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Typically though, only a few residues contribute to most of the binding energy. The existence of two identical antibody-binding sites allows antibody molecules to bind strongly to multivalent antigen (repeating sites such as polysaccharides in bacterial cell walls, or other sites at some distance apart), as well as to form antibody complexes and larger antigen-antibody complexes. The structures of CDRs have been clustered and classified by Chothia et al. and more recently by North et al. and Nikoloudis et al. However, describing an antibody's binding site using only one single static structure limits the understanding and characterization of the antibody's function and properties. To improve antibody structure prediction and to take the strongly correlated CDR loop and interface movements into account, antibody paratopes should be described as interconverting states in solution with varying probabilities. In the framework of the immune network theory, CDRs are also called idiotypes. According to immune network theory, the adaptive immune system is regulated by interactions between idiotypes.
Fc region. The Fc region (the trunk of the Y shape) is composed of constant domains from the heavy chains. Its role is in modulating immune cell activity: it is where effector molecules bind to, triggering various effects after the antibody Fab region binds to an antigen. Effector cells (such as macrophages or natural killer cells) bind via their Fc receptors (FcR) to the Fc region of an antibody, while the complement system is activated by binding the C1q protein complex. IgG or IgM can bind to C1q, but IgA cannot, therefore IgA does not activate the classical complement pathway. Another role of the Fc region is to selectively distribute different antibody classes across the body. In particular, the neonatal Fc receptor (FcRn) binds to the Fc region of IgG antibodies to transport it across the placenta, from the mother to the fetus. In addition to this, binding to FcRn endows IgG with an exceptionally long half-life relative to other plasma proteins of 3-4 weeks. IgG3 in most cases (depending on allotype) has mutations at the FcRn binding site which lower affinity for FcRn, which are thought to have evolved to limit the highly inflammatory effects of this subclass.
Antibodies are glycoproteins, that is, they have carbohydrates (glycans) added to conserved amino acid residues. These conserved glycosylation sites occur in the Fc region and influence interactions with effector molecules. Protein structure. The N-terminus of each chain is situated at the tip. Each immunoglobulin domain has a similar structure, characteristic of all the members of the immunoglobulin superfamily: it is composed of between 7 (for constant domains) and 9 (for variable domains) β-strands, forming two beta sheets in a Greek key motif. The sheets create a "sandwich" shape, the immunoglobulin fold, held together by a disulfide bond. Antibody complexes. Secreted antibodies can occur as a single Y-shaped unit, a monomer. However, some antibody classes also form dimers with two Ig units (as with IgA), tetramers with four Ig units (like teleost fish IgM), or pentamers with five Ig units (like shark IgW or mammalian IgM, which occasionally forms hexamers as well, with six units). IgG can also form hexamers, though no J chain is required. IgA tetramers and pentamers have also been reported.
Antibodies also form complexes by binding to antigen: this is called an antigen-antibody complex or "immune complex". Small antigens can cross-link two antibodies, also leading to the formation of antibody dimers, trimers, tetramers, etc. Multivalent antigens (e.g., cells with multiple epitopes) can form larger complexes with antibodies. An extreme example is the clumping, or agglutination, of red blood cells with antibodies in blood typing to determine blood groups: the large clumps become insoluble, leading to visually apparent precipitation. B cell receptors. The membrane-bound form of an antibody may be called a "surface immunoglobulin" (sIg) or a "membrane immunoglobulin" (mIg). It is part of the "B cell receptor" (BCR), which allows a B cell to detect when a specific antigen is present in the body and triggers B cell activation. The BCR is composed of surface-bound IgD or IgM antibodies and associated Ig-α and Ig-β heterodimers, which are capable of signal transduction. A typical human B cell will have 50,000 to 100,000 antibodies bound to its surface. Upon antigen binding, they cluster in large patches, which can exceed 1 micrometer in diameter, on lipid rafts that isolate the BCRs from most other cell signaling receptors.
These patches may improve the efficiency of the cellular immune response. In humans, the cell surface is bare around the B cell receptors for several hundred nanometers, which further isolates the BCRs from competing influences. Classes. Antibodies can come in different varieties known as "isotypes" or "classes". In humans there are five antibody classes known as IgA, IgD, IgE, IgG, and IgM, which are further subdivided into subclasses such as IgA1, IgA2. The prefix "Ig" stands for "immunoglobulin", while the suffix denotes the type of heavy chain the antibody contains: the heavy chain types α (alpha), γ (gamma), δ (delta), ε (epsilon), μ (mu) give rise to IgA, IgG, IgD, IgE, IgM, respectively. The distinctive features of each class are determined by the part of the heavy chain within the hinge and Fc region. The classes differ in their biological properties, functional locations and ability to deal with different antigens, as depicted in the table. For example, IgE antibodies are responsible for an allergic response consisting of histamine release from mast cells, often a sole contributor to asthma (though other pathways exist as do symptoms very similar to yet not technically asthma). The variable region of these antibodies bind to allergic antigen, for example house dust mite particles, while its Fc region (in the ε heavy chains) binds to Fc receptor ε on a mast cell, triggering its degranulation: the release of molecules stored in its granules.
The antibody isotype of a B cell changes during cell development and activation. Immature B cells, which have never been exposed to an antigen, express only the IgM isotype in a cell surface bound form. The B lymphocyte, in this ready-to-respond form, is known as a "naive B lymphocyte." The naive B lymphocyte expresses both surface IgM and IgD. The co-expression of both of these immunoglobulin isotypes renders the B cell ready to respond to antigen. B cell activation follows engagement of the cell-bound antibody molecule with an antigen, causing the cell to divide and differentiate into an antibody-producing cell called a plasma cell. This requires cytokines from T helper cells, unless antigen cross-links B cell receptors. In this activated form, the B cell starts to produce antibody in a secreted form rather than a membrane-bound form. Activated B cells that encounter certain signaling molecules undergo immunoglobulin class switching, also known as isotope switching, which causes the production of antibodies to change from IgM or IgD to the other antibody isotypes, IgE, IgA, or IgG.
Light chain types. In mammals there are two types of immunoglobulin light chain, which are called lambda (λ) and kappa (κ). However, there is no known functional difference between them, and both can occur with any of the five major types of heavy chains. Each antibody contains two identical light chains: both κ or both λ. Proportions of κ and λ types vary by species and can be used to detect abnormal proliferation of B cell clones. Other types of light chains, such as the iota (ι) chain, are found in other vertebrates like sharks (Chondrichthyes) and bony fishes (Teleostei). In non-mammalian animals. In most placental mammals, the structure of antibodies is generally the same. Jawed fish appear to be the most primitive animals that are able to make antibodies similar to those of mammals, although many features of their adaptive immunity appeared somewhat earlier. Cartilaginous fish (such as sharks) produce heavy-chain-only antibodies (i.e., lacking light chains) which moreover feature longer chain pentamers (with five constant units per molecule). Camelids (such as camels, llamas, alpacas) are also notable for producing heavy-chain-only antibodies.
Antibody–antigen interactions. The antibody's paratope interacts with the antigen's epitope. An antigen usually contains different epitopes along its surface arranged discontinuously, and dominant epitopes on a given antigen are called determinants. Antibody and antigen interact by spatial complementarity (lock and key). The molecular forces involved in the Fab-epitope interaction are weak and non-specific – for example electrostatic forces, hydrogen bonds, hydrophobic interactions, and van der Waals forces. This means binding between antibody and antigen is reversible, and the antibody's affinity towards an antigen is relative rather than absolute. Relatively weak binding also means it is possible for an antibody to cross-react with different antigens of different relative affinities. Function. The main categories of antibody action include the following: More indirectly, an antibody can signal immune cells to present antibody fragments to T cells, or downregulate other immune cells to avoid autoimmunity. Activated B cells differentiate into either
At the prenatal and neonatal stages of life, the presence of antibodies is provided by passive immunization from the mother. Early endogenous antibody production varies for different kinds of antibodies, and usually appear within the first years of life. Since antibodies exist freely in the bloodstream, they are said to be part of the humoral immune system. Circulating antibodies are produced by clonal B cells that specifically respond to only one antigen (an example is a virus capsid protein fragment). Antibodies contribute to immunity in three ways: They prevent pathogens from entering or damaging cells by binding to them; they stimulate removal of pathogens by macrophages and other cells by coating the pathogen; and they trigger destruction of pathogens by stimulating other immune responses such as the complement pathway. Antibodies will also trigger vasoactive amine degranulation to contribute to immunity against certain types of antigens (helminths, allergens). Activation of complement. Antibodies that bind to surface antigens (for example, on bacteria) will attract the first component of the complement cascade with their Fc region and initiate activation of the "classical" complement system. This results in the killing of bacteria in two ways. First, the binding of the antibody and complement molecules marks the microbe for ingestion by phagocytes in a process called opsonization; these phagocytes are attracted by certain complement molecules generated in the complement cascade. Second, some complement system components form a membrane attack complex to assist antibodies to kill the bacterium directly (bacteriolysis).
Activation of effector cells. To combat pathogens that replicate outside cells, antibodies bind to pathogens to link them together, causing them to agglutinate. Since an antibody has at least two paratopes, it can bind more than one antigen by binding identical epitopes carried on the surfaces of these antigens. By coating the pathogen, antibodies stimulate effector functions against the pathogen in cells that recognize their Fc region. Those cells that recognize coated pathogens have Fc receptors, which, as the name suggests, interact with the Fc region of IgA, IgG, and IgE antibodies. The engagement of a particular antibody with the Fc receptor on a particular cell triggers an effector function of that cell; phagocytes will phagocytose, mast cells and neutrophils will degranulate, natural killer cells will release cytokines and cytotoxic molecules; that will ultimately result in destruction of the invading microbe. The activation of natural killer cells by antibodies initiates a cytotoxic mechanism known as antibody-dependent cell-mediated cytotoxicity (ADCC) – this process may explain the efficacy of monoclonal antibodies used in biological therapies against cancer. The Fc receptors are isotype-specific, which gives greater flexibility to the immune system, invoking only the appropriate immune mechanisms for distinct pathogens.
Natural antibodies. Humans and higher primates also produce "natural antibodies" that are present in serum before viral infection. Natural antibodies have been defined as antibodies that are produced without any previous infection, vaccination, other foreign antigen exposure or passive immunization. These antibodies can activate the classical complement pathway leading to lysis of enveloped virus particles long before the adaptive immune response is activated. Antibodies are produced exclusively by B cells in response to antigens where initially, antibodies are formed as membrane-bound receptors, but upon activation by antigens and helper T cells, B cells differentiate to produce soluble antibodies. Many natural antibodies are directed against the disaccharide galactose α(1,3)-galactose (α-Gal), which is found as a terminal sugar on glycosylated cell surface proteins, and generated in response to production of this sugar by bacteria contained in the human gut. These antibodies undergo quality checks in the endoplasmic reticulum (ER), which contains proteins that assist in proper folding and assembly. Rejection of xenotransplantated organs is thought to be, in part, the result of natural antibodies circulating in the serum of the recipient binding to α-Gal antigens expressed on the donor tissue.
Immunoglobulin diversity. Virtually all microbes can trigger an antibody response. Successful recognition and eradication of many different types of microbes requires diversity among antibodies; their amino acid composition varies allowing them to interact with many different antigens. It has been estimated that humans generate about 10 billion different antibodies, each capable of binding a distinct epitope of an antigen. Although a huge repertoire of different antibodies is generated in a single individual, the number of genes available to make these proteins is limited by the size of the human genome. Several complex genetic mechanisms have evolved that allow vertebrate B cells to generate a diverse pool of antibodies from a relatively small number of antibody genes. Domain variability. The chromosomal region that encodes an antibody is large and contains several distinct gene loci for each domain of the antibody—the chromosome region containing heavy chain genes (IGH@) is found on chromosome 14, and the loci containing lambda and kappa light chain genes (IGL@ and IGK@) are found on chromosomes 22 and 2 in humans. One of these domains is called the variable domain, which is present in each heavy and light chain of every antibody, but can differ in different antibodies generated from distinct B cells. Differences between the variable domains are located on three loops known as hypervariable regions (HV-1, HV-2 and HV-3) or complementarity-determining regions (CDR1, CDR2 and CDR3). CDRs are supported within the variable domains by conserved framework regions. The heavy chain locus contains about 65 different variable domain genes that all differ in their CDRs. Combining these genes with an array of genes for other domains of the antibody generates a large cavalry of antibodies with a high degree of variability. This combination is called V(D)J recombination and discussed below.
V(D)J recombination. Somatic recombination of immunoglobulins, also known as "V(D)J recombination", involves the generation of a unique immunoglobulin variable region. The variable region of each immunoglobulin heavy or light chain is encoded in several pieces—known as gene segments (subgenes). These segments are called variable (V), diversity (D) and joining (J) segments. V, D and J segments are found in Ig heavy chains, but only V and J segments are found in Ig light chains. Multiple copies of the V, D and J gene segments exist, and are tandemly arranged in the genomes of mammals. In the bone marrow, each developing B cell will assemble an immunoglobulin variable region by randomly selecting and combining one V, one D and one J gene segment (or one V and one J segment in the light chain). As there are multiple copies of each type of gene segment, and different combinations of gene segments can be used to generate each immunoglobulin variable region, this process generates a huge number of antibodies, each with different paratopes, and thus different antigen specificities. The rearrangement of several subgenes (i.e. V2 family) for lambda light chain immunoglobulin is coupled with the activation of microRNA miR-650, which further influences biology of B-cells.
