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<!DOCTYPE html>
<html lang="en">
<head>
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    <title>Chemistry Batch 12: Spectroscopy & Analysis - Programming Framework</title>
    <style>
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        }
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</head>
<body>
    <div class="container">
        <h1>Chemistry Batch 12: Spectroscopy & Analysis</h1>
        
        <div class="navigation">
            <a href="index.html">← Back to Programming Framework</a>
            <a href="chemistry_index.html">← Back to Chemistry Index</a>
        </div>

        <div class="process-title">Process 1: UV-Vis Spectroscopy</div>
        <div class="figure">
            <div class="mermaid">
graph TD
    A1[Light Source] --> B1[UV-Vis Spectroscopy Method]
    C1[Sample Solution] --> D1[Wavelength Control]
    E1[Detector System] --> F1[Data Collection]
    
    B1 --> G1[Monochromator Setup]
    D1 --> H1[Wavelength Selection]
    F1 --> I1[Signal Processing]
    
    G1 --> J1[Light Dispersion]
    H1 --> K1[Wavelength Range]
    I1 --> L1[Absorbance Measurement]
    
    J1 --> M1[Light Transmission]
    K1 --> L1
    L1 --> N1[Electronic Transitions]
    
    M1 --> O1[Sample Irradiation]
    N1 --> P1[Absorption Process]
    O1 --> Q1[Spectroscopic Analysis]
    
    P1 --> R1[Absorbance Spectrum]
    Q1 --> S1[Peak Analysis]
    R1 --> T1[Concentration Determination]
    
    S1 --> U1[Beer's Law Analysis]
    T1 --> V1[Concentration Calculation]
    U1 --> W1[Spectroscopic Efficiency]
    
    V1 --> X1[Sample Characterization]
    W1 --> Y1[Process Optimization]
    X1 --> Z1[Final Absorption Spectrum]
    
    Y1 --> Z1
    
    style A1 fill:#ff6b6b,color:#fff
    style C1 fill:#ff6b6b,color:#fff
    style E1 fill:#ff6b6b,color:#fff
    
    style B1 fill:#ffd43b,color:#000
    style D1 fill:#ffd43b,color:#000
    style F1 fill:#ffd43b,color:#000
    style G1 fill:#ffd43b,color:#000
    style H1 fill:#ffd43b,color:#000
    style I1 fill:#ffd43b,color:#000
    style J1 fill:#ffd43b,color:#000
    style K1 fill:#ffd43b,color:#000
    style L1 fill:#ffd43b,color:#000
    
    style M1 fill:#51cf66,color:#fff
    style N1 fill:#51cf66,color:#fff
    style O1 fill:#51cf66,color:#fff
    style P1 fill:#51cf66,color:#fff
    style Q1 fill:#51cf66,color:#fff
    style R1 fill:#51cf66,color:#fff
    style S1 fill:#51cf66,color:#fff
    style T1 fill:#51cf66,color:#fff
    style U1 fill:#51cf66,color:#fff
    style V1 fill:#51cf66,color:#fff
    style W1 fill:#51cf66,color:#fff
    style X1 fill:#51cf66,color:#fff
    style Y1 fill:#51cf66,color:#fff
    
    style Z1 fill:#b197fc,color:#fff
            </div>
            <div class="figure-caption">
                <strong>Figure 1.</strong> UV-Vis spectroscopy process showing electronic transitions, absorbance measurement, and concentration determination.
            </div>
        </div>

        <div class="process-title">Process 2: IR Spectroscopy</div>
        <div class="figure">
            <div class="mermaid">
graph TD
    A2[IR Radiation] --> B2[IR Spectroscopy Method]
    C2[Molecular Sample] --> D2[Wavelength Control]
    E2[Detector System] --> F2[Data Collection]
    
    B2 --> G2[IR Source Setup]
    D2 --> H2[Wavelength Selection]
    F2 --> I2[Signal Processing]
    
