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B.3 Responsibility B.3.1 The Testing Authority has responsibility for ensuring, when possible, that the Sample Collection Authority and/or the DCO has any information necessary to conduct a Sample Collection Session with an Athlete who is a Minor .
This includes confirming wherever necessary, the parental consent for Testing any participating Athlete who is a Minor .
B.3.2 Where Sample collection involves an Athlete who is a Minor , the Testing Authority and/or the Sample Collection Authority shall assign, at a minimum, two Sample Collection Personnel to the Sample Collection Session.
Sample Collection Personnel shall be informed, in advance, that Sample collection involves (or may involve) Athletes who are Minors .
[Comment to B.3.2: For clarity, the two Sample Collection Personnel may be two DCOs or a DCO and a BCO or a DCO and a Chaperone .
The two Sample Collection Personnel shall always be present in the Doping Control Station for Sample Collection Sessions involv ing an Athlete who is a Minor .]
B.3.3 The DCO has responsibility for Sample collection .
B.4 Requirements B.4.1 All aspects of notification and Sample collection for Athletes who are Minors shall be carried out in accordance with the standard notification and Sample collection procedures unless modifications are necessary due to the Athlete being a Minor .
B.4.2 The Sample Collection Authority and the DCO shall have the authority to make modi fications as the situation requires as long as such modifications will not compromise the integrity, identity and security of the Sample .
All such modifications shall be documented.
B.4.3 Athletes who are Minors should be notified in the presence of an Athlete representative (who is not a Minor ) and should also be accompanied by a representative throughout the entire Sample Collection Session.
[Comment to B.4.
3: It is recommended that an Athlete who is a Minor be accompanied by an Athlete representative.
Reasonable efforts should be made by the Sample Collection Personnel to encourage the Athlete who is a Minor to have an Athlete representative throughout the Sample Collection Session and to assist the Athlete in locating one.
In ISTI – Effective 1 January 2023 Page 64 of 92 situations where the Athlete is unable to locate a representative then two Sample Collection Personnel shall always accompany the Athlete until their Sample Collection Session is completed, however, if an Athlete representative is located and present with the Athlete , the second Sample Collection Personnel is not required to accompany the Athlete with the exception of when the Athlete is ready to provide a Sample in accordance with the procedures outlined in Annex B.4.5. ]
B.4.4 Should an Athlete who is a Minor decline to have a representative present during the collection of a Sample, this does not invalidate the Test but shall be clearly documented by the DCO .
Any follow up action taken by the DCO and/or Chaperone to encourage and assist the Athlete in locating a representative should also be documented.
B.4.5 The representative of the Athlete who is a Minor , if present , shall only observ e the DCO /Chaperone during the passing of the urine Sample, unless requested by the Athlete who is a Minor to observe the passing of the urine Sample directly .
The second member of the Sample Collection Personnel shall only observe the DCO /Chaperone and shall not directly observe the passing of the Sample .
B.4.6 The preferred venue for all Out-of-Competition Testing of the Athlete who is a Minor is a location where the presence of an Athlete representative (who is not a Minor ) is most likely to be available for the duration of the Sample Collection Session, e.g., a training venue.
ISTI – Effective 1 January 2023 Page 65 of 92 ANNEX C - COLLECTION OF URINE SAMPLES C.1 Objective To collect an Athlete’ s urine Sample in a manner that ensures: a) Consistency with relevant principles of internationally recogni zed standard precautions in healthcare settings so that the health and safety of the Athlete and Sample Collection Personnel are not compromised; b) The Sample meets the Suitable Specific Gravity for Analysis and the Suitable Volume of Urine for Analysis .
Failure of a Sample to meet these requirements in no way invalidates the suitability of the Sample for analysis.
The determination of a Sample’s suitability for analysis is the decision of the relevant Laboratory , in consultation with the Testing Authority for the Sample Collection Session in question.
[Comment to C.1 (b): The measurements taken in the field for Suitable Specific Gravity for Analysis and the Suitable Volume of Urine for Analysis are preliminary in nature, to assess whether the Sample meets the requirements for analysis.
It is possible there could be discrepancies between the field readings and the final Laboratory readings due to the precision of the Laboratory equipment.
