulation of active ion transport in the small intestine. | The epithelium of the small intestine can both actively absorb and actively secrete electrolytes and water. Secretion can be elicited in vitro by adding cyclic AMP or a stimulator of intestinal mucosal adenylate cyclase (cholera and Escherichia coli enterotoxins, prostaglandins, vasoactive intestinal peptide) or an inh... | 186,235 |
Recent advances in the aetiology of viral gastroenteritis. | Studies with the human reovirus-like (HRVL) agnet, also designated rotavirus and duovirus, have revealed that it is a major aetiological agent of diarrhoea of infants and young children in many parts of the world. In a study of patients admitted with a diarrhoeal illness to the Children's Hospital of the District of Co... | 186,239 |
Weakening of genetic resistance. II. The effect of injection of the macrophage toxic agents silica and carragheenan and the cytostatic drugs cyclophosphamide, busulphan and vinblastin. | Silica (4 mg/i.v.) and Carragheenan (20 mg/i.p.) were shown to be capable of weakening hybrid resistance in the spleen whereas no improved growth was observed in the femoral bone marrow. Histological data did not indicate that the agents in the doses used caused significant macrophage mortality. It was concluded that t... | 186,293 |
[Effect of catecholamines on sodium resorption and oxygen tension in rat kidneys]. | Functional significance of alpha- and beta-adrenoreceptors for the mechanism of catecholamines effect on sodium reabsorption and oxygen tension in the rat kidney, was studied. Adrenaline inhibited sodium excretion decreasing its filtration in glomeruli and stimulating its reabsorption in tubules. The oxygen tension in ... | 186,334 |
Intracellular enzymes of collagen biosynthesis in rat kidney in streptozotocin diabetes. | The activities of the four enzymes catalyzing intracellular post-translational modifications of the collagen polypeptide chains were assayed in the kidneys of rats with streptozotocin diabetes. When the changes in the four enzyme activities were expressed per milligram of protein in the 15,000 X g supernatant of the ki... | 186,346 |
Effect of propranolol on bile acid- and cholera enterotoxin-stimulated cAMP and secretion in rabbit intestine. | Stimulation of net secretion by deoxycholic acid (DCA) in the colon and by cholera enterotoxin (CE) in the jejunum is mediated by cAMP. Propranolol (Pr) inhibits adenylate cyclase (AC) activity and net secretion induced by bile acid in the colon. The aim of this study was to assess the organ specificity of DCA and CE a... | 186,351 |
13C-NMR studies of the membrane structure of enveloped virions (vesicular stomatitis virus). | The mobility of the lipids in the bilayer of the envelope of vesicular stomatitis virus has been probed over its complete space by the biosynthetic incorporation of [N-13CH3]- choline as a probe for the polar head groups and [3-13C]- and [11-13C] oleic acid and [16-13C]- palmitic acid for the hydrophobic region of the ... | 186,376 |
The co-carcinogenic activity of 4-nitropyridine-1-oxide (4-NPO) and prevention of transformation by type-specific anti-viral antibodies. | Fischer rat embryo cells chronically infected with Rauscher murine leukemia virus, and known to be sensitive to transformation by potent chemical carcinogens, were transformed by the weak carcinogen 4-nitropyridine-1-oxide. Transformed cells grew in semi-solid agar and produced tumors in newborn Fischer rats. Transform... | 186,397 |
Immunological monitoring and immunotherapy in carcinoma of the lung. | One hundred and seven patients with carcinoma of the lung underwent immunologic testing, and 62 of these patients were randomized to an immunotherapy protocol comparing the effects of Pasteur strain BCG, either alone or combined with allogeneic tumor cells, to the effects of no immunotherapy. Patients with residual dis... | 186,417 |
Expression of viral proteins in mammalian cells transformed by avian sarcoma viruses. | The expression of viral proteins in nine lines of hamster and rat cells transformed by avian sarcoma viruses (ASV) was studied by indirect immunofluorescence with monospecific antisera to purified gp85 and p27 of AMV-B and a polyvalent antiserum to all the p proteins of this same virus. The lines of ASV-transformed cel... | 186,419 |
Differences in adenylate cyclase activities in murine normal cells and bladder tumor cells in tissue culture. | Cyclic AMP may be involved in the modulation of cell growth. The present work sought to further define differences between normal cells and tumor cells in their cyclic AMP system. Mouse embryo fibroblasts and murine bladder transitional epithelium tumor cells were grown in vitro; at various times, adenyl cyclase activi... | 186,432 |
Plaque dissociation of herpes simplex viruses: biochemical and biological characters of the viral variants. | Polycaryocytogenic (P) and non-polycaryocytogenic, or aggregating (A), stable variants were selected from a herpes simplex virus type 1 (HSV-1) and from a herpes simplex virus type 2 (HSV 2) which had not been deliberately exposed to known mutagenic agents. The P variant of HSV-1 (FP) differed from the A variant (FA) i... | 186,433 |