    G2 --> J2[Radiation Generation]
    H2 --> K2[Frequency Range]
    I2 --> L2[Transmittance Measurement]
    
    J2 --> M2[Radiation Transmission]
    K2 --> L2
    L2 --> N2[Molecular Vibrations]
    
    M2 --> O2[Sample Irradiation]
    N2 --> P2[Vibrational Absorption]
    O2 --> Q2[Spectroscopic Analysis]
    
    P2 --> R2[IR Spectrum]
    Q2 --> S2[Peak Assignment]
    R2 --> T2[Functional Group Analysis]
    
    S2 --> U2[Vibrational Analysis]
    T2 --> V2[Group Identification]
    U2 --> W2[Spectroscopic Efficiency]
    
    V2 --> X2[Molecular Characterization]
    W2 --> Y2[Process Optimization]
    X2 --> Z2[Final IR Spectrum]
    
    Y2 --> Z2
    
    style A2 fill:#ff6b6b,color:#fff
    style C2 fill:#ff6b6b,color:#fff
    style E2 fill:#ff6b6b,color:#fff
    
    style B2 fill:#ffd43b,color:#000
    style D2 fill:#ffd43b,color:#000
    style F2 fill:#ffd43b,color:#000
    style G2 fill:#ffd43b,color:#000
    style H2 fill:#ffd43b,color:#000
    style I2 fill:#ffd43b,color:#000
    style J2 fill:#ffd43b,color:#000
    style K2 fill:#ffd43b,color:#000
    style L2 fill:#ffd43b,color:#000
    
    style M2 fill:#51cf66,color:#fff
    style N2 fill:#51cf66,color:#fff
    style O2 fill:#51cf66,color:#fff
    style P2 fill:#51cf66,color:#fff
    style Q2 fill:#51cf66,color:#fff
    style R2 fill:#51cf66,color:#fff
    style S2 fill:#51cf66,color:#fff
    style T2 fill:#51cf66,color:#fff
    style U2 fill:#51cf66,color:#fff
    style V2 fill:#51cf66,color:#fff
    style W2 fill:#51cf66,color:#fff
    style X2 fill:#51cf66,color:#fff
    style Y2 fill:#51cf66,color:#fff
    
    style Z2 fill:#b197fc,color:#fff
            </div>
            <div class="figure-caption">
                <strong>Figure 2.</strong> IR spectroscopy process illustrating molecular vibrations, functional group analysis, and molecular characterization.
            </div>
        </div>

        <div class="process-title">Process 3: NMR Spectroscopy</div>
        <div class="figure">
            <div class="mermaid">
graph TD
    A3[Magnetic Field] --> B3[NMR Spectroscopy Method]
    C3[Nuclear Sample] --> D3[Radiofrequency Control]
    E3[Detector System] --> F3[Data Collection]
    
    B3 --> G3[Magnet Setup]
    D3 --> H3[RF Pulse Control]
    F3 --> I3[Signal Processing]
    
    G3 --> J3[Field Alignment]
    H3 --> K3[Pulse Sequence]
    I3 --> L3[Free Induction Decay]
    
    J3 --> M3[Nuclear Alignment]
    K3 --> L3
    L3 --> N3[Nuclear Spin Transitions]
    
    M3 --> O3[Sample Irradiation]
    N3 --> P3[Spin Relaxation]
    O3 --> Q3[Spectroscopic Analysis]
    
    P3 --> R3[NMR Spectrum]
    Q3 --> S3[Chemical Shift Analysis]
    R3 --> T3[Molecular Structure]
    
    S3 --> U3[Spin Analysis]
    T3 --> V3[Structure Determination]
    U3 --> W3[Spectroscopic Efficiency]
    
    V3 --> X3[Molecular Characterization]
    W3 --> Y3[Process Optimization]
    X3 --> Z3[Final NMR Spectrum]
    
