Update data/rag/knowledge_base/Cryptococcus/gattii.json

data/rag/knowledge_base/Cryptococcus/gattii.json

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+{
+  "genus": "Cryptococcus",
+  "species": "gattii",
+  "full_name": "Cryptococcus gattii",
+  "clinical_significance": "A highly virulent Cryptococcus species complex that infects immunocompetent hosts. Often causes large pulmonary or CNS cryptococcomas, and outbreaks have occurred in Canada, the U.S. Pacific Northwest, and Australia.",
+  "phenotype_profile": {
+    "morphology": "Encapsulated yeast; often features a larger and more robust capsule than C. neoformans.",
+    "culture": "Mucoid colonies; blue coloration on CGB (L-canavanine–glycine–bromothymol blue) medium due to ability to utilize glycine.",
+    "biochemical": [
+      "Urease positive",
+      "Phenol oxidase positive",
+      "Grows at 37 °C",
+      "Positive on CGB agar (turns medium blue)",
+      "No pseudohyphae",
+      "Strong melanin production"
+    ]
+  },
+  "differentiation": {
+    "from close species": [
+      "Blue color change on CGB agar is highly specific for C. gattii.",
+      "More frequent disease in immunocompetent hosts vs. C. neoformans.",
+      "Associated with tree habitats (eucalyptus, rainforest species)."
+    ],
+    "from other genera": [
+      "Melanin-producing, encapsulated, but CGB-positive profile is unique."
+    ]
+  },
+  "virulence_factors": [
+    "Large polysaccharide capsule",
+    "Melanin production",
+    "Ability to form large cryptococcomas",
+    "Survival and replication in macrophages",
+    "Enhanced resistance to oxidative stress"
+  ],
+  "confirmation_methods": [
+    "CGB agar",
+    "MALDI-TOF MS",
+    "MLST / molecular typing",
+    "ITS sequencing"
+  ],
+  "treatment_notes": [
+    "Requires longer antifungal therapy than C. neoformans",
+    "Amphotericin B + flucytosine induction",
+    "Prolonged fluconazole consolidation phase",
+    "Surgical resection sometimes required for cryptococcomas"
+  ],
+  "notes": "Major outbreak pathogen with environmental reservoirs in western Canada and the Pacific Northwest.",
+  "references": [
+    "C. gattii outbreak epidemiology",
+    "Treatment outcomes literature"
+  ]
+}