Serologic comparison of hepatitis A antigen to echovirus 25 isolate CR69(076) recovered from a patient with viral hepatitis. | A serologic comparison was performed by immune electron microscopy between hepatitis A antigen (related to the MS-1 strain of hepatitis A virus) and a viral isolate, CR69(076), from the stool of a patient in Costa Rica with viral hepatitis. CR69(076) was not antigenically related to hepatitis A antigen but was related ... | 186,440 |
High-speed liquid and thin-layer chromatography of polychlorinated biphenyls. | High-speed liquid chromatography in the system silica gel/dry n-hexane and ultraviolet spectrometry have been used to study the composition of various types of commercially available mixtures of chlorinated biphenyls. Special attention has been paid to the analysis of highly chlorinated products. In addition to data pr... | 186,470 |
Unconjugated dehydroepiandrosterone plasma levels in normal subjects from birth to adolescence in human: the use of a sensitive radioimmunoassay. | A specific and sensitive radioimmunoassay for measuring unconjugated plasma dehydroepiandrosterone (DHA) has been developed and the results expressed in ng/100 ml. Mean values +/-1 SD were in mixed cord blood 593.3 +/- 186.5 in 21 females and 712.7 +/- 190.9 in 18 males. During the first day of life the peripheral plas... | 186,482 |
Insulin release and cyclic AMP accumulation in response to glucose in pancreatic islets of fed and starved rats. | The dose as well as the time kinetics of insulin and adenosine-3', 5' -monophosphate (cyclic AMP) responses to glucose were compared in pancreatic islets of fed and starved rats. There was a preferential impairment of the early phase of glucose-induced insulin release in perifused islets of rats starved for 16 and 48 h... | 186,486 |
The effects of colestipol on the metabolism of very-low-density lipoproteins in man. | Bile acid-binding resins, currently used for the treatment of Type II hyperlipoproteinemia, decrease LDL cholesterol by 25 to 35 per cent. Elevations of plasma triglycerides sometimes occur with resin therapy, suggesting that alterations in metabolism may not be confined solely to LDL. To characterize the effects of th... | 186,544 |
Longevity syndromes: familial hypobeta and familial hyperalpha lipoproteinemia. | Longevity and morbidity and death from myocardial infarction were examined in eight kindreds with familial hypobeta lipoproteinemia and in 18 kindreds with familial hyperalpha lipoproteinemia. Expectation of life for males and females from kindreds with hypobeta lipoproteinemia was 9 and 12 years longer (p less than or... | 186,545 |
Effect of epinephrine on the oxidative desaturation of fatty acids in the rat. | The effect of epinephrine on the oxidative desaturation of fatty acids by liver microsomal preparations of rats has been studied. Administration of epinephrine (1 mg/kg body weight) produced a significant decrease in desaturation of [l-14C]=linoleic acid to gamma-linolenic acid and of [L-14C]alpha-linolenic acid to act... | 186,549 |
Accumulation of 99mTc-diphosphonate in four patients with hepatic neoplasm: case reports. | The accumulation of bone-seeking radiopharmaceuticals in extraosseous lesions has been reported in patients with myocardial infarctions, cerebral infarctions, and some soft-tissue tumors. While the precise mechanisms involved remain uncertain, the spectrum of abnormalities exhibiting such accumulation increases. In our... | 186,568 |
Immunization of rabbits with secific components of postsynaptic membrane. Acetylcholinesterase and cholinergic receptor. | Rabbits were immunized versus either an acetylcholinesterase- or a cholinergic receptor-rich fraction isolated from the electric organ of Torpedo marmorata. In both groups of animals we obtained a production of specific antibodies detected by immunodiffusion without cross reaction for the two antigens. Only rabbits imm... | 186,567 |
Effect of antibiotics on growth of the immature rat. | The purpose of this study was to quantify the growth promoting effect of a mixture of antibiotics for rats eating diets deficient in protein or an essential amino acid. Male albino weanling rats (70 to 80 g weight, 4 weeks old) were fed (a) a control diet containing all other required nutrients and varying amounts of c... | 186,572 |
[Post-operative radiotherapy of hemisphere gliomas in the adult. A statistical study of 134 cases (author's transl)]. | A series of 134 adults patient with hemisphere gliomas who received post-operative radiation after surgical excision, either by conventional radiotherapy or high energy radiation, between 1966 and 1972 in the Léon-Bérard Centre. Overall survival for grade I and II astrocytomas at two years was 15/29, or 51%. Overall su... | 186,600 |
Evaluation of BCG administered by scarification for immunotherapy of metastatic hepatocarcinoma in the guinea pig. | In inbred guinea pigs, administration of Mycobacterium bovis strain BCG by scarification at a site distant from an excised skin tumor, but in the regional lymph node drainage, was evaluated for its immunotherapeutic effect on the development of lymph node metastases. Scarification was performed after surgical excision ... | 186,611 |