    Y3 --> Z3
    
    style A3 fill:#ff6b6b,color:#fff
    style C3 fill:#ff6b6b,color:#fff
    style E3 fill:#ff6b6b,color:#fff
    
    style B3 fill:#ffd43b,color:#000
    style D3 fill:#ffd43b,color:#000
    style F3 fill:#ffd43b,color:#000
    style G3 fill:#ffd43b,color:#000
    style H3 fill:#ffd43b,color:#000
    style I3 fill:#ffd43b,color:#000
    style J3 fill:#ffd43b,color:#000
    style K3 fill:#ffd43b,color:#000
    style L3 fill:#ffd43b,color:#000
    
    style M3 fill:#51cf66,color:#fff
    style N3 fill:#51cf66,color:#fff
    style O3 fill:#51cf66,color:#fff
    style P3 fill:#51cf66,color:#fff
    style Q3 fill:#51cf66,color:#fff
    style R3 fill:#51cf66,color:#fff
    style S3 fill:#51cf66,color:#fff
    style T3 fill:#51cf66,color:#fff
    style U3 fill:#51cf66,color:#fff
    style V3 fill:#51cf66,color:#fff
    style W3 fill:#51cf66,color:#fff
    style X3 fill:#51cf66,color:#fff
    style Y3 fill:#51cf66,color:#fff
    
    style Z3 fill:#b197fc,color:#fff
            </div>
            <div class="figure-caption">
                <strong>Figure 3.</strong> NMR spectroscopy process showing nuclear spin transitions, chemical shift analysis, and molecular structure determination.
            </div>
        </div>

        <div class="process-title">Process 4: Mass Spectrometry</div>
        <div class="figure">
            <div class="mermaid">
graph TD
    A4[Sample Molecules] --> B4[Mass Spectrometry Method]
    C4[Ionization Source] --> D4[Mass Analyzer]
    E4[Detector System] --> F4[Data Collection]
    
    B4 --> G4[Sample Introduction]
    D4 --> H4[Mass Separation]
    F4 --> I4[Signal Processing]
    
    G4 --> J4[Sample Vaporization]
    H4 --> K4[Mass-to-Charge Ratio]
    I4 --> L4[Ion Detection]
    
    J4 --> M4[Molecular Ionization]
    K4 --> L4
    L4 --> N4[Ion Separation]
    
    M4 --> O4[Ion Formation]
    N4 --> P4[Mass Analysis]
    O4 --> Q4[Spectroscopic Analysis]
    
    P4 --> R4[Mass Spectrum]
    Q4 --> S4[Fragmentation Analysis]
    R4 --> T4[Molecular Weight]
    
    S4 --> U4[Fragmentation Pattern]
    T4 --> V4[Molecular Identification]
    U4 --> W4[Spectroscopic Efficiency]
    
    V4 --> X4[Molecular Characterization]
    W4 --> Y4[Process Optimization]
    X4 --> Z4[Final Mass Spectrum]
    
    Y4 --> Z4
    
    style A4 fill:#ff6b6b,color:#fff
    style C4 fill:#ff6b6b,color:#fff
    style E4 fill:#ff6b6b,color:#fff
    
    style B4 fill:#ffd43b,color:#000
    style D4 fill:#ffd43b,color:#000
    style F4 fill:#ffd43b,color:#000
    style G4 fill:#ffd43b,color:#000
    style H4 fill:#ffd43b,color:#000
    style I4 fill:#ffd43b,color:#000
    style J4 fill:#ffd43b,color:#000
    style K4 fill:#ffd43b,color:#000
    style L4 fill:#ffd43b,color:#000
    
    style M4 fill:#51cf66,color:#fff
    style N4 fill:#51cf66,color:#fff
    style O4 fill:#51cf66,color:#fff
    style P4 fill:#51cf66,color:#fff
    style Q4 fill:#51cf66,color:#fff
    style R4 fill:#51cf66,color:#fff
    style S4 fill:#51cf66,color:#fff
    style T4 fill:#51cf66,color:#fff
    style U4 fill:#51cf66,color:#fff
    style V4 fill:#51cf66,color:#fff
    style W4 fill:#51cf66,color:#fff
    style X4 fill:#51cf66,color:#fff
    style Y4 fill:#51cf66,color:#fff
    
    style Z4 fill:#b197fc,color:#fff
            </div>
            <div class="figure-caption">
                <strong>Figure 4.</strong> Mass spectrometry process showing ionization, fragmentation, and molecular weight determination.
            </div>
        </div>

