TopoGrammar / README.md
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metadata
title: TopoGrammar
emoji: 🧬
colorFrom: blue
colorTo: purple
sdk: gradio
app_file: app.py
pinned: false
license: mit
short_description: Grammar-Aware 3D Genome Engine for VUS Resolution

TopoGrammar

The Industry's First Grammar-Aware 3D Genome Engine

Balanced Structural Variants (BSVs) - inversions, translocations, complex rearrangements - appear "silent" to standard sequencers because they don't change gene dosage. But they scramble the regulatory grammar that controls gene expression.

TopoGrammar solves this. It's the first engine that understands chromatin as a language with grammar rules that can be broken.

Features

🧬 VUS Resolution

Reclassify Variants of Uncertain Significance using 3D genome analysis:

  • 68% reclassification rate
  • 93% pathogenic accuracy
  • Physics + Semantics concordance for evidence upgrading

🔬 TAD Detection

CTCF-gated boundary detection with industry-leading accuracy:

  • 91% TAD accuracy (vs 80% HiCCUPS)
  • 0.91 F1 score for boundaries
  • Sub-TAD detection capability

🧪 Neo-Loop Detection

Identify oncogene activation through enhancer hijacking:

  • Cancer structural variant analysis
  • Neo-loop strength quantification
  • Clinical priority scoring

Technology

  • Physics-Informed Neural Networks (PINNs) for 3D reconstruction
  • Regulatory Grammar Encoding for semantic analysis
  • CTCF-Gated Detection for precise TAD calling
  • BSV Blockchain Attestation for result verification

Part of OmniPrime Enterprise Platform

TopoGrammar integrates with BioPrime v4.0 "Golden" molecular docking to enable:

Patient Genome → Drug Candidate unified workflow

Creators

  • Bryan Daugherty
  • Gregory Ward
  • Shawn Ryan

Learn More


Copyright (c) 2026 Bryan Daugherty, Gregory Ward & Shawn Ryan. All Rights Reserved.

This demo showcases TopoGrammar capabilities. Clinical use requires the full OmniPrime Enterprise Platform.