Oncornavirus-like particles in baboon type C virus-infected chimpanzee lung cells (SFRE:CL-1). | Intracytoplasmic type A particles were observed in a fetal chimpanzee lung culture (SFRE:CL-1) inoculated with type C virus-containing supernatants from a coculture of baboon placenta and SFRE:CL-1 cells. Budding, immature, and mature type C particles were also noted. In thin section, spike-like structures were rarely ... | 186,614 |
Chronic Herpesvirus saimiri infection in an owl monkey. | An adult owl monkey (Aotus tricirgatus) used for immunologic studies of Herpesvirus saimiri (HVS) developed early, late, membrane, and neutralizing antibodies to HVS approximately 3 weeks after the beginning of the experiment. HVS was isolated by the cocultivation of peripheral blood for over 1 year. No clinical, gross... | 186,615 |
Feline oncornavirus-associated cell membrane antigen. VI. Cytotoxic antibody in cats exposed to feline leukemia virus. | Feline leukemia virus (FeLV)-infected specific pathogen-free (SPF) cats, normal uninfected SPF cats, and healthy cats from leukemic households were tested for antibody reactive to the feline oncornavirus-associated cell membrane antigen (FOCMA)-containing target cell line FL-74 by microcytotoxicity and indirect membran... | 186,623 |
Transplantation of human tumors in nude mice. | Ninety-one human tumors, including various common carcinomas, low-grade malignant tumors, and benign tumors, were transplanted into athymic nude mice. Tumor take was confirmed histologically for 22 neoplasms at the initial transplantation, and 14 serially transplantable tumors were established, including some hitherto ... | 186,624 |
Formation of larger cell aggregates by transformed cells: an in vitro index of cell transformation. | Virus-transformed rat and hamster embryonic cells formed larger cell aggregates than those formed by normal counterpart cells within 1-3 days. This aggregate property correlated with growth in soft agar and tumorigenicity. | 186,626 |
Proteolytic enzyme activity in the granulation tissue of the human burn wound. | A procedure for adsorbing enzymes from the human burn wound onto solid sheets of substrate is described. Using this technique, low levels of enzyme activity with chymotrypsin-like specificity can be demonstrated in the wound approximately 2 weeks after injury. This activity disappears at about 5 weeks after the burn. T... | 186,629 |
RNA synthesis in cells infected with herpes simple virus. XIII. Differences in the methylation patterns of viral RNA during the reproductive cycle. | Herpex simplex virus 1 (HSV-1) RNA labeled with with [methyl-3H] methionine at various times during the infectious cycle and purified by hybridization to viral DNA was analyzed for the presence of methylated nucleotides. The data indicate the following. (i) RNA labeled from 0 to 14 h postinfection and accumulating in t... | 186,635 |
Genetic evidence for a product of the Fv-1 locus that transfers resistance to mouse leukemia viruses. | Extracts of mouse cells have been shown to transfer to N- or B-trophic host range types of mouse leukemia viruses. The genetic specificity of the inhibition was tested in two ways: (i) by correlating the Fv-1 genotype of a number of mouse strains with the restriction-transferring activity of extracts of the respective ... | 186,636 |
Immune response against hamster erythrocytes in the low-responder mouse strains. XI. Strain difference in the effects of various microbial adjuvants. | Enhancing and suppressing effects of microbial adjuvants were studied in female mice of the C3H/He, AKR and SL strains. Propionibacterium acnes, Bordetella pertussis, BCG and yeast cell wall (YCW) were chosen as adjuvants. As antigens, we chose hamster erythrocytes (HRBC) which proved to be a weak antigen for mice. Adj... | 186,656 |
Persistent high numbers of Clostridium perfringens in the intestines of Japanese aged adults. | TSN agar was applicable for enumeration of Clostridium perfringens in fecal samples of adults but not in those of infants. It was demonstrated using TSN agar that some healthy aged adults had persistently carried C. perfringens at levels ranging from 10(7) to 10(9), while some others ranged from 10(3) to 10(6) per ml v... | 186,657 |
Treatment with gentamicin monitored by serum antibiotic assay. | Gentamicin is very widely accepted as the most effective antibiotic for treatment of a number of serious Gram-negative infections, but one disadvantage associated with its use is the risk of toxicity particularly affecting the eighth nerve. An account is given of eight years' experience with this antibiotic in which ob... | 186,696 |
Relationship between the level of cAMP and the contractile force under stimulation of alpha- and beta-adrenoceptors by phenylephrine in the isolated rabbit papillary muscle. | The time course of changes of the level of 3',5'-cyclic AMP (cAMP) and of the tension developed under stimulation of alpha- and beta-adrenoceptors by phenylephrine was investigated in the isolated rabbit papillary muscle. Furthermore the dose-response relationships for increases of cAMP and of developed tension elicite... | 186,721 |
Binding of botulinum neurotoxin to the synaptosome fraction of rat brain. | The radioactive 125I-labelled neurotoxin of C. botulinum type A, when incubated with rat brain homogenate, is bound selectively to the synaptosome fraction. Intact toxin was liberated from the synaptosome fraction by treatment with Triton X-100, SDS, trypsin or neuraminidase. | 186,722 |