RAG proteins play an important role with V(D)J recombination in cutting DNA at a particular region. Without the presence of these proteins, V(D)J recombination would not occur. After a B cell produces a functional immunoglobulin gene during V(D)J recombination, it cannot express any other variable region (a process known as allelic exclusion) thus each B cell can produce antibodies containing only one kind of variable chain. Somatic hypermutation and affinity maturation. Following activation with antigen, B cells begin to proliferate rapidly. In these rapidly dividing cells, the genes encoding the variable domains of the heavy and light chains undergo a high rate of point mutation, by a process called "somatic hypermutation" (SHM). SHM results in approximately one nucleotide change per variable gene, per cell division. As a consequence, any daughter B cells will acquire slight amino acid differences in the variable domains of their antibody chains. This serves to increase the diversity of the antibody pool and impacts the antibody's antigen-binding affinity. Some point mutations will result in the production of antibodies that have a weaker interaction (low affinity) with their antigen than the original antibody, and some mutations will generate antibodies with a stronger interaction (high affinity). B cells that express high affinity antibodies on their surface will receive a strong survival signal during interactions with other cells, whereas those with low affinity antibodies will not, and will die by apoptosis. Thus, B cells expressing antibodies with a higher affinity for the antigen will outcompete those with weaker affinities for function and survival allowing the average affinity of antibodies to increase over time. The process of generating antibodies with increased binding affinities is called "affinity maturation". Affinity maturation occurs in mature B cells after V(D)J recombination, and is dependent on help from helper T cells.
Class switching. Isotype or class switching is a biological process occurring after activation of the B cell, which allows the cell to produce different classes of antibody (IgA, IgE, or IgG). The different classes of antibody, and thus effector functions, are defined by the constant (C) regions of the immunoglobulin heavy chain. Initially, naive B cells express only cell-surface IgM and IgD with identical antigen binding regions. Each isotype is adapted for a distinct function; therefore, after activation, an antibody with an IgG, IgA, or IgE effector function might be required to effectively eliminate an antigen. Class switching allows different daughter cells from the same activated B cell to produce antibodies of different isotypes. Only the constant region of the antibody heavy chain changes during class switching; the variable regions, and therefore antigen specificity, remain unchanged. Thus the progeny of a single B cell can produce antibodies, all specific for the same antigen, but with the ability to produce the effector function appropriate for each antigenic challenge. Class switching is triggered by cytokines; the isotype generated depends on which cytokines are present in the B cell environment.
Class switching occurs in the heavy chain gene locus by a mechanism called class switch recombination (CSR). This mechanism relies on conserved nucleotide motifs, called "switch (S) regions", found in DNA upstream of each constant region gene (except in the δ-chain). The DNA strand is broken by the activity of a series of enzymes at two selected S-regions. The variable domain exon is rejoined through a process called non-homologous end joining (NHEJ) to the desired constant region (γ, α or ε). This process results in an immunoglobulin gene that encodes an antibody of a different isotype. Specificity designations. An antibody can be called "monospecific" if it has specificity for a single antigen or epitope, or bispecific if it has affinity for two different antigens or two different epitopes on the same antigen. A group of antibodies can be called "polyvalent" (or "unspecific") if they have affinity for various antigens or microorganisms. Intravenous immunoglobulin, if not otherwise noted, consists of a variety of different IgG (polyclonal IgG). In contrast, monoclonal antibodies are identical antibodies produced by a single B cell.
Asymmetrical antibodies. Heterodimeric antibodies, which are also asymmetrical antibodies, allow for greater flexibility and new formats for attaching a variety of drugs to the antibody arms. One of the general formats for a heterodimeric antibody is the "knobs-into-holes" format. This format is specific to the heavy chain part of the constant region in antibodies. The "knobs" part is engineered by replacing a small amino acid with a larger one. It fits into the "hole", which is engineered by replacing a large amino acid with a smaller one. What connects the "knobs" to the "holes" are the disulfide bonds between each chain. The "knobs-into-holes" shape facilitates antibody dependent cell mediated cytotoxicity. Single-chain variable fragments (scFv) are connected to the variable domain of the heavy and light chain via a short linker peptide. The linker is rich in glycine, which gives it more flexibility, and serine/threonine, which gives it specificity. Two different scFv fragments can be connected together, via a hinge region, to the constant domain of the heavy chain or the constant domain of the light chain. This gives the antibody bispecificity, allowing for the binding specificities of two different antigens. The "knobs-into-holes" format enhances heterodimer formation but does not suppress homodimer formation.
To further improve the function of heterodimeric antibodies, many scientists are looking towards artificial constructs. Artificial antibodies are largely diverse protein motifs that use the functional strategy of the antibody molecule, but are not limited by the loop and framework structural constraints of the natural antibody. Being able to control the combinational design of the sequence and three-dimensional space could transcend the natural design and allow for the attachment of different combinations of drugs to the arms. Heterodimeric antibodies have a greater range in shapes they can take and the drugs that are attached to the arms do not have to be the same on each arm, allowing for different combinations of drugs to be used in cancer treatment. Pharmaceuticals are able to produce highly functional bispecific, and even multispecific, antibodies. The degree to which they can function is impressive given that such a change of shape from the natural form should lead to decreased functionality. Interchromosomal DNA Transposition.
Antibody diversification typically occurs through somatic hypermutation, class switching, and affinity maturation targeting the BCR gene loci, but on occasion more unconventional forms of diversification have been documented. For example, in the case of malaria caused by "Plasmodium falciparum," some antibodies from those who had been infected demonstrated an insertion from chromosome 19 containing a 98-amino acid stretch from leukocyte-associated immunoglobulin-like receptor 1, LAIR1, in the elbow joint. This represents a form of interchromosomal transposition. LAIR1 normally binds collagen, but can recognize repetitive interspersed families of polypeptides (RIFIN) family members that are highly expressed on the surface of "P. falciparum"-infected red blood cells. In fact, these antibodies underwent affinity maturation that enhanced affinity for RIFIN but abolished affinity for collagen. These "LAIR1-containing" antibodies have been found in 5-10% of donors from Tanzania and Mali, though not in European donors. European donors did show 100-1000 nucleotide stretches inside the elbow joints as well, however. This particular phenomenon may be specific to malaria, as infection is known to induce genomic instability.
History. The first use of the term "antibody" occurred in a text by Paul Ehrlich. The term "Antikörper" (the German word for "antibody") appears in the conclusion of his article "Experimental Studies on Immunity", published in October 1891, which states that, "if two substances give rise to two different "Antikörper", then they themselves must be different". However, the term was not accepted immediately and several other terms for antibody were proposed; these included "Immunkörper", "Amboceptor", "Zwischenkörper", "substance sensibilisatrice", "copula", "Desmon", "philocytase", "fixateur", and "Immunisin". The word "antibody" has formal analogy to the word "antitoxin" and a similar concept to "Immunkörper" ("immune body" in English). As such, the original construction of the word contains a logical flaw; the antitoxin is something directed against a toxin, while the antibody is a body directed against something. The study of antibodies began in 1890 when Emil von Behring and Kitasato Shibasaburō described antibody activity against diphtheria and tetanus toxins. Von Behring and Kitasato put forward the theory of humoral immunity, proposing that a mediator in serum could react with a foreign antigen. His idea prompted Paul Ehrlich to propose the side-chain theory for antibody and antigen interaction in 1897, when he hypothesized that receptors (described as "side-chains") on the surface of cells could bind specifically to toxins – in a "lock-and-key" interaction – and that this binding reaction is the trigger for the production of antibodies. Other researchers believed that antibodies existed freely in the blood and, in 1904, Almroth Wright suggested that soluble antibodies coated bacteria to label them for phagocytosis and killing; a process that he named opsoninization.
In the 1920s, Michael Heidelberger and Oswald Avery observed that antigens could be precipitated by antibodies and went on to show that antibodies are made of protein. The biochemical properties of antigen-antibody-binding interactions were examined in more detail in the late 1930s by John Marrack. The next major advance was in the 1940s, when Linus Pauling confirmed the lock-and-key theory proposed by Ehrlich by showing that the interactions between antibodies and antigens depend more on their shape than their chemical composition. In 1948, Astrid Fagraeus discovered that B cells, in the form of plasma cells, were responsible for generating antibodies. Further work concentrated on characterizing the structures of the antibody proteins. A major advance in these structural studies was the discovery in the early 1960s by Gerald Edelman and Joseph Gally of the antibody light chain, and their realization that this protein is the same as the Bence-Jones protein described in 1845 by Henry Bence Jones. Edelman went on to discover that antibodies are composed of disulfide bond-linked heavy and light chains. Around the same time, antibody-binding (Fab) and antibody tail (Fc) regions of IgG were characterized by Rodney Porter. Together, these scientists deduced the structure and complete amino acid sequence of IgG, a feat for which they were jointly awarded the 1972 Nobel Prize in Physiology or Medicine. The Fv fragment was prepared and characterized by David Givol. While most of these early studies focused on IgM and IgG, other immunoglobulin isotypes were identified in the 1960s: Thomas Tomasi discovered secretory antibody (IgA); David S. Rowe and John L. Fahey discovered IgD; and Kimishige Ishizaka and Teruko Ishizaka discovered IgE and showed it was a class of antibodies involved in allergic reactions. In a landmark series of experiments beginning in 1976, Susumu Tonegawa showed that genetic material can rearrange itself to form the vast array of available antibodies.
Medical applications. Disease diagnosis. Detection of particular antibodies is a very common form of medical diagnostics, and applications such as serology depend on these methods. For example, in biochemical assays for disease diagnosis, a titer of antibodies directed against Epstein-Barr virus or Lyme disease is estimated from the blood. If those antibodies are not present, either the person is not infected or the infection occurred a "very" long time ago, and the B cells generating these specific antibodies have naturally decayed. In clinical immunology, levels of individual classes of immunoglobulins are measured by nephelometry (or turbidimetry) to characterize the antibody profile of patient. Elevations in different classes of immunoglobulins are sometimes useful in determining the cause of liver damage in patients for whom the diagnosis is unclear. For example, IgM levels are often elevated in patients with primary biliary cirrhosis, whereas IgA deposition along hepatic sinusoids can suggest alcoholic liver disease.
Autoimmune disorders can often be traced to antibodies that bind the body's own epitopes; many can be detected through blood tests. Antibodies directed against red blood cell surface antigens in immune mediated hemolytic anemia are detected with the Coombs test. The Coombs test is also used for antibody screening in blood transfusion preparation and also for antibody screening in antenatal women. Practically, several immunodiagnostic methods based on detection of complex antigen-antibody are used to diagnose infectious diseases, for example ELISA, immunofluorescence, Western blot, immunodiffusion, immunoelectrophoresis, and magnetic immunoassay. Over-the-counter home pregnancy tests rely on human chorionic gonadotropin (hCG)-directed antibodies. New dioxaborolane chemistry enables radioactive fluoride (18F) labeling of antibodies, which allows for positron emission tomography (PET) imaging of cancer. Disease therapy. Targeted monoclonal antibody therapy is employed to treat diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, and many forms of cancer including non-Hodgkin's lymphoma, colorectal cancer, head and neck cancer and breast cancer.
Some immune deficiencies, such as X-linked agammaglobulinemia and hypogammaglobulinemia, result in partial or complete lack of antibodies. These diseases are often treated by inducing a short-term form of immunity called passive immunity. Passive immunity is achieved through the transfer of ready-made antibodies in the form of human or animal serum, pooled immunoglobulin or monoclonal antibodies, into the affected individual. Prenatal therapy. Rh factor, also known as Rh D antigen, is an antigen found on red blood cells; individuals that are Rh-positive (Rh+) have this antigen on their red blood cells and individuals that are Rh-negative (Rh–) do not. During normal childbirth, delivery trauma or complications during pregnancy, blood from a fetus can enter the mother's system. In the case of an Rh-incompatible mother and child, consequential blood mixing may sensitize an Rh- mother to the Rh antigen on the blood cells of the Rh+ child, putting the remainder of the pregnancy, and any subsequent pregnancies, at risk for hemolytic disease of the newborn.
Rho(D) immune globulin antibodies are specific for human RhD antigen. Anti-RhD antibodies are administered as part of a prenatal treatment regimen to prevent sensitization that may occur when a Rh-negative mother has a Rh-positive fetus. Treatment of a mother with Anti-RhD antibodies prior to and immediately after trauma and delivery destroys Rh antigen in the mother's system from the fetus. This occurs before the antigen can stimulate maternal B cells to "remember" Rh antigen by generating memory B cells. Therefore, her humoral immune system will not make anti-Rh antibodies, and will not attack the Rh antigens of the current or subsequent babies. Rho(D) Immune Globulin treatment prevents sensitization that can lead to Rh disease, but does not prevent or treat the underlying disease itself. Research applications. Specific antibodies are produced by injecting an antigen into a mammal, such as a mouse, rat, rabbit, goat, sheep, or horse for large quantities of antibody. Blood isolated from these animals contains "polyclonal antibodies"—multiple antibodies that bind to the same antigen—in the serum, which can now be called antiserum. Antigens are also injected into chickens for generation of polyclonal antibodies in egg yolk. To obtain antibody that is specific for a single epitope of an antigen, antibody-secreting lymphocytes are isolated from the animal and immortalized by fusing them with a cancer cell line. The fused cells are called hybridomas, and will continually grow and secrete antibody in culture. Single hybridoma cells are isolated by dilution cloning to generate cell clones that all produce the same antibody; these antibodies are called "monoclonal antibodies". Polyclonal and monoclonal antibodies are often purified using Protein A/G or antigen-affinity chromatography.
In research, purified antibodies are used in many applications. Antibodies for research applications can be found directly from antibody suppliers, or through use of a specialist search engine. Research antibodies are most commonly used to identify and locate intracellular and extracellular proteins. Antibodies are used in flow cytometry to differentiate cell types by the proteins they express; different types of cells express different combinations of cluster of differentiation molecules on their surface, and produce different intracellular and secretable proteins. They are also used in immunoprecipitation to separate proteins and anything bound to them (co-immunoprecipitation) from other molecules in a cell lysate, in Western blot analyses to identify proteins separated by electrophoresis, and in immunohistochemistry or immunofluorescence to examine protein expression in tissue sections or to locate proteins within cells with the assistance of a microscope. Proteins can also be detected and quantified with antibodies, using ELISA and ELISpot techniques.