The Laboratory reading will be considered final, and such discrepancies (if any) shall not constitute a basis for Athletes to seek to invalidate or otherwise challenge an Adverse Analytical Finding.]
c) the Sample has not been manipulated, substituted, contaminated or otherwise tampered with in any way; d) the Sample is clearly and accurately identified; and e) the Sample is securely sealed in a Tamper Evident kit.
C.2 Scope The collection of a urine Sample begins with ensuring the Athlete is informed of the Sample collection requirements and ends with discarding any residual urine remaining at the end of the Athlete’s Sample Collection Session.
C.3 Responsibility C.3.1 The DCO has the responsibility for ensuring that each Sample is properly collected, identified and sealed.
C.3.2 The DCO /Chaperone has the responsibility for directly witnessing the passing of the urine Sample.
C.4 Requirements C.4.1 The DCO shall ensure that the Athlete is informed of the requirements of the Sample Collection Session, including any modifications as provided for in Annex A - Modifications for Athletes with Impairments and/or in Annex B - Modifications for Athletes who are Minors .
ISTI – Effective 1 January 2023 Page 66 of 92 C.4.2 The DCO shall ensure that the Athlete is offered a choice of Sample collection vessels for collecting the Sample .
If the nature of an Athlete’ s impairment requires that they must use additional or other equipment as provided for in Annex A - Modifications for Athletes with Impairments, the DCO shall inspect that equipment to ensure that it will not affect the integrity, identity or security of the Sample.
C.4.3 When the Athlete selects a collection vessel, and for selection of all other Sample Collection Equipment that directly holds the urine Sample, the DCO will instruct the Athlete to check that all seals on the selected equipment are intact and the equipment has not been tampered with.
If the Athlete is not satisfied with the selected equipment, they may select another.
If the Athlete is not satisfied with any of the equipment available for selection, this sh all be recorded by the DCO .
If the DCO does not agree with the Athlete that all of the equipment available for the selection is unsatisfactory, the DCO shall instruct the Athlete to proceed with the Sample Collection Session.
If the DCO agrees with the Athlete that all of the equipment available for the selection is unsatisfactory, the DCO shall terminate the urine Sample collection , and this shall be recorded by the DCO .
C.4.4 The Athlete shall retain control of the collection vessel and any Sample provided until the Sample (or partial Sample) is sealed, unless assistance is required by reason of an Athlete’ s impairment as provided for in Annex A - Modifications for Athletes with Impairment s. Additional assistance may be provided in exceptional circumstances to any Athlete by the Athlete’s representative or Sample Collection Personnel during the Sample Collection Session where authorized by the Athlete and agreed to by the DCO .
C.4.5 The DCO /Chaperone who witnesses the passing of the Sample shall be of the same gender as the Athlete providing the Sample and where applicable, based on the gender of the Event the Athlete competed in.
C.4.6 The DCO /Chaperone shall, where practicable, ensure the Athlete thoroughly washes their hands with water only prior to the provision of the Sample or wears suitable (e.g., disposable) gloves during provision of the Sample .
C.4.7 The DCO /Chaperone and Athlete shall proceed to an area of privacy to collect a Sample .
C.4.8 The DCO /Chaperone shall ensure an unobstructed view of the Sample leaving the Athlete’ s body and shall continue to observe the Sample after provision until the Sample is securely sealed.
In order to ensure a clear and unobstructed view of the passing of the Sample, the DCO /Chaperone shall instruct the Athlete to remove or adjust any clothing which restricts the DCO’s /Chaperone’s clear view of Sample provision.
C.4.9 The DCO /Chaperone shall ensure that urine passed by the Athlete is collected in the collection vessel to its maximum capacity and thereafter the Athlete is encouraged to fully empty their bladder into the toilet.
The DCO shall verify, in full view of the Athlete, that the Suitable Volume of Urine for Analysis has been provided.
C.4.10 Where the volume of urine provided by the Athlete is insufficient, the DCO shall follow the partial Sample collection procedure set out in Annex E - Urine Samples - Insufficient Volume.