The effect of administered corticosteroids on the growth of children. | The growth-inhibiting effect of exogenous corticosteroids has been reported in many papers. Most of them have concerned the clinical problems of asthma and rheumatoid arthritis but it is probable that the underlying disease is relatively unimportant in determining the effect on growth. Steroid therapy on alternate days... | 186,769 |
Relationship of gangliosides to the structure and function of thyrotropin receptors: their absence on plasma membranes of a thyroid tumor defective in thyrotropin receptor activity. | Plasma membranes derived from rat thyroid tumor (1-8R) which is unresponsive to thyrotropin but is responsive to dibutyryl adenosine 3':5'-cyclic monophosphate bind less than 20% of the [125I] thyrotropin which can be bound to plasma membranes from normal rat thyroids under conditions which optimize tumor membrane bind... | 186,783 |
Butanediols: selection, open field activity, and NAD reduction by liver extracts in inbred mouse strains. | Mice from the high-ethanol preferring C57BL strain and low-ethanol preferring DBA strain were tested for their preference for butanediols. The C57BL strain showed a significantly higher preference for a 10% (v/v) solution of 1,3-butanediol than the DBA strain. The C57BL strain also showed a significantly greater consum... | 186,819 |
Effect of thyrotropin-releasing hormone (TRH) and antidepressant agents on brain stem and hypothalamic multiple unit activity in the cat. | The EEG and MUA (multiple unit activity) of mesencephalic reticular formation (MRF), area hypothalami posterior (PH), and area hypothalami anterior (AH) were studied in chronically implanted freely moving cats. The effects of thyrotropin-releasing hormone (TRH) and some antidepressant agents were tested on neuronal act... | 186,834 |
Routes of calcium movement across the chick chorioallantois. | The mechanism of calcium transport across the chick chorioallantois has been studied in vivo. The 15 day old membrane contains no calcium binding protein (CaBP) and attempts to stimulate transport by adding this protein or vitamin D metabolites did not enhance the normal transport process. The mitochondrial and microso... | 186,837 |
Depression of REM sleep in cats by nisoxetine, a potential antidepressant drug. | Nisoxetine, a potent and specific inhibitor of norepinephrine uptake, suppressed REM sleep in cats. Oral doses as small as 0.1 mg/kg were effective during the first 2 1/2 hours of a recording session; 0.25 mg/kg, for 5 hours. The amount of slow wave sleep increased in cats that received 0.1-1.0 mg/kg of nisoxetine. The... | 186,850 |
[Femoral nerve paralysis complicating anticoagulant treatments. A propos of 3 cases]. | The authors report 3 new cases of femoral nerve paralysis complicating anticoagulant treatment. The first sign was pain, the neurological signs occurred later and the patient usually recovered but recovery was sometimes incomplete. The pathogenesis is not clear : a muscle hematoma, ischemia of the nerve trunk, and intr... | 186,873 |
[Practical value of gastric intubation during peptic ulcer. Analysis of 410 cases, after Histalog stimulation]. | This study was carried out in 410 subjects, collecting gastric fluid for one hour, after 15 minutes emptying and one hour, after 15 minutes emptying and one hour's study of basal secretion using, as stimulant, Histalog at a maximal dose of 2 mg/kg without exceeding 130 mg. The object of this statistical analysis was to... | 186,880 |
[New data on the disease induced by laxatives]. | The authors report 5 personal case and recall the physiopathological mechanisms of metabolic disorders observed during the abuse of laxatives. They emphasize the fact that electrolyte losses were not only fecal, but also aggravated by frequent use of diuretics or dietary restriction. One may oppose the constant, very m... | 186,881 |
[Sacrocoxitis due to common bacteria]. | The authors report 11 cases of sacro-coxitis due to pyogenic bacteria observed over a period of seven years and emphasize that this is a disease of young women recently in labour, usually due to staphylococci which enter through the genital tract. The other methods of contamination are urinary, cutaneous and dental. Th... | 186,887 |
[Hand, foot and mouth disease]. | Two characteristic cases encountered in young adults led the authors to present the hand foot and mouth syndrome. They report the characteristic distribution and vesicular appearance of the lesions. The course was benign. The viral origin of the disease was more or less easily confirmed by cell culture, inoculation in ... | 186,899 |
[Hepatic tissue respiration in pathology of the biliary system and the effect of hyperbaric oxygenation on the course of hepatic insufficiency]. | Under study was the effect of hyperbaric oxygenation in 46 patients on the course of bile tract diseases, complicated with hepatic insufficiency. Hyperbaric oxygenation was found to render a positive effect on the course of hepatic insufficiency. | 186,926 |