Antibodies used in research are some of the most powerful, yet most problematic reagents with a tremendous number of factors that must be controlled in any experiment including cross reactivity, or the antibody recognizing multiple epitopes and affinity, which can vary widely depending on experimental conditions such as pH, solvent, state of tissue etc. Multiple attempts have been made to improve both the way that researchers validate antibodies and ways in which they report on antibodies. Researchers using antibodies in their work need to record them correctly in order to allow their research to be reproducible (and therefore tested, and qualified by other researchers). Less than half of research antibodies referenced in academic papers can be easily identified. Papers published in F1000 in 2014 and 2015 provide researchers with a guide for reporting research antibody use. The RRID paper, is co-published in 4 journals that implemented the RRIDs Standard for research resource citation, which draws data from the antibodyregistry.org as the source of antibody identifiers (see also group at Force11).
Antibody regions can be used to further biomedical research by acting as a guide for drugs to reach their target. Several application involve using bacterial plasmids to tag plasmids with the Fc region of the antibody such as pFUSE-Fc plasmid. Regulations. Production and testing. There are several ways to obtain antibodies, including in vivo techniques like animal immunization and various in vitro approaches, such as the phage display method. Traditionally, most antibodies are produced by hybridoma cell lines through immortalization of antibody-producing cells by chemically induced fusion with myeloma cells. In some cases, additional fusions with other lines have created "triomas" and "quadromas". The manufacturing process should be appropriately described and validated. Validation studies should at least include: Structure prediction and computational antibody design.
The ability to describe the antibody through binding affinity to the antigen is supplemented by information on antibody structure and amino acid sequences for the purpose of patent claims. Several methods have been presented for computational design of antibodies based on the structural bioinformatics studies of antibody CDRs. There are a variety of methods used to sequence an antibody including Edman degradation, cDNA, etc.; albeit one of the most common modern uses for peptide/protein identification is liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). High volume antibody sequencing methods require computational approaches for the data analysis, including de novo sequencing directly from tandem mass spectra and database search methods that use existing protein sequence databases. Many versions of shotgun protein sequencing are able to increase the coverage by utilizing CID/HCD/ETD fragmentation methods and other techniques, and they have achieved substantial progress in attempt to fully sequence proteins, especially antibodies. Other methods have assumed the existence of similar proteins, a known genome sequence, or combined top-down and bottom up approaches. Current technologies have the ability to assemble protein sequences with high accuracy by integrating de novo sequencing peptides, intensity, and positional confidence scores from database and homology searches.
Antibody mimetic. Antibody mimetics are organic compounds, like antibodies, that can specifically bind antigens. They consist of artificial peptides or proteins, or aptamer-based nucleic acid molecules with a molar mass of about 3 to 20 kDa. Antibody fragments, such as Fab and nanobodies are not considered as antibody mimetics. Common advantages over antibodies are better solubility, tissue penetration, stability towards heat and enzymes, and comparatively low production costs. Antibody mimetics have been developed and commercialized as research, diagnostic and therapeutic agents. Binding antibody unit. BAU (binding antibody unit, often as BAU/mL) is a measurement unit defined by the WHO for the comparison of assays detecting the same class of immunoglobulins with the same specificity.
Alessandro Scarlatti Pietro Alessandro Gaspare Scarlatti (2 May 1660 – 22 October 1725) was an Italian Baroque composer, known especially for his operas and chamber cantatas. He is considered the most important representative of the Neapolitan school of opera. Nicknamed by his contemporaries "the Italian Orpheus", he divided his career between Naples and Rome, where he received his training; a significant part of his works was composed for the papal city. He is often considered the founder of the Neapolitan school, although he has only been its most illustrious representative: his contribution, his originality and his influence were essential, as well as lasting, both in Italy and in Europe. Particularly known for his operas, he brought the Italian dramatic tradition to its maximum development, begun by Monteverdi at the beginning of 17th century and continued by Cesti, Cavalli, Carissimi, Legrenzi and Stradella, designing the final form of the "Da capo aria", imitated throughout Europe. He was also the inventor of the Italian overture in three movements (which was of the highest importance in the development of the symphony), of the four-part sonata (progenitor of the modern string quartet), and of the technique of motivic development. He was a model for the musical theater of his time, as evoked by Händel's Italian works, deeply influenced by his theatrical music. Eclectic, Scarlatti also worked on all the other common genres of his time, from the sonata to the concerto grosso, from the motet to the mass, from the oratorio to the cantata, the latter being a genre in which he was an undisputed master.
He was the father of two other composers, Domenico Scarlatti and Pietro Filippo Scarlatti. Life. Scarlatti was born in Palermo (or in Trapani), then part of the Kingdom of Sicily. He is generally said to have been a pupil of Giacomo Carissimi in Rome, and some theorize that he had some connection with northern Italy because his early works seem to show the influence of Stradella and Legrenzi. The production at Rome of his opera "Gli equivoci nel sembiante" (1679) gained him the support of Queen Christina of Sweden (who at the time was living in Rome), and he became her "maestro di cappella". In February 1684 he became "maestro di cappella" to the viceroy of Naples, perhaps through the influence of his sister, an opera singer, who might have been the mistress of an influential Neapolitan noble. Here he produced a long series of operas, remarkable chiefly for their fluency and expressiveness, as well as other music for state occasions. In 1702 Scarlatti left Naples and did not return until the Spanish domination had been superseded by that of the Austrians. In the interval he enjoyed the patronage of Ferdinando de' Medici, for whose private theatre near Florence he composed operas, and of Cardinal Ottoboni, who made him his "maestro di cappella", and procured him a similar post at the Basilica di Santa Maria Maggiore in Rome in 1703.
After visiting Venice and Urbino in 1707, Scarlatti took up his duties in Naples again in 1708, and remained there until 1717. By this time Naples seems to have become tired of his music; the Romans, however, appreciated it better, and it was at the Teatro Capranica in Rome that he produced some of his finest operas ("Telemaco", 1718; "Marco Attilio Regolò", 1719; "La Griselda", 1721), as well as some noble specimens of church music, including a "Messa di Santa Cecilia" for chorus and orchestra, composed in honor of Saint Cecilia for Cardinal Francesco Acquaviva in 1721. His last work on a large scale appears to have been the unfinished "Erminia" serenata for the marriage of the prince of Stigliano in 1723. He died in Naples in 1725 and is entombed there at the church of Santa Maria di Montesanto. Music. Scarlatti's music forms an important link between the early Baroque Italian vocal styles of the 17th century, with their centers in Florence, Venice and Rome, and the classical school of the 18th century. Scarlatti's style, however, is more than a transitional element in Western music; like most of his Naples colleagues he shows an almost modern understanding of the psychology of modulation and also frequently makes use of the ever-changing phrase lengths so typical of the Napoli school.
His early operas—"Gli equivoci nel sembiante" 1679; "L'honestà negli amori" 1680, containing the famous aria "Già il sole dal Gange"; "Il Pompeo" 1683, containing the well-known airs "O cessate di piagarmi" and "Toglietemi la vita ancor," and others down to about 1685—retain the older cadences in their recitatives, and a considerable variety of neatly constructed forms in their charming little arias, accompanied sometimes by the string quartet, treated with careful elaboration, sometimes with the continuo alone. By 1686, he had definitely established the "Italian overture" form (second edition of "Dal male il bene"), and had abandoned the ground bass and the binary form air in two stanzas in favour of the ternary form or da capo type of air. His best operas of this period are "La Rosaura" (1690, printed by the Gesellschaft für Musikforschung), and "Pirro e Demetrio" (1694), in which occur the arias "Le Violette", and "Ben ti sta, traditor". From about 1697 onwards ("La caduta del Decemviri"), influenced partly perhaps by the style of Giovanni Bononcini and probably more by the taste of the viceregal court, his opera arias become more conventional and commonplace in rhythm, while his scoring is hasty and crude, yet not without brilliance ("L'Eraclea", 1700), the oboes and trumpets being frequently used, and the violins often playing in unison. The operas composed for Ferdinando de' Medici are lost; they might have given a more favourable idea of his style as his correspondence with the prince shows that they were composed with a very sincere sense of inspiration.
"Mitridate Eupatore", accounted his masterpiece, composed for Venice in 1707, contains music far in advance of anything that Scarlatti had written for Naples, both in technique and in intellectual power. The later Neapolitan operas ("L'amor volubile e tiranno" 1709; "La principessa fedele" 1710; "Tigrane", 1714, &c.) are showy and effective rather than profoundly emotional; the instrumentation marks a great advance on previous work, since the main duty of accompanying the voice is thrown upon the string quartet, the harpsichord being reserved exclusively for the noisy instrumental "ritornelli". In his opera "Teodora" (1697) he originated the use of the orchestral "ritornello". His last group of operas, composed for Rome, exhibit a deeper poetic feeling, a broad and dignified style of melody, a strong dramatic sense, especially in accompanied recitatives, a device which he himself had been the first to use as early as 1686 ("Olimpia vendicata") and a much more modern style of orchestration, the horns appearing for the first time, and being treated with striking effect.
Besides the operas, oratorios ("Agar et Ismaele esiliati", 1684; "La Maddalena", 1685; "La Giuditta", 1693; "Humanita e Lucifero", 1704; "Christmas Oratorio", c. 1705; "Cain", 1707; "S. Filippo Neri", 1714; and others) and serenatas, which all exhibit a similar style, Scarlatti composed upwards of five hundred chamber-cantatas for solo voice. These represent the most intellectual type of chamber-music of their period, and it is to be regretted that they have remained almost entirely in manuscript, since a careful study of them is indispensable to anyone who wishes to form an adequate idea of Scarlatti's development. His few remaining Masses (the story of his having composed two hundred is hardly credible) and church music in general are comparatively unimportant, except the great "Saint Cecilia Mass" (1721), which is one of the first attempts at the style which reached its height in the great Masses of Johann Sebastian Bach and Ludwig van Beethoven. His instrumental music, though not without interest, is curiously antiquated as compared with his vocal works.
Aston Martin Aston Martin Lagonda Global Holdings PLC () is a British manufacturer of luxury sports cars and grand tourers. Its predecessor was founded in 1913 by Lionel Martin and Robert Bamford. Headed from 1947 by David Brown, it became associated with expensive grand touring cars in the 1950s and 1960s, and with the fictional character James Bond following his use of a DB5 model in the 1964 film "Goldfinger". Their grand tourers and sports cars are regarded as a British cultural icon. Aston Martin has held a royal warrant as purveyor of motorcars to Charles III (as Prince of Wales and later as King) since 1982, and has over 160 car dealerships in 53 countries, making it a global automobile brand. The company is traded on the London Stock Exchange and is a constituent of the FTSE 250 Index. In 2003 it received the Queen's Award for Enterprise for outstanding contribution to international trade. The company has survived seven bankruptcies throughout its history. The headquarters and main production of its sports cars and grand tourers are in a facility in Gaydon, Warwickshire, England, on the former site of RAF Gaydon, adjacent to the Jaguar Land Rover Gaydon Centre. The old facility in Newport Pagnell, Buckinghamshire, is the present home of the Aston Martin Works classic car department, which focuses on heritage sales, service, spares and restoration operations. The factory in St Athan, Wales, features three converted 'super-hangars' from MOD St Athan, and serves as the production site of Aston Martin's SUV, the DBX.
Aston Martin has been involved in motorsport at various points in its history, mainly in sports car racing, and also in Formula One. The Aston Martin brand is increasingly being used, mostly through licensing, on other products including a submarine, real estate development, and aircraft. History. Founding. Aston Martin was founded in 1913 by Lionel Martin and Robert Bamford. The two had joined forces as Bamford & Martin the previous year to sell cars made by Singer from premises in Callow Street, London where they also serviced GWK and Calthorpe vehicles. Martin raced specials at Aston Hill near Aston Clinton, and the pair decided to make their own vehicles. The first car to be named "Aston Martin" was created by Martin by fitting a four-cylinder Coventry-Simplex engine to the chassis of a 1908 Isotta Fraschini. They acquired premises at Henniker Mews in Kensington and produced their first car in March 1915. Production could not start because of the outbreak of the First World War, when Martin joined the Admiralty and Bamford joined the Army Service Corps. 1918–1939: Interwar years.
After the war they found new premises at Abingdon Road, Kensington and designed a new car. Bamford left in 1920 and Bamford & Martin was revitalised with funding from Louis Zborowski. In 1922, Bamford & Martin produced cars to compete in the French Grand Prix, which went on to set world speed and endurance records at Brooklands. Three works Team Cars with 16-valve twin cam engines were built for racing and record-breaking: chassis number 1914, later developed as the Green Pea; chassis number 1915, the Razor Blade record car; and chassis number 1916, later developed as the Halford Special. Approximately 55 cars were built for sale in two configurations; long chassis and short chassis. Bamford & Martin went bankrupt in 1924 and was bought by Dorothea, Lady Charnwood, who put her son John Benson on the board. Bamford & Martin got into financial difficulty again in 1925 and Martin was forced to sell the company (Bamford had already left it in 1920). Later that year, Bill Renwick, Augustus (Bert) Bertelli and investors including Lady Charnwood took control of the business. They renamed it Aston Martin Motors and moved it to the former Whitehead Aircraft Limited Hanworth works in Feltham. Renwick and Bertelli had been in partnership some years and had developed an overhead-cam four-cylinder engine using Renwick's patented combustion chamber design, which they had tested in an Enfield-Allday chassis. The only "Renwick and Bertelli" motor car made, it was known as "Buzzbox" and still survives.
The pair had planned to sell their engine to motor manufacturers, but having heard that Aston Martin was no longer in production realised they could capitalise on its reputation to jump-start the production of a completely new car. Between 1926 and 1937 Bertelli was both technical director and designer of all new Aston Martins, since known as "Bertelli cars". They included the 1½-litre "T-type", "International", "Le Mans", "MKII" and its racing derivative, the "Ulster", and the 2-litre 15/98 and its racing derivative, the "Speed Model". Most were open two-seater sports cars bodied by Bert Bertelli's brother , with a small number of long-chassis four-seater tourers, dropheads and saloons also produced. Bertelli was a competent driver keen to race his cars, one of few owner/manufacturer/drivers. The "LM" team cars were very successful in national and international motor racing including at Le Mans. Financial problems reappeared in 1932. Aston Martin was rescued for a year by Lance Prideaux Brune before passing it on to Sir Arthur Sutherland. In 1936, Aston Martin decided to concentrate on road cars, producing just 700 until World War II halted work. Production shifted to aircraft components during the war. 1947–1972: David Brown.
