File size: 3,605 Bytes
03d2dca c4f36eb 03d2dca c4f36eb 03d2dca c4f36eb 03d2dca c4f36eb 03d2dca c4f36eb 03d2dca c4f36eb 03d2dca c4f36eb 03d2dca c4f36eb |
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 |
{
"schema_version": "1.0",
"level": "species",
"genus": "Comamonas",
"species": "testosteroni",
"overview": {
"short": "Comamonas testosteroni is a Gram-negative, aerobic, non-fermentative rod primarily associated with environmental sources.",
"clinical_context": "Rare opportunistic pathogen occasionally isolated from bloodstream, respiratory, or wound infections."
},
"expected_fields": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Media Grown On": [
"Blood Agar",
"Nutrient Agar"
],
"Colony Morphology": [
"Smooth",
"Grey",
"Non-pigmented"
],
"Oxygen Requirement": "Aerobic",
"Growth Temperature": "20//42",
"Catalase": "Positive",
"Oxidase": "Positive",
"Indole": "Negative",
"Urease": "Negative",
"Citrate": "Positive",
"Methyl Red": "Negative",
"VP": "Negative",
"H2S": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"Coagulase": "Negative",
"Gelatin Hydrolysis": "Variable",
"Esculin Hydrolysis": "Negative",
"Nitrate Reduction": "Positive",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Ornitihine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Glucose Fermentation": "Negative",
"Lactose Fermentation": "Negative",
"Sucrose Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Gas Production": "Negative",
"TSI Pattern": "Unknown",
"Colony Pattern": "Unknown",
"Pigment": "None",
"Motility Type": "Polar"
},
"species_markers": [
{
"field": "Citrate",
"value": "Positive",
"importance": "high",
"notes": "Major discriminator from Comamonas kerstersii."
},
{
"field": "Nitrate Reduction",
"value": "Positive",
"importance": "high",
"notes": "Supports identification when combined with citrate positivity."
},
{
"field": "Environmental Source",
"value": "Common",
"importance": "medium",
"notes": "Frequently isolated from soil and water."
}
],
"common_confusions": [
{
"species": "Comamonas kerstersii",
"reason": "Overlapping morphology and non-fermentative biochemistry."
},
{
"genus": "Achromobacter",
"reason": "Shared oxidase-positive, non-fermentative profile."
}
],
"when_to_question_identification": [
"Citrate negativity suggests Comamonas kerstersii.",
"Clinical isolation from intra-abdominal infections favors C. kerstersii.",
"Glucose fermentation excludes Comamonas species."
],
"recommended_next_tests": [
{
"test": "Citrate utilization",
"reason": "Primary biochemical discriminator.",
"api_kit": "API 20NE"
},
{
"test": "Nitrate reduction",
"reason": "Supports species-level separation.",
"api_kit": "API 20NE"
},
{
"test": "MALDI-TOF MS",
"reason": "Definitive species identification.",
"api_kit": "Not applicable"
}
]
} |