[Virological investigations in peripheral idiopathic facial pareses (author's transl)]. | Virological investigations were carried out on the serum of 62 patients suffering from peripheral facial pareses. The aetiology was definitely attributable to a virus infection in 12 of these cases. Neuropathological changes induced by these neurotropic viruses, predisposing anatomical features and pathogenesis are dis... | 186,960 |
Functional and ultrastructural studies of the effects of human interferon on cell membranes of in vitro cultured cells. | The effect of human leukocyte interferon on cultured U-amnion cells was examined, and several biological parameters were registered. Multiplication of Vesicular stomatitis virus and the virus-produced cytopathogenic effect was prevented. The growth rate of uninfected cells was reduced, as well as the spontaneous releas... | 187,008 |
Responses of the denervated rat urinary bladder to alfa adrenoceptor stimulation. | The contractile responses of the rat detrusor muscle to noradrenaline were increased 2-3 weeks after partial denervation but had returned to control values 6-9 weeks after the denervation, probably because of reinnervation of denervated muscle cells. 6-9 weeks after partial denervation the excitatory alpha-adrenoceptor... | 187,014 |
Stimulation by TSH of prostaglandin synthesis in pig thyroid. | The liberation of arachindonate in the thyroid occurs at the expense of two distinct pools of precursors. (1) the phosphatidylinositol through a process Ca2+-dependent and cyclic AMP-independent; and (2) the triglycerides by a cyclic AMP-dependent lipase, in which the involvement of cyclic AMP-dependent protein kinase ... | 187,042 |
Advances in vitamin D metabolism as they pertain to chronic renal disease. | Many clinical similarities between renal osteodystrophy and nutritional rickets have suggested that a defect in either the metabolism or action of vitamin D exists in chronic renal failure. The discovery of the kidney as the organ that manufactures the active metabolite of vitamin D has provided direct evidence for a r... | 187,055 |
Impaired microtubule function correctable by cyclic GMP and cholinergic agonists in the Chediak-Higashi syndrome. | The Chediak-Higashi (CH) syndrome of man and several animal species is characterized by the presence of abnormal giant granules in all granule-containing cells and by defects in chemotaxis and lysosomal degranulation during phagocytosis in polymorphonuclear leukocytes (PMNs). Since similar functional abnormalities have... | 187,062 |
Effectiveness of community services for discharged mental hospital patients. | This study of 579 state hospital patients charts the pattern of their care in the community in the two or three years following hospital discharge, and examines the relationship of aftercare services to readmission rates. Findings suggest that, among the most chronic patients, a substantial number of aftercare visits m... | 187,064 |
Cryoultramicroscopy of lower vertebrate retina. | Cryoultramicroscopy was carried out in the retina of lower vertebrates. Myeloid bodies of the pigment epithelium of turtles and synaptic vesicles and ribbons of frogs were stained negatively. Numerous altered, shaped, dense bodies, containing granular and myelinlike matrices, were found in the retinal pigment epitheliu... | 187,072 |
Effects of metyrapone and ACTH on intestinal absorption of immunoreactive bovine IgG in cesarean-derived pigs. | Newborn cesarean-derived pigs were injected with 5.0 mg of metyrapone/kg, or 1 USP U of ACTH/kg, or the vehicle 1.0 ml of 44 mM sodium tartrate and 88 mM NaCl soon after delivery (0 hour) and again 4, 8, and 12 hours later. Beginning at 2 hours, each pig in the 3 groups was given 40 ml of pooled bovine colostrum/kg by ... | 187,090 |
Influence of adenosine, cAMP and db-cAMP on responses of the isolated terminal guinea-pig ileum to electrical stimulation. | Adenosine, cAMP and db-cAMP were found to inhibit nerve-mediated contractions of the terminal ileum elicited by electrical stimulation (ES). Responses to ES at 30 Hz, which are believed to be of adrenergic origin, were considerably more affected than the responses to ES at 3 Hz, which may be of purinergic orign. The fo... | 187,132 |
Electron spin resonance study of changes during development of solid yoshida tumour. II. Paramagnetic metal ions. | Electron spin resonance (ESR) spectroscopy was used to examine changes in the concentration of paramagnetic metal ions in Yoshida tumours carried by female Wistar rats. Blood, spleen and lymph nodes from these animals were also examined by ESR. A decrease in the concentration of a paramagnetic species associated with m... | 187,210 |
Metabolism of pyridoxine in the liver of vitamin B-6-deficient rats. | The metabolism of [6-3H]pyridoxine - HCl was investigated in the liver of vitamin B-6-deficient rats. Rats were made vitamin B-6 deficient by feeding ad libitum for 42 days a diet lacking pyridoxine but otherwise optimal. Animals were each injected intraperitoneally with 33 muCi of [6-3H] pyridoxine - HCl and killed at... | 187,222 |