In 1947, old-established (1860) privately owned Huddersfield gear and machine tools manufacturer David Brown Limited bought Aston Martin, putting it under control of its Tractor Group. David Brown became Aston Martin's latest saviour. He also acquired Lagonda, without its factory, for its 2.6-litre W. O. Bentley-designed engine. Lagonda moved operations to Newport Pagnell and shared engines, resources and workshops. Aston Martin began to build the classic "DB" series of cars. In April 1950, they announced planned production of their Le Mans prototype to be called the DB2, followed by the DB2/4 in 1953, the DB2/4 MkII in 1955, the DB Mark III in 1957 and the Italian-styled 3.7 L DB4 in 1958. While these models helped Aston Martin establish a good racing pedigree, the DB4 stood out and yielded the famous DB5 in 1963. Aston stayed true to its grand touring style with the DB6 (1965–70), and DBS (1967–1972). The six-cylinder engines of these cars from 1954 up to 1965 were designed by Tadek Marek. 1972–1975: William Willson.
Aston Martin was often financially troubled. In 1972, David Brown paid off all its debts, said to be £5 million or more, and handed it for £101 to Company Developments, a Birmingham-based investment bank consortium chaired by accountant William Willson. More detail on this period may be read at Willson's biography. The worldwide recession, lack of working capital and the difficulties of developing an engine to meet California's exhaust emission requirements – it stopped the company's US sales – again pulled Aston Martin into receivership at the end of 1974. The company had employed 460 workers when the manufacturing plant closed. 1975–1981: Sprague and Curtis. The receiver sold the business in April 1975 for £1.05 million to North American businessman Peter Sprague of National Semiconductor, Toronto hotelier George Minden, and Jeremy Turner, a London businessman, who insisted to reporters that Aston Martin remained a British controlled business. Sprague later claimed he had fallen in love with the factory, not the cars, the workforce's craftsmanship dedication and intelligence. At this point, he and Minden had brought in investor Alan Curtis, a British office property developer, together with George Flather, a retired Sheffield steel magnate.
Six months later, in September 1975, the factory – shut down the previous December – re-opened under its new owner as Aston Martin Lagonda Limited with 100 employees, and planned to lift staff to 250 by the end of 1975. In January 1976, AML revealed that it now held orders for 150 cars for the US, 100 for other markets and another 80 from a Japanese importing agency. At the Geneva Motor Show, Fred Hartley, managing director and sales director for 13 years before that, announced he had resigned over "differences in marketing policy". The new owners pushed Aston Martin into modernising its line, introducing the V8 Vantage in 1977, the convertible Volante in 1978, and the one-off Bulldog styled by William Towns in 1980. Towns also styled the futuristic new Lagonda saloon, based on the V8 model. Curtis, who had a 42% stake in Aston Martin, also brought about a change in direction from the usual customers who were Aston Martin fans, to successful young married businessmen. Prices had been increased by 25%. There was speculation that AML was about to buy Italian automobile manufacturer Lamborghini. At the end of the 1970s, there was widespread debate about running MG into the Aston Martin consortium. 85 Conservative MPs formed themselves into a pressure group to get British Leyland to release their grip and hand it over. CH Industrials plc (car components) bought a 10% share in AML. But in July 1980, blaming a recession, AML cut back their workforce of 450 by more than 20%, making those people redundant. 1981–1987: Victor Gauntlett.
In January 1981, there having been no satisfactory revival partners, Alan Curtis and Peter Sprague announced they had never intended to maintain a long-term financial stake in Aston Martin Lagonda and it was to be sold to Pace Petroleum's Victor Gauntlett. Sprague and Curtis pointed out that under their ownership AML finances had improved to where an offer for MG might have been feasible. Gauntlett bought a 12.5% stake in Aston Martin for £500,000 via Pace Petroleum in 1980, with Tim Hearley of CH Industrials taking a similar share. Pace and CHI took over as joint 50/50 owners at the beginning of 1981, with Gauntlett as executive chairman. Gauntlett also led the sales team, and after some development and publicity when the Lagonda became the world's fastest four-seater production car, was able to sell the car in Oman, Kuwait, and Qatar. In 1982, Aston Martin was granted a Royal Warrant of Appointment by the Prince of Wales. Understanding that it would take some time to develop new Aston Martin products, they created an engineering service subsidiary to develop automotive products for other companies. It was decided to use a trade name of Salmons & Son, their in-house coachbuilder, Tickford, which Aston Martin had bought in 1955. Tickford's name had been long associated with expensive high-quality carriages and cars along with their folding roofs. New products included a Tickford Austin Metro, a Tickford Ford Capri and even Tickford train interiors, particularly on the Jaguar XJS. Pace continued sponsoring racing events, and now sponsored all Aston Martin Owners Club events, taking a Tickford-engined Nimrod Group C car owned by AMOC President Viscount Downe, which came third in the Manufacturers Championship in both 1982 and 1983. It also finished seventh in the 1982 24 Hours of Le Mans race. However, sales of production cars were now at an all-time low of 30 cars produced in 1982.
As trading became tighter in the petroleum market, and Aston Martin was requiring more time and money, Gauntlett agreed to sell Hays/Pace to the Kuwait Investment Office in September 1983. As Aston Martin required greater investment, he also agreed to sell his share holding to American importer and Greek shipping tycoon Peter Livanos, who invested via his joint venture with Nick and John Papanicolaou, ALL Inc. Gauntlett remained chairman of AML, 55% of the stake was owned by ALL, with Tickford a 50/50 venture between ALL and CHI. The uneasy relationship was ended when ALL exercised options to buy a larger share in AML; CHI's residual shares were exchanged for CHI's complete ownership of Tickford, which retained the development of existing Aston Martin projects. In 1984, Papanicolaou's Titan shipping business was in trouble so Livanos's father George bought out the Papanicolaou's shares in ALL, while Gauntlett again became a shareholder with a 25% holding in AML. The deal valued Aston Martin/AML at £2 million, the year it built its 10,000th car.
Although as a result Aston Martin had to make 60 members of the workforce redundant, Gauntlett bought a stake in Italian styling house Zagato, and resurrected its collaboration with Aston Martin. In 1986, Gauntlett negotiated the return of the fictional British secret agent James Bond to Aston Martin. Cubby Broccoli had chosen to recast the character using actor Timothy Dalton, in an attempt to re-root the Bond-brand back to a more Sean Connery-like feel. Gauntlett supplied his personal pre-production Vantage for use in the filming of "The Living Daylights", and sold a Volante to Broccoli for use at his home in America. Gauntlett turned down the role of a KGB colonel in the film, however: "I would have loved to have done it but really could not afford the time." 1987–2007: Ford Motor Company. As Aston Martin needed funds to survive in the long term, Ford bought a 75% stake in the company in 1987, and bought the rest later. In May of that year, Victor Gauntlett and Prince Michael of Kent were staying at the home of Contessa Maggi, the wife of the founder of the original Mille Miglia, while watching the revival event. Another house guest was Walter Hayes, vice-president of Ford of Europe. Despite problems over the previous acquisition of AC Cars, Hayes saw the potential of the brand and the discussion resulted in Ford taking a share holding in September 1987. In 1988, having produced some 5,000 cars in 20 years, a revived economy and successful sales of limited edition Vantage, and 52 Volante Zagato coupés at £86,000 each; Aston Martin finally retired the ancient V8 and introduced the Virage range.
Although Gauntlett was contractually to stay as chairman for two years, his racing interests took the company back into sports car racing in 1989 with limited European success. However, with engine rule changes for the 1990 season and the launch of the new Volante model, Ford provided the limited supply of Cosworth engines to the Jaguar cars racing team. As the entry-level DB7 would require a large engineering input, Ford agreed to take full control of Aston Martin, and Gauntlett handed over Aston Martin's chairmanship to Hayes in 1991. In 1992, the high-performance variant of the Virage called the Vantage was announced, and the following year Aston Martin renewed the DB range by announcing the DB7. By 1993, Ford had fully acquired the company after having built a stake in 1987. Ford placed Aston Martin in the Premier Automotive Group, invested in new manufacturing and ramped up production. In 1994, Ford opened a new factory at Banbury Road in Bloxham to manufacture the DB7. In 1995, Aston Martin produced a record 700 cars. Until the Ford era, cars had been produced by hand coachbuilding craft methods, such as the English wheel. During the mid-1990s, the Special Projects Group, a secretive unit with Works Service at Newport Pagnell, created an array of special coach-built vehicles for the Brunei royal family. In 1998, the 2,000th DB7 was built, and in 2002, the 6,000th, exceeding production of all of the previous DB series models. The DB7 range was revamped by the addition of more powerful V12 Vantage models in 1999, and in 2001, Aston Martin introduced the V12-engined flagship model called the Vanquish which succeeded the aging Virage (now called the V8 Coupé).
At the North American International Auto Show in Detroit, Michigan in 2003, Aston Martin introduced the V8 Vantage concept car. Expected to have few changes before its introduction in 2005, the Vantage brought back the classic V8 engine to allow Aston Martin to compete in a larger market. 2003 also saw the opening of the Gaydon factory, the first purpose-built factory in Aston Martin's history. The facility is situated on a site of a former RAF V Bomber airbase, with an front building for offices, meeting rooms and customer reception, and a production building. Also introduced in 2003 was the DB9 coupé, which replaced the ten-year-old DB7. A convertible version of the DB9, the DB9 Volante, was introduced at the 2004 Detroit auto show. In October 2004, Aston Martin set up the dedicated Aston Martin Engine Plant (AMEP) within the Ford Germany plant in Niehl, Cologne. With the capacity to produce up to 5,000 engines a year by 100 specially trained personnel, like traditional Aston Martin engine production from Newport Pagnell, assembly of each unit was entrusted to a single technician from a pool of 30, with V8 and V12 variants assembled in under 20 hours. By bringing engine production back to within Aston Martin, the promise was that Aston Martin would be able to produce small runs of higher performance variants' engines. This expanded engine capacity allowed the entry-level V8 Vantage sports car to enter production at the Gaydon factory in 2006, joining the DB9 and DB9 Volante.
In December 2003, Aston Martin announced it would return to motor racing in 2005. A new division was created, called Aston Martin Racing, which became responsible, together with Prodrive, for the design, development, and management of the DBR9 program. The DBR9 competes in the GT class in sports car races, including the world-famous 24 Hours of Le Mans. In 2006, an internal audit led Ford to consider divesting itself of parts of its Premier Automotive Group. After suggestions of selling Jaguar Cars, Land Rover, or Volvo Cars were weighed, Ford announced in August 2006 it had engaged UBS AG to sell all or part of Aston Martin at auction. 2007–2018: Private Limited Company. On 12 March 2007, a consortium led by Prodrive chairman David Richards purchased Aston Martin for £475 million (US$848 million). The group included American investment banker John Sinders and two Kuwaiti companies namely Investment Dar and Adeem Investment. Prodrive had no financial involvement in the deal. Ford kept a stake in Aston Martin valued at £40 million (US$70 million).
To demonstrate the V8 Vantage's durability across hazardous terrain and promote the car in China, the first east–west crossing of the Asian Highway was undertaken between June and August 2007. A pair of Britons drove from Tokyo to Istanbul before joining the European motorway network for another to London. The promotion was so successful Aston Martin opened dealerships in Shanghai and Beijing within three months. On 19 July 2007, the Newport Pagnell plant rolled out the last of nearly 13,000 cars made there since 1955, a Vanquish S. The Tickford Street facility was converted and became the home of the Aston Martin Works classic car department which focuses on heritage sales, service, spares and restoration operations. UK production was subsequently concentrated on the facility in Gaydon on the former RAF V Bomber airbase. In March 2008, Aston Martin announced a partnership with Magna Steyr to outsource manufacture of over 2,000 cars annually to Graz, Austria, reassuringly stating: "The continuing growth and success of Aston Martin is based upon Gaydon as the focal point and heart of the business, with the design and engineering of all Aston Martin products continuing to be carried out there."
More dealers in Europe and the new pair in China brought the total to 120 in 28 countries. On 1 September 2008, Aston Martin announced the revival of the Lagonda marque, proposing a concept car to be shown in 2009 to coincide with the brand's 100th anniversary. The first production cars were slated for production in 2012. In December 2008, Aston Martin announced it would cut its workforce from 1,850 to 1,250 due to the economic recession. The first four-door Rapide grand tourers rolled out of the Magna Steyr factory in Graz, Austria, in 2010. The contract manufacturer provides dedicated facilities to ensure compliance with the exacting standards of Aston Martin and other marques, including Mercedes-Benz. Then CEO of the company, Ulrich Bez had publicly speculated about outsourcing all of Aston Martin's operations with the exception of marketing. In September 2011, it was announced that production of the Rapide would be returned to Gaydon in the second half of 2012, restoring all of the company's automobile manufacture there.
Italian private equity fund Investindustrial signed a deal on 6 December 2012 to buy a 37.5% stake in Aston Martin, investing £150 million as a capital increase. This was confirmed by Aston Martin in a press release on 7 December 2012. David Richards left Aston Martin in 2013, returning to concentrate on Prodrive. In April 2013, it was reported that Bez would be leaving his role as the chief executive officer to take up a more ambassadorial position. On 2 September 2014, Aston Martin announced it had appointed the Nissan executive Andy Palmer as the new CEO with Bez retaining a position as non-executive chairman. As sales had been declining from 2015, Aston Martin sought new customers (particularly wealthy female buyers) with introducing concept cars like the DBX SUV along with track focused cars like the Vulcan. According to Palmer, the troubles started when sales of the DB9 failed to generate sufficient fund to develop next-generation models which led to a downward spiral of declining sales and profitability.