ISTI – Effective 1 January 2023 Page 67 of 92 C.4.11 Once the volume of urine provided by the Athlete is sufficient, the DCO shall instruct the Athlete to select a Sample collection kit containing A and B bottles or containers in accordance with Annex C.4.3.
C.4.12 Once a Sample collection kit has been selected, the DCO and the Athlete shall check that all Sample code numbers match and that this code number is recorded accurately by the DCO on the Doping Control form.
If the Athlete or DCO finds that the numbers are not the same, the DCO shall instruct the Athlete to choose another kit in accordance with Annex C.4.3.
The DCO shall record the matter.
C.4.13 The Athlete shall pour the minimum Suitable Volume of Urine for Analysis into the B bottle or container (to a minimum of 30 mL), and then pour the remainder of the urine into the A bottle or container (to a minimum of 60 mL).
The Suitable Volume of Urine for Analysis shall be viewed as an absolute minimum.
If more than the minimum Suitable Volume of Urine for Analysis has been provided, the DCO shall ensure that the Athlete fills the A bottle or container to capacity as per the recommendation of the equipment manufacturer.
Should there still be urine remaining, the DCO shall ensure that the Athlete fills the B bottle or container to capacity as per the recommendation of the equipment manufacturer.
The DCO shall instruct the Athlete to ensure that a small amount of urine is left in the collection vessel, explaining that this is to enable the DCO to test the residual urine in accordance with Annex C.4.15.
C.4.14 The Athlete shall then seal the A and B bottles or containers as directed by the DCO .
The DCO shall check, in full view of the Athlete , that the bottles or containers have been properly sealed.
C.4.15 The DCO shall test the residual urine in the collection vessel to determine if the Sample has a Suitable Specific Gravity for Analysis .
If the DCO’s field reading indicates that the Sample does not have a Suitable Specific Gravity for Analysis , then the DCO shall follow Annex F - Urine Samples that do not meet the requirement for Suitable Specific Gravity for Analysis .
C.4.16 Urine should only be discarded when both the A and B bottles or containers have been sealed and the residual urine has been tested in accordance with Annex C.4.15.
C.4.17 The Athlete shall be given the option of witnessing the discarding of any residual urine that will not be sent for analysis.
ISTI – Effective 1 January 2023 Page 68 of 92 ANNEX D - COLLECTION OF VENOUS BLOOD SAMPLES D.1 Objective To collect an Athlete’ s blood Sample by venipuncture in a manner that ensures: a) Consistency with relevant principles of internationally recogni zed standard precautions in healthcare settings, and is collected by a suitably qualified Person, so that the health and safety of the Athlete and Sample Collection Personnel are not compromised; b) The Sample is of a quality and quantity that meets the relevant analytical guidelines and requirements defined by the Laboratory ; c) The Sample has not been manipulated, substituted, contaminated or otherwise tampered with in any way; d) The Sample is clearly and accurately identified; and e) The Sample is securely sealed in a Tamper Evident kit.
D.2 Scope The requirements of this Annex apply to venous blood Samples collected for the purposes of specific analysis and/or all modules of the At hlete Biological Passport .
The collection of a venous blood Sample begins with ensuring the Athlete is informed of the Sample collection requirements and ends with properly storing the Sample prior to transport to the Laboratory that will be analy zing the Sample.
[Comment to D.2: Additional requirements applicable only to whole blood Samples collected for the hematological module of the Athlete Biological Passport are contained in Annex I - Collection, Storage and Transport of Blood Athlete Biological Passport Samples and, requirements for dried blood spot Samples are contained in Annex J - Collection, Storage and Transport of Dried Blood Spot Samples.]
D.3 Responsibility D.3.1 The DCO has the responsibility for ensuring that: a) Each Sample is properly collected, identified and sealed; and b) All Samples have been properly stored and dispatched in accordance with the relevant analytical guidelines.
D.3.2 The BCO has the responsibility for collecting the blood Sample , answering related questions during the provision of the Sample , and proper disposal of used blood sampling equipment not required to complete the Sample Collection Session.
D.4 Requirements D.4.1 Procedures involving blood shall be consistent with the local standards and regulatory requirements regarding precautions in healthcare settings where those standards and requirements exceed the requirements set out below.