Phospholipid metabolism and lysosomal enzyme secretion by leukocytes. Effects of dibutyryl cyclic adenosine 3':5'-monophosphate and ATP. | The effects of dibutyryl cyclic adenosine 3':5'-monophosphate and ATP on isotope incorporation into phospholipids and the release of beta-glucuronidase into the extracellular medium were studied in polymorphonuclear leukocytes from guinea pig peritoneal exudates. Exogenous dibutyryl cyclic adenosine 3':5'-monophosphate... | 187,223 |
The development of phosphomonesterases in the testes of prepuberal chicks. | 1. The biochemical development and histochemical localisation of phosphomonoesterases in the testes of prepuberal chicks have been studied. 2. Maximum acid phosphatase activity was observed at 12 weeks with a decrease in enzyme activity after this age, whereas alkaline phosphatase activity fluctuated with age. 3. Acid ... | 187,228 |
In vitro synthesis of simian virus 40 DNA. II. Evidence for a repair mechanism. | The technique of density labeling of DNA by BrdU was used to characterize the material synthesized in vitro by cytoplasmic extracts of SV40 infected cells incubated in the presence of simian virus 40 (SV40) DNA component I molecules (Girard et al, Biochimie, this volume). In a first experiment, the template was labeled... | 187,251 |
Probenecid-induced accumulation of cyclic nucleotides, 5-hydroxyindoleacetic acid, and homovanillic acid in cisternal spinal fluid of genetically nervous dogs. | These studies have been conducted on 40 dogs, twenty each of a genetically nervous strain and of a normal strain of short-haired pointers. The nervous strain after about age 3 months displays extreme hypervigilance, timidity, human avoidance, and often shows catatonic-like muscle rigidity when in the presence of humans... | 187,258 |
Thymidine kinase gene transfer by herpes simplex virus. | The current state of knowledge concerning the biochemical transformation by Herpes Simplex virus (HSV) of mammalian cells lacking the enzyme thymidine kinase (TK) is reviewed. Transformation of thymidine kinase negative mouse cells (LTK-) to the TK+ phenotype by ultraviolet light-inactivated HSV preparations depends bo... | 187,268 |
A perfusion culture system for virus vaccine manufacture in diploid cell cultures. | Development of a new perfusion culture system for the production of attenuated poliomyelitis virus in cultures of diploid cells is described. The growth characteristics of the diploid cells (MRC-5) were found to be normal in the perfused system. Procedures for the routine production of cell cultures at twice the cell d... | 187,274 |
Histomorphometric determination of formation rates of archaeological bone. | Archaeological rib samples were subjected to quantitative histologic analysis to determine rates of cortical bone formation. Histologic features are usually well enough preserved to permit the determination of mean annual Haversian bone formation rate averaged over the life span of the individual. Moreover, gross estim... | 187,286 |
Responses of fetal rat bone cells and bone organ cultures to the ionophore, A23187. | The ionophore A23187 produced a rapid transient increase in the rate of calcium uptake by isolated fetal rat bone cells. There was no effect on calcium efflux or total cellular calcium. The magnitude of the effect on influx was amplified when the cell were incubated at 4 degrees C. Cellular metabolic functions and reso... | 187,287 |
Deficiency of S-adenosylmethionine-homocysteine methyltransferase activity in hepatoma cells. | S-Adenosylmethionine-homocysteine methyltransferase, which catalyzes synthesis of methionine from homocysteine, with the use of S-adenosylmethionine as the methyl donor, is absent in tumor tissue such as rat ascites hepatoma and Morris hepatoma but is present in rat liver homogenate. Absence of the enzymatic activity i... | 187,333 |
Modifications of drug metabolism by disulfiram and diethyldithiocarbamate. II. D-Glucuronic acid pathway. | Hepatic enzymes connected with the formation and metabolism of free D-glucuronic acid were affected in rats after treatment with disulfiram or diethyldithiocarbamate (300 mg/kg, intragastrically, per day, 4 X). The activities of UDPglucose dehydrogenase, UDPglucuronic acid pyrophosphatase, UDPglucuronosyltransferase an... | 187,353 |
Is NIMH's dream coming true? Wyoming centers reduce state hospital admissions. | Do comprehensive community mental health services reduce admissions to state hospital? A recent Nader report says no. Surprisingly few studies have documented the success of comprehensive community mental health programs. Wyoming had four unique characteristics that aided in evaluating the impact of centers on hospital... | 187,372 |
Electron microscopy of the cotton fibre: new observations on cell wall formation. | The ultrastructure of the cotton fibres was examined after developing successful fixation methods. Fibre cells were fixed at different stages of development. In cells which were elongating and producing primary cell walls, the Golgi apparatus appeared to be directly involved in secretion and synthesis of primary wall c... | 187,389 |
Regulation of lactogenic hormone binding in rat liver by steroid hormones. | We have measured the effects of testosterone propionate, medroxy-progesterone acetate and cortisol on the binding of ovine [125I]iodoprolactin to 100,000 X g particulate fractions from liver of normal and estrogen-treated female rats. In untreated animals 6.7 +/- 1.1% (SD) of the radioactivity added to 0.5 mg of membra... | 187,403 |