Palmer outlined that the company plans to develop two new platforms, add a crossover, refresh its supercar lineup and leverage its technology alliance with Daimler as part of its six-year plan to make the 100-year-old British brand consistently profitable. He stated, "In the first century we went bankrupt seven times. The second century is about making sure that is not the case." In preparation for its next-generation of sports cars, the company invested £20 million ($33.4 million) to expand its manufacturing plant in Gaydon. The expansion at the Gaydon plant includes a new chassis and pilot build facility, as well as an extension of the parts and logistics storage area, and new offices. In total, Aston Martin will add approximately to the plant. In 2014, Aston Martin suffered a pre-tax loss of £72 million, almost triple of the amount of 2013 selling 3,500 cars during the year, well below the 7,300 cars sold in 2007 and 4,200 sold in 2013 respectively. In March 2014, Aston Martin issued "payment in kind" notes of US$165 million, at 10.25% interest, in addition to the £304 million of senior secured notes at 9.25% issued in 2011. Aston Martin also had to secure an additional investment of £200 million from its shareholders to fund development of new models. It was reported that Aston Martin's pre-tax losses for 2016 increased by 27% to £162.8 million, the sixth year it continued to suffer a loss.
In February 2016, the company selected a site in St Athan, South Wales for its new factory. The Welsh facility was unanimously chosen by Aston's board despite fierce competition from other locations as far afield as the Americas, Europe, the Middle East, as well as other sites in the UK believed to be Bridgend, Birmingham, and Coventry. The facility featured three existing 'super-hangars' of MOD St Athan. Construction work of converting the hangars commenced in April 2017. Aston Martin returned to profit in 2017 after selling over 5,000 cars. The company made a pre-tax profit of £87 million compared with a £163 million loss in 2016. 2017 also marked the return of production of the Newport Pagnell facility ten years after it originally ceased. 2013–present: Partnership with Mercedes-Benz Group. In December 2013, Aston Martin signed a deal with Mercedes-Benz Group (at the time known as Daimler) to supply the next generation of Aston Martin cars with Mercedes-AMG engines. Mercedes-AMG also was to supply Aston Martin with electrical systems. This technical partnership was intended to support Aston Martin's launch of a new generation of models that would incorporate new technology and engines. In exchange, Mercedes will get as much as 5% equity in Aston Martin and a non-voting seat on its board. The first model to sport the Mercedes-Benz technology was the DB11, announced at the 86th Geneva Motor Show in March 2016. It featured Mercedes-Benz electronics for the entertainment, navigation and other systems. It was also the first model to use Mercedes-AMG V8 engines. In October 2020, Mercedes confirmed it will increase its holding "in stages" from 5% to 20%. In return, Aston Martin will have access to Mercedes-Benz hybrid and electric drivetrain technologies for its future models. 2018–present: Listed on the London Stock Exchange.
After "completing a turnaround for the once perennially loss-making company that could now be valued at up to 5 billion pounds ($6.4 billion)," and now reporting a full-year pre-tax profit of £87 million (compared with a £163 million loss in 2016) Aston Martin in August 2018 announced plans to float the company at the London Stock Exchange as Aston Martin Lagonda Global Holdings plc. The company was the subject of an initial public offering on the London Stock Exchange on 3 October 2018. In the same year, Aston Martin opened a new vehicle dynamics test and development centre at Silverstone's Stowe Circuit alongside a new HQ in London. In June 2019, the company opened its new factory in St Athan for the production of its first-ever SUV the DBX. The factory was finally completed and officially opened on 6 December 2019. When full production begins in the second quarter of 2020, around 600 people will be employed at the factory, rising to 750 when peak production is reached. On 31 January 2020 it was announced that Canadian billionaire and investor Lawrence Stroll was leading a consortium, Yew Tree Overseas Limited, who will pay £182 million in return for 16.7% stake in the company. The re-structuring includes a £318 million cash infusion through a new rights issue, generating a total of £500 million for the company. Stroll will also be named as chairman, replacing Penny Hughes. Swiss pharmaceutical magnate Ernesto Bertarelli and Mercedes-AMG Petronas F1 team principal and CEO Toto Wolff have also joined the consortium, acquiring 3.4% and 4.8% stakes, respectively. In March 2020, Stroll increased his stake in the company to 25%.
On 26 May 2020, Aston Martin announced that Andy Palmer had stepped down as CEO. Tobias Moers of Mercedes-AMG will succeed him starting 1 August, with Keith Stanton as interim chief operating officer. In June 2020, the company announced that it cut out 500 jobs as a result of the poor sales, an outcome of the COVID-19 pandemic lockdown. In March 2021, executive chairman Lawrence Stroll stated that the company plans on building electric vehicles by 2025. In May 2022, Aston Martin named 76-year-old Amedeo Felisa as the new chief executive officer, replacing Tobias Moers. Roberto Fedeli was also announced as the new chief technical officer. In late 2020, Aston Martin was involved in a controversy in which it was accused of using a report to spread disputed information about electric vehicles in the wake of the UK's declaration to end the sale of combustion engine vehicles by 2030, with some in the media dubbing the controversy as "Astongate". In November 2020, a communications agency called Clarendon Communications published a report comparing the environmental impact of various powertrain options for cars. After the report received coverage from "The Sunday Times" and other publications, it emerged that the company had been set up in February that year and was registered under the name of Rebecca Stephens – the wife of James Stephens, who is the government affairs director of Aston Martin Lagonda. Citing a study by Polestar, the report stated that electric vehicles would need to be driven before they would have lower overall emissions than a petrol car. This statement was disputed by electric vehicle researcher Auke Hoekstra, who argued that the report underestimated the emissions from combustion engine vehicles and did not consider the emissions from creating petrol. According to him, a typical EV would need to drive 16,000–18,000 miles (25,700–30,000 km) to offset the emissions from manufacture. Bosch and a number of other companies were also involved with the report.
In July 2022, Saudi Arabia's Public Investment Fund (PIF) will take a stake in the company through a £78 million equity placing as well as a £575 million separate rights issue, giving it two board seats in the company. After the rights issue, the Saudi fund will have a 16.7% stake in Aston Martin, behind the 18.3% holding by Stroll's Yew Tree consortium while the Mercedes-Benz Group will own 9.7%. In September 2022, Chinese automaker Geely acquired a 7.6% stake in the company. In December 2022, Stroll and the Yew Tree consortium increased their stake in the company to 28.29%. In May 2023, Geely increased its stake to 17%, becoming the third-largest shareholder after the Yew Tree consortium and the Saudi Arabia Public Investment Fund. In June 2023, Aston Martin signed an agreement with Lucid Motors after selecting it to help supply electric motors, powertrains, and battery systems for its upcoming range of fully electric cars. In return, Aston Martin will make cash payments and issue a 3.7percent stake in its company to Lucid, worth $232million in total. In September 2023, the Yew Tree consortium increased their stake by 3.27% to 26.23%. In October 2023, Aston Martin announced that it would compete in the FIA World Endurance Championship and IMSA SportsCar Championship in 2025. In April 2024, the company said it would push back production of its first electric vehicle to 2027. In March 2024, Aston Martin announced Adrian Hallmark as its new CEO beginning 1 October 2024, replacing Amedeo Felisa.
In September 2024, Aston Martin issued a profit warning, saying it had been hit by a fall in demand in China. In November 2024, Aston Martin issued another warning following a minor delay in the deliveries of their Valiant model. In response, they said they would issue new shares and debt totalling £210 million. In February 2025, CEO Adrian Hallmark announced the company would again push back production of its first electric vehicle to 2030. On 31 March 2025, the Yew Tree Consortium is set to inject an additional £52.5 million into the marque by purchasing 75 million shares at 70 pence per share, increasing its stake to 33%. The company will also sell shares in the Formula One racing team that it sponsors. In total, the two transactions will raise £125 million. Sales at auction. In August 2017, a 1956 Aston Martin DBR1/1 sold at a Sotheby's auction at the Pebble Beach, California Concours d'Elegance for US$22,550,000, which made it the most expensive British car ever sold at an auction, according to Sotheby's. The car had previously been driven by Carroll Shelby and Stirling Moss.
In 2015 a 1962 Aston Martin DB4 GT Zagato for US$14,300,000 in New York, and a 1963 Aston Martin DP215 for US$21,455,000 in August 2018. Brand expansion. Since 2015, Aston Martin has sought to increase its appeal to women as a luxury lifestyle brand. A female advisory panel was established to adapt the design of the cars to the taste of women. In September 2016, a 37-foot-long Aston Martin speedboat was unveiled called the Aston Martin AM37 powerboat. In May 2018, Aston Martin launched a submersible called Project Neptune in partnership with submarine building company Triton Submarines. Aston Martin has collaborated with the luxury clothing company Hackett London to deliver items of clothing. In November 2017, Aston Martin unveiled a special limited edition bicycle after collaborating with bicycle manufacturer Storck. Aston Martin and global property developer G&G Business Developments constructed a 66-storey luxury condominium tower called Aston Martin Residences at 300 Biscayne Boulevard Way in Miami, Florida, which opened in 2024.
In July 2018, Aston Martin unveiled the Volante Vision Concept, a luxury concept aircraft with vertical take-off and landing capabilities. Also in July, a Lego version of James Bond's DB5 car was put on sale, and an Aston Martin-branded watch was released in collaboration with TAG Heuer. In October 2018, Aston Martin announced it was opening a design and brand studio in Shanghai. Motorsport. Aston Martin is currently associated with two different racing organisations. The Aston Martin Formula One team which competes in the Formula One Championship and Aston Martin Racing which currently competes in the FIA World Endurance Championship. Both racing organisations use the Aston Martin brand, but are not directly owned by Aston Martin. The Aston Martin Formula One team is owned by major Aston Martin shareholder Lawrence Stroll and operated by his company AMR GP, while Aston Martin Racing is operated by racing company Prodrive as part of an agreement with Aston Martin. Formula One. Aston Martin participated as a Formula One constructor in and entering six races over the two years but failing to score any points. In January 2020, it was announced that the Racing Point F1 Team is due to be rebranded as Aston Martin for the 2021 season, as a result of a funding investment led by Racing Point owner Lawrence Stroll. As part of the rebrand, the team switched their racing colour of BWT pink to a modern iteration of Aston Martin's British racing green. The Aston Martin AMR21 was unveiled in March 2021 and became Aston Martin's first Formula One car after a 61-year absence from the sport. Sponsorships. Aston Martin sponsors 2. Bundesliga club 1860 Munich.
Albert Pike Albert Pike (December 29, 1809April 2, 1891) was an American author, poet, orator, editor, lawyer, jurist and Confederate States Army general who served as an associate justice of the Arkansas Supreme Court in exile from 1864 to 1865. He had previously served as a senior officer of the Confederate States Army, commanding the District of Indian Territory in the Trans-Mississippi Theater. A prominent member of the Freemasons, Pike served as the Sovereign Grand Commander of the Supreme Council, Scottish Rite, Southern Jurisdiction from 1859 to 1891. Early life and education. Albert Pike was born in Boston, Massachusetts, on December 29, 1809, the son of Benjamin and Sarah (Andrews) Pike. He grew up in Byfield and Newburyport, Massachusetts. His colonial ancestors had settled in the area in 1635, and included John Pike (1613–1688/1689), the founder of Woodbridge, New Jersey. He attended school in Newburyport and Framingham until he was 15. In August 1825, he passed entrance exams at Harvard University, though when the college requested payment of tuition fees for the first two years, he chose not to attend. He began a program of self-education, later teaching school in Gloucester, North Bedford, Fairhaven and Newburyport.
Pike was an imposing figure; tall and with hair that reached his shoulders and a long beard. In 1831, he left Massachusetts to travel west, first stopping in Nashville, Tennessee. He later moved to St. Louis, Missouri, where he joined a hunting and trading expedition to Taos, New Mexico. En route his horse broke and ran, forcing Pike to walk the remaining to Taos. After this, he joined a trapping expedition to the Llano Estacado in New Mexico and Texas. Trapping was minimal and, after traveling about , half of it on foot, he finally arrived at Fort Smith, Arkansas. Career. Settling in Arkansas in 1833, Pike taught in a school and wrote a series of articles for the Little Rock "Arkansas Advocate" under the pen name of "Casca." The articles were sufficiently well received for him to be asked to join the newspaper's staff. Under Pike's administration, the "Advocate" promoted the viewpoint of the Whig Party in a politically volatile and divided Arkansas in December 1832. After marrying Mary Ann Hamilton in 1834, he purchased the newspaper.
He was the first reporter for the Arkansas Supreme Court. He wrote a book (published anonymously), titled "The Arkansas Form Book", which was a guidebook for lawyers. Pike began to study law and was admitted to the bar in 1837, selling the "Advocate" the same year. (At least one source indicates that Pike read Kent and Blackstone and was admitted to the bar in 1834 by Superior Court judge Thomas J. Lacy, after a perfunctory examination.) He proved to be a highly effective lawyer, representing clients in courts at every level. This continued after he received permission in 1849 to practice before the United States Supreme Court. He also made several contacts among the Native American tribes in the area. He specialized in claims on behalf of Native Americans against the federal government. In 1852, he represented the Creek Nation before the Supreme Court in a claim regarding ceded tribal land. In 1854 he advocated for the Choctaw and Chickasaw, although compensation later awarded to the tribes in 1856 and 1857 was insufficient. These relationships were to influence the course of his Civil War service.