        <div class="process-title">Process 5: Chromatography</div>
        <div class="figure">
            <div class="mermaid">
graph TD
    A5[Sample Mixture] --> B5[Chromatography Method]
    C5[Stationary Phase] --> D5[Mobile Phase]
    E5[Detector System] --> F5[Data Collection]
    
    B5 --> G5[Column Preparation]
    D5 --> H5[Flow Control]
    F5 --> I5[Signal Processing]
    
    G5 --> J5[Column Packing]
    H5 --> K5[Flow Rate]
    I5 --> L5[Retention Time]
    
    J5 --> M5[Sample Injection]
    K5 --> L5
    L5 --> N5[Separation Process]
    
    M5 --> O5[Sample Loading]
    N5 --> P5[Chromatographic Separation]
    O5 --> Q5[Analytical Process]
    
    P5 --> R5[Chromatogram]
    Q5 --> S5[Peak Analysis]
    R5 --> T5[Component Separation]
    
    S5 --> U5[Retention Analysis]
    T5 --> V5[Component Identification]
    U5 --> W5[Chromatographic Efficiency]
    
    V5 --> X5[Sample Characterization]
    W5 --> Y5[Process Optimization]
    X5 --> Z5[Final Chromatogram]
    
    Y5 --> Z5
    
    style A5 fill:#ff6b6b,color:#fff
    style C5 fill:#ff6b6b,color:#fff
    style E5 fill:#ff6b6b,color:#fff
    
    style B5 fill:#ffd43b,color:#000
    style D5 fill:#ffd43b,color:#000
    style F5 fill:#ffd43b,color:#000
    style G5 fill:#ffd43b,color:#000
    style H5 fill:#ffd43b,color:#000
    style I5 fill:#ffd43b,color:#000
    style J5 fill:#ffd43b,color:#000
    style K5 fill:#ffd43b,color:#000
    style L5 fill:#ffd43b,color:#000
    
    style M5 fill:#51cf66,color:#fff
    style N5 fill:#51cf66,color:#fff
    style O5 fill:#51cf66,color:#fff
    style P5 fill:#51cf66,color:#fff
    style Q5 fill:#51cf66,color:#fff
    style R5 fill:#51cf66,color:#fff
    style S5 fill:#51cf66,color:#fff
    style T5 fill:#51cf66,color:#fff
    style U5 fill:#51cf66,color:#fff
    style V5 fill:#51cf66,color:#fff
    style W5 fill:#51cf66,color:#fff
    style X5 fill:#51cf66,color:#fff
    style Y5 fill:#51cf66,color:#fff
    
    style Z5 fill:#b197fc,color:#fff
            </div>
            <div class="figure-caption">
                <strong>Figure 5.</strong> Chromatography process showing separation, retention analysis, and component identification.
            </div>
        </div>

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        <p style="margin-bottom: 0.5rem;"><strong>Gary Welz</strong></p>
        <p style="margin-bottom: 0.5rem;">Retired Faculty Member</p>
        <p style="margin-bottom: 0.5rem;">John Jay College, CUNY (Department of Mathematics and Computer Science)</p>
        <p style="margin-bottom: 0.5rem;">Borough of Manhattan Community College, CUNY</p>
        <p style="margin-bottom: 0.5rem;">CUNY Graduate Center (New Media Lab)</p>
        <p style="margin-bottom: 0.5rem;">Email: gwelz@jjay.cuny.edu</p>
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