ISTI – Effective 1 January 2023 Page 69 of 92 D.4.2 Blood Sample Collection Equipment shall consist of: a) Collection tube(s); and/or b) A and B bottles /containers for the secure transportation of collection tube( s); and/or c) Unique labels for collection tube (s) with a Sample code number; and/or d) Such other types of equipment to be used in connection with the collection of blood as set out in Article 6.3.4 and WADA's Guidelines for Sample Collection.
D.4.3 The DCO shall ensure that the Athlete is properly notified of the requirements of the Sample collection, including any modifications as provided for in Annex A - Modifications for Athletes with Impairments.
D.4.4 The DCO /Chaperone and Athlete shall proceed to the area where the Sample will be provided.
D.4.5 The DCO /BCO shall ensure the Athlete is offered comfortable conditions and shall instruct the Athlete to remain in an upright , stationary seated position with feet on the floor for at least 10 minutes prior to providing a blood Sample.
If the Athlete’s feet cannot reach t he floor and/or the Athlete’s impairment does not allow feet on the floor , the Athlete shall remain in an upright , stationary seated position .
D.4.6 The DCO /BCO shall instruct the Athlete to select the Sample Collection Equipment required for collecting the Sample and to check that the selected equipment has not been tampered with and any seals are intact.
If the Athlete is not satisfied with the selected equipment , they may select another.
If the Athlete is not satisfied with any equipment and no other is available, this shall be recorded by the DCO .
If the DCO does not agree with the Athlete that all of the available equipment is unsatisfactory, the DCO shall instruct the Athlete to proceed with the Sample Collection Session .
If the DCO agrees with the Athlete that all available equipment is unsatisfactory, the DCO shall terminate the blood Sample collection, and this shall be recorded by the DCO .
D.4.7 When a Sample collection kit has been selected, the DCO and the Athlete shall check that all Sample code numbers match and that this Sample code number is recorded accurately by the DCO on the Doping Control form.
If the Athlete or DCO finds that the numbers are not the same, the DCO shall instruct the Athlete to choose another kit.
The DCO shall record the matter.
If the collection tube(s) are not pre -labelled, the DCO /BCO shall label them with a unique Sample code number prior to the blood being drawn and the Athlete shall check that the code numbers match.
D.4.8 The BCO shall assess the most suitable location for venipuncture that is unlikely to adversely affect the Athlete or their performance.
This should be the non -dominant arm, unless the BCO assesses the other arm to be more suitable.
The BCO shall clean the skin with a sterile disinfectant wipe or swab and, if required apply a tourniquet.
The BCO shall take the blood Sample from a superficial vein into the tube.
The tourniquet, if applied, shall be immedi ately removed after the venipuncture has been made.
ISTI – Effective 1 January 2023 Page 70 of 92 D.4.9 The amount of blood removed shall be adequate to satisfy the relevant analytical requirements for the Sample analysis to be performed, as set out in WADA’s Guidelines for Sample Collection.
D.4.10 If the amount of blood that can be removed from the Athlete at the first attempt is insufficient, the BCO shall repeat the procedure up to a maximum of three (3) attempts in total.
Should all three (3) attempts fail to produce a suffici ent amount of blood, then the BCO shall inform the DCO .
The DCO shall terminate the blood Sample collection and record the reasons for terminating.
D.4.11 The BCO shall apply a dressing to the puncture site(s).
D.4.12 The BCO shall dispose of used blood sampling equipment not required to complete the Sample Collection Session in accordance with the required local standards for handling blood.
D.4.13 After the blood flow into the tube ceases, the BCO shall remove the tube from the holder and homogenize the blood in the tube manually by inverting the tube gently at least three (3) times).
The Athlete shall remain in the blood collection area and observe thei r Sample until it is sealed in a Tamper Evident kit.
D.4.14 The Athlete shall seal their Sample into a Tamper Evident kit as directed by the DCO .
In full view of the Athlete , the DCO shall check that the sealing is satisfactory.
The Athlete and the BCO /DCO shall sign the Doping Control form.
D.4.15 The sealed Sample shall be stored in a manner that protects its integrity, identity and security prior to transport from the Doping Control Station to the Laboratory that will be analy zing the Sample .