Pike also began a campaign of newspaper essays urging support for the construction of a transcontinental railroad to extend from New Orleans to the Pacific coast. He moved to New Orleans in 1853 and prepared to pass the state bar in furtherance of his campaign. He ultimately secured a charter from the Louisiana State Legislature for a project, following which he returned to Little Rock in 1857. He joined the anti-Catholic Know Nothing Party at its founding; in the summer of 1854, he helped introduce the party in Arkansas. He attended the national convention in 1856, but walked out when it failed to adopt a pro-slavery platform. Additionally, Pike wrote on several legal subjects. He also continued writing poetry, a hobby he had begun in his youth in Massachusetts. His poems were highly regarded in his day, but are now mostly forgotten. Several volumes of his works were privately published posthumously by his daughter. In 1859, he received an honorary Master of Arts degree from Harvard. Poetry. As a young man of letters, Pike wrote poetry, and he continued to do so for the rest of his life. At 23, he published his first poem, "Hymns to the Gods." Later work was printed in literary journals such as "Blackwood's Edinburgh Magazine" and local newspapers. His first collection of poetry, "Prose Sketches and Poems Written in the Western Country", was published in 1834. He later gathered many of his poems and republished them in "Hymns to the Gods and Other Poems" (1872). After his death these were published again in "Gen. Albert Pike's Poems" (1900) and "Lyrics and Love Songs" (1916).
The authorship of "The Old Canoe" was attributed to Pike. He was suggested as author because about the time of its publication, when it was going the rounds of the press, probably without any credit, a doggerel called "The Old Canoe" was composed about Pike by one of his political foes. The subject was a canoe in which he left Columbia, Tennessee, when a young man practicing law in that place. Pike told Senator Edward W. Carmack that he was not the author of "The Old Canoe," and could not imagine how he ever got the credit for it. The rightful author was Emily Rebecca Page. Freemasonry. Pike first joined the fraternal Independent Order of Odd Fellows in 1840. He next joined a Masonic Lodge, where he became extremely active in the affairs of the organization. In 1859 he was elected Sovereign Grand Commander of the Scottish Rite's Southern Jurisdiction. He remained Sovereign Grand Commander for the rest of his life, devoting a large amount of his time to developing the rituals of the order. He published a book called "Morals and Dogma of the Ancient and Accepted Scottish Rite of Freemasonry" in 1871, the first of several editions. This helped the Order grow during the nineteenth century. He also researched and wrote the seminal treatise "Indo-Aryan Deities and Worship as Contained in the Rig-Veda". In the United States, Pike is still considered an eminent and influential Freemason, primarily in the Scottish Rite Southern Jurisdiction.
Pike was also the Provincial Grand Master of the Royal Order of Scotland from 1877 to 1891. Military service. Mexican–American War. When the Mexican–American War started, Pike joined the Arkansas Mounted Infantry Regiment and was commissioned as a company commander with the rank of captain in June 1846. With his regiment, he fought in the Battle of Buena Vista. Pike was discharged in June 1847. He and his commander, Colonel John Selden Roane, had several differences of opinion. This situation led finally to an "inconclusive" duel between Pike and Roane on July 29, 1847, near Fort Smith, Arkansas. Although several shots were fired in the duel, nobody was injured, and the two were persuaded by their seconds to discontinue it. After the war, Pike returned to the practice of law, moving to New Orleans for a time beginning in 1853. He wrote another book, "Maxims of the Roman Law and Some of the Ancient French Law, as Expounded and Applied in Doctrine and Jurisprudence". Although unpublished, this book increased his reputation among his associates in law. He returned to Arkansas in 1857, gaining some amount of prominence in the legal field.
At the Southern Commercial Convention of 1854, Pike said the South should remain in the Union and seek equality with the North, but if the South "were forced into an inferior status, she would be better out of the Union than in it." His stand was that state's rights superseded national law and he supported the idea of a Southern secession. This stand is made clear in his pamphlet of 1861, "State or Province, Bond or Free?" American Civil War. In 1861, Pike penned the lyrics to "Dixie to Arms!" At the beginning of the war, Pike was appointed as Confederate envoy to Native American nations. In this capacity he negotiated several treaties, one of the most important being with Cherokee chief John Ross, which was concluded in 1861. At the time, Ross agreed to support the Confederacy, which promised the tribes a Native American state if it won the war. Ross later changed his mind and left Indian Territory, but the succeeding Cherokee government maintained the alliance. Pike was commissioned as a brigadier general in the Confederate States Army on November 22, 1861, and given a command in the Indian Territory. With Brig. Gen. Ben McCulloch, Pike trained three Confederate regiments of Indian cavalry, most of whom belonged to the "civilized tribes", whose loyalty to the Confederacy was variable. Although initially victorious at the Battle of Pea Ridge (Elkhorn Tavern) in March 1862, Pike's unit was defeated later in a counterattack, after falling into disarray. When Pike was ordered in May 1862 to send troops to Arkansas, he resigned in protest. As in the previous war, Pike came into conflict with his superior officers, at one time drafting a letter to Jefferson Davis complaining about his direct superior.
After Pea Ridge, it was alleged that Pike's Native American troops had scalped soldiers in the field. The single incident of scalping was, however, done by a Native American acting on his own. Official records submitted to the Headquarters Department of Indian territory reveal that Pike "regarded [the incident] with horror" and that he was personally "angry and disgusted." He also filed a report in which he said it caused him the "utmost pain and regret." Maj. Gen. Thomas C. Hindman charged Pike with mishandling of money and material, ordering his arrest. The incident arose when Hindman, who had declared martial law in Arkansas, ordered Pike to turn over weapons and Native American Indian treaty funds. Pike thought the action was illegal and refused. Both these charges were later found to be considerably lacking in evidence; nevertheless Pike, facing arrest, escaped into the hills of Arkansas, submitting his resignation from the Confederate States Army on July 12, 1862. He was arrested on November 3 on charges of insubordination and treason, and held briefly in Warren, Texas. His resignation was accepted on November 11, and he was allowed to return to Arkansas.
As Union troops advanced toward the state capital in September 1863, the State Supreme Court retreated to Washington, Arkansas, which was made the new Confederate state capital. Associate Justice Hulbert F. Fairchild resigned because the new location was too far from his family, and Pike was appointed as his replacement. In the wake of the war, Pike moved to New York City, then for a short time to Canada. On June 24, 1865, Pike applied to President Andrew Johnson for a pardon, disowning his earlier interpretation of the U.S. Constitution. He said he now planned "to pursue the arts of peace, to practice my profession, to live among my books, and to labour to benefit my fellows and my race by other than political courses". President Johnson pardoned him on April 23, 1866. Later life and death. During the Arkansas political conflict known as the Brooks-Baxter War, Pike was one of the lawyers to speak on behalf of Elisha Baxter. Pike died on April 2, 1891, at the Scottish Rite Temple of the Supreme Council in Washington DC, at the age of 81, and was buried at Oak Hill Cemetery, despite the fact that he had left instructions for his body to be cremated. In 1944, his remains were moved to the House of the Temple, headquarters of the Southern Jurisdiction of the Scottish Rite. The House of the Temple contains numerous memorials and artifacts related to Pike, including his personal library.
Legacy. A memorial to Pike was erected in 1901 in the Judiciary Square neighborhood of Washington, D.C. The location was appropriate considering that Pike had sued the government to secure Native American rights. The statue portrayed him as a private citizen and Freemason. He was the only former Confederate military officer with an outdoor statue in Washington, D.C., and in 2019 Delegate Eleanor Holmes Norton called for its removal. On June 19, 2020, protesters tore down the statue and set it ablaze, in connection with the George Floyd protests because of Pike's association with the Confederacy and of his alleged association with the Ku Klux Klan. The Albert Pike Memorial Temple is an historic Masonic lodge in Little Rock, Arkansas; the structure is listed on the National Register of Historic Places. Albert Pike Highway was an auto trail that extended more than from Hot Springs, Arkansas, to Colorado Springs, Colorado, crossing the Ozark Mountains and passing through Fort Smith, Muskogee, Tulsa, Dodge City, La Junta and Pueblo.
Controversies. Masonic baptism. In 1865, Pike publicly performed a ceremony of Masonic baptism in New York City. The ceremony was greeted with skepticism by many American Masons including Albert Mackey, but was based on older European Masonic baptism ceremonies that began in the 1820s. However, some, like the "New York Times", reacted positively to the ceremony describing it as "interesting" and "novel." In the ritual, six children were baptized by Pike with water and consecrated oil. Racism. In the aftermath of the Civil War, as former Confederates found themselves barred from the ballot box, Pike remained deeply opposed to black suffrage, insisting that "the white race, and that race alone, shall govern this country. It is the only one that is fit to govern, and it is the only one that shall." Regarding membership in the Freemasons, Pike is quoted as saying, "Prince Hall Lodge was as regular a Lodge as any Lodge created by competent authority. It had a perfect right to establish other Lodges and make itself a Mother Lodge. I am not inclined to meddle in the matter. I took my obligations from white men, not from negroes. When I have to accept negroes as brothers or leave masonry, I shall leave it. Better let the thing drift."
His attitudes towards African-Americans may have changed towards the end of his life. A 1945 letter written by Willard W. Allen, the Sovereign Grand Commander of the United Supreme Council, S.J. Prince Hall Affiliation noted that "what practically all Masonic scholars know very well, viz., that in the closing years of General Pike's Masonic career, he became a very staunch friend of Negro Masonry." Pike had become a personal friend of Thornton A. Jackson, Supreme Grand Commander of the United Supreme Council, Southern Jurisdiction, Prince Hall Affiliation and even gifted to Thornton his complete set of rituals for Prince Hall Scottish Rite Masonry to use. Involvement with the Ku Klux Klan. Pike first wrote about the Ku Klux Klan less than three years after the Klan's founding, in an April 16, 1868 editorial in the "Memphis Daily Appeal." In the editorial, Pike indicated that his main problems lay not with its aims, but with its methods and leadership. Later in this editorial, he proposed "one great Order of Southern Brotherhood", a secret society which would have been a larger and more centrally organized version of the Klan: "If it were in our power, if it could be effected, we would unite every white man in the South, who is opposed to negro suffrage, into one great Order of Southern Brotherhood, with an organization complete, active, vigorous, in which a few should execute the concentrated will of all, and whose very existence should be concealed from all but its members."
In 1905's "Ku Klux Klan: Its Origin, Growth and Disbandment", the author Walter Lynwood Fleming, lists Pike as the Klan's "chief judicial officer". Susan Lawrence Davis, whose father was a founding member of the Klan in Alabama, writes in her sympathetic account titled "Authentic History: Ku Klux Klan, 1865–1877", published in 1924, that Pike was personally chosen by Nathan Bedford Forrest to serve as the Klan's "Chief Judicial Officer" and to head the Klan in Arkansas as "Grand Dragon of that Realm." In 1939's "Invisible Empire: The Story of the Ku Klux Klan, 1866–1871", Stanley Horn, who served as president of the Tennessee Historical Society, also reports that Forrest appointed Pike to lead the Klan in Arkansas and credits him with a surge of local Klan activity in April 1868. Horn says that a pro-Klan poem, "Death's Brigade", is attributed to Pike, although "of course, he did not have the bravado to claim that honor publicly at that time." Southern Agrarian poet John Gould Fletcher, who grew up in Little Rock in a house that Pike built, likewise believed that Pike wrote the poem.
When the Ku Klux Klan was revived in 1915, there even existed an Albert Pike Klan, a local chapter of the organization based in Illinois. In 1971, Allen W. Trelease published "White Terror: The Ku Klux Klan Conspiracy and Southern Reconstruction", and claimed that the office that Pike allegedly held in the KKK was not mentioned in "The Prescript", the Klan constitution. However, the office of Grand Dragon, which Davis claims Pike once held, is explicitly mentioned in the 1867 Klan constitution. At the same time, Trelease noted that "Pike may well have affiliated with the Klan." As evidence, Trelease notes that Pike "was intrigued by secret societies and rituals" and "sympathized with the Klan's stated objectives." In his 1997 biography of Pike, Walter Lee Brown asserts that Pike was not a member of the Klan and Brown found "no contemporary, nor no reliable late evidence that Pike ever joined the Klan." Brown claims the work of Fleming, Davis and Horn are "unreliable histories", but offers no further evidence other than citing Trelease, which, in Brown's interpretation "cast's doubt on Pike's membership."
ALF Tales ALF Tales is a 30-minute Saturday morning animated series that aired on NBC from September 10, 1988, to December 9, 1989. The show is a spin-off of "" that featured characters from that series playing various characters from fairy tales. The fairy tale parody was usually altered for comedic effect in a manner akin to Jay Ward's "Fractured Fairy Tales". The episodes were performed in the style of a resident theater company or ensemble cast where Gordon and Rhonda would take the leading male and female roles, and the other characters were cast according to their characteristics. Many stories spoof a film genre, such as the "Cinderella" episode which is presented like an Elvis Presley film. Some episodes featured a "fourth wall" effect where Gordon is backstage preparing for the episode, and Rob Cowan would appear drawn as a TV executive (who introduced himself as "Roger Cowan, network executive") who tries to brief Gordon on how to improve the episode. For instance Cowan once told Gordon who was readying for a medieval themed episode that "less than 2% of our audience lives in the Dark Ages".
Home media. The first seven episodes were released on DVD on May 30, 2006, in Region 1 from Lionsgate Home Entertainment in a single-disc release entitled "ALF and The Beanstalk and Other Classic Fairy Tales". The complete series was remastered and subsequently released on October 17, 2023, by Shout! Factory in the DVD box set "ALF: The Complete Series (Deluxe Edition)". The box set release also included the original 1986–90 sitcom, ' and '.
Abdul Rashid Dostum Abdul Rashid Dostum ( ; ; Uzbek Latin: , Uzbek Cyrillic: , ; born 25 March 1954) is an Afghan former military officer, warlord and exiled politician. He is the founder and leader of the Junbish-e Milli political party, and was a senior army officer in the military of the former Afghan communist government during the Soviet–Afghan War, initially part of the Afghan Commando Forces. In 2001, Dostum became a key indigenous ally to the U.S. Special Forces and the CIA during the campaign to topple the Taliban government. He is one of the most powerful warlords since the beginning of the Afghan conflict, infamous for being an opportunist and siding with winners during Afghanistan's several civil wars. Dostum was also referred to as a kingmaker due to his significant role in Afghan politics. An ethnic Uzbek from a peasant family in Jawzjan province, Dostum joined the People's Democratic Party of Afghanistan (PDPA) as a teenager before enlisting in the Afghan Army and training as a paratrooper, serving in his native region around Sheberghan. Following the outbreak of the Soviet–Afghan War, Dostum commanded a KHAD paramilitary unit and eventually gained a reputation for defeating mujahideen commanders in northern Afghanistan and even persuading some to defect to the communist cause. As a result, the communist government gained effective control over the country's north. He achieved several promotions in the army and was honored as a "Hero of Afghanistan" by President Mohammed Najibullah in 1988. By this time he was commanding up to 45,000 troops in the region under his responsibility.
Following the dissolution of the Soviet Union, Dostum played a central role in the collapse of Najibullah's government by "defecting" to the mujahideen; the division-sized loyal forces he commanded in the north became an independent paramilitary of his newly founded party called Junbish-e Milli. He allied with Ahmad Shah Massoud and together they captured Kabul, before another civil war loomed. Initially supporting the new government of Burhanuddin Rabbani, he switched sides in 1994 by allying with Gulbuddin Hekmatyar, but he backed Rabbani again by 1996. During this time he remained in control of the country's north which functioned as a relatively stable proto-state, but remained a loose partner of Massoud in the Northern Alliance. A year later, Mazar-i-Sharif was overrun by his former aide Abdul Malik Pahlawan, resulting in a battle in which he regained control. In 1998, the city was overrun by the Taliban and Dostum fled the country until returning to Afghanistan in 2001, joining the Northern Alliance forces after the US invasion and leading his loyal faction in the Fall of Mazar-i-Sharif.
After the fall of the Taliban-led government, he joined interim president Hamid Karzai's administration as Deputy Defense Minister and later served as chairman Joint Chiefs of Staff of the Afghan Army, a role often viewed as ceremonial. His militia feuded with forces loyal to general Atta Muhammad Nur. Dostum was a candidate in the 2004 elections, and was an ally of victorious Karzai in the 2009 elections. From 2011, he was part of the leadership council of the National Front of Afghanistan along with Ahmad Zia Massoud and Mohammad Mohaqiq. He served as Vice President of Afghanistan in Ashraf Ghani's administration from 2014 to 2020. In 2020, he was promoted to the rank of marshal after a political agreement between Ghani and former Chief Executive Abdullah Abdullah. On 11 August 2021 during the Taliban's nationwide offensive, Dostum fled across Hairatan to Uzbekistan. In 2021 he pledged allegiance to the National Resistance Front of Afghanistan, and formed the "Supreme Council of National Resistance of the Islamic Republic of Afghanistan" in opposition to the new Taliban government. Dostum now resides in exile in Turkey, and on 15 September 2024 urged groups opposed to Taliban rule to form a government-in-exile.
Dostum is a controversial figure in Afghanistan. He is seen as a capable and fierce military leader and remains wildly popular among the Uzbek community in the country. Many of his supporters call him "Pasha" (پاشا), an honorable Uzbek/Turkic term. However, he has also been widely accused of committing atrocities and war crimes, most notoriously the suffocation of up to 1,000 Taliban fighters in the Dasht-i-Leili massacre, and he was widely feared among the populace. In 2018, the International Criminal Court (ICC) was reported to be considering launching an inquiry into whether Dostum had engaged in war crimes in Afghanistan. Early life. Dostum was born in 1954 in Khwaja Du Koh near Sheberghan in Jowzjan province, Afghanistan. Coming from an impoverished ethnic Uzbek family, he received a very basic traditional education as he was forced to drop out of school at a young age. From there, he took up work in the village's major gas fields. Career. Dostum began working in 1970 in a state-owned gas refinery in Sheberghan. He began participating in union politics when the republican government led by Daoud Khan started to arm the staff of the workers in the oil and gas refineries. The reason for this was to create "groups for the Defense of the Revolution". As a result of the new communist ideas entering Afghanistan in the 1970s, Dostum enlisted in the Afghan Army in 1976. He received his basic military training in Jalalabad and his squadron was deployed in the rural areas around Sheberghan, under the auspices of the Ministry of National Security. According to photographic evidence, Dostum additionally received airborne training and still chooses to wear two first-class "Master Paratrooper" Afghan jump wings.
As a Parcham faction member of the People's Democratic Party of Afghanistan (PDPA), he was exiled after the purge of Parcham by the party's Khalqist faction leaders, living in Peshawar, Pakistan for a while. After the Soviet invasion (Operation Storm-333) and installation of Babrak Karmal as head of state, Dostum returned to Afghanistan, where he started commanding a local pro-government militia in his native Jawzjan Province. Soviet–Afghan War. By the mid-1980s, he commanded a force of around 20,000 paramilitaries and controlled the northern provinces of Afghanistan. While the unit recruited throughout Jowzjan and had a relatively broad base, many of its early troops and commanders came from Dostum's home village. He left the army after the purge of Parchamites, but returned after the Soviet occupation began. During the Soviet–Afghan War, Dostum was commanding a paramilitary battalion to fight and route mujahideen forces; he had been appointed an officer due to prior military experience. This eventually became a regiment and later became incorporated into the defense forces as the 53rd Infantry Division. Dostum and his new division reported directly to President Mohammad Najibullah. Later on, he became the commander of the military unit 374 in Jowzjan. He defended the Soviet-backed Afghan government against the mujahideen forces throughout the 1980s. While he was only a regional commander, he had largely raised his forces by himself. The Jowzjani militia Dostum controlled was one of the few in the country that was able to be deployed outside its own region. They were deployed in Kandahar in 1988 when Soviet forces were withdrawing from Afghanistan.
Due to his efforts in the army, Dostum was awarded the title "Hero of the Republic of Afghanistan" by President Najibullah. Civil war and northern Afghanistan autonomous state. Dostum's men would become an important force in the fall of Kabul in 1992, with Dostum deciding to defect from Najibullah and allying himself with opposition commanders Ahmad Shah Massoud and Sayed Jafar Naderi, the head of the Isma'ili community, and together they captured the capital city. With the help of fellow defectors Mohammad Nabi Azimi and Abdul Wakil, his forces entered Kabul by air in the afternoon of 14 April. He and Massoud fought in a coalition against Gulbuddin Hekmatyar. Massoud and Dostum's forces joined to defend Kabul against Hekmatyar. Some 4,000–5,000 of his troops, units of his Sheberghan-based 53rd Division and Balkh-based Guards Division, garrisoning Bala Hissar fort, Maranjan Hill and Khwaja Rawash Airport, where they stopped Najibullah from entering to flee. Dostum then left Kabul for his northern stronghold Mazar-i-Sharif, where he ruled, in effect, an independent region (or 'proto-state'), often referred as the Northern Autonomous Zone. He printed his own Afghan currency, ran a small airline named Balkh Air, and formed relations with countries like Uzbekistan effectively creating his own proto-state with an army of up to 40,000 men, and with tanks supplied by Uzbekistan and Russia. While the rest of the country was in chaos, his region remained prosperous and functional, and it won him the support from people of all ethnic groups. Many people fled to his territory to escape the violence and fundamentalism imposed by the Taliban later on. In 1994, Dostum allied himself with Gulbuddin Hekmatyar against the government of Burhanuddin Rabbani and Ahmad Shah Massoud, but in 1995 sided with the government again.
Taliban era. Following the rise of the Taliban and their capture of Kabul, Dostum aligned himself with the Northern Alliance (United Front) against the Taliban. The Northern Alliance was assembled in late 1996 by Dostum, Massoud and Karim Khalili against the Taliban. At this point, he is said to have had a force of some 50,000 men supported by both aircraft and tanks. Much like other Northern Alliance leaders, Dostum also faced infighting within his group and was later forced to surrender his power to General Abdul Malik Pahlawan. Malik entered into secret negotiations with the Taliban, who promised to respect his authority over much of northern Afghanistan, in exchange for the apprehension of Ismail Khan, one of their enemies. Accordingly, on 25 May 1997, Malik arrested Khan, handed him over and let the Taliban enter Mazar-e-Sharif, giving them control over most of northern Afghanistan. Because of this, Dostum was forced to flee to Turkey. However, Malik soon realized that the Taliban were not sincere with their promises as he saw his men being disarmed. He then rejoined the Northern Alliance and turned against his erstwhile allies, driving them from Mazar-e-Sharif. In October 1997, Dostum returned from exile and retook charge. After Dostum briefly regained control of Mazar-e-Sharif, the Taliban returned in 1998, and he again fled to Turkey.
Operation Enduring Freedom. Dostum returned to Afghanistan in May 2001 to open up a new front before the U.S.-led campaign against the Taliban joined him, along with Commander Massoud, Ismail Khan and Mohammad Mohaqiq. On 17 October 2001, the CIA's eight-man Team Alpha, including Johnny Micheal Spann landed in the Dar-e-Suf to link up with Dostum. Three days later, the 12 members of Operational Detachment Alpha (ODA) 595 landed to join forces with Dostum and Team Alpha. Dostum, the Tajik commander Atta Muhammad Nur and their American allies defeated Taliban forces and recaptured Mazar-i-Sharif on 10 November 2001. On 24 November 2001, 15,000 Taliban soldiers were due to surrender after the Siege of Kunduz to American and Northern Alliance forces. Instead, 400 Al-Qaeda prisoners arrived just outside Mazar-i-Sharif. After they surrendered to Dostum, they were transferred to the 19th century garrison fortress, Qala-i-Jangi. The next day, while being questioned by CIA officers Spann and David Tyson, they used concealed weapons to revolt, triggering what became the Battle of Qala-i-Jangi against the guards. The uprising was finally brought under control after six days.
Dasht-i-Leili massacre. Dostum has been accused by Western journalists of responsibility for the suffocating or otherwise killing of Taliban prisoners in December 2001, with the number of victims estimated as 2,000. In 2009, Dostum denied the accusations and US President Obama ordered an investigation into the massacre. Karzai administration. In the aftermath of Taliban's removal from northern Afghanistan, forces loyal to Dostum frequently clashed with Tajik forces loyal to Atta Muhammad Nur. Atta's men kidnapped and killed a number of Dostum's men, and constantly agitated to gain control of Mazar-e-Sharif. Through the political mediations of the Karzai administration, the International Security Assistance Force (ISA) and the United Nations, the Dostum-Atta feud gradually declined, leading to their alignment in a new political party. Dostum served as deputy defense minister the early period of the Karzai administration. On 20 May 2003, Dostum narrowly escaped an assassination attempt. He was often residing outside Afghanistan, mainly in Turkey. In February 2008, he was suspended after the apparent kidnapping and torture of a political rival.
Time in Turkey. Some media reports in 2008 stated earlier that Dostum was "seeking political asylum" in Turkey while others said he was exiled. One Turkish media outlet said Dostum was visiting after flying there with then Turkey's Foreign Minister Ali Babacan during a meeting of the Organization for Security and Cooperation in Europe (OSCE). On 16 August 2009, Dostum was asked to return from exile to Afghanistan to support President Hamid Karzai in his bid for re-election. He later flew by helicopter to his northern stronghold of Sheberghan, where he was greeted by thousands of his supporters in the local stadium. He subsequently made overtures to the United States, promising he could "destroy the Taliban and al Qaeda" if supported by the U.S., saying that "the U.S. needs strong friends like Dostum." Ghani administration. On 7 October 2013, the day after filing his nomination for the 2014 general elections as running mate of Ashraf Ghani, Dostum issued a press statement that some news media were willing to welcome as "apologies": "Many mistakes were made during the civil war (…) It is time we apologize to the Afghan people who were sacrificed due to our negative policies (…) I apologize to the people who suffered from the violence and civil war (…)".
Dostum was directly chosen as First Vice President of Afghanistan in the April–June 2014 Afghan presidential election, next to Ashraf Ghani as president and Sarwar Danish as second vice president. In July 2016, Human Rights Watch accused Abdul Rashid Dostum's National Islamic Movement of Afghanistan of killing, abusing and looting civilians in the northern Faryab Province during June. Militia forces loyal to Dostum stated that the civilians they targeted – at least 13 killed and 32 wounded – were supporters of the Taliban. In November 2016, at a buzkashi match, he punched his political rival Ahmad Ischi, and then his bodyguards beat Ischi. In 2017, he was accused of having Ischi kidnapped in that incident and raped with a gun on camera during a five-day detention, claims that Dostum denies but that nevertheless forced him into exile in Turkey. On 26 July 2018, he narrowly escaped a suicide bombing by ISIL-KP as he returned to Afghanistan at Kabul airport. Just after Dostum's convoy departed the airport, an attacker armed with a suicide vest bombed a crowd of several hundred people celebrating his return at the entrance to the airport. The attack killed 14 and injured 50, including civilians and armed security.
On 30 March 2019, Dostum again escaped an expected assassination attempt while traveling from Mazar-e-Sharif to Jawzjan Province, though two of his bodyguards were killed. The Taliban claimed responsibility for the attack, the second in eight months. On 11 August 2021 during the Taliban's nationwide offensive, Dostum, along with Atta Muhammad Nur, led the government's defence of the city of Mazar-i-Sharif. Three days later, they fled across Hairatan to Uzbekistan. Atta Nur claimed that they were forced to flee due to a "conspiracy". Both men later pled allegiance to the National Resistance Front of Afghanistan, the remaining remnants of the collapsed Islamic Republic of Afghanistan. Dostum, Atta, Yunus Qanuni, Abdul Rasul Sayyaf and some other political figures formed the "Supreme Council of National Resistance of the Islamic Republic of Afghanistan" in opposition to the new Taliban government in October 2021. Dostum now resides in exile in Turkey, and on 15 September 2024 urged groups opposed to Taliban rule to form a government-in-exile.
Political and social views. Dostum is considered to be liberal and somewhat leftist. Being ethnic Uzbek, he has worked on the battlefield with leaders from all other major ethnic groups, Hazaras, Tajiks and Pashtuns. When Dostum was ruling his northern Afghanistan proto-state before the Taliban took over in 1998, women were able to go about unveiled, girls were allowed to go to school and study at the University of Balkh, cinemas showed Indian films, music played on television, and Russian vodka and German beer were openly available: activities which were all banned by the Taliban. He viewed the ISAF forces attempt to crush the Taliban as ineffective and has gone on record saying in 2007 that he could mop up the Taliban "in six months" if allowed to raise a 10,000 strong army of Afghan veterans. As of 2007, senior Afghan government officials did not trust Dostum as they were concerned that he might be secretly rearming his forces. Personal life. Dostum is more than tall and has been described as "beefy". He generally prefers to wear a Soviet-style camouflage military uniform, previously having worn KLMK, having a trademark bushy moustache.
Dostum was married to a woman named Khadija. According to Brian Glyn Williams, Khadija had an accidental death in the 1990s which broke Dostum as he "really loved his wife". Dostum eventually remarried after Khadija's death. He named one of his sons Mustafa Kamal, after the founder of the modern Turkish Republic, Mustafa Kemal Atatürk. Dostum has spent a considerable amount of time in Turkey, and some of his family reside there. Dostum is known to drink alcohol, a rarity in Afghanistan, and apparently a fan of Russian vodka. He reportedly suffered from diabetes. In 2014 when he became vice president, Dostum reportedly gave up drinking for healthy meals and morning jogs.
Andhra Pradesh Andhra Pradesh (, AP) is a state on the east coast of southern India. It is the seventh-largest state and the tenth-most populous in the country. Telugu is the most widely spoken language in the state, as well as its official language. Amaravati is the state capital, while the largest city is Visakhapatnam. Andhra Pradesh shares borders with Odisha to the northeast, Chhattisgarh to the north, Karnataka to the southwest, Tamil Nadu to the south, Telangana to northwest and the Bay of Bengal to the east. It has the second-longest coastline in India at about . Archaeological evidence indicates that Andhra Pradesh has been continuously inhabited for over 247,000 years, from early archaic hominins to Neolithic settlements. The earliest reference to the Andhras appears in the Aitareya Brahmana () of the Rigveda. Around 300 BCE, the Andhras living in the Godavari and Krishna river deltas were renowned for their formidable military strength—second only to the Maurya Empire in the subcontinent. The first major Andhra polity was the Satavahana dynasty (2nd century BCE–2nd century CE) which ruled over the entire Deccan Plateau and even distant areas of western and central India. They established trade relations with the Roman Empire, and their capital, Dhanyakataka, was the most prosperous city in India during the 2nd century CE. Subsequent major dynasties included the Vishnukundinas, Eastern Chalukyas, Kakatiyas, Vijayanagara Empire, and Qutb Shahis, followed by British rule. After gained independence, Andhra State was carved out of Madras State in 1953. In 1956, it merged with Telangana, comprising the Telugu-speaking regions of the former Hyderabad State, to form Andhra Pradesh. It reverted to its earlier form in 2014, when the new state of Telangana was bifurcated from it.
The Eastern Ghats separate the coastal plains from the peneplains. Major rivers include the Krishna, Godavari, Tungabhadra and Penna. Andhra Pradesh holds about one-third of India's limestone reserves and significant deposits of baryte and granite. Agriculture and related activities employ 62.17% of the population, with rice being the staple crop. The state contributes 30% of India’s fish production and accounts for 35% of the country’s seafood exports. The Sriharikota Range, located on Sriharikota island in Tirupati district, serves as India's primary satellite launch centre. Andhra is the birthplace of the Amaravati school of art, an ancient Indian art style that influenced South Indian, Sri Lankan, and Southeast Asian art. It is also home to Kuchipudi, one of India’s classical dance forms, and has produced several renowned Carnatic music composers. The state features prominent pilgrimage centres and natural attractions, including the Venkateswara temple in Tirumala and the Araku Valley. Notable products with geographical indication (GI) registration include Tirupati Laddu, Banganapalle mangoes, Kondapalli toys, Dharmavaram sarees, and Pootharekulu.
Etymology. Andhras were mentioned in Aitareya Brahmana of the Rigveda () as descendants of Sage Vishvamitra. Andhras were referred to as non-aryans living on the fringes of Aryan settlements. The Satavahanas, the earliest kings who ruled this region were mentioned by the names "Andhra", "Andhrara-jateeya", and "Andhrabhrtya" in the Puranic literature. "Andhra" is both a tribal and territorial name. The region inhabited by Andhras was called Andhradesa. History. Pre-history. Excavated stone tools from Hanumanthunipadu in Prakasam district were dated to be 2.47 lakh (247,000) years old (Middle Paleolithic). This finding means that these tools were developed by archaic hominins (pre-humans) as such tools were thought to be used by modern man migrating out of Africa about 2.10 lakh (210,000) years ago. In the absence of fossil evidence, it became a riddle to understand what happened to them. The discovery of petroglyphs, pictographs, and dolmens in Chakrala Bodu, near Boyalapalli village in the Yerragondapalem mandal area of Prakasam district indicates the presence of Neolithic-age human settlements in the region.
Early and medieval history. Megasthenes reported in his "Indica" () that Andhras were living in the Godavari and Krishna river deltas and were famous for their military strength which was second only to Mauryans in all of India. Archaeological evidence from places such as Bhattiprolu, Amaravathi, and Dharanikota suggests that the Andhra region was part of the Mauryan empire. After the death of Emperor Ashoka, Mauryan rule weakened around 200 BCE and was replaced by several smaller kingdoms in the Andhra region. One of the earliest examples of the Brahmi script, from Bhattiprolu was used on an urn containing the relics of Buddha. This is considered as the rosetta stone for decipherment of Tamil Brahmi. Kadamba script, derived from Bhattiprolu Brahmi later led to the evolution of Telugu and Kannada scripts. The Satavahana dynasty dominated the Deccan Plateau from the 1st century BCE to the 3rd century CE. It had trade relations with the Roman Empire. The Satavahanas made Dhanyakataka-Amaravathi their capital. According to historian Stanley Wolpert, it might have been the most prosperous city in India in 2nd century CE. Nagarjuna, the philosopher of Mahayana, lived in this region. Mahayana spread to China, Japan, and Korea. It became the largest Buddhist denomination in the world. Amaravati School of Art is regarded as one of the three major styles of ancient Indian art and had a great influence on art in South India, Sri Lanka, and Southeast Asia. The Andhra Ikshvakus, with their capital at Vijayapuri, succeeded the Satavahanas in the Krishna River valley in the latter half of the 2nd century CE. The Salankayanas were an ancient dynasty that ruled the Andhra region between Godavari and Krishna rivers with their capital at Vengi (modern Pedavegi) around 300 CE. Telugu Cholas ruled present-day Kadapa region from the six to the thirteenth centuries intermittently. Kallamalla "sasanam" (law), engraved in 575 CE during the rule of Dhanamjaya, is the earliest completely Telugu inscription.
The Vishnukundinas was the first dynasty in the fifth and sixth centuries to hold sway over South India. Undavalli Caves is an example of Indian rock-cut architecture of that time. The Eastern Chalukyas of Vengi, whose dynasty lasted for around five hundred years from the 7th century until 1130 CE, eventually merged with the Chola dynasty. They continued to rule under the protection of the Chola dynasty until 1189 CE. At the request of King Rajaraja Narendra, Nannaya, considered the first Telugu poet, took up the translation of the Mahabharata into Telugu in 1025 CE. Kakatiyas ruled this region and Telangana for nearly two hundred years between the 12th and 14th centuries. They were defeated by the Delhi Sultanate. Bahamani sultanate took over when Delhi sultanate became weak. Around the same time, Musunuris and Reddi Kingdom ruled parts of this region in the early 14th century. Reddy kings constructed Kondaveedu Fort and Kondapalli Fort. Gajpathis ruled parts of this region, before the entire region became part of the Vijayanagara Empire during the reign of Krishnadevaraya. Pemmasani Nayaks controlled parts of Andhra Pradesh and had large mercenary armies that were the vanguard of the empire in the 16th century. Several tanks and anicuts were built. Some of these include the Cumbum tank, Mopad tank, and Koregal anicut, Vallabhapur anicut across the Tungabhadra River. The empire's patronage enabled fine arts and literature to reach new heights in Kannada, Telugu, Tamil, and Sanskrit, while Carnatic music evolved into its current form. The Lepakshi group of monuments built during this period have mural paintings of the Vijayanagara kings, Dravidian art, and inscriptions. These are put on the tentative list of the UNESCO World Heritage committee.
Modern history. Following the defeat of the Vijayanagara Empire, the Qutb Shahi dynasty held sway over most of present-day general Andhra Pradesh and Telangana. Later, this region came under the rule of the Mughal Empire. Chin Qilich Khan who was initially appointed as viceroy of Deccan by the Mughal in 1713, established himself as a semi-independent ruler as Nizam of Hyderabad . In 1765, British Lord Robert Clive obtained from the Mughal emperor Shah Alam II a grant of four circars to the British East India Company, that was formalised in 1778 treaty with Nizam Ali, the 5th Nizam of Hyderabad state with addition of another circar. Later, four territories were ceded to the British by Nizam Ali in 1800, which eventually became the Rayalaseema region. Meanwhile, in the present day North Andhra, Raja Viziaram Raz (Vijayaram Raj) established a sovereign kingdom and acquired neighbouring estates with the support of British. Later it fell out with the British and, as a result, was attacked and defeated in the battle of Padmanabham in 1794. It was annexed as a tributary estate like other principalities and remained so until it acceded to the Indian Union in 1949. Following the annexation of Carnatic sultanate in 1801, the last major piece of the present day Andhra Pradesh came under British East India Company rule as part of Madras Presidency. After the Indian Rebellion of 1857, the region became part of British crown till India became independent in 1947.
Anicut at Dowleswaram built in 1850 by Arthur Cotton and several others at Vijayawada, Nellore, Sangam, Sunkesula, and Polampalli are examples of irrigation facilities built during the British raj, that irrigated lacs of acres across coastal districts. Buckingham Canal built during 1806–1878, running parallel to the Coramandal coast from Kakinada to Marakkanam in Tamil Nadu served as a major water transportation route for goods till the 1960s. Telegraph service initiated in 1850 served for over 160 years till it was stopped on 15 July 2013 citing poor patronage due to advances in mobile communications and short message service. Charles Philip Brown did pioneering work in transforming Telugu to the print era and introduced Vemana poems to English readers. Kandukuri Veeresalingam is considered the father of the Telugu renaissance movement, as he encouraged the education of women and lower caste people. He fought against Brahmin marriage customs such as child marriage, the bride price system, and the prohibition of widow remarriage.
To gain an independent state based on linguistic identity and to protect the interests of the Telugu-speaking people of Madras State, Potti Sreeramulu fasted to death in 1952. The Telugu-speaking area of Andhra State was carved out of Madras state on 1 October 1953, with Kurnool as its capital city. On the basis of the Gentlemen's Agreement of 1956, the States reorganisation act created Andhra Pradesh by merging the neighbouring Telugu-speaking areas of the Hyderabad State with Hyderabad as the capital on 1 November 1956. Hyderabad grew rapidly partly through investments flowing in from agrarian change and 'green revolution' in coastal Andhra. In the unified state, Indian National Congress (INC) enjoyed a monopoly in ruling the state till 1983. After that Telugu Desam Party (TDP) led by N. T. Rama Rao (NTR) came to power and became another major party to rule the state. Nagarjuna Sagar Dam commissioned in 1967, Srisailam hydro electric project commissioned in 1982 are few examples of irrigation and electricity projects. The increased presence of women, Dalits, and tribals in the social and political spheres of the state, driven by social movements, led to a rise in violence against these groups. Securing access to resources like land remains an unachieved objective in the effort to expand their economic opportunities in the state. When the union cabinet decided to consider the formation of Telangana state in 2009 heeding to the demand of relaunched Telangana movement, Samaikyandhra Movement opposing it took shape and the state went through a turmoil. Finally, the Andhra Pradesh reorganisation act bill was passed by the parliament of India for the formation of the Telangana state, despite opposition by the state legislature. The new state of Telangana came into existence on 2 June 2014 after approval from the president of India, with the residual state continuing as Andhra Pradesh.
TDP formed the first government of the residual state with Chandrababu Naidu as chief minister. In 2017, the government of Andhra Pradesh began operating from its new greenfield capital, Amaravati, for which 33,000 acres were acquired from farmers through an innovative land pooling scheme. In the 2019 elections, Y. S. Jagan Mohan Reddy, leader of the YSR Congress Party, became the chief minister by winning 151 out of 175 seats. He introduced the 'village and ward volunteers' system, and reorganised the state with 26 districts. Introduction of English as the medium of instruction was done in almost all the state schools. The move to three capitals with Amaravati getting reduced to being the legislative capital, Vizag as the executive capital and Kurnool as the judicial capital was stuck down by the High Court. His government appealed to the Supreme Court. Geography. Andhra Pradesh is the seventh-largest state with an area of . Politically, the state shares borders with Orissa to the northeast, Chhattisgarh to the north, Karnataka to the southwest, Tamil Nadu to the south, Telangana to northwest and the Bay of Bengal to the east. Yanam district, an enclave of Puducherry, is in the state bordering Kakinada district. It has a coastline of around , which makes it the second-longest coastline in the nation.
The Eastern Ghats are a major dividing line separating coastal plains and peneplains in the state's geography. These are discontinuous, and individual sections have local names. The ghats become more pronounced towards the south and extreme north of the coast. Some of these consist of the Horsley Hills, the Seshachala Hills, the Nallamala Hills, and the Papi Hills. Arma Konda, located in Visakhapatnam district, is the highest peak in the state. Peneplains, part of Rayalaseema, slope towards the east. The Eastern Coastal Plains comprise the area of coastal districts up to the Eastern ghats as their border along the Bay of Bengal, with variable width. These are, for the most part, delta regions formed by the Krishna, Godavari, and Penna rivers. The state has five different soil types with the majority soil types being red lateritic and black soil types. Most of the coastal plains are put to intensive agricultural use. The Kadapa basin, formed by two arching branches of the Eastern ghats, is a mineral-rich area.

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