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Update data/rag/knowledge_base/Anaerococcus/vaginalis.json
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{
"schema_version": "1.0",
"level": "species",
"genus": "Anaerococcus",
"species": "vaginalis",
"overview": {
"short": "Anaerococcus vaginalis is a Gram-positive anaerobic coccus associated primarily with the female genitourinary tract.",
"clinical_context": "Most frequently isolated from vaginal and pelvic infections, often as part of mixed anaerobic flora."
},
"expected_fields": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Motility": "Negative",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Haemolysis": "Variable",
"Haemolysis Type": "Variable",
"Media Grown On": [
"Blood Agar",
"Anaerobic Blood Agar"
],
"Colony Morphology": [
"Small",
"Grey",
"Smooth"
],
"Oxygen Requirement": "Anaerobic",
"Growth Temperature": "35//37",
"Catalase": "Negative",
"Oxidase": "Negative",
"Indole": "Variable",
"Urease": "Negative",
"Citrate": "Negative",
"Methyl Red": "Unknown",
"VP": "Unknown",
"H2S": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"Coagulase": "Negative",
"Gelatin Hydrolysis": "Variable",
"Esculin Hydrolysis": "Negative",
"Nitrate Reduction": "Variable",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Ornitihine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Glucose Fermentation": "Positive",
"Lactose Fermentation": "Variable",
"Sucrose Fermentation": "Variable",
"Maltose Fermentation": "Variable",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Trehalose Fermentation": "Variable",
"Inositol Fermentation": "Negative",
"Gas Production": "Variable",
"TSI Pattern": "Unknown",
"Colony Pattern": "Unknown",
"Pigment": "None",
"Motility Type": "Unknown"
},
"species_markers": [
{
"field": "Clinical Source",
"value": "Genitourinary tract",
"importance": "medium",
"notes": "Isolation from vaginal specimens supports A. vaginalis."
}
],
"common_confusions": [
{
"species": "Anaerococcus prevotii",
"reason": "Very similar biochemical behavior; clinical context often required."
}
],
"when_to_question_identification": [
"Indole consistently negative isolates may fit better with A. prevotii.",
"Aerobic growth argues against Anaerococcus."
],
"recommended_next_tests": [
{
"test": "MALDI-TOF MS",
"reason": "Preferred method for accurate species-level identification."
},
{
"test": "16S rRNA sequencing",
"reason": "Useful in ambiguous anaerobic coccal identifications."
}
]
}