Evidence for the existence of a histamine H2-receptor in the mouse thyroid. | The existence of a histamine H2-receptor in the thyroid was investigated. Histamine in vitro stimulated the formation of cyclic AMP and colloid droplet formation in mouse thyroid lobes. Stimulation by histamine of cyclic AMP formation in mouse thyroid lobes was significantly inhibited by metiamide, a histamine H2-recep... | 187,408 |
Specific and nonspecific multiple unit activities during the onset of pentylenetetrazol seizures. II. Acute lesions interrupting nonspecific system connections. | Nonspecific cortical, thalamic, mesencephalic, and pontine multiple unit activities (MUA) and changes in EEG and MUS of the sciatic nerve after threshold pentylenetetrazol activation were studied in three groups of animals in which neuronal connections were interrupted at three different levels of the central nervous s... | 187,424 |
Effects of trypsin and phospholipases A2 and C on enzyme organization in testis microsomes. | Pregnenolone and progesterone concentrated in the microsomal fraction of cryptorchid mouse testis compared with mitochondria and cytosol. While the concentrating mechanisms had high capacity and low association constants the effect did not seem to be due to nonspecific solubility in the lipid components since 17-hydrox... | 187,506 |
The standardization of Cl. perfringens antigens and antisera. | The preparation of laboratory standard antitoxins against Cl. perfringens beta and epsilon toxins is described. These antitoxins are suitable for the quantitative determination of the corresponding antigens by means of the flocculation test. The flocculation test was, however, shown to be more suitable for determining ... | 187,510 |
[Standardization of the control technics of Clostridium perfringens vaccine. Use of a reference vaccine]. | Control of Cl perfringens toxins. It is possible to employ a standardized technique for the control of the toxins of Cl. perfringens. Titres of LD50 per ml for the mouse and L+/50 per ml may be expressed with a confidence limit of 1.5. The assay of toxins by L+/50 (relating to their antigenic value) permits the establi... | 187,511 |
Lacalization of the SV40 T antigen in hamster cells transformed by PARA(3ct)-adenovirus 7. | The localization of the SV40 T antigen in hamster cells transformed by the PARA(3ct)-adenovirus 7 variant and, for comparison, by SV40 was examined. The H50 cell strain (transformed by SV40) and the P7-/BL/SV/OD strain (transformed by PARA(3ct)-adenovirus 7) showed a preferential localization of the SV40 T antigen in t... | 187,551 |
Comparison of DNA polymerase activities induced by herpes simplex virus types 1 and 2. | HSV-2-induced a salt-stimulated DNA polymerase activity in HEp-2 cells at similar levels to that induced by HSV-1 virus at multiplicites of infection from 2 to 20 PFU/cell. De novo synthesis of protein and mRNA was required for DNA polymerase induction by both types. DNA polymerase activity of three strains of each typ... | 187,552 |
Studies on the turnover rates of ornithine aminotransferase in Morris hepatoma 44 and host liver. | The ornithine aminotransferase [EC 2.6.1.13] content of Morris hepatoma 44 is about 15 times higher than that in normal liver. The turnover rates of ornithine amino-transferase in hepatoma 44 and host liver were determined using L-[14C]leucine. Studies on the incorporation of radioactive leucine into ornithine aminotra... | 187,580 |
Steady state and exchange kinetics of pyruvate, phosphate dikinase from Propionibacterium shermanii. | Evidence is presented based on requirements for exchange in the partial reactions, initial velocity and exchange kinetics and product inhibition, that the pyruvate, phosphate dikinase reaction of propionibacteria occurs by a nonclassical Tri Uni Uni Ping Pong mechanism. The mechanism involves a pyrophosphoryl enzyme, a... | 187,600 |
Intranuclear membranous inclusions in oocytes of a viviparous teleost (Xiphophorus helleri). | Intranuclear inclusions were observed in oocytes of Xiphophorus helleri during prophase I. In osmium-fixed leptotene nuclei, the inclusions were made up of groups of membrane-limited vesicles or tubules with pale contents, situated near the inner nuclear membrane with which some of them exhibited apparent continuities.... | 187,605 |
Extrinsic modulation of human T-lymphocyte E rosette function associated with prolonged hepatocellular injury after viral hepatitis. | Defective T-lymphocyte E rosette (ER) function associated with viral hepatitis A and B may be due to mechanisms extrinsic or intrinsic to the target lymphocyte. The extrinsic defect is induced by an immunoregulatory plasma lipoprotein (RIF) and has the capacity to regenerate ER function in vitro. The intrinsic defect i... | 187,620 |
Histamine modulation of eosinophil migration. | Preincubation of eosinophils with 10(-5) M or higher concentrations of histamine inhibited the eosinophil chemotactic response to endotoxin-activated serum whether by using the nucleopore filter assay and counting the cells migrating through the filter, or by using the Zigmond-Hirsch assay and counting the cells at eac... | 187,697 |
Immune response to acute otitis media in children III. Implications of viral antibody in middle ear fluid. | With the technique of radioimmunodiffusion and indirect FA staining, IgA antibody to measles, mumps, rubella, and polio-1 were determined in serum and middle ear fluid (MEF) of 103 patients with otitis media. The occurrence of IgA viral antibody in MEF and its absence in simultaneously drawn serum was used as an indica... | 187,700 |
Isolation of the matrix (membrane) protein of vesicular stomatitis virus by gel filtration in guanidine hydrochloride. | The L, N and M proteins of vesicular stomatitis virus (VSV) were resolved from each other by gel filtration in the presence of 6 m-guanidine hydrochloride. Amino acid analysis for purified M protein of VSV showed that its chemical composition differed from those of influenza and SV5 M proteins. | 187,728 |
Evidence for species-specific substrate-site-directed inactivation of rabbit adenylate kinase by N6-(6-iodoacetamido-n-hexyl)adenosine 5'-triphosphate. | Adenosine 5'-triphosphate (ATP) derivatives bearing iodoacetylamino-n-alkyl substituents [(CH2)nNHCOCH2I] on N6 were synthesized as potential ATP-site-directed irreversible inhibitors of adenylate kinases from rabbit, pig, and carp muscle. When n was 5 no enzyme was progressively inhibited (inactivated) by 1 mM inhibit... | 187,748 |
Adenosine 3',5'-cyclic monophosphate levels in x-ray-induced small-bowel adenocarcinoma in the rat. | X-ray-induced adenocarcinomas in the small bowels of outbred Lewis Brown Norway and Holtzman rats contained significantly lower concentrations of adenosine 3',5'-cyclic monophosphate than did normal rat intestinal tissue. No significant differences were observed between the intracellular concentrations of the nucleotid... | 187,764 |
Nonimmune and immune surveillance. I. Growth of tumors and normal fetal tissues grafted into newborn mice. | Growth of various fetal tissues and transplantable tumors in syngeneic newborn and adult mice [BALB/c, DBA/2, and (CBA X C57BL/6J)F1] was compared. Fetal skin, a mixture of all fetal tissues, and tumors were transplanted. The tumors arose spontaneously [hepatomas, mammary gland adenocarcinoma (MGAC)] or resulted from m... | 187,765 |
Cylindroid lamella-particle complexes in lymphoma cells by northern pike (Esox lucius). | Cells of the malignant lymphoma of northern pike of Canada contained cylindrical structures with central cores of cytoplasmic elements and walls composed of lamellae in a spiral arrangement, separated by ribosome-like particles or by membranes. These cylindroids were similar, if not identical, to structures in the cell... | 187,772 |
Sequential hepatic histologic and histochemical changes produced by diethylnitrosamine in the rhesus monkey. | Six young adult male rhesus monkeys were given diethylnitrosamine ip for 3-5 years. Liver biospies were done monthly. After 6 months, biopsy specimens showed individual hepatocytes and small foci of hepatocytes that were intensely positive for glycogen. During the second and later years, larger foci of such cells devel... | 187,798 |
Comparative studies on isolated rat hepatocytes and AS-30D hepatoma cells with leucocidin from Pseudomonas aeruginosa. | Leucocidin, one of the cytotoxic principles of Pseudomonas aeruginosa induces potassium loss and swelling in isolated hepatocytes and in AS-30D ascites hepatoma cells in a dose and time dependent manner. Hepatoma cells are more sensitive than normal hepatocytes. As shown by scanning electron microscopy the volume incre... | 187,964 |
Infection of human embryo cells with avian sarcoma virus B77 in vitro. | Human embryo cells were infected with avian sarcoma virus B77--Hu(B77). The virus genome was detected in the Hu(B77) cells during subsequent cell passages in the uncloned cells as well as in single-cell clones. Despite the presence of the virus genome in cells, the growth properties did not differ from uninfected cells... | 187,972 |
Light microscopy of selected viral diseases (morphology of viral inclusion bodies). | A significant number of viruses produce intracellular changes, inclusion bodies, which do have specific and consistent features important to their identification. The inclusions must fulfill the designated criteria for the specific virus or a member of that group. The specific features of viral inclusions are consisten... | 188,006 |
Hydrolysis of nicotinamide adenine dinucleotide by choleragen and its A protomer: possible role in the activation of adenylate cyclase. | Choleragen and the isolated A protomer catalyzed the hydrolysis of NAD to ADP-ribose and nicotinamide. The protein with NADase activity (NAD nucleosidase; NAD glycohydrolase, EC 3-2-2-5) migrated on polyacrylamide gels with choleragen, and chromatographed on Bio-Gel P-60 columns with the A protomer. The NADase activity... | 188,038 |
Dysfibrinogenaemia and primary hepato-cellular carcinoma. | The mechanisms of blood coagulation and fibrinolysis have been evaluated in 28 black adult Africans with primary hepato-cellular carcinoma (HCC). A characteristic pattern of abnormalities has been defined. Dysfibrinogenaemia appears to be a useful biological marker for the disease. The reptilase test (RT) is a simple, ... | 188,064 |
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