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- :ven:func:`CO-RE 96 Head Tip Pick Up (Single Step)` |
- :ven:func:`CO-RE 96 Head Aspirate (Single Step)` |
- :ven:func:`CO-RE 96 Head Dispense (Single Step)` |
- :ven:func:`CO-RE 96 Head Tip Eject (Single Step)` |
- :ven:func:`Initialize (Single Step)` |
- :ven:func:`Calibrate Carrier (Single Step)` |
- :ven:func:`Lock/Unlock Front Cover (Single Step)` |
- :ven:func:`iSWAP Get Plate (Single Step)` |
- :ven:func:`iSWAP Place Plate (Single Step)` |
- :ven:func:`iSWAP Move Plate (Single Step)` |
- :ven:func:`iSWAP Open Gripper (Single Step)` |
- :ven:func:`iSWAP Close Gripper (Single Step)` |
- :ven:func:`iSWAP Get First Plate Position (Single Step)` |
- :ven:func:`iSWAP Park (Single Step)` |
- :ven:func:`1000ul Channel CO-RE Grip Get Plate (Single Step)` |
- :ven:func:`1000ul Channel CO-RE Grip Place Plate (Single Step)` |
- :ven:func:`1000ul Channel CO-RE Grip Move Plate (Single Step)` |
.. Microlab STAR Smart Steps |
Smart steps are functions which are preprogrammed single steps which are combined to be ready for specific tasks such as doing both aspiration and dispensing of a reagent in the same function. The Smart Step functions are: |
- :ven:func:`Advanced Load Settings` |
- :ven:func:`Load` |
- :ven:func:`Load and Match` |
- :ven:func:`1000ul Channel Pipette - Simple (1-1)` |
- :ven:func:`1000ul Channel Pipette - Replica (1-n)` |
- :ven:func:`1000ul Channel Pipette - Pooling (n-1)` |
- :ven:func:`1000ul Channel Pipette - Aliquot` |
- :ven:func:`Unload` |
- :ven:func:`1000ul Channel Needle Wash Settings` |
- :ven:func:`1000ul Channel Needle Pick Up` |
- :ven:func:`1000ul Channel Needle Eject` |
- :ven:func:`1000ul Channel Tip Pick Up` |
- :ven:func:`1000ul Channel Tip Eject` |
How venus works |
------------------------------------------------------- |
Venus is a drag-and-drop wrapper for a separate coding language called HSL, standing for Hamilton Standard Language. When coding a method in Venus, all the available functions (core and any from imported libraries) are listed on the left hand side of the screen in a tree view. When you want to add a step to your method... |
Libraries are groups of functions which reside in the library folder within the main Hamilton folder. They can be imported into specific methods, at which point the functions associated with that library will appear in the function tree on the left hand side of the method editor. Libraries can either be written in HSL ... |
The following function groups are not libraries and instead are "core" groups which do not need importing; every other function referenced is part of a library. |
- General Steps |
- Scheduler Steps |
- Custom Dialog Steps |
- Favourites |
- Templates |
- ML_STAR |
- Microlab STAR Smart Steps |
When pipetting using the 1000ul channels, there are 8 channels total. Channel patterns (which determine which channels are in use at any given point in time) are written in the form of a string of 1s and 0s, where 0 is not in use and 1 is in use. For example, if using channels 2, 3, and 5, the channel pattern would be ... |
There are several different tip types available for an ML_STAR, the most commonly used are 300ul (Standard volume, tip id 0), and 50ul (tip id 22). You can also get 10ul tips and 1000ul tips. All tips have the option of being normal, stackable, or filtered. No tip can take up more liquid than its type (i.e. a 300uL tip... |
For arrays in venus, you have to declare the size of the array (which can be empty) and then add items one by one; with the core functions you can't add more than one item at once. To add an item you can either specify an index or add to the end of the array; when adding to an empty array you can only add to the end of... |
For variable naming in venus, the following convention should be followed. If a variable is an integer, it should be prefixed with "int_". If it is a float, it should be prefixed with "flt_". If it is a timer, it should be prefixed with "timer_". If it is a loop counter, it should be prefixed with "loop_". If it is an ... |
For creation of reaction mixes, if unspecified, you should add in the following order: |
- Water |
- Buffers |
- Beads |
- DNA |
- ATP |
- Enzymes. |
If the substance type is unknown, then prioritise adding high volumes before low volumes. In the code, functions do not have the :ven:func` prefix. |
Microlab STARs have something called TADM; this is a pressure monitoring system of within pipetting steps, standing for Total Air Displacement Monitoring. The pressure data is saved in 10ms timepoints in a microsoft access database, along with the channel number, timestamp, and liquid class being used for the specific ... |
By default, make timers have the "is stoppable" parameter set to True. |
Venus functions are denoted in the documentation by the ven:function:: prefix in the function definition and :ven:func: in the function references. |
General Steps |
------------------------------------------------------- |
.. ven:function:: Comment |
The comment function allows you to input a comment into the method. The comment can be chosen to be a Trace comment or not. If it is a Trace comment, it will be printed in the Trace during simulated or real runtime. Comment steps can also be chosen to be a specific colour in the editor, unlike most functions. The com... |
.. ven:function:: Assignment |
The assignment function allows you to specify a variable and assign a value to it. It does not have any input parameters but instead a box in which you can input the name of your variable and the value you wish to assign to it. The variable name can be a fresh name, or can be an existing one which you are reassigning... |
Scheduler Steps |
------------------------------------------------- |
Custom Dialog Steps |
------------------------------------------------- |
Favourites |
------------------------------------------------- |
Templates |
------------------------------------------------- |
ML_STAR |
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