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"schema_version": "1.0",
"level": "species",
"genus": "Acinetobacter",
"species": "johnsonii",
"overview": {
"short": "Acinetobacter johnsonii is a Gram-negative, aerobic, non-fermentative coccobacillus commonly recovered from environmental sources.",
"clinical_context": "Rarely associated with human infection and typically considered of low pathogenic potential."
},
"expected_fields": {
"Gram Stain": "Negative",
"Shape": "Coccobacilli",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Media Grown On": [
"Blood Agar",
"MacConkey Agar"
],
"Colony Morphology": [
"Smooth",
"Grey",
"Dry"
],
"Oxygen Requirement": "Aerobic",
"Growth Temperature": "15//37",
"Catalase": "Positive",
"Oxidase": "Negative",
"Indole": "Negative",
"Urease": "Negative",
"Citrate": "Negative",
"Methyl Red": "Negative",
"VP": "Negative",
"H2S": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"Coagulase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"Nitrate Reduction": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Ornitihine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Glucose Fermentation": "Negative",
"Lactose Fermentation": "Negative",
"Sucrose Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Gas Production": "Negative",
"TSI Pattern": "K/K",
"Colony Pattern": "Unknown",
"Pigment": "None",
"Motility Type": "None"
},
"species_markers": [
{
"field": "Growth Temperature",
"value": "37//37",
"importance": "medium",
"notes": "Does not tolerate higher temperatures typical of A. baumannii."
}
],
"common_confusions": [
{
"species": "Acinetobacter lwoffii",
"reason": "Similar low biochemical activity and environmental presence."
}
],
"when_to_question_identification": [
"Isolation from severe clinical infections may indicate another species.",
"Growth at elevated temperatures argues against A. johnsonii."
],
"recommended_next_tests": [
{
"test": "MALDI-TOF MS",
"reason": "Definitive species-level identification."
